FastQCFastQC Report
Fri 27 May 2016
SRR939168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3084628
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72720.23574965927820146No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54020.1751264658169478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46950.1522063600537893No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33620.1089920729501256No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG113100.024.9431321
TACATGG112450.024.7291972
ACATGGG115300.023.0890163
GCACCGT7450.021.656816
GGACCGA7150.020.5744156
CATGGGG61300.019.5079614
GAGTACT73350.018.73015612-13
GTATAGG7950.018.5817431
GTACTTT73350.018.34196714-15
TACCTGG24700.018.2514972
TGCACCG7500.017.716125
CATGGGA58150.017.2188684
AGTACTT77600.017.12337312-13
AGTACAT86400.017.026282
GAGTACA99200.016.8131181
GTACCTG27850.016.7684441
GTATCAA182300.016.7295861
ATTCGCG3350.016.30547180-81
AGAGTAC141050.016.30084410-11
GTGTAGC17900.016.2393531
Produced by FastQC (version 0.11.5)