FastQCFastQC Report
Fri 27 May 2016
SRR939162_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939162_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031157
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52140.25670098372503947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43910.21618220551144005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27600.13588314443442825No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21870.10767262205728066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87000.028.8758351
GGTATCA59900.026.559271
TCAACGC100550.024.3515954
ATCAACG100600.024.3394953
CAACGCA101150.024.2071485
TATCAAC102200.024.190152
AACGCAG103350.023.6795946
GTACATG63600.022.1020581
TAAGGTG10550.021.9969565
TACATGG62700.021.6031682
ACGCAGA114400.021.3096057
CGCAGAG114700.021.1300628
AGAGTAC86250.020.745910-11
GAGTACT60100.020.71582612-13
GTGTAAG9400.020.713531
ACATGGG62500.020.4598643
GTAAGGT9550.020.3329054
GTACTTT63150.019.56609514-15
AGTACTT64350.019.49477812-13
GCAGAGT126000.018.821819
Produced by FastQC (version 0.11.5)