##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939164_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2008133 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01841262506019 33.0 31.0 34.0 30.0 34.0 2 32.117265141302894 34.0 31.0 34.0 30.0 34.0 3 32.17355075585133 34.0 31.0 34.0 30.0 34.0 4 35.61852028725189 37.0 35.0 37.0 33.0 37.0 5 35.65707998424407 37.0 35.0 37.0 35.0 37.0 6 35.74219038280831 37.0 36.0 37.0 35.0 37.0 7 35.67589596904189 37.0 36.0 37.0 35.0 37.0 8 35.66813104510508 37.0 36.0 37.0 33.0 37.0 9 37.324011905585934 39.0 38.0 39.0 34.0 39.0 10-11 37.22418883609801 39.0 37.0 39.0 34.0 39.0 12-13 37.11814904690078 39.0 37.0 39.0 34.0 39.0 14-15 38.36232684787312 40.0 38.0 41.0 33.5 41.0 16-17 38.267883651132664 40.0 38.0 41.0 33.0 41.0 18-19 38.17759505968977 40.0 38.0 41.0 33.0 41.0 20-21 38.16477494269553 40.0 38.0 41.0 33.5 41.0 22-23 38.20557428218151 40.0 38.0 41.0 33.5 41.0 24-25 38.13993943628236 40.0 38.0 41.0 33.0 41.0 26-27 38.005285506487866 40.0 38.0 41.0 33.0 41.0 28-29 37.85362124919017 40.0 38.0 41.0 33.0 41.0 30-31 37.62797857512426 40.0 37.5 41.0 32.5 41.0 32-33 37.40054568098826 40.0 37.0 41.0 32.0 41.0 34-35 37.06740390203238 40.0 36.5 41.0 31.0 41.0 36-37 36.78693916189815 40.0 36.0 41.0 30.0 41.0 38-39 36.58247237608266 40.0 36.0 41.0 30.0 41.0 40-41 36.380989456375644 39.5 35.5 41.0 30.0 41.0 42-43 36.15364843862433 39.0 35.0 41.0 29.5 41.0 44-45 35.84595542227532 39.0 35.0 41.0 28.5 41.0 46-47 35.63857498482422 39.0 35.0 41.0 26.5 41.0 48-49 35.469153686533716 39.0 35.0 41.0 26.0 41.0 50-51 34.33870316358528 38.0 34.0 40.0 24.0 40.5 52-53 34.48624568193441 38.5 34.5 39.5 24.0 40.5 54-55 35.00472403969259 39.0 35.0 40.5 24.5 41.0 56-57 34.909624760909765 39.0 35.0 41.0 24.0 41.0 58-59 34.696725515690446 39.0 35.0 41.0 23.5 41.0 60-61 34.385420188802236 38.0 34.0 40.0 23.0 41.0 62-63 34.00324306208802 37.5 34.0 40.0 22.0 41.0 64-65 33.61817643552494 37.0 34.0 40.0 20.5 41.0 66-67 33.21644034533569 36.5 33.0 39.5 19.5 41.0 68-69 32.798054212544685 36.0 33.0 39.0 18.0 41.0 70-71 32.35034756164059 35.5 32.5 39.0 14.5 40.5 72-73 31.900197098498957 35.0 32.0 38.0 10.0 40.0 74-75 31.455764633119422 35.0 31.5 37.0 7.5 39.5 76-77 31.009020567860794 35.0 31.0 37.0 4.5 39.0 78-79 30.56612809012152 35.0 31.0 36.0 2.0 39.0 80-81 30.108722629427433 35.0 30.5 36.0 2.0 37.5 82-83 29.69316449657468 34.0 30.0 35.0 2.0 37.0 84-85 29.306553649583968 34.0 30.0 35.0 2.0 37.0 86-87 28.886187319266206 34.0 29.0 35.0 2.0 36.0 88-89 28.43500206410631 34.0 29.0 35.0 2.0 36.0 90-91 28.00293556253495 34.0 28.5 35.0 2.0 35.5 92-93 27.570517988599363 34.0 27.0 35.0 2.0 35.0 94-95 27.058228961926325 34.0 26.0 35.0 2.0 35.0 96-97 26.42376301768857 33.0 25.0 35.0 2.0 35.0 98-99 25.66203259445465 33.0 22.0 35.0 2.0 35.0 100-101 22.69783624889387 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13124632557552474 1101 2 0.22157018934215245 1101 3 0.19663140768285103 1101 4 0.18259069268885497 1101 5 0.1264598967477113 1101 6 0.09938355171688329 1101 7 0.13597961056957786 1101 8 0.26253938843833424 1101 9 0.3106836131212063 1101 10-11 0.3355031812063274 1101 12-13 0.40475457410698823 1101 14-15 0.5202965418122716 1101 16-17 0.3974380359617271 1101 18-19 0.4704993157494144 1101 20-21 0.5437276355074587 1101 22-23 0.5150521543518849 1101 24-25 0.533012764577137 1101 26-27 0.4697071342347101 1101 28-29 0.5216026632237245 