##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939163_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2063770 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.481184918862084 34.0 31.0 34.0 31.0 34.0 2 32.694978607112226 34.0 31.0 34.0 31.0 34.0 3 32.78899489768724 34.0 31.0 34.0 31.0 34.0 4 36.2529472761015 37.0 35.0 37.0 35.0 37.0 5 36.210632483270906 37.0 37.0 37.0 35.0 37.0 6 36.24126864912272 37.0 37.0 37.0 35.0 37.0 7 36.19750165958416 37.0 37.0 37.0 35.0 37.0 8 36.17821995668122 37.0 37.0 37.0 35.0 37.0 9 37.92803122440969 39.0 38.0 39.0 35.0 39.0 10-11 37.866043696729776 39.0 38.0 39.0 35.0 39.0 12-13 37.798731447787304 39.0 38.0 39.0 35.0 39.0 14-15 39.16708402583622 40.0 39.0 41.0 36.0 41.0 16-17 39.10405374629924 40.0 38.0 41.0 36.0 41.0 18-19 39.12349680439196 40.5 38.5 41.0 36.0 41.0 20-21 39.187597212867715 41.0 39.0 41.0 36.0 41.0 22-23 39.152686345862186 41.0 39.0 41.0 36.0 41.0 24-25 39.098599165604696 41.0 39.0 41.0 35.0 41.0 26-27 38.96225136521996 40.0 38.5 41.0 35.0 41.0 28-29 38.81148674513148 40.0 38.0 41.0 35.0 41.0 30-31 38.61859897178465 40.0 38.0 41.0 34.5 41.0 32-33 38.47813782543597 40.0 38.0 41.0 34.0 41.0 34-35 38.43047892933806 40.0 38.0 41.0 34.0 41.0 36-37 38.32978069261594 40.0 38.0 41.0 34.0 41.0 38-39 38.188976000232586 40.0 38.0 41.0 34.0 41.0 40-41 38.02291461742345 40.0 38.0 41.0 33.0 41.0 42-43 37.86641655804668 40.0 38.0 41.0 33.0 41.0 44-45 37.65847914254011 40.0 37.0 41.0 33.0 41.0 46-47 37.39089966420677 40.0 37.0 41.0 32.5 41.0 48-49 37.08115390765444 40.0 37.0 41.0 31.5 41.0 50-51 36.82045407191693 40.0 36.0 41.0 31.0 41.0 52-53 36.584791425401086 39.0 36.0 41.0 31.0 41.0 54-55 36.3295054681481 39.0 35.0 41.0 30.0 41.0 56-57 36.043835795655525 39.0 35.0 40.5 29.5 41.0 58-59 35.73147540665869 38.5 35.0 40.0 29.0 41.0 60-61 35.36573552285381 38.0 35.0 40.0 28.0 41.0 62-63 34.96401997315593 37.5 34.0 40.0 28.0 41.0 64-65 34.549461907092365 37.0 34.0 40.0 27.0 41.0 66-67 34.18329198505647 36.5 34.0 39.0 26.0 41.0 68-69 33.71204882327004 36.0 33.0 39.0 26.0 40.0 70-71 33.25841130552338 35.5 33.0 38.5 25.5 40.0 72-73 32.783939101740984 35.0 32.5 37.5 24.5 40.0 74-75 32.28623659613232 35.0 32.0 37.0 22.5 39.0 76-77 30.318669716102086 33.5 29.5 35.0 20.0 37.5 78-79 31.29436274391042 34.5 31.0 36.0 20.5 38.0 80-81 31.329443930282928 35.0 31.0 36.0 21.0 37.5 82-83 31.029686932167827 34.5 31.0 35.0 20.0 37.0 84-85 30.64749923683356 34.0 31.0 35.0 18.5 36.5 86-87 30.095779810734722 34.0 30.5 35.0 13.0 36.0 88-89 29.54534638065288 34.0 30.0 35.0 7.0 36.0 90-91 29.083408761635262 34.0 29.0 35.0 2.0 35.0 92-93 28.789273271730863 34.0 29.0 35.0 2.0 35.0 94-95 28.450733366605775 34.0 29.0 35.0 2.0 35.0 96-97 27.877784103848782 34.0 29.0 35.0 2.0 35.0 98-99 27.087964259583188 33.0 27.0 35.0 2.0 35.0 100-101 23.45601423608251 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1023821695902356 1101 2 0.17246005639954376 1101 3 0.17470154543104854 1101 4 0.12402364132776711 1101 5 0.12091041209314568 1101 6 0.0846037917190614 1101 7 0.09016939537650615 1101 8 0.11201945782828204 1101 9 0.16612670860785528 1101 10-11 0.18379449928438163 1101 12-13 0.1691850408591975 1101 14-15 0.183367141639863 1101 16-17 0.21958256207212656 1101 18-19 0.17688059139249646 1101 20-21 0.22296797773789478 1101 22-23 0.25539985611367655 1101 24-25 0.22399907965107246 1101 26-27 0.22222868482782587 1101 28-29 