##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939161_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1626859 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.094443341432786 33.0 31.0 34.0 30.0 34.0 2 32.19793479336562 34.0 31.0 34.0 30.0 34.0 3 32.25849566557397 34.0 31.0 34.0 30.0 34.0 4 35.68447173356757 37.0 35.0 37.0 35.0 37.0 5 35.73357310006583 37.0 35.0 37.0 35.0 37.0 6 35.81230088163756 37.0 36.0 37.0 35.0 37.0 7 35.73740686808138 37.0 36.0 37.0 35.0 37.0 8 35.72683619170438 37.0 36.0 37.0 35.0 37.0 9 37.41439792876949 39.0 38.0 39.0 35.0 39.0 10-11 37.32278027782371 39.0 37.5 39.0 34.5 39.0 12-13 37.23322058027155 39.0 37.0 39.0 34.0 39.0 14-15 38.56032176113603 40.0 38.0 41.0 34.0 41.0 16-17 38.49662847241217 40.0 38.0 41.0 34.0 41.0 18-19 38.43360026898459 40.0 38.0 41.0 34.0 41.0 20-21 38.333458523449174 40.0 38.0 41.0 34.0 41.0 22-23 38.25156820597237 40.0 38.0 41.0 34.0 41.0 24-25 38.29926932819623 40.0 38.0 41.0 34.0 41.0 26-27 38.22333220027058 40.0 38.0 41.0 34.0 41.0 28-29 38.10624368799017 40.0 38.0 41.0 33.0 41.0 30-31 37.945179022890116 40.0 38.0 41.0 33.0 41.0 32-33 37.759940166910596 40.0 38.0 41.0 33.0 41.0 34-35 37.46269252590421 40.0 37.5 41.0 32.0 41.0 36-37 37.218494657496436 40.0 37.0 41.0 31.0 41.0 38-39 36.99573257424276 40.0 37.0 41.0 30.5 41.0 40-41 36.853596101444566 40.0 37.0 41.0 30.5 41.0 42-43 36.67074036532975 40.0 36.0 41.0 30.0 41.0 44-45 36.42312517556838 40.0 36.0 41.0 30.0 41.0 46-47 36.081810408892224 39.5 36.0 41.0 28.5 41.0 48-49 36.07463523267843 40.0 36.0 41.0 28.5 41.0 50-51 35.096009549690535 38.5 34.5 40.0 26.5 40.5 52-53 35.25352473693172 38.5 35.0 40.0 27.5 40.5 54-55 35.76709966874819 39.0 35.0 41.0 28.0 41.0 56-57 35.662962493983805 39.0 35.0 41.0 28.0 41.0 58-59 35.4896862604565 39.0 35.0 41.0 27.0 41.0 60-61 35.209223417640985 39.0 35.0 41.0 26.5 41.0 62-63 34.85162512547184 38.0 35.0 40.5 26.0 41.0 64-65 34.48788555123707 38.0 34.0 40.0 26.0 41.0 66-67 34.108779248847014 37.0 34.0 40.0 25.0 41.0 68-69 33.71458436164413 36.5 34.0 39.5 23.5 41.0 70-71 33.31482630025097 36.0 34.0 39.0 22.5 41.0 72-73 32.86848614415878 35.5 33.0 39.0 22.0 40.0 74-75 32.41641900127792 35.0 33.0 37.5 20.5 40.0 76-77 31.9760203557899 35.0 32.0 37.0 19.5 39.0 78-79 31.554647022268064 35.0 32.0 36.5 18.0 39.0 80-81 31.139360571506195 35.0 31.5 36.0 15.5 38.5 82-83 30.72881116310633 35.0 31.0 36.0 10.0 37.0 84-85 30.346461494204476 35.0 31.0 35.0 7.0 37.0 86-87 29.968460696347993 34.0 31.0 35.0 2.0 36.5 88-89 29.591562022277284 34.0 30.0 35.0 2.0 36.0 90-91 29.232488494700526 34.0 30.0 35.0 2.0 36.0 92-93 28.846830917737798 34.0 29.5 35.0 2.0 35.0 94-95 28.413995619780202 34.0 29.0 35.0 2.0 35.0 96-97 27.883231736739326 34.0 29.0 35.0 2.0 35.0 98-99 27.21896181537552 34.0 27.0 35.0 2.0 35.0 100-101 24.720569821969818 31.5 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18234109614132876 1101 2 0.27536965392202717 1101 3 