##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939169_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3131627 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12207647973402 33.0 31.0 34.0 30.0 34.0 2 32.21355384916531 34.0 31.0 34.0 30.0 34.0 3 32.28742727023365 34.0 31.0 34.0 30.0 34.0 4 35.712477890885474 37.0 35.0 37.0 35.0 37.0 5 35.75705855135366 37.0 35.0 37.0 35.0 37.0 6 35.83754291299698 37.0 37.0 37.0 35.0 37.0 7 35.76176217665769 37.0 36.0 37.0 35.0 37.0 8 35.751517980908964 37.0 36.0 37.0 35.0 37.0 9 37.44036087311803 39.0 38.0 39.0 35.0 39.0 10-11 37.353439282519915 39.0 37.5 39.0 34.5 39.0 12-13 37.26613881538255 39.0 37.0 39.0 34.0 39.0 14-15 38.60653775178206 40.0 38.0 41.0 34.0 41.0 16-17 38.54257036358417 40.0 38.0 41.0 34.0 41.0 18-19 38.45262255051448 40.0 38.0 41.0 34.0 41.0 20-21 38.343859437921566 40.0 38.0 41.0 34.0 41.0 22-23 38.266461970087754 40.0 38.0 41.0 34.0 41.0 24-25 38.316314650499564 40.0 38.0 41.0 34.0 41.0 26-27 38.24114429975218 40.0 38.0 41.0 34.0 41.0 28-29 38.13025864830007 40.0 38.0 41.0 33.5 41.0 30-31 37.99612421913593 40.0 38.0 41.0 33.0 41.0 32-33 37.83027081449994 40.0 38.0 41.0 33.0 41.0 34-35 37.538528375186445 40.0 37.5 41.0 32.5 41.0 36-37 37.305052613226295 40.0 37.0 41.0 31.0 41.0 38-39 37.08276001580009 40.0 37.0 41.0 31.0 41.0 40-41 36.95742596420327 40.0 37.0 41.0 31.0 41.0 42-43 36.786039333547706 40.0 36.5 41.0 30.0 41.0 44-45 36.56230659015266 40.0 36.0 41.0 30.0 41.0 46-47 36.263412756372325 39.0 36.0 41.0 29.5 41.0 48-49 36.31025454180846 40.0 36.0 41.0 30.0 41.0 50-51 35.32886898727084 38.5 34.5 40.0 27.5 40.5 52-53 35.491520541878074 38.5 35.0 40.0 28.0 40.5 54-55 36.01125804573789 39.0 35.0 41.0 29.0 41.0 56-57 35.892288577151746 39.0 35.0 41.0 28.0 41.0 58-59 35.70519365812085 39.0 35.0 41.0 28.0 41.0 60-61 35.4077873897498 39.0 35.0 41.0 28.0 41.0 62-63 35.04868475715658 38.0 35.0 40.0 27.5 41.0 64-65 34.670496039279264 37.5 34.0 40.0 26.0 41.0 66-67 34.27704129514785 37.0 34.0 40.0 26.0 41.0 68-69 33.870698521886546 36.0 34.0 39.0 26.0 41.0 70-71 33.4673030983575 36.0 34.0 39.0 25.0 41.0 72-73 33.01912105113412 35.0 33.0 38.5 24.5 40.0 74-75 32.57668873080989 35.0 33.0 37.0 23.5 39.5 76-77 32.145787317582844 35.0 32.5 37.0 22.0 39.0 78-79 31.73562719953558 35.0 32.0 36.0 20.5 39.0 80-81 31.327609578024457 35.0 32.0 36.0 20.0 37.5 82-83 30.924236187770767 35.0 31.0 35.5 18.0 37.0 84-85 30.56182297572476 34.5 31.0 35.0 16.0 37.0 86-87 30.199349092340817 34.0 31.0 35.0 9.0 36.0 88-89 29.83181521937319 34.0 30.5 35.0 4.5 36.0 90-91 29.478985683799507 34.0 30.0 35.0 2.0 35.5 92-93 29.09369043631314 34.0 30.0 35.0 2.0 35.0 94-95 28.669764311011498 34.0 29.0 35.0 2.0 35.0 96-97 28.139105966323577 34.0 29.0 35.0 2.0 35.0 98-99 27.468926056647234 34.0 28.0 35.0 2.0 35.0 100-101 24.937205644222637 31.5 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10961697006721494 1101 2 0.20605421097811671 1101 3 0.20067866521070954 1101 4 0.12499437668986246 1101 5 0.1206509136315077 1101 6 0.1718600642865269 1101 7 0.05852568555750537 1101 8 0.23071476877959185 1101 9 0.3368383856866686 1101 10-11 0.2939228254610313 1101 12-13 0.21770187699809895 1101 14-15 0.2261594487183345 1101 16-17 0.3571305594617016 1101 18-19 0.3556500306646342 1101 20-21 