1101 30-31 0.49944330179179275 1101 32-33 0.39380191528439923 1101 34-35 0.4316423547289787 1101 36-37 0.4891037139114758 1101 38-39 0.4863159336321843 1101 40-41 0.533367383961199 1101 42-43 0.5007897414507951 1101 44-45 0.4407526254303633 1101 46-47 0.5584503377497256 1101 48-49 0.4745023717468868 1101 50-51 0.5795861740916735 1101 52-53 0.468548694871032 1101 54-55 0.42165037875167855 1101 56-57 0.5247287474698226 1101 58-59 0.42408239032635464 1101 60-61 0.34373570238435747 1101 62-63 0.38950250916595763 1101 64-65 0.3656860300850653 1101 66-67 0.5660799255807376 1101 68-69 0.5319871534614364 1101 70-71 0.4710914866330569 1101 72-73 0.42080748443624216 1101 74-75 0.342567331852095 1101 76-77 0.04006922649440625 1101 78-79 0.1393849155398179 1101 80-81 0.052721586751633964 1101 82-83 -0.05114988484929128 1101 84-85 -0.07967911751017098 1101 86-87 -0.0035547997798488495 1101 88-89 0.14863188690489437 1101 90-91 0.16094852864565112 1101 92-93 0.05452765822644423 1101 94-95 -0.012156040648569899 1101 96-97 -0.10124976403958286 1101 98-99 -0.07990110290579722 1101 100-101 -0.505102828123345 1102 1 0.28570675473802964 1102 2 0.2832965833861678 1102 3 0.2114241312620635 1102 4 0.10587516929130203 1102 5 0.12716861014051517 1102 6 0.25454792714175056 1102 7 0.2545613447690016 1102 8 0.30400960960457724 1102 9 0.37529348546698316 1102 10-11 0.34949216659253324 1102 12-13 0.4673237968136519 1102 14-15 0.35712529409215676 1102 16-17 0.37922108258394616 1102 18-19 0.36839961725451786 1102 20-21 0.43794008490039005 1102 22-23 0.34575291934920216 1102 24-25 0.5428772287024088 1102 26-27 0.49198059781240744 1102 28-29 0.3475868293588533 1102 30-31 0.48382411669778236 1102 32-33 0.35313400217202684 1102 34-35 0.4038927295048822 1102 36-37 0.5162234422994558 1102 38-39 0.4731546809955631 1102 40-41 0.40186640844741106 1102 42-43 0.19844386833973005 1102 44-45 0.2740189011287697 1102 46-47 0.3411820115496127 1102 48-49 0.3217093259096515 1102 50-51 0.17868845986716053 1102 52-53 0.2335433064245862 1102 54-55 0.24191811986598566 1102 56-57 0.21043088381166086 1102 58-59 0.15218719854946272 1102 60-61 0.054140631978611964 1102 62-63 0.04947930277494805 1102 64-65 0.16264917730644157 1102 66-67 0.08372386987904434 1102 68-69 0.036307127070593026 1102 70-71 -0.07894161314266057 1102 72-73 -0.09935874858046034 1102 74-75 -0.026931836601452375 1102 76-77 0.006895083537063584 1102 78-79 -0.16496521811462728 1102 80-81 -0.07577498425143858 1102 82-83 -0.38075754683558927 1102 84-85 -0.20519305389821696 1102 86-87 -0.4840465896688926 1102 88-89 -0.5174530309431447 1102 90-91 -0.5775265362429387 1102 92-93 -0.46635714025265074 1102 94-95 -0.5898570888134316 1102 96-97 -0.3942461820177634 1102 98-99 -0.6988896322365186 1102 100-101 -0.9692147578037229 1103 1 0.1869679737351717 1103 2 0.28094695444611517 1103 3 0.26200819410127707 1103 4 0.17222340060987307 1103 5 0.24573186992157758 1103 6 0.19847386297699643 1103 7 0.12801521073783562 1103 8 0.25369890149061547 1103 9 0.1838208701128181 1103 10-11 0.28091449078574016 1103 12-13 0.3136938598593346 1103 14-15 0.41018665321796277 1103 16-17 0.4242152703600155 1103 18-19 0.39074827494538056 1103 20-21 0.39753596582646367 1103 22-23 0.5137709357162734 1103 24-25 0.5761996278467336 1103 26-27 0.5458931743899029 1103 28-29 0.4632439771725956 1103 30-31 0.5569970534193729 1103 32-33 0.5933056017461382 1103 34-35 