##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939165_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2036997 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.973263583598797 33.0 31.0 34.0 30.0 34.0 2 32.06722788496988 34.0 31.0 34.0 30.0 34.0 3 32.11571249245826 34.0 31.0 34.0 30.0 34.0 4 35.5632129060573 37.0 35.0 37.0 33.0 37.0 5 35.612752988836014 37.0 35.0 37.0 33.0 37.0 6 35.69912179546656 37.0 36.0 37.0 35.0 37.0 7 35.625576768154296 37.0 35.0 37.0 33.0 37.0 8 35.61541229564894 37.0 35.0 37.0 33.0 37.0 9 37.273140804822 39.0 37.0 39.0 34.0 39.0 10-11 37.177379740863636 39.0 37.0 39.0 34.0 39.0 12-13 37.07470752288786 39.0 37.0 39.0 33.0 39.0 14-15 38.33548331195382 40.0 38.0 41.0 33.5 41.0 16-17 38.26786588296399 40.0 38.0 41.0 33.0 41.0 18-19 38.21272859017465 40.0 38.0 41.0 33.0 41.0 20-21 38.11541450478327 40.0 38.0 41.0 33.0 41.0 22-23 38.026782317303365 40.0 38.0 41.0 33.0 41.0 24-25 38.06131403237217 40.0 38.0 41.0 33.0 41.0 26-27 37.95726773284399 40.0 38.0 41.0 33.0 41.0 28-29 37.821842889312066 40.0 38.0 41.0 33.0 41.0 30-31 37.63805788619227 40.0 37.5 41.0 32.5 41.0 32-33 37.4105293233127 40.0 37.0 41.0 32.0 41.0 34-35 37.07972839429808 40.0 36.5 41.0 31.0 41.0 36-37 36.80590005778114 40.0 36.0 41.0 30.0 41.0 38-39 36.549399434559795 39.5 35.5 41.0 30.0 41.0 40-41 36.386774992795765 39.0 35.5 41.0 30.0 41.0 42-43 36.17515391529786 39.0 35.0 41.0 29.5 41.0 44-45 35.8828697342215 39.0 35.0 41.0 28.5 41.0 46-47 35.479532861364056 39.0 35.0 41.0 26.5 41.0 48-49 35.40045689807103 39.0 35.0 41.0 26.0 41.0 50-51 34.393030033917576 38.0 34.0 40.0 24.0 40.5 52-53 34.555195466659995 38.5 34.5 39.5 24.0 40.5 54-55 35.05079928934603 39.0 35.0 40.5 24.5 41.0 56-57 34.9257600772117 39.0 35.0 41.0 24.0 41.0 58-59 34.728713886176564 39.0 35.0 41.0 24.0 41.0 60-61 34.42521785746371 38.0 34.0 40.0 23.0 41.0 62-63 34.05688275436832 38.0 34.0 40.0 22.0 41.0 64-65 33.675693925911524 37.0 34.0 40.0 21.5 41.0 66-67 33.27597168773444 36.5 33.5 39.5 20.0 41.0 68-69 32.86809946210033 36.0 33.0 39.0 18.5 41.0 70-71 32.44902471628579 35.5 33.0 39.0 17.0 40.5 72-73 31.994496310009293 35.0 32.0 38.0 12.5 40.0 74-75 31.53719494923164 35.0 32.0 37.0 8.0 39.5 76-77 31.09948860994886 35.0 31.0 37.0 7.0 39.0 78-79 30.670884149559377 35.0 31.0 36.0 2.0 39.0 80-81 30.246229130430727 35.0 31.0 36.0 2.0 37.5 82-83 29.819353440383075 34.0 30.0 35.0 2.0 37.0 84-85 29.438958672987738 34.0 30.0 35.0 2.0 37.0 86-87 29.048859669405502 34.0 29.5 35.0 2.0 36.0 88-89 28.634290330324493 34.0 29.0 35.0 2.0 36.0 90-91 28.238030296559103 34.0 29.0 35.0 2.0 35.5 92-93 27.816450637875263 34.0 28.0 35.0 2.0 35.0 94-95 27.34925996454585 34.0 27.0 35.0 2.0 35.0 96-97 26.764419387951968 34.0 25.5 35.0 2.0 35.0 98-99 26.042293140343357 33.0 24.5 35.0 2.0 35.0 100-101 23.484279800117527 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19673136666499857 1101 2 0.20026484405101996 1101 3 0.13241931735534251 1101 4 0.03801608939058809 1101 5 0.24056005794857072 1101 6 0.3384340458721766 1101 7 0.16379098592400965 1101 8 0.34653860959082294 1101 9 0.30215981970749084 1101 10-11 0.35825465857951144 1101 12-13 0.34982742358239705 1101 14-15 0.43400428684661563 1101 16-17 0.607240907512832 1101 18-19 0.4987806447455654 1101 20-21 0.39779439596250654 1101 22-23 0.5425164153158448 1101 24-25 0.5596867621415029 1101 26-27 0.48067172996601926 1101 28-29 0.4322959528448038 1101 30-31 0.5121480704035051 