0.4595104541201991 1101 22-23 0.5304396445673589 1101 24-25 0.41745794758840304 1101 26-27 0.435198657882772 1101 28-29 0.38890455515871736 1101 30-31 0.4698885961844539 1101 32-33 0.34356491091636343 1101 34-35 0.1844490518501587 1101 36-37 0.06367694489826903 1101 38-39 0.1402315274712862 1101 40-41 0.06178390511295362 1101 42-43 -0.025823839372854707 1101 44-45 -0.042486723842927177 1101 46-47 -0.12163413734977269 1101 48-49 0.039836312505840965 1101 50-51 0.021418990029275164 1101 52-53 0.08420352669247677 1101 54-55 0.06265577009689594 1101 56-57 0.09510700367684422 1101 58-59 0.1250446053914871 1101 60-61 -0.04889321603162955 1101 62-63 -0.003499076844434512 1101 64-65 -0.0726121901219301 1101 66-67 -0.166624787723606 1101 68-69 -0.15647932364797157 1101 70-71 -0.1435758655465662 1101 72-73 -0.24472018402825313 1101 74-75 -0.38165872647866195 1101 76-77 -0.3495461817072112 1101 78-79 -0.2630721694325793 1101 80-81 -0.43135006562132006 1101 82-83 -0.3892131589511827 1101 84-85 -0.38072289427684325 1101 86-87 -0.3579104879372288 1101 88-89 -0.42710002596196617 1101 90-91 -0.6474800564638734 1101 92-93 -0.8377669156443268 1101 94-95 -1.0606560629953528 1101 96-97 -0.7847787939861028 1101 98-99 -1.0428605877442116 1101 100-101 -1.1805505188684968 1102 1 0.1916453467821313 1102 2 0.18327053267780968 1102 3 0.243740752803852 1102 4 0.2425838421211921 1102 5 0.22129137939346322 1102 6 0.2968196784347157 1102 7 0.29930249933995867 1102 8 0.35204907963003507 1102 9 0.43473274814484597 1102 10-11 0.24027742347102787 1102 12-13 0.39637696258918 1102 14-15 0.39337020255526056 1102 16-17 0.4474434878882789 1102 18-19 0.49982583889322996 1102 20-21 0.48603379498863575 1102 22-23 0.4847411685678651 1102 24-25 0.41073356864203703 1102 26-27 0.49879775883986355 1102 28-29 0.4606662425549928 1102 30-31 0.5325833232999457 1102 32-33 0.5033728708062171 1102 34-35 0.4279308426772914 1102 36-37 0.48009903035903534 1102 38-39 0.4432935558318505 1102 40-41 0.28822825125524787 1102 42-43 0.43920578230325447 1102 44-45 0.40850497767223715 1102 46-47 0.26369414039287875 1102 48-49 0.375468318778033 1102 50-51 0.4428351899252405 1102 52-53 0.40417968827034656 1102 54-55 0.5395378988728439 1102 56-57 0.5721399062995545 1102 58-59 0.5121923330680715 1102 60-61 0.4168586392944036 1102 62-63 0.38126029886568347 1102 64-65 0.3960032913366547 1102 66-67 0.3699666786920517 1102 68-69 0.25879587093106693 1102 70-71 0.09370339884937096 1102 72-73 0.13175342839464577 1102 74-75 0.07781090766859222 1102 76-77 0.20266491675565135 1102 78-79 0.10185627222278626 1102 80-81 0.17803588904098433 1102 82-83 -0.06231075647763262 1102 84-85 -0.14738387551303944 1102 86-87 -0.15199137595211099 1102 88-89 -0.004289571841912476 1102 90-91 -0.06271634600566145 1102 92-93 -0.2515415065894935 1102 94-95 -0.3799629636989259 1102 96-97 -0.45845592848606387 1102 98-99 -0.6422568327215608 1102 100-101 -0.741142018131324 1103 1 0.186821828248938 1103 2 0.21455860961104634 1103 3 0.2618401425161494 1103 4 0.24468959890436537 1103 5 0.25351245677583023 1103 6 0.20532103903284593 1103 7 0.22671564562407553 1103 8 0.17305711112193478 1103 9 0.30584038070409036 1103 10-11 0.3356221303479927 1103 12-13 0.3590447917086337 1103 14-15 0.4098602556582094 1103 16-17 