##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939162_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2031157 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96031818318328 33.0 31.0 34.0 30.0 34.0 2 32.07519261189559 34.0 31.0 34.0 30.0 34.0 3 32.13549617287093 34.0 31.0 34.0 30.0 34.0 4 35.57777020683285 37.0 35.0 37.0 33.0 37.0 5 35.608027345990486 37.0 35.0 37.0 33.0 37.0 6 35.684575835349015 37.0 36.0 37.0 35.0 37.0 7 35.60686446197906 37.0 35.0 37.0 33.0 37.0 8 35.59907579768575 37.0 35.0 37.0 33.0 37.0 9 37.25886132878945 39.0 37.0 39.0 34.0 39.0 10-11 37.15548453418421 39.0 37.0 39.0 34.0 39.0 12-13 37.053889482693855 39.0 37.0 39.0 33.0 39.0 14-15 38.318205584304906 40.0 38.0 41.0 33.0 41.0 16-17 38.211965889392104 40.0 38.0 41.0 33.0 41.0 18-19 38.093409322863764 40.0 38.0 41.0 33.0 41.0 20-21 38.06289715664521 40.0 38.0 41.0 33.0 41.0 22-23 38.10721032396806 40.0 38.0 41.0 33.0 41.0 24-25 38.05151201999648 40.0 38.0 41.0 33.0 41.0 26-27 37.92755754478851 40.0 38.0 41.0 33.0 41.0 28-29 37.78100166555318 40.0 38.0 41.0 32.5 41.0 30-31 37.56722449323218 40.0 37.5 41.0 32.0 41.0 32-33 37.355662806961746 40.0 37.0 41.0 31.5 41.0 34-35 37.03679429999749 40.0 37.0 41.0 30.5 41.0 36-37 36.773533015911624 40.0 36.5 41.0 30.0 41.0 38-39 36.57960930641994 40.0 36.0 41.0 30.0 41.0 40-41 36.381537960876486 40.0 36.0 41.0 30.0 41.0 42-43 36.17375367832226 39.0 35.5 41.0 29.5 41.0 44-45 35.90444264032766 39.0 35.0 41.0 28.0 41.0 46-47 35.78690273573141 39.0 35.0 41.0 27.0 41.0 48-49 35.65687241311233 39.0 35.0 41.0 26.5 41.0 50-51 34.53741365143118 38.0 34.0 40.0 25.0 40.5 52-53 34.68621406420085 38.5 34.5 39.5 25.0 40.5 54-55 35.2128515914821 39.0 35.0 40.5 26.0 41.0 56-57 35.10342799694952 39.0 35.0 41.0 26.0 41.0 58-59 34.88375221610146 39.0 35.0 41.0 25.0 41.0 60-61 34.57181818047546 38.0 34.5 40.0 24.0 41.0 62-63 34.18229093073553 38.0 34.0 40.0 23.0 41.0 64-65 33.80477555403152 37.0 34.0 40.0 22.0 41.0 66-67 33.40404951463624 36.5 33.5 39.5 21.5 41.0 68-69 32.97973691841645 36.0 33.0 39.0 20.0 41.0 70-71 32.53842859020745 35.5 32.5 39.0 18.5 40.5 72-73 32.09349178817787 35.0 32.0 38.0 16.5 40.0 74-75 31.64890552527451 35.0 32.0 37.0 12.5 39.5 76-77 31.201863519166665 35.0 31.0 37.0 8.0 39.0 78-79 30.772293820714005 35.0 31.0 36.0 7.0 39.0 80-81 30.321046083586843 35.0 31.0 36.0 2.0 37.5 82-83 29.904077577459546 34.0 30.0 35.0 2.0 37.0 84-85 29.513164664277554 34.0 30.0 35.0 2.0 37.0 86-87 29.094353366086423 34.0 29.0 35.0 2.0 36.0 88-89 28.658168718617027 34.0 29.0 35.0 2.0 36.0 90-91 28.230730810075244 34.0 29.0 35.0 2.0 35.5 92-93 27.801885821726238 34.0 27.5 35.0 2.0 35.0 94-95 27.291874532593983 34.0 26.5 35.0 2.0 35.0 96-97 26.66663901411855 33.5 25.0 35.0 2.0 35.0 98-99 25.909546874023032 33.0 24.0 35.0 2.0 35.0 100-101 22.97114329419144 30.0 8.