1101 32-33 0.5277291608325854 1101 34-35 0.45722894166175365 1101 36-37 0.4495989177593174 1101 38-39 0.6087308593660836 1101 40-41 0.3538757867885991 1101 42-43 0.4409274726850825 1101 44-45 0.35302949973687703 1101 46-47 0.2716351305661817 1101 48-49 0.4560383908231316 1101 50-51 0.19559194413422887 1101 52-53 0.3989928441502997 1101 54-55 0.3283902930864784 1101 56-57 0.33744909105598 1101 58-59 0.4622505332315683 1101 60-61 0.32075874157282414 1101 62-63 0.34177754240194247 1101 64-65 0.3311921971308749 1101 66-67 0.25093632402636246 1101 68-69 0.27591701927370593 1101 70-71 0.2844449027817788 1101 72-73 0.15134670324472665 1101 74-75 0.18001494559883824 1101 76-77 0.16709161098651748 1101 78-79 0.11207906532333212 1101 80-81 0.054175511389111364 1101 82-83 0.15062197914987152 1101 84-85 0.027002506060266995 1101 86-87 0.06286407931103 1101 88-89 0.028101482911750963 1101 90-91 -0.18949721519058826 1101 92-93 -0.3023245355738169 1101 94-95 -0.41148343602226944 1101 96-97 -0.4135470652521356 1101 98-99 -0.553255684302794 1101 100-101 -0.7259991054625203 1102 1 0.24368193744104616 1102 2 0.32736622181560904 1102 3 0.32989496325267 1102 4 0.27843361257031063 1102 5 0.3363572113816957 1102 6 0.32238895429127723 1102 7 0.43078666284698386 1102 8 0.38226059015852343 1102 9 0.40451077733307983 1102 10-11 0.4245275264917012 1102 12-13 0.5243175669303568 1102 14-15 0.5649884226166009 1102 16-17 0.5717779292083307 1102 18-19 0.5449291733638759 1102 20-21 0.5606650939000204 1102 22-23 0.5284419865319023 1102 24-25 0.5697127443017607 1102 26-27 0.46550894545043775 1102 28-29 0.5426582282775527 1102 30-31 0.6003460033621266 1102 32-33 0.507786896943422 1102 34-35 0.44562148288211034 1102 36-37 0.5950800489816643 1102 38-39 0.5078148076590949 1102 40-41 0.6827505486491461 1102 42-43 0.6522463116023616 1102 44-45 0.4878397984073004 1102 46-47 0.6463256782429241 1102 48-49 0.5342320389405586 1102 50-51 0.5292838142853356 1102 52-53 0.5891505760734006 1102 54-55 0.666643607314036 1102 56-57 0.5342191947600625 1102 58-59 0.4428623854907272 1102 60-61 0.4204181695283111 1102 62-63 0.4809940658662981 1102 64-65 0.521370260807501 1102 66-67 0.47464084234891146 1102 68-69 0.3476374315652464 1102 70-71 0.2519914791876019 1102 72-73 0.2026846843562211 1102 74-75 0.2656597261949436 1102 76-77 0.11491137939874108 1102 78-79 0.13151789071848086 1102 80-81 0.31598188754839995 1102 82-83 0.2777222948079334 1102 84-85 0.3753236797911548 1102 86-87 0.25256601580434435 1102 88-89 0.26702750506790096 1102 90-91 0.0694830472286263 1102 92-93 0.016590398190185596 1102 94-95 -0.08542887045114966 1102 96-97 -0.15148730082129802 1102 98-99 -0.13297360701664118 1102 100-101 -0.197411749628305 1103 1 0.215711016758398 1103 2 0.3028997138393308 1103 3 0.28609676452775545 1103 4 0.34949280130050653 1103 5 0.3362406296282998 1103 6 0.34293866335890755 1103 7 0.33225072086641916 1103 8 0.4262675920238266 1103 9 0.4438005682515396 1103 10-11 0.45085760249604334 1103 12-13 0.5042037344183186 1103 14-15 0.4935777486000674 1103 16-17 0.4753187084616499 1103 18-19 0.532096480360174 1103 20-21 0.5950775914639834 1103 22-23 0.541031945884896 1103 24-25 0.5530833141951277 1103 26-27 0.5383420908609438 1103 28-29 0.5013004162560577 1103 30-31 0.5006955746196766 1103 32-33 0.5704904800053114 1103 34-35 0.530371197517816 1103 36-37 0.29959315485878335 1103 38-39 0.5074755025559554 1103 40-41 0.4581366604562689 1103 42-43 