##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939162_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2031157 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48283712189654 34.0 31.0 34.0 31.0 34.0 2 32.70526305942869 34.0 31.0 34.0 31.0 34.0 3 32.81496506670828 34.0 31.0 34.0 31.0 34.0 4 36.26839382676967 37.0 37.0 37.0 35.0 37.0 5 36.22545869177026 37.0 37.0 37.0 35.0 37.0 6 36.259192174706335 37.0 37.0 37.0 35.0 37.0 7 36.217480480337066 37.0 37.0 37.0 35.0 37.0 8 36.18373419681492 37.0 37.0 37.0 35.0 37.0 9 37.93596260653411 39.0 38.0 39.0 35.0 39.0 10-11 37.851383964902766 39.0 38.0 39.0 35.0 39.0 12-13 37.76396703947553 39.0 38.0 39.0 35.0 39.0 14-15 39.182138800693394 40.5 38.5 41.0 36.0 41.0 16-17 39.27180862926893 41.0 39.0 41.0 36.0 41.0 18-19 39.29537056958177 41.0 39.0 41.0 36.0 41.0 20-21 39.25595928822834 41.0 39.0 41.0 36.0 41.0 22-23 39.1851082904965 41.0 39.0 41.0 36.0 41.0 24-25 39.11425950825072 41.0 39.0 41.0 35.5 41.0 26-27 38.964006967457465 40.0 38.5 41.0 35.0 41.0 28-29 38.80963583809622 40.0 38.0 41.0 35.0 41.0 30-31 38.610902062223644 40.0 38.0 41.0 34.5 41.0 32-33 38.51860146704563 40.0 38.0 41.0 34.0 41.0 34-35 38.41192753686692 40.0 38.0 41.0 34.0 41.0 36-37 38.26329722419291 40.0 38.0 41.0 34.0 41.0 38-39 38.12622140976793 40.0 38.0 41.0 33.5 41.0 40-41 37.93635745538134 40.0 38.0 41.0 33.0 41.0 42-43 37.74709709786097 40.0 37.0 41.0 33.0 41.0 44-45 37.4974470215744 40.0 37.0 41.0 32.5 41.0 46-47 37.19945011636226 40.0 37.0 41.0 31.5 41.0 48-49 36.85216455448791 40.0 36.0 41.0 31.0 41.0 50-51 36.57938800398 39.0 36.0 41.0 30.5 41.0 52-53 36.293945273555906 39.0 35.5 41.0 30.0 41.0 54-55 36.02653561492292 39.0 35.0 40.0 29.5 41.0 56-57 35.74550120940922 38.5 35.0 40.0 28.5 41.0 58-59 35.375881578824284 38.0 35.0 40.0 28.0 41.0 60-61 34.9676391337548 38.0 34.0 40.0 27.5 41.0 62-63 34.54603755396555 37.0 34.0 40.0 26.0 41.0 64-65 34.06475594944162 37.0 33.5 39.5 26.0 41.0 66-67 33.61316924294872 36.0 33.0 39.0 25.5 40.5 68-69 33.098755536868886 35.5 32.5 39.0 23.5 40.0 70-71 32.558551850004704 35.0 32.0 38.0 22.5 40.0 72-73 31.960505022506876 35.0 31.0 37.0 20.5 39.5 74-75 31.396154507012504 35.0 31.0 37.0 18.5 39.0 76-77 29.423860883230592 32.5 28.5 35.0 15.5 37.0 78-79 30.399360561492784 34.0 30.0 35.5 17.0 37.5 80-81 30.409876981444565 34.0 30.0 35.0 14.5 37.0 82-83 30.004291396479935 34.0 30.0 35.0 8.5 37.0 84-85 29.504702246059757 34.0 29.5 35.0 4.5 36.0 86-87 28.768897726763612 34.0 29.0 35.0 2.0 36.0 88-89 28.053413891688336 33.0 27.5 35.0 2.0 35.0 90-91 27.453752713355 33.0 26.0 35.0 2.0 35.0 92-93 26.96064312113736 32.5 25.5 34.0 2.0 35.0 94-95 26.36492378481821 32.0 25.0 34.0 2.0 35.0 96-97 25.502945365621663 32.0 22.5 34.0 2.0 35.0 98-99 24.231862677281963 31.5 10.0 34.0 2.0 35.0 100-101 18.39679035150902 24.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06587210904180552 1101 2 0.1469716416199418 1101 3 0.14784732181476556 1101 4 0.09306018498998014 1101 5 0.11830989280608861 1101 6 0.11275068078757045 1101 7 0.15010294189556816 1101 8 0.14692257304707823 1101 9 0.1892352792737384 1101 10-11 0.1579104705883836 1101 12-13 0.1338673989232646 1101 14-15 0.2521630464155251 1101 16-17 0.16321282399179182 1101 18-19 0.21160977453472896 1101 20-21 0.2256302067868532 1101 22-23 0.174126606054962 1101 24-25 