0.38844088557922873 1103 18-19 0.4022953245078753 1103 20-21 0.4272218085153554 1103 22-23 0.41554933744054523 1103 24-25 0.34106922688091856 1103 26-27 0.31685012205351626 1103 28-29 0.3761352526207773 1103 30-31 0.34315873742374237 1103 32-33 0.3529860250161434 1103 34-35 0.2751411541617941 1103 36-37 0.1896928984206525 1103 38-39 0.23015348027242055 1103 40-41 0.24081015133714345 1103 42-43 0.09217558495047484 1103 44-45 0.162909947157857 1103 46-47 0.1347354603051727 1103 48-49 0.19255082650569477 1103 50-51 0.29564720281789647 1103 52-53 0.3678819682629495 1103 54-55 0.3219776626835724 1103 56-57 0.3123667827361132 1103 58-59 0.377349330241195 1103 60-61 0.35342715795969326 1103 62-63 0.23977486915445212 1103 64-65 0.12129179097999554 1103 66-67 0.18362523460866953 1103 68-69 0.023432604438767157 1103 70-71 0.15148628437418665 1103 72-73 0.03531878994387938 1103 74-75 0.061910601203770455 1103 76-77 0.10259490539531413 1103 78-79 0.016660372510759913 1103 80-81 0.038275446780541955 1103 82-83 0.10855745043896192 1103 84-85 0.1409376351471856 1103 86-87 0.06489034447799824 1103 88-89 0.10206752572486621 1103 90-91 0.038519288455777456 1103 92-93 -0.029399951544714753 1103 94-95 -0.19612270848770663 1103 96-97 -0.2914918456348836 1103 98-99 -0.6392176131500804 1103 100-101 -0.5962094536019826 1104 1 0.16987446451258137 1104 2 0.07062160273826379 1104 3 0.1519521034537945 1104 4 0.06832753465239705 1104 5 0.14787308416398304 1104 6 0.1762899048640989 1104 7 0.1254378540710519 1104 8 0.17284603201176907 1104 9 0.14574425069969976 1104 10-11 0.19079954488319828 1104 12-13 0.20547501162509008 1104 14-15 0.2510185840642478 1104 16-17 0.2538473718114034 1104 18-19 0.279968177318338 1104 20-21 0.23085694682939817 1104 22-23 0.16912248716048595 1104 24-25 0.2769007323454531 1104 26-27 0.35713348940110023 1104 28-29 0.31191182665423156 1104 30-31 0.19066946621887837 1104 32-33 0.1928329926612804 1104 34-35 0.20104990695128322 1104 36-37 0.1550981766699593 1104 38-39 0.2943674966882668 1104 40-41 0.11214802414291114 1104 42-43 0.05989879157275624 1104 44-45 0.037851677879402246 1104 46-47 -0.0019457021558082488 1104 48-49 0.10684806658275647 1104 50-51 0.11522256505380568 1104 52-53 0.16662000764318918 1104 54-55 0.10762568190957467 1104 56-57 0.16905754548191254 1104 58-59 0.11575943406415234 1104 60-61 0.14083069417056748 1104 62-63 0.14492837834465178 1104 64-65 0.04746661788876594 1104 66-67 0.06059389699018425 1104 68-69 0.01516154580073703 1104 70-71 -0.07116517442405268 1104 72-73 -0.018134324426668513 1104 74-75 -0.05550622281855766 1104 76-77 0.06636336687019906 1104 78-79 0.055752923071199945 1104 80-81 0.015540700743041924 1104 82-83 -0.012596173191724347 1104 84-85 0.0010288060640384344 1104 86-87 -0.1523385428426316 1104 88-89 -0.16264074641459914 1104 90-91 -0.08437987040668204 1104 92-93 -0.27437639587895646 1104 94-95 -0.2942964291384911 1104 96-97 -0.21500643997516988 1104 98-99 -0.2051032235311041 1104 100-101 -0.5636550149007569 1105 1 0.1703308341705494 1105 2 0.1327159307430108 1105 3 0.14497668550190212 1105 4 0.24925017044232334 1105 5 0.16326243979517585 1105 6 0.1607919588903357 1105 7 0.14890497683328618 1105 8 0.12824732532516947 1105 9 0.22140898563527145 1105 10-11 0.2046693612663688 1105 12-13 