##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939169_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3131627 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51303427898661 34.0 31.0 34.0 31.0 34.0 2 32.71647006492152 34.0 31.0 34.0 31.0 34.0 3 32.8157976668358 34.0 31.0 34.0 31.0 34.0 4 36.26444975726675 37.0 37.0 37.0 35.0 37.0 5 36.22440922881301 37.0 37.0 37.0 35.0 37.0 6 36.25937635612415 37.0 37.0 37.0 35.0 37.0 7 36.21394118775959 37.0 37.0 37.0 35.0 37.0 8 36.19977283373786 37.0 37.0 37.0 35.0 37.0 9 37.94769843279548 39.0 38.0 39.0 35.0 39.0 10-11 37.889585669046795 39.0 38.0 39.0 35.0 39.0 12-13 37.82623776714149 39.0 38.0 39.0 35.0 39.0 14-15 39.21321488797996 41.0 39.0 41.0 36.0 41.0 16-17 39.15688665987361 40.0 39.0 41.0 36.0 41.0 18-19 39.16635250622121 40.5 38.5 41.0 36.0 41.0 20-21 39.228240144819296 41.0 39.0 41.0 36.0 41.0 22-23 39.19006446169994 41.0 39.0 41.0 36.0 41.0 24-25 39.14021130230388 41.0 39.0 41.0 36.0 41.0 26-27 39.01491876267512 40.0 39.0 41.0 35.0 41.0 28-29 38.87437951582356 40.0 38.0 41.0 35.0 41.0 30-31 38.720049833521045 40.0 38.0 41.0 35.0 41.0 32-33 38.618580693039114 40.0 38.0 41.0 34.5 41.0 34-35 38.592847903022935 40.0 38.0 41.0 35.0 41.0 36-37 38.50834997271386 40.0 38.0 41.0 34.0 41.0 38-39 38.39147590054627 40.0 38.0 41.0 34.0 41.0 40-41 38.248439389493065 40.0 38.0 41.0 34.0 41.0 42-43 38.11096101164027 40.0 38.0 41.0 33.0 41.0 44-45 37.9304837389638 40.0 38.0 41.0 33.0 41.0 46-47 37.696797862580695 40.0 37.5 41.0 33.0 41.0 48-49 37.437305911591636 40.0 37.0 41.0 32.5 41.0 50-51 37.206355354580865 40.0 37.0 41.0 32.0 41.0 52-53 36.9823166999135 40.0 36.0 41.0 31.0 41.0 54-55 36.74857685797191 39.0 36.0 41.0 31.0 41.0 56-57 36.472205661785395 39.0 35.0 41.0 31.0 41.0 58-59 36.17337681020122 39.0 35.0 40.0 30.0 41.0 60-61 35.80905500559294 38.0 35.0 40.0 29.5 41.0 62-63 35.41972080327574 38.0 34.5 40.0 29.0 41.0 64-65 35.00486169010549 37.0 34.0 40.0 28.0 41.0 66-67 34.642195893699984 36.5 34.0 39.0 28.0 41.0 68-69 34.16892050042997 36.0 34.0 39.0 27.5 40.0 70-71 33.72964756019794 35.5 33.0 38.5 26.0 40.0 72-73 33.26274984217469 35.0 33.0 37.5 26.0 40.0 74-75 32.77364433886922 35.0 32.5 37.0 25.5 39.0 76-77 30.785719531732227 33.5 30.0 35.5 22.5 37.5 78-79 31.775001780224784 34.5 31.5 36.0 24.0 38.0 80-81 31.815082064371012 35.0 32.0 36.0 25.0 37.0 82-83 31.539100921022843 35.0 32.0 35.0 24.0 37.0 84-85 31.174251116113126 34.5 31.5 35.0 22.5 36.5 86-87 30.638031285335067 34.0 31.0 35.0 19.5 36.0 88-89 30.1143190105335 34.0 30.5 35.0 17.5 36.0 90-91 29.68060867402152 34.0 30.0 35.0 9.5 35.0 92-93 29.411665246212273 34.0 30.0 35.0 7.0 35.0 94-95 29.098759526597515 34.0 29.5 35.0 2.0 35.0 96-97 28.559123739832362 34.0 29.0 35.0 2.0 35.0 98-99 27.80187311579572 34.0 29.0 35.0 2.0 35.0 100-101 24.15423691902005 30.0 14.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07082313652281869 1101 2 0.06251678450092868 1101 3 0.09000667237408777 1101 4 0.11281668459825056 1101 5 0.13248245727471897 1101 6 0.11540494446284555 1101 7 0.06707509488011709 1101 8 0.13408257990333539 1101 9 0.13294298238959357 1101 10-11 0.20859266137642152 1101 12-13 0.1815279224161941 1101 14-15 0.21564175726991408 1101 16-17 0.1870487101050884 1101 18-19 0.19415807098835103 1101 20-21 0.19755021498654912 1101 22-23 0.20085938082431198 1101 24-25 0.1811169755786537 1101 26-27 0.15561242115924045 1101 