0.20384600165221656 1101 26-27 0.23855389969875773 1101 28-29 0.19315400175169373 1101 30-31 0.1443277072055693 1101 32-33 0.24717120616393373 1101 34-35 0.12585871187165054 1101 36-37 0.12902093846376772 1101 38-39 0.08751657122710554 1101 40-41 0.23191745808795616 1101 42-43 0.22876560544909097 1101 44-45 0.3536943643932986 1101 46-47 0.32386147543373056 1101 48-49 0.25798336931251953 1101 50-51 0.16643427296013868 1101 52-53 0.14151479757208563 1101 54-55 0.2962047354404831 1101 56-57 0.23628830317679927 1101 58-59 0.2532194546546904 1101 60-61 0.21276601532122186 1101 62-63 -0.12773558349514502 1101 64-65 -0.025085425708475384 1101 66-67 5.491703408750936E-4 1101 68-69 0.1152754743879072 1101 70-71 0.06483359998028249 1101 72-73 0.23178341789702728 1101 74-75 -0.09747737465836437 1101 76-77 -0.12017515389034372 1101 78-79 -0.17682518539703906 1101 80-81 0.01898255347770217 1101 82-83 -0.061285642496326176 1101 84-85 -0.19144533034369005 1101 86-87 -0.054751605133450454 1101 88-89 0.05617062527624128 1101 90-91 0.17180702834714978 1101 92-93 0.022736563406493104 1101 94-95 -0.4419235237674499 1101 96-97 -0.5226065000708395 1101 98-99 -0.932631435957429 1101 100-101 -1.268405325115335 1102 1 0.1582818869599123 1102 2 0.14992978500482934 1102 3 0.11917563892721716 1102 4 0.13601089024067647 1102 5 0.10354112870825816 1102 6 0.14039413201727058 1102 7 0.16173309746040587 1102 8 0.19077915523087796 1102 9 0.14701095431508548 1102 10-11 0.25628108660084337 1102 12-13 0.26585311925522603 1102 14-15 0.3109131686895452 1102 16-17 0.3603220886980054 1102 18-19 0.3028497573469622 1102 20-21 0.3808696201169326 1102 22-23 0.26993439097923755 1102 24-25 0.287937433883684 1102 26-27 0.33854482801600483 1102 28-29 0.32837292147247155 1102 30-31 0.30263292962160193 1102 32-33 0.3556614295694587 1102 34-35 0.396033924483163 1102 36-37 0.3630332275269694 1102 38-39 0.31149415868249264 1102 40-41 0.4891606802334536 1102 42-43 0.40139924408813243 1102 44-45 0.41663344110261136 1102 46-47 0.4435444120504357 1102 48-49 0.44895084652274164 1102 50-51 0.18278541616972888 1102 52-53 0.21299336241250444 1102 54-55 0.21667852263559695 1102 56-57 0.24475418477164368 1102 58-59 -0.003784546247956655 1102 60-61 0.12564912846448095 1102 62-63 -0.028728012675486525 1102 64-65 0.180644681721553 1102 66-67 0.09713192232785417 1102 68-69 0.14040581122497997 1102 70-71 0.12938524093793546 1102 72-73 0.18325104506442358 1102 74-75 -0.05765914406264372 1102 76-77 0.03919542628734973 1102 78-79 0.13170791058749742 1102 80-81 0.22929127801524984 1102 82-83 0.23355147237280605 1102 84-85 0.17118647136030418 1102 86-87 0.3312226693821252 1102 88-89 0.04820223347252295 1102 90-91 0.24178876553243 1102 92-93 0.11330165821849292 1102 94-95 0.19378723611995952 1102 96-97 0.03375641372783278 1102 98-99 0.12871202537702686 1102 100-101 -0.46534881369712267 1103 1 0.09897954397159481 1103 2 0.12693275638068968 1103 3 0.13804733148393922 1103 4 0.08855264445766409 1103 5 0.13128554260763536 1103 6 0.07123296202966145 1103 7 0.10195330861314034 1103 8 0.11545036575520129 1103 9 0.22363193715283103 1103 10-11 0.1672568629918274 1103 12-13 0.1787783335245834 1103 14-15 0.15857067406518865 1103 16-17 0.245178964826124 1103 18-19 0.30722082355459435 1103 20-21 0.341079173939157 1103 22-23 0.35735125599774875 