0.13015106324665027 1101 30-31 0.21517963467511692 1101 32-33 0.15144661878010623 1101 34-35 0.3823420515318574 1101 36-37 0.40293888301309977 1101 38-39 0.48402802600153905 1101 40-41 0.43803660861743055 1101 42-43 0.45628616546571266 1101 44-45 0.4259065363795216 1101 46-47 0.3920148692704757 1101 48-49 0.3891255868898895 1101 50-51 0.453449888368624 1101 52-53 0.41851910108032087 1101 54-55 0.27800175119160286 1101 56-57 0.24127345931225364 1101 58-59 0.3335375608414779 1101 60-61 0.3612461883038307 1101 62-63 0.27968660907026077 1101 64-65 0.18699603463377912 1101 66-67 0.2073565343097954 1101 68-69 0.3426500863049924 1101 70-71 0.21883365037596292 1101 72-73 0.0564322225166336 1101 74-75 0.061509674527165714 1101 76-77 0.13721543264945524 1101 78-79 0.21372148900404397 1101 80-81 0.08289018123833358 1101 82-83 0.1404755537332285 1101 84-85 0.1482940216777635 1101 86-87 0.2814502237758134 1101 88-89 0.3911147485602129 1101 90-91 0.40341046428985905 1101 92-93 0.40278943367081865 1101 94-95 0.3965293195892592 1101 96-97 0.2910036660855191 1101 98-99 0.21129075184962076 1101 100-101 -0.18198751922754397 1102 1 0.09335370912540242 1102 2 0.025327038619337827 1102 3 0.11502013272396994 1102 4 0.042739279412600695 1102 5 0.04638616911723403 1102 6 0.06746288511065046 1102 7 0.13471457408881093 1102 8 0.1712542414923348 1102 9 0.14080812131493303 1102 10-11 0.14201526510754547 1102 12-13 0.1293769517761305 1102 14-15 0.16269097803770194 1102 16-17 0.18868915227783845 1102 18-19 0.14823731813562802 1102 20-21 0.20420925451439587 1102 22-23 0.1229714624004643 1102 24-25 0.11523701884641468 1102 26-27 0.09818611549716394 1102 28-29 6.142284872581172E-4 1102 30-31 0.14584912311494236 1102 32-33 0.18876214156669135 1102 34-35 0.21716667681251778 1102 36-37 0.31565415699817123 1102 38-39 0.2314333600630718 1102 40-41 0.3555915269983956 1102 42-43 0.22026683783661127 1102 44-45 0.2391916264942111 1102 46-47 0.24673102316331352 1102 48-49 0.1954217820473616 1102 50-51 0.18947725653416825 1102 52-53 0.217513427192344 1102 54-55 0.07552905867385107 1102 56-57 0.0976045473637086 1102 58-59 -0.015153348801099753 1102 60-61 0.11004602445894562 1102 62-63 0.12140242920498423 1102 64-65 -0.058836691866616775 1102 66-67 -0.06854461260445532 1102 68-69 -0.1082748788021135 1102 70-71 -0.07434964351322293 1102 72-73 -0.0701026096940609 1102 74-75 -0.04132378017275329 1102 76-77 0.18049794372415917 1102 78-79 0.151160047168986 1102 80-81 0.151864330155135 1102 82-83 0.19869616784816202 1102 84-85 0.2670879412117202 1102 86-87 0.4322944801020441 1102 88-89 0.3498663414966785 1102 90-91 0.3074680527357607 1102 92-93 0.12293021891263933 1102 94-95 -0.03628198570839203 1102 96-97 0.07204893582276028 1102 98-99 -0.21126249774076555 1102 100-101 -0.4891388937041512 1103 1 0.20612118487854758 1103 2 0.16397745136764286 1103 3 0.17582469458236716 1103 4 0.09121937986153483 1103 5 0.17913781067265688 1103 6 0.17538927384256908 1103 7 0.18171628354311764 1103 8 0.23544428026718833 1103 9 0.21922349445112133 1103 10-11 0.24719404183884564 1103 12-13 0.2268270897336464 1103 14-15 0.24790999706299743 1103 16-17 0.23627902288830427 1103 18-19 0.18486632631485378 1103 20-21 0.2512423246742088 1103 22-23 0.24212942154043304 1103 24-25 0.15937647221156226 1103 26-27 0.20092526602440586 1103 28-29 0.24131785029371855 1103 30-31 0.22358609910269678 1103 32-33 0.2921944820279734 1103 34-35 