##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939167_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1515674 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.207689780256175 33.0 31.0 34.0 31.0 34.0 2 32.25894552522508 34.0 31.0 34.0 30.0 34.0 3 32.306320488442765 34.0 31.0 34.0 30.0 34.0 4 35.74886090280628 37.0 35.0 37.0 35.0 37.0 5 35.82683941269693 37.0 35.0 37.0 35.0 37.0 6 35.94960525812279 37.0 37.0 37.0 35.0 37.0 7 35.89495828258583 37.0 36.0 37.0 35.0 37.0 8 35.899808929888614 37.0 37.0 37.0 35.0 37.0 9 37.5670638936869 39.0 38.0 39.0 35.0 39.0 10-11 37.48763322455884 39.0 38.0 39.0 35.0 39.0 12-13 37.40605895463008 39.0 37.5 39.0 35.0 39.0 14-15 38.62756206149871 40.0 38.0 41.0 34.5 41.0 16-17 38.609437781475435 40.0 38.0 41.0 34.5 41.0 18-19 38.63889959186474 40.0 38.0 41.0 34.0 41.0 20-21 38.573562322768616 40.0 38.0 41.0 34.0 41.0 22-23 38.48744090087974 40.0 38.0 41.0 34.0 41.0 24-25 38.4970824860755 40.0 38.0 41.0 34.0 41.0 26-27 38.382952732579696 40.0 38.0 41.0 34.0 41.0 28-29 38.26804213834901 40.0 38.0 41.0 34.0 41.0 30-31 38.064412268073475 40.0 38.0 41.0 34.0 41.0 32-33 37.78234534603087 40.0 37.0 41.0 33.5 41.0 34-35 37.4216718106928 40.0 36.5 41.0 33.0 41.0 36-37 37.12583477713545 40.0 36.0 41.0 32.0 41.0 38-39 36.868912774118975 40.0 35.5 41.0 31.0 41.0 40-41 36.72233837883344 40.0 35.0 41.0 31.0 41.0 42-43 36.461199439985116 40.0 35.0 41.0 30.5 41.0 44-45 36.07966257915621 39.0 35.0 41.0 29.5 41.0 46-47 35.51064114050911 39.0 35.0 41.0 26.5 41.0 48-49 35.2646677319793 39.0 35.0 41.0 24.0 41.0 50-51 34.194539524990205 38.0 34.0 40.0 23.0 40.5 52-53 34.365642281915505 38.5 34.5 39.5 23.0 40.5 54-55 34.88113604904484 39.0 35.0 41.0 23.0 41.0 56-57 34.751497023766326 39.0 35.0 41.0 22.5 41.0 58-59 34.58250850776618 39.0 35.0 41.0 22.0 41.0 60-61 34.24910567839786 38.5 35.0 40.5 20.5 41.0 62-63 33.920369419809276 38.0 34.0 40.0 19.5 41.0 64-65 33.5576888565747 37.5 34.0 40.0 17.5 41.0 66-67 33.137229377821356 37.0 34.0 39.5 12.5 41.0 68-69 32.72564087000239 36.0 33.0 39.0 8.0 41.0 70-71 32.28102909992518 35.5 33.0 39.0 7.0 41.0 72-73 31.826278606085477 35.0 32.5 38.0 2.0 40.0 74-75 31.39777650075148 35.0 32.0 37.0 2.0 39.5 76-77 30.976202996158804 35.0 31.5 37.0 2.0 39.0 78-79 30.549360218622212 35.0 31.0 36.0 2.0 39.0 80-81 30.10022141964565 35.0 31.0 36.0 2.0 37.5 82-83 29.68196030280918 35.0 30.5 35.5 2.0 37.0 84-85 29.303444210298522 34.0 30.0 35.0 2.0 37.0 86-87 28.98249293713556 34.0 30.0 35.0 2.0 36.0 88-89 28.56102796511651 34.0 29.0 35.0 2.0 36.0 90-91 28.163305235822477 34.0 29.0 35.0 2.0 36.0 92-93 27.764085152875882 34.0 28.5 35.0 2.0 35.0 94-95 27.314017064355525 34.0 27.0 35.0 2.0 35.0 96-97 26.76657942275186 34.0 25.5 35.0 2.0 35.0 98-99 26.151821895737474 34.0 24.5 35.0 2.0 35.0 100-101 23.7314359816161 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26237239868785167 1101 2 0.3178603565091578 1101 3 0.3368171630873036 1101 4 0.07523718626971032 1101 5 0.2297725601593399 1101 6 0.15082875464415224 1101 7 0.17201261692066794 1101 8 0.22824839750143866 1101 9 0.22963383867907794 1101 10-11 0.257999965148592 1101 12-13 0.2539800126094107 1101 14-15 0.2929662274684972 1101 16-17 0.40034558695401046 1101 18-19 0.4728142810376781 1101 20-21 0.4405412687116055 1101 22-23 0.4657262610059334 1101 24-25 0.4271489751981363 1101 26-27 0.3278182067115054 1101 28-29 0.3434845418356858 1101 30-31 0.3206707495161467 1101 32-33 0.32781065969900425 1101 34-35 