28-29 0.08128293733261671 1101 30-31 0.10824561489729234 1101 32-33 0.15827054288419617 1101 34-35 0.22073441914280068 1101 36-37 0.1627523704581364 1101 38-39 0.19708578109501929 1101 40-41 0.27170319034863866 1101 42-43 0.2230273428136087 1101 44-45 0.19607528062272905 1101 46-47 0.2068133131821952 1101 48-49 0.11191898758775665 1101 50-51 0.09371881547819783 1101 52-53 0.08923911763640291 1101 54-55 0.1632539617327211 1101 56-57 0.11128297654578745 1101 58-59 0.05997783794214229 1101 60-61 0.07672402609593121 1101 62-63 0.10560432638109063 1101 64-65 0.0023345816309685574 1101 66-67 0.03498078410981975 1101 68-69 0.02944034265021145 1101 70-71 0.04560924033696523 1101 72-73 -0.060061312553955304 1101 74-75 -0.030722560040665314 1101 76-77 -0.09939962551464632 1101 78-79 0.12442032976781192 1101 80-81 0.06798749804642412 1101 82-83 0.12621824063378995 1101 84-85 0.047572359258623464 1101 86-87 0.2125353989455263 1101 88-89 0.16554952983581472 1101 90-91 0.218442028123512 1101 92-93 -0.011084039927787614 1101 94-95 -0.09473591656802327 1101 96-97 -0.058602010266277205 1101 98-99 -0.09343131459626974 1101 100-101 -0.365749177660188 1102 1 0.1500180418745387 1102 2 0.09846234652659547 1102 3 0.15797808026795934 1102 4 0.10992128826227088 1102 5 0.08086031915079417 1102 6 0.09894111349325385 1102 7 0.11456387569047877 1102 8 0.05537607411954326 1102 9 0.18519540904080856 1102 10-11 0.1913466237669681 1102 12-13 0.15436143685764137 1102 14-15 0.2633906229659644 1102 16-17 0.26885126259862346 1102 18-19 0.3069148307722216 1102 20-21 0.27022946893601585 1102 22-23 0.25070683000006255 1102 24-25 0.27508226582369844 1102 26-27 0.2575482631180961 1102 28-29 0.09910965618139045 1102 30-31 0.11768277881969169 1102 32-33 0.13188390812414497 1102 34-35 0.17283181797085945 1102 36-37 0.24944834199634158 1102 38-39 0.26304516585155824 1102 40-41 0.35335214198576637 1102 42-43 0.3209676778330959 1102 44-45 0.20348716138645528 1102 46-47 0.15434260100192887 1102 48-49 0.19974609952549827 1102 50-51 0.20253620465672384 1102 52-53 0.21956702113683235 1102 54-55 0.19616822903105913 1102 56-57 0.2705348232561633 1102 58-59 0.24882908123404945 1102 60-61 0.22167928126450676 1102 62-63 0.06921909704868057 1102 64-65 0.0937329471347752 1102 66-67 0.18947181198898022 1102 68-69 0.10821772560393583 1102 70-71 0.09126012834108366 1102 72-73 0.13967943720130904 1102 74-75 0.14566836908162628 1102 76-77 0.31296590306076766 1102 78-79 0.26530563341018976 1102 80-81 0.2402534835742891 1102 82-83 0.2519544747123419 1102 84-85 0.23388319785198775 1102 86-87 0.30587107376079814 1102 88-89 0.27769128040633717 1102 90-91 0.41888670327587363 1102 92-93 0.3900625009432339 1102 94-95 0.2821984311143133 1102 96-97 0.1764449261706993 1102 98-99 0.07160035329632919 1102 100-101 -0.21126838086348698 1103 1 0.07307700993350608 1103 2 0.07297398426404555 1103 3 0.09376537782944894 1103 4 0.05380916440821437 1103 5 0.117714167133677 1103 6 0.1204865151999428 1103 7 0.10235931042139867 1103 8 0.13920041165355457 1103 9 0.13700209842814814 1103 10-11 0.1571725596735476 1103 12-13 0.15805196022557766 1103 14-15 0.154990448598042 1103 16-17 0.1540168460706539 1103 18-19 0.1852372000623319 1103 20-21 0.2419818004464176 1103 22-23 0.22573575922253752 1103 24-25 0.15506581975886036 1103 26-27 0.2692852713873606 1103 28-29 0.2288020024866384 1103 30-31 0.22130639510459815 1103 32-33 