##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939168_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3084628 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17027045076424 33.0 31.0 34.0 30.0 34.0 2 32.267020853081796 34.0 31.0 34.0 30.0 34.0 3 32.341665186207216 34.0 31.0 34.0 30.0 34.0 4 35.76135987872768 37.0 35.0 37.0 35.0 37.0 5 35.797101952001995 37.0 35.0 37.0 35.0 37.0 6 35.877725288106056 37.0 37.0 37.0 35.0 37.0 7 35.81075416549419 37.0 36.0 37.0 35.0 37.0 8 35.80481860373439 37.0 36.0 37.0 35.0 37.0 9 37.49700968804018 39.0 38.0 39.0 35.0 39.0 10-11 37.403960704499866 39.0 38.0 39.0 35.0 39.0 12-13 37.31410286750947 39.0 37.0 39.0 34.0 39.0 14-15 38.639666274182815 40.0 38.0 41.0 34.0 41.0 16-17 38.55321306167227 40.0 38.0 41.0 34.0 41.0 18-19 38.42987809226915 40.0 38.0 41.0 34.0 41.0 20-21 38.40154501612512 40.0 38.0 41.0 34.0 41.0 22-23 38.454701669050536 40.0 38.0 41.0 34.0 41.0 24-25 38.40689169001902 40.0 38.0 41.0 34.0 41.0 26-27 38.30363337167399 40.0 38.0 41.0 34.0 41.0 28-29 38.17513862287446 40.0 38.0 41.0 34.0 41.0 30-31 38.00353057159567 40.0 38.0 41.0 33.0 41.0 32-33 37.839303637261935 40.0 38.0 41.0 33.0 41.0 34-35 37.55002240140465 40.0 38.0 41.0 32.5 41.0 36-37 37.309676726010395 40.0 37.0 41.0 31.5 41.0 38-39 37.13976336854881 40.0 37.0 41.0 31.0 41.0 40-41 36.974245840989575 40.0 37.0 41.0 31.0 41.0 42-43 36.790062853608276 40.0 37.0 41.0 30.0 41.0 44-45 36.55900209036552 40.0 36.0 41.0 30.0 41.0 46-47 36.500951978650264 40.0 36.0 41.0 30.0 41.0 48-49 36.42749190502064 40.0 36.0 41.0 30.0 41.0 50-51 35.31191686647466 38.5 34.5 40.0 28.0 40.5 52-53 35.459664179927046 38.5 35.0 39.5 28.0 40.5 54-55 35.99946946601016 39.0 35.0 41.0 29.0 41.0 56-57 35.90490279541001 39.0 35.0 41.0 28.0 41.0 58-59 35.699522276268 39.0 35.0 41.0 28.0 41.0 60-61 35.399833950803796 39.0 35.0 41.0 28.0 41.0 62-63 35.01981843515652 38.0 35.0 40.0 27.0 41.0 64-65 34.63664613690857 37.5 34.0 40.0 26.0 41.0 66-67 34.24418001133362 37.0 34.0 40.0 26.0 41.0 68-69 33.833034810032196 36.0 34.0 39.0 26.0 41.0 70-71 33.40116847153044 36.0 33.5 39.0 25.0 40.5 72-73 32.95378713413741 35.0 33.0 38.5 24.0 40.0 74-75 32.519139747159144 35.0 33.0 37.0 22.5 39.5 76-77 32.082091746557445 35.0 32.0 37.0 21.5 39.0 78-79 31.65880991160036 35.0 32.0 36.0 20.5 39.0 80-81 31.22023158059902 35.0 31.5 36.0 19.0 37.5 82-83 30.846592036381697 35.0 31.0 35.5 17.5 37.0 84-85 30.481128356482532 34.5 31.0 35.0 13.0 37.0 86-87 30.087258333906064 34.0 31.0 35.0 7.0 36.0 88-89 29.682601435246 34.0 30.0 35.0 3.5 36.0 90-91 29.288436077219036 34.0 30.0 35.0 2.0 35.5 92-93 28.890652454688215 34.0 29.0 35.0 2.0 35.0 94-95 28.424312753434123 34.0 29.0 35.0 2.0 35.0 96-97 27.843084644242353 34.0 29.0 35.0 2.0 35.0 98-99 27.148680975469325 34.0 27.0 35.0 2.0 35.0 100-101 24.21188373444059 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22115708144531254 1101 2 0.302154785434567 1101 3 0.22479472276234702 1101 4 0.22792792746551527 1101 5 0.08031166657323041 1101 6 0.145789527531349 1101 7 0.14166446570065716 1101 8 0.18424851992971014 1101 9 0.2244849753819409 1101 10-11 0.1947735265592101 1101 12-13 0.22229627891605475 1101 14-15 0.2768370832436631 1101 16-17 0.36299548713221697 1101 18-19 0.4349616325075729 