FastQCFastQC Report
Fri 27 May 2016
SRR939162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939162_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031157
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52270.25734101302853496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47410.23341376368247263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28300.13932945606863478No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25530.12569190860184615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87400.029.3375111
GGTATCA62150.028.6802461
ATCAACG102150.024.6215293
TCAACGC102750.024.4777534
TATCAAC104700.024.112512
CAACGCA104200.024.0915895
AACGCAG106750.023.6050076
TAAGGTG10000.021.8290755
ACGCAGA117100.021.397087
GAGTACT67350.021.38552912-13
CGCAGAG118100.021.175728
TACATGG59600.021.0201192
GTACATG62950.020.8204151
AGAGTAC92600.020.67908910-11
GTACTTT71300.020.13421414-15
ACATGGG58400.019.9893993
TAGCCCG1200.00479121719.772715
AGTACTT71750.019.34651812-13
GTGTAGC13200.018.7750821
GCAGAGT132600.018.6454019
Produced by FastQC (version 0.11.5)