##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939167_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1515674 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45534791782402 34.0 31.0 34.0 31.0 34.0 2 32.62829473884226 34.0 31.0 34.0 31.0 34.0 3 32.71252129415692 34.0 31.0 34.0 31.0 34.0 4 36.175584591409496 37.0 35.0 37.0 35.0 37.0 5 36.16282063293294 37.0 35.0 37.0 35.0 37.0 6 36.241714247258976 37.0 37.0 37.0 35.0 37.0 7 36.19599927161118 37.0 37.0 37.0 35.0 37.0 8 36.201108549727714 37.0 37.0 37.0 35.0 37.0 9 37.903853335215885 39.0 38.0 39.0 35.0 39.0 10-11 37.850109588209605 39.0 38.0 39.0 35.0 39.0 12-13 37.786709411126665 39.0 38.0 39.0 35.0 39.0 14-15 39.02847083211825 40.0 38.0 41.0 36.0 41.0 16-17 39.01342373096062 40.0 38.0 41.0 36.0 41.0 18-19 39.13709148537218 40.5 38.5 41.0 36.0 41.0 20-21 39.222736551527575 41.0 39.0 41.0 36.0 41.0 22-23 39.16858341569493 41.0 39.0 41.0 35.5 41.0 24-25 39.08635926986938 41.0 39.0 41.0 35.0 41.0 26-27 38.91030327102002 40.0 38.0 41.0 35.0 41.0 28-29 38.762086042249194 40.0 38.0 41.0 35.0 41.0 30-31 38.5197311559082 40.0 38.0 41.0 34.5 41.0 32-33 38.270989671921534 40.0 37.0 41.0 34.0 41.0 34-35 38.12770490224151 40.0 37.0 41.0 34.0 41.0 36-37 37.96741218758123 40.0 37.0 41.0 33.5 41.0 38-39 37.79317914010532 40.0 37.0 41.0 33.0 41.0 40-41 37.61307411752131 40.0 37.0 41.0 33.0 41.0 42-43 37.36809267692129 40.0 37.0 41.0 33.0 41.0 44-45 37.009325224289654 40.0 36.0 41.0 32.0 41.0 46-47 36.49436818207609 40.0 36.0 41.0 30.5 41.0 48-49 36.00180084899523 40.0 35.5 41.0 29.5 41.0 50-51 35.68584141444664 39.5 35.0 41.0 27.5 41.0 52-53 35.46559616381887 39.0 35.0 41.0 27.0 41.0 54-55 35.242695988715255 39.0 35.0 41.0 26.0 41.0 56-57 34.96700510795857 39.0 35.0 40.5 25.5 41.0 58-59 34.69385534092423 38.0 35.0 40.0 25.0 41.0 60-61 34.292587983959606 38.0 34.0 40.0 23.0 41.0 62-63 33.94548728816355 37.5 34.0 40.0 22.5 41.0 64-65 33.55031655883785 37.0 34.0 40.0 22.0 41.0 66-67 33.16306375909332 36.5 33.0 39.0 20.5 41.0 68-69 32.697135399828724 36.0 33.0 39.0 18.5 40.0 70-71 32.25661553869763 35.0 32.5 38.5 16.5 40.0 72-73 31.794881683000433 35.0 32.0 37.5 10.5 40.0 74-75 31.327381085906335 35.0 31.5 37.0 7.5 39.0 76-77 29.37852598909792 33.5 28.5 35.0 4.5 37.5 78-79 30.327307851160604 34.0 31.0 36.0 2.0 38.0 80-81 30.33483420577248 35.0 31.0 36.0 2.0 37.0 82-83 30.03963913084212 34.5 31.0 35.0 2.0 37.0 84-85 29.667419247146814 34.0 30.5 35.0 2.0 36.5 86-87 29.17491459245194 34.0 30.0 35.0 2.0 36.0 88-89 28.628852246591286 34.0 29.0 35.0 2.0 36.0 90-91 28.16502955120956 34.0 29.0 35.0 2.0 35.0 92-93 27.91416129062054 34.0 29.0 35.0 2.0 35.0 94-95 27.604662348235834 34.0 27.5 35.0 2.0 35.0 96-97 27.05857196204461 33.5 26.5 35.0 2.0 35.0 98-99 26.37129686199011 33.0 25.0 35.0 2.0 35.0 100-101 22.94301874941445 29.0 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2223577289878449 1101 2 0.26920829477144537 1101 3 0.2674778534104334 1101 4 0.18364923456990567 1101 5 0.22790613612187371 1101 6 0.234955076261528 1101 7 0.19947817907301868 1101 8 0.1777050108613878 1101 9 0.21157361279897202 1101 10-11 0.2532956987665713 1101 12-13 0.2555243224650141 1101 14-15 0.3342414567777112 1101 16-17 0.3941121236296965 1101 18-19 0.3222020974474802 1101 20-21 0.3714177788331128 1101 22-23 0.2636433964659304 1101 24-25 0.24066364405043572 1101 26-27 0.3027971648002463 1101 28-29 0.2674782184252109 