0.22430966920370565 1103 36-37 0.3187875648477032 1103 38-39 0.32103326254902953 1103 40-41 0.5182445657619965 1103 42-43 0.36958669513948195 1103 44-45 0.4206248462386739 1103 46-47 0.4224262692945544 1103 48-49 0.40104505240089594 1103 50-51 0.3103880128419618 1103 52-53 0.36121291352765894 1103 54-55 0.4451117186888922 1103 56-57 0.5747380072838482 1103 58-59 0.5623436882039456 1103 60-61 0.5221156357577925 1103 62-63 0.4934767860294542 1103 64-65 0.35504015405113876 1103 66-67 0.3359860034322253 1103 68-69 0.3768197624817091 1103 70-71 0.20828501669353017 1103 72-73 0.213073910249868 1103 74-75 0.28917278997196405 1103 76-77 0.2819226073214196 1103 78-79 0.4080335150062133 1103 80-81 0.35370094324627743 1103 82-83 0.4267735554185563 1103 84-85 0.3061687056792124 1103 86-87 0.4075381616595237 1103 88-89 0.5127082841326214 1103 90-91 0.6252528863435458 1103 92-93 0.6071472042257717 1103 94-95 0.37999898372793695 1103 96-97 0.22613488072377308 1103 98-99 0.3167241212970744 1103 100-101 -0.02417693779020169 1104 1 0.07785279945576917 1104 2 0.019993672818507946 1104 3 0.11682468768157861 1104 4 0.037473747484881414 1104 5 0.1355990178964035 1104 6 0.15406239044517633 1104 7 0.16470584265180577 1104 8 0.12589773100591373 1104 9 0.11724135828938387 1104 10-11 0.17004007607135918 1104 12-13 0.14087116966316415 1104 14-15 0.2615111614558572 1104 16-17 0.29196578924834427 1104 18-19 0.1976077676133059 1104 20-21 0.19872877037767012 1104 22-23 0.17869249588721203 1104 24-25 0.24796935565956346 1104 26-27 0.2132354081116148 1104 28-29 0.14353570585104336 1104 30-31 0.2236668958216086 1104 32-33 0.10711363930381879 1104 34-35 0.18057276632308827 1104 36-37 0.21820427579442025 1104 38-39 0.2820800429866779 1104 40-41 0.25152740833433285 1104 42-43 0.3587720111840369 1104 44-45 0.34151163192092326 1104 46-47 0.30728203133700305 1104 48-49 0.26183259325932084 1104 50-51 0.25326588270691275 1104 52-53 0.11375441601308722 1104 54-55 0.2008733010854442 1104 56-57 0.34331521444318014 1104 58-59 0.27443104987190736 1104 60-61 0.3302536193017076 1104 62-63 0.16368873221251334 1104 64-65 0.061275935553808836 1104 66-67 0.19822702263252268 1104 68-69 0.13714937859091236 1104 70-71 0.2271333672903353 1104 72-73 0.16568735344373664 1104 74-75 0.25402736737925125 1104 76-77 0.19046560957798064 1104 78-79 0.26218221087241034 1104 80-81 0.30820865257591024 1104 82-83 0.35606118713733537 1104 84-85 0.1793874399367894 1104 86-87 0.13545765477368832 1104 88-89 0.1031264258425928 1104 90-91 0.21337507858568472 1104 92-93 0.31681986537640583 1104 94-95 0.15008027500326904 1104 96-97 0.22666537875359793 1104 98-99 0.3538172911277542 1104 100-101 0.225756642131266 1105 1 -0.058716680179138336 1105 2 0.008487089738714815 1105 3 -0.05735135350046505 1105 4 0.019288135985327415 1105 5 0.04538025503119769 1105 6 -0.0023667810607577167 1105 7 0.05424833506238258 1105 8 -0.002904930564319841 1105 9 -0.024707938440663213 1105 10-11 0.04762120565443695 1105 12-13 0.04860248115814869 1105 14-15 -0.016968147669416567 1105 16-17 0.02614807266942165 1105 18-19 0.032873322168470054 1105 20-21 0.06881809743194367 1105 22-23 0.04821310420149416 1105 24-25 -0.029476134922411745 1105 26-27 0.07929545075297284 1105 28-29 0.04697610557747822 1105 30-31 0.1126971508776009 1105 32-33 0.04782324632726187 1105 34-35 0.07265413187284508 1105 36-37 0.10491558646379673 1105 38-39 0.11526248037183251 