0.18802892410787564 1103 34-35 0.1719220665772383 1103 36-37 0.24821858584723344 1103 38-39 0.22200358174546153 1103 40-41 0.22045228465042754 1103 42-43 0.17819304836491767 1103 44-45 0.20306048837914403 1103 46-47 0.2503242791610276 1103 48-49 0.232012882437445 1103 50-51 0.236198062996003 1103 52-53 0.22821533954321893 1103 54-55 0.17677369520104236 1103 56-57 0.28575720839790364 1103 58-59 0.22964861044067675 1103 60-61 0.21404097580362702 1103 62-63 4.898692872004062E-4 1103 64-65 -0.012879361654952959 1103 66-67 0.05789820759279962 1103 68-69 0.09650660832829772 1103 70-71 -0.013572570927102845 1103 72-73 -0.020427165002068648 1103 74-75 -0.003002802089547174 1103 76-77 -0.0637582257601359 1103 78-79 -0.05324268763813578 1103 80-81 0.008921814661970728 1103 82-83 0.02196160794528268 1103 84-85 2.715706046529931E-5 1103 86-87 0.15575885743890439 1103 88-89 0.11729584831735451 1103 90-91 0.3507865209821439 1103 92-93 0.1683425331748758 1103 94-95 -0.11737383910312005 1103 96-97 -0.03699349025158938 1103 98-99 -0.09990106014056366 1103 100-101 -0.294179598532768 1104 1 0.09353667920051123 1104 2 0.12339213706751906 1104 3 0.12157238031534945 1104 4 0.015022106355097264 1104 5 0.10971630335599514 1104 6 0.09124264889185696 1104 7 0.07476618447964967 1104 8 0.05680949568466076 1104 9 0.08773852292603834 1104 10-11 0.10162454871269233 1104 12-13 0.04746093871712276 1104 14-15 0.05641370671855128 1104 16-17 0.10221930399788448 1104 18-19 0.14240428952300022 1104 20-21 0.08303783177767343 1104 22-23 0.05309695245529156 1104 24-25 0.12910156333588674 1104 26-27 0.06619734930861654 1104 28-29 0.12500721970103257 1104 30-31 0.17318201101691244 1104 32-33 0.15847632281215596 1104 34-35 0.18006463398585737 1104 36-37 0.23436378538693248 1104 38-39 0.24013654886925906 1104 40-41 0.27194706807181745 1104 42-43 0.3047575451128992 1104 44-45 0.3746833815616739 1104 46-47 0.38906091982427427 1104 48-49 0.4030094682964105 1104 50-51 0.3731666617994591 1104 52-53 0.4153387508868107 1104 54-55 0.4243480656194478 1104 56-57 0.4138752982591214 1104 58-59 0.41560008767655177 1104 60-61 0.4958214789602735 1104 62-63 0.4483186154645651 1104 64-65 0.3591910573879389 1104 66-67 0.38050789561533094 1104 68-69 0.24439600796082317 1104 70-71 0.3686476379903567 1104 72-73 0.2514839910375315 1104 74-75 0.2730942688699116 1104 76-77 0.2877973382724939 1104 78-79 0.4139190612667356 1104 80-81 0.42201138976989583 1104 82-83 0.41591037675643605 1104 84-85 0.4476720355310313 1104 86-87 0.5741295428738127 1104 88-89 0.5300462981320244 1104 90-91 0.5861026628120491 1104 92-93 0.8427537686870288 1104 94-95 0.5088446023446807 1104 96-97 0.5497319861069165 1104 98-99 0.39255381534183087 1104 100-101 0.027336292799638784 1105 1 0.01760324967564486 1105 2 0.024929476425157304 1105 3 0.006255482739021545 1105 4 0.03226596253601599 1105 5 0.030637865334959713 1105 6 0.03500059061875049 1105 7 0.04631648212360062 1105 8 0.044383277864525894 1105 9 0.06482789543831302 1105 10-11 0.01776317770762148 1105 12-13 0.08135769227702383 1105 14-15 0.06077449172273219 1105 16-17 0.09508063775816566 1105 18-19 0.14700837493837327 1105 20-21 0.0628659059364125 1105 22-23 0.05259680807125022 1105 24-25 -0.024964189688553517 1105 26-27 0.06245207081186521 1105 28-29 0.017239164803172002 1105 30-31 0.05394086645051743 1105 32-33 0.01953618874056673 1105 34-35 0.09772086743429753 1105 36-37 