FastQCFastQC Report
Fri 27 May 2016
SRR939163_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939163_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2063770
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53660.26000959409236496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45350.21974347916676762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28420.13770914394530398No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23560.11416000814044201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85550.028.355451
GGTATCA60400.027.7442781
ATCAACG98950.023.7940923
TCAACGC98750.023.7463364
CAACGCA100050.023.532495
TATCAAC101900.023.2912122
AACGCAG103300.022.783276
TACATGG62900.022.0671122
ACATGGG62100.021.5122593
GTACATG66500.021.4872341
ACGCAGA113150.020.6325237
CGCAGAG112800.020.57068
GAGTACT61400.020.1685112-13
AGAGTAC88850.019.90666210-11
GTACTTT62200.019.72044814-15
GTAAGGT8600.019.2796064
CATGGGG40500.018.7151574
ACTAGCG650.007513881718.29129288-89
GCAGAGT125800.018.1813649
AGTACTT65400.017.95744112-13
Produced by FastQC (version 0.11.5)