1105 40-41 0.0011701112740496455 1105 42-43 0.0813248470795358 1105 44-45 0.2034288695376958 1105 46-47 0.15461354387782222 1105 48-49 0.3283102076641029 1105 50-51 0.32566539604656697 1105 52-53 0.16990039961479653 1105 54-55 0.1840934485116179 1105 56-57 0.10863143210375625 1105 58-59 0.19745082644549683 1105 60-61 0.27581923086820836 1105 62-63 0.18791659680892536 1105 64-65 0.22265566562577987 1105 66-67 0.20353362311693957 1105 68-69 0.12468707468591589 1105 70-71 0.30471982425347477 1105 72-73 0.27328704239167934 1105 74-75 -0.007764132911383115 1105 76-77 -0.027697716179876153 1105 78-79 0.11458854102716742 1105 80-81 0.06675337969690531 1105 82-83 0.017113464634146425 1105 84-85 0.22877308981579247 1105 86-87 0.1838652701937491 1105 88-89 0.2489256611543631 1105 90-91 0.21683634155967013 1105 92-93 0.4275806989245119 1105 94-95 0.19371402794448755 1105 96-97 0.15992320832875961 1105 98-99 0.24306426735146047 1105 100-101 -0.1317374754370384 1106 1 0.10788476085397747 1106 2 0.09584948632151935 1106 3 0.0837345199740156 1106 4 6.860366805767626E-4 1106 5 0.021002387718176863 1106 6 0.015621218679591209 1106 7 0.06660655011197036 1106 8 0.04770656985060384 1106 9 0.03472828045135401 1106 10-11 0.06168731778201675 1106 12-13 0.10792315236896854 1106 14-15 0.11221146648751557 1106 16-17 0.07198346759066254 1106 18-19 0.020065710135021675 1106 20-21 0.09430888553446692 1106 22-23 0.07234344629534917 1106 24-25 0.04929721735823023 1106 26-27 0.12983746095402893 1106 28-29 0.10733441651277786 1106 30-31 0.25400886754714946 1106 32-33 0.16082201031721155 1106 34-35 0.08005120353368511 1106 36-37 0.10667115706059604 1106 38-39 0.307400228290966 1106 40-41 0.20569920329821656 1106 42-43 0.2795470288406179 1106 44-45 0.3167958239243731 1106 46-47 0.3741874018748419 1106 48-49 0.3761724004897502 1106 50-51 0.42044089013409547 1106 52-53 0.4787404975151972 1106 54-55 0.4268856568809909 1106 56-57 0.42579877396570964 1106 58-59 0.23163098871322774 1106 60-61 0.1979209704668321 1106 62-63 0.2832651158189421 1106 64-65 0.453532995736353 1106 66-67 0.38640573984541504 1106 68-69 0.24942766475479772 1106 70-71 0.1393605881286888 1106 72-73 0.1441212993433254 1106 74-75 0.21073841762186873 1106 76-77 0.30240436709902596 1106 78-79 0.48509514354018535 1106 80-81 0.4115891390377513 1106 82-83 0.3085100374737628 1106 84-85 0.43553731571500975 1106 86-87 0.48836761656583505 1106 88-89 0.5906914613570677 1106 90-91 0.7049803145091218 1106 92-93 0.8481564819081875 1106 94-95 0.6090985148737289 1106 96-97 0.6604862676004082 1106 98-99 0.7904322177645362 1106 100-101 0.32765155475161123 1107 1 0.031940375907886676 1107 2 0.042050292759164165 1107 3 0.023084580553643264 1107 4 -0.022056544225577568 1107 5 0.027723975159190672 1107 6 0.08856999450235747 1107 7 0.06405497081277645 1107 8 -0.011861699671165127 1107 9 -0.024214577011761662 1107 10-11 0.08431029340852092 1107 12-13 0.007495732266313837 1107 14-15 0.044612447581982906 1107 16-17 0.05071741959365994 1107 18-19 0.06057012088222535 1107 20-21 0.09335675617835903 1107 22-23 -0.005117594736773867 1107 24-25 -0.007008209944686428 1107 26-27 0.06080445244434429 1107 28-29 2.2782489896400193E-4 1107 30-31 0.08358752069234043 1107 32-33 0.022027025230293873 1107 34-35 0.010429084887285 1107 36-37 0.007368969162868666 1107 38-39 0.024596283125461582 1107 40-41 0.08505174005104976 1107 42-43 0.08786613012221522 