##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939166_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1489093 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.454107298872536 34.0 31.0 34.0 31.0 34.0 2 32.63709855596662 34.0 31.0 34.0 31.0 34.0 3 32.73542216637913 34.0 31.0 34.0 31.0 34.0 4 36.18722940743123 37.0 35.0 37.0 35.0 37.0 5 36.17344383460267 37.0 35.0 37.0 35.0 37.0 6 36.257614534485086 37.0 37.0 37.0 35.0 37.0 7 36.21490665794548 37.0 37.0 37.0 35.0 37.0 8 36.20909775279314 37.0 37.0 37.0 35.0 37.0 9 37.91540958153722 39.0 38.0 39.0 35.0 39.0 10-11 37.83663612682351 39.0 38.0 39.0 35.0 39.0 12-13 37.756362430016125 39.0 38.0 39.0 35.0 39.0 14-15 39.04544813520714 40.0 38.0 41.0 36.0 41.0 16-17 39.1860165886214 40.5 38.5 41.0 36.0 41.0 18-19 39.30013269822637 41.0 39.0 41.0 36.0 41.0 20-21 39.28550399471356 41.0 39.0 41.0 36.0 41.0 22-23 39.203027950571254 41.0 39.0 41.0 35.5 41.0 24-25 39.106495699059764 41.0 39.0 41.0 35.0 41.0 26-27 38.92395102253519 40.0 38.0 41.0 35.0 41.0 28-29 38.77474442496204 40.0 38.0 41.0 35.0 41.0 30-31 38.53677977131046 40.0 38.0 41.0 35.0 41.0 32-33 38.31725352278199 40.0 38.0 41.0 34.0 41.0 34-35 38.13494825373566 40.0 37.0 41.0 34.0 41.0 36-37 37.93511016437523 40.0 37.0 41.0 33.5 41.0 38-39 37.77053212928944 40.0 37.0 41.0 33.0 41.0 40-41 37.57203277431296 40.0 37.0 41.0 33.0 41.0 42-43 37.29292058991615 40.0 36.0 41.0 33.0 41.0 44-45 36.89409425737681 40.0 36.0 41.0 31.5 41.0 46-47 36.36552854657164 40.0 36.0 41.0 30.5 41.0 48-49 35.837396992666 39.5 35.0 41.0 28.5 41.0 50-51 35.50668326289896 39.0 35.0 41.0 27.0 41.0 52-53 35.25168844390512 39.0 35.0 41.0 26.5 41.0 54-55 35.02129551344342 39.0 35.0 40.0 26.0 41.0 56-57 34.78993118629931 38.5 35.0 40.0 25.0 41.0 58-59 34.47748394492486 38.0 34.5 40.0 24.0 41.0 60-61 34.06529142236247 38.0 34.0 40.0 22.5 41.0 62-63 33.70450166645065 37.0 34.0 40.0 22.0 41.0 64-65 33.27144543692033 37.0 33.0 39.5 20.5 41.0 66-67 32.83438643523272 36.0 33.0 39.0 18.5 40.5 68-69 32.338341527359276 35.5 32.5 39.0 15.0 40.0 70-71 31.80591171941578 35.0 31.5 38.0 10.0 40.0 72-73 31.230359017200403 35.0 31.0 37.0 7.5 39.0 74-75 30.711339385787184 35.0 31.0 37.0 3.5 39.0 76-77 28.740852653259402 32.5 28.0 35.0 2.0 37.0 78-79 29.70163347755983 34.0 29.5 35.5 2.0 37.5 80-81 29.688870674967916 34.0 30.0 35.0 2.0 37.0 82-83 29.30112994957333 34.0 29.5 35.0 2.0 37.0 84-85 28.844660138755607 34.0 29.0 35.0 2.0 36.0 86-87 28.190753028857163 34.0 28.5 35.0 2.0 36.0 88-89 27.503806343861665 33.0 26.5 35.0 2.0 35.0 90-91 26.92545965900048 33.0 25.5 35.0 2.0 35.0 92-93 26.50606510137379 33.0 25.0 34.5 2.0 35.0 94-95 25.98026046727773 32.0 24.5 34.0 2.0 35.0 96-97 25.168813499224022 32.0 20.5 34.0 2.0 35.0 98-99 24.04220522156776 32.0 2.0 34.0 2.0 35.0 100-101 18.38272458469686 24.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19195419582774065 1101 2 0.21945473299285823 1101 3 0.15295122034545017 1101 4 0.16868559389348547 1101 5 0.1527772018207898 1101 6 0.16281050710064449 1101 7 0.21576882561179644 1101 8 0.18091540101296744 1101 9 0.17446808329920316 1101 10-11 0.24117485968539398 1101 12-13 0.29664451024733296 1101 14-15 0.39068332882878565 1101 16-17 0.32089759936746276 1101 18-19 0.30396292544239856 1101 20-21 0.34127012057935247 1101 22-23 0.41040915362824393 1101 24-25 0.3237057968538508 1101 26-27 0.3623462708417122 1101 28-29 