1101 30-31 0.26949805379813796 1101 32-33 0.3321932983335074 1101 34-35 0.35636870029551204 1101 36-37 0.36321723085310964 1101 38-39 0.44294588666833334 1101 40-41 0.26953785120782214 1101 42-43 0.2591972258055719 1101 44-45 0.3493291909331546 1101 46-47 0.39892740955105666 1101 48-49 0.43610744928718503 1101 50-51 0.40078048726901727 1101 52-53 0.28283303112711167 1101 54-55 0.2998686455526993 1101 56-57 0.20167075037053905 1101 58-59 0.1908466712269572 1101 60-61 0.2795881683259651 1101 62-63 0.3571042649560212 1101 64-65 0.30116660563160735 1101 66-67 0.2914870328303749 1101 68-69 0.3237273995487868 1101 70-71 0.4712789060886493 1101 72-73 0.37155811819755513 1101 74-75 0.5691527050649157 1101 76-77 0.295102375079356 1101 78-79 0.4564995901470894 1101 80-81 0.36647127194254026 1101 82-83 0.5364337668645973 1101 84-85 0.47105424737707935 1101 86-87 0.5165908063434763 1101 88-89 0.7519644579634566 1101 90-91 0.7618192707819915 1101 92-93 0.7678455989323005 1101 94-95 0.5793498196126841 1101 96-97 0.48391958739557595 1101 98-99 0.4856134597610975 1101 100-101 0.15014777797494716 1102 1 0.1865561923051402 1102 2 0.09438033236795462 1102 3 0.07121745655839362 1102 4 0.13665592958314932 1102 5 0.16047579036826676 1102 6 0.1423811327308826 1102 7 0.15076682925391793 1102 8 0.1015049437247697 1102 9 0.16801406634416338 1102 10-11 0.19103347430646522 1102 12-13 0.13568778149302574 1102 14-15 0.2698136099984083 1102 16-17 0.22748858291879515 1102 18-19 0.13081609916319792 1102 20-21 0.06647764232197773 1102 22-23 0.16229740041953278 1102 24-25 0.16134568686867823 1102 26-27 0.2000594825056652 1102 28-29 0.17465834039007433 1102 30-31 0.10874547090305242 1102 32-33 0.22025580066463846 1102 34-35 0.2522638086465605 1102 36-37 0.4450411557346925 1102 38-39 0.43334161976294894 1102 40-41 0.4241863630126801 1102 42-43 0.28357504647619436 1102 44-45 0.3063943880537394 1102 46-47 0.28758901299740103 1102 48-49 0.34079209364956853 1102 50-51 0.3739538116508001 1102 52-53 0.2748850340363589 1102 54-55 0.1776240444561381 1102 56-57 0.12303464951641274 1102 58-59 0.16185087659042097 1102 60-61 0.08798420913011995 1102 62-63 0.24079589339210372 1102 64-65 0.051122314109470324 1102 66-67 0.06229239257078234 1102 68-69 -0.07837784643234613 1102 70-71 -0.020259082050543498 1102 72-73 -0.13544577013186654 1102 74-75 -0.025771020271701417 1102 76-77 -0.026641731817065306 1102 78-79 0.03543291042607777 1102 80-81 0.015610151506898262 1102 82-83 0.2110216225954531 1102 84-85 0.2460125201009582 1102 86-87 0.172986502139981 1102 88-89 0.023548416040366504 1102 90-91 0.2474198638221594 1102 92-93 0.22278363742396223 1102 94-95 5.283937054052501E-4 1102 96-97 -0.13049823551293116 1102 98-99 -0.282332765668766 1102 100-101 -0.49791644939442037 1103 1 0.0032196128607253627 1103 2 -0.09778529176861639 1103 3 -0.004482231361997435 1103 4 -0.015652734815773783 1103 5 0.08899642431111232 1103 6 0.0015116128080663316 1103 7 0.01836965925885181 1103 8 0.002459300383293339 1103 9 -0.053782135252923524 1103 10-11 0.011420108783781302 1103 12-13 0.0038318816483879914 1103 14-15 0.08412114656245961 1103 16-17 0.14978347776744272 1103 18-19 0.1496541121808619 1103 20-21 0.118870441390726 1103 22-23 0.07757786441714387 1103 24-25 0.10392009452889539 1103 26-27 0.043910404476697806 1103 28-29 0.1090140477327779 1103 30-31 0.12032842564924096 1103 32-33 0.1548855401755418 