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13560675123759935 1101 2 0.29681553753315626 1101 3 0.2837265092594592 1101 4 0.25196839889594 1101 5 0.23080220942807017 1101 6 0.1458962641831434 1101 7 0.3046100430775667 1101 8 0.2346610091732444 1101 9 0.3065551720242894 1101 10-11 0.23498062592823032 1101 12-13 0.27609404906998236 1101 14-15 0.30194279879816577 1101 16-17 0.42679275084714874 1101 18-19 0.6427538218203708 1101 20-21 0.572476096625536 1101 22-23 0.5161765285944924 1101 24-25 0.5999945639419053 1101 26-27 0.5755572009155614 1101 28-29 0.5794284540826311 1101 30-31 0.5660298036350824 1101 32-33 0.4761395340885386 1101 34-35 0.47616916687572086 1101 36-37 0.5128625178869441 1101 38-39 0.4325727148808838 1101 40-41 0.37013507124179057 1101 42-43 0.3828583705172406 1101 44-45 0.3658396541310296 1101 46-47 0.4936277114169627 1101 48-49 0.4360087320769992 1101 50-51 0.4059117845674294 1101 52-53 0.3526503183434144 1101 54-55 0.5142781867850346 1101 56-57 0.4969862537838381 1101 58-59 0.23402791513350962 1101 60-61 0.18060868727894075 1101 62-63 0.21528835025658566 1101 64-65 0.16011566012684852 1101 66-67 0.09748586862050246 1101 68-69 0.001983848961536694 1101 70-71 -0.06515876616975902 1101 72-73 -0.14568404875511476 1101 74-75 -0.22233614259065604 1101 76-77 -0.27681284891300706 1101 78-79 -0.19985654817093845 1101 80-81 -0.2954208222843455 1101 82-83 -0.24160733546937507 1101 84-85 -0.30093280115108456 1101 86-87 -0.4927295856740521 1101 88-89 -0.4925946422610856 1101 90-91 -0.4293274940316927 1101 92-93 -0.6073038798961825 1101 94-95 -0.7064089749407145 1101 96-97 -0.7831172608134658 1101 98-99 -0.7279120553208536 1101 100-101 -0.935037328974964 1102 1 0.397197601557739 1102 2 0.40549913951420535 1102 3 0.40647606205132547 1102 4 0.3283564874213596 1102 5 0.4196368726830073 1102 6 0.3997532628249729 1102 7 0.38968499422446 1102 8 0.4600271129113267 1102 9 0.42940674089273045 1102 10-11 0.47197220411371177 1102 12-13 0.5771194397738029 1102 14-15 0.610497686191465 1102 16-17 0.6362180355317193 1102 18-19 0.6236624493135068 1102 20-21 0.5968556790763202 1102 22-23 0.6838481827496636 1102 24-25 0.5607779376458453 1102 26-27 0.6489613192012698 1102 28-29 0.7036339958154372 1102 30-31 0.8039618021719903 1102 32-33 0.6634978697543588 1102 34-35 0.7433542753694908 1102 36-37 0.6775122686093695 1102 38-39 0.5693330135813781 1102 40-41 0.36027486232191563 1102 42-43 0.4604585456233181 1102 44-45 0.5368547560615582 1102 46-47 0.7101608693859518 1102 48-49 0.877736729452991 1102 50-51 0.6455259184428783 1102 52-53 0.6597009528608098 1102 54-55 0.6507639137351262 1102 56-57 0.7751874843688995 1102 58-59 0.6009952386739457 1102 60-61 0.5423857815059634 1102 62-63 0.4384055806104996 1102 64-65 0.5771828851701102 1102 66-67 0.5770372948924134 1102 68-69 0.564072092222105 1102 70-71 0.5350298990047548 1102 72-73 0.75089489377077 1102 74-75 0.6095739696237956 1102 76-77 0.6905092387620648 1102 78-79 0.4642464803183408 1102 80-81 0.45274958717783775 1102 82-83 0.29402081076615616 1102 84-85 0.1855158481205521 1102 86-87 0.08253907011240713 1102 88-89 0.08610704820341297 1102 90-91 0.23797338333885065 1102 92-93 0.10538769483494903 1102 94-95 0.1702660176582782 1102 96-97 0.010899528579187745 1102 98-99 0.1755907441487956 1102 100-101 -0.35124984654126123 1103 1 0.29211746932530147 1103 2 0.42162464248064424 1103 3 0.4877498328193468 1103 4 0.4103077280639411 1103 5 0.5492734564384492 1103 6 0.41133968578085245 1103 7 0.43720662341369376 1103 8 0.4939095249079273 1103 9 0.4844840543153879 1103 10-11 0.3892555849527497 1103 12-13 0.48496617806053166 1103 14-15 0.565422051314286 1103 16-17 0.6747013183972186 1103 18-19 0.6754654842638814 1103 20-21 0.4981083135486699 1103 22-23 0.5992792711319268 1103 24-25 0.6738528195663847 1103 26-27 