0.37468931683243767 1101 30-31 0.4002493019491382 1101 32-33 0.4239663537636176 1101 34-35 0.3679989775075754 1101 36-37 0.42685195582851776 1101 38-39 0.425061091054431 1101 40-41 0.42091474828695397 1101 42-43 0.32409278555435606 1101 44-45 0.36598067579610927 1101 46-47 0.3080681039998794 1101 48-49 0.22513809060619394 1101 50-51 0.22641837398084874 1101 52-53 0.17682508734669966 1101 54-55 0.1983370618105198 1101 56-57 0.1837567082976861 1101 58-59 0.21474851358053115 1101 60-61 0.2647156710167806 1101 62-63 0.25018370917013755 1101 64-65 0.20590772345016006 1101 66-67 0.11123179628355473 1101 68-69 0.3392390723182217 1101 70-71 0.2921253189780906 1101 72-73 0.41918032811973305 1101 74-75 0.1963399775278063 1101 76-77 0.03956139721348251 1101 78-79 0.13740556921436564 1101 80-81 0.26105801391799943 1101 82-83 0.1603747015610857 1101 84-85 0.3593797652222577 1101 86-87 0.3352085460641163 1101 88-89 0.21604953223796386 1101 90-91 0.5601150838230069 1101 92-93 0.43070211977481065 1101 94-95 0.3786701388601301 1101 96-97 0.5479095577834876 1101 98-99 0.5175128501313573 1101 100-101 -0.23602868413949096 1102 1 0.07514528887876537 1102 2 0.09243578356395687 1102 3 0.08533755195533388 1102 4 0.07730058946102503 1102 5 0.13973409673806003 1102 6 0.11130615918543896 1102 7 0.1450229672764607 1102 8 0.1750846596895883 1102 9 0.20032242173456893 1102 10-11 0.1282459610376847 1102 12-13 0.14336032261826404 1102 14-15 0.1800499603090273 1102 16-17 0.18272158438419694 1102 18-19 0.12345718885892865 1102 20-21 0.17958538670660573 1102 22-23 0.11954528411181542 1102 24-25 0.24446683574168304 1102 26-27 0.20620756286686515 1102 28-29 0.20713156667998334 1102 30-31 0.17674384976083246 1102 32-33 0.2167072953934337 1102 34-35 0.31722771576666986 1102 36-37 0.3021533250246833 1102 38-39 0.3318928521009994 1102 40-41 0.22424300435533695 1102 42-43 0.10611766628774433 1102 44-45 0.38922869912163094 1102 46-47 0.38037173042249606 1102 48-49 0.3574622267879235 1102 50-51 0.26654018990799955 1102 52-53 0.39688352927115744 1102 54-55 0.40992866267343686 1102 56-57 0.46031306721975085 1102 58-59 0.1801810504366017 1102 60-61 0.07928266016876506 1102 62-63 0.15776969386917017 1102 64-65 0.3023820561463779 1102 66-67 0.41613991871183487 1102 68-69 0.28504449991019243 1102 70-71 0.3612708140050209 1102 72-73 0.5216554627590604 1102 74-75 0.39134378227387145 1102 76-77 0.3210885864156907 1102 78-79 0.33943246121081927 1102 80-81 0.5916629044899153 1102 82-83 0.670442866725498 1102 84-85 0.3455446452382489 1102 86-87 0.5571860147934586 1102 88-89 0.38344747024494197 1102 90-91 0.8735963752332516 1102 92-93 0.5756892066972448 1102 94-95 0.41457983186708347 1102 96-97 0.45117036608064964 1102 98-99 0.44872541117383236 1102 100-101 -0.5070095528001701 1103 1 0.19566410005005963 1103 2 0.17479942037403617 1103 3 0.12478981563094038 1103 4 0.16185939493568213 1103 5 0.21661472277855864 1103 6 0.15923830539251327 1103 7 0.14535383861600337 1103 8 0.1230028098548388 1103 9 0.2412817153518816 1103 10-11 0.27273943937328227 1103 12-13 0.15612219592706822 1103 14-15 0.12302437701784186 1103 16-17 0.2956232953389204 1103 18-19 0.311576882026408 1103 20-21 0.3419459173775863 1103 22-23 0.41343085542042246 1103 24-25 0.32214578864132193 1103 26-27 0.33260593932601523 1103 28-29 0.3117074743355772 1103 30-31 0.36181541373256465 1103 32-33 0.376274142453525 1103 34-35 