0.3904370610981047 1101 36-37 0.3483585855823961 1101 38-39 0.5283518368459639 1101 40-41 0.19734943734875543 1101 42-43 0.33099029998565044 1101 44-45 0.28656143748310825 1101 46-47 0.3325939907150328 1101 48-49 0.45527783828379853 1101 50-51 0.3319276157053892 1101 52-53 0.43480687761726244 1101 54-55 0.3933669159911375 1101 56-57 0.47382846650936017 1101 58-59 0.4797532834078666 1101 60-61 0.31903448719021554 1101 62-63 0.31366042169042885 1101 64-65 0.23707815928440823 1101 66-67 0.25231716366432266 1101 68-69 0.18516673657190807 1101 70-71 0.2738712103822607 1101 72-73 0.22735403091145656 1101 74-75 0.20198326496982943 1101 76-77 0.1280664892880523 1101 78-79 0.3299857073664576 1101 80-81 0.265325232473689 1101 82-83 0.03618935254558764 1101 84-85 0.09344684224213395 1101 86-87 0.08204719613618394 1101 88-89 0.08812034346804865 1101 90-91 0.026377897984101395 1101 92-93 -0.027765191171535974 1101 94-95 -0.17365657162059023 1101 96-97 -0.1013378965131082 1101 98-99 -0.08371600885145014 1101 100-101 -0.39514681377302807 1102 1 0.12809449036664233 1102 2 0.23044054747555265 1102 3 0.18080610419356447 1102 4 0.24633766741551 1102 5 0.19265859574175437 1102 6 0.22373026894798187 1102 7 0.17310824931035995 1102 8 0.2310597435907269 1102 9 0.21678696612676873 1102 10-11 0.1945768365819518 1102 12-13 0.22542713319423768 1102 14-15 0.26489123026585304 1102 16-17 0.3664054597674067 1102 18-19 0.3334335231398029 1102 20-21 0.2829077414280263 1102 22-23 0.24872810726306227 1102 24-25 0.17092018850610913 1102 26-27 0.13000644101832393 1102 28-29 0.2828599381282473 1102 30-31 0.26441701970374965 1102 32-33 0.3456624742527339 1102 34-35 0.23906728749771844 1102 36-37 0.16539147320314385 1102 38-39 0.30050925357790703 1102 40-41 0.2375381658557103 1102 42-43 0.43785874950264514 1102 44-45 0.323460937688246 1102 46-47 0.2582168693842348 1102 48-49 0.3540204252816679 1102 50-51 0.325171993013214 1102 52-53 0.2344227069410394 1102 54-55 0.2128218505329329 1102 56-57 0.1659044800858709 1102 58-59 0.10107242480504652 1102 60-61 0.2435768858325602 1102 62-63 0.17110349542882375 1102 64-65 0.2332562671505798 1102 66-67 0.2871268033644512 1102 68-69 0.09390288964340954 1102 70-71 0.06523016752672106 1102 72-73 0.001135081599610288 1102 74-75 -0.024994468050199004 1102 76-77 0.053385155134158424 1102 78-79 0.09239549252926693 1102 80-81 0.1232601752210698 1102 82-83 -0.03711428215658685 1102 84-85 -0.03670262998941354 1102 86-87 -0.3569610467492019 1102 88-89 -0.37111164153810705 1102 90-91 -0.35007206658267975 1102 92-93 -0.3465837264742113 1102 94-95 -0.45942780357762203 1102 96-97 -0.6690980975499983 1102 98-99 -0.7715726721612839 1102 100-101 -0.5081950961944202 1103 1 0.07743477490714668 1103 2 0.033209857684497024 1103 3 0.20174465304602762 1103 4 0.16006282900389834 1103 5 0.11665203708754746 1103 6 0.12255238357042941 1103 7 0.029988721460988188 1103 8 -0.018982153889531617 1103 9 0.10202750295805174 1103 10-11 0.018255254170739477 1103 12-13 0.08479088032370186 1103 14-15 0.1878712055350178 1103 16-17 0.14663505795925147 1103 18-19 0.26218232849542744 1103 20-21 0.23902975594946696 1103 22-23 0.2001819498281847 1103 24-25 0.06598002263738323 1103 26-27 0.09853180824407559 1103 28-29 0.20008806877591923 1103 30-31 0.15256093868081422 1103 32-33 0.10298094492676313 1103 34-35 -0.05756347567054121 1103 36-37 -0.11778623552557121 1103 38-39 -0.0706044720366279 1103 40-41 -0.13013034128044865 1103 42-43 -0.06698663747155109 