0.2307586645945392 1105 14-15 0.27564077915635465 1105 16-17 0.16712818837207521 1105 18-19 0.19309094535147153 1105 20-21 0.28331829568106315 1105 22-23 0.21911261970841878 1105 24-25 0.13527147573704212 1105 26-27 0.255772869338152 1105 28-29 0.21733822392656066 1105 30-31 0.20179997633228908 1105 32-33 0.2888316924793628 1105 34-35 0.21718198182976067 1105 36-37 0.265691515269161 1105 38-39 0.2920051125764189 1105 40-41 0.274726631216609 1105 42-43 0.18319998151975625 1105 44-45 0.20731077704874679 1105 46-47 0.24465994270395441 1105 48-49 0.3592538626467032 1105 50-51 0.2382840823293506 1105 52-53 0.22484909989081103 1105 54-55 0.21942185807841952 1105 56-57 0.18814489806871393 1105 58-59 0.15174818420139502 1105 60-61 0.2981840258625539 1105 62-63 0.18321082738820849 1105 64-65 0.21266481582114238 1105 66-67 0.16786434692181018 1105 68-69 0.16187520056470817 1105 70-71 -0.05666171283480992 1105 72-73 -0.14661169434438648 1105 74-75 -0.08577342277908429 1105 76-77 0.051359639972254456 1105 78-79 0.025298894514598658 1105 80-81 -0.04074331838626577 1105 82-83 0.060684169344295213 1105 84-85 0.16722257670285856 1105 86-87 0.07171465336536542 1105 88-89 0.09899655893902448 1105 90-91 0.1666668534299376 1105 92-93 0.25534265260844435 1105 94-95 0.10000199743823757 1105 96-97 0.10886147547237357 1105 98-99 -0.010120798720844704 1105 100-101 0.07375010156662043 1106 1 0.22112898532169112 1106 2 0.2572354854751069 1106 3 0.25319514000942434 1106 4 0.22042391669602068 1106 5 0.27258481750080676 1106 6 0.2557277357801837 1106 7 0.21387636452172387 1106 8 0.22396940621635508 1106 9 0.3324882089708012 1106 10-11 0.2620909938151712 1106 12-13 0.3095739380556495 1106 14-15 0.3435305532745474 1106 16-17 0.3897057929824115 1106 18-19 0.3640080996776476 1106 20-21 0.3174917749404642 1106 22-23 0.36649110551554287 1106 24-25 0.42300441670818145 1106 26-27 0.39591367126883625 1106 28-29 0.3557405577572794 1106 30-31 0.3021013562651902 1106 32-33 0.31910485885841666 1106 34-35 0.3132743294956484 1106 36-37 0.3495701449758073 1106 38-39 0.45141382999820223 1106 40-41 0.43511426995829083 1106 42-43 0.3705603440772265 1106 44-45 0.40273402080993037 1106 46-47 0.3794101180995568 1106 48-49 0.3834245332647299 1106 50-51 0.35368618615198955 1106 52-53 0.32137603568619255 1106 54-55 0.31716748994760025 1106 56-57 0.35903101864149534 1106 58-59 0.3391854665461338 1106 60-61 0.4042735186542856 1106 62-63 0.5026665635469882 1106 64-65 0.3378899405629596 1106 66-67 0.2718782768583452 1106 68-69 0.3492014086561426 1106 70-71 0.360004279603821 1106 72-73 0.40599438965826096 1106 74-75 0.2666937317204585 1106 76-77 0.30714954435130437 1106 78-79 0.28910313567864776 1106 80-81 0.37610234619260297 1106 82-83 0.4611490977355004 1106 84-85 0.5000406842333689 1106 86-87 0.45142210389339965 1106 88-89 0.5592955463255684 1106 90-91 0.6528887337864369 1106 92-93 0.5371703962060721 1106 94-95 0.4256675471979996 1106 96-97 0.41869669028720935 1106 98-99 0.3666830598669506 1106 100-101 0.41924350357104245 1107 1 0.018119903733058607 1107 2 0.10983082534838218 1107 3 0.13189850652799606 1107 4 0.09841801170254882 1107 5 0.15766378418297222 1107 6 0.15713451363701836 1107 7 0.13632206267235603 1107 8 0.1217446691118198 1107 9 0.1519892538297043 1107 