0.46423922112678895 1103 36-37 0.4151480128881957 1103 38-39 0.4323057114665261 1103 40-41 0.3863620033131028 1103 42-43 0.4656288705418419 1103 44-45 0.5371880322639058 1103 46-47 0.5543027940221705 1103 48-49 0.7401337092733158 1103 50-51 0.6167785013865341 1103 52-53 0.6339410909990733 1103 54-55 0.5929802633213299 1103 56-57 0.6193533652983731 1103 58-59 0.5974431043859383 1103 60-61 0.5617227895812036 1103 62-63 0.3470677643345539 1103 64-65 0.35540274206585565 1103 66-67 0.3842758631694849 1103 68-69 0.38543501859855667 1103 70-71 0.2569088743450223 1103 72-73 0.3338277062194983 1103 74-75 0.3135763710650643 1103 76-77 0.3015760587201193 1103 78-79 0.4366917391143659 1103 80-81 0.24293310772036136 1103 82-83 0.2184815191281153 1103 84-85 0.1950136877928692 1103 86-87 0.00471960010831296 1103 88-89 0.14844961690205238 1103 90-91 0.329918149893917 1103 92-93 0.28529856473682 1103 94-95 0.24661783860248931 1103 96-97 0.17597146942839004 1103 98-99 -0.027156740424519654 1103 100-101 -0.09590902260778833 1104 1 0.11170682475108151 1104 2 0.13778230736333796 1104 3 0.23447653120074108 1104 4 0.28100888817925096 1104 5 0.31721299599932706 1104 6 0.18442110695336567 1104 7 0.270664346035268 1104 8 0.159013792280696 1104 9 0.35979313962666737 1104 10-11 0.30008694555046844 1104 12-13 0.2845483950534273 1104 14-15 0.39077727733247514 1104 16-17 0.2785927154693155 1104 18-19 0.33404614551263734 1104 20-21 0.34935079351694753 1104 22-23 0.3400491192099011 1104 24-25 0.3170427455858089 1104 26-27 0.40574956923621386 1104 28-29 0.37827412211117206 1104 30-31 0.3162664434486828 1104 32-33 0.3219594849904581 1104 34-35 0.3146348436816808 1104 36-37 0.24299148303256146 1104 38-39 0.07748091404003077 1104 40-41 0.2741179436042174 1104 42-43 0.36152674206154956 1104 44-45 0.36344895266078936 1104 46-47 0.5250623821842098 1104 48-49 0.627378602373085 1104 50-51 0.5325366727840901 1104 52-53 0.5194796162781969 1104 54-55 0.47051585506456917 1104 56-57 0.49569482697072687 1104 58-59 0.4624159239377619 1104 60-61 0.4397000784892455 1104 62-63 0.4448521946880746 1104 64-65 0.3758868945430507 1104 66-67 0.43518741420515283 1104 68-69 0.47433788332016746 1104 70-71 0.4224839973892358 1104 72-73 0.4347516390624726 1104 74-75 0.3860742340127423 1104 76-77 0.34387362212330785 1104 78-79 0.39396736052632164 1104 80-81 0.3024625157032972 1104 82-83 0.42593959361420985 1104 84-85 0.22465085321619682 1104 86-87 0.3574719330074885 1104 88-89 0.3902347639717334 1104 90-91 0.4946018423393461 1104 92-93 0.45871411009590446 1104 94-95 0.36241392928494065 1104 96-97 0.46418154821954616 1104 98-99 0.2624373625016361 1104 100-101 0.1813202384602377 1105 1 0.027595409742744437 1105 2 0.19680066112532302 1105 3 0.2425746352779683 1105 4 0.23264935098793416 1105 5 0.2148007972621926 1105 6 0.20009772072329213 1105 7 0.22492257483843758 1105 8 0.2062540911478905 1105 9 0.3000607438337184 1105 10-11 0.26953290365923266 1105 12-13 0.17780566093322392 1105 14-15 0.22568642254295668 1105 16-17 0.21195626387106614 1105 18-19 0.2561069878318918 1105 20-21 0.3363689388589961 1105 22-23 0.3608147015107406 1105 24-25 0.41890696873253574 1105 26-27 0.4483862909653382 1105 28-29 0.37097927893261584 1105 30-31 0.5281653295081767 1105 32-33 0.522626545209846 1105 34-35 0.4999053180291497 1105 36-37 0.5497495284494747 1105 38-39 0.5702632095236027 1105 40-41 0.5851957541004325 1105 