1103 34-35 0.15925140246294944 1103 36-37 0.27753842673665474 1103 38-39 0.20696896772039253 1103 40-41 0.27571358278648006 1103 42-43 0.2252899896088536 1103 44-45 0.37989955389982555 1103 46-47 0.43202717911071886 1103 48-49 0.33746464106166485 1103 50-51 0.43267426260356956 1103 52-53 0.4439521243582689 1103 54-55 0.43002578920309986 1103 56-57 0.5091686218052018 1103 58-59 0.48671204872339047 1103 60-61 0.34218244988889523 1103 62-63 0.3731755117400297 1103 64-65 0.4213414719021742 1103 66-67 0.36973610273986424 1103 68-69 0.4127529099417373 1103 70-71 0.26118950634408833 1103 72-73 0.05169895055914964 1103 74-75 0.12133726093512465 1103 76-77 0.30036074126663337 1103 78-79 0.2739201622684355 1103 80-81 0.2707193793703091 1103 82-83 0.30388568905164703 1103 84-85 0.22487467512616632 1103 86-87 0.0839922907864441 1103 88-89 0.1298808238545881 1103 90-91 0.14968436601215274 1103 92-93 0.2695548137693251 1103 94-95 0.032641113780414344 1103 96-97 -0.11858066710680149 1103 98-99 0.0038745838286935452 1103 100-101 -0.17680680029147666 1104 1 0.03816476727916296 1104 2 0.07211306819508678 1104 3 0.21046979915394814 1104 4 0.0603346706106862 1104 5 0.07766827126136633 1104 6 0.09291257415613074 1104 7 0.15618319080947884 1104 8 0.055144133583532096 1104 9 0.17546608533924513 1104 10-11 0.1748775672824081 1104 12-13 0.09306284671738041 1104 14-15 0.0823286828523635 1104 16-17 0.14123568344979986 1104 18-19 0.18488105603838534 1104 20-21 0.12904423693126432 1104 22-23 0.16484772730863284 1104 24-25 0.10650461504077668 1104 26-27 0.18515708882683413 1104 28-29 0.25217994664109256 1104 30-31 0.23293921024244213 1104 32-33 0.27751181774793565 1104 34-35 0.3636395929830769 1104 36-37 0.4276081669028571 1104 38-39 0.42216742799678997 1104 40-41 0.5177723429564693 1104 42-43 0.4744587651630212 1104 44-45 0.4478325685245892 1104 46-47 0.5463515481308505 1104 48-49 0.4995224002807461 1104 50-51 0.574388238706959 1104 52-53 0.5894653619339678 1104 54-55 0.6406873307110104 1104 56-57 0.6349756384710332 1104 58-59 0.5063138225824702 1104 60-61 0.656374954507406 1104 62-63 0.5024140492202065 1104 64-65 0.5730459172121769 1104 66-67 0.6254284670076586 1104 68-69 0.542891759239879 1104 70-71 0.47940931516246366 1104 72-73 0.4794422632279236 1104 74-75 0.45079589881502713 1104 76-77 0.4224838234120618 1104 78-79 0.5299661233227404 1104 80-81 0.5257007420996835 1104 82-83 0.61897197754746 1104 84-85 0.5207813299264856 1104 86-87 0.633597023978627 1104 88-89 0.840947641679346 1104 90-91 0.858561059740758 1104 92-93 0.9206216968734928 1104 94-95 0.8485161951076741 1104 96-97 0.8578540110548438 1104 98-99 0.7697886152019784 1104 100-101 0.3502297540003205 1105 1 0.06935584740341483 1105 2 0.08198786981737527 1105 3 0.09387117999845884 1105 4 0.07029764010460582 1105 5 0.09184295216149252 1105 6 0.08396014411394503 1105 7 0.11536824838987769 1105 8 0.08141137718457969 1105 9 0.11275160003614815 1105 10-11 0.15253718504077085 1105 12-13 0.031121525827018104 1105 14-15 0.0907826253126558 1105 16-17 0.22906105680682032 1105 18-19 0.15401551882477094 1105 20-21 0.21577768510890394 1105 22-23 0.2197695291886319 1105 24-25 0.10961422805164034 1105 26-27 0.16574659278082748 1105 28-29 0.2935544159383241 1105 30-31 0.11279013871794774 1105 32-33 0.129050686215038 1105 34-35 0.1700738129686954 1105 36-37 0.20434676950991104 1105 38-39 0.2607150996867631 