FastQCFastQC Report
Fri 27 May 2016
SRR939165_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939165_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036997
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107740.5289158501460728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81820.40166971281744646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55770.2737853811272182No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53060.2604814832815168No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA37350.18335814927562485No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA33230.16313229720024133No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30710.15076114495995824No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT21650.10628390714370221No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20870.10245474097409078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG20250.030.881352
GTACCTG24200.025.8929881
GTACATG108750.023.178691
TACATGG108400.023.0318452
GAGTACT83950.022.28248812-13
ACATGGG108900.021.9255283
GTGTAGC11100.020.9554391
GTACTTT90950.020.38626914-15
ACCTGGG29500.020.2346613
CATGGGG44800.019.8804954
AGTACTT87150.019.86128212-13
ACCGCGC1951.060015E-519.4296078
GTATCAA209400.018.951921
ATGGGAG24250.017.9731225
ACTTTTT106400.017.80591816-17
CATGGGA63200.017.765554
TACTTTT102050.016.79980314-15
TATCAAC234450.016.771632
TCAACGC234550.016.7038844
CAACGCA235250.016.6944585
Produced by FastQC (version 0.11.5)