42-43 0.45192816001570435 1105 44-45 0.4840267241510432 1105 46-47 0.6282501883373683 1105 48-49 0.7913039356412099 1105 50-51 0.7119080771036153 1105 52-53 0.773385161296325 1105 54-55 0.8915139303821746 1105 56-57 0.7976074000486904 1105 58-59 0.7675476534855434 1105 60-61 0.6861700386565914 1105 62-63 0.6120581042664313 1105 64-65 0.6817259710680972 1105 66-67 0.7323975853307232 1105 68-69 0.697795634038286 1105 70-71 0.4976164721255927 1105 72-73 0.6783661588291885 1105 74-75 0.6343841695888592 1105 76-77 0.6364920052270868 1105 78-79 0.7233107329471231 1105 80-81 0.7746551923624772 1105 82-83 0.7119466111948363 1105 84-85 0.6331678930011151 1105 86-87 0.4704270947774525 1105 88-89 0.433703709983984 1105 90-91 0.3639235635386804 1105 92-93 0.3017287821605308 1105 94-95 0.4113728193265409 1105 96-97 0.45519693170639997 1105 98-99 0.25246246022031116 1105 100-101 0.1661961121647444 1106 1 0.23865187939375687 1106 2 0.24097740213387908 1106 3 0.314290842400311 1106 4 0.3728776081693468 1106 5 0.3333443952463 1106 6 0.2875107227807163 1106 7 0.34781989996023555 1106 8 0.38713264411946824 1106 9 0.4144690670388087 1106 10-11 0.38077948360314195 1106 12-13 0.3792306512021639 1106 14-15 0.45351833111298134 1106 16-17 0.515437656836788 1106 18-19 0.508586280250519 1106 20-21 0.4631293244245711 1106 22-23 0.5658319564100296 1106 24-25 0.5245954965225081 1106 26-27 0.5464040202183114 1106 28-29 0.46781084024851083 1106 30-31 0.32997641304323366 1106 32-33 0.2605082552435647 1106 34-35 0.3815962017001411 1106 36-37 0.5719814533608769 1106 38-39 0.44972871617987664 1106 40-41 0.5834487026398634 1106 42-43 0.6173561734383171 1106 44-45 0.5085209076442112 1106 46-47 0.4586043421664101 1106 48-49 0.5267849393645463 1106 50-51 0.6773460769197754 1106 52-53 0.6224408229573086 1106 54-55 0.6641241750601665 1106 56-57 0.5880290301231739 1106 58-59 0.6626384615776928 1106 60-61 0.7039798636790877 1106 62-63 0.6248405461439219 1106 64-65 0.5224000129133586 1106 66-67 0.5144330563196817 1106 68-69 0.7200396163850655 1106 70-71 0.7180956147483144 1106 72-73 0.7576759969020834 1106 74-75 0.602953272595105 1106 76-77 0.5614686563581053 1106 78-79 0.6397029156436567 1106 80-81 0.557368433072007 1106 82-83 0.46929086601128844 1106 84-85 0.6107009190258808 1106 86-87 0.6106265559661281 1106 88-89 0.6274431490891104 1106 90-91 0.6318202020959198 1106 92-93 0.6185072567162209 1106 94-95 0.6958288559312145 1106 96-97 0.7491782004096059 1106 98-99 0.8179760388784807 1106 100-101 0.5508273078424537 1107 1 -0.07362729834041915 1107 2 0.007542859169177518 1107 3 0.0029828144126895495 1107 4 0.029588566902916114 1107 5 -0.01639125151298515 1107 6 0.020147358537315085 1107 7 0.030482535868870286 1107 8 0.10892330543774165 1107 9 0.06254103457828109 1107 10-11 0.06186902768607894 1107 12-13 0.07139931727318327 1107 14-15 -0.09749292056297776 1107 16-17 -0.057527473213554 1107 18-19 0.030364161833048797 1107 20-21 0.053517172878940755 1107 22-23 0.14974909965434335 1107 24-25 0.06799523123282825 1107 26-27 0.0027550255758868047 1107 28-29 0.13407423831414178 1107 30-31 0.09611537394054182 1107 32-33 -0.0351973404078052 1107 34-35 0.0030393588677526395 1107 36-37 0.10334275962613759 1107 38-39 0.10269981194306865 1107 40-41 0.21957602406234145 1107 42-43 0.26262210762048 1107 44-45 0.2817789250004168 1107 46-47 0.3200739346580903 1107 48-49 0.3744773432438535 1107 50-51 