1105 40-41 0.19645809909770406 1105 42-43 0.32581790681488343 1105 44-45 0.3714011970394324 1105 46-47 0.10984597999065926 1105 48-49 0.14676613697078977 1105 50-51 0.14008008521347648 1105 52-53 0.12652977873740667 1105 54-55 0.12001535588633061 1105 56-57 0.12672855140186812 1105 58-59 0.14660586170187884 1105 60-61 0.1936801374218291 1105 62-63 0.20224992133610442 1105 64-65 0.22804164446147013 1105 66-67 0.16636887909072584 1105 68-69 0.11193737594022934 1105 70-71 0.1570099282682733 1105 72-73 -4.6224418495910413E-4 1105 74-75 0.05641761795287792 1105 76-77 0.07927681881376714 1105 78-79 0.1010701207184006 1105 80-81 0.1356426869472891 1105 82-83 0.13129513314340713 1105 84-85 0.16623118953610572 1105 86-87 0.2561910584047915 1105 88-89 0.3859336026413267 1105 90-91 0.4115533416609196 1105 92-93 0.30393490984637594 1105 94-95 0.03853491382503549 1105 96-97 0.052691443063849874 1105 98-99 0.2572891238627726 1105 100-101 0.03735588831389691 1106 1 0.05558643792276996 1106 2 0.14903409514809596 1106 3 0.08714462470149442 1106 4 0.10328461854145132 1106 5 0.005919318796074435 1106 6 0.15928548011170562 1106 7 0.12584875869218592 1106 8 0.09178222366741551 1106 9 0.1474911687730298 1106 10-11 0.2029975192311042 1106 12-13 0.17774916029720345 1106 14-15 0.1232271232351323 1106 16-17 0.0876868777623585 1106 18-19 0.11887765718801546 1106 20-21 0.1688126313127114 1106 22-23 0.21996271012988444 1106 24-25 0.28248070828981753 1106 26-27 0.30882614575860856 1106 28-29 0.2968057451044359 1106 30-31 0.20497095607894522 1106 32-33 0.27542802317133663 1106 34-35 0.11347662036247641 1106 36-37 0.19050196615339132 1106 38-39 0.2953993288110368 1106 40-41 0.127451379142542 1106 42-43 0.27854620048646694 1106 44-45 0.29461063056488257 1106 46-47 0.11877596573435767 1106 48-49 0.20079409921394387 1106 50-51 0.25196535858100333 1106 52-53 0.3294235835439281 1106 54-55 0.33925973155395894 1106 56-57 0.380905422896177 1106 58-59 0.25115609788525717 1106 60-61 0.24054365064444738 1106 62-63 0.27530760247432795 1106 64-65 0.2524116359726847 1106 66-67 0.1368652500306311 1106 68-69 0.23599330245734507 1106 70-71 0.2530193329561996 1106 72-73 0.32280968756352735 1106 74-75 0.20546521814296526 1106 76-77 0.3707000419552742 1106 78-79 0.4407340011117249 1106 80-81 0.3976754987682334 1106 82-83 0.3012773533889721 1106 84-85 0.34402061063298817 1106 86-87 0.4167660522108143 1106 88-89 0.4816673246719745 1106 90-91 0.5762417441027665 1106 92-93 0.5148834206523247 1106 94-95 0.5896618618809413 1106 96-97 0.7237121695257294 1106 98-99 0.7821909341281206 1106 100-101 0.54813898952548 1107 1 -0.06151477780987591 1107 2 -0.01260695070659068 1107 3 0.005437227513212406 1107 4 0.1011698388495148 1107 5 0.09188622428116844 1107 6 0.12316419281081892 1107 7 0.038642354648501964 1107 8 0.12602542429808494 1107 9 0.05509297575517991 1107 10-11 0.12103459018877061 1107 12-13 0.0747017134903345 1107 14-15 0.16252927491961344 1107 16-17 0.0659367212551345 1107 18-19 0.1040669536397445 1107 20-21 0.2231872980102949 1107 22-23 0.18793427991663947 1107 24-25 0.12614786573221437 1107 26-27 0.1265440192698506 1107 28-29 0.0747548435455414 1107 30-31 0.22614145620647008 1107 32-33 0.14924745634468195 1107 34-35 0.26711744208336086 1107 36-37 0.24196917305980037 1107 38-39 0.2673091806508623 1107 40-41 0.23077657796468287 1107 42-43 0.3532185014131457 1107 44-45 0.2345553313462574 