1101 20-21 0.4869449549509639 1101 22-23 0.43524521775970726 1101 24-25 0.3450506220181069 1101 26-27 0.3575213496406491 1101 28-29 0.35210363643719234 1101 30-31 0.4345317152434447 1101 32-33 0.33268909741879327 1101 34-35 0.36191955364259343 1101 36-37 0.40988457549579493 1101 38-39 0.32388621582042276 1101 40-41 0.31746006101819546 1101 42-43 0.29715845477279146 1101 44-45 0.27261611693928245 1101 46-47 0.40543675655846556 1101 48-49 0.34978278430967435 1101 50-51 0.3621335387648159 1101 52-53 0.32509144986983074 1101 54-55 0.2546730666591799 1101 56-57 0.17042355448763402 1101 58-59 0.1633588736863274 1101 60-61 0.09568784914414863 1101 62-63 0.03827858674329576 1101 64-65 0.015068680675966561 1101 66-67 0.1160785060607239 1101 68-69 0.024783883233752135 1101 70-71 0.013555334143518394 1101 72-73 -0.09795399016094564 1101 74-75 -0.15795062166630203 1101 76-77 -0.13150693165293603 1101 78-79 -0.15625258337392012 1101 80-81 -0.237575649110493 1101 82-83 -0.3304028254351934 1101 84-85 -0.21552546904689152 1101 86-87 -0.3353260846223556 1101 88-89 -0.324216519453671 1101 90-91 -0.2541036527605307 1101 92-93 -0.40087798509689065 1101 94-95 -0.5610492889420833 1101 96-97 -0.6248096100522496 1101 98-99 -0.8237162527422939 1101 100-101 -1.3235164578247627 1102 1 0.187385393430759 1102 2 0.2289746269316666 1102 3 0.2662913969937293 1102 4 0.15174610073599126 1102 5 0.2417904476138517 1102 6 0.23577788840965042 1102 7 0.28030478264947334 1102 8 0.24655001734474524 1102 9 0.2607665538006998 1102 10-11 0.339115659752359 1102 12-13 0.3605415495434485 1102 14-15 0.3251915080331429 1102 16-17 0.3053866072089235 1102 18-19 0.30918069302958173 1102 20-21 0.38702786766609876 1102 22-23 0.39203173746287234 1102 24-25 0.4521227062186739 1102 26-27 0.48729392376703373 1102 28-29 0.5443723819051272 1102 30-31 0.5924114861080341 1102 32-33 0.5635772179641947 1102 34-35 0.47934917399638266 1102 36-37 0.3957715349782589 1102 38-39 0.26734394671880324 1102 40-41 0.4587243802008061 1102 42-43 0.40916527014098847 1102 44-45 0.49280514938208597 1102 46-47 0.5117001568630499 1102 48-49 0.45937252307764 1102 50-51 0.402905866432441 1102 52-53 0.43618807737377807 1102 54-55 0.4654294955999916 1102 56-57 0.46139746547471105 1102 58-59 0.4608664348415843 1102 60-61 0.3691413204374001 1102 62-63 0.35788010709465823 1102 64-65 0.4148111811353985 1102 66-67 0.4811273775502869 1102 68-69 0.40793002188033256 1102 70-71 0.31150274582794424 1102 72-73 0.24455508821151284 1102 74-75 0.3127651629486792 1102 76-77 0.2628860603706329 1102 78-79 0.17191430482070658 1102 80-81 0.1415648224092756 1102 82-83 -0.04757437640157747 1102 84-85 -0.0563496527769054 1102 86-87 -0.11057834869508198 1102 88-89 -0.15357702308430987 1102 90-91 -0.28555086428625387 1102 92-93 -0.39209073314053455 1102 94-95 -0.48166926805719257 1102 96-97 -0.5791596071317855 1102 98-99 -0.7981530659878118 1102 100-101 -1.0846205645684748 1103 1 0.10192451503354505 1103 2 0.15345664415958282 1103 3 0.3378460215096979 1103 4 0.21877515524320756 1103 5 0.24007593636824254 1103 6 0.15478406804692924 1103 7 0.14476869428535366 1103 8 0.115214647132305 1103 9 0.23828967569053106 1103 10-11 0.23728522855774514 1103 12-13 0.26633409699471855 1103 14-15 0.2692210786601308 1103 