##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939163_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2063770 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91510827272419 33.0 31.0 34.0 30.0 34.0 2 32.02781220775571 34.0 31.0 34.0 30.0 34.0 3 32.081935002446976 34.0 31.0 34.0 30.0 34.0 4 35.52380061731685 37.0 35.0 37.0 33.0 37.0 5 35.56536581111267 37.0 35.0 37.0 33.0 37.0 6 35.64295875993933 37.0 35.0 37.0 33.0 37.0 7 35.55784898510977 37.0 35.0 37.0 33.0 37.0 8 35.54155404914307 37.0 35.0 37.0 33.0 37.0 9 37.20088333486774 39.0 37.0 39.0 34.0 39.0 10-11 37.10845370365884 39.0 37.0 39.0 33.5 39.0 12-13 37.00864388958072 39.0 37.0 39.0 33.0 39.0 14-15 38.29576333603066 40.0 38.0 41.0 33.0 41.0 16-17 38.21392015583132 40.0 38.0 41.0 33.0 41.0 18-19 38.130524477049285 40.0 38.0 41.0 33.0 41.0 20-21 38.014360369614835 40.0 38.0 41.0 33.0 41.0 22-23 37.93511704308135 40.0 38.0 41.0 33.0 41.0 24-25 37.970045838441294 40.0 38.0 41.0 33.0 41.0 26-27 37.87826308164185 40.0 38.0 41.0 33.0 41.0 28-29 37.75069096847032 40.0 38.0 41.0 32.0 41.0 30-31 37.575392122184155 40.0 37.5 41.0 32.0 41.0 32-33 37.36562843727741 40.0 37.0 41.0 31.5 41.0 34-35 37.05171118874681 40.0 37.0 41.0 30.5 41.0 36-37 36.789122334368656 40.0 36.5 41.0 30.0 41.0 38-39 36.535048236964386 40.0 36.0 41.0 30.0 41.0 40-41 36.378918920228514 40.0 36.0 41.0 30.0 41.0 42-43 36.18652393435315 39.0 35.5 41.0 29.5 41.0 44-45 35.933892584929524 39.0 35.0 41.0 28.0 41.0 46-47 35.588444691026616 39.0 35.0 41.0 26.5 41.0 48-49 35.569354627695915 39.0 35.0 41.0 26.0 41.0 50-51 34.57568890913231 38.0 34.0 40.0 25.0 40.5 52-53 34.736157614462854 38.5 34.5 39.5 25.0 40.5 54-55 35.23959573983535 39.0 35.0 40.5 26.0 41.0 56-57 35.111182447656475 39.0 35.0 41.0 26.0 41.0 58-59 34.907413616827455 39.0 35.0 41.0 25.0 41.0 60-61 34.60271541886935 38.0 34.5 40.0 24.5 41.0 62-63 34.230318300973465 38.0 34.0 40.0 23.0 41.0 64-65 33.855471297673674 37.0 34.0 40.0 22.0 41.0 66-67 33.453679915882105 36.5 33.5 39.5 21.5 41.0 68-69 33.042008799430164 36.0 33.0 39.0 20.0 41.0 70-71 32.629911036598074 35.5 33.0 39.0 19.0 40.5 72-73 32.17810632967821 35.0 32.0 38.0 17.5 40.0 74-75 31.729267554039453 35.0 32.0 37.0 14.0 39.5 76-77 31.295690411237686 35.0 31.0 37.0 9.5 39.0 78-79 30.870760307592416 35.0 31.0 36.0 7.0 39.0 80-81 30.453956594000303 35.0 31.0 36.0 4.5 37.5 82-83 30.02444700717619 34.5 30.0 35.0 2.0 37.0 84-85 29.647401357709434 34.0 30.0 35.0 2.0 37.0 86-87 29.265793668868138 34.0 30.0 35.0 2.0 36.0 88-89 28.860072101057774 34.0 29.0 35.0 2.0 36.0 90-91 28.465947513531063 34.0 29.0 35.0 2.0 35.5 92-93 28.054194750384006 34.0 29.0 35.0 2.0 35.0 94-95 27.585897411048713 34.0 27.0 35.0 2.0 35.0 96-97 27.0116977667085 34.0 26.5 35.0 2.0 35.0 98-99 26.307443901209922 33.5 25.0 35.0 2.0 35.0 100-101 23.76347485427155 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1291713238854264 1101 2 0.23770190247093836 1101 3 0.33313886429252904 1101 4 0.19591303937632176 1101 5 0.20281095859153453 1101 6 0.39278630449638996 1101 7 0.21068804055011725 1101 8 0.28883209572141766 1101 9 0.3369713992361767 1101 10-11 0.3240763846108976 1101 12-13 0.1711589881578206 1101 14-15 0.23739843753874368 1101 16-17 0.2934414761118447 1101 18-19 0.339187243944572 1101 20-21 0.3094537176707419 1101 22-23 0.3963431069620995 1101 24-25 0.4999774635877827 1101 26-27 0.33026289895985883 1101 28-29 0.25241254848168637 1101 30-31 0.2210587315145105 1101 32-33 0.24404689409200842 1101 34-35 