0.10252543553448135 1105 38-39 0.17260012757162002 1105 40-41 0.15117380809299164 1105 42-43 0.22801984569000666 1105 44-45 0.25367117445933474 1105 46-47 0.22293256942524664 1105 48-49 0.1520499311379595 1105 50-51 0.038304034629049966 1105 52-53 -0.09278750703193595 1105 54-55 -0.003876015510350328 1105 56-57 -0.03432275504737703 1105 58-59 -0.006587797317322952 1105 60-61 0.07281021797245302 1105 62-63 0.04234273654787302 1105 64-65 -0.007927617587377256 1105 66-67 -0.06110919597252007 1105 68-69 -0.11662431560453967 1105 70-71 -0.17290026050839202 1105 72-73 -0.19497637493853404 1105 74-75 -0.1566362916449009 1105 76-77 0.011598769343262205 1105 78-79 0.14277852891769172 1105 80-81 0.02402681685402186 1105 82-83 -0.0873426174590115 1105 84-85 0.015352599020587832 1105 86-87 -0.08435661101103875 1105 88-89 0.12202147181611167 1105 90-91 0.1910979153099852 1105 92-93 -0.012529284496114457 1105 94-95 -0.055394203538835285 1105 96-97 -0.09454660882595434 1105 98-99 -0.12305804165943712 1105 100-101 -0.36111554750435815 1106 1 0.10148592475051288 1106 2 0.13310008000565432 1106 3 0.1236047956621249 1106 4 0.0703565718493806 1106 5 0.1053865612816196 1106 6 0.08147401197159354 1106 7 0.07745980720823553 1106 8 0.009688235058746386 1106 9 0.060079442213755385 1106 10-11 0.07149855514625614 1106 12-13 0.08394534095875628 1106 14-15 0.10182423010298436 1106 16-17 0.05258543151951045 1106 18-19 0.10817538276442917 1106 20-21 0.20447090000128298 1106 22-23 0.15631230541460184 1106 24-25 0.19056921522852832 1106 26-27 0.1374064448373673 1106 28-29 0.18072792503650703 1106 30-31 0.1515973268457813 1106 32-33 0.18179007142720138 1106 34-35 0.14409714215872071 1106 36-37 0.1603644922933114 1106 38-39 0.2002090722275156 1106 40-41 0.11970847169750698 1106 42-43 0.15623871629254182 1106 44-45 0.2580412701727468 1106 46-47 0.18520084206457454 1106 48-49 0.19784488644819476 1106 50-51 0.23683036477501673 1106 52-53 0.295974633407063 1106 54-55 0.29665762634972737 1106 56-57 0.310220389960584 1106 58-59 0.2560694663724945 1106 60-61 0.238510234269512 1106 62-63 0.25399183668461234 1106 64-65 0.4092490736943901 1106 66-67 0.3428235376446054 1106 68-69 0.1505153894534459 1106 70-71 0.23654486522743667 1106 72-73 0.12776662744130363 1106 74-75 0.24543649724351013 1106 76-77 0.2836914498851577 1106 78-79 0.37997488068904417 1106 80-81 0.21701746511850573 1106 82-83 0.23819423180016486 1106 84-85 0.232829976743556 1106 86-87 0.3192789036233705 1106 88-89 0.5180481689347687 1106 90-91 0.5132658974918733 1106 92-93 0.5507587098912232 1106 94-95 0.40634401028083644 1106 96-97 0.5507974035089518 1106 98-99 0.6392463503390537 1106 100-101 0.03887237418193479 1107 1 -0.0014996265035946976 1107 2 -0.04020251449384915 1107 3 -0.035092184051372044 1107 4 0.00983262973400656 1107 5 0.03156349819230542 1107 6 0.07825371761830979 1107 7 -0.0011191846850024945 1107 8 0.0074276422063661585 1107 9 0.06180051811028875 1107 10-11 -0.04602818259696306 1107 12-13 -0.08170261162201342 1107 14-15 -0.05932130501603439 1107 16-17 -0.01918629062085131 1107 18-19 -0.023461432124676662 1107 20-21 0.05434120303837631 1107 22-23 0.007428315566258448 1107 24-25 -0.12235089928576315 1107 26-27 -0.08001944861503318 1107 28-29 0.005049046873217833 1107 30-31 -0.06742246664752116 1107 32-33 -0.05106807987284867 1107 34-35 -0.1277154779440295 1107 36-37 -0.13791891377863408 1107 38-39 -0.21264997546622766 1107 40-41 -0.11601085623131979 1107 42-43 -0.1332507874975235 1107 44-45 -0.2053514118932256 1107 46-47 -0.06456273409807523 1107 48-49 -0.15166853984862882 1107 50-51 -0.15744055901471654 1107 52-53 -0.15751121757868702 1107 54-55 -0.06294106939744637 1107 56-57 -0.15639118584351053 1107 58-59 -0.24929544989873875 1107 60-61 -0.21010735857726814 1107 62-63 -0.2707615149874485 1107 64-65 -0.14118231577186435 1107 66-67 -0.12620942163110982 1107 68-69 -0.25988529852872233 1107 70-71 -0.0831466632918918 1107 72-73 -0.13366771452169246 1107 74-75 -0.22455588341006916 1107 76-77 -0.16642171981897746 1107 78-79 0.05207322628243105 1107 80-81 -0.09103299064166848 1107 82-83 -0.12292301089282276 1107 84-85 0.030659583506842836 1107 86-87 -0.020995504896852424 1107 88-89 -0.002236940285200717 1107 90-91 0.09617307032218392 1107 92-93 0.028388925081738847 1107 94-95 -0.14168140248044736 1107 96-97 -0.08493368958713532 1107 98-99 0.05548788362046864 1107 100-101 -0.31740518292114217 1108 1 -0.08905763760062513 1108 2 -0.09671859491307089 1108 3 -0.061584417842510675 1108 4 -0.02850949445683426 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70-71 -0.09436222421675922 2316 72-73 -0.03466481173971658 2316 74-75 -0.2067306208173605 2316 76-77 -0.21605006305196994 2316 78-79 -0.23532567972112517 2316 80-81 -0.0823271615870027 2316 82-83 -0.2066884057047318 2316 84-85 -0.2874714779381726 2316 86-87 -0.41591941173936675 2316 88-89 -0.6439770154555085 2316 90-91 -0.704675531979909 2316 92-93 -0.6639656841333377 2316 94-95 -0.32420430593358773 2316 96-97 -0.5591652074233053 2316 98-99 -0.5118755346150401 2316 100-101 -0.1785011928543625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 18.0 5 54.0 6 100.0 7 240.0 8 538.0 9 1031.0 10 1794.0 11 2916.0 12 4230.0 13 6134.0 14 7651.0 15 10072.0 16 12756.0 17 18083.0 18 17695.0 19 13274.0 20 12623.0 21 13665.0 22 14801.0 23 16529.0 24 19005.0 25 22584.0 26 26983.0 27 33247.0 28 40689.0 29 50077.0 30 62375.0 31 76749.0 32 96457.0 33 126911.0 34 176928.0 35 262425.0 36 439668.0 37 773780.0 38 701851.0 39 67679.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.75654909118642 18.679401998678486 13.715359112906173 23.84868979722892 2 16.191359954426247 20.21105961853056 38.79826684340121 24.799313583641986 3 20.29491379401187 23.28920398246662 28.8036857518472 27.61219647167431 4 13.452049046709586 15.881552943565758 35.83437618847966 34.83202182124499 5 14.429815555939452 36.98754672890481 33.695871187724464 14.88676652743127 6 33.73304036527977 34.76193045978975 16.83754163570566 14.667487539224819 7 30.13941315488722 31.53702532261984 19.9253295491449 18.39823197334804 8 26.834645377626394 34.84323005262121 19.205064971019855 19.117059598732546 9 26.38356994622923 16.84986749699118 19.91986912873085 36.846693428048745 10-11 25.74631333808273 26.1182605719008 26.592423043995982 21.543003046020488 12-13 26.35981232758563 23.1035975868135 27.3648649727442 23.171725112856674 14-15 22.975187108945658 24.89845439931945 25.413926053088552 26.71243243864634 16-17 22.96166849615807 28.074387785650135 26.05651332202375 22.907430396168046 18-19 22.81055362631222 27.05989701832116 27.77549994012693 22.35404941523969 20-21 23.514134984785866 26.06512525278394 27.705135381704142 22.715604380726056 22-23 23.17087890735391 25.968833453026175 27.623420669192082 23.236866970427833 24-25 23.239917518399096 