16-17 0.3394110442381688 1103 18-19 0.36572727214908696 1103 20-21 0.3980141808321278 1103 22-23 0.3945052483745286 1103 24-25 0.36471837467657764 1103 26-27 0.38786883151407636 1103 28-29 0.34933472172169644 1103 30-31 0.4262711512097752 1103 32-33 0.3554397755446672 1103 34-35 0.42461301608029345 1103 36-37 0.3458898009262228 1103 38-39 0.35503214413599693 1103 40-41 0.25806556849067874 1103 42-43 0.35102051372467713 1103 44-45 0.39869139879214543 1103 46-47 0.4625748004782082 1103 48-49 0.39241081254078836 1103 50-51 0.4299371565386778 1103 52-53 0.48859217431597557 1103 54-55 0.46838131430119745 1103 56-57 0.5440151311229329 1103 58-59 0.4249414626485688 1103 60-61 0.46934445634801847 1103 62-63 0.25525258825076236 1103 64-65 0.35111358316235197 1103 66-67 0.34015008958029824 1103 68-69 0.2966310008519031 1103 70-71 0.3544495689313436 1103 72-73 0.3212898164482283 1103 74-75 0.14086084658658393 1103 76-77 0.12628005847727053 1103 78-79 0.1727144284686517 1103 80-81 0.18305657415290355 1103 82-83 0.012511211160759927 1103 84-85 0.018306796374254475 1103 86-87 -0.03399335039248186 1103 88-89 -0.07547347344986832 1103 90-91 0.05536266771179399 1103 92-93 0.05854889657803142 1103 94-95 -0.004780117888419255 1103 96-97 -0.15702496887102768 1103 98-99 -0.1013636345751614 1103 100-101 -0.15163512908206656 1104 1 0.12184624761240315 1104 2 0.2880251812848087 1104 3 0.19173844615120572 1104 4 0.2028593414583142 1104 5 0.2866588741876157 1104 6 0.2615009297651696 1104 7 0.24464128468185464 1104 8 0.19248681391317746 1104 9 0.22998807713145197 1104 10-11 0.3299847035524195 1104 12-13 0.29395839792641 1104 14-15 0.4679851202823784 1104 16-17 0.3153514956953458 1104 18-19 0.441407418116178 1104 20-21 0.47977464651635415 1104 22-23 0.5157723454037111 1104 24-25 0.33085262775242796 1104 26-27 0.4268621225355602 1104 28-29 0.4679052173967264 1104 30-31 0.47126146898541776 1104 32-33 0.44975730456537377 1104 34-35 0.46798774333078796 1104 36-37 0.41887034938941525 1104 38-39 0.18164742172993442 1104 40-41 0.27564098291145456 1104 42-43 0.27005356165588523 1104 44-45 0.3526698062733047 1104 46-47 0.45704510650361385 1104 48-49 0.5103285470574193 1104 50-51 0.34607292766372666 1104 52-53 0.4536815701030932 1104 54-55 0.4153103140745529 1104 56-57 0.5585100375351999 1104 58-59 0.4296253768399083 1104 60-61 0.4707058402123323 1104 62-63 0.3355264310112318 1104 64-65 0.3036274060656652 1104 66-67 0.24939544867749674 1104 68-69 0.19426854343385003 1104 70-71 0.18912369037368393 1104 72-73 0.21760212870616158 1104 74-75 0.24002321633064128 1104 76-77 0.15597569760129204 1104 78-79 0.24712407378045143 1104 80-81 0.20364005633939897 1104 82-83 0.015740927426712403 1104 84-85 -0.025750320626592327 1104 86-87 -0.053956363256748574 1104 88-89 -0.1286288985182935 1104 90-91 0.13339412541987272 1104 92-93 0.09250439483240669 1104 94-95 -0.07931453619194073 1104 96-97 -0.007845740791054823 1104 98-99 0.024512539482763884 1104 100-101 -0.26028357525217416 1105 1 0.22814791683899927 1105 2 0.272237575944267 1105 3 0.24596187281940018 1105 4 0.24680244011290853 1105 5 0.2961925266911436 1105 6 0.31450565058193547 1105 7 0.2678486730989107 1105 8 0.25918195469578365 1105 9 0.3111416176413542 1105 10-11 0.3245423015211699 1105 