1103 24-25 0.3437560783515039 1103 26-27 0.2856617270831876 1103 28-29 0.2911866096127298 1103 30-31 0.2730978548690004 1103 32-33 0.27694704252362357 1103 34-35 0.34226568543025593 1103 36-37 0.3531030543819753 1103 38-39 0.3986681601470039 1103 40-41 0.4393140903721786 1103 42-43 0.34868893460193107 1103 44-45 0.2905224398773072 1103 46-47 0.26306939960466735 1103 48-49 0.18825441895803152 1103 50-51 0.17277848221619507 1103 52-53 0.17269266388914417 1103 54-55 0.13611168387820527 1103 56-57 0.0036897321287696627 1103 58-59 -0.012686570471885261 1103 60-61 0.09376552333099397 1103 62-63 0.03181428810393783 1103 64-65 0.18068640647640422 1103 66-67 0.020672423846662014 1103 68-69 -0.1290526556036795 1103 70-71 -0.11087895638766554 1103 72-73 0.021360429535107528 1103 74-75 -0.15483689438489634 1103 76-77 -0.173139940092625 1103 78-79 -0.1933604176799406 1103 80-81 0.022647359302713 1103 82-83 -0.008936081069119695 1103 84-85 -0.16710318953210646 1103 86-87 -0.2092426985935809 1103 88-89 0.03671805209151202 1103 90-91 0.15444754653893966 1103 92-93 -0.0324303293481627 1103 94-95 0.11009757019509792 1103 96-97 -0.18418026210872185 1103 98-99 -0.24677525446517024 1103 100-101 -0.7306448380924095 1104 1 0.05204536569102913 1104 2 0.03409582954618884 1104 3 0.0816845102623418 1104 4 0.03466800505924539 1104 5 0.02750445875377494 1104 6 0.019485445024827186 1104 7 0.058647012491043427 1104 8 0.012321145488563445 1104 9 0.06745697366544334 1104 10-11 0.13196546346546967 1104 12-13 0.14062093459781977 1104 14-15 0.08724249513000615 1104 16-17 0.07016596881157255 1104 18-19 0.14981364535379527 1104 20-21 0.0860148539814034 1104 22-23 0.12877267618021904 1104 24-25 0.23364751693467412 1104 26-27 0.14053818930672435 1104 28-29 0.09583106890976723 1104 30-31 0.17597430609062314 1104 32-33 0.09614291240395545 1104 34-35 0.19494038899735244 1104 36-37 0.20309191494092715 1104 38-39 0.23553585934358523 1104 40-41 0.29916892007626217 1104 42-43 0.18030184315124842 1104 44-45 0.11239590132796451 1104 46-47 0.15038226387127196 1104 48-49 0.08600122633921359 1104 50-51 0.1216374458906131 1104 52-53 0.04318359693423446 1104 54-55 0.05547313261064346 1104 56-57 0.13149350193565823 1104 58-59 0.13630964211815666 1104 60-61 0.10818216043001172 1104 62-63 0.08457123100124164 1104 64-65 0.19417750730071504 1104 66-67 0.1880707179994232 1104 68-69 0.040437378034681615 1104 70-71 -0.04751066446280561 1104 72-73 -0.13092535626306656 1104 74-75 -0.21191484021695928 1104 76-77 -0.2287683833488643 1104 78-79 0.04072462163815871 1104 80-81 0.1390751468446929 1104 82-83 0.011317073042985015 1104 84-85 -0.09881153971212342 1104 86-87 -0.163938179281093 1104 88-89 -0.0816777033688787 1104 90-91 0.013363087571967469 1104 92-93 -0.2081116918255752 1104 94-95 -0.21290083727739884 1104 96-97 -0.7032012015074152 1104 98-99 -1.2014212265258024 1104 100-101 -1.33692060664508 1105 1 -0.05676512921512966 1105 2 0.012053701608621736 1105 3 0.04501766703264565 1105 4 0.08437314480887892 1105 5 -0.011231703119207737 1105 6 0.02551242385492003 1105 7 0.056130106581477435 1105 8 0.03863168453548127 1105 9 0.08108587350123742 1105 10-11 0.03723984240502176 1105 12-13 -0.0034981924745878246 1105 14-15 -0.02746343915323024 1105 16-17 0.1261726428938843 1105 18-19 0.17938000656642572 1105 20-21 0.12488034826959193 