0.7170081834266853 1103 28-29 0.652668641057879 1103 30-31 0.6403189061937198 1103 32-33 0.5960031418841609 1103 34-35 0.6869447956325985 1103 36-37 0.592740307135017 1103 38-39 0.5644711241124796 1103 40-41 0.5955812987953379 1103 42-43 0.5268404010244296 1103 44-45 0.5120090978408101 1103 46-47 0.6665964032064124 1103 48-49 0.5554798852395351 1103 50-51 0.6068750995388186 1103 52-53 0.644018496718779 1103 54-55 0.5821481914015862 1103 56-57 0.7207446420276398 1103 58-59 0.6109502555660953 1103 60-61 0.5562692955457109 1103 62-63 0.4888789973232761 1103 64-65 0.48635589734386286 1103 66-67 0.5348836668931298 1103 68-69 0.43353938176610285 1103 70-71 0.4894341379850431 1103 72-73 0.4855327786378396 1103 74-75 0.4237465629574615 1103 76-77 0.4176844441927763 1103 78-79 0.5402270996474776 1103 80-81 0.34197147783158854 1103 82-83 -0.03541555786791406 1103 84-85 -0.12313378911296269 1103 86-87 -0.04883163904598575 1103 88-89 0.00755940092219376 1103 90-91 0.10035733835510285 1103 92-93 0.07562026734445126 1103 94-95 0.042475515422815135 1103 96-97 -0.09175865681440953 1103 98-99 0.09181476212802053 1103 100-101 0.004656897676117211 1104 1 0.1560100025992277 1104 2 0.24116844798874837 1104 3 0.2681350649460441 1104 4 0.33569272639990544 1104 5 0.2638919787159537 1104 6 0.2769980267467602 1104 7 0.20373494097302824 1104 8 0.2846255571765468 1104 9 0.2535593156796807 1104 10-11 0.2839768651358128 1104 12-13 0.3484667680383353 1104 14-15 0.47174485306248926 1104 16-17 0.32923424575981386 1104 18-19 0.3678312539992632 1104 20-21 0.37393655161915973 1104 22-23 0.44835634781254186 1104 24-25 0.3789766874003462 1104 26-27 0.38759703353381525 1104 28-29 0.5049527765684942 1104 30-31 0.43350624408734717 1104 32-33 0.4513389341459657 1104 34-35 0.40159219794244905 1104 36-37 0.5575548010605758 1104 38-39 0.27183113159378536 1104 40-41 0.24366297702776762 1104 42-43 0.4060819838702372 1104 44-45 0.32340598374818086 1104 46-47 0.571469702378792 1104 48-49 0.5046634054679373 1104 50-51 0.42852439692888566 1104 52-53 0.49173133568097427 1104 54-55 0.41596835956589473 1104 56-57 0.5118347394771234 1104 58-59 0.4993096771953134 1104 60-61 0.5429624768325496 1104 62-63 0.4707629656047416 1104 64-65 0.4792129026770482 1104 66-67 0.5143980972826228 1104 68-69 0.33588636572805797 1104 70-71 0.20547053232675694 1104 72-73 0.15304246720107528 1104 74-75 0.1905458027249871 1104 76-77 0.06383391073732625 1104 78-79 0.2921930047064336 1104 80-81 0.22383151694340242 1104 82-83 0.21240124921071768 1104 84-85 0.19118273054058577 1104 86-87 0.30957093760299514 1104 88-89 0.18963837818398233 1104 90-91 0.24372331987738605 1104 92-93 0.23768400695108127 1104 94-95 0.0948391397843622 1104 96-97 -0.008454891133240494 1104 98-99 -0.146178708234185 1104 100-101 -0.5383592228828888 1105 1 0.04075672294105104 1105 2 0.1093509082179267 1105 3 0.12187025572239207 1105 4 0.21696556924115384 1105 5 0.1421491251043605 1105 6 0.14708471919163202 1105 7 0.08242554958577841 1105 8 0.22221052813193154 1105 9 0.10842274418048703 1105 10-11 0.11750750764866069 1105 12-13 0.16613366423693776 1105 14-15 0.20010352318978875 1105 16-17 0.17575992685167563 1105 18-19 0.23453367467831754 1105 20-21 0.14737705870815887 1105 22-23 0.2682563248593439 1105 24-25 0.36005398669232846 1105 26-27 0.2750195665069626 1105 