0.2813962616200172 1107 52-53 0.42705078211724157 1107 54-55 0.2465767440624731 1107 56-57 0.2449264518114873 1107 58-59 0.40639801195133884 1107 60-61 0.3586824208381003 1107 62-63 0.39923758499100614 1107 64-65 0.48771501136546647 1107 66-67 0.4372496494845066 1107 68-69 0.24790784078400208 1107 70-71 0.20917073205197312 1107 72-73 0.3311762783867813 1107 74-75 0.36691967841561635 1107 76-77 0.3714675877077873 1107 78-79 0.34276203734529886 1107 80-81 0.2395081739738849 1107 82-83 0.2943285717402446 1107 84-85 0.3378826182239578 1107 86-87 0.37231409252883907 1107 88-89 0.3815048465685287 1107 90-91 0.25577069384032214 1107 92-93 0.3100271584003913 1107 94-95 0.4477456785804321 1107 96-97 0.3864992152528295 1107 98-99 0.25076068821244846 1107 100-101 0.11941248643374891 1108 1 0.06957340085937602 1108 2 0.17751202603137983 1108 3 0.19534912154986017 1108 4 0.21458261047109772 1108 5 0.17864541847624338 1108 6 0.08825768818322643 1108 7 0.04262871411964397 1108 8 0.11032797635385094 1108 9 0.2961593855435183 1108 10-11 0.23268322926512042 1108 12-13 0.26802029448939635 1108 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-1.449913399762437 2316 82-83 -1.44326392435147 2316 84-85 -1.4426801748974611 2316 86-87 -1.5483112331616198 2316 88-89 -1.551814590638351 2316 90-91 -1.854640978905902 2316 92-93 -1.8666794596298324 2316 94-95 -1.9354851708202965 2316 96-97 -1.8817342396716121 2316 98-99 -2.2069568730173614 2316 100-101 -2.124870994545926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7097.0 3 2130.0 4 2069.0 5 2255.0 6 2735.0 7 3264.0 8 3867.0 9 4772.0 10 5729.0 11 6970.0 12 9235.0 13 13713.0 14 12031.0 15 12432.0 16 15069.0 17 21163.0 18 18062.0 19 12708.0 20 12499.0 21 13042.0 22 13802.0 23 14773.0 24 16698.0 25 18566.0 26 21381.0 27 24766.0 28 29304.0 29 34077.0 30 40591.0 31 49705.0 32 61783.0 33 80578.0 34 111976.0 35 169247.0 36 265430.0 37 415996.0 38 391698.0 39 66894.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41493230789074 18.72377590039929 14.443182971413886 22.418108820296084 2 15.12247336825569 20.406145629911112 40.911005826652236 23.560375175180965 3 20.013481187660727 25.009298230394695 29.431117811819774 25.54610277012481 4 12.807819629551881 16.451269173983565 37.103649582626325 33.63726161383823 5 13.775885195139342 37.55102244339288 33.47196944387656 15.201122917591212 6 31.226864541078207 35.63084927192449 17.874677324509385 15.267608862487917 7 28.41045750200724 33.14720334037219 20.444080513017056 17.998258644603517 8 26.026306989298487 34.54510477992204 19.756854962699457 19.671733268080015 9 26.702000946898906 17.642171887069846 20.650768732401385 35.00505843362986 10-11 25.105347236602004 26.541224278043448 26.766607934850295 21.586820550504253 12-13 26.549026282194887 24.116818725842702 27.355127222110227 21.979027769852188 14-15 23.267413327060773 25.223086138708595 26.5548503356882 24.954650198542428 16-17 21.859771238255234 28.594894162247257 27.197022005790433 22.34831259370707 18-19 21.761841381594408 27.24475324417061 29.63985613084886 21.353549243386123 20-21 22.847603564127077 26.034769078336833 29.467693494640752 21.64993386289534 22-23 22.243162548660923 26.096683829869953 29.42877935231753 22.231374269151594 24-25 22.288625072160755 26.237226674004884 29.660488011095058 21.813660242739306 26-27 22.1367441262232 26.15278275612977 29.87112563045046 21.839347487196566 28-29 