0.2384612314310317 1101 4 0.1760547114481028 1101 5 0.2068755148395809 1101 6 0.31577111723672147 1101 7 0.19605576021681514 1101 8 0.3263703715311337 1101 9 0.3186836032425546 1101 10-11 0.3820429729042374 1101 12-13 0.2931759656236679 1101 14-15 0.4051341980801837 1101 16-17 0.4997972457083506 1101 18-19 0.4019732682973256 1101 20-21 0.5246825121862457 1101 22-23 0.5857503570557512 1101 24-25 0.5453157708804071 1101 26-27 0.47320248822067157 1101 28-29 0.4965787874570964 1101 30-31 0.4467956004636804 1101 32-33 0.44523172400968036 1101 34-35 0.43644624338131877 1101 36-37 0.40284069189694804 1101 38-39 0.4214897783757081 1101 40-41 0.46476704573258587 1101 42-43 0.4273256580173168 1101 44-45 0.4986199592743361 1101 46-47 0.1499619888786512 1101 48-49 0.23014157524956147 1101 50-51 0.1846486115299868 1101 52-53 0.22131719902676394 1101 54-55 0.2756102035040513 1101 56-57 0.25623543836641716 1101 58-59 0.24247103483131127 1101 60-61 0.10057738474566236 1101 62-63 0.1016598980159884 1101 64-65 0.17211187312198462 1101 66-67 -0.022812464907680408 1101 68-69 -0.055793686043877244 1101 70-71 -0.1049081811593382 1101 72-73 -0.15365507111947352 1101 74-75 -0.22339725394611776 1101 76-77 -0.11004427697420383 1101 78-79 -0.12923806723130937 1101 80-81 -0.24747092468131626 1101 82-83 -0.32462989974902356 1101 84-85 -0.39070726856888527 1101 86-87 -0.44330997828641117 1101 88-89 -0.6087681434462624 1101 90-91 -0.7816519173203638 1101 92-93 -0.7768779490783579 1101 94-95 -1.0799406356287342 1101 96-97 -1.160595167876398 1101 98-99 -1.338958719770698 1101 100-101 -1.3641728469906518 1102 1 0.15956060093711244 1102 2 0.17578699936810693 1102 3 0.22917978870616196 1102 4 0.1329700538022749 1102 5 0.19183102708254296 1102 6 0.1517419934439772 1102 7 0.15174046856330392 1102 8 0.2166101815283099 1102 9 0.259676546707162 1102 10-11 0.22739165427132235 1102 12-13 0.10914267564910318 1102 14-15 0.2689356363999238 1102 16-17 0.385219713168496 1102 18-19 0.3904591261359869 1102 20-21 0.46141524902485287 1102 22-23 0.3328072768655872 1102 24-25 0.2777133929360289 1102 26-27 0.33888104599449065 1102 28-29 0.39331929562737855 1102 30-31 0.43914400754848515 1102 32-33 0.26256491050502007 1102 34-35 0.15531935872949276 1102 36-37 0.0696845857912578 1102 38-39 0.06356904595887158 1102 40-41 0.052669326431420416 1102 42-43 0.11850846924460967 1102 44-45 0.022566673704901064 1102 46-47 -0.10023722941680546 1102 48-49 -0.11873251670981233 1102 50-51 -0.21791078513208362 1102 52-53 -0.13193657858847985 1102 54-55 -0.1737400383630714 1102 56-57 -0.049160754096632786 1102 58-59 -0.023912657008374083 1102 60-61 -0.15191868803057673 1102 62-63 -0.08558192338468018 1102 64-65 -0.20688989516765588 1102 66-67 -0.3501844872824691 1102 68-69 -0.3009493308566178 1102 70-71 -0.3671971188556995 1102 72-73 -0.28036569914281273 1102 74-75 -0.41249619505023105 1102 76-77 -0.5800007554388884 1102 78-79 -0.4271875992890912 1102 80-81 -0.48242072270980785 1102 82-83 -0.651200113938561 1102 84-85 -0.6052526456654839 1102 86-87 -0.537916071509148 1102 88-89 -0.49479398000725894 1102 90-91 -0.6099086039902453 1102 92-93 -0.6509654929219693 1102 94-95 -0.8876243320397883 1102 96-97 -1.0912653828970953 1102 98-99 -1.1175697916326577 1102 100-101 -1.3217925961470272 1103 1 0.3016534226964467 1103 2 0.21602259781919741 1103 3 0.320316564201768 1103 4 0.34615393003328165 1103 5 0.28668095228582047 1103 6 0.30208215766256075 1103 7 0.28850898081435616 1103 8 0.3160057721380909 1103 9 0.3839343781658755 1103 10-11 0.47815146825470123 1103 12-13 0.30948815220572357 1103 14-15 0.3949432971405429 1103 16-17 0.44573746909724576 1103 18-19 0.3682443251142189 1103 20-21 0.438450485538624 1103 22-23 0.4542251274187947 1103 24-25 0.46611384046933324 1103 26-27 0.3650925708250625 1103 28-29 0.43930394122460115 1103 30-31 0.4319389308487942 1103 32-33 0.4546555624361446 1103 34-35 0.3624633809525264 1103 36-37 0.2518389209334444 1103 38-39 0.38792768713761916 1103 40-41 0.26788885069534274 1103 42-43 0.39818311472709667 1103 44-45 0.2733880908571251 1103 46-47 0.26899969437533144 1103 48-49 0.20100638561648765 1103 50-51 0.2139336636243101 1103 52-53 0.10426219164267536 1103 54-55 0.06971693194820006 1103 56-57 0.032314549517202806 1103 58-59 -0.06476628239227722 1103 60-61 -0.07504366963691922 1103 62-63 0.04261003109182582 1103 64-65 -0.09826156080261939 1103 66-67 0.011620400766865657 1103 68-69 -0.07346553847575166 1103 70-71 -0.07680377139936212 1103 72-73 -0.1829880487724722 1103 74-75 -0.2699798826807651 1103 76-77 -0.21085860575266224 1103 78-79 -0.14448093063382927 1103 80-81 -0.22725191755689167 1103 82-83 -0.34117131757469465 1103 84-85 -0.349276764179006 1103 86-87 -0.26979663418800115 1103 88-89 -0.2979686132688819 1103 90-91 -0.25938707206159606 1103 92-93 -0.4226003013560664 1103 94-95 -0.29867060325235784 1103 96-97 -0.37807552093308416 1103 98-99 -0.7887078839895274 1103 100-101 -0.8801008683801577 1104 1 0.07428929073471124 1104 2 0.1192206878694293 1104 3 0.22530030936121648 1104 4 0.11763858362915869 1104 5 0.24761170219853312 1104 6 0.21003158875515027 1104 7 0.15225504317588445 1104 8 0.1624307878310134 1104 9 0.19496903514767894 1104 10-11 0.20039468908087343 1104 12-13 0.17005384659166367 1104 14-15 0.25018588544714504 1104 16-17 0.3352011914341233 1104 18-19 0.3673421322356134 1104 20-21 0.22678791960314015 1104 22-23 0.36779687468568056 1104 24-25 0.3166754477318321 1104 26-27 0.4242480395259136 1104 28-29 0.4146217498924756 1104 30-31 0.24881682959667017 1104 32-33 0.22654124400840203 1104 34-35 0.3146493196149365 1104 36-37 0.36739453198720895 1104 38-39 0.49036392008731866 1104 40-41 0.44787837113791795 1104 42-43 0.46701224270977804 1104 44-45 0.4550403165143706 1104 46-47 0.3292249712407269 1104 48-49 0.3896686763555337 1104 50-51 0.39647703588057226 1104 52-53 0.4179102542933322 1104 54-55 0.4477770515649979 