28-29 0.25519246087380765 1105 30-31 0.3806874154064488 1105 32-33 0.2190795454071477 1105 34-35 0.18487035533072316 1105 36-37 0.11863501364246076 1105 38-39 0.24094849303595112 1105 40-41 0.40454367339233244 1105 42-43 0.2488883648579332 1105 44-45 0.2455085845215237 1105 46-47 0.2840776265273206 1105 48-49 0.4329951497340474 1105 50-51 0.3272615298985002 1105 52-53 0.24653826401404189 1105 54-55 0.20380846409119613 1105 56-57 0.19132694421959684 1105 58-59 0.32314789249626585 1105 60-61 0.2349805039173205 1105 62-63 0.35121081771556106 1105 64-65 0.32205680330737607 1105 66-67 0.3999901131026746 1105 68-69 0.28786387159878046 1105 70-71 0.1948582117589268 1105 72-73 0.3185491147989268 1105 74-75 0.19965027506638933 1105 76-77 0.29346162760085903 1105 78-79 0.2516007240418503 1105 80-81 0.13516774591033354 1105 82-83 0.1482766486845577 1105 84-85 0.02377008509679257 1105 86-87 0.056138552413102616 1105 88-89 0.0416158840547034 1105 90-91 0.19222032713412673 1105 92-93 0.16316867245243216 1105 94-95 -0.023188827798666267 1105 96-97 0.013147594874137525 1105 98-99 0.018664062392787173 1105 100-101 0.10510132410935924 1106 1 0.3077684680115631 1106 2 0.3024665376758975 1106 3 0.3478063936635323 1106 4 0.3400212610613309 1106 5 0.3586094213530373 1106 6 0.36555374389406126 1106 7 0.2850170207362055 1106 8 0.2621031704062915 1106 9 0.39748962285610645 1106 10-11 0.44475390039525564 1106 12-13 0.3505696132871208 1106 14-15 0.34207537561802326 1106 16-17 0.43794889110899504 1106 18-19 0.4380719969883202 1106 20-21 0.46583001358295917 1106 22-23 0.5000338451439816 1106 24-25 0.5619596709004853 1106 26-27 0.5368712053474525 1106 28-29 0.5470093945251548 1106 30-31 0.5028688522181923 1106 32-33 0.40828865615363696 1106 34-35 0.40367050306037555 1106 36-37 0.3310008869671037 1106 38-39 0.4255417572408362 1106 40-41 0.6647598548629787 1106 42-43 0.58378596904398 1106 44-45 0.5305513810098503 1106 46-47 0.4313171402599707 1106 48-49 0.42762289358662997 1106 50-51 0.3713361333684517 1106 52-53 0.464030694279451 1106 54-55 0.4332111739716069 1106 56-57 0.532189414687771 1106 58-59 0.38036394991911493 1106 60-61 0.4813677529319804 1106 62-63 0.34913992440230146 1106 64-65 0.32965501250833995 1106 66-67 0.31220191343083314 1106 68-69 0.41498494788253026 1106 70-71 0.31407180514154476 1106 72-73 0.41984879734935276 1106 74-75 0.31626675876255916 1106 76-77 0.17827042484474376 1106 78-79 0.33515861207180464 1106 80-81 0.4093892050506831 1106 82-83 0.46837619703782707 1106 84-85 0.5415187868238789 1106 86-87 0.5175171180823135 1106 88-89 0.5838916535739038 1106 90-91 0.527261713988441 1106 92-93 0.3082790749875137 1106 94-95 0.34256185869309164 1106 96-97 0.3106875016127546 1106 98-99 0.4134596936550885 1106 100-101 0.41902949479912266 1107 1 0.05913777514066609 1107 2 0.07203675115881225 1107 3 0.0341518330790791 1107 4 0.05274085524935401 1107 5 0.101992354349008 1107 6 0.06618113847437712 1107 7 0.07387774476453757 1107 8 0.1845068010139741 1107 9 0.2891782605479776 1107 10-11 0.23701581755234002 1107 12-13 0.15497227409569092 1107 14-15 0.1931519851505641 1107 16-17 0.14715397765937155 1107 18-19 0.20255468650682928 1107 20-21 0.25626618790124667 1107 22-23 0.17794303289530689 1107 24-25 0.25402979175757423 1107 26-27 0.24712884576076277 1107 28-29 0.26023011469159485 