10-11 0.2266526292854678 1107 12-13 0.2903701037316111 1107 14-15 0.30834009762643433 1107 16-17 0.3667480299328787 1107 18-19 0.29405073440281626 1107 20-21 0.21237280668960778 1107 22-23 0.18140429538852487 1107 24-25 0.22621154527904963 1107 26-27 0.23486814007153356 1107 28-29 0.18576890620791886 1107 30-31 0.21533436820403296 1107 32-33 0.12880776037276576 1107 34-35 0.14356263211815445 1107 36-37 0.15326776897226324 1107 38-39 0.27160509800310706 1107 40-41 0.1777593596817013 1107 42-43 0.17423807933855073 1107 44-45 0.25795439359765737 1107 46-47 0.36829147479141255 1107 48-49 0.28291807082643317 1107 50-51 0.28247639080987597 1107 52-53 0.39093814736564525 1107 54-55 0.3282827717793779 1107 56-57 0.22007262811637673 1107 58-59 0.22709731743105266 1107 60-61 0.12229579403763324 1107 62-63 0.20327611126034384 1107 64-65 0.17857921129066767 1107 66-67 0.1257523783637282 1107 68-69 0.18361368252682553 1107 70-71 0.2633329815697252 1107 72-73 0.2745651459344174 1107 74-75 0.3793922689179965 1107 76-77 0.320550069862108 1107 78-79 0.3860716442476111 1107 80-81 0.39530786125481754 1107 82-83 0.4006313183267842 1107 84-85 0.3803746469097753 1107 86-87 0.2790985393838703 1107 88-89 0.2644299213430763 1107 90-91 0.17451731305845541 1107 92-93 0.2976800520469176 1107 94-95 0.2630404413731178 1107 96-97 0.247342071338565 1107 98-99 0.37987041175189873 1107 100-101 0.3710264866572146 1108 1 -0.1804816221086405 1108 2 -0.01724644286410637 1108 3 0.006564996235844944 1108 4 -0.03101544892696495 1108 5 0.04806583747611626 1108 6 0.06896214147549529 1108 7 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-0.8726942716267381 2316 100-101 -0.7003836277761586 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10390.0 3 2643.0 4 2536.0 5 2883.0 6 3304.0 7 3863.0 8 4663.0 9 5610.0 10 6717.0 11 8262.0 12 10506.0 13 15930.0 14 13532.0 15 12849.0 16 15086.0 17 19180.0 18 18469.0 19 13749.0 20 14402.0 21 15371.0 22 16654.0 23 18784.0 24 21712.0 25 24766.0 26 28722.0 27 33299.0 28 39303.0 29 47745.0 30 57721.0 31 71738.0 32 90823.0 33 118677.0 34 166528.0 35 251185.0 36 418606.0 37 701360.0 38 697668.0 39 126347.0 40 44.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.45260427981229 18.201121571507393 13.315121093452117 24.031153055228202 2 15.903713297272551 19.767720302685504 39.465009221939425 24.86355717810252 3 20.25034726051812 23.17846073678372 28.58958681992484 27.981605182773322 4 13.190100590730358 15.441922384994292 36.074579667711376 35.29339735656398 5 14.422412800736733 36.8222951280969 33.72776051059693 15.027531560569432 6 33.73750203352138 34.442626571324745 17.08621395553388 14.733657439619996 7 30.155490662121853 31.384047807481295 20.070173128237126 18.390288402159722 8 26.850125416790664 34.545349349773 19.458286410586474 19.146238822849863 9 26.501705797409947 16.83318726823752 20.01152539902619 36.65358153532634 10-11 25.77887561673538 25.89382483165126 26.749607593775583 21.577691957837786 12-13 26.405538732581192 23.0022744576109 27.43422259639756 23.157964213410352 14-15 23.104968289350964 24.69527923334448 25.462235209208455 26.7375172680961 16-17 23.046833052940542 27.89998798316621 26.113602544500637 22.939576419392612 18-19 22.97631886646623 26.925262795183098 27.69501310859388 22.403405229756796 