12-13 0.2589940786973486 1105 14-15 0.2572441712524096 1105 16-17 0.25759137078569694 1105 18-19 0.2178861641736134 1105 20-21 0.2484549456793843 1105 22-23 0.2367188650590606 1105 24-25 0.4474058129176868 1105 26-27 0.3760578357094957 1105 28-29 0.3146985810454055 1105 30-31 0.46939599942944454 1105 32-33 0.4269858832689266 1105 34-35 0.4362200243538936 1105 36-37 0.47611326867761505 1105 38-39 0.378348612406576 1105 40-41 0.3688334147173222 1105 42-43 0.3736497834624046 1105 44-45 0.24256295390200933 1105 46-47 0.26866823121613237 1105 48-49 0.44606241178210126 1105 50-51 0.4281995334395887 1105 52-53 0.3871405001265842 1105 54-55 0.4056706536694463 1105 56-57 0.3679521715949008 1105 58-59 0.3992457068453277 1105 60-61 0.41029327183338893 1105 62-63 0.4120386606782489 1105 64-65 0.35230479707003326 1105 66-67 0.33848142185569685 1105 68-69 0.3695085884448588 1105 70-71 0.23486616406934502 1105 72-73 0.18348099581280763 1105 74-75 0.22271113779547136 1105 76-77 0.15034482994843046 1105 78-79 0.06679295437807653 1105 80-81 0.11757736177768052 1105 82-83 0.21543209278466335 1105 84-85 0.07274359713797196 1105 86-87 0.03732927387897078 1105 88-89 0.18121493618535567 1105 90-91 0.2113656321386692 1105 92-93 0.17566534768173625 1105 94-95 0.05587967644399683 1105 96-97 0.17282836422504033 1105 98-99 0.26453765796519946 1105 100-101 0.15804214929771732 1106 1 0.15038887363983378 1106 2 0.22286593732525262 1106 3 0.32356097934265193 1106 4 0.32352053952304516 1106 5 0.31716049598330187 1106 6 0.2985716203945685 1106 7 0.26144849527101144 1106 8 0.27514309279432325 1106 9 0.276503128744757 1106 10-11 0.39546879570923466 1106 12-13 0.36566777534897454 1106 14-15 0.3222611408048124 1106 16-17 0.3287808117121358 1106 18-19 0.37462681736582937 1106 20-21 0.40550598445453545 1106 22-23 0.441722443303334 1106 24-25 0.5021380409711256 1106 26-27 0.4805276591827763 1106 28-29 0.3727867685020314 1106 30-31 0.4347886846306892 1106 32-33 0.3702327604375597 1106 34-35 0.29196798583897277 1106 36-37 0.2939928336498596 1106 38-39 0.3742376976772235 1106 40-41 0.5301973221600633 1106 42-43 0.348801316685865 1106 44-45 0.32197964586939776 1106 46-47 0.23806475489760714 1106 48-49 0.35594351648369127 1106 50-51 0.41366969305097 1106 52-53 0.3883806870627424 1106 54-55 0.4053101184508563 1106 56-57 0.43726303427705915 1106 58-59 0.44324862365656514 1106 60-61 0.5651201064968134 1106 62-63 0.47141718470670213 1106 64-65 0.36499129949890374 1106 66-67 0.26830310601600615 1106 68-69 0.32949731155380846 1106 70-71 0.3183653074593522 1106 72-73 0.39814718899462065 1106 74-75 0.35720839447485986 1106 76-77 0.3896073863328908 1106 78-79 0.29326599827120603 1106 80-81 0.30017489604207626 1106 82-83 0.38371800534538636 1106 84-85 0.28775270539612663 1106 86-87 0.3173485747117262 1106 88-89 0.2788427228786219 1106 90-91 0.23923256482430588 1106 92-93 0.08166188871226865 1106 94-95 0.267690051183763 1106 96-97 0.20574956694884605 1106 98-99 0.2283621505051059 1106 100-101 -0.03508138687507767 1107 1 0.007503653140368272 1107 2 0.08601890377739352 1107 3 0.10431920469984846 1107 4 0.06838070132088347 1107 5 0.13936753447445938 1107 6 0.14295171222577352 1107 7 0.1513568066605515 1107 8 0.17272491858120986 1107 9 0.2029321686998884 1107 