26.1438893603365 27.735292497543195 22.880900623721203 26-27 23.01785258675223 26.247935953322127 27.89730523558153 22.836906224344112 28-29 22.7197396113905 26.755421383197938 27.794657537439804 22.73018146797176 30-31 22.95286837607711 26.288236924594 27.612334936810402 23.14655976251849 32-33 22.862045831128675 26.302414048671825 27.74567341512894 23.08986670507056 34-35 23.366161556887164 26.312414382590998 27.493106296603052 22.828317763918783 36-37 23.276936366916143 26.08672321215963 27.68116365596972 22.95517676495451 38-39 22.986075989189892 26.408047248151007 27.80092741567798 22.804949346981125 40-41 23.171521945121945 26.3313039709734 27.58521741046635 22.911956673438304 42-43 22.893023955428017 26.36392073629612 27.373072063327164 23.369983244948695 44-45 23.05226686678216 26.30830026903825 27.422501816207756 23.216931047971833 46-47 23.30625807200488 26.594103710284216 27.084048161638556 23.015590056072345 48-49 23.307009221794715 26.499224199235982 27.13235042883788 23.06141615013142 50-51 23.03069860387157 26.706522445459218 27.24959521807292 23.013183732596293 52-53 23.31085563473886 26.79443323708397 26.814135484082136 23.080575644095035 54-55 22.954633486044155 26.483853281377378 27.079390361623528 23.48212287095494 56-57 23.123762823605748 26.69393577204437 26.9717466352155 23.210554769134383 58-59 23.40317840278961 26.42689387776413 26.891227258944937 23.278700460501327 60-61 23.151793843629452 26.53226638324807 27.335401062755864 22.980538710366613 62-63 22.97131413874557 26.577636147589327 27.269754521274812 23.18129519239029 64-65 22.800841307139535 26.65620966960349 27.10063736695709 23.44231165629988 66-67 22.943121800834813 26.619369557088163 26.82653990236569 23.61096873971134 68-69 22.757337402940088 26.56218340916744 27.137050489915953 23.543428697976516 70-71 22.9595227911119 26.45585289305733 27.03200716943735 23.552617146393416 72-73 22.92734735011545 26.321956605943175 27.111306678605086 23.639389365336292 74-75 22.67514617800907 26.854778682135517 26.77644879163451 23.69362634822091 76-77 22.87972696092824 26.44821194129916 26.884428020426725 23.78763307734587 78-79 22.86073799384119 26.42318499596773 27.034611413325056 23.681465596866023 80-81 22.844796960454193 26.574885906063844 26.94156081686197 23.638756316619993 82-83 22.870044510750564 26.46574294378949 26.97529029062752 23.688922254832427 84-85 22.759770362142483 26.46362840523926 26.87301630638264 23.903584926235617 86-87 22.682455500997392 26.443564267812388 27.016520894071917 23.857459337118307 88-89 22.64459556013972 26.42117161563533 26.846346822864557 24.087886001360395 90-91 22.64797538747196 26.29803547940669 26.946640477760536 24.107348655360813 92-93 22.624661238391415 26.318939005188035 27.0066326545275 24.049767101893043 94-95 22.546012025059177 26.154615476236476 27.0524874130923 24.246885085612046 96-97 22.38724471337104 26.087781207659788 27.220451222319898 24.304522856649275 98-99 22.273699625652107 26.25156510501571 26.836110473342682 24.6386247959895 100-101 22.423182939306347 25.530017281922703 27.368935839744335 24.67786393902662 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 211.0 1 155.0 2 140.5 3 198.0 4 171.5 5 149.5 6 321.0 7 686.5 8 1082.0 9 1318.0 10 1307.0 11 1251.5 12 1369.0 13 1578.5 14 1744.5 15 1930.0 16 2101.5 17 2233.5 18 2450.0 19 2710.0 20 3052.5 21 3628.0 22 4356.5 23 5417.0 24 6888.5 25 