20-21 23.65053311512272 26.0348500825003 27.58527708128441 22.72933972109257 22-23 23.260203160859046 25.920441265117294 27.54775504645186 23.2716005275718 24-25 23.281706909399375 25.99521566823091 27.728500762829615 22.994576659540105 26-27 23.04482978800278 26.184021248642303 27.82257690626598 22.94857205708894 28-29 22.798827499285412 26.674327311606007 27.714109590705426 22.812735598403158 30-31 22.955853046708995 26.24318908072591 27.566394024757564 23.234563847807536 32-33 22.916000345887415 26.25438932569696 27.682453614083947 23.14715671433168 34-35 23.4596612033203 26.24416724017065 27.398640379038074 22.89753117747097 36-37 23.35963059005242 26.03320909167199 27.55273451195234 23.05442580632325 38-39 23.061930540429852 26.34031085981728 27.665763375597603 22.931995224155262 40-41 23.205339372176308 26.280353926664624 27.525319998267893 22.98898670289117 42-43 22.98453184179149 26.29529140125958 27.292356789266236 23.42781996768269 44-45 23.09375853860343 26.29016242178579 27.33123227199412 23.284846767616656 46-47 23.308877636258785 26.580455574271905 27.00345077566236 23.10721601380695 48-49 23.28911065575941 26.421676467034228 27.09800693854148 23.191205938664886 50-51 22.99467754722712 26.662582552091312 27.283768035342458 23.058971865339103 52-53 23.327814527030064 26.6963670229768 26.828089878986344 23.147728571006787 54-55 22.950578226233812 26.427909393013664 27.088721038752013 23.532791342000507 56-57 23.193493141071716 26.646881445709152 26.92712217267006 23.232503240549075 58-59 23.471790063921116 26.33029470942016 26.917208537313297 23.280706689345422 60-61 23.15725773808999 26.39332108073968 27.409297810964333 23.040123370206 62-63 22.978152994648898 26.468970360793943 27.40856147479117 23.144315169765992 64-65 22.849691943150777 26.55718990275757 27.18169625276933 23.411421901322317 66-67 22.93757370221513 26.567975508878288 26.909822190296634 23.584628598609953 68-69 22.795889905924437 26.47426327964886 27.22325006496355 23.506596749463156 70-71 22.998030449709702 26.366182924230092 27.112836999812544 23.522949626247662 72-73 23.008797721726758 26.221357495748137 27.222996617445943 23.546848165079165 74-75 22.77574491168267 26.773536577145663 26.923867024879073 23.5268514862926 76-77 22.96409385850625 26.39260248124306 26.968146897804363 23.675156762446328 78-79 22.98129282665766 26.33268658045256 27.106592307464023 23.579428285425763 80-81 22.979071142586445 26.504661676714907 27.06485215431098 23.451415026387668 82-83 23.04965022612042 26.362141471946295 27.115421609850305 23.472786692082988 84-85 23.006130982874545 26.36884184220529 26.980929342961367 23.644097831958796 86-87 22.925380308970755 26.324286441342597 27.168385274503397 23.581947975183247 88-89 22.85278684910123 26.309885110001353 27.03622572465972 23.801102316237703 90-91 22.923508274125442 26.231954767151294 27.149531285358226 23.695005673365035 92-93 22.97903480504808 26.164592186517055 27.192679513679373 23.663693494755492 94-95 22.922167378268572 26.041452014536322 27.247805011449184 23.78857559574592 96-97 22.854753897620704 25.875868333481783 27.422671478058657 23.846706290838856 98-99 22.792466370936967 26.022416272772293 27.06238086480557 24.12273649148517 100-101 23.024272068988978 25.42150552679446 