0.42220631655535357 1103 36-37 0.45395236887403456 1103 38-39 0.4427028300510969 1103 40-41 0.4747037073986107 1103 42-43 0.304020162985168 1103 44-45 0.5452864070119645 1103 46-47 0.7010413059741083 1103 48-49 0.7867745423225472 1103 50-51 0.6704146994326763 1103 52-53 0.7492848326218606 1103 54-55 0.7379544473111963 1103 56-57 0.657729692553076 1103 58-59 0.7515492003563082 1103 60-61 0.7388685369348451 1103 62-63 0.6492176826134042 1103 64-65 0.6164572247127396 1103 66-67 0.5635826945443512 1103 68-69 0.4727013415191976 1103 70-71 0.6941313716859021 1103 72-73 0.7809781771072117 1103 74-75 0.82402742507524 1103 76-77 0.6568023722713399 1103 78-79 0.5803669548242034 1103 80-81 0.7265669456628316 1103 82-83 0.6259676814621038 1103 84-85 0.6036503791955923 1103 86-87 0.4553912065883381 1103 88-89 0.607164990833855 1103 90-91 0.7348707393955252 1103 92-93 0.37958440335390975 1103 94-95 0.5921236124680789 1103 96-97 0.39155827742922966 1103 98-99 0.4538784713960986 1103 100-101 -0.22787415214505558 1104 1 0.013016007519432549 1104 2 0.04560723743792039 1104 3 0.07462350953466057 1104 4 0.027917460413227957 1104 5 0.09376437706096397 1104 6 0.08497631630445568 1104 7 0.09526881547098043 1104 8 0.07394324771077976 1104 9 0.06999733281075038 1104 10-11 0.0304823767388811 1104 12-13 0.03789467081034559 1104 14-15 -0.01859349533611976 1104 16-17 0.06399338957413647 1104 18-19 0.13344499984899016 1104 20-21 0.18577751323874736 1104 22-23 0.15734155490734025 1104 24-25 0.12043301549673657 1104 26-27 0.13173721305930997 1104 28-29 0.1562391048892522 1104 30-31 0.09803927876045293 1104 32-33 0.16317144851269916 1104 34-35 0.1560794550726996 1104 36-37 0.14836469608150793 1104 38-39 0.09246578422695251 1104 40-41 0.10870925466553416 1104 42-43 0.06847984045677435 1104 44-45 0.03223607578393484 1104 46-47 -0.058407689857482126 1104 48-49 -0.20815558084468933 1104 50-51 -0.16157962489690192 1104 52-53 -0.31597830915269043 1104 54-55 -0.22758423188797394 1104 56-57 -0.26988283279630565 1104 58-59 -0.1392506886747924 1104 60-61 -0.2864481573464346 1104 62-63 -0.1428204756091631 1104 64-65 -0.24462196549702497 1104 66-67 -0.10859756748125449 1104 68-69 -0.226196273179589 1104 70-71 -0.37633063943101774 1104 72-73 -0.6085108770831873 1104 74-75 -0.6317122409460723 1104 76-77 -0.6899456912212472 1104 78-79 -0.39296281305407277 1104 80-81 -0.2579627882886477 1104 82-83 -0.19934730469917739 1104 84-85 -0.5617212576486743 1104 86-87 -0.516319675844116 1104 88-89 -0.6598910290227415 1104 90-91 -0.3630884695210952 1104 92-93 -0.5605801533918999 1104 94-95 -0.5376917397609873 1104 96-97 -0.5566795022975057 1104 98-99 -0.7324550372649519 1104 100-101 -1.3590368576421987 1105 1 -0.008345718989353657 1105 2 0.05414428862202669 1105 3 0.0983152589616978 1105 4 0.042938468816586806 1105 5 0.035542451927270236 1105 6 0.09133613295838927 1105 7 0.030687073683353105 1105 8 -0.030048348927877555 1105 9 0.006275025706095505 1105 10-11 0.10106583739586483 1105 12-13 0.09721476248337524 1105 14-15 0.007963036367478082 1105 16-17 0.12976401600652565 1105 18-19 0.11687700901629228 1105 20-21 0.23010524433118462 1105 22-23 0.16965534405936467 1105 24-25 0.11288431419803402 1105 26-27 0.16235218632133552 1105 28-29 0.12794460527121743 1105 30-31 0.11090692581927897 1105 32-33 0.20421900389849412 1105 34-35 0.27651490197873585 1105 36-37 0.3209009833236749 1105 38-39 