1107 44-45 -0.05297020318310075 1107 46-47 -0.27348601431688735 1107 48-49 -0.2804039028384082 1107 50-51 -0.1289313795792424 1107 52-53 -0.10461266388544033 1107 54-55 -0.16351415338096587 1107 56-57 -0.12541750600694712 1107 58-59 -0.2671654433555588 1107 60-61 -0.06582637630848609 1107 62-63 -0.11811988529687056 1107 64-65 0.051833123213413046 1107 66-67 -0.13016831658482886 1107 68-69 -0.18248852646284064 1107 70-71 0.004281518721448663 1107 72-73 -0.19426967719620336 1107 74-75 -0.21981957221461101 1107 76-77 -0.21303694199158585 1107 78-79 -0.2294196640774615 1107 80-81 -0.1577493151168703 1107 82-83 -0.1881797367461182 1107 84-85 -0.3314409021128242 1107 86-87 -0.31918761585308175 1107 88-89 -0.2200565569401185 1107 90-91 -0.20763548225310302 1107 92-93 -0.20167268252092185 1107 94-95 -0.17222316550020622 1107 96-97 -0.09752957888907687 1107 98-99 -0.19161183264342085 1107 100-101 -0.49071681014868673 1108 1 0.0036211076433332323 1108 2 -0.017721851171742742 1108 3 0.01219417079978058 1108 4 0.029967594130127395 1108 5 0.021151316714579593 1108 6 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76-77 0.07558154053684163 2316 78-79 0.16062671144118212 2316 80-81 0.24409263127056846 2316 82-83 0.037374866004881824 2316 84-85 -0.09642229037897465 2316 86-87 -0.13843211020226676 2316 88-89 -0.35768763699748973 2316 90-91 -0.34293490428954954 2316 92-93 -0.23651853967971093 2316 94-95 0.24219179541130842 2316 96-97 -0.060400804148738274 2316 98-99 -0.028293387415370574 2316 100-101 0.2854995858659457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 13.0 5 42.0 6 53.0 7 172.0 8 409.0 9 849.0 10 1444.0 11 2321.0 12 3594.0 13 5009.0 14 6422.0 15 8613.0 16 10869.0 17 14893.0 18 15169.0 19 11130.0 20 10579.0 21 10673.0 22 11425.0 23 12356.0 24 14318.0 25 16854.0 26 19335.0 27 23849.0 28 28688.0 29 34813.0 30 42870.0 31 52584.0 32 66379.0 33 85104.0 34 118292.0 35 174730.0 36 286398.0 37 485667.0 38 440880.0 39 46966.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.34749696340822 19.207111605566908 15.15991095905731 23.285480471967563 2 15.670690047825097 20.180155734408388 40.17594014836925 23.973214069397265 3 19.690469383700705 23.70491866826245 29.61177844430339 26.99283350373346 4 13.411814301012226 16.8232409619289 36.33001739534929 33.43492734170959 5 14.110341753199243 36.88264680657244 34.15002640798151 14.856985032246811 6 32.74434651148142 35.67466335880452 17.095751949102855 14.485238180611212 7 28.839017913817916 32.155521206335976 20.5061610547687 18.499299825077404 8 26.01326698227031 35.751125367652406 19.387189463942203 18.84841818613508 9 25.831851417551377 17.490757206471653 20.32377638981088 36.35361498616609 10-11 25.16256656507266 26.526187511205222 26.9146997969735 21.39654612674862 12-13 26.06300605203099 23.6493407695625 27.516050722706503 22.771602455700005 14-15 22.684693450350434 25.272296370092413 25.811728586491352 26.23128159306581 16-17 22.279777179938442 28.284226409681896 26.7638710140096 22.672125396370063 18-19 22.322774496564296 26.991877482412036 28.717385521344685 21.967962499678986 20-21 22.838203869617253 26.080328718801027 29.0318446338497 22.049622777732015 22-23 22.46519718767111 26.011546829346294 28.96945880597159 22.553797177011003 24-25 22.523972033556245 26.20075793733668 29.017874664628962 22.257395364478118 26-27 22.2135298430187 26.34014601581878 28.954805065926138 22.491519075236376 