1105 22-23 0.05412094674539247 1105 24-25 0.07071074415363654 1105 26-27 0.08919057202474079 1105 28-29 0.03961693327404703 1105 30-31 0.03457954648117578 1105 32-33 0.11556396224769827 1105 34-35 0.18987965131703533 1105 36-37 0.2971165807954037 1105 38-39 0.26782217394357843 1105 40-41 0.13725135735224825 1105 42-43 0.09766769939363229 1105 44-45 0.10803788448385632 1105 46-47 0.19948399948579976 1105 48-49 0.23026690072169487 1105 50-51 0.057478415574742314 1105 52-53 0.04732973814933672 1105 54-55 0.0605510851858142 1105 56-57 0.1380285408678006 1105 58-59 0.05675652313234281 1105 60-61 -0.1379413983743092 1105 62-63 -0.2672941573491343 1105 64-65 -0.29960438439552206 1105 66-67 -0.3232906711984498 1105 68-69 -0.37967359182601257 1105 70-71 -0.34986645661281557 1105 72-73 -0.4207632714068765 1105 74-75 -0.46220572779928304 1105 76-77 -0.5077529693968543 1105 78-79 -0.3470572170891728 1105 80-81 -0.18575711828234276 1105 82-83 -0.21666198658234848 1105 84-85 -0.45838081421466015 1105 86-87 -0.540773110509793 1105 88-89 -0.37706367013971587 1105 90-91 -0.2960905381497376 1105 92-93 -0.4071304428187439 1105 94-95 -0.4738278814358132 1105 96-97 -0.6784355889219995 1105 98-99 -0.6893235695171356 1105 100-101 -1.253351561674478 1106 1 0.10260865497554761 1106 2 0.08149903882127063 1106 3 0.11618219387489859 1106 4 0.08575768732626443 1106 5 0.0694878868464528 1106 6 0.12397789627965494 1106 7 0.13233858581192948 1106 8 0.11084187788087974 1106 9 0.1342758752491875 1106 10-11 0.15033999408164078 1106 12-13 0.12772703320781886 1106 14-15 0.14760633772988996 1106 16-17 0.21259645781457692 1106 18-19 0.17703352980564802 1106 20-21 0.21102773396237495 1106 22-23 0.25172992555013707 1106 24-25 0.3136400199964555 1106 26-27 0.2860940290111671 1106 28-29 0.25430362802062945 1106 30-31 0.25912028772312823 1106 32-33 0.23650858618552917 1106 34-35 0.205475137105104 1106 36-37 0.136501050011816 1106 38-39 0.2737634993378535 1106 40-41 0.2626077128281139 1106 42-43 0.2682162161767323 1106 44-45 0.25494134147027836 1106 46-47 0.2614378367914796 1106 48-49 0.15983975342621903 1106 50-51 0.10501028739954421 1106 52-53 0.11292578779100637 1106 54-55 0.008629754040235582 1106 56-57 -0.03660467236471021 1106 58-59 -0.1713845643157157 1106 60-61 -0.236547552272512 1106 62-63 -0.19569715479487826 1106 64-65 -0.13334383328501076 1106 66-67 0.024358482175365737 1106 68-69 -0.26361010754907 1106 70-71 -0.2910081755769056 1106 72-73 -0.4649798250208974 1106 74-75 -0.5547472133279783 1106 76-77 -0.6127112160376953 1106 78-79 -0.27571620406979136 1106 80-81 -0.007085585559099883 1106 82-83 -0.13233683476476799 1106 84-85 -0.355543643857569 1106 86-87 -0.38518109242136944 1106 88-89 -0.5082311008280165 1106 90-91 -0.3556076821184746 1106 92-93 -0.3745398456413973 1106 94-95 -0.5491650658892411 1106 96-97 -0.6406148267047023 1106 98-99 -0.5539480994041952 1106 100-101 -1.0434869429240905 1107 1 0.037395479013255795 1107 2 -0.04318261907546628 1107 3 -0.004699236148795194 1107 4 0.00898426793520457 1107 5 -0.011027074860542996 1107 6 -0.03323557283997758 1107 7 0.07109763778867517 1107 8 -4.056088533843649E-5 1107 9 0.009072808347383443 1107 10-11 0.026940358689330424 1107 12-13 0.01054144108001509 1107 14-15 -0.03263404588433616 1107 16-17 0.024349011380024876 1107 18-19 0.01230546975274649 