0.49385433938709866 1103 44-45 0.36211681753366776 1103 46-47 0.28511016556161195 1103 48-49 0.19059949070704363 1103 50-51 0.20624360576059786 1103 52-53 0.3250476639655986 1103 54-55 0.3850212417136589 1103 56-57 0.35336783475560907 1103 58-59 0.2594236065714952 1103 60-61 0.37165306610116033 1103 62-63 0.2836846683329668 1103 64-65 0.2675891839401032 1103 66-67 0.2202133457593618 1103 68-69 0.09758436076706545 1103 70-71 0.28311271725102216 1103 72-73 0.047496100319918355 1103 74-75 0.14669761700482198 1103 76-77 -0.0024438156233905772 1103 78-79 0.13089583136271088 1103 80-81 0.13003051083969197 1103 82-83 0.16133455433631738 1103 84-85 0.17754708523117202 1103 86-87 0.1309808171629534 1103 88-89 0.13899028938817537 1103 90-91 0.10932007637754282 1103 92-93 -0.04583739072376147 1103 94-95 0.004076335707150491 1103 96-97 0.18135969519752493 1103 98-99 -0.041057594599813285 1103 100-101 -0.1810607237088746 1104 1 0.3645910110331343 1104 2 0.3748719667326341 1104 3 0.31118826659294285 1104 4 0.31895973787270293 1104 5 0.4240851681307376 1104 6 0.33887474482130386 1104 7 0.3706074664600081 1104 8 0.4371225995893653 1104 9 0.4205212160248166 1104 10-11 0.43411119101278217 1104 12-13 0.45898050005035174 1104 14-15 0.5459639588810106 1104 16-17 0.6039187268643005 1104 18-19 0.5820213362060827 1104 20-21 0.5912053671970199 1104 22-23 0.6742096234704675 1104 24-25 0.5523446522722537 1104 26-27 0.63124352422291 1104 28-29 0.5104953514378323 1104 30-31 0.6447589308540884 1104 32-33 0.6302990924455045 1104 34-35 0.5275443460067564 1104 36-37 0.6657356015933331 1104 38-39 0.638556044820703 1104 40-41 0.5673755575241088 1104 42-43 0.5481459907182185 1104 44-45 0.5920080885433592 1104 46-47 0.6137610468440826 1104 48-49 0.7313536926454489 1104 50-51 0.6865668278601404 1104 52-53 0.7101921247679499 1104 54-55 0.8171985307988407 1104 56-57 0.7674888015081507 1104 58-59 0.8758944894897525 1104 60-61 0.8361679006817795 1104 62-63 0.8316734120453901 1104 64-65 0.7608696607732099 1104 66-67 0.620188553300359 1104 68-69 0.4841716404464478 1104 70-71 0.554908355822981 1104 72-73 0.51612597191032 1104 74-75 0.5428760000253128 1104 76-77 0.574350187157453 1104 78-79 0.5565504033988624 1104 80-81 0.6017441042638119 1104 82-83 0.5768497774173262 1104 84-85 0.6865362685624596 1104 86-87 0.3350010294068504 1104 88-89 0.5307482025276684 1104 90-91 0.5076262658021555 1104 92-93 0.5860086506617534 1104 94-95 0.6225561745817281 1104 96-97 0.5104565851496794 1104 98-99 0.3946110861346561 1104 100-101 0.3794047610987157 1105 1 0.20871422794753158 1105 2 0.24619506169243977 1105 3 0.2214704812642907 1105 4 0.14589470465186594 1105 5 0.13291610116839792 1105 6 0.1697587045291229 1105 7 0.030089559725112736 1105 8 0.17648385690615953 1105 9 0.23826352607189705 1105 10-11 0.26149882649465894 1105 12-13 0.2688104298688785 1105 14-15 0.35264467445399106 1105 16-17 0.27630859553006104 1105 18-19 0.21116242544596986 1105 20-21 0.3147781927078981 1105 22-23 0.35207332878999864 1105 24-25 0.2640975055793575 1105 26-27 0.2944643155075397 1105 28-29 0.43819859379052417 1105 30-31 0.39482041538261115 1105 32-33 0.4624450957866415 1105 34-35 0.18854209801723698 1105 36-37 0.3240521620569581 1105 38-39 0.4703537043781907 1105 40-41 0.4296598098900617 1105 42-43 0.3917521069825156 1105 44-45 0.45718341609755697 1105 46-47 0.4117725124405709 1105 48-49 0.45672348404222873 1105 50-51 0.4608820053674094 1105 52-53 0.4521235313393035 