28-29 22.165260663736756 26.907383090169933 28.79305833498888 22.134297911104436 30-31 22.42063093064754 26.363093428179273 28.674842121967973 22.541433519205214 32-33 22.22822795175819 26.358290894818705 28.691593539977806 22.721887613445297 34-35 22.79676817899316 26.483583449335306 28.52754425944704 22.192104112224488 36-37 22.779732553695617 26.173376493234596 28.610342139236 22.436548813833788 38-39 22.42796678555279 26.493237352052688 28.622476698949605 22.456319163444917 40-41 22.747448245247597 26.404543388311602 28.583336321809444 22.264672044631357 42-43 22.459963146347285 26.483916809118192 28.23929917485132 22.81682086968321 44-45 22.602119249261946 26.573428098284513 28.40048408140224 22.423968571051304 46-47 22.968975053816067 26.830600232344874 27.76618206971073 22.434242644128325 48-49 22.836390824208188 26.874875984642564 27.713710411087746 22.575022780061506 50-51 22.681026800964645 27.057776330587384 27.803741600925296 22.457455267522676 52-53 23.01516157323733 26.989587017933196 27.515275442515396 22.479975966314075 54-55 22.70991922549509 26.917074092558764 27.59888456562505 22.774122116321102 56-57 22.73613338695688 27.10849561724417 27.538654985778454 22.616716010020497 58-59 22.777595524881075 26.857473335216703 27.61311484306895 22.751816296833276 60-61 22.529204969175865 26.971209726485014 27.91465144120977 22.584933863129354 62-63 22.53214584331247 26.99361650216688 27.958324944195578 22.515912710325072 64-65 22.34095287751099 27.095067760867714 27.76720091019747 22.79677845142383 66-67 22.405395006798514 27.033545801056945 27.596930928445513 22.96412826369903 68-69 22.657854944818517 26.815624726672922 27.7672209083678 22.759299420140763 70-71 22.631898878192978 26.895143520215566 27.442273685251035 23.030683916340422 72-73 22.55733439288293 26.87625074499581 27.58803064294955 22.97838421917171 74-75 22.455530412788246 27.259723709521893 27.259190704390505 23.02555517329935 76-77 22.54240485630022 26.9265404955118 27.464284162128212 23.06677048605977 78-79 22.631996681791136 27.018181342440318 27.434459560298563 22.915362415469986 80-81 22.734031317967915 27.00366113639871 27.268427853129097 22.993879692504276 82-83 22.73329660420292 27.01747347192523 27.42493423402077 22.82429568985108 84-85 22.57468128715894 27.14818027202644 27.288118346521173 22.98902009429345 86-87 22.64370740523981 27.095087985412608 27.232681536536507 23.028523072811073 88-89 22.567280431723454 27.102809194434883 27.020663031672616 23.309247342169055 90-91 22.523304133998316 27.052277876487178 27.113595263894613 23.310822725619893 92-93 22.671833586106978 26.927685740174535 27.236586441318554 23.16389423239993 94-95 22.440049036472086 26.694592905217153 27.36455612786309 23.500801930447675 96-97 22.523755069605624 26.4658125663228 27.466457017981654 23.543975346089923 98-99 22.299010923835514 26.70830296780996 27.13992142436271 23.85276468399181 100-101 22.582908500104907 26.000084312497002 27.700773979031386 23.716233208366706 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 318.0 1 229.0 2 195.0 3 254.0 4 209.0 5 202.0 6 354.0 7 811.5 8 1361.0 9 1597.0 10 1465.5 11 1282.0 12 1339.0 13 1472.5 14 1628.5 15 1773.5 16 1892.0 17 2089.0 18 2329.0 19 2621.5 20 3015.5 21 3731.5 22 4624.0 23 5696.0 24 7261.0 25 9119.5 26 11715.5 27 14964.5 28 17668.5 29 20293.0 30 23742.5 31 28068.0 32 33610.0 33 37033.0 34 38718.5 35 41599.0 36 