1107 46-47 0.24360206882044366 1107 48-49 0.28240923442844945 1107 50-51 0.3909828989830544 1107 52-53 0.5222105327737623 1107 54-55 0.4893984626475003 1107 56-57 0.522978235466212 1107 58-59 0.5116691324250411 1107 60-61 0.39768752908663885 1107 62-63 0.2916186499520492 1107 64-65 0.4164017393060888 1107 66-67 0.529045299030912 1107 68-69 0.3598740825975426 1107 70-71 0.4304545660167136 1107 72-73 0.45592535992628314 1107 74-75 0.3966953069463237 1107 76-77 0.3327658793268782 1107 78-79 0.5811644539392908 1107 80-81 0.5664013947237798 1107 82-83 0.4815094284815231 1107 84-85 0.4780023862364082 1107 86-87 0.5015007048420692 1107 88-89 0.6537088551193939 1107 90-91 0.8716795585663561 1107 92-93 0.8756016535505289 1107 94-95 0.6883324199741061 1107 96-97 0.7310564158565036 1107 98-99 0.5395197229554363 1107 100-101 0.22477284764105576 1108 1 0.025752790716239815 1108 2 0.01403152709468003 1108 3 0.052003667102908935 1108 4 -0.008929328841986717 1108 5 -0.07251473929900243 1108 6 0.04735687199665506 1108 7 0.06036482329299986 1108 8 0.059523192679570514 1108 9 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-0.6715382390483065 2316 96-97 -0.7772449181435022 2316 98-99 -0.868340104515017 2316 100-101 -0.9084498340107565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 10.0 5 40.0 6 111.0 7 220.0 8 449.0 9 790.0 10 1541.0 11 2535.0 12 4213.0 13 6427.0 14 7480.0 15 10381.0 16 14697.0 17 23336.0 18 22291.0 19 11675.0 20 8970.0 21 8822.0 22 8929.0 23 9415.0 24 10297.0 25 11915.0 26 14038.0 27 16615.0 28 20138.0 29 24201.0 30 29765.0 31 36607.0 32 45467.0 33 59913.0 34 83931.0 35 127477.0 36 196598.0 37 338619.0 38 322614.0 39 35134.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.092559317702424 19.64776824551484 14.677647233297098 19.58202520348564 2 15.249387401248555 22.93191016010039 38.991498171770445 22.82720426688061 3 21.55615257634557 27.516339265567662 30.092552884063462 20.834955274023308 4 11.987406262824328 17.282080447378526 38.82437780155891 31.906135488238235 5 12.058727668350846 40.396549653817374 32.82183371886039 14.722888958971389 6 28.232258388017478 37.568566855405585 17.703477132945476 16.495697623631468 7 26.64840856279121 35.832903381597895 20.42642415189546 17.092263903715445 8 26.45687661066958 32.15236257928816 19.719675866974033 21.671084943068234 9 27.169628825195918 17.992127594720237 21.822898591649658 33.01534498843419 10-11 25.164052428160673 27.2078956292712 26.019480442364255 21.60857150020387 12-13 28.16819447981558 25.44191560982111 26.708216938470937 19.68167297189237 14-15 23.70686356270816 25.996235293496678 28.73201438848921 21.564886755305952 16-17 20.05735508159557 29.705617274746217 28.819658416279264 21.417369227378952 18-19 19.795001956226706 28.50740232226872 32.172968707630204 19.524627013874365 20-21 22.100695796061686 25.722879722156613 31.51380178059398 20.662622701187722 22-23 20.854418562302975 26.38905199930856 31.708533629263286 21.04799580912518 24-25 21.503246261628135 26.419367321241182 32.135532453874255 19.941853963256428 26-27 21.118762358501957 25.581612462450277 33.675660126268674 19.62396505277909 28-29 19.63806201069623 27.88574587939095 32.757769810658495 19.718422299254325 30-31 20.26342936525505 26.623250020041244 32.29203539531065 20.82128521939306 32-33 20.27823925197635 26.94395364702436 32.32271583467157 20.45509126632772 34-35 21.540750711813633 