1103 44-45 -0.14694981614933766 1103 46-47 0.006585698564812503 1103 48-49 -0.10775661671092962 1103 50-51 0.02897492538388491 1103 52-53 0.20031532709622724 1103 54-55 0.3182013737622569 1103 56-57 0.28646844281749395 1103 58-59 0.10951363471369291 1103 60-61 0.18339455252792192 1103 62-63 0.048284429131157935 1103 64-65 0.11340909104075081 1103 66-67 0.09351288524797496 1103 68-69 -0.20307664173893158 1103 70-71 -0.3984662320076531 1103 72-73 -0.4477511248439967 1103 74-75 -0.33546328953069704 1103 76-77 -0.24806778153949693 1103 78-79 -0.15667200544380222 1103 80-81 -0.19386241907090707 1103 82-83 -0.3334308142228828 1103 84-85 -0.19631221341522576 1103 86-87 -0.29092835312622256 1103 88-89 -0.16773957379554005 1103 90-91 -0.24051411897038477 1103 92-93 -0.34923119428109217 1103 94-95 -0.42597832740936425 1103 96-97 -0.358275978398094 1103 98-99 -0.3503535234274935 1103 100-101 -0.6937301609883058 1104 1 0.14817262804153586 1104 2 0.1799536120772558 1104 3 0.224806779457424 1104 4 0.10157030458395866 1104 5 0.21506727068990017 1104 6 0.28349440892930744 1104 7 0.13611562897965257 1104 8 0.2249626371402087 1104 9 0.2837816911759887 1104 10-11 0.3143517632456607 1104 12-13 0.18537828618534746 1104 14-15 0.2529743578775694 1104 16-17 0.3980178933920371 1104 18-19 0.38766784408306876 1104 20-21 0.4677557412877107 1104 22-23 0.36780858643731307 1104 24-25 0.5158062706164372 1104 26-27 0.5348463070179008 1104 28-29 0.49951056362428403 1104 30-31 0.4265603685740018 1104 32-33 0.4110611210941215 1104 34-35 0.4974784881080865 1104 36-37 0.4657550852509047 1104 38-39 0.42920676167887706 1104 40-41 0.40347658529979924 1104 42-43 0.3057076394828684 1104 44-45 0.4385183351161075 1104 46-47 0.47654030761732713 1104 48-49 0.5117438652686914 1104 50-51 0.4083467416528137 1104 52-53 0.43246630530813235 1104 54-55 0.420232542502994 1104 56-57 0.38309937263560556 1104 58-59 0.3698729325683914 1104 60-61 0.35349745463273763 1104 62-63 0.442700428678549 1104 64-65 0.533161116334341 1104 66-67 0.5641099524618269 1104 68-69 0.40063601572043694 1104 70-71 0.5412944754470317 1104 72-73 0.5369865498600177 1104 74-75 0.4369117711247519 1104 76-77 0.386515507367573 1104 78-79 0.32934568962738453 1104 80-81 0.31758214892085945 1104 82-83 0.44536338809540865 1104 84-85 0.45222074071728713 1104 86-87 0.39730100824534276 1104 88-89 0.4696801053344153 1104 90-91 0.44884784010813306 1104 92-93 0.2118550985666765 1104 94-95 0.003603144554222837 1104 96-97 0.04362920108763291 1104 98-99 -0.029631990144789455 1104 100-101 0.05311085972049767 1105 1 0.09287780824310232 1105 2 0.21303691036951022 1105 3 0.31814942693514325 1105 4 0.15819442460077937 1105 5 0.16704290922875487 1105 6 0.26815319926610215 1105 7 0.14638379495227838 1105 8 0.22428442274306803 1105 9 0.3208868008432546 1105 10-11 0.15623964084153386 1105 12-13 0.3337817694945002 1105 14-15 0.3091474653202937 1105 16-17 0.30482106200970094 1105 18-19 0.2911409800107947 1105 20-21 0.419124377941138 1105 22-23 0.28503591033894793 1105 24-25 0.2875023491807909 1105 26-27 0.390780004778442 1105 28-29 0.47074749104177727 1105 30-31 0.374483676089973 1105 32-33 0.3811484751084748 1105 34-35 0.3754609359194845 1105 36-37 0.09325939191233346 1105 38-39 0.2848081343848179 1105 40-41 0.2987908020299983 1105 42-43 0.24382057574427307 1105 44-45 0.13019189696247935 1105 46-47 0.20589546136853443 1105 48-49 0.21806007594921084 1105 50-51 0.3688814458835239 1105 52-53 0.2671006799266351 1105 