0.22488978839088247 1101 36-37 0.054495491343288904 1101 38-39 -0.08229977397247978 1101 40-41 -0.293196792723748 1101 42-43 -0.2265575307264598 1101 44-45 -0.06512873325090851 1101 46-47 -0.3483004436844084 1101 48-49 -0.3585439714864762 1101 50-51 -0.22232756126771136 1101 52-53 -0.0993005502566433 1101 54-55 -0.018980396752908746 1101 56-57 0.01750339143995916 1101 58-59 0.009237770064366657 1101 60-61 -0.20319209853204967 1101 62-63 -0.08014960339337307 1101 64-65 -0.24024341006609973 1101 66-67 -0.353443324225438 1101 68-69 -0.27182192759020296 1101 70-71 -0.3373047354223644 1101 72-73 -0.37279196670651515 1101 74-75 -0.42729511375543083 1101 76-77 -0.3934219435991082 1101 78-79 -0.41877997320504434 1101 80-81 -0.505955918156296 1101 82-83 -0.41677526290957445 1101 84-85 -0.43872825583878594 1101 86-87 -0.567672159618553 1101 88-89 -0.5095056428358262 1101 90-91 -0.7836098558930118 1101 92-93 -0.7079377291265097 1101 94-95 -0.8192740829745304 1101 96-97 -0.8128183737008357 1101 98-99 -0.8572945376405379 1101 100-101 -1.178825172520277 1102 1 0.17446533444431722 1102 2 0.23227408524901705 1102 3 0.31254416801241547 1102 4 0.3908365784260255 1102 5 0.22938933106559745 1102 6 0.26234240620159 1102 7 0.3223152630757582 1102 8 0.3802906186900472 1102 9 0.45576956660070067 1102 10-11 0.32382621773184184 1102 12-13 0.4322130569726781 1102 14-15 0.47959350398694767 1102 16-17 0.37460674706274943 1102 18-19 0.4260488556928621 1102 20-21 0.634184283845876 1102 22-23 0.5258298780040249 1102 24-25 0.5227464422879464 1102 26-27 0.4634103897056576 1102 28-29 0.4806193571472619 1102 30-31 0.5320173758497262 1102 32-33 0.45308385021797903 1102 34-35 0.5075706246066716 1102 36-37 0.46613469881947367 1102 38-39 0.38378721552930983 1102 40-41 0.49377905532527677 1102 42-43 0.41758364895677147 1102 44-45 0.2797585475306974 1102 46-47 0.4443283566068743 1102 48-49 0.3361776439028077 1102 50-51 0.23158211164933107 1102 52-53 0.38454934961591647 1102 54-55 0.3683721428429436 1102 56-57 0.31818122383269554 1102 58-59 0.347963117476219 1102 60-61 0.2164853697609317 1102 62-63 0.29891041238428073 1102 64-65 0.22547978308882222 1102 66-67 0.2382799296127729 1102 68-69 0.054875990972810484 1102 70-71 -0.13825002943292475 1102 72-73 -0.2957214527190182 1102 74-75 -0.07991845255025964 1102 76-77 -0.12283392248131975 1102 78-79 -0.12838856595945813 1102 80-81 -0.24835260484844923 1102 82-83 -0.2552583322705786 1102 84-85 -0.10200833958173305 1102 86-87 -0.2322188554132012 1102 88-89 -0.07695694449247625 1102 90-91 -0.18340596002554577 1102 92-93 -0.4726998506144646 1102 94-95 -0.5355059538404809 1102 96-97 -0.5701822914142909 1102 98-99 -0.5539416339449268 1102 100-101 -0.5357409441083583 1103 1 0.2981492942718411 1103 2 0.38869684649404945 1103 3 0.35733050989262694 1103 4 0.38157392778369115 1103 5 0.39018365631634566 1103 6 0.363677721373719 1103 7 0.28137119933187194 1103 8 0.4251370192798589 1103 9 0.5390630087221524 1103 10-11 0.487969185862859 1103 12-13 0.47876462196064296 1103 14-15 0.48115340323033706 1103 16-17 0.5057615074107531 1103 18-19 0.6616806230224626 1103 20-21 0.5340239596353484 1103 22-23 0.5900426976687285 1103 24-25 0.49106088720642305 1103 26-27 0.5009367282604487 1103 28-29 0.5300651306413116 1103 30-31 0.5234527967606866 1103 32-33 0.5462931923946428 1103 34-35 0.33014048057819423 1103 36-37 0.3291472910303028 1103 38-39 0.37157858163123336 1103 40-41 0.2582628196513497 1103 42-43 0.3890356437047444 1103 44-45 0.25539823195915545 