26.21598883533103 32.23177905568807 20.011481397167262 36-37 21.164556644573196 25.822367444128908 33.07929032509278 19.933785586205122 38-39 20.562156633065516 26.93214326959563 32.76877897480738 19.73692112253147 40-41 20.365368213889205 27.34533960866603 32.15312867566395 20.136163501780814 42-43 19.860858353579598 27.79121296781405 30.925050775120656 21.422877903485702 44-45 20.635641698633826 26.819719744685006 30.948761495310823 21.595877061370345 46-47 21.572877880326473 27.870710128370796 30.249658809413926 20.30675318188881 48-49 21.377634283599228 27.672897051357374 30.12970887007571 20.81975979496768 50-51 20.115104059453497 28.187793929029496 31.042567257295772 20.654534754221228 52-53 21.010244627384875 29.026422264704284 29.1147658877495 20.84856722016135 54-55 19.914737601885367 27.8571777308313 29.919989390858458 22.30809527642488 56-57 21.245861577093756 27.953372559006752 29.161679886308008 21.63908597759149 58-59 22.352646981878834 27.298432604569733 29.15996828985945 21.18895212369198 60-61 21.100333255361498 27.844259122952124 30.73435281782403 20.321054803862342 62-63 20.783706185068755 27.886468238350194 30.22505704860835 21.104768527972706 64-65 20.164636884059096 29.043040276424755 29.659579114676752 21.1327437248394 66-67 20.741995834657065 28.0196674389673 28.908588438319704 22.32974828805593 68-69 20.24846826945789 28.227256952004677 29.41942035067161 22.104854427865817 70-71 21.26995028942579 28.253644336989463 28.908930584324395 21.567474789260352 72-73 21.060564474946457 27.366438957190002 29.995071499544096 21.57792506831944 74-75 19.900651459350758 29.622497977797334 28.853335215884158 21.62351534696775 76-77 20.392071300116367 27.874980165026976 29.19159261610071 22.54135591875595 78-79 20.523161201593616 28.06794088820756 29.490941467819027 21.91795644237979 80-81 20.9375440207783 28.287244918661976 29.029647612741854 21.745563447817872 82-83 20.56526928408417 28.041647670629498 29.535214529312835 21.857868515973493 84-85 19.968185760323713 28.41195679676395 29.199758391236635 22.420099051675706 86-87 20.087618315437826 28.563894464379715 28.843736769905163 22.5047504502773 88-89 20.110802241481586 28.271264448524185 28.344395120249942 23.273538189744283 90-91 20.22189488241598 27.55223491105527 28.88994935800039 23.335920848528364 92-93 20.766998708165477 28.353887445453307 28.93052859651878 21.948585249862436 94-95 20.22974597439819 27.879675972537633 29.0695096702853 22.821068382778883 96-97 19.153756018774487 27.88957255979848 29.8377157620966 23.118955659330435 98-99 19.85705826368511 28.306092590484955 28.196568804524784 23.640280341305147 100-101 19.726620295152433 27.280602642747454 29.184495837615028 23.808281224485086 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2225.0 1 1536.0 2 1082.0 3 1401.0 4 1184.0 5 1094.0 6 2059.0 7 4087.0 8 6243.5 9 6871.5 10 5639.0 11 4459.0 12 4401.5 13 4587.5 14 4706.5 15 4754.5 16 4775.5 17 4996.0 18 5215.0 19 5317.5 20 5556.5 21 6113.0 22 6915.5 23 7873.5 24 8905.5 25 10200.0 26 12341.0 27 14605.0 28 16031.0 29 17601.5 30 20021.5 31 22731.0 32 25734.0 33 29015.0 34 32680.5 35 35331.5 36 37971.5 37 41110.0 38 41582.5 39 40651.0 40 40188.5 41 41216.0 42 42561.0 43 43698.5 44 45632.5 45 47075.0 46 47968.5 47 61908.0 48 98527.0 49 95949.5 50 60818.0 51 45956.0 52 38415.0 53 36385.0 54 35357.5 55 32264.0 56 28855.0 57 26177.5 58 24712.5 59 22495.0 60 