1107 20-21 0.09739153627804598 1107 22-23 0.01297652577682129 1107 24-25 0.00304616813340175 1107 26-27 0.06070579159806755 1107 28-29 0.06467585401248499 1107 30-31 -0.016711025646124256 1107 32-33 0.009731502327660735 1107 34-35 -0.03284420589790926 1107 36-37 0.051166029851842154 1107 38-39 -0.021627547950537007 1107 40-41 -0.025282400764950808 1107 42-43 -0.19957758278818005 1107 44-45 -0.19478934050187036 1107 46-47 -0.08762899524045054 1107 48-49 -0.34765834064538126 1107 50-51 -0.35221051065371967 1107 52-53 -0.2571785994817404 1107 54-55 -0.30051575449986245 1107 56-57 -0.4662313592026237 1107 58-59 -0.3547589459876477 1107 60-61 -0.5102084465700827 1107 62-63 -0.4119530270691385 1107 64-65 -0.356991381351051 1107 66-67 -0.2817482233121069 1107 68-69 -0.5204424450759433 1107 70-71 -0.4346636583629646 1107 72-73 -0.5985677599194119 1107 74-75 -0.733623901450482 1107 76-77 -0.7041941567953032 1107 78-79 -0.5432019985140286 1107 80-81 -0.31157979042794537 1107 82-83 -0.5990115515393448 1107 84-85 -0.5835579923410599 1107 86-87 -0.5392214848519608 1107 88-89 -0.604272989875529 1107 90-91 -0.45733219913228496 1107 92-93 -0.28842622769843373 1107 94-95 -0.29173437907621036 1107 96-97 -0.3655049129832868 1107 98-99 -0.48284270420023745 1107 100-101 -1.0044764625490004 1108 1 -0.004391962431284924 1108 2 0.020107590397593356 1108 3 0.001799272469483526 1108 4 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-0.14691573812072178 2316 88-89 -0.47119603943894006 2316 90-91 -0.6160293590618622 2316 92-93 -0.5346579425097566 2316 94-95 -0.20956203049031785 2316 96-97 -0.20322856877615791 2316 98-99 0.20599530330451898 2316 100-101 1.1735610572848607 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 16.0 5 34.0 6 80.0 7 157.0 8 456.0 9 931.0 10 1631.0 11 2551.0 12 3732.0 13 5378.0 14 6879.0 15 9230.0 16 11628.0 17 16162.0 18 16678.0 19 13346.0 20 12493.0 21 13338.0 22 14454.0 23 15489.0 24 17105.0 25 20286.0 26 23424.0 27 28314.0 28 33350.0 29 40708.0 30 48928.0 31 60408.0 32 75832.0 33 97984.0 34 135986.0 35 201630.0 36 324486.0 37 473856.0 38 290421.0 39 13773.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.39910408132489 19.166874741966026 15.104931755422113 23.32908942128697 2 15.654772132336397 20.186819630388 40.23514676610424 23.923261471171354 3 19.66701736990297 23.685515201434455 29.625282536012726 27.022184892649854 4 13.425303903144858 16.83021056471755 36.25682308162293 33.487662450514655 5 14.079216919223873 36.87450059251944 34.16289336570241 14.883389122554288 6 32.770928096646394 35.65051839911932 17.110051069415118 14.46850243481917 7 28.86463232532 32.13099725919759 20.542331291968075 18.46203912351433 8 25.963379492574923 35.76788992677572 19.373440851691917 18.895289728957437 9 25.814400363930506 17.518586697138627 20.359726008378477 36.307286930552394 10-11 25.182494509287075 26.50959527008498 26.916235426409678 21.39167479421827 12-13 26.033905798517793 23.618533673172482 27.526971081014416 22.820589447295312 14-15 22.660671012998623 25.241096370606037 25.830347322535445 26.267885293859894 16-17 22.362104511690024 28.257543587565927 26.76909821587923 22.61125368486482 18-19 22.328825393605715 26.994860564692928 28.683971746152565 21.99234229554879 20-21 22.878684414843363 26.07533538766329 28.995050604163048 22.050929593330302 