21.62065225979428 26.749770008970337 29.9197953796416 21.709782351593788 30-31 21.81397770328315 26.458940132121494 29.666600955659028 22.06048120893633 32-33 21.75318186009535 26.522919112094684 29.560458457006572 22.1634405708034 34-35 22.412223818176777 26.390531272977935 29.36626057687719 21.830984331968097 36-37 22.400876337486057 26.09900150395213 29.623125817310868 21.876996341250944 38-39 22.140493104387243 26.414511620829394 29.673609665070238 21.77138560971312 40-41 22.133977131616433 26.43350246466019 29.662418742276436 21.77010166144694 42-43 21.848281212849294 26.630526460687516 29.130507689804908 22.390684636658282 44-45 22.055693277479193 26.718025374652584 28.94892029571723 22.277361052150997 46-47 22.319587332123405 26.996666809335334 28.416895875148395 22.266849983392866 48-49 22.410524784924313 26.87629427765633 28.39664890191187 22.316532035507482 50-51 22.237858265855962 27.224813349943915 28.500319020345966 22.037009363854157 52-53 22.42843419019237 27.304365760948198 28.17560061815198 22.091599430707458 54-55 21.929974517666558 27.067464233751664 28.407708324520932 22.59485292406084 56-57 22.296395327219496 27.46918746795639 27.969324212368452 22.265092992455664 58-59 22.503226251958395 26.929508834100847 28.150455678378584 22.41680923556218 60-61 22.21384252339849 26.904842935242907 28.731536704789573 22.149777836569033 62-63 22.22461630092318 26.949076194265725 28.615079692790484 22.211227812020613 64-65 22.071647085946815 27.157444207276853 28.45309351008841 22.31781519668792 66-67 21.929529848496834 27.183947814575106 28.21645054591811 22.670071791009953 68-69 21.88043347622291 27.20403829974761 28.350526821842077 22.565001402187413 70-71 22.070716972118376 27.176927135013436 28.046949351276602 22.705406541591582 72-73 22.045577839089415 26.93646735322237 28.221224579919184 22.79673022776903 74-75 21.875066499931776 27.624465966430208 27.85408944851059 22.646378085127424 76-77 22.153495827192927 27.10667689177933 27.922141306785413 22.817685974242327 78-79 22.07212177853512 27.077758051863725 28.156094118883455 22.694026050717703 80-81 22.026498411977087 27.37107385983158 27.971124308211575 22.631303419979762 82-83 22.087980816999412 27.223252655844988 27.993120468633037 22.695646058522566 84-85 21.98325957321572 27.192582478359974 27.900018919695402 22.924139028728906 86-87 22.070003678872013 27.135234834663486 27.98192597709089 22.812835509373617 88-89 22.12696462025372 27.018132260757156 27.69794499376152 23.156958125227607 90-91 21.89054601821663 26.99404595012457 28.037982352140094 23.077425679518708 92-93 22.04945549188489 26.980884697067527 27.996997751067198 22.972662059980387 94-95 21.822501803711162 26.882330940568405 27.995149381330826 23.300017874389606 96-97 21.76448719794216 26.639646011052765 28.057629600886223 23.538237190118853 98-99 21.81011843814855 26.803396678925218 27.68413489581899 23.70234998710724 100-101 22.14868925865608 25.99172516391619 28.270596300275784 23.58898927715195 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3266.0 1 1963.0 2 958.0 3 1203.5 4 957.0 5 832.0 6 1383.0 7 2769.0 8 4399.5 9 4962.0 10 4021.5 11 3152.5 12 3148.0 13 3367.5 14 3593.0 15 3751.0 16 3916.0 17 4052.5 18 4191.5 19 4375.0 20 4650.0 21 5187.0 22 5920.5 23 6887.5 24 8137.0 25 9897.0 26 12214.0 27 14775.0 28 17032.0 29 19565.0 30 22709.0 31 26058.0 32 30080.0 33 33279.0 34 36523.5 35 40002.5 36 