1107 30-31 0.325762196221298 1107 32-33 0.25627156324141964 1107 34-35 0.1691778910852193 1107 36-37 0.20657303734384413 1107 38-39 0.3319531678766552 1107 40-41 0.37798192723118973 1107 42-43 0.2730928655705256 1107 44-45 0.23183134816756734 1107 46-47 0.48514721122622717 1107 48-49 0.4090044273242057 1107 50-51 0.28082907663304724 1107 52-53 0.3905682761586462 1107 54-55 0.299121977874492 1107 56-57 0.30984552435457857 1107 58-59 0.344559134295892 1107 60-61 0.16898331547631784 1107 62-63 0.4175864852528619 1107 64-65 0.2898422708191788 1107 66-67 0.21505649251340486 1107 68-69 0.21179012778892314 1107 70-71 0.21611709146080216 1107 72-73 0.23190762461720738 1107 74-75 0.26509444038827823 1107 76-77 0.31918337198744595 1107 78-79 0.08358525769460456 1107 80-81 0.23259991081076592 1107 82-83 0.3441711902673532 1107 84-85 0.3030585443957321 1107 86-87 0.1829978136440893 1107 88-89 0.09132862812519704 1107 90-91 0.1405210530376202 1107 92-93 0.28439608154812746 1107 94-95 0.3680573527511122 1107 96-97 0.2831626231283657 1107 98-99 0.08389999489319067 1107 100-101 -0.1389077538434691 1108 1 0.19233093174920768 1108 2 0.10053702836682277 1108 3 0.09572154926042487 1108 4 0.19711669546670407 1108 5 0.20750208904225076 1108 6 0.3006159935459536 1108 7 0.2015727464204602 1108 8 0.27898232371640574 1108 9 0.36028361867432324 1108 10-11 0.3634678921948762 1108 12-13 0.30423513080986453 1108 14-15 0.3791014467710383 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-1.437643921774992 2316 78-79 -1.2631162454303997 2316 80-81 -1.2899342192361267 2316 82-83 -1.054785395562135 2316 84-85 -0.9420685009865082 2316 86-87 -0.9889238626159447 2316 88-89 -1.189097253999929 2316 90-91 -1.4573440036037333 2316 92-93 -1.4128633384238505 2316 94-95 -1.2291893221751344 2316 96-97 -1.2315254986866044 2316 98-99 -1.6114496441947814 2316 100-101 -1.4827215655210928 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8098.0 3 2394.0 4 2445.0 5 2610.0 6 2863.0 7 3644.0 8 4173.0 9 5136.0 10 5971.0 11 7213.0 12 9302.0 13 13350.0 14 11488.0 15 11498.0 16 13305.0 17 17109.0 18 15116.0 19 12247.0 20 12341.0 21 12680.0 22 13804.0 23 15308.0 24 16975.0 25 18923.0 26 21818.0 27 25050.0 28 29008.0 29 34395.0 30 41124.0 31 50133.0 32 62614.0 33 80840.0 34 112584.0 35 167420.0 36 269573.0 37 424981.0 38 400787.0 39 72812.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1352759318616 18.654679263654494 14.680697002572613 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22.059968823366656 20-21 23.003166630080297 26.051168189127615 28.859646075564026 22.086019105228054 22-23 22.575353145296354 25.936778435745406 28.810924940192788 22.676943478765452 24-25 22.61820359369975 26.11012744166676 28.832817676971167 22.43885128766232 26-27 22.298383936857764 26.304410720261735 28.793021785234973 22.604183557645534 28-29 22.255228781040266 26.848394673784583 28.65455299947069 22.24182354570446 30-31 22.52984157998677 26.334336573845356 28.450085807247234 22.68573603892064 32-33 22.239089099904177 26.34890191862718 28.582220525952557 22.82978845551608 34-35 22.85486059687528 26.474145004542294 28.357404921877045 22.313589476705385 36-37 22.846430917979603 26.146599074212702 28.452900787822472 22.554069219985227 38-39 22.531133996743407 26.450356946420055 28.482476062334054 22.536032994502484 