54-55 0.24298799195567256 1105 56-57 0.1667862817413095 1105 58-59 0.18697885609294218 1105 60-61 0.21417526210123583 1105 62-63 0.26852818935025624 1105 64-65 0.24575081018836187 1105 66-67 0.1958022330255531 1105 68-69 0.229709032282436 1105 70-71 0.06934542291254786 1105 72-73 0.12366913195786822 1105 74-75 0.2083569103621663 1105 76-77 0.23111624255197682 1105 78-79 0.10658277981633546 1105 80-81 -0.02809016501102235 1105 82-83 -0.0043809250768731545 1105 84-85 0.1264472371973575 1105 86-87 0.14126829118282203 1105 88-89 0.09779480495325643 1105 90-91 0.030989776817165193 1105 92-93 0.009374190301958407 1105 94-95 0.005356328770812269 1105 96-97 0.12160351032627403 1105 98-99 0.2428575992642159 1105 100-101 0.42762424089055173 1106 1 0.08647262886152873 1106 2 0.16901907172154296 1106 3 0.09138140246792403 1106 4 0.07343241539714285 1106 5 0.012140733417346894 1106 6 0.05767380591010607 1106 7 0.06472029635150989 1106 8 0.09806948978015839 1106 9 0.0766598901542821 1106 10-11 0.08521277117495174 1106 12-13 0.12246060478349818 1106 14-15 0.16739733230213716 1106 16-17 0.14352538398267 1106 18-19 0.09335466605546117 1106 20-21 0.25917487406837836 1106 22-23 0.1559334173147775 1106 24-25 0.22331449099642242 1106 26-27 0.20134692610886873 1106 28-29 0.18826770776997392 1106 30-31 0.14210959655527233 1106 32-33 -0.04627387681490802 1106 34-35 0.051009356557408125 1106 36-37 0.12479586325554948 1106 38-39 0.07804842609058227 1106 40-41 0.12579942688631718 1106 42-43 -0.045239036695662094 1106 44-45 -0.10602853949952618 1106 46-47 -0.19532764561055416 1106 48-49 -0.15190985788694888 1106 50-51 -0.05566654625317824 1106 52-53 -0.13266278130719655 1106 54-55 -0.2145073777941704 1106 56-57 -0.1917527220238071 1106 58-59 -0.16877412818451631 1106 60-61 -0.1668085238999737 1106 62-63 -0.014866728340955149 1106 64-65 -0.06092482251095532 1106 66-67 -0.06173258523712377 1106 68-69 -0.07966377190266627 1106 70-71 -0.24511811792910265 1106 72-73 -0.12322271108477167 1106 74-75 -0.20883751343569656 1106 76-77 -0.04963151238593966 1106 78-79 -0.24881608171075342 1106 80-81 -0.02990520276381048 1106 82-83 0.12359649839199705 1106 84-85 0.17491001445389287 1106 86-87 0.08513361588927282 1106 88-89 0.23887756255154002 1106 90-91 0.3485033426377946 1106 92-93 0.31465084774874086 1106 94-95 0.37801480314400493 1106 96-97 0.4205612978790221 1106 98-99 0.469485497739889 1106 100-101 0.6485600286626294 1107 1 -0.011717297483471611 1107 2 0.1329992242871043 1107 3 0.048837522479985296 1107 4 0.07790349827254772 1107 5 0.1622756001350254 1107 6 0.1975989915933667 1107 7 0.22255010150967536 1107 8 0.096674450093019 1107 9 0.34015912114348623 1107 10-11 0.18531604661993129 1107 12-13 0.32173294280818965 1107 14-15 0.3783816274786247 1107 16-17 0.3551898093113408 1107 18-19 0.28748448589826125 1107 20-21 0.19695793960045904 1107 22-23 0.3649415672834877 1107 24-25 0.2783316101815032 1107 26-27 0.2864887873468547 1107 28-29 0.17672868143812082 1107 30-31 0.2953465909982995 1107 32-33 0.16784249499988135 1107 34-35 0.11052074487307806 1107 36-37 0.17703642838187506 1107 38-39 0.27998476822696006 1107 40-41 0.08153058612178654 1107 42-43 0.03218180156474659 1107 44-45 0.21881959052900157 1107 46-47 0.3352835247543169 1107 48-49 0.2063829961091983 1107 50-51 0.21699753514603515 1107 52-53 0.19085938475276265 1107 54-55 0.22434901813432617 1107 56-57 0.15125749152159784 1107 58-59 0.07138427258217206 1107 60-61 -0.05218823747242851 1107 