43621.0 37 47283.5 38 50174.0 39 52156.0 40 53808.5 41 57185.5 42 59926.5 43 62344.5 44 65839.0 45 69225.0 46 71560.5 47 76924.5 48 93067.5 49 93095.0 50 78263.0 51 72240.5 52 68239.0 53 66734.0 54 65335.5 55 60837.0 56 54930.5 57 49328.0 58 44714.0 59 40113.0 60 34265.0 61 28716.5 62 23549.0 63 17867.0 64 13035.0 65 9913.0 66 7566.5 67 6143.5 68 4765.5 69 3341.0 70 2506.0 71 1882.0 72 1444.5 73 1201.0 74 932.0 75 757.0 76 575.0 77 348.5 78 230.5 79 169.0 80 123.0 81 85.5 82 53.5 83 37.5 84 28.5 85 25.5 86 20.0 87 15.0 88 10.0 89 5.0 90 4.5 91 6.5 92 7.0 93 2.0 94 1.0 95 3.0 96 3.5 97 2.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38971522304548556 2 0.11702412141028506 3 0.11826905887209663 4 0.1302702560039599 5 0.13161478846271635 6 0.07335171524993614 7 0.08216587247956186 8 0.07937721256510401 9 0.07882944008190693 10-11 0.07741021137544177 12-13 0.08079644127156917 14-15 0.07514442519494476 16-17 0.07706162888613453 18-19 0.08597538111270518 20-21 0.08440675991082264 22-23 0.09476463959309468 24-25 0.1093055091470535 26-27 0.11084923159969982 28-29 0.1034045055780668 30-31 0.09329561338815706 32-33 0.09152780219238467 34-35 0.3432292582214425 36-37 0.534277361111042 38-39 0.5149559317037268 40-41 0.4872436238038018 42-43 0.5158024891777586 44-45 0.5253138113859989 46-47 0.5227492402146671 48-49 0.5297706874992841 50-51 0.5284261550405277 52-53 0.5261603688600307 54-55 0.5216038977498004 56-57 0.4919743861586857 58-59 0.48223897520731945 60-61 0.42783520812615494 62-63 0.5053450144985417 64-65 0.5337046898786086 66-67 0.5451332157780385 68-69 0.5603712503106119 70-71 0.5563625516835787 72-73 0.5412241121479503 74-75 0.5453075070226923 76-77 0.5197364915570831 78-79 0.49376709610369435 80-81 0.500514657146713 82-83 0.5140346779819863 84-85 0.5086814468961965 86-87 0.5098267893610633 88-89 0.5204087577864613 90-91 0.5396554909460678 92-93 0.5459548745028343 94-95 0.5409004284078793 96-97 0.5425686446067068 98-99 0.5421951633681633 100-101 0.5173711103796411 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2008133.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.98779022566822 #Duplication Level Percentage of deduplicated Percentage of total 1 79.16352092804144 46.696811660286045 2 12.382047804092583 14.607792768640183 3 3.696836531615187 6.542046534765107 4 1.5993211351627032 3.773616784978205 5 0.8267878775241446 2.4385194940259862 6 0.5066423288705155 1.7931426848914789 7 0.331169459515247 1.3674468224923317 8 0.24426626033111468 1.1526981538896204 9 0.17729479061907816 0.9412405125427757 >10 0.9676757658154351 10.5359363429403 >50 0.06919068135906364 2.772312233265153 >100 0.031106329047219217 3.4188502438234236 >500 0.0022812840034344925 0.8990132668892235 >1k 0.001520856002289651 1.5913900438947077 >5k 2.5347600038160847E-4 0.944915873542116 >10k+ 8.449200012720282E-5 0.5242665791333678 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10519 0.523819886431825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8190 0.40784151248946154 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5451 0.2714461641733889 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5318 0.2648230968765515 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3786 0.18853332921674013 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3197 0.15920260261646016 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2960 0.14740059547848672 