1103 46-47 0.1862896610465441 1103 48-49 0.21753373406306054 1103 50-51 0.15774436602251996 1103 52-53 0.3050828011337501 1103 54-55 0.24510681884839158 1103 56-57 0.28221508037699294 1103 58-59 0.19425823473272175 1103 60-61 0.2722256214631429 1103 62-63 0.289537407561248 1103 64-65 0.21260147978583177 1103 66-67 0.2343136879329819 1103 68-69 0.11885641597546481 1103 70-71 0.11780450979251356 1103 72-73 -0.03253856596015936 1103 74-75 0.02056331889173535 1103 76-77 0.04734006434599891 1103 78-79 0.004463074826738023 1103 80-81 -0.09967386687424451 1103 82-83 0.06490133514026653 1103 84-85 -0.09044999653940167 1103 86-87 -0.15288884434227867 1103 88-89 0.01592592090559819 1103 90-91 -0.09505442071785808 1103 92-93 -0.2348722421877838 1103 94-95 -0.2553406533368765 1103 96-97 -0.22491660410892678 1103 98-99 -0.4151755416130918 1103 100-101 -0.5875220546678648 1104 1 0.07683586740983728 1104 2 0.14469765618643748 1104 3 0.22424997540364444 1104 4 0.12023363385615227 1104 5 0.15347974840045708 1104 6 0.1443821997547019 1104 7 -0.023222079761275438 1104 8 0.07390994427060349 1104 9 0.06395299867000404 1104 10-11 0.16978940655003782 1104 12-13 0.12145622807290124 1104 14-15 0.18324238658333059 1104 16-17 0.22456851078983675 1104 18-19 0.2159193741639669 1104 20-21 0.14918832488942257 1104 22-23 0.08056992932586127 1104 24-25 0.2288309667724988 1104 26-27 0.3048471169357967 1104 28-29 0.3163828244901765 1104 30-31 0.22911782564048622 1104 32-33 0.15785793443170348 1104 34-35 0.22257733262300405 1104 36-37 0.19889924222793098 1104 38-39 0.2607168573084806 1104 40-41 0.2190289680688835 1104 42-43 0.02465796821196875 1104 44-45 0.08627430991965213 1104 46-47 0.11856792149605866 1104 48-49 0.3057677765673006 1104 50-51 0.40661263689222693 1104 52-53 0.29218034146310146 1104 54-55 0.07484656715367066 1104 56-57 0.04555717771030032 1104 58-59 0.1344571614302552 1104 60-61 0.19111434166611474 1104 62-63 0.15527856362714942 1104 64-65 0.06755382984897551 1104 66-67 0.03339496310647405 1104 68-69 0.010315368941995473 1104 70-71 -0.04849900293829279 1104 72-73 -0.046808244130438936 1104 74-75 -0.14899362755585344 1104 76-77 -0.027222368227558746 1104 78-79 -0.11779271205854869 1104 80-81 -0.1002658969248742 1104 82-83 -0.17157887225139845 1104 84-85 -0.18168473142265285 1104 86-87 -0.25728999401345476 1104 88-89 0.09538820005164794 1104 90-91 -0.05459711703950987 1104 92-93 -0.22374098461129321 1104 94-95 -0.4416343094430353 1104 96-97 -0.4317674182868245 1104 98-99 -0.4174378497424449 1104 100-101 -0.32976643225134694 1105 1 0.03360292311471014 1105 2 0.15956299898521564 1105 3 0.20831023114019587 1105 4 0.2780281208266686 1105 5 0.1487620883865972 1105 6 0.2440092273667105 1105 7 0.16929521051567065 1105 8 0.2503773167175041 1105 9 0.17087546883388427 1105 10-11 0.13225396971045456 1105 12-13 0.23441174695971512 1105 14-15 0.282179922912718 1105 16-17 0.34488320554491025 1105 18-19 0.3570451583487326 1105 20-21 0.3178938998392482 1105 22-23 0.38325339481169607 1105 24-25 0.3607078892315059 1105 26-27 0.3488614567925268 1105 28-29 0.26686691877420543 1105 30-31 0.2279769016552322 1105 32-33 0.23469674520428896 1105 34-35 0.1723772351124282 1105 36-37 0.10564178082772457 1105 38-39 0.24491266837002001 1105 40-41 0.02107161388569523 1105 42-43 0.07257147610486214 1105 44-45 0.16123417327096234 1105 46-47 0.20448112927057593 1105 48-49 0.15741781399842836 1105 50-51 0.20348883973655063 1105 52-53 0.08663174962074294 1105 54-55 -0.013776770898992652 