1104 56-57 0.34731554128100584 1104 58-59 0.37082166064158884 1104 60-61 0.3677716620228182 1104 62-63 0.39139774993423515 1104 64-65 0.30460514085994106 1104 66-67 0.21827804150247232 1104 68-69 0.39489599568918976 1104 70-71 0.4959909709557664 1104 72-73 0.4196520600766007 1104 74-75 0.445509498722771 1104 76-77 0.3478353205705744 1104 78-79 0.23534443248859915 1104 80-81 0.30555289132086116 1104 82-83 0.21719890472927972 1104 84-85 0.16275119347436373 1104 86-87 0.18502264147382874 1104 88-89 0.17122643473200583 1104 90-91 0.17710522507948312 1104 92-93 0.13237030833613161 1104 94-95 0.07024007509006935 1104 96-97 0.010472560880465664 1104 98-99 0.11248146413108984 1104 100-101 0.04819220766771082 1105 1 -0.09239866851540768 1105 2 -0.04098745743664978 1105 3 0.0700137272146577 1105 4 -0.024181927676750092 1105 5 0.12188609445554022 1105 6 0.0428518395130979 1105 7 0.04205562096043991 1105 8 0.060513035814970806 1105 9 -0.08777769895924337 1105 10-11 0.04067169414393135 1105 12-13 0.005700287135674387 1105 14-15 0.08029671219923529 1105 16-17 0.02049978442936151 1105 18-19 0.07693487608949567 1105 20-21 0.16106526992658132 1105 22-23 0.147318354781234 1105 24-25 0.1298071563265708 1105 26-27 0.008196453777927104 1105 28-29 0.023454783526972278 1105 30-31 -0.02382783290214263 1105 32-33 -0.032087861625250014 1105 34-35 -0.04981766291461298 1105 36-37 -0.12530142503198505 1105 38-39 -0.10462756058977618 1105 40-41 -0.15803483113383265 1105 42-43 -0.2992350287486829 1105 44-45 -0.5557586865348298 1105 46-47 -0.48430992272449913 1105 48-49 -0.32664359472057214 1105 50-51 -0.40477936391447145 1105 52-53 -0.5101375271797366 1105 54-55 -0.38612156551604926 1105 56-57 -0.4451055811449436 1105 58-59 -0.48347387766926886 1105 60-61 -0.32408733620005137 1105 62-63 -0.27957230811438905 1105 64-65 -0.3700322784972556 1105 66-67 -0.4365117136969232 1105 68-69 -0.4270233681525255 1105 70-71 -0.4810302103567565 1105 72-73 -0.4534629166686841 1105 74-75 -0.5132458513250029 1105 76-77 -0.3856836474580412 1105 78-79 -0.6570545273343562 1105 80-81 -0.6759685805324018 1105 82-83 -0.5896132566785752 1105 84-85 -0.6731834982800038 1105 86-87 -0.5078316133109375 1105 88-89 -0.5281140446670776 1105 90-91 -0.5885410460396692 1105 92-93 -0.5842416615758452 1105 94-95 -0.7260856034450391 1105 96-97 -0.5171565122419288 1105 98-99 -0.7768992147497329 1105 100-101 -0.5976095588382755 1106 1 0.2589773196657106 1106 2 0.23808824941735907 1106 3 0.10518092562622883 1106 4 0.2052751686293277 1106 5 0.21352303055709143 1106 6 0.21644559688025566 1106 7 0.1760121174163487 1106 8 0.1150823748272316 1106 9 0.15402740474411303 1106 10-11 0.21211358726058194 1106 12-13 0.14231550813725136 1106 14-15 0.15134883367806395 1106 16-17 0.36750786799975543 1106 18-19 0.2822931883363893 1106 20-21 0.3014537842365428 1106 22-23 0.41510757948144317 1106 24-25 0.45600253459890183 1106 26-27 0.38832792312270215 1106 28-29 