8916.5 26 11874.0 27 15569.5 28 18854.5 29 22498.5 30 27329.0 31 33229.5 32 39651.0 33 44627.5 34 49588.0 35 55627.0 36 62750.0 37 70171.0 38 76253.0 39 81008.5 40 85306.5 41 90537.5 42 96219.5 43 101812.5 44 107740.0 45 113535.5 46 116619.0 47 124188.5 48 140187.0 49 140626.0 50 128804.5 51 123685.0 52 121268.5 53 120654.5 54 119274.5 55 111841.0 56 100614.0 57 90616.5 58 82368.5 59 73113.0 60 62030.0 61 50943.5 62 40702.5 63 31877.5 64 24231.0 65 17694.5 66 12799.5 67 10165.0 68 7716.5 69 5387.0 70 3954.0 71 2863.5 72 2256.5 73 1858.5 74 1432.5 75 1010.5 76 615.5 77 336.5 78 185.5 79 102.5 80 63.5 81 42.0 82 21.5 83 8.5 84 5.5 85 5.5 86 4.0 87 2.0 88 3.0 89 2.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3501374844449866 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.26036210570416E-4 16-17 0.0020596322614410975 18-19 6.386456624623558E-5 20-21 0.0 22-23 0.0 24-25 6.226795209007969E-4 26-27 0.0011495621924322405 28-29 0.0 30-31 0.003161296029188661 32-33 0.0 34-35 0.010457822722821076 36-37 0.02789284930804339 38-39 0.02134673126780424 40-41 0.013730881742940649 42-43 0.010617484138436665 44-45 0.0032570928785580146 46-47 0.0015167834483480953 48-49 7.66374794954827E-4 50-51 7.983070780779449E-5 52-53 1.4369527405403007E-4 54-55 0.0 56-57 0.0 58-59 0.004741944043782992 60-61 0.010665382563121342 62-63 0.00491757160096014 64-65 0.002283158243302922 66-67 0.004662113335975197 68-69 0.003847840116335694 70-71 1.5966141561558895E-5 72-73 0.0 74-75 0.0 76-77 0.2171235590956394 78-79 4.949503884083258E-4 80-81 0.0034007881526120446 82-83 7.66374794954827E-4 84-85 1.5966141561558895E-5 86-87 0.11070922558784937 88-89 0.40482151929332577 90-91 0.39085114542696175 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.001532749589909654 100-101 9.899007768166515E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3131627.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.55364769144053 #Duplication Level Percentage of deduplicated Percentage of total 1 73.95367815680827 38.865355473390835 2 14.16224490380777 14.88555258389225 3 4.910114049761891 7.741332116879361 4 2.268748042027471 4.769239412054289 5 1.2663314665552892 3.3275168876965937 6 0.7626136763497761 2.404687828293623 7 0.5158307956888009 1.8976152933517276 8 0.3556775130688279 1.4953720570869533 9 0.25420828838777804 1.2023615545357862 >10 1.405698824907188 13.500470808101802 >50 0.09692399365318641 3.512480707525592 >100 0.044949022301208875 4.212922525866156 >500 0.0017035809616683048 0.6274092009157358 >1k 0.0011560013665691776 1.1350088880219025 >5k 1.2168435437570292E-4 0.422674662387287 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7586 0.24223829977197156 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5633 0.17987455083252252 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4779 0.15260438104537993 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3371 0.10764372640803008 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.193228312311779E-5 0.0 10-11 0.0 0.0 0.0 4.789842468467669E-5 0.0 12-13 0.0 0.0 0.0 6.386456624623558E-5 0.0 14-15 0.0 0.0 0.0 1.1176299093091227E-4 0.0 16-17 0.0 0.0 0.0 1.5966141561558894E-4 0.0 18-19 0.0 0.0 0.0 2.3949212342338344E-4 0.0 20-21 0.0 0.0 0.0 2.8739054810806013E-4 0.0 22-23 3.193228312311779E-5 0.0 0.0 5.428488130930025E-4 0.0 24-25 3.193228312311779E-5 0.0 0.0 6.705779455854736E-4 0.0 26-27 