1105 54-55 0.40510646385212823 1105 56-57 0.3661993674427322 1105 58-59 0.3619951475455636 1105 60-61 0.3754299876353073 1105 62-63 0.37533428175603945 1105 64-65 0.5519945849971251 1105 66-67 0.41577093448031377 1105 68-69 0.5099352344220875 1105 70-71 0.3003947608191666 1105 72-73 0.35461413521322527 1105 74-75 0.39758141030738514 1105 76-77 0.41089168311373214 1105 78-79 0.3462685177417164 1105 80-81 0.2956742583877734 1105 82-83 0.2974377586931318 1105 84-85 0.37643997433164245 1105 86-87 0.3756118003995361 1105 88-89 0.3588959978994808 1105 90-91 0.2531132191553418 1105 92-93 0.3727724916722224 1105 94-95 0.20567845901505066 1105 96-97 0.3653373325822038 1105 98-99 0.2651435873910799 1105 100-101 0.4412694381068434 1106 1 0.2538859177381809 1106 2 0.1836845793802908 1106 3 0.13997679371266258 1106 4 0.2966733307981073 1106 5 0.3319280384254668 1106 6 0.26780055620860566 1106 7 0.19840404586120997 1106 8 0.24476092118788983 1106 9 0.3744864135715318 1106 10-11 0.3159490659106723 1106 12-13 0.3381168572033957 1106 14-15 0.4102835021960587 1106 16-17 0.48788515624151074 1106 18-19 0.5589557152986728 1106 20-21 0.5435740475823891 1106 22-23 0.5869721168250663 1106 24-25 0.454089151176575 1106 26-27 0.3715777068709514 1106 28-29 0.44963401016892135 1106 30-31 0.4675759165000244 1106 32-33 0.4602262523630358 1106 34-35 0.4584447330608654 1106 36-37 0.42626086384355943 1106 38-39 0.48943688466977164 1106 40-41 0.45962847825905584 1106 42-43 0.441757977077323 1106 44-45 0.33850831221784716 1106 46-47 0.33620329668523397 1106 48-49 0.4864289153474388 1106 50-51 0.3662394364881365 1106 52-53 0.28712682802647294 1106 54-55 0.2649527092975674 1106 56-57 0.35011059064470373 1106 58-59 0.35420941019876295 1106 60-61 0.4777805937225921 1106 62-63 0.4214791232005908 1106 64-65 0.342544885420196 1106 66-67 0.35974716898221004 1106 68-69 0.38619573245770766 1106 70-71 0.19654819775967525 1106 72-73 0.34030931691362554 1106 74-75 0.3644769582597718 1106 76-77 0.25312607180625335 1106 78-79 0.3770001674060701 1106 80-81 0.6100245175105279 1106 82-83 0.5414484275904314 1106 84-85 0.6196332849393222 1106 86-87 0.4318589701752522 1106 88-89 0.5680540271409384 1106 90-91 0.5712689506739785 1106 92-93 0.38329149191121914 1106 94-95 0.26382201008697237 1106 96-97 0.2036327304278771 1106 98-99 0.2336323157608291 1106 100-101 0.10841056042704267 1107 1 0.2162774009573809 1107 2 0.1844331205799179 1107 3 0.2307062143167684 1107 4 0.28294386527642956 1107 5 0.19918128780376776 1107 6 0.12871557827946845 1107 7 0.2584037191879247 1107 8 0.2973415380287179 1107 9 0.25959235780022993 1107 10-11 0.27339234215784813 1107 12-13 0.2670965544589379 1107 14-15 0.3548187175758457 1107 16-17 0.34878818327781147 1107 18-19 0.46240117413172044 1107 20-21 0.39583350375304605 1107 22-23 0.3126909766944763 1107 24-25 0.45344933092854944 1107 26-27 0.4605734621599993 1107 28-29 0.38710236369474416 1107 30-31 0.2751527204511319 1107 32-33 0.267125336861163 1107 34-35 0.30037735771734475 1107 36-37 0.27899820729560076 1107 38-39 0.35509185949550925 1107 40-41 0.2840714492708898 1107 42-43 0.4394477844625868 1107 44-45 0.3173951372802222 1107 46-47 0.34054766851293294 1107 48-49 0.4114756449395429 1107 50-51 0.36671962069103614 1107 52-53 0.4674580083787063 1107 54-55 0.4146619700767644 1107 56-57 0.3512707912220989 1107 58-59 0.31289902158005845 1107 60-61 0.26645947592829344 1107 62-63 0.24156470649614903 