22-23 22.482137028304557 26.026299296410865 28.892448983510384 22.599114691774197 24-25 22.567356996388753 26.181507565892314 28.954658802503996 22.29647663521494 26-27 22.225652251190375 26.369406242291664 28.910417654441154 22.4945238520768 28-29 22.240222375884073 26.850012222107072 28.728031901053363 22.18173350095549 30-31 22.44892935405781 26.345230821645004 28.636683427228917 22.569156397068273 32-33 22.236740497347203 26.39949211200614 28.678563173246374 22.685204217400283 34-35 22.81082494379345 26.496871833303626 28.468458842425683 22.223844380477246 36-37 22.756885516272753 26.175525201134835 28.59086685088124 22.47672243171117 38-39 22.405032467332617 26.547082618177125 28.60887457660003 22.439010337890224 40-41 22.769664966911268 26.405030875511482 28.530139536189083 22.295164621388164 42-43 22.463076113686647 26.453339872085657 28.223382095783368 22.86020191844433 44-45 22.603382139855572 26.559846702322552 28.404283236365263 22.432487921456605 46-47 23.001815574552413 26.769175852243006 27.802416252777213 22.426592320427368 48-49 22.83572437354435 26.850343128650906 27.72878792391965 22.5851445738851 50-51 22.739096581452635 27.031020256845846 27.81498867262014 22.41489448908138 52-53 22.99923147250557 26.962317536261356 27.543341061276898 22.49510992995618 54-55 22.66156678188835 26.917737033621727 27.645425735184432 22.77527044930549 56-57 22.74565678576299 27.10753033861981 27.51498283982971 22.631830035787484 58-59 22.789717922542 26.836831679366107 27.578981417454795 22.7944689806371 60-61 22.574974490518105 26.960217516798103 27.864797899668037 22.600010093015758 62-63 22.549777180298715 26.95491885906663 27.956776695099983 22.538527265534665 64-65 22.37610971189771 27.065560239111637 27.73546767603687 22.82286237295379 66-67 22.394256183831263 27.011626816390045 27.59078903867736 23.00332796110133 68-69 22.611876255809154 26.776916104439998 27.78001251562607 22.831195124124783 70-71 22.618953255659957 26.842953885843272 27.495021305611832 23.043071552884935 72-73 22.594659103161398 26.81919713739509 27.577434929943866 23.008708829499643 74-75 22.45072635940993 27.303576237582817 27.2303667318676 23.01533067113965 76-77 22.535439624815908 26.941950497008538 27.412433677370363 23.1101762008052 78-79 22.628573060549588 26.995387867923586 27.42282194136957 22.95321713015726 80-81 22.714141988281003 26.946529952418498 27.283605589257103 23.055722470043396 82-83 22.73573633155881 26.977038210241748 27.3698192704946 22.917406187704838 84-85 22.55118634354705 27.139581036817933 27.268965422170716 23.040267197464303 86-87 22.626577404546218 27.047979909369392 27.279595841410973 23.045846844673413 88-89 22.541504040833267 27.038839115434122 27.030632119167564 23.389024724565047 90-91 22.492283550056435 26.948517942260235 27.12259848207307 23.436600025610257 92-93 22.640495047896348 26.82197880321413 27.21771384486773 23.3198123040218 94-95 22.437217417761754 26.624413234529193 27.36893981335264 23.569429534356413 96-97 22.456615233735068 26.443235664017028 27.505906118017005 23.594242984230902 98-99 22.26641984486659 26.675906073145573 27.111421826497732 23.9462522554901 100-101 22.84812122396702 25.43323374276487 27.847876288771456 23.87076874449665 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 271.0 1 220.5 2 198.0 3 233.5 4 197.0 5 