10-11 0.05922814756724648 1107 12-13 0.11442731733370692 1107 14-15 0.09615246437196845 1107 16-17 0.1530483485254237 1107 18-19 0.2399314241362518 1107 20-21 0.16359397510030504 1107 22-23 0.2522300341126069 1107 24-25 0.21388454501606446 1107 26-27 0.27554170479417905 1107 28-29 0.3383009899806453 1107 30-31 0.2623662088005574 1107 32-33 0.25302106384177137 1107 34-35 0.26170064390557 1107 36-37 0.26780798770003145 1107 38-39 0.3417558547789312 1107 40-41 0.35319041580697785 1107 42-43 0.1768939654362498 1107 44-45 0.16262006395805173 1107 46-47 0.3455843854813949 1107 48-49 0.270459691514354 1107 50-51 0.1109672793922698 1107 52-53 0.16825672444165463 1107 54-55 0.13679320192598254 1107 56-57 0.11521974242304367 1107 58-59 0.1714899277357418 1107 60-61 0.11800361585580532 1107 62-63 0.0807259077205913 1107 64-65 0.0920853076553314 1107 66-67 0.07348346497525426 1107 68-69 0.06070566191932869 1107 70-71 0.0688067579360947 1107 72-73 0.1309616348227678 1107 74-75 0.15271629424005084 1107 76-77 0.0663716434602648 1107 78-79 -0.015563248922045858 1107 80-81 -0.019727568950838048 1107 82-83 0.06955724845091638 1107 84-85 0.09999686612925629 1107 86-87 0.01978374150039386 1107 88-89 0.05172542419905568 1107 90-91 0.07175635443007877 1107 92-93 -0.03728851673296063 1107 94-95 -0.06308825630818049 1107 96-97 -0.1596518856192901 1107 98-99 -0.24584430809955649 1107 100-101 -0.14497534369730403 1108 1 0.07897661011561041 1108 2 0.20274727598521736 1108 3 0.19568408556244776 1108 4 0.24406426159347916 1108 5 0.20985251717404907 1108 6 0.2770749907554446 1108 7 0.14783673407655584 1108 8 0.29740199291581604 1108 9 0.3187811226766968 1108 10-11 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8 4594.0 9 5633.0 10 6834.0 11 8409.0 12 10775.0 13 17058.0 14 13590.0 15 12873.0 16 14903.0 17 19734.0 18 17561.0 19 13839.0 20 14155.0 21 15524.0 22 16865.0 23 18759.0 24 21241.0 25 24407.0 26 28373.0 27 33094.0 28 39231.0 29 47033.0 30 57010.0 31 70962.0 32 89689.0 33 118321.0 34 164878.0 35 249150.0 36 416425.0 37 694528.0 38 678950.0 39 116140.0 40 35.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.46345036947649 18.14636254583064 13.325073566598519 24.065113518094357 2 15.873754629417766 19.755438866045395 39.417956704578316 24.95284979995852 3 20.255261473589098 23.167036450902216 28.60994016376097 27.96776191174771 4 13.186554574931403 15.439003781943727 36.08106103468345 35.29338060844142 5 14.45547929086279 36.8036239164201 33.70375359705516 15.037143195661947 6 33.75135281034183 34.41990713946469 17.120025745570437 14.708714304623044 7 30.198633039232796 31.3957781637978 20.0130687315349 18.392520065434503 8 26.87778671422486 34.49830140815551 19.448884730322792 19.175027147296834 9 26.508667257136736 16.84328859387014 19.99617167755143 36.651872471441685 10-11 25.772229112723664 25.866929799830846 26.766780984465388 21.5940601029801 12-13 26.39781261380808 22.993283244936478 27.426374031034474 23.18253011022097 14-15 23.079241448714345 24.70626803382557 25.488385356614746 26.726105160845343 16-17 23.036737710393414 27.894199572696227 26.11957984661381 22.94948287029655 18-19 22.9452352585417 26.949412989251332 27.69307241659464 22.412279335612332 20-21 23.60556497283751 26.0036381044804 27.61951715056571 