1107 64-65 0.15666829850263753 1107 66-67 0.041634768872775396 1107 68-69 0.1561707827858072 1107 70-71 0.18371899786421153 1107 72-73 0.2341773786793766 1107 74-75 0.28268026541840996 1107 76-77 0.15702338614353195 1107 78-79 0.21491802847192432 1107 80-81 0.25302281166348806 1107 82-83 0.29393191017262055 1107 84-85 0.30560276957138655 1107 86-87 0.30798178197469284 1107 88-89 0.45464403788032115 1107 90-91 0.37749682366340664 1107 92-93 0.3634369023557795 1107 94-95 0.28176530573037795 1107 96-97 0.4080865633263251 1107 98-99 0.2674775818566353 1107 100-101 0.45095186928438125 1108 1 0.03344500169535536 1108 2 0.11748780726405528 1108 3 0.18533620770284642 1108 4 0.127873523988157 1108 5 0.18617821954114078 1108 6 0.19412974400542993 1108 7 0.22999866873813346 1108 8 0.28143282008509374 1108 9 0.33259637090872474 1108 10-11 0.3341909358293691 1108 12-13 0.39157415415260033 1108 14-15 0.45549701745002835 1108 16-17 0.37442222458249574 1108 18-19 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-1.2922579405005266 2316 62-63 -1.4828072554682805 2316 64-65 -1.6119054974613576 2316 66-67 -1.7424743314410236 2316 68-69 -1.6867879876942666 2316 70-71 -1.4956719985040294 2316 72-73 -1.551906204061197 2316 74-75 -1.6305783085761902 2316 76-77 -1.644453112823367 2316 78-79 -1.5677102353577617 2316 80-81 -1.500620500107928 2316 82-83 -1.6104578599349146 2316 84-85 -1.519648492261016 2316 86-87 -1.5115497337362385 2316 88-89 -1.6059491520555014 2316 90-91 -1.574266640577818 2316 92-93 -1.7747874072797103 2316 94-95 -1.575647024736547 2316 96-97 -1.5387354756391751 2316 98-99 -1.5586735121098023 2316 100-101 -1.258867215710282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7546.0 3 2069.0 4 2169.0 5 2393.0 6 2760.0 7 3241.0 8 4044.0 9 4785.0 10 5779.0 11 7079.0 12 9147.0 13 13278.0 14 11867.0 15 12335.0 16 15137.0 17 20120.0 18 19207.0 19 12667.0 20 12405.0 21 13059.0 22 13702.0 23 15062.0 24 16503.0 25 18723.0 26 21245.0 27 24622.0 28 28648.0 29 33783.0 30 40824.0 31 49672.0 32 62038.0 33 80908.0 34 112347.0 35 169636.0 36 265946.0 37 421046.0 38 407332.0 39 73841.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.34926480004886 18.702578470256075 14.426636640009141 22.52152008968592 2 15.143585809163993 20.438436930027322 40.86325963795999 23.554717622848695 3 20.00635943174005 25.08235562394429 29.375610940384995 25.53567400393066 4 12.813161827985791 16.47081432273786 37.01626502559949 33.69975882367686 5 13.753997740717328 37.50497720562716 33.5333883242867 15.207636729368806 6 31.272613640606973 35.538767223708234 17.905384822500732 15.28323431318406 7 28.409527562692066 33.07424772960229 20.49180730635161 18.024417401354032 8 25.953411485360256 34.59514655470642 19.80237915526622 19.649062804667107 9 26.611145095553606 17.59969929910158 20.67146707153193 35.11768853381289 10-11 25.09820029789901 26.554554506355103 26.773077653471667 21.574167542274218 12-13 26.585311505084412 24.064418508037207 27.363739481026723 21.98653050585166 14-15 23.26335410410478 25.19163806723971 26.556597874900223 24.988409953755287 16-17 21.87559264175991 28.624486459026283 27.173723996230674 22.32619690298313 18-19 21.71412131246703 27.264483250932948 29.63320669643285 21.388188740167173 20-21 22.85057522102149 26.045373905949766 29.47696904470556 21.627081828323185 22-23 22.294777173867637 26.0941768896984 29.399597877429777 22.211448059004187 24-25 22.305008864964982 26.24469564439472 