1105 56-57 0.019448273789464565 1105 58-59 0.19938286260830296 1105 60-61 0.15006562319388905 1105 62-63 0.19735900873951806 1105 64-65 0.24339251566519948 1105 66-67 0.08570816173240559 1105 68-69 0.029924729734482014 1105 70-71 0.014691673756118462 1105 72-73 0.08631266340967159 1105 74-75 0.2296185313663095 1105 76-77 0.11698618391884708 1105 78-79 0.19456036660928433 1105 80-81 -0.025277165309908867 1105 82-83 0.10547250377461026 1105 84-85 0.08002853168967405 1105 86-87 0.12034413044975878 1105 88-89 -0.10831979592743579 1105 90-91 0.08509919998367366 1105 92-93 -0.1329505743416668 1105 94-95 -0.11344147948296879 1105 96-97 -0.22691891670311293 1105 98-99 -0.20571326246099275 1105 100-101 -0.3148381928974615 1106 1 0.3177583732618139 1106 2 0.28560639589311165 1106 3 0.3522185385336627 1106 4 0.31149735511184673 1106 5 0.3691179806315148 1106 6 0.3548692818868986 1106 7 0.3552922321327827 1106 8 0.4521981757943152 1106 9 0.55319527303309 1106 10-11 0.5022287566138601 1106 12-13 0.43835017216603234 1106 14-15 0.398884427629298 1106 16-17 0.530369689563635 1106 18-19 0.5438397013738125 1106 20-21 0.5046775345168015 1106 22-23 0.5505838097356062 1106 24-25 0.6867996060788144 1106 26-27 0.6331638329538194 1106 28-29 0.6160448737849507 1106 30-31 0.6194178351081163 1106 32-33 0.5274126894106459 1106 34-35 0.44104660256024886 1106 36-37 0.5342894716382958 1106 38-39 0.6934760390009203 1106 40-41 0.6797842995935781 1106 42-43 0.500055744517006 1106 44-45 0.5633294648241574 1106 46-47 0.6343768158406249 1106 48-49 0.6528699757770298 1106 50-51 0.69394201558746 1106 52-53 0.6454094275325204 1106 54-55 0.6618717303613977 1106 56-57 0.8209244290844708 1106 58-59 0.6765568087339986 1106 60-61 0.8283111191910635 1106 62-63 0.8107399689057289 1106 64-65 0.7194896328178899 1106 66-67 0.6967251079176151 1106 68-69 0.6511665683378141 1106 70-71 0.7512494248373258 1106 72-73 0.8076536737058078 1106 74-75 0.5809809839722355 1106 76-77 0.5117788081521297 1106 78-79 0.5048523520982222 1106 80-81 0.7212463712679309 1106 82-83 0.7225058749684372 1106 84-85 0.8581113990390534 1106 86-87 0.7260310694947236 1106 88-89 0.7073557957426821 1106 90-91 0.762404154016437 1106 92-93 0.7027169209105075 1106 94-95 0.7212757774958867 1106 96-97 0.9054115938371794 1106 98-99 0.8911109808969648 1106 100-101 0.6871173640706445 1107 1 0.07840922978337161 1107 2 0.2157701595948609 1107 3 0.17670193034068404 1107 4 0.09902437138427445 1107 5 0.2373934635617161 1107 6 0.10868977257679546 1107 7 0.16182897273478858 1107 8 0.17625975200593302 1107 9 0.1254681962319779 1107 10-11 0.2142978336941752 1107 12-13 0.2976401014779029 1107 14-15 0.3353324998316509 1107 16-17 0.3165621962355445 1107 18-19 0.2827155187402468 1107 20-21 0.30096133861176355 1107 22-23 0.4072636945476944 1107 24-25 0.4566772647806303 1107 26-27 0.2851806148865208 1107 28-29 0.17596788314040168 1107 30-31 0.3215916451438474 1107 32-33 0.31983880926762254 1107 34-35 0.3440343653734317 1107 36-37 0.3417127433865801 1107 38-39 0.45738820923936174 1107 40-41 0.3824106735894759 1107 42-43 0.4781989305061458 1107 44-45 0.5611231995964587 1107 46-47 0.6654205636051813 1107 48-49 0.6057265166955403 1107 50-51 0.48008739344843576 1107 52-53 0.5371225776426272 1107 54-55 0.6321677145790972 1107 56-57 0.6329352447644538 1107 58-59 0.6244608758739503 1107 60-61 0.523087998662568 1107 62-63 0.4799652629640363 1107 64-65 0.4690524812526533 1107 66-67 0.5289782573959414 1107 68-69 0.5817820043735722 1107 70-71 