FastQCFastQC Report
Fri 27 May 2016
SRR939163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939163_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2063770
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55390.2683923111587047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49860.241596689553584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30890.1496775318955116No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26460.12821196160424855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67900.027.4939461
GTATCAA91200.026.8404051
ATCAACG105850.022.7725933
TCAACGC106850.022.7370994
CAACGCA107350.022.5869945
TATCAAC108900.022.1783642
AACGCAG111000.021.8442696
GAGTACT65500.020.75601412-13
GTATAGC7000.020.4100461
GTACATG62500.020.0399441
AGAGTAC95750.019.72445110-11
ACGCAGA123000.019.6359657
CGCAGAG123350.019.5417828
GTACTTT69450.019.54134414-15
TACATGG61050.019.0423262
GTGTAGC14100.018.9142741
AGTACTT70750.018.67924912-13
ACATGGG61500.018.5172183
GCAGAGT136350.017.400639
GTGTACG1952.2317343E-417.0955921
Produced by FastQC (version 0.11.5)