62-63 0.12493334817368407 1107 64-65 -0.010991772822805501 1107 66-67 -0.004239367216761991 1107 68-69 0.26308511322489636 1107 70-71 0.16428642039391406 1107 72-73 0.2226563184931578 1107 74-75 0.23698731827073516 1107 76-77 0.16364001025544184 1107 78-79 0.2827283354254355 1107 80-81 0.2538530186958461 1107 82-83 0.19876102419443598 1107 84-85 0.16761697110332463 1107 86-87 7.742907638714769E-4 1107 88-89 0.19565789983426285 1107 90-91 0.14122353477456429 1107 92-93 0.18382819941467332 1107 94-95 0.2714053678838937 1107 96-97 0.3272675357545438 1107 98-99 0.279390086299788 1107 100-101 0.3768581768537125 1108 1 0.06436454234665945 1108 2 0.13939514887728421 1108 3 0.21407586117933164 1108 4 0.09653348256600935 1108 5 0.19244091032769006 1108 6 0.2515161844982501 1108 7 0.18332013656151958 1108 8 0.24112718538022904 1108 9 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Count 2 4285.0 3 934.0 4 943.0 5 1044.0 6 1236.0 7 1484.0 8 1812.0 9 2298.0 10 2903.0 11 3894.0 12 5890.0 13 10274.0 14 9381.0 15 10835.0 16 14902.0 17 22608.0 18 21117.0 19 10411.0 20 9258.0 21 9166.0 22 9632.0 23 10273.0 24 11188.0 25 12696.0 26 14414.0 27 16535.0 28 19535.0 29 23237.0 30 27795.0 31 34473.0 32 43655.0 33 57122.0 34 81032.0 35 124257.0 36 188788.0 37 304651.0 38 324728.0 39 66923.0 40 65.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.76453356547759 19.15468155744081 14.301299853365155 19.779485023716447 2 15.050267306202178 22.347762957893575 39.487992855099755 23.113976880804486 3 21.474063427226202 27.455803593839583 29.90262390978675 21.16750906914746 4 11.817619115879982 16.796021136227466 39.14284525224287 32.24351449564969 5 12.098952386993107 40.2345897289526 32.87024557063724 14.796212313417056 6 28.304391238654738 37.01211394397895 18.201227243430576 16.482267573935736 7 26.715771579601537 35.66957012177605 20.644140398752324 16.970517899870092 8 26.414715511428216 31.72657342334042 20.124852800635097 21.733858264596265 9 27.413119508883522 17.787717333305157 21.97212639275582 32.8270367650555 10-11 25.065492782421323 26.912333114748904 26.257999628868728 21.76417447396105 12-13 28.029980587704845 25.261309832976337 26.856162454395633 19.852547124923188 14-15 23.80911970006713 25.781011890394005 28.76614171590316 21.643726693635706 16-17 20.26045171289164 29.533852053231413 28.71572252205374 21.489973711823207 18-19 19.893656226017832 28.48655281591075 32.01375560877221 19.60603534929921 20-21 22.180144840570684 25.80492394532531 31.20393233992257 20.810998874181436 22-23 20.944014960081457 26.400336086605712 31.43635079171581 21.21929816159702 24-25 21.596533508798355 26.38912011162112 31.770366758891505 20.243979620689018 26-27 21.188439642401192 25.563062877074998 33.341018657831846 19.907478822691964 28-29 19.7217965965433 27.777731903054892 32.51608799006728 19.98438351033453 30-31 20.25653914570179 26.72566798399652 32.08393965074796 20.93385321955373 32-33 20.272006309077423 26.932534747151436 32.124313659958425 20.671145283812713 34-35 21.54426997054615 26.36627426864332 31.913520405039225 20.1759353557713 36-37 21.182714107035533 25.896982251796487 32.794877124379305 20.125426516788675 38-39 20.668016180914446 26.920986471053382 32.45588394177469 19.955113406257478 40-41 20.39535920132935 27.40738531783308 31.993744232550203 20.203511248287366 42-43 19.822879319756886 27.818632772065595 30.85677712485652 21.501710783321 44-45 20.585037549323264 26.96590330518902 30.773109009716155 