0.20581566963565479 1105 40-41 0.3012990178435331 1105 42-43 0.23329076482652056 1105 44-45 0.18421277555475513 1105 46-47 0.13036236361845255 1105 48-49 0.0838087048695968 1105 50-51 0.0845338204812478 1105 52-53 0.024281431107048945 1105 54-55 -0.012448632193553522 1105 56-57 -0.14293955548537696 1105 58-59 -0.2152151653745591 1105 60-61 -0.21001576620815143 1105 62-63 -0.2300721944785309 1105 64-65 -0.17236738184538325 1105 66-67 -0.14378186847437746 1105 68-69 -0.17003699128501637 1105 70-71 -0.1544771252980901 1105 72-73 -0.40554105278983243 1105 74-75 -0.24606957478869873 1105 76-77 -0.4733920242999332 1105 78-79 -0.5025120873092241 1105 80-81 -0.33772310150483875 1105 82-83 -0.40630872563118814 1105 84-85 -0.48465861440956104 1105 86-87 -0.7072669638502305 1105 88-89 -0.8727252536216739 1105 90-91 -0.5813610058083398 1105 92-93 -0.4388316812757793 1105 94-95 -0.4217540010291323 1105 96-97 -0.7947667826641975 1105 98-99 -0.8409968630861862 1105 100-101 -1.4589461395367742 1106 1 -0.033513193788380136 1106 2 0.051535935085389895 1106 3 0.06589479619958638 1106 4 0.08808382630882505 1106 5 0.10346969874790801 1106 6 0.03680102173385791 1106 7 0.09351776995318062 1106 8 0.08161746707314421 1106 9 0.0656871094115914 1106 10-11 0.05793788664940536 1106 12-13 0.05330552850779213 1106 14-15 0.06984949377039129 1106 16-17 0.058126006138806474 1106 18-19 -0.04019574416307137 1106 20-21 0.1352996214312725 1106 22-23 0.22338524708347762 1106 24-25 0.1592984926676877 1106 26-27 0.2236883693845897 1106 28-29 0.25870095336360777 1106 30-31 0.2798477939235724 1106 32-33 0.2854195589578197 1106 34-35 0.23005367106554075 1106 36-37 0.2916104384723255 1106 38-39 0.3163118203736133 1106 40-41 0.44045090419459854 1106 42-43 0.38923851268921794 1106 44-45 0.38752983990761436 1106 46-47 0.27466077712701775 1106 48-49 0.3276118714724703 1106 50-51 0.38577207066201424 1106 52-53 0.42951302482914144 1106 54-55 0.485269988877441 1106 56-57 0.5537255693498011 1106 58-59 0.4228611606005117 1106 60-61 0.3720330619999217 1106 62-63 0.34125771492557533 1106 64-65 0.6055432238285618 1106 66-67 0.46144839608263055 1106 68-69 0.3082821997319627 1106 70-71 0.43713026060637716 1106 72-73 0.4832955556062366 1106 74-75 0.26995191515218053 1106 76-77 0.23213278323696684 1106 78-79 0.38696943197961886 1106 80-81 0.5625243890315055 1106 82-83 0.5240148463138574 1106 84-85 0.5375171756461583 1106 86-87 0.4337967935399263 1106 88-89 0.39527270478077625 1106 90-91 0.659150084745022 1106 92-93 0.658803202395692 1106 94-95 0.45041275070409625 1106 96-97 0.006048527190763764 1106 98-99 0.1894588278669822 1106 100-101 -0.6706621979775029 1107 1 0.011568453454501082 1107 2 0.009573115057328607 1107 3 0.03398039657638208 1107 4 0.028267353369699322 1107 5 0.0986817478714812 1107 6 0.11965507521419028 1107 7 0.09834399177874786 1107 8 0.08687742632223916 1107 9 0.04289599378280684 1107 10-11 0.07821230466308293 1107 12-13 0.0029123838043148 1107 14-15 0.0473165794784407 1107 16-17 0.049656134815940334 1107 18-19 0.21577847856671184 1107 20-21 0.21333306615857595 1107 22-23 0.08371388737788976 1107 24-25 0.05614705860419633 1107 26-27 0.041941417920831725 1107 28-29 0.06535535291536831 1107 30-31 0.03589473145670752 1107 32-33 -0.0037641523138916 1107 34-35 -0.009763067218820254 1107 36-37 0.06500202529303323 1107 38-39 -0.09406705692003214 1107 40-41 0.014317407980108499 