22.771279772116376 22-23 23.252539297149482 25.914775663307978 27.544192815417574 23.288492224124965 24-25 23.256411317377758 26.04580596115199 27.68342302565338 23.014359695816875 26-27 23.069215386302965 26.182229927388683 27.80188346750665 22.946671218801704 28-29 22.814169679380996 26.624070853264627 27.748612009611477 22.8131474577429 30-31 22.974640324624094 26.246969547265813 27.556576157469486 23.221813970640607 32-33 22.92029978333118 26.241035337864638 27.684645067924983 23.15401981087919 34-35 23.426206228930628 26.26055166197469 27.41326700972393 22.89997509937075 36-37 23.314046483979336 26.055383045040887 27.543546955232866 23.087023515746907 38-39 23.04412094619572 26.342003356484856 27.69911350155076 22.914762195768663 40-41 23.21121698144092 26.27635495011068 27.509250872723072 23.00317719572532 42-43 22.965737938189545 26.288782236866332 27.275842035241222 23.469637789702908 44-45 23.11164742681162 26.264964324161568 27.32874644369953 23.294641805327284 46-47 23.32338140174944 26.577995412465853 26.994418902022353 23.10420428376235 48-49 23.297384863709645 26.389047758247436 27.157111856086196 23.156455521956723 50-51 23.062999149122547 26.659854650187043 27.241944771503068 23.035201429187346 52-53 23.289862208733744 26.72778411973824 26.817531097784126 23.164822573743894 54-55 22.960645973103787 26.46092694644301 27.03199772288583 23.546429357567373 56-57 23.142563952209922 26.6625782859749 26.968245824069324 23.226611937745854 58-59 23.437302778170015 26.33317681808906 26.94140385216427 23.28811655157666 60-61 23.187317977295255 26.406365754475615 27.389856068275392 23.016460199953737 62-63 23.00450998231683 26.529789748648103 27.351018676067284 23.114681592967784 64-65 22.849558295048546 26.56944823300095 27.15884985616521 23.42214361578529 66-67 22.924251765218557 26.567233938739697 26.90480392698489 23.603710369056856 68-69 22.8103914570562 26.47266828777491 27.20537791984071 23.51156233532818 70-71 22.98030141135407 26.35113242763461 27.120681888263835 23.54788427274748 72-73 22.969689108109968 26.213050034922336 27.25773791268406 23.55952294428364 74-75 22.756624410632952 26.759089110537325 26.941774153700536 23.542512325129184 76-77 22.970558553614936 26.412285826845803 26.93407958780476 23.683076031734497 78-79 22.972900334632946 26.352132763797094 27.123218678981715 23.551748222588245 80-81 22.983901576546273 26.498331449705177 27.07172762639069 23.446039347357868 82-83 22.994961081116834 26.377320515090936 27.106529252171264 23.52118915162097 84-85 22.990709534310273 26.31307572605996 27.035828602409513 23.66038613722025 86-87 22.917351572435038 26.298442862323434 27.190332720027033 23.5938728452145 88-89 22.91653394798579 26.300212670315066 26.99383104562986 23.78942233606928 90-91 22.932679897897724 26.15674954437251 27.17293246805393 23.737638089675833 92-93 22.960825908917496 26.144178138393425 27.212335766934864 23.682660185754216 94-95 22.93304196345492 26.005266820173617 27.266288928759412 23.795402287612056 96-97 22.8775628068149 25.9061611658624 27.375206064236636 23.841069963086063 98-99 22.81445463176534 26.024813302161892 27.039335530717917 24.12139653535485 100-101 23.111428054258536 25.33083927968316 27.503279269979352 24.054453396078955 