21.675950135771565 46-47 21.57427463085949 27.83946970796943 30.140966557736153 20.445289103434924 48-49 21.34203369522586 27.57877022404802 30.091506969030984 20.98768911169513 50-51 20.15232872134969 28.15555350765124 31.03657918063643 20.655538590362635 52-53 20.939356760177567 29.0379514611417 29.16067602604466 20.862015752636076 54-55 19.859107871012736 27.836719460058244 30.06700878180978 22.237163887119237 56-57 21.252754681716205 27.86829887426721 29.152336798571042 21.72660964544554 58-59 22.270412042846015 27.28688401564266 29.191357507246973 21.251346434264352 60-61 21.12982476127897 27.78632613372617 30.767821942198246 20.316027162796612 62-63 20.84117706325449 27.796333906919894 30.316524330587363 21.04596469923825 64-65 20.20298028877266 28.934023615280974 29.85745695462598 21.005539141320376 66-67 20.691518743165812 28.027549612312114 28.97470430219828 22.306227342323794 68-69 20.276222941426145 28.127089081069613 29.61882560483871 21.977862372665534 70-71 21.24442251637228 28.18465735674111 29.02867813845257 21.542241988434046 72-73 21.07979038372404 27.353823282312913 30.126968633814048 21.439417700148997 74-75 19.920622289420283 29.49021207177814 29.214445814931235 21.374719823870343 76-77 20.48507048323071 27.848674451175548 29.26884732362618 22.397407741967566 78-79 20.637704560534143 28.020211299954067 29.648078660481048 21.694005479030743 80-81 21.110981854723114 28.264527506587932 29.08281987061767 21.541670768071285 82-83 20.73767424315499 27.90197534964647 29.700550212756227 21.659800194442315 84-85 20.301161109061518 28.223677820990524 29.40885166250837 22.066309407439597 86-87 20.4084832453253 28.43465030479619 29.158238778561174 21.998627671317337 88-89 20.4406192698493 28.111010807285925 28.612669909172915 22.83570001369186 90-91 20.573905350910408 27.394129377054526 29.20307649661892 22.828888775416146 92-93 21.193791530758872 28.015395847272238 29.313579153208508 21.47723346876038 94-95 20.753629070093996 27.615415553020185 29.375300274238036 22.255655102647783 96-97 19.79248694381272 27.50643815672597 30.170924896294864 22.530150003166444 98-99 20.492252767910603 27.961351036113964 28.66528920413743 22.881106991838003 100-101 20.532788787302174 26.711938685531294 29.74484349826625 23.01042902890028 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3726.0 1 2302.5 2 1208.5 3 1572.5 4 1303.5 5 1173.0 6 2176.5 7 4292.0 8 6474.0 9 7055.0 10 5818.5 11 4691.0 12 4597.0 13 4775.0 14 4860.5 15 5009.5 16 5209.0 17 5238.5 18 5236.5 19 5363.0 20 5695.5 21 6173.0 22 6810.0 23 7932.5 24 9080.0 25 10220.0 26 12255.5 27 14509.0 28 15736.0 29 17235.5 30 19810.0 31 22503.5 32 25601.5 33 29052.0 34 32818.0 35 35039.0 36 37567.0 37 41086.0 38 41602.0 39 40601.0 40 40085.0 41 41270.5 42 42360.5 43 43354.5 44 45396.0 45 46603.0 46 48222.5 47 61790.5 48 97569.5 49 95440.0 50 59765.5 51 44918.0 52 38216.0 53 36388.5 54 35311.5 55 32172.0 56 28804.0 57 26190.5 58 24688.5 59 22363.5 60 19624.0 61 17215.0 62 14337.0 63 10591.0 64 7327.0 65 5767.0 66 4202.0 67 3212.5 68 2441.0 69 1649.5 70 1316.0 71 1093.0 72 912.0 73 916.0 74 744.0 75 585.5 76 473.5 77 270.0 78 159.0 79 99.5 80 64.0 81 52.0 82 43.0 83 29.0 84 18.5 85 15.0 86 13.5 87 10.0 88 5.0 89 4.0 90 5.0 91 4.0 92 2.5 93 3.0 94 3.0 95 3.5 96 4.0 97 2.5 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3377375345885725 2 0.12317952277336683 3 0.11546018470990464 4 0.12370734076061211 5 0.14105935709130066 6 0.09144446629024447 7 0.09903184985689535 