19729.5 61 17157.0 62 14106.0 63 10320.0 64 7046.0 65 5523.5 66 4064.0 67 3110.5 68 2332.0 69 1501.0 70 1136.0 71 939.5 72 858.0 73 898.0 74 743.5 75 575.0 76 439.5 77 235.0 78 115.0 79 75.5 80 51.0 81 28.0 82 17.0 83 10.5 84 4.0 85 1.5 86 0.0 87 1.0 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34624859963290255 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011875904713018763 16-17 0.001847362955358474 18-19 6.597724840565979E-5 20-21 0.0 22-23 0.0 24-25 4.948293630424484E-4 26-27 0.0011216132228962163 28-29 0.0 30-31 0.0032658737960801596 32-33 0.0 34-35 0.011809927464613103 36-37 0.029359875540518606 38-39 0.023026059693575268 40-41 0.014284074279825344 42-43 0.011084177732150844 44-45 0.003694725910716948 46-47 0.0016824198343443246 48-49 6.927611082594278E-4 50-51 1.3195449681131959E-4 52-53 9.896587260848968E-5 54-55 0.0 56-57 0.0 58-59 0.004651396012599015 60-61 0.011183143604759334 62-63 0.005311168496655613 64-65 0.0023421923184009227 66-67 0.004783350509410335 68-69 0.004486452891584865 70-71 3.2988624202829897E-5 72-73 0.0 74-75 0.0 76-77 0.21073133140767736 78-79 2.3092036941980924E-4 80-81 0.0035957600381084586 82-83 4.618407388396185E-4 84-85 3.2988624202829897E-5 86-87 0.10602543818789528 88-89 0.3989974097332276 90-91 0.3866266756571664 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0014185108407216856 100-101 0.0010886245986933866 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1515674.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.11805032686406 #Duplication Level Percentage of deduplicated Percentage of total 1 74.28567701748541 32.7733923722259 2 13.664069318942632 12.056641957657401 3 4.97113585725036 6.579504657955497 4 2.273104013506444 4.011396690642958 5 1.2324661136755006 2.718700101464515 6 0.7500935912325868 1.9855600084714475 7 0.49176443439214274 1.5186981645832076 8 0.33966244288680053 1.1988195799540364 9 0.2666045431742621 1.0585865387819426 >10 1.48015875755113 12.374477463331118 >50 0.15006252378999366 4.578288003132499 >100 0.0839748441200103 6.7780023666611875 >500 0.004939678476832299 1.416604432742146 >1k 0.005089365703402975 5.295278067922067 >5k 7.484361328533787E-4 2.476531762261117 >10k+ 4.490616797120272E-4 3.179517832213107 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 20872 1.3770771287229313 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15330 1.0114312180587646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11944 0.7880322549572006 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9957 0.6569354623751545 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8141 0.5371207792704764 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7231 0.47708148322132593 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6457 0.42601509295534534 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5715 0.37705997463834573 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4821 0.31807631456368585 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4695 0.3097631812645727 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4455 0.2939286416472144 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4420 0.2916194379530163 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4396 0.29003598399128044 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3851 0.25407838361019586 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3802 