1107 42-43 0.006756350778616138 1107 44-45 0.04928864005722744 1107 46-47 -0.10078463293599071 1107 48-49 -0.2147323823486289 1107 50-51 -0.20771212165001884 1107 52-53 -0.09637114890872311 1107 54-55 -0.2736202250367512 1107 56-57 -0.19332743999698465 1107 58-59 -0.2005118775668535 1107 60-61 -0.1844875167997131 1107 62-63 -0.26382359553295487 1107 64-65 -0.3927358591586696 1107 66-67 -0.37596538584745076 1107 68-69 -0.3890275056276522 1107 70-71 -0.2845896766700875 1107 72-73 -0.5460590277991777 1107 74-75 -0.5966728065542348 1107 76-77 -0.6406091669791643 1107 78-79 -0.4403716912778428 1107 80-81 -0.20391591772801476 1107 82-83 -0.343244152589552 1107 84-85 -0.4032054615181977 1107 86-87 -0.5634482084016845 1107 88-89 -0.6308472180037583 1107 90-91 -0.11289640578343096 1107 92-93 0.08441570342263205 1107 94-95 0.20272138192574118 1107 96-97 -0.2011958062849395 1107 98-99 -0.097360504167181 1107 100-101 -1.0030469220516167 1108 1 -0.05520071355391565 1108 2 -0.02178174914420339 1108 3 0.051694793527069294 1108 4 0.00636551314561018 1108 5 0.009392453940400003 1108 6 0.06944439885201348 1108 7 -0.00340571127457423 1108 8 -0.007496309631662257 1108 9 0.039214289707381056 1108 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0.5456754124738623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 21.0 5 54.0 6 141.0 7 202.0 8 443.0 9 848.0 10 1488.0 11 2573.0 12 4275.0 13 6262.0 14 7466.0 15 10612.0 16 14948.0 17 23559.0 18 22969.0 19 12293.0 20 9452.0 21 9694.0 22 10095.0 23 10586.0 24 12141.0 25 13444.0 26 15911.0 27 18628.0 28 22430.0 29 27302.0 30 33384.0 31 40669.0 32 51756.0 33 67538.0 34 96116.0 35 145398.0 36 223060.0 37 341921.0 38 220798.0 39 10609.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.16365690390679 19.643144991316372 14.628694757785759 19.56450334699108 2 15.338665885878184 22.94369794230448 38.899920958596944 22.817715213220396 3 21.51819933341974 27.503050514642137 30.079383893417 20.899366258521127 4 11.987968515062525 17.301807207474617 38.807247096051086 31.902977181411774 5 12.101393264221912 40.3633621271472 32.84039344755499 14.694851161075903 6 28.28399569402314 37.58972743811166 17.6559153793618 16.470361488503404 7 26.615799013224827 35.8019277506509 20.47273071594588 17.10954252017839 8 26.466312043639988 32.182879108289406 19.75047898284392 21.600329865226687 9 27.091524840960236 18.003778138773065 21.77795476843958 33.12674225182712 10-11 25.17250433653237 27.236713892282083 25.972924458042577 21.617857313142967 12-13 28.13554962651762 25.465434328144713 26.66989234386301 19.729123701474656 14-15 23.661941374500614 26.041600210599974 28.704521385366387 21.59193702953302 16-17 20.081716064211044 29.7632027217198 28.72496278688849 21.430118427180663 18-19 19.820823816914054 28.513564968742717 32.11965270134236 19.545958513000866 20-21 22.134514096836128 25.73076362591188 31.482721361258157 20.652000915993828 22-23 20.835669766764063 26.36198679330304 31.67411303390722 21.12823040602568 24-25 21.54053559466588 26.411824651591488 32.07305791950466 19.974581834237966 26-27 21.158105469012334 25.59172193702805 33.59165343670167 19.658519157257945 28-29 19.618425316090168 27.905217439480758 32.72624098234328 19.7501162620858 30-31 20.22284706193636 26.654211657700362 32.31043997923568 20.812501301127597 32-33 20.304910438770445 26.870719290198796 32.37504306312635 20.449327207904407 34-35 