8 0.10365025724529153 9 0.09302792025198031 10-11 0.09137848904183882 12-13 0.09418252209907936 14-15 0.0968875892837114 16-17 0.08610030916938602 18-19 0.10081323556384816 20-21 0.10981912997122072 22-23 0.11734053628946595 24-25 0.1307669063400177 26-27 0.12974425898973 28-29 0.12466401086249418 30-31 0.11565811645512163 32-33 0.10985211859542356 34-35 0.33024911689453007 36-37 0.48694508185797214 38-39 0.4607191256167223 40-41 0.43983732649633095 42-43 0.472034223718293 44-45 0.4788628689282788 46-47 0.4864502524949296 48-49 0.4973035098576607 50-51 0.4853946165204391 52-53 0.48945221729738714 54-55 0.47602584724683544 56-57 0.45385749178253376 58-59 0.4351529418595292 60-61 0.39335635499454363 62-63 0.4643478742790337 64-65 0.4946974085456371 66-67 0.5034723825835898 68-69 0.5168657640099389 70-71 0.5089155055770569 72-73 0.5004374291569296 74-75 0.5076289492331464 76-77 0.484042082928123 78-79 0.4626984430688921 80-81 0.4647767263936704 82-83 0.47882988030407597 84-85 0.47803815332320804 86-87 0.48090816362885425 88-89 0.49341085220172676 90-91 0.5096742439337218 92-93 0.5017569741250427 94-95 0.506540324634453 96-97 0.5062104383924247 98-99 0.5005693836537408 100-101 0.4871760022273919 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1515674.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.33136496681368 #Duplication Level Percentage of deduplicated Percentage of total 1 75.20925423164329 34.09338152456498 2 13.33544770932504 12.090280942145458 3 4.750913308448279 6.460961553328836 4 2.123856317990578 3.85109223551616 5 1.1601780456305655 2.6296227206481895 6 0.7126811786583624 1.9384086368844706 7 0.4738314130683325 1.5035597302979142 8 0.3349984517994212 1.2148749665469685 9 0.23859553586721235 0.973427518426418 >10 1.429187486802425 12.249989379971108 >50 0.14205789055158652 4.4320802835412305 >100 0.07863963121757435 6.562142785964713 >500 0.004231058745360766 1.2581396854571594 >1k 0.0049605516324919335 5.194067290001185 >5k 5.835943097049334E-4 1.8485519743745782 >10k+ 5.835943097049334E-4 3.6994187723306635 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18838 1.2428794054658192 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15270 1.007472583154425 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11276 0.7439594530222199 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10551 0.6961259479281164 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7974 0.5261025787867312 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7677 0.5065073360102502 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7083 0.46731685045728827 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5216 0.34413732768392147 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4738 0.3126002029460161 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4346 0.28673712157099746 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4204 0.27736835229739376 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4179 0.27571892108725227 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3871 0.25539792857830906 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3859 0.25460620159744113 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3631 0.2395633889609507 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3470 0.22894105196763948 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2811 0.18546204526830967 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2689 0.17741282096281918 