40-41 22.846601201116968 26.382225705011237 28.417653683630668 22.353519410241134 42-43 22.51021680130304 26.421859315567715 28.13149258949428 22.936431293634964 44-45 22.630968527070667 26.552112122604594 28.280155072021586 22.536764278303156 46-47 23.008307153871886 26.74026609022363 27.717863597582582 22.5335631583219 48-49 22.85436424713721 26.791039513000875 27.636854054923504 22.717742184938412 50-51 22.69774448890952 26.988308203361242 27.805110657331973 22.508836650397264 52-53 22.955334266725462 26.903196942274693 27.5514739033756 22.58999488762424 54-55 22.694663600068786 26.830710682821156 27.634344081684663 22.84028163542539 56-57 22.74052824304137 27.05560397083935 27.500469379511205 22.703398406608073 58-59 22.79865385690378 26.782395463915282 27.590640318301602 22.828310360879335 60-61 22.58301287637469 26.862562592629676 27.93294445865951 22.621480072336126 62-63 22.547170628220115 26.899152023453883 27.990079107367066 22.56359824095894 64-65 22.384093209972626 27.00378451454748 27.74418136765669 22.867940907823204 66-67 22.403828995997312 26.97116744630726 27.590491065915245 23.034512491780177 68-69 22.63827897604114 26.71608456207687 27.836564011537718 22.809072450344274 70-71 22.64442452213266 26.73609168795719 27.521312390814373 23.098171399095772 72-73 22.556490530072885 26.73920150629864 27.6916512833509 23.012656680277573 74-75 22.403225538998488 27.22381347074857 27.390599635738422 22.982361354514524 76-77 22.60379234330968 26.846938197319336 27.473197179533432 23.076072279837554 78-79 22.60636218901731 26.92615574291999 27.504212731304428 22.96326933675827 80-81 22.726450720706346 26.894140959845036 27.340999922072427 23.03840839737619 82-83 22.758877668521187 26.865583381869516 27.508760018784372 22.866778930824925 84-85 22.59326215086767 27.030294813371786 27.349252692969888 23.027190342790654 86-87 22.639441442511334 26.96779189675989 27.366422223541754 23.026344437187024 88-89 22.587353030107334 26.936310860839384 27.155389345570452 23.320946763482826 90-91 22.513540950517065 26.919800648698523 27.231825329533997 23.33483307125042 92-93 22.71874085810894 26.739786945367193 27.400106819361923 23.14136537716194 94-95 22.520158062074035 26.526799500382488 27.560335596065126 23.39270684147835 96-97 22.546892931096824 26.33787559944078 27.656527763787974 23.458703705674424 98-99 22.332560183892753 26.56236078855303 27.33897504736901 23.7661039801852 100-101 22.764525766882553 25.749537612658592 27.77986032500108 23.706076295457777 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1748.0 1 981.5 2 266.5 3 311.0 4 275.0 5 281.0 6 464.0 7 914.5 8 1450.0 9 1770.0 10 1759.0 11 1682.5 12 1746.0 13 1905.5 14 2136.0 15 2363.0 16 2548.5 17 2688.0 18 2849.0 19 3050.5 20 3384.5 21 3985.0 22 4707.0 23 5694.0 24 7146.5 25 8895.0 26 11381.5 27 14547.5 28 17201.5 29 19857.5 30 23005.0 31 26891.0 32 32262.5 33 35815.5 34 37617.5 35 40532.5 36 44187.0 37 47951.5 38 51027.0 39 53416.5 40 56206.5 41 59701.5 42 61758.5 43 63280.5 44 65891.0 45 69366.0 46 71733.0 47 74635.0 48 79327.0 49 79415.5 50 75207.0 51 72730.5 52 73356.5 53 74417.5 54 73535.0 55 68691.5 56 62671.5 57 57221.0 58 52759.5 59 47112.5 60 38660.0 61 32031.0 62 26547.5 63 19807.5 64 13724.0 65 9999.5 66 7459.0 67 5742.5 68 4416.5 69 3547.5 70 2786.0 71 1865.5 72 1406.0 73 1167.0 74 898.0 75 651.5 76 482.0 77 326.5 78 230.5 79 158.0 80 97.5 81 64.0 82 47.0 83 39.5 84 31.5 85 27.5 86 24.5 87 15.0 88 9.5 89 11.5 90 14.0 91 10.0 92 3.5 93 4.5 94 4.5 95 2.5 96 2.0 97 1.