FastQCFastQC Report
Fri 27 May 2016
SRR939165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036997
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106070.5207175071931868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87840.4312230209470117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60040.29474761131214233No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59030.2897893320412352No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA39930.19602385275972423No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34440.16907241395053602No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29540.14501739570554106No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23640.11605319006360833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17750.021.6522082
AATGCGC1100.002896562721.5671568
GTACATG108450.021.4679151
GAGTACT92350.021.40058312-13
AATACCG1605.0342118E-520.7583875
TACATGG108650.020.6997032
AGTACTT94400.020.35778612-13
ATAACGC1404.889669E-420.3347453
ACATGGG109050.020.1016563
GTACTTT98650.019.88959514-15
GTATCAA205350.019.0120831
GTACCTG20850.018.7248521
GTGTAGC12300.018.5800421
GTACACG4201.8189894E-1218.1376611
TATAACG1851.534947E-417.95322
CATGGGG44900.017.7532754
TAACGCA1901.8823467E-417.4807454
ACTTTTT116500.016.94333616-17
GGTATCA165300.016.9361631
TACTTTT112650.016.89120714-15
Produced by FastQC (version 0.11.5)