FastQCFastQC Report
Fri 27 May 2016
SRR939164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939164_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008133
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103050.5131632217587181No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86910.4327900592241649No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58800.292809291018075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57430.28598703372734774No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38480.19162077412203277No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34100.16980946979109451No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29260.145707480530423No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23600.11752209639500968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18800.023.9768852
GAGTACT87000.022.00761412-13
TACATGG104350.021.9170252
TATCACG1302.964022E-421.8995362
GTACATG104900.021.8931451
GTACCTG21900.021.5391081
ACATGGG103600.020.9306873
AGTACTT89050.020.83490212-13
GTACTTT92850.020.33994714-15
GTAAGCG3100.018.3678194-95
GTATCAA215250.018.0406061
CATGGGA63350.017.9010414
GTGTAGC10150.017.838331
ATTCGCG2153.6379788E-1217.67965180-81
ATAACGC1901.8819491E-417.4812093
TACTTTT105750.017.4100514-15
CATGGGG40650.017.3921264
ACTTTTT109050.017.36119716-17
CGCGGAA2257.2759576E-1216.97706682-83
GGTATCA173750.016.6182171
Produced by FastQC (version 0.11.5)