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3900240109454858 2 0.11618993509610533 3 0.11717459556302148 4 0.12918745325939845 5 0.13105830814653915 6 0.0731110396685239 7 0.08216991596415246 8 0.07828050711983367 9 0.0791667015400582 10-11 0.07653273479105752 12-13 0.0805206096820679 14-15 0.07471111292726264 16-17 0.07670505037276784 18-19 0.08628087341352737 20-21 0.08431155247969506 22-23 0.09494588552238946 24-25 0.10779570461564518 26-27 0.10900191368761744 28-29 0.1033401160028496 30-31 0.09351812784536104 32-33 0.09194267109829521 34-35 0.3371477438720887 36-37 0.5275072286386527 38-39 0.5088479127905917 40-41 0.4819174490204352 42-43 0.5092417769773583 44-45 0.5192853137399029 46-47 0.5164051818741732 48-49 0.5228300914208011 50-51 0.5216238823488287 52-53 0.5203930567651835 54-55 0.5149528076854718 56-57 0.48600378995813714 58-59 0.4764771999407234 60-61 0.4260872005462896 62-63 0.5006752309151877 64-65 0.5261287039849701 66-67 0.5360737747008232 68-69 0.5501051863543783 70-71 0.5458957628583119 72-73 0.5337105895802244 74-75 0.536910736097702 76-77 0.5133773509384061 78-79 0.4875546301935301 80-81 0.4947672681136908 82-83 0.5084786651154982 84-85 0.5022014546389078 86-87 0.5039984599910298 88-89 0.5130081032633125 90-91 0.5319135842281025 92-93 0.5378215470295994 94-95 0.532282831903196 96-97 0.5335136574868412 98-99 0.5341290702786639 100-101 0.5117526611679944 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2031157.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.557024849956385 #Duplication Level Percentage of deduplicated Percentage of total 1 79.8922212875604 49.1792745111657 2 12.049446696162317 14.834561794077771 3 3.5830733451997707 6.6169000484903595 4 1.5088428954899333 3.7151951844941586 5 0.7963968286300326 2.451190968520269 6 0.4804809345675177 1.7746186097461771 7 0.2993524481589729 1.2899072263152023 8 0.2277814185799539 1.121723715510763 9 0.16200334891746532 0.897519975757971 >10 0.9113649226486475 10.268460628991297 >50 0.059694780138301234 2.4933636152878944 >100 0.025895682768698986 3.0210958446285363 >500 0.0024037752404879576 1.0276464566153054 >1k 9.615100961951797E-4 1.0513708600533616 >5k 8.01258413495983E-5 0.25717056034519736 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5214 0.25670098372503947 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4391 0.21618220551144005 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2760 0.13588314443442825 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2187 0.10767262205728066 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.923302334580734E-5 2 0.0 0.0 0.0 0.0 4.923302334580734E-5 3 0.0 0.0 0.0 0.0 4.923302334580734E-5 4 0.0 0.0 0.0 0.0 4.923302334580734E-5 5 0.0 0.0 0.0 0.0 4.923302334580734E-5 6 0.0 0.0 0.0 0.0 4.923302334580734E-5 7 0.0 0.0 0.0 0.0 4.923302334580734E-5 8 0.0 0.0 0.0 0.0 4.923302334580734E-5 9 0.0 0.0 0.0 0.0 4.923302334580734E-5 10-11 0.0 0.0 0.0 0.0 4.923302334580734E-5 12-13 0.0 0.0 0.0 4.923302334580734E-5 2.953981400748441E-4 14-15 0.0 0.0 0.0 4.923302334580734E-5 3.446311634206514E-4 16-17 0.0 0.0 0.0 4.923302334580734E-5 4.4309721011226606E-4 