3.193228312311779E-5 1.5966141561558895E-5 0.0 9.26036210570416E-4 0.0 28-29 3.193228312311779E-5 3.193228312311779E-5 0.0 0.0023949212342338345 0.0 30-31 3.193228312311779E-5 3.193228312311779E-5 0.0 0.007392323543001769 0.0 32-33 3.193228312311779E-5 3.193228312311779E-5 0.0 0.016844279347444636 0.0 34-35 3.193228312311779E-5 3.193228312311779E-5 0.0 0.03485408702888307 0.0 36-37 3.193228312311779E-5 3.193228312311779E-5 0.0 0.06710569298323205 0.0 38-39 3.193228312311779E-5 3.193228312311779E-5 0.0 0.11387052161703803 0.0 40-41 3.193228312311779E-5 3.193228312311779E-5 0.0 0.16470671634904155 0.0 42-43 3.193228312311779E-5 3.193228312311779E-5 0.0 0.21832101971275636 0.0 44-45 3.193228312311779E-5 3.193228312311779E-5 0.0 0.2873905481080601 0.0 46-47 6.386456624623558E-5 3.193228312311779E-5 0.0 0.37225059050774567 0.0 48-49 6.386456624623558E-5 3.193228312311779E-5 0.0 0.4680634060186606 0.0 50-51 6.386456624623558E-5 3.193228312311779E-5 0.0 0.5674207049562416 0.0 52-53 6.386456624623558E-5 3.193228312311779E-5 0.0 0.6685662117487172 1.5966141561558895E-5 54-55 6.386456624623558E-5 4.789842468467669E-5 0.0 0.7857097923858749 3.193228312311779E-5 56-57 6.386456624623558E-5 6.386456624623558E-5 0.0 0.9267546869406862 3.193228312311779E-5 58-59 6.386456624623558E-5 6.386456624623558E-5 0.0 1.0820733120515311 3.193228312311779E-5 60-61 6.386456624623558E-5 6.386456624623558E-5 0.0 1.2542362165098206 3.193228312311779E-5 62-63 6.386456624623558E-5 6.386456624623558E-5 0.0 1.4304385547831846 3.193228312311779E-5 64-65 6.386456624623558E-5 6.386456624623558E-5 0.0 1.6192062464654953 3.193228312311779E-5 66-67 9.579684936935338E-5 6.386456624623558E-5 0.0 1.8381978441238371 3.193228312311779E-5 68-69 9.579684936935338E-5 6.386456624623558E-5 0.0 2.088275519402534 3.193228312311779E-5 70-71 9.579684936935338E-5 6.386456624623558E-5 0.0 2.3671561140582833 3.193228312311779E-5 72-73 9.579684936935338E-5 6.386456624623558E-5 0.0 2.6466913205180562 3.193228312311779E-5 74-75 9.579684936935338E-5 6.386456624623558E-5 0.0 2.94046832525074 3.193228312311779E-5 76-77 9.579684936935338E-5 6.386456624623558E-5 0.0 3.2650919154803555 3.193228312311779E-5 78-79 9.579684936935338E-5 6.386456624623558E-5 0.0 3.6426911634112233 3.193228312311779E-5 80-81 9.579684936935338E-5 6.386456624623558E-5 0.0 4.060062069971934 3.193228312311779E-5 82-83 1.2772913249247116E-4 6.386456624623558E-5 0.0 4.483340448910423 3.193228312311779E-5 84-85 1.2772913249247116E-4 6.386456624623558E-5 0.0 4.916709429315816 3.193228312311779E-5 86-87 1.2772913249247116E-4 6.386456624623558E-5 0.0 5.372750330738622 3.193228312311779E-5 88-89 1.2772913249247116E-4 6.386456624623558E-5 0.0 5.883108045753852 3.193228312311779E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCGTA 145 7.970739E-7 26.178188 7 GTACATG 11630 0.0 24.398527 1 TACATGG 11570 0.0 24.195591 2 ACATGGG 11735 0.0 22.723269 3 CATGGGG 6550 0.0 19.703583 4 GAGTACT 7295 0.0 19.546299 12-13 TACCTGG 2500 0.0 19.358768 2 GTACTTT 7580 0.0 18.560976 14-15 GTGTAGC 1765 0.0 18.072872 1 AGTACAT 8820 0.0 18.021618 2 CATGGGA 5865 0.0 17.798042 4 AGTACTT 7525 0.0 17.309368 12-13 GAGTACA 10555 0.0 17.14046 1 GTATCAA 19240 0.0 16.901012 1 AGAGTAC 14340 0.0 16.263712 10-11 GTACCTG 2980 0.0 16.136234 1 TATAGGG 660 0.0 15.8159895 2 GTATAGG 945 0.0 15.618056 1 CCGTCCG 185 0.0031676446 15.388775 9 ATCAACG 21030 0.0 15.364749 3 >>END_MODULE