0.49523171255642495 1107 72-73 0.6153536043118137 1107 74-75 0.5926319902869857 1107 76-77 0.5580657966262095 1107 78-79 0.49469583859084665 1107 80-81 0.6367346053692202 1107 82-83 0.6919971028955949 1107 84-85 0.6094543154004448 1107 86-87 0.6491171047949678 1107 88-89 0.7352723558387915 1107 90-91 0.8577652269433038 1107 92-93 0.5808418158259983 1107 94-95 0.53762231640696 1107 96-97 0.6827250325284524 1107 98-99 0.7219814729625114 1107 100-101 0.8945136832340879 1108 1 0.1328094753111877 1108 2 0.18879826431950875 1108 3 0.18688517697689377 1108 4 0.11561313770474158 1108 5 0.20492993597600417 1108 6 0.19519043428989846 1108 7 0.3106044613957408 1108 8 0.30931882138513345 1108 9 0.21654367062260604 1108 10-11 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-1.3850709406915058 2316 66-67 -1.3027658043457961 2316 68-69 -1.3601122779018304 2316 70-71 -1.1312425174984497 2316 72-73 -1.1730896241318334 2316 74-75 -1.2574176153672916 2316 76-77 -1.3562330332187074 2316 78-79 -0.969753538646696 2316 80-81 -1.0214265822439792 2316 82-83 -1.0297104660043495 2316 84-85 -0.9194068502939778 2316 86-87 -0.7409700319589803 2316 88-89 -0.94262621021171 2316 90-91 -1.139676909406873 2316 92-93 -1.4094647009726309 2316 94-95 -1.2659095869504604 2316 96-97 -1.382902190593164 2316 98-99 -1.4812323197166144 2316 100-101 -1.2825442784734236 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8726.0 3 2447.0 4 2410.0 5 2577.0 6 3069.0 7 3519.0 8 4256.0 9 5057.0 10 6176.0 11 7332.0 12 9110.0 13 12586.0 14 11384.0 15 11561.0 16 13603.0 17 16463.0 18 16028.0 19 12288.0 20 12267.0 21 12820.0 22 13668.0 23 14939.0 24 16979.0 25 18813.0 26 21657.0 27 24874.0 28 29480.0 29 34980.0 30 41360.0 31 50436.0 32 62828.0 33 81404.0 34 112464.0 35 167510.0 36 271413.0 37 431026.0 38 416273.0 39 79955.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15091437992677 18.67952774013747 14.708795288097868 23.460762591837895 2 15.373681018799273 19.69596118859915 40.851182534869615 24.079175257731958 3 19.744868208750894 23.459977510058863 29.30610090590414 27.489053375286097 4 13.133551683301661 16.34039501905913 36.64587474293099 33.88017855470821 5 14.200631872437658 36.6836353190587 34.19651708341036 14.919215725093274 6 32.89525438179992 35.22012182080343 17.352844320307526 14.531779477089124 7 28.89008745606219 31.967273942497552 20.641056670616443 18.501581930823814 8 25.947302809967066 35.32132965332583 19.68427281855267 19.047094718154433 9 25.93226331664433 17.485911834931667 20.432750018551417 36.14907482987258 10-11 25.247967734003407 26.224124710637625 27.067125281481147 21.460782273877822 12-13 26.06089427785079 23.46967059877441 27.54252198275622 22.926913140618584 14-15 22.785633130957446 25.099775313052092 25.87338714664552 26.24120440934494 16-17 22.48593097106853 28.04356112777258 26.775311642812525 22.69519625834636 18-19 22.44567585927077 26.851412843582807 28.61471167504294 22.088199622103478 20-21 22.981362090798775 26.0548719955003 28.858113662067925 22.105652251632996 22-23 22.598027788902147 25.987409343806707 28.758246351011664 22.65631651627949 24-25 22.664539545458844 26.1018975483521 28.83639337477435 22.397169531414704 26-27 22.283594252065182 26.351801306004774 28.755952457486433 22.608651984443615 28-29 22.250224756115962 26.846554038744898 28.642856345788193 22.260364859350943 30-31 22.49623059264592 26.36314771225596 28.45516879377024 22.685452901327878 32-33 22.254327128540734 26.32530363027178 28.560503640361663 