21.52730367117186 26.24316488924963 32.22085395941935 20.00867748015916 36-37 21.196108514488614 25.86391623216716 33.02613848722968 19.913836766114546 38-39 20.525389740126233 26.952092669134238 32.74985802138058 19.77265956935895 40-41 20.323801343010278 27.419686850326247 32.17344759140304 20.08306421526043 42-43 19.84931077662675 27.777772181024524 30.916912727923577 21.45600431442515 44-45 20.678245318187937 26.789524883086912 30.900715128303023 21.63151467042212 46-47 21.589526237461516 27.858229864554847 30.22385253079886 20.32839136718477 48-49 21.374720961905762 27.628532132025647 30.20887898544301 20.787867920625583 50-51 20.10373508770339 28.20245231708271 31.01814776424259 20.675664830971307 52-53 21.0065455952046 29.11483701823862 29.060642955141148 20.817974431415635 54-55 19.891571580821346 27.872302132909095 29.897864001778263 22.338262284491297 56-57 21.269027948230214 27.88073944365444 29.190127544125026 21.66010506399032 58-59 22.317755088815233 27.325765097716737 29.115903871834487 21.240575941633544 60-61 21.07588008542302 27.81936255087102 30.798423166292828 20.306334197413133 62-63 20.738734521835223 27.859941097601965 30.255423926354208 21.145900454208608 64-65 20.15037785861983 29.00841409517081 29.698854772408428 21.14235327380093 66-67 20.703997636104475 27.966562966420263 28.924142122502843 22.405297274972426 68-69 20.232541115775604 28.185687700760294 29.38576667850885 22.19600450495525 70-71 21.262568909489076 28.236655785307953 28.942589808144703 21.558185497058272 72-73 21.031157892757538 27.39113675237208 29.959747309268124 21.617958045602258 74-75 19.872029483719285 29.609332660888203 28.872172523811475 21.646465331581037 76-77 20.415706840771858 27.876872962855664 29.143507050897266 22.56391314547521 78-79 20.52969034510505 28.09733814867975 29.421815883523607 21.951155622691594 80-81 20.88443425923594 28.30683220428618 28.99997481679216 21.808758719685713 82-83 20.5694338768633 28.007384360815614 29.477373139219647 21.945808623101446 84-85 19.962587964620074 28.41011273305294 29.18662568422523 22.44067361810176 86-87 20.09816065580273 28.521146290033446 28.798263078759454 22.582429975404374 88-89 20.025566288915925 28.211701820594925 28.331238214959146 23.431493675530007 90-91 20.208579850230134 27.48425804497952 28.848184258685684 23.458977846104663 92-93 20.725972118598367 28.29299446038629 28.8271451145093 22.15388830650604 94-95 20.19427262098472 27.780568439983266 28.98072853743856 23.044430401593452 96-97 19.11025704908961 27.860550012658713 29.71909746402676 23.310095474224916 98-99 19.79241026908362 28.276042229789848 28.181017815566676 23.750529685559847 100-101 19.955751484380222 26.79096857509236 29.188742516819843 24.064537423707574 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2018.0 1 1423.0 2 1110.5 3 1404.5 4 1197.5 5 1090.0 6 1929.5 7 3882.5 8 5904.0 9 6450.0 10 5304.0 11 4211.0 12 4184.5 13 4411.0 14 4604.5 15 4684.5 16 4767.5 17 4976.5 18 5151.0 19 5258.5 20 5479.0 21 6025.0 22 6618.0 23 7524.5 24 8810.0 25 10122.5 26 12068.5 27 14323.0 28 15657.0 29 17181.0 30 19660.5 31 22160.0 32 25134.0 33 28620.5 34 32519.0 35 34951.5 36 37315.0 37 40479.5 38 41029.0 39 40077.0 40 39617.0 41 40705.5 42 41960.0 43 43035.0 44 44858.0 45 46287.0 46 47196.0 47 60948.5 48 96069.0 49 93470.5 50 60090.0 51 45769.5 52 38257.5 53 36011.0 54 34753.0 55 31819.5 56 28351.5 57 25673.0 58 24277.0 59 22003.5 60 19310.5 61 16686.0 62 13654.5 63 10065.5 64 7048.5 65 5483.5 66 3941.5 67 3065.0 68 2389.5 69 1549.5 70 1172.0 71 966.5 72 826.0 73 858.0 74 704.5 75 586.5 76 450.0 77 210.0 78 112.5 79 64.5 80 35.0 81 20.5 82 13.5 83 7.0 84 3.5 85 2.0 86 1.5 87 1.5 88 2.