29.65747367913988 21.792821811500417 26-27 22.184783159008205 26.11708907269257 29.879125076127462 21.819002692171765 28-29 21.679022515776378 26.710408158350162 29.901589016425916 21.708980309447544 30-31 21.834596273276663 26.414968228072777 29.686546636382648 22.063888862267916 32-33 21.765936765007964 26.472072636992934 29.571853165238743 22.190137432760363 34-35 22.402427413758204 26.391844788157865 29.370332299172198 21.83539549891174 36-37 22.381004062523896 26.091245224006137 29.61153194396825 21.916218769501718 38-39 22.13788591566419 26.363159533960527 29.687070381113795 21.81188416926149 40-41 22.09703246866113 26.453915309542626 29.65729099509007 21.79176122670618 42-43 21.8635582289501 26.639858734280857 29.09513979831162 22.40144323845742 44-45 22.071540458768748 26.711170732681854 28.915261486052028 22.302027322497366 46-47 22.35933228157519 26.886850161956943 28.470111223916938 22.283706332550928 48-49 22.398859509094546 26.83298907934074 28.425487573923334 22.34266383764138 50-51 22.232795619357702 27.186991268314344 28.530634897143702 22.049578215184255 52-53 22.38685397324293 27.31523266595643 28.19909369217946 22.098819668621182 54-55 21.928212787766174 27.046135815324547 28.445906051259385 22.579745345649897 56-57 22.281034465327913 27.42673038641419 27.978775317367887 22.313459830890007 58-59 22.462587992361545 26.91275684007023 28.143330971585563 22.481324195982662 60-61 22.17972109931857 26.930737134087018 28.739983814415815 22.149557952178597 62-63 22.242408250432792 26.91947838009795 28.6328685642136 22.205244805255656 64-65 22.049156387310926 27.146182454000684 28.464490709356955 22.340170449331435 66-67 21.92793792941815 27.2245124523554 28.18083361087469 22.666716007351763 68-69 21.885827193246076 27.21236830950086 28.375888320238925 22.52591617701414 70-71 22.130772409624434 27.18975630516441 28.02040350585028 22.65906777936088 72-73 22.08843356504655 26.93889392422588 28.19798035017987 22.774692160547705 74-75 21.871697900941285 27.617766591262953 27.881823495118663 22.6287120126771 76-77 22.16589438913612 27.06116211119119 27.91601981332145 22.856923686351234 78-79 22.0617737061881 27.141930970229765 28.126594446339183 22.669700877242953 80-81 22.097545637819742 27.362223414158976 27.95699163383703 22.583239314184254 82-83 22.071094955645503 27.239090066254896 27.98021848847686 22.709596489622747 84-85 21.97992950069404 27.22729860195121 27.88345712969366 22.909314767661083 86-87 22.062413349391775 27.165368022598894 28.022766618726468 22.74945200928285 88-89 22.088409486213198 27.014601707368396 27.75619468055111 23.140794125867295 90-91 21.90726151459438 26.99674134339155 28.02560640915187 23.0703907328622 92-93 22.025726288688915 27.00539950596013 28.004210662389887 22.964663542961066 94-95 21.83574900680208 26.89017228095184 28.008489828464118 23.265588883781966 96-97 21.767799188077994 26.67179823084391 28.075996491786416 23.484406089291685 98-99 21.80373855061781 26.76270961907222 27.783431184553592 23.65012064575638 100-101 22.1201257585497 26.102841604511756 28.275989993384503 23.501042643554033 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3402.0 1 2045.0 2 1041.0 3 1300.0 4 943.0 5 785.0 6 1389.5 7 2853.0 8 4420.5 9 4890.5 10 4024.0 11 3197.0 12 3173.5 13 3336.5 14 3552.0 15 3752.0 16 3917.5 17 4079.5 18 4152.5 19 4335.0 20 4674.5 21 5151.5 22 5927.0 23 6993.0 24 8345.5 25 10159.5 26 12500.5 27 15145.5 28 17343.0 29 19717.0 