FastQCFastQC Report
Fri 27 May 2016
SRR939161_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939161_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626859
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49180.30230032227746845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39860.24501201394835076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23780.14617124163802764No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45400.035.6556551
GTATCAA66800.033.6135641
ATCAACG82450.026.48933
TCAACGC83050.026.2408814
TATCAAC84450.026.142462
CAACGCA83600.026.0115725
AACGCAG86900.025.1243216
GTACATG70400.023.465861
TACATGG71150.023.3718132
ACGCAGA98350.022.1030147
ACATGGG71450.022.0801583
CGCAGAG99400.021.7742398
GAGTACT47900.021.10873612-13
TACCTGG14000.020.6424852
ACGATAC1656.4530286E-520.0989913
GTACTTT50350.020.03641514-15
AGTACTT49450.019.6809212-13
GTGTAGC8700.019.643241
GCAGAGT107450.019.6134249
GTACCTG16000.019.2851621
Produced by FastQC (version 0.11.5)