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4310006158111011 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.40169620030448E-4 16-17 0.002316846563646461 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.357748642965886E-5 26-27 0.003290593670106568 28-29 3.357748642965886E-5 30-31 0.0 32-33 0.0 34-35 0.012188627573966167 36-37 0.027332073953742313 38-39 0.020985929018536787 40-41 0.008360794120985057 42-43 0.009032343849578234 44-45 0.003995720885129404 46-47 0.0051709329101674644 48-49 0.003290593670106568 50-51 7.722821878821537E-4 52-53 0.0 54-55 0.0 56-57 3.357748642965886E-5 58-59 0.0012423669978973778 60-61 0.002216114104357485 62-63 0.0013095219707566954 64-65 0.0029548188058099796 66-67 0.0017124518079126017 68-69 0.00443222820871497 70-71 1.3430994571863545E-4 72-73 0.0 74-75 0.0 76-77 0.09468851173163799 78-79 0.016251503431954886 80-81 3.6935235072624745E-4 82-83 0.0 84-85 0.0 86-87 0.1096976481656955 88-89 0.42180038452937463 90-91 0.42653481011595645 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.3430994571863545E-4 100-101 2.35042405007612E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1489093.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.77210759459858 #Duplication Level Percentage of deduplicated Percentage of total 1 74.89989906731982 33.53426339866617 2 13.341146481063875 11.946224913709843 3 4.8492563448041555 6.513342804700844 4 2.2191343912710377 3.9742129493144374 5 1.2413069473407616 2.7787964102131646 6 0.7248776698278389 1.9472580615872341 7 0.45684166150407124 1.4317634815788824 8 0.333999537651784 1.1963090589033483 9 0.2522252974439831 1.0163392339717479 >10 1.4438418557995154 12.282538868443337 >50 0.14628426998247948 4.534447576925955 >100 0.0803881040728592 6.6894326581174255 >500 0.0044993216324135 1.3367831287524545 >1k 0.005099231183401966 5.227004676083852 >5k 7.498869387355833E-4 2.4468073351723274 >10k+ 4.4993216324135E-4 3.144475443859187 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 20540 1.3793631425303858 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14616 0.9815370833117877 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11673 0.7838999981868158 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9516 0.6390467217292674 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7823 0.5253533526784425 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7201 0.4835829595599469 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6334 0.42535959809091844 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5565 0.3737174239621031 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4769 0.3202620655660862 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4721 0.31703862686883894 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4455 0.29917540408826043 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4237 0.28453562000492916 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4163 0.2795661520133397 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3825 0.2568677711868903 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3569 0.23967609813490492 