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2089 0.10402697430897258 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2085 0.10382778431508272 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.979749847246174E-5 7 0.0 0.0 0.0 0.0 4.979749847246174E-5 8 0.0 0.0 0.0 0.0 4.979749847246174E-5 9 0.0 0.0 0.0 0.0 4.979749847246174E-5 10-11 0.0 0.0 0.0 2.489874923623087E-5 4.979749847246174E-5 12-13 0.0 0.0 0.0 4.979749847246174E-5 9.959499694492347E-5 14-15 0.0 0.0 0.0 7.46962477086926E-5 9.959499694492347E-5 16-17 0.0 0.0 0.0 1.493924954173852E-4 9.959499694492347E-5 18-19 0.0 0.0 0.0 1.493924954173852E-4 9.959499694492347E-5 20-21 0.0 0.0 0.0 1.7429124465361608E-4 1.493924954173852E-4 22-23 0.0 0.0 0.0 5.477724831970791E-4 1.493924954173852E-4 24-25 0.0 0.0 0.0 9.212537217405421E-4 1.493924954173852E-4 26-27 0.0 0.0 0.0 0.0014441274557013904 1.493924954173852E-4 28-29 0.0 0.0 0.0 0.003660116137725937 1.493924954173852E-4 30-31 0.0 0.0 0.0 0.00921253721740542 1.493924954173852E-4 32-33 0.0 0.0 0.0 0.01976960689356731 1.493924954173852E-4 34-35 0.0 0.0 0.0 0.037248528857401375 1.9918999388984695E-4 36-37 0.0 0.0 0.0 0.06416407678176694 1.9918999388984695E-4 38-39 0.0 0.0 0.0 0.10417636680438994 1.9918999388984695E-4 40-41 0.0 0.0 0.0 0.14543359428882452 1.9918999388984695E-4 42-43 0.0 0.0 0.0 0.18853332921674013 1.9918999388984695E-4 44-45 0.0 0.0 0.0 0.24171705758532924 1.9918999388984695E-4 46-47 0.0 0.0 0.0 0.31013882048649166 2.2408874312607782E-4 48-49 0.0 0.0 0.0 0.381996610782254 2.489874923623087E-4 50-51 0.0 0.0 0.0 0.4585104671851914 2.489874923623087E-4 52-53 0.0 0.0 0.0 0.5384603509827287 3.236837400710013E-4 54-55 0.0 0.0 0.0 0.6310090018937988 3.4858248930723216E-4 56-57 0.0 0.0 0.0 0.7429786772091291 3.4858248930723216E-4 58-59 0.0 0.0 0.0 0.8764359731153265 3.4858248930723216E-4 60-61 0.0 0.0 0.0 1.0251562023033336 3.4858248930723216E-4 62-63 0.0 0.0 0.0 1.1702910116013232 3.4858248930723216E-4 64-65 0.0 0.0 0.0 1.3208288494835752 3.4858248930723216E-4 66-67 0.0 0.0 0.0 1.5010210977061778 3.4858248930723216E-4 68-69 0.0 0.0 0.0 1.7101207937920448 3.4858248930723216E-4 70-71 0.0 0.0 0.0 1.9387162105298803 3.4858248930723216E-4 72-73 0.0 0.0 0.0 2.1777691019469327 3.4858248930723216E-4 74-75 0.0 0.0 0.0 2.4252377706058312 3.983799877796939E-4 76-77 0.0 0.0 0.0 2.6949161235834476 3.983799877796939E-4 78-79 0.0 0.0 0.0 3.0116780113667767 3.983799877796939E-4 80-81 0.0 0.0 0.0 3.358716778221363 3.983799877796939E-4 82-83 0.0 0.0 0.0 3.7194498571558756 3.983799877796939E-4 84-85 0.0 0.0 0.0 4.08790154835362 3.983799877796939E-4 86-87 0.0 0.0 0.0 4.479334785096405 4.4817748625215563E-4 88-89 0.0 0.0 0.0 4.905700967017623 4.979749847246174E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1860 0.0 26.481985 2 GTACCTG 2125 0.0 23.463825 1 GAGTACT 8425 0.0 22.731453 12-13 GTACATG 10425 0.0 22.319706 1 TACATGG 10355 0.0 21.908646 2 GTACTTT 9060 0.0 21.191559 14-15 ACATGGG 10635 0.0 20.264025 3 GTACACG 305 2.0918378E-10 20.240068 1 AGTACTT 8735 0.0 20.190262 12-13 GTATCAA 21000 0.0 19.763351 1 CATGGGG 4535 0.0 19.217299 4 ACTTTTT 10595 0.0 18.590797 16-17 GTATAGG 590 0.0 18.511614 1 GTACCGG 160 0.0011937381 17.807367 1 TATCAAC 23295 0.0 17.647661 2 ATCAACG 23290 0.0 17.611656 3 TCAACGC 23370 0.0 17.551369 4 CAACGCA 23445 0.0 17.475018 5 TACTTTT 10185 0.0 17.154007 14-15 AACGCAG 23865 0.0 17.1201 6 >>END_MODULE