22.859865600825817 34-35 22.837895093018183 26.500356233651356 28.35926145508138 22.30248721824908 36-37 22.838543270767666 26.146352199772554 28.435879338087624 22.579225191372167 38-39 22.444440820403823 26.473316526980184 28.49404641795633 22.58819623465966 40-41 22.818595469498227 26.355643853871047 28.494425573978255 22.33133510265247 42-43 22.527479348952497 26.43937795025832 28.132967112417212 22.900175588371972 44-45 22.63753984642299 26.54291533398935 28.27648731171089 22.543057507876775 46-47 23.047081471721146 26.776635997085208 27.699961556506214 22.47632097468743 48-49 22.895962638395474 26.803980627676903 27.645712449079475 22.654344284848147 50-51 22.69411495461805 27.00980678586732 27.771415215612947 22.52466304390168 52-53 22.951354515188598 26.938765970024537 27.550370980316153 22.55950853447071 54-55 22.650120807493106 26.91863600124155 27.63588560717177 22.79535758409358 56-57 22.76042309359506 27.068877688235833 27.515808570568375 22.65489064760073 58-59 22.80308577221321 26.775884879467952 27.580713412665215 22.840315935653624 60-61 22.60088878943182 26.878291305965885 27.903196382573505 22.617623522028797 62-63 22.549222212729145 26.948095019371966 27.981306914343477 22.521375853555416 64-65 22.38632766655791 26.982015982546788 27.779925882278462 22.851730468616843 66-67 22.394574447930935 26.985722154499953 27.628729263471225 22.990974134097883 68-69 22.663659457694138 26.699719663245197 27.834704818377777 22.801916060682885 70-71 22.600532869931474 26.728314504791616 27.619889037787694 23.05126358748922 72-73 22.52533989031759 26.793905472091666 27.65107629701814 23.029678340572605 74-75 22.427344832700065 27.236370162623185 27.367577201632443 22.968707803044307 76-77 22.57554738714454 26.885209262646985 27.44928570767747 23.089957642531008 78-79 22.585416743745874 26.983678031549573 27.480133037089544 22.95077218761501 80-81 22.749520718868148 26.887987934480883 27.327154525689373 23.0353368209616 82-83 22.665019020478237 26.923994529269336 27.533487288925762 22.877499161326668 84-85 22.595402787424383 27.059863479550295 27.370526744769467 22.97420698825585 86-87 22.660790025810932 27.025739355851474 27.336605022638732 22.976865595698857 88-89 22.57288899310037 27.034114408138432 27.106551128271917 23.28644547048928 90-91 22.52624181039269 26.920235221691673 27.310355676861427 23.243167291054213 92-93 22.6792018843248 26.781913462388378 27.409177585145137 23.129707068141677 94-95 22.504218292914267 26.5981966721693 27.53748369361009 23.360101341306347 96-97 22.540078114365294 26.358058347373937 27.660565103056268 23.4412984352045 98-99 22.358130301143547 26.55850241758809 27.340088181029465 23.743279100238897 100-101 22.645951608921315 25.893814066028032 27.762761103073903 23.69747322197675 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2020.0 1 1151.5 2 311.5 3 349.5 4 316.5 5 313.5 6 500.0 7 940.5 8 1531.5 9 1873.5 10 1755.5 11 1644.5 12 1778.0 13 1961.0 14 2148.5 15 2277.0 16 2426.0 17 2605.5 18 2813.0 19 3084.5 20 3417.0 21 3985.5 22 4811.5 23 5807.0 24 7198.0 25 9101.5 26 11616.0 27 14736.5 28 17415.5 29 20021.5 30 23346.5 31 27343.5 32 32679.0 33 36398.5 34 38289.0 35 41202.0 36 45145.0 37 49044.5 38 52256.5 39 54818.5 40 57443.0 41 60616.5 42 62486.0 43 64161.0 44 66919.5 45 70390.0 46 72894.5 47 75846.5 48 80549.0 49 80557.0 50 76434.0 51 74044.5 52 74569.0 53 75359.0 54 74285.5 55 69653.0 56 63737.0 57 58193.0 58 53527.0 59 47569.0 60 39257.0 61 32736.0 62 26847.5 63 20136.5 64 14002.0 65 10186.5 66 7638.5 67 5789.5 68 4495.0 69 3596.5 70 2835.5 71 1994.5 72 1428.5 73 1101.5 74 809.5 75 621.5 76 480.0 77 331.5 78 222.5 79 145.5 80 110.5 81 83.5 82 60.5 83 42.5 84 35.5 85 29.5 86 21.0 87 14.0 88 10.0 89 6.5 90 6.5 91 6.5 92 6.0 93 6.0 94 4.5 95 3.5 96 2.0 97 2.5 98 3.