45418.0 37 49217.0 38 52430.0 39 55316.0 40 57985.5 41 61149.5 42 62884.5 43 64154.5 44 66923.0 45 70764.5 46 73405.0 47 76425.5 48 81477.0 49 81404.5 50 76928.5 51 74657.5 52 75257.5 53 76101.5 54 75087.0 55 70088.0 56 64004.5 57 58421.0 58 53681.0 59 47724.5 60 38953.0 61 32260.5 62 26305.5 63 19355.0 64 13253.0 65 9480.5 66 7171.0 67 5558.0 68 4196.0 69 3327.0 70 2668.5 71 1772.0 72 1236.5 73 956.0 74 707.5 75 531.5 76 380.5 77 224.0 78 142.5 79 95.5 80 60.0 81 38.0 82 25.0 83 15.0 84 6.5 85 3.5 86 4.0 87 4.5 88 4.5 89 3.5 90 2.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3482946258546253 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012598303105481716 16-17 0.0020351105016547387 18-19 4.8455011944160446E-5 20-21 0.0 22-23 0.0 24-25 5.330051313857649E-4 26-27 0.0010175552508273694 28-29 0.0 30-31 0.003512988365951632 32-33 0.0 34-35 0.011023515217296501 36-37 0.02856422954108258 38-39 0.021853210386816358 40-41 0.013955043439918208 42-43 0.00981213991869249 44-45 0.00327071330623083 46-47 0.0013325128284644122 48-49 6.54142661246166E-4 50-51 4.8455011944160446E-5 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.004506316110806922 60-61 0.010102869990357453 62-63 0.00433672356900236 64-65 0.0020351105016547387 66-67 0.004070221003309477 68-69 0.003852173449560755 70-71 7.268251791624066E-5 72-73 0.0 74-75 0.0 76-77 0.2143892003469379 78-79 2.9073007166496265E-4 80-81 0.0032222582942866697 82-83 6.299151552740858E-4 84-85 0.0 86-87 0.10967791953560715 88-89 0.4015709114872297 90-91 0.3911530839192352 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0012598303105481716 100-101 9.206452269390485E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2063770.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.63914456865681 #Duplication Level Percentage of deduplicated Percentage of total 1 78.51588466118808 46.82620196244579 2 12.776526666977423 15.23962241954331 3 3.8485435728956205 6.885715395680908 4 1.6253976150561271 3.877492933835294 5 0.8560875324734157 2.5528164056303355 6 0.5252787892999122 1.8796306592343888 7 0.34473945902717895 1.4391976504809727 8 0.22556685709847288 1.0762091520314698 9 0.18492773107416416 0.9926038519457188 >10 0.9967326454180402 10.844271403897377 >50 0.06791247129847912 2.746521075097915 >100 0.028659153801235314 3.1562000654535445 >500 0.0026037178378092744 1.0681078807944184 >1k 0.0010577603716100054 1.1466231027800229 >5k 8.136618243153886E-5 0.2687860411485271 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5539 0.2683923111587047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4986 0.241596689553584 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3089 0.1496775318955116 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2646 0.12821196160424855 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.8455011944160446E-5 2 0.0 0.0 0.0 0.0 4.8455011944160446E-5 3 0.0 0.0 0.0 0.0 4.8455011944160446E-5 4 0.0 0.0 0.0 0.0 9.691002388832089E-5 5 0.0 0.0 0.0 0.0 9.691002388832089E-5 6 0.0 0.0 0.0 0.0 1.9382004777664178E-4 7 0.0 0.0 0.0 0.0 1.9382004777664178E-4 8 0.0 0.0 0.0 0.0 1.9382004777664178E-4 9 0.0 0.0 0.0 0.0 1.9382004777664178E-4 10-11 0.0 0.0 0.0 0.0 2.18047553748722E-4 12-13 4.8455011944160446E-5 0.0 0.0 0.0 5.087776254136847E-4 14-15 4.8455011944160446E-5 0.0 0.0 0.0 6.299151552740858E-4 16-17 4.8455011944160446E-5 0.0 0.0 0.0 7.995076970786473E-4 18-19 4.8455011944160446E-5 