27.53614142329559 24.018080980920974 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2741.0 1 1522.5 2 316.0 3 332.0 4 305.5 5 293.5 6 465.0 7 855.0 8 1305.5 9 1616.5 10 1674.5 11 1771.5 12 1977.0 13 2186.0 14 2453.5 15 2724.5 16 3008.0 17 3192.5 18 3295.5 19 3447.0 20 3720.5 21 4027.5 22 4527.0 23 5521.0 24 6874.0 25 8727.0 26 11468.5 27 15088.5 28 18396.5 29 22197.0 30 27170.0 31 33095.5 32 39468.5 33 44505.5 34 49505.5 35 55089.5 36 62276.5 37 69973.5 38 75967.0 39 80663.5 40 85116.5 41 90466.5 42 95533.5 43 101198.0 44 107347.0 45 112935.0 46 116554.5 47 123496.0 48 138918.5 49 139557.5 50 127634.5 51 122756.0 52 120191.0 53 119063.5 54 117657.0 55 111018.0 56 100370.5 57 90488.5 58 82448.0 59 72990.5 60 61586.5 61 50821.0 62 41037.5 63 32555.0 64 24844.5 65 18223.5 66 13341.5 67 10557.0 68 8055.5 69 5731.5 70 4308.0 71 3169.5 72 2467.0 73 1986.0 74 1518.0 75 1078.0 76 737.0 77 469.5 78 296.5 79 193.0 80 123.5 81 83.5 82 65.5 83 56.5 84 39.5 85 29.5 86 23.0 87 15.0 88 17.0 89 13.0 90 8.5 91 12.0 92 13.0 93 12.5 94 8.0 95 2.5 96 3.5 97 3.5 98 3.5 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3400468829780814 2 0.12006538454292288 3 0.11406211531577676 4 0.12147040500034008 5 0.13753234341126833 6 0.08998517384094594 7 0.09831949973607967 8 0.10403537841511777 9 0.09212463681019482 10-11 0.09113473603337818 12-13 0.09480694859253673 14-15 0.09659515644743132 16-17 0.08567431561932504 18-19 0.10042703042220545 20-21 0.11037393661505665 22-23 0.11851666881145169 24-25 0.13176856630754558 26-27 0.1304753088410593 28-29 0.12622831518568461 30-31 0.11666459639031086 32-33 0.10989495236820988 34-35 0.33410747831718146 36-37 0.49566886477859595 38-39 0.469133137503285 40-41 0.44807379678358883 42-43 0.47997414762358354 44-45 0.4874622680159546 46-47 0.4954613049382956 48-49 0.5058871953779936 50-51 0.4945831671524099 52-53 0.49862260096748434 54-55 0.48398164915553477 56-57 0.4625550871799227 58-59 0.4431562251826287 60-61 0.40105350988479793 62-63 0.47235829809871993 64-65 0.5047216670439998 66-67 0.5124812118429175 68-69 0.5242801904569094 70-71 0.5171433251788926 72-73 0.5088089992837589 74-75 0.5159298984202141 76-77 0.49325797740280053 78-79 0.4717835170025038 80-81 0.4735557587158368 82-83 0.4871748774678466 84-85 0.48672782550412297 86-87 0.49000088452424256 88-89 0.5024065765175737 90-91 0.5170475283295233 92-93 0.5105014102892842 94-95 0.514780336227782 96-97 0.5142534535562505 98-99 0.50912832211499 100-101 0.49606801831763486 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3131627.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.93438987730892 #Duplication Level Percentage of deduplicated Percentage of total 1 75.07262443445325 40.48996195360589 2 13.611842148174965 14.682928027361095 3 4.725918373480025 7.646685722508277 4 2.153103682167597 4.645053337611864 5 1.1833182800447488 3.191077473244005 6 0.7216948461423647 2.335450272257206 7 0.4886548117960841 1.844870939438311 8 0.3419377089827493 1.4753761368023517 9 0.24723024775905209 1.2000791314890356 >10 1.3145763261008125 12.90016044015792 >50 0.09364309709238777 3.437603569261105 >100 0.042433938434501425 4.005573610366751 >500 0.0018962229786324688 0.7129291366045639 >1k 0.0010073684566799899 1.0395911123522033 >5k 1.185139360799988E-4 