0.25084549843831855 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3513 0.23177807364908287 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3291 0.21713112450302638 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2897 0.1911360886311964 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2725 0.17978800190542293 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2557 0.1687038241732721 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2499 0.1648771437657438 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2377 0.15682791946025332 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2142 0.14132326608492327 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2041 0.13465956399595164 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1983 0.13083288358842338 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1856 0.12245377304090457 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1806 0.11915491062062158 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1709 0.11275511752527258 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1529 0.10087921281225383 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.597724840565979E-5 0.0 16-17 0.0 0.0 0.0 1.3195449681131959E-4 0.0 18-19 0.0 0.0 0.0 1.6494312101414946E-4 0.0 20-21 0.0 0.0 0.0 2.6390899362263917E-4 0.0 22-23 0.0 0.0 0.0 0.0010226473502877268 0.0 24-25 0.0 0.0 0.0 0.0023751809426037526 0.0 26-27 0.0 0.0 0.0 0.003430816917094309 0.0 28-29 0.0 0.0 0.0 0.008016235681287665 0.0 30-31 0.0 0.0 0.0 0.018242709184164933 0.0 32-33 0.0 0.0 0.0 0.03958634904339588 0.0 34-35 0.0 0.0 0.0 0.07363060922071632 0.0 36-37 0.0 0.0 0.0 0.12443309049307437 0.0 38-39 0.0 0.0 0.0 0.2048923449237765 0.0 40-41 0.0 0.0 0.0 0.301186138971837 0.0 42-43 0.0 0.0 0.0 0.3896286404596239 0.0 44-45 0.0 0.0 0.0 0.4985570775773682 0.0 46-47 0.0 0.0 0.0 0.6375051627196877 0.0 48-49 0.0 0.0 0.0 0.792023878485743 0.0 50-51 0.0 0.0 0.0 0.976067412913331 0.0 52-53 0.0 0.0 0.0 1.1533482793793388 0.0 54-55 0.0 0.0 0.0 1.328847760138394 0.0 56-57 0.0 0.0 0.0 1.5556115629086467 0.0 58-59 0.0 0.0 0.0 1.8100528213850735 0.0 60-61 0.0 0.0 0.0 2.118364503184722 0.0 62-63 0.0 0.0 0.0 2.4135137239274407 0.0 64-65 0.0 0.0 0.0 2.696061290224679 0.0 66-67 0.0 0.0 0.0 3.029972144405723 0.0 68-69 0.0 0.0 0.0 3.418611126139262 0.0 70-71 0.0 0.0 0.0 3.857920634648348 0.0 72-73 0.0 0.0 0.0 4.297164165909027 0.0 74-75 0.0 0.0 0.0 4.744621864596213 0.0 76-77 0.0 0.0 0.0 5.202437991283086 0.0 78-79 0.0 0.0 0.0 5.758923092960623 0.0 80-81 0.0 0.0 0.0 6.38557499831758 0.0 82-83 0.0 0.0 0.0 6.99629339818457 0.0 84-85 0.0 0.0 0.0 7.609386978994164 0.0 86-87 0.0 0.0 0.0 8.22601034259346 0.0 88-89 0.0 0.0 0.0 8.88802605309585 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 60 0.003906393 31.74076 1 GGACCGT 120 1.5209662E-7 31.631863 6 CATTCCG 260 0.0 27.373726 9 GTAAGCG 225 0.0 25.30549 94-95 AGGACCG 170 1.1353404E-7 25.119423 5 GTACATG 14775 0.0 24.135868 1 TACATGG 14780 0.0 23.756 2 TACCTGG 1155 0.0 23.415794 2 CATGGGG 4085 0.0 23.230255 4 GAGTACT 11310 0.0 22.738022 12-13 GGACCGA 105 0.002210624 22.594189 6 AGTACTT 11355 0.0 22.564342 12-13 ACATGGG 14760 0.0 22.438047 3 TAGACCG 110 0.002896205 21.56718 4 GTACTTT 12060 0.0 21.166594 14-15 GTCGGGA 550 0.0 20.704493 2 CATGGGT 1380 0.0 20.285652 4 GATCGTC 120 0.004794587 19.769915 7 ATGGGAG 2675 0.0 19.688618 5 GACCGGT 145 6.192968E-4 19.63357 6 >>END_MODULE