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3378 0.22684949831877524 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3230 0.21691056233559625 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2830 0.19004857319186913 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2569 0.17252112527558722 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2393 0.1607018500523473 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2285 0.15344911298354097 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2281 0.15318049309210371 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2016 0.13538442528438452 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1968 0.13216098658713726 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1904 0.12786306832414093 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1822 0.12235636054967688 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1812 0.12168481082108369 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1655 0.11114148008217083 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.715497285931772E-5 0.0 14-15 0.0 0.0 0.0 6.715497285931772E-5 0.0 16-17 0.0 0.0 0.0 1.3430994571863545E-4 0.0 18-19 0.0 0.0 0.0 2.686198914372709E-4 0.0 20-21 0.0 0.0 0.0 4.70084810015224E-4 0.0 22-23 0.0 0.0 0.0 0.001007324592889766 0.0 24-25 0.0 0.0 0.0 0.0018467617536312374 0.0 26-27 0.0 0.0 0.0 0.0029883962922396387 0.0 28-29 0.0 0.0 0.0 0.007185582095946996 0.0 30-31 0.0 0.0 0.0 0.019273477210624185 0.0 32-33 0.0 0.0 0.0 0.040024363824153364 0.0 34-35 0.0 0.0 0.0 0.07108353877158781 0.0 36-37 0.0 0.0 0.0 0.1257812641655021 0.0 38-39 0.0 0.0 0.0 0.2119410943440067 0.0 40-41 0.0 0.0 0.0 0.3150575551694891 0.0 42-43 0.0 0.0 0.0 0.4134395904083895 0.0 44-45 0.0 0.0 0.0 0.53149803269507 0.0 46-47 0.0 0.0 0.0 0.671851925971044 0.0 48-49 0.0 0.0 0.0 0.8325201985369618 0.0 50-51 0.0 0.0 0.0 1.019916150300888 0.0 52-53 0.0 0.0 0.0 1.1977760959187909 0.0 54-55 0.0 0.0 0.0 1.3807062419875722 0.0 56-57 0.0 0.0 0.0 1.6098725868699941 0.0 58-59 0.0 0.0 0.0 1.8662031182740098 0.0 60-61 0.0 0.0 0.0 2.1724297945124986 0.0 62-63 0.0 0.0 0.0 2.4717059310600478 0.0 64-65 0.0 0.0 0.0 2.7507348432905134 0.0 66-67 0.0 0.0 0.0 3.082043901891957 0.0 68-69 0.0 0.0 0.0 3.4808437082170154 0.0 70-71 0.0 0.0 0.0 3.9197350333390863 0.0 72-73 0.0 0.0 0.0 4.3580555411918525 0.0 74-75 0.0 0.0 0.0 4.810612903290796 0.0 76-77 0.0 0.0 0.0 5.2675017611391635 0.0 78-79 0.0 0.0 0.0 5.8152512972661885 0.0 80-81 0.0 0.0 0.0 6.436300486269158 0.0 82-83 0.0 0.0 0.0 7.0552007161406305 0.0 84-85 0.0 0.0 0.0 7.666243814187562 0.0 86-87 0.0 0.0 0.0 8.28652072100265 0.0 88-89 0.0 0.0 0.0 8.96565896152893 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 360 0.0 36.905846 94-95 TCACGCA 95 3.5165314E-5 29.968655 4 TAGGACG 80 4.521869E-4 29.656483 4 TATCACG 100 4.9895054E-5 28.470222 2 ATCACGC 100 4.9895054E-5 28.470222 3 TACCTGG 1050 0.0 27.114496 2 GGGACCG 120 1.7233178E-4 23.725185 5 TACATGG 14685 0.0 22.812368 2 GTACATG 14830 0.0 22.718199 1 AGTACTT 10985 0.0 22.636776 12-13 GAGTACT 10945 0.0 22.285929 12-13 CATGGGG 4210 0.0 22.203617 4 ACATGGG 14740 0.0 21.72931 3 GTCGGGA 525 0.0 21.691597 2 CGCGGAA 125 8.998541E-9 20.999445 82-83 GGGAACG 205 6.900991E-7 20.831871 7 GGAACGC 115 0.0037464085 20.630596 8 GTACTTT 11710 0.0 20.586863 14-15 TATAACG 140 4.8869924E-4 20.335873 2 GTGTAGC 570 0.0 20.06467 1 >>END_MODULE