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3402026388599505 2 0.12210663009928431 3 0.1164858487137617 4 0.12370564549344161 5 0.14105253976945106 6 0.09153151756251908 7 0.09928431947358474 8 0.10563192603826976 9 0.09366353808806215 10-11 0.0925006178014023 12-13 0.09611051619124225 14-15 0.0975157115376229 16-17 0.08692829142782384 18-19 0.10148902251704406 20-21 0.11142229996559694 22-23 0.12014420211554583 24-25 0.13414770056740818 26-27 0.13332396536435745 28-29 0.1282604166161927 30-31 0.11813331911986318 32-33 0.11139807245962485 34-35 0.3311173241204204 36-37 0.4900982183092108 38-39 0.4645139720026941 40-41 0.4418612539187991 42-43 0.4757555347737393 44-45 0.48198200380856393 46-47 0.48971057821365754 48-49 0.5002979983234566 50-51 0.4889110705165789 52-53 0.49215755631683766 54-55 0.4788566555381656 56-57 0.45671271507968425 58-59 0.4353440548123095 60-61 0.39563517252407004 62-63 0.4671789976596229 64-65 0.4981175227859694 66-67 0.5063064198045325 68-69 0.5186382203443214 70-71 0.5111276934929765 72-73 0.5033022090639945 74-75 0.510061683230205 76-77 0.48740896514630994 78-79 0.4654588447356053 80-81 0.4676877752850366 82-83 0.48120672361745737 84-85 0.4815701362070386 86-87 0.4840171143102187 88-89 0.49683346496944913 90-91 0.511588016106446 92-93 0.5043439918207939 94-95 0.5092864030390984 96-97 0.5087049428957684 98-99 0.5029872514863575 100-101 0.49070390595851276 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2063770.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.47964048291974 #Duplication Level Percentage of deduplicated Percentage of total 1 79.88096053574168 49.11052735167698 2 12.042733122558351 14.80765805613274 3 3.5764432301741653 6.596353319960395 4 1.5296961182204425 3.761806695852428 5 0.8033888657463376 2.4696029317032755 6 0.4644420071953879 1.7132236576522846 7 0.3137683196563936 1.3503254441183443 8 0.2120256299808645 1.0428207603510493 9 0.17497028460953576 0.9681399173689563 >10 0.9093653396897987 10.171439226393668 >50 0.0629563146547877 2.619820118804469 >100 0.025457584575443164 2.915411299577483 >500 0.0026074449478058993 1.0519879611274923 >1k 0.0011061887657358013 1.1602179748600112 >5k 7.901348326684294E-5 0.26066528442048315 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5366 0.26000959409236496 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4535 0.21974347916676762 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2842 0.13770914394530398 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2356 0.11416000814044201 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.691002388832089E-5 2 0.0 0.0 0.0 0.0 9.691002388832089E-5 3 0.0 0.0 0.0 0.0 9.691002388832089E-5 4 0.0 0.0 0.0 0.0 9.691002388832089E-5 5 0.0 0.0 0.0 0.0 9.691002388832089E-5 6 0.0 0.0 0.0 0.0 9.691002388832089E-5 7 0.0 0.0 0.0 0.0 9.691002388832089E-5 8 0.0 0.0 0.0 0.0 9.691002388832089E-5 9 0.0 0.0 0.0 0.0 9.691002388832089E-5 10-11 0.0 0.0 0.0 0.0 1.4536503583248133E-4 12-13 0.0 0.0 0.0 0.0 2.18047553748722E-4 14-15 0.0 0.0 0.0 0.0 2.9073007166496265E-4 16-17 0.0 0.0 0.0 0.0 5.330051313857649E-4 18-19 0.0 0.0 0.0 0.0 5.814601433299253E-4 20-21 0.0 0.0 0.0 0.0 6.056876493020055E-4 22-23 0.0 0.0 0.0 