FastQCFastQC Report
Fri 27 May 2016
SRR939169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939169_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3131627
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75860.24223829977197156No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56330.17987455083252252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47790.15260438104537993No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33710.10764372640803008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA1457.970739E-726.1781887
GTACATG116300.024.3985271
TACATGG115700.024.1955912
ACATGGG117350.022.7232693
CATGGGG65500.019.7035834
GAGTACT72950.019.54629912-13
TACCTGG25000.019.3587682
GTACTTT75800.018.56097614-15
GTGTAGC17650.018.0728721
AGTACAT88200.018.0216182
CATGGGA58650.017.7980424
AGTACTT75250.017.30936812-13
GAGTACA105550.017.140461
GTATCAA192400.016.9010121
AGAGTAC143400.016.26371210-11
GTACCTG29800.016.1362341
TATAGGG6600.015.81598952
GTATAGG9450.015.6180561
CCGTCCG1850.003167644615.3887759
ATCAACG210300.015.3647493
Produced by FastQC (version 0.11.5)