0.39265913693922677 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6953 0.222025164555038 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5333 0.17029486589558718 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4396 0.1403743166092258 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3687 0.1177343278749353 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.193228312311779E-5 0.0 10-11 0.0 0.0 0.0 4.789842468467669E-5 0.0 12-13 0.0 0.0 0.0 7.983070780779447E-5 0.0 14-15 0.0 0.0 0.0 1.4369527405403007E-4 0.0 16-17 0.0 0.0 0.0 1.9159369873870675E-4 0.0 18-19 0.0 0.0 0.0 2.3949212342338344E-4 0.0 20-21 0.0 0.0 0.0 2.8739054810806013E-4 0.0 22-23 0.0 0.0 0.0 5.747810962161203E-4 0.0 24-25 0.0 0.0 0.0 7.344425118317093E-4 0.0 26-27 0.0 0.0 0.0 9.739346352550925E-4 0.0 28-29 1.5966141561558895E-5 0.0 0.0 0.0023789550926722755 0.0 30-31 3.193228312311779E-5 0.0 0.0 0.007232662127386179 0.0 32-33 3.193228312311779E-5 0.0 0.0 0.01662075336558281 0.0 34-35 4.789842468467669E-5 0.0 0.0 0.03435913664047474 0.0 36-37 6.386456624623558E-5 0.0 0.0 0.0663073859051541 0.0 38-39 6.386456624623558E-5 0.0 0.0 0.11249743344274397 0.0 40-41 6.386456624623558E-5 0.0 0.0 0.16235969353949242 0.0 42-43 7.983070780779447E-5 0.0 0.0 0.2153832496654295 0.0 44-45 9.579684936935338E-5 0.0 0.0 0.28360657255797067 0.0 46-47 9.579684936935338E-5 0.0 0.0 0.36779603701207075 0.0 48-49 9.579684936935338E-5 0.0 0.0 0.46319373284238513 0.0 50-51 9.579684936935338E-5 0.0 0.0 0.5620880136746809 0.0 52-53 9.579684936935338E-5 0.0 0.0 0.6631377236177871 0.0 54-55 9.579684936935338E-5 0.0 0.0 0.7797863538665364 0.0 56-57 9.579684936935338E-5 0.0 0.0 0.9204640271654319 0.0 58-59 9.579684936935338E-5 0.0 0.0 1.0747129207916524 0.0 60-61 9.579684936935338E-5 0.0 0.0 1.2460615520303024 0.0 62-63 9.579684936935338E-5 0.0 0.0 1.421848770623066 0.0 64-65 9.579684936935338E-5 0.0 0.0 1.609131611140152 0.0 66-67 9.579684936935338E-5 0.0 0.0 1.8278837166750703 0.0 68-69 9.579684936935338E-5 0.0 0.0 2.0763967100807346 0.0 70-71 9.579684936935338E-5 0.0 0.0 2.3543353023843516 0.0 72-73 9.579684936935338E-5 0.0 0.0 2.6325932175192 0.0 74-75 9.579684936935338E-5 0.0 0.0 2.924326556132004 0.0 76-77 9.579684936935338E-5 0.0 0.0 3.246810683392371 0.0 78-79 9.579684936935338E-5 0.0 0.0 3.622621723468344 0.0 80-81 1.2772913249247116E-4 0.0 0.0 4.039433815074401 0.0 82-83 1.2772913249247116E-4 0.0 0.0 4.462169345199795 0.0 84-85 1.4369527405403007E-4 0.0 0.0 4.894755984668672 0.0 86-87 1.5966141561558897E-4 0.0 0.0 5.350014545154963 0.0 88-89 1.5966141561558897E-4 0.0 0.0 5.859270596402445 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11290 0.0 26.488598 1 TACATGG 11120 0.0 26.027847 2 ACATGGG 11500 0.0 23.890871 3 AGTACAT 8485 0.0 19.483877 2 CATGGGG 6635 0.0 19.062162 4 GTATCAA 16095 0.0 19.052584 1 GAGTACA 9870 0.0 18.516375 1 GAGTACT 6295 0.0 18.467068 12-13 GTGTAGC 1775 0.0 17.91797 1 TACCGTA 185 1.5587154E-4 17.917704 7 TACCTGG 2735 0.0 17.839462 2 GTATAGC 1165 0.0 17.520838 1 AGAGTAC 12795 0.0 17.411959 10-11 AGTACTT 6720 0.0 17.369598 12-13 CATGGGA 5625 0.0 17.26367 4 ACCGTAT 220 2.99801E-5 17.219612 8 GTACTTT 6670 0.0 17.216797 14-15 CAACGCA 17735 0.0 17.014143 5 ATCAACG 17895 0.0 16.941431 3 TCAACGC 17865 0.0 16.91685 4 >>END_MODULE