18-19 0.0 0.0 0.0 4.923302334580734E-5 4.4309721011226606E-4 20-21 0.0 0.0 0.0 4.923302334580734E-5 5.90796280149688E-4 22-23 0.0 0.0 0.0 4.923302334580734E-5 6.400293034954954E-4 24-25 0.0 0.0 0.0 2.461651167290367E-4 6.892623268413028E-4 26-27 0.0 0.0 0.0 6.892623268413028E-4 7.631118618600138E-4 28-29 4.923302334580734E-5 0.0 0.0 0.0018954713988135826 8.369613968787248E-4 30-31 4.923302334580734E-5 0.0 0.0 0.005981812336515592 8.369613968787248E-4 32-33 4.923302334580734E-5 0.0 0.0 0.013957562118536382 8.615779085516284E-4 34-35 4.923302334580734E-5 0.0 0.0 0.025330390511417875 9.354274435703395E-4 36-37 4.923302334580734E-5 0.0 0.0 0.045220531943124045 9.354274435703395E-4 38-39 4.923302334580734E-5 0.0 0.0 0.07601578804592654 9.600439552432432E-4 40-41 4.923302334580734E-5 0.0 0.0 0.10681104414872902 9.846604669161469E-4 42-43 4.923302334580734E-5 0.0 0.0 0.14307116584291613 0.0010338934902619542 44-45 4.923302334580734E-5 0.0 0.0 0.19013793616150795 0.001058510001934858 46-47 4.923302334580734E-5 0.0 0.0 0.24611588370569087 0.0011077430252806652 48-49 4.923302334580734E-5 0.0 0.0 0.3078048619579875 0.0012062090719722799 50-51 4.923302334580734E-5 0.0 0.0 0.37559873510516417 0.0012308255836451835 52-53 4.923302334580734E-5 0.0 0.0 0.44445111825427575 0.0013785246536826056 54-55 4.923302334580734E-5 0.0 0.0 0.5221408290939598 0.0015262237237200276 56-57 4.923302334580734E-5 0.0 0.0 0.6159543550793957 0.0015262237237200276 58-59 4.923302334580734E-5 0.0 0.0 0.719072922477189 0.0015262237237200276 60-61 4.923302334580734E-5 0.0 0.0 0.8327027403593124 0.0015262237237200276 62-63 4.923302334580734E-5 0.0 0.0 0.9487942094087262 0.0015754567470658348 64-65 4.923302334580734E-5 0.0 0.0 1.0769970022012085 0.0015754567470658348 66-67 4.923302334580734E-5 0.0 0.0 1.227896218756108 0.0016000732587387384 68-69 4.923302334580734E-5 0.0 0.0 1.3958546779003296 0.0016246897704116422 70-71 4.923302334580734E-5 0.0 0.0 1.5862880122019125 0.001649306282084546 72-73 4.923302334580734E-5 0.0 0.0 1.7848940283788992 0.0017723888404490643 74-75 9.846604669161467E-5 0.0 0.0 1.9927558529448979 0.001870854887140679 76-77 9.846604669161467E-5 0.0 0.0 2.225800368952277 0.002018553957178101 78-79 9.846604669161467E-5 0.0 0.0 2.4896893740858044 0.002018553957178101 80-81 9.846604669161467E-5 0.0 0.0 2.777333312983684 0.002067786980523908 82-83 9.846604669161467E-5 0.0 0.0 3.0825534412160165 0.002313952097252945 84-85 9.846604669161467E-5 0.0 0.0 3.398604834584426 0.002313952097252945 86-87 9.846604669161467E-5 0.0 0.0 3.734497136361197 0.0024124181439445598 88-89 9.846604669161467E-5 0.0 0.0 4.095793678184404 0.0025108841906361745 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8700 0.0 28.875835 1 GGTATCA 5990 0.0 26.55927 1 TCAACGC 10055 0.0 24.351595 4 ATCAACG 10060 0.0 24.339495 3 CAACGCA 10115 0.0 24.207148 5 TATCAAC 10220 0.0 24.19015 2 AACGCAG 10335 0.0 23.679594 6 GTACATG 6360 0.0 22.102058 1 TAAGGTG 1055 0.0 21.996956 5 TACATGG 6270 0.0 21.603168 2 ACGCAGA 11440 0.0 21.309605 7 CGCAGAG 11470 0.0 21.130062 8 AGAGTAC 8625 0.0 20.7459 10-11 GAGTACT 6010 0.0 20.715826 12-13 GTGTAAG 940 0.0 20.71353 1 ACATGGG 6250 0.0 20.459864 3 GTAAGGT 955 0.0 20.332905 4 GTACTTT 6315 0.0 19.566095 14-15 AGTACTT 6435 0.0 19.494778 12-13 GCAGAGT 12600 0.0 18.82181 9 >>END_MODULE