4.8455011944160446E-5 6.783701672182462E-4 24-25 0.0 0.0 0.0 1.9382004777664178E-4 6.783701672182462E-4 26-27 0.0 0.0 0.0 4.8455011944160443E-4 6.783701672182462E-4 28-29 4.8455011944160446E-5 0.0 0.0 0.0016959254180456155 7.025976731903264E-4 30-31 4.8455011944160446E-5 0.0 0.0 0.005620781385522611 7.510526851344869E-4 32-33 4.8455011944160446E-5 0.0 0.0 0.014875688666857257 8.479627090228078E-4 34-35 4.8455011944160446E-5 0.0 0.0 0.026553346545399925 8.72190214994888E-4 36-37 4.8455011944160446E-5 0.0 0.0 0.04787355180083051 8.72190214994888E-4 38-39 4.8455011944160446E-5 0.0 0.0 0.07624396129413646 9.933277448552892E-4 40-41 4.8455011944160446E-5 0.0 0.0 0.10916914191019347 0.0010660102627715298 42-43 4.8455011944160446E-5 0.0 0.0 0.14488048571303974 0.0011144652747156901 44-45 4.8455011944160446E-5 0.0 0.0 0.18865958900458868 0.0011629202866598506 46-47 4.8455011944160446E-5 0.0 0.0 0.2454730905091168 0.0011629202866598506 48-49 4.8455011944160446E-5 0.0 0.0 0.304418612539188 0.0012598303105481716 50-51 4.8455011944160446E-5 0.0 0.0 0.3731035919700354 0.0014051953463806528 52-53 4.8455011944160446E-5 0.0 0.0 0.4387601331543728 0.0015990153941572948 54-55 4.8455011944160446E-5 0.0 0.0 0.5124359788154688 0.0015990153941572948 56-57 4.8455011944160446E-5 2.4227505972080223E-5 0.0 0.606123744409503 0.0015990153941572948 58-59 4.8455011944160446E-5 4.8455011944160446E-5 0.0 0.7098659249819506 0.0015990153941572948 60-61 7.268251791624068E-5 4.8455011944160446E-5 0.0 0.8295740319899989 0.0015990153941572948 62-63 9.691002388832089E-5 4.8455011944160446E-5 0.0 0.9452846005126541 0.001647470406101455 64-65 9.691002388832089E-5 4.8455011944160446E-5 0.0 1.0683361033448495 0.001647470406101455 66-67 9.691002388832089E-5 4.8455011944160446E-5 0.0 1.222156538761587 0.001647470406101455 68-69 9.691002388832089E-5 4.8455011944160446E-5 0.0 1.3972971794337548 0.001647470406101455 70-71 9.691002388832089E-5 4.8455011944160446E-5 0.0 1.5911899097283126 0.0016716979120735354 72-73 9.691002388832089E-5 4.8455011944160446E-5 0.0 1.7868027929468884 0.0017686079359618564 74-75 9.691002388832089E-5 4.8455011944160446E-5 0.0 1.9940933340440068 0.0018655179598501771 76-77 9.691002388832089E-5 4.8455011944160446E-5 0.0 2.2183189018156093 0.001986655489710578 78-79 9.691002388832089E-5 4.8455011944160446E-5 0.0 2.478061993342281 0.001986655489710578 80-81 9.691002388832089E-5 4.8455011944160446E-5 0.0 2.766660044481701 0.0020351105016547383 82-83 9.691002388832089E-5 4.8455011944160446E-5 0.0 3.0685589963997923 0.0021320205255430597 84-85 9.691002388832089E-5 4.8455011944160446E-5 0.0 3.378501480300615 0.0022289305494313802 86-87 9.691002388832089E-5 4.8455011944160446E-5 0.0 3.7161117760215525 0.0023500680792917813 88-89 9.691002388832089E-5 4.8455011944160446E-5 0.0 4.077368117571241 0.0025196606210963427 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8555 0.0 28.35545 1 GGTATCA 6040 0.0 27.744278 1 ATCAACG 9895 0.0 23.794092 3 TCAACGC 9875 0.0 23.746336 4 CAACGCA 10005 0.0 23.53249 5 TATCAAC 10190 0.0 23.291212 2 AACGCAG 10330 0.0 22.78327 6 TACATGG 6290 0.0 22.067112 2 ACATGGG 6210 0.0 21.512259 3 GTACATG 6650 0.0 21.487234 1 ACGCAGA 11315 0.0 20.632523 7 CGCAGAG 11280 0.0 20.5706 8 GAGTACT 6140 0.0 20.16851 12-13 AGAGTAC 8885 0.0 19.906662 10-11 GTACTTT 6220 0.0 19.720448 14-15 GTAAGGT 860 0.0 19.279606 4 CATGGGG 4050 0.0 18.715157 4 ACTAGCG 65 0.0075138817 18.291292 88-89 GCAGAGT 12580 0.0 18.181364 9 AGTACTT 6540 0.0 17.957441 12-13 >>END_MODULE