196.5 6 337.5 7 739.5 8 1201.5 9 1445.0 10 1402.5 11 1282.0 12 1328.0 13 1420.0 14 1556.0 15 1716.0 16 1870.0 17 2056.0 18 2261.5 19 2517.5 20 2957.0 21 3641.5 22 4505.0 23 5702.0 24 7241.5 25 8944.5 26 11428.5 27 14703.0 28 17497.0 29 20055.5 30 23258.0 31 27550.5 32 32939.0 33 36268.0 34 37865.5 35 40636.0 36 44557.5 37 48377.0 38 51528.0 39 54072.5 40 56750.0 41 60072.5 42 62225.0 43 63508.5 44 66125.0 45 69969.0 46 72310.5 47 75299.0 48 79847.5 49 79723.0 50 75755.0 51 73564.5 52 73895.0 53 74668.5 54 73990.5 55 69332.5 56 63443.5 57 57595.0 58 52630.5 59 46827.0 60 38449.5 61 31928.5 62 25958.0 63 19112.5 64 13336.0 65 9675.0 66 7143.0 67 5490.0 68 4185.5 69 3276.0 70 2601.0 71 1803.5 72 1270.0 73 1015.0 74 756.5 75 548.0 76 399.5 77 240.0 78 149.0 79 93.5 80 58.5 81 36.5 82 22.5 83 16.0 84 7.5 85 4.5 86 3.5 87 2.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4259641179879251 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.354274435703394E-4 16-17 0.0021908695388884267 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.846604669161469E-5 26-27 0.0037417097742813575 28-29 7.3849535018711E-5 30-31 0.0 32-33 2.4616511672903672E-5 34-35 0.012554420953180871 36-37 0.027275094933577267 38-39 0.02085018538694941 40-41 0.008788094667226609 42-43 0.009280424900684684 44-45 0.0037417097742813575 46-47 0.005883346289823977 48-49 0.0033232290758419955 50-51 8.123448852058211E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0015016072120471239 60-61 0.0024370346556174633 62-63 0.0015016072120471239 64-65 0.0027324327956923075 66-67 0.001649306282084546 68-69 0.004726370241197504 70-71 9.846604669161469E-5 72-73 0.0 74-75 0.0 76-77 0.09681674040953013 78-79 0.015631484912293832 80-81 5.169467451309771E-4 82-83 0.0 84-85 0.0 86-87 0.1059248497285045 88-89 0.4181114507642688 90-91 0.41995768913973663 92-93 0.0 94-95 2.4616511672903672E-5 96-97 2.4616511672903672E-5 98-99 9.846604669161469E-5 100-101 3.69247675093555E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2031157.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.01881002206557 #Duplication Level Percentage of deduplicated Percentage of total 1 78.72731568318702 47.25119803536384 2 12.66245527997941 15.199709957239705 3 3.8088055500330156 6.857999301552614 4 1.6460540878962637 3.9517683034996107 5 0.8504942149192323 2.5522825355051606 6 0.4908029120644708 1.7674444042484416 7 0.3321072833712837 1.395287876332376 8 0.2282860782936801 1.0961167011032618 9 0.17415904284375142 0.9407536655457504 >10 0.9806709614913438 10.729067743104137 >50 0.0669979940271604 2.716888825139881 >100 0.027994642969540393 3.0732549206557973 >500 0.002789641349079394 1.1389736878731844 >1k 9.845792996750734E-4 1.0718535730142937 >5k 8.204827497292278E-5 0.2574004698220028 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5227 0.25734101302853496 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4741 0.23341376368247263 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2830 0.13932945606863478 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2553 0.12569190860184615 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.846604669161467E-5 2 0.0 0.0 0.0 0.0 9.846604669161467E-5 3 0.0 0.0 0.0 4.923302334580734E-5 9.846604669161467E-5 4 0.0 0.0 0.0 4.923302334580734E-5 9.846604669161467E-5 5 0.0 0.0 0.0 