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2343.0 1 1263.5 2 227.5 3 280.5 4 266.0 5 254.0 6 424.0 7 823.0 8 1302.5 9 1586.0 10 1639.0 11 1765.5 12 1978.5 13 2294.0 14 2623.0 15 2867.5 16 3061.5 17 3230.5 18 3376.0 19 3502.5 20 3662.5 21 3936.0 22 4512.5 23 5443.0 24 6713.0 25 8645.0 26 11514.5 27 14902.5 28 18137.0 29 21720.0 30 26617.5 31 32689.0 32 38791.0 33 43707.0 34 48955.5 35 54433.5 36 61257.0 37 69042.0 38 74895.5 39 78917.0 40 82989.5 41 88716.0 42 94353.0 43 99600.5 44 105482.0 45 111520.5 46 114921.0 47 121565.5 48 137149.0 49 138047.0 50 125804.5 51 120699.5 52 118468.5 53 117603.0 54 116364.5 55 109442.0 56 98767.5 57 89146.5 58 80954.0 59 71534.0 60 60418.0 61 50090.0 62 40617.0 63 32001.5 64 24336.0 65 17910.0 66 13124.0 67 10425.5 68 7972.0 69 5704.0 70 4263.0 71 3156.5 72 2411.0 73 1917.5 74 1483.0 75 1060.5 76 721.5 77 440.0 78 268.5 79 191.5 80 134.5 81 89.0 82 73.5 83 60.5 84 41.0 85 30.0 86 22.0 87 18.5 88 15.5 89 17.5 90 14.0 91 10.0 92 10.5 93 7.5 94 4.5 95 4.0 96 4.0 97 2.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3858487960298616 2 0.11401699005520276 3 0.11515164875634921 4 0.12763289446896028 5 0.12912416019046705 6 0.06989497599062189 7 0.07981513492064521 8 0.0768001846575989 9 0.07579520123658347 10-11 0.0740770037748474 12-13 0.07814556568895828 14-15 0.0721642933929148 16-17 0.07383386262460173 18-19 0.08338120512424838 20-21 0.08197098645282348 22-23 0.09143728190238823 24-25 0.10570156271680087 26-27 0.10665791790776716 28-29 0.10064422679169094 30-31 0.09033504202127453 32-33 0.08868168219960397 34-35 0.3374312883109406 36-37 0.5257035856511709 38-39 0.5071438111824181 40-41 0.4792312071342152 42-43 0.5078408158131223 44-45 0.5172260642126053 46-47 0.5147136056600666 48-49 0.5209380191063557 50-51 0.519706103945111 52-53 0.5187335393441284 54-55 0.5132385493485763 56-57 0.4847586159498001 58-59 0.4745953158695311 60-61 0.42363293077803865 62-63 0.4968346264120017 64-65 0.5244392516698935 66-67 0.5356723728112434 68-69 0.5502608418259836 70-71 0.5444416636301038 72-73 0.5313768791569031 74-75 0.5351050434606702 76-77 0.5119255871372497 78-79 0.4861526252112086 80-81 0.4926688080377926 82-83 0.5062685030415337 84-85 0.5011949577064073 86-87 0.5015515647267677 88-89 0.5120066341873315 90-91 0.5294641687749706 92-93 0.536255911571833 94-95 0.530469152195986 96-97 0.5319928367375256 98-99 0.5328033072383445 100-101 0.5098994108852024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3084628.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.854351489455546 #Duplication Level Percentage of deduplicated Percentage of total 1 75.02810337827806 40.40589850920993 2 13.634760257463219 14.685823427597672 3 4.678023749845121 7.5579580590054 4 2.2204530341871984 4.783242326757818 5 1.1851955104875598 3.1913967802760874 6 0.7336609513857931 2.3706500850011314 7 0.4694526976699798 1.7697449411594544 8 0.333421772658925 1.4364970671208868 9 0.24192609088739314 1.1725895459807674 >10 1.3380276861851033 13.132555705163679 >50 0.0926099167562461 3.4319847294457264 >100 0.04122749934308703 3.8942851293133733 >500 0.001991077118253471 0.7320759398400515 >1k 0.0010257063936598145 1.039365871169389 >5k 1.2067134043056641E-4 0.39593188295867093 