0.0 0.0 0.0 8.964177209669682E-4 20-21 4.8455011944160446E-5 0.0 0.0 0.0 9.93327744855289E-4 22-23 4.8455011944160446E-5 0.0 0.0 4.8455011944160446E-5 0.0010175552508273694 24-25 4.8455011944160446E-5 0.0 0.0 1.9382004777664178E-4 0.0010175552508273694 26-27 4.8455011944160446E-5 0.0 0.0 4.8455011944160443E-4 0.0011144652747156901 28-29 9.691002388832089E-5 0.0 0.0 0.0016716979120735354 0.0011629202866598506 30-31 9.691002388832089E-5 0.0 0.0 0.005523871361634291 0.0011629202866598506 32-33 9.691002388832089E-5 0.0 0.0 0.014827233654913096 0.0012598303105481716 34-35 9.691002388832089E-5 0.0 0.0 0.026868304123036965 0.0012598303105481716 36-37 9.691002388832089E-5 0.0 0.0 0.048648831991937085 0.0012598303105481716 38-39 9.691002388832089E-5 0.0 0.0 0.07777029417037751 0.001284057816520252 40-41 9.691002388832089E-5 0.0 0.0 0.11088929483421117 0.0013567403344364923 42-43 9.691002388832089E-5 0.0 0.0 0.14679445868483407 0.0014536503583248133 44-45 9.691002388832089E-5 0.0 0.0 0.19130038715554543 0.0015263328762410541 46-47 9.691002388832089E-5 0.0 0.0 0.2486711212974314 0.001623242900129375 48-49 9.691002388832089E-5 0.0 0.0 0.3083192410006929 0.0017201529240176957 50-51 9.691002388832089E-5 0.0 0.0 0.3776099080808424 0.0017928354419339365 52-53 9.691002388832089E-5 0.0 0.0 0.44384790940850966 0.0018897454658222573 54-55 9.691002388832089E-5 0.0 0.0 0.5180325326950193 0.0018897454658222573 56-57 9.691002388832089E-5 0.0 0.0 0.6120594833726627 0.001962427983738498 58-59 9.691002388832089E-5 0.0 0.0 0.7167707641839933 0.001986655489710578 60-61 9.691002388832089E-5 0.0 0.0 0.8375448814548132 0.001986655489710578 62-63 9.691002388832089E-5 0.0 0.0 0.9539580476506587 0.0020351105016547387 64-65 9.691002388832089E-5 0.0 0.0 1.0788266134307603 0.0021077930195709796 66-67 9.691002388832089E-5 0.0 0.0 1.2332527364967996 0.00215624803151514 68-69 9.691002388832089E-5 0.0 0.0 1.4099439375511804 0.0022289305494313802 70-71 9.691002388832089E-5 0.0 0.0 1.6049511331204545 0.0022773855613755405 72-73 9.691002388832089E-5 0.0 0.0 1.802066121709299 0.002398523091235942 74-75 9.691002388832089E-5 0.0 0.0 2.01020462551544 0.002422750597208022 76-77 9.691002388832089E-5 0.0 0.0 2.235811161127451 0.002422750597208022 78-79 9.691002388832089E-5 0.0 0.0 2.497371315602029 0.0024469781031801023 80-81 9.691002388832089E-5 0.0 0.0 2.7882225247968524 0.0024712056091521824 82-83 9.691002388832089E-5 0.0 0.0 3.0914297620374365 0.0026650256569288244 84-85 9.691002388832089E-5 0.0 0.0 3.4037707690294945 0.0026650256569288244 86-87 9.691002388832089E-5 4.8455011944160446E-5 0.0 3.7423743924952877 0.0027861631867892255 88-89 9.691002388832089E-5 4.8455011944160446E-5 0.0 4.105859664594407 0.0029315282226217067 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6790 0.0 27.493946 1 GTATCAA 9120 0.0 26.840405 1 ATCAACG 10585 0.0 22.772593 3 TCAACGC 10685 0.0 22.737099 4 CAACGCA 10735 0.0 22.586994 5 TATCAAC 10890 0.0 22.178364 2 AACGCAG 11100 0.0 21.844269 6 GAGTACT 6550 0.0 20.756014 12-13 GTATAGC 700 0.0 20.410046 1 GTACATG 6250 0.0 20.039944 1 AGAGTAC 9575 0.0 19.724451 10-11 ACGCAGA 12300 0.0 19.635965 7 CGCAGAG 12335 0.0 19.541782 8 GTACTTT 6945 0.0 19.541344 14-15 TACATGG 6105 0.0 19.042326 2 GTGTAGC 1410 0.0 18.914274 1 AGTACTT 7075 0.0 18.679249 12-13 ACATGGG 6150 0.0 18.517218 3 GCAGAGT 13635 0.0 17.40063 9 GTGTACG 195 2.2317343E-4 17.095592 1 >>END_MODULE