30 22908.5 31 26457.5 32 30521.5 33 33607.5 34 36806.0 35 40386.0 36 44059.5 37 47993.0 38 50979.0 39 53023.5 40 55049.0 41 58145.0 42 60656.0 43 63653.0 44 66994.0 45 69911.5 46 72454.5 47 77752.0 48 94319.0 49 94384.5 50 79224.0 51 73581.0 52 69574.0 53 67757.0 54 66047.5 55 61638.5 56 55861.0 57 50132.5 58 45497.0 59 40796.0 60 34743.5 61 29030.0 62 23992.5 63 18297.0 64 13291.0 65 10051.0 66 7538.5 67 6059.0 68 4744.5 69 3419.0 70 2540.0 71 1924.5 72 1526.0 73 1234.5 74 986.5 75 791.5 76 556.0 77 353.0 78 247.0 79 178.5 80 121.5 81 84.5 82 70.0 83 51.5 84 37.0 85 26.0 86 15.5 87 15.5 88 13.0 89 6.5 90 8.0 91 8.0 92 5.5 93 5.0 94 3.0 95 3.5 96 3.0 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3338738348657362 2 0.11585682256773082 3 0.1091803277078955 4 0.11713321129093465 5 0.13367717281861485 6 0.08448711510129862 7 0.09283273367609281 8 0.0976437373250918 9 0.08600896319434932 10-11 0.08559168226560962 12-13 0.08863537845171102 14-15 0.09059905341048612 16-17 0.07947974395642213 18-19 0.09393730084040379 20-21 0.10441841593286588 22-23 0.11261675888575191 24-25 0.1268534023368714 26-27 0.1266570348409939 28-29 0.12115874495642359 30-31 0.11097218110777778 32-33 0.10363294594935583 34-35 0.32832645310719655 36-37 0.4875804922638571 38-39 0.4614881612491329 40-41 0.44008410419848437 42-43 0.4718710925936563 44-45 0.4794557871219251 46-47 0.4867459304063776 48-49 0.498307066726166 50-51 0.4858622766749288 52-53 0.4900105400253412 54-55 0.47619117750296147 56-57 0.45476257451532826 58-59 0.4338739821413581 60-61 0.3942568398480705 62-63 0.46475277086809647 64-65 0.49676067269613067 66-67 0.5042717294134454 68-69 0.5184101891166261 70-71 0.5097945652350003 72-73 0.501228033227344 74-75 0.5081254415200415 76-77 0.4848313473215719 78-79 0.4639918468215712 80-81 0.46561187866256065 82-83 0.47867031713841507 84-85 0.4783021280836447 86-87 0.4820085645683327 88-89 0.49467426805243203 90-91 0.5103100299116788 92-93 0.5025780597615018 94-95 0.5074381552844702 96-97 0.5068490527968377 98-99 0.500516201054788 100-101 0.4878750435076733 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2036997.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.906898324690104 #Duplication Level Percentage of deduplicated Percentage of total 1 79.0068032089041 46.54045723585714 2 12.48751456033529 14.712015010675165 3 3.745958856661624 6.619884524935162 4 1.6148591695449843 3.8050537963631954 5 0.8237937252520712 2.426356660697072 6 0.5062917203738809 1.7894444936817984 7 0.3474996138631089 1.4329087095192259 8 0.22721270438641641 1.0707516540294801 9 0.17366537493659515 0.9207079725518277 >10 0.9603197062028369 10.431813589823971 >50 0.07111813592635916 2.8526257621619555 >100 0.03120966256000098 3.5204370119611577 >500 0.001918486760325657 0.8128518457403731 >1k 0.0015014244211244245 1.598531650696459 >5k 2.5023740352073744E-4 0.93677207513995 >10k+ 8.34124678402458E-5 0.529388006166159 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10774 0.5289158501460728 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8182 0.40166971281744646 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5577 0.2737853811272182 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5306 0.2604814832815168 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3735 0.18335814927562485 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3323 0.16313229720024133 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3071 