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6996 0.2268020649491608 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5189 0.16822125714997077 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4301 0.13943334496088344 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3507 0.11369280185487521 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.2418820032755974E-5 0.0 10-11 0.0 0.0 0.0 6.483764006551195E-5 0.0 12-13 0.0 0.0 0.0 9.725646009826792E-5 0.0 14-15 0.0 0.0 0.0 9.725646009826792E-5 0.0 16-17 0.0 0.0 0.0 1.296752801310239E-4 0.0 18-19 0.0 0.0 0.0 2.1072233021291384E-4 0.0 20-21 0.0 0.0 0.0 3.0797879031118177E-4 0.0 22-23 0.0 0.0 0.0 4.214446604258277E-4 0.0 24-25 3.2418820032755974E-5 0.0 0.0 7.132140407206315E-4 0.0 26-27 3.2418820032755974E-5 0.0 0.0 0.0010049834210154353 0.0 28-29 6.483764006551195E-5 0.0 0.0 0.0023503644523748084 0.0 30-31 6.483764006551195E-5 0.0 0.0 0.006921418076993402 0.0 32-33 6.483764006551195E-5 0.0 0.0 0.016873995827049487 0.0 34-35 8.104705008188994E-5 0.0 0.0 0.03296993997331282 0.0 36-37 9.725646009826792E-5 0.0 0.0 0.0636867719543491 0.0 38-39 9.725646009826792E-5 0.0 0.0 0.11088857392204182 0.0 40-41 9.725646009826792E-5 0.0 0.0 0.16301803653471342 0.0 42-43 9.725646009826792E-5 0.0 0.0 0.218275915280546 0.0 44-45 9.725646009826792E-5 0.0 0.0 0.28488038103784313 0.0 46-47 9.725646009826792E-5 0.0 0.0 0.36925036017309054 0.0 48-49 9.725646009826792E-5 0.0 0.0 0.46383226761865615 0.0 50-51 9.725646009826792E-5 0.0 0.0 0.5631797416090368 0.0 52-53 9.725646009826792E-5 0.0 0.0 0.663791549580695 0.0 54-55 9.725646009826792E-5 0.0 0.0 0.7777112831757995 0.0 56-57 9.725646009826792E-5 0.0 0.0 0.9171770469567156 0.0 58-59 9.725646009826792E-5 0.0 0.0 1.0749756534661554 0.0 60-61 9.725646009826792E-5 0.0 0.0 1.2476544983706301 0.0 62-63 9.725646009826792E-5 0.0 0.0 1.4232348276680367 0.0 64-65 9.725646009826792E-5 0.0 0.0 1.6095619958063014 0.0 66-67 1.296752801310239E-4 0.0 0.0 1.8333166916723833 0.0 68-69 1.9451292019653584E-4 0.0 0.0 2.0925537860643164 0.0 70-71 1.9451292019653584E-4 0.0 0.0 2.371841920646509 0.0 72-73 2.1072233021291384E-4 0.0 0.0 2.656236019383861 0.0 74-75 2.2693174022929183E-4 0.0 0.0 2.9494480371701224 0.0 76-77 2.2693174022929183E-4 0.0 0.0 3.2709292660249467 0.0 78-79 2.2693174022929183E-4 0.0 0.0 3.646079851443999 0.0 80-81 2.593505602620478E-4 0.0 0.0 4.064120535766388 0.0 82-83 2.593505602620478E-4 0.0 0.0 4.486067687902723 0.0 84-85 2.755599702784258E-4 0.0 0.0 4.9133801547544795 0.0 86-87 2.917693802948038E-4 0.0 0.0 5.3727872534386645 0.0 88-89 2.917693802948038E-4 0.0 0.0 5.879995902261148 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11635 0.0 24.937893 1 TACATGG 11650 0.0 24.108511 2 ACATGGG 11810 0.0 22.699076 3 GTATCAA 17110 0.0 20.066572 1 CATGGGG 6755 0.0 19.98302 4 GAGTACT 6565 0.0 19.579138 12-13 AGTACAT 8790 0.0 19.236275 2 GTATAGG 820 0.0 18.531946 1 GAGTACA 10255 0.0 18.198761 1 ATCAACG 18730 0.0 18.055191 3 TCAACGC 18775 0.0 18.037144 4 CAACGCA 18735 0.0 18.0248 5 GTACTTT 7040 0.0 17.955917 14-15 AGAGTAC 13160 0.0 17.770857 10-11 AGTACTT 7055 0.0 17.749538 12-13 TACCTGG 2435 0.0 17.700441 2 AACGCAG 19145 0.0 17.679947 6 TATCAAC 19405 0.0 17.549185 2 GTGTAGC 1790 0.0 16.713694 1 GGACCGA 520 0.0 16.386997 6 >>END_MODULE