FastQCFastQC Report
Fri 27 May 2016
SRR939164_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939164_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008133
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105190.523819886431825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81900.40784151248946154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54510.2714461641733889No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53180.2648230968765515No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA37860.18853332921674013No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA31970.15920260261646016No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29600.14740059547848672No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20890.10402697430897258No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20850.10382778431508272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18600.026.4819852
GTACCTG21250.023.4638251
GAGTACT84250.022.73145312-13
GTACATG104250.022.3197061
TACATGG103550.021.9086462
GTACTTT90600.021.19155914-15
ACATGGG106350.020.2640253
GTACACG3052.0918378E-1020.2400681
AGTACTT87350.020.19026212-13
GTATCAA210000.019.7633511
CATGGGG45350.019.2172994
ACTTTTT105950.018.59079716-17
GTATAGG5900.018.5116141
GTACCGG1600.001193738117.8073671
TATCAAC232950.017.6476612
ATCAACG232900.017.6116563
TCAACGC233700.017.5513694
CAACGCA234450.017.4750185
TACTTTT101850.017.15400714-15
AACGCAG238650.017.12016
Produced by FastQC (version 0.11.5)