FastQCFastQC Report
Fri 27 May 2016
SRR939169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3131627
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69530.222025164555038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53330.17029486589558718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43960.1403743166092258No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36870.1177343278749353No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG112900.026.4885981
TACATGG111200.026.0278472
ACATGGG115000.023.8908713
AGTACAT84850.019.4838772
CATGGGG66350.019.0621624
GTATCAA160950.019.0525841
GAGTACA98700.018.5163751
GAGTACT62950.018.46706812-13
GTGTAGC17750.017.917971
TACCGTA1851.5587154E-417.9177047
TACCTGG27350.017.8394622
GTATAGC11650.017.5208381
AGAGTAC127950.017.41195910-11
AGTACTT67200.017.36959812-13
CATGGGA56250.017.263674
ACCGTAT2202.99801E-517.2196128
GTACTTT66700.017.21679714-15
CAACGCA177350.017.0141435
ATCAACG178950.016.9414313
TCAACGC178650.016.916854
Produced by FastQC (version 0.11.5)