0.15076114495995824 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2165 0.10628390714370221 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2087 0.10245474097409078 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.9091873969377474E-5 9.818374793875495E-5 14-15 0.0 0.0 0.0 4.9091873969377474E-5 9.818374793875495E-5 16-17 0.0 0.0 0.0 4.9091873969377474E-5 9.818374793875495E-5 18-19 0.0 0.0 0.0 4.9091873969377474E-5 1.472756219081324E-4 20-21 0.0 0.0 0.0 4.9091873969377474E-5 1.472756219081324E-4 22-23 0.0 0.0 0.0 2.209134328621986E-4 1.472756219081324E-4 24-25 0.0 0.0 0.0 6.381943616019071E-4 1.472756219081324E-4 26-27 0.0 0.0 0.0 0.0010063834163722381 2.209134328621986E-4 28-29 0.0 0.0 0.0 0.0029455124381626485 2.4545936984688734E-4 30-31 0.0 0.0 0.0 0.008713807629564502 2.4545936984688734E-4 32-33 0.0 0.0 0.0 0.019391290217904103 2.4545936984688734E-4 34-35 0.0 0.0 0.0 0.03689254328798717 2.4545936984688734E-4 36-37 0.0 0.0 0.0 0.06163484776855341 2.4545936984688734E-4 38-39 0.0 0.0 0.0 0.09811011012780088 2.4545936984688734E-4 40-41 0.0 0.0 0.0 0.13907727895524638 2.4545936984688734E-4 42-43 0.0 0.0 0.0 0.18242540367020668 2.4545936984688734E-4 44-45 0.0 0.0 0.0 0.23500280069140994 2.7000530683157607E-4 46-47 0.0 0.0 0.0 0.3015222899199165 2.945512438162648E-4 48-49 0.0 0.0 0.0 0.37233731812074344 3.4364311778564227E-4 50-51 0.0 0.0 0.0 0.452651623934645 3.4364311778564227E-4 52-53 0.0 0.0 0.0 0.5317631788362968 3.4364311778564227E-4 54-55 0.0 0.0 0.0 0.6258968471725781 3.4364311778564227E-4 56-57 0.0 0.0 0.0 0.7428091450306505 3.4364311778564227E-4 58-59 0.0 0.0 0.0 0.8748662860082759 3.4364311778564227E-4 60-61 0.0 0.0 0.0 1.0229273778999184 3.4364311778564227E-4 62-63 0.0 0.0 0.0 1.1704730051148822 3.4364311778564227E-4 64-65 2.4545936984688737E-5 0.0 0.0 1.325235137803345 3.4364311778564227E-4 66-67 4.9091873969377474E-5 0.0 0.0 1.502653170328675 3.4364311778564227E-4 68-69 4.9091873969377474E-5 0.0 0.0 1.705623523255066 3.4364311778564227E-4 70-71 4.9091873969377474E-5 0.0 0.0 1.9367235199659105 3.4364311778564227E-4 72-73 4.9091873969377474E-5 0.0 0.0 2.17371454155308 3.4364311778564227E-4 74-75 4.9091873969377474E-5 0.0 0.0 2.420033019194432 3.4364311778564227E-4 76-77 4.9091873969377474E-5 0.0 0.0 2.6902101475849007 3.4364311778564227E-4 78-79 4.9091873969377474E-5 0.0 0.0 3.006656367191508 3.6818905477033106E-4 80-81 4.9091873969377474E-5 0.0 0.0 3.3500294796703187 3.927349917550198E-4 82-83 4.9091873969377474E-5 0.0 0.0 3.7089156243234527 3.927349917550198E-4 84-85 4.9091873969377474E-5 0.0 0.0 4.074895544765162 3.927349917550198E-4 86-87 4.9091873969377474E-5 0.0 0.0 4.468440552440676 3.927349917550198E-4 88-89 4.9091873969377474E-5 0.0 0.0 4.896767152823495 3.927349917550198E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2025 0.0 30.88135 2 GTACCTG 2420 0.0 25.892988 1 GTACATG 10875 0.0 23.17869 1 TACATGG 10840 0.0 23.031845 2 GAGTACT 8395 0.0 22.282488 12-13 ACATGGG 10890 0.0 21.925528 3 GTGTAGC 1110 0.0 20.955439 1 GTACTTT 9095 0.0 20.386269 14-15 ACCTGGG 2950 0.0 20.234661 3 CATGGGG 4480 0.0 19.880495 4 AGTACTT 8715 0.0 19.861282 12-13 ACCGCGC 195 1.060015E-5 19.429607 8 GTATCAA 20940 0.0 18.95192 1 ATGGGAG 2425 0.0 17.973122 5 ACTTTTT 10640 0.0 17.805918 16-17 CATGGGA 6320 0.0 17.76555 4 TACTTTT 10205 0.0 16.799803 14-15 TATCAAC 23445 0.0 16.77163 2 TCAACGC 23455 0.0 16.703884 4 CAACGCA 23525 0.0 16.694458 5 >>END_MODULE