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2634 0.1737840723005079 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2569 0.16949555115414 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2507 0.1654049617529891 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2309 0.15234146656866845 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2243 0.14798696817389492 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2196 0.14488603749882892 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2069 0.13650692695131011 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2026 0.13366990526986675 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1663 0.10972016409861224 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1601 0.10562957469746133 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1593 0.10510175671021606 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1564 0.10318841650645191 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1552 0.102396689525584 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1534 0.10120909905428212 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1525 0.10061530381863118 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.597724840565979E-5 0.0 16-17 0.0 0.0 0.0 1.3195449681131959E-4 0.0 18-19 0.0 0.0 0.0 1.6494312101414946E-4 0.0 20-21 0.0 0.0 0.0 2.6390899362263917E-4 0.0 22-23 0.0 0.0 0.0 0.0010556359744905567 0.0 24-25 0.0 0.0 0.0 0.0024411581910094124 0.0 26-27 0.0 0.0 0.0 0.003496794165499969 0.0 28-29 0.0 0.0 0.0 0.008082212929693324 0.0 30-31 0.0 0.0 0.0 0.018044777438947952 0.0 32-33 0.0 0.0 0.0 0.03912450830455626 0.0 34-35 0.0 0.0 0.0 0.07267393911883427 0.0 36-37 0.0 0.0 0.0 0.12288262515554137 0.0 38-39 0.0 0.0 0.0 0.20241819810856426 0.0 40-41 0.0 0.0 0.0 0.29782129930314827 0.0 42-43 0.0 0.0 0.0 0.3847793127018079 0.0 44-45 0.0 0.0 0.0 0.492784068341873 0.0 46-47 0.0 0.0 0.0 0.6313362899937586 0.0 48-49 0.0 0.0 0.0 0.7845354607917006 0.0 50-51 0.0 0.0 0.0 0.9675563478690008 0.0 52-53 0.0 0.0 0.0 1.1437485897363153 0.0 54-55 0.0 0.0 0.0 1.318159445896677 0.0 56-57 0.0 0.0 0.0 1.5429109425905572 0.0 58-59 0.0 0.0 0.0 1.7953728836148142 0.0 60-61 0.0 0.0 0.0 2.10167225933809 0.0 62-63 0.0 0.0 0.0 2.3947431967560306 0.0 64-65 0.0 0.0 0.0 2.675707309091533 0.0 66-67 0.0 0.0 0.0 3.0081666638076525 0.0 68-69 0.0 0.0 0.0 3.3950902370826443 0.0 70-71 0.0 0.0 0.0 3.8319915760249237 0.0 72-73 0.0 0.0 0.0 4.271301084534009 0.0 74-75 0.0 0.0 0.0 4.717604181374095 0.0 76-77 0.0 0.0 0.0 5.1722863887617 0.0 78-79 0.0 0.0 0.0 5.726759184362864 0.0 80-81 0.0 0.0 0.0 6.35176165850968 0.0 82-83 0.0 0.0 0.0 6.959972922937254 0.0 84-85 0.0 0.0 0.0 7.571977879148155 0.0 86-87 0.0 0.0 0.0 8.186423993550065 0.0 88-89 0.0 0.0 0.0 8.843491410422029 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCACGC 60 0.003998893 31.589378 3 GTACATG 15050 0.0 24.638174 1 TACATGG 14895 0.0 24.39985 2 TACCTGG 1240 0.0 24.074163 2 GAGTACT 10430 0.0 23.817312 12-13 ACATGGG 14960 0.0 23.058556 3 AGTACTT 10510 0.0 22.531954 12-13 CATGGGA 9810 0.0 21.97732 4 GTACTTT 11255 0.0 21.92601 14-15 ATGGGAT 3425 0.0 21.443884 5 ATGGGAG 2670 0.0 20.408869 5 GTAAGCG 140 1.5570549E-9 20.380169 94-95 CATATAG 405 0.0 19.889608 3 CATGGGG 3975 0.0 19.788063 4 ACTTTTT 12665 0.0 19.73133 16-17 ATGGGAC 1525 0.0 19.575058 5 TACTTTT 12365 0.0 19.00006 14-15 AGGACCG 225 1.7078237E-6 18.953627 5 GGGACCG 125 0.006117741 18.953627 5 CGATCGT 175 6.730261E-11 18.953001 24-25 >>END_MODULE