4.923302334580734E-5 9.846604669161467E-5 6 0.0 0.0 0.0 4.923302334580734E-5 1.47699070037422E-4 7 0.0 0.0 0.0 4.923302334580734E-5 1.47699070037422E-4 8 0.0 0.0 0.0 4.923302334580734E-5 1.47699070037422E-4 9 0.0 0.0 0.0 4.923302334580734E-5 1.47699070037422E-4 10-11 0.0 0.0 0.0 4.923302334580734E-5 1.9693209338322935E-4 12-13 0.0 0.0 0.0 9.846604669161467E-5 3.69247675093555E-4 14-15 0.0 0.0 0.0 9.846604669161467E-5 4.923302334580734E-4 16-17 0.0 0.0 0.0 9.846604669161467E-5 5.415632568038808E-4 18-19 0.0 0.0 0.0 9.846604669161467E-5 5.90796280149688E-4 20-21 0.0 0.0 0.0 9.846604669161467E-5 6.892623268413028E-4 22-23 0.0 0.0 0.0 9.846604669161467E-5 7.384953501871101E-4 24-25 0.0 0.0 0.0 2.461651167290367E-4 7.384953501871101E-4 26-27 0.0 0.0 0.0 6.892623268413028E-4 7.631118618600138E-4 28-29 0.0 0.0 0.0 0.002043170468851005 8.369613968787248E-4 30-31 0.0 0.0 0.0 0.006129511406553014 8.615779085516284E-4 32-33 0.0 0.0 0.0 0.0140806446769009 9.354274435703395E-4 34-35 0.0 0.0 0.0 0.02555193911647401 9.354274435703395E-4 36-37 0.0 0.0 0.0 0.0456143961298905 9.354274435703395E-4 38-39 0.0 0.0 0.0 0.07690198246615107 9.600439552432432E-4 40-41 0.0 0.0 0.0 0.10814033577906582 9.846604669161469E-4 42-43 0.0 0.0 0.0 0.14472047212500067 0.001058510001934858 44-45 0.0 0.0 0.0 0.19240265523541508 0.0011569760486264725 46-47 0.0 0.0 0.0 0.2487498504546916 0.0012800586069909908 48-49 0.0 0.0 0.0 0.3106603773120443 0.0013785246536826056 50-51 0.0 0.0 0.0 0.37926659534442686 0.001427757677028413 52-53 0.0 0.0 0.0 0.44895593989041715 0.0015262237237200276 54-55 0.0 0.0 0.0 0.5276056946853444 0.0015508402353929312 56-57 0.0 0.0 0.0 0.622256182067659 0.0015754567470658348 58-59 0.0 0.0 0.0 0.7267040410957892 0.0015754567470658348 60-61 0.0 0.0 0.0 0.8413431359565017 0.0016000732587387384 62-63 0.0 0.0 0.0 0.9590346782646542 0.0016246897704116422 64-65 0.0 0.0 0.0 1.08836983059409 0.0016246897704116422 66-67 0.0 0.0 0.0 1.2403029406392516 0.0016246897704116422 68-69 0.0 0.0 0.0 1.409787623507193 0.0016739227937574495 70-71 0.0 0.0 0.0 1.6023133613009728 0.0016739227937574495 72-73 0.0 0.0 0.0 1.8026917663184086 0.0018462383754677752 74-75 0.0 0.0 0.0 2.0128429264699874 0.002018553957178101 76-77 0.0 0.0 0.0 2.2489152734131332 0.002018553957178101 78-79 0.0 0.0 0.0 2.5140104876186333 0.0020677869805239084 80-81 0.0 0.0 0.0 2.8023436888433535 0.002092403492196812 82-83 0.0 0.0 0.0 3.109188506846098 0.0025108841906361745 84-85 0.0 0.0 0.0 3.4269630560316116 0.0025108841906361745 86-87 0.0 2.461651167290367E-5 0.0 3.764726212695523 0.0025847337256548857 88-89 0.0 4.923302334580734E-5 0.0 4.1261704535887676 0.0026585832606735964 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8740 0.0 29.337511 1 GGTATCA 6215 0.0 28.680246 1 ATCAACG 10215 0.0 24.621529 3 TCAACGC 10275 0.0 24.477753 4 TATCAAC 10470 0.0 24.11251 2 CAACGCA 10420 0.0 24.091589 5 AACGCAG 10675 0.0 23.605007 6 TAAGGTG 1000 0.0 21.829075 5 ACGCAGA 11710 0.0 21.39708 7 GAGTACT 6735 0.0 21.385529 12-13 CGCAGAG 11810 0.0 21.17572 8 TACATGG 5960 0.0 21.020119 2 GTACATG 6295 0.0 20.820415 1 AGAGTAC 9260 0.0 20.679089 10-11 GTACTTT 7130 0.0 20.134214 14-15 ACATGGG 5840 0.0 19.989399 3 TAGCCCG 120 0.004791217 19.77271 5 AGTACTT 7175 0.0 19.346518 12-13 GTGTAGC 1320 0.0 18.775082 1 GCAGAGT 13260 0.0 18.645401 9 >>END_MODULE