FastQCFastQC Report
Fri 27 May 2016
SRR939168_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939168_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3084628
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69960.2268020649491608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51890.16822125714997077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43010.13943334496088344No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35070.11369280185487521No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG116350.024.9378931
TACATGG116500.024.1085112
ACATGGG118100.022.6990763
GTATCAA171100.020.0665721
CATGGGG67550.019.983024
GAGTACT65650.019.57913812-13
AGTACAT87900.019.2362752
GTATAGG8200.018.5319461
GAGTACA102550.018.1987611
ATCAACG187300.018.0551913
TCAACGC187750.018.0371444
CAACGCA187350.018.02485
GTACTTT70400.017.95591714-15
AGAGTAC131600.017.77085710-11
AGTACTT70550.017.74953812-13
TACCTGG24350.017.7004412
AACGCAG191450.017.6799476
TATCAAC194050.017.5491852
GTGTAGC17900.016.7136941
GGACCGA5200.016.3869976
Produced by FastQC (version 0.11.5)