0.3605784364835216 1106 30-31 0.24590042711326277 1106 32-33 0.2956673890283028 1106 34-35 0.3270264179525242 1106 36-37 0.19446062566975542 1106 38-39 0.29415885607820513 1106 40-41 0.1987863034997872 1106 42-43 0.04855364587058375 1106 44-45 -0.08915776763771532 1106 46-47 0.012016977281270158 1106 48-49 -0.0035705806437462684 1106 50-51 -0.044959633296961954 1106 52-53 -0.20290684418876026 1106 54-55 -0.1338348004634824 1106 56-57 0.04928883212824786 1106 58-59 0.0104221884661726 1106 60-61 0.01806235392102451 1106 62-63 -0.051164294744801 1106 64-65 -0.08550749604117414 1106 66-67 -0.05691984756042956 1106 68-69 0.0550996561400936 1106 70-71 -0.0053261067529462025 1106 72-73 -0.06496366844596935 1106 74-75 0.0031714453153099953 1106 76-77 0.0045018607905547015 1106 78-79 -0.06407906094430516 1106 80-81 0.12793345791039812 1106 82-83 0.06366417795347346 1106 84-85 0.19115540920685703 1106 86-87 0.040802745268489815 1106 88-89 0.13609886809008742 1106 90-91 0.3282218847915779 1106 92-93 0.30867853477685614 1106 94-95 0.11929122819352145 1106 96-97 0.1576854049735097 1106 98-99 0.09956177403385524 1106 100-101 0.06905252382055593 1107 1 0.09965940086045322 1107 2 0.1880652376813714 1107 3 0.1290756119162424 1107 4 0.1267884737482703 1107 5 0.2158984086665896 1107 6 0.2624482248872013 1107 7 0.21615342281543803 1107 8 0.29563195511317986 1107 9 0.20236205541595353 1107 10-11 0.3123997331950079 1107 12-13 0.2918476028388781 1107 14-15 0.46144948501024174 1107 16-17 0.38268477488892927 1107 18-19 0.3587038244274581 1107 20-21 0.32082199535754796 1107 22-23 0.4749902029527462 1107 24-25 0.4456280769588332 1107 26-27 0.4639127213698089 1107 28-29 0.3858504918984522 1107 30-31 0.3557231033448076 1107 32-33 0.37179454721231053 1107 34-35 0.5602457764920459 1107 36-37 0.467411497280942 1107 38-39 0.6131156907305453 1107 40-41 0.5197946010648735 1107 42-43 0.5274312067599638 1107 44-45 0.7866230472445466 1107 46-47 0.7995757155711303 1107 48-49 0.7267627226742874 1107 50-51 0.5629078791343716 1107 52-53 0.7359019482954068 1107 54-55 0.8212039359587635 1107 56-57 0.7115457498229389 1107 58-59 0.6946749073528125 1107 60-61 0.6614050420252866 1107 62-63 0.7786705877119147 1107 64-65 0.5882698804208175 1107 66-67 0.43235009677784575 1107 68-69 0.48340017273689284 1107 70-71 0.3752984284266674 1107 72-73 0.40291369901143526 1107 74-75 0.521028885943366 1107 76-77 0.44585361685280844 1107 78-79 0.472576299956426 1107 80-81 0.5737112212363549 1107 82-83 0.686623553901871 1107 84-85 0.7503485143571496 1107 86-87 0.6099584313350555 1107 88-89 0.6994074054441342 1107 90-91 0.7438367650471776 1107 92-93 0.6654871738494492 1107 94-95 0.5962098733692294 1107 96-97 0.6501332200258538 1107 98-99 0.6801887271132578 1107 100-101 0.4691903441088989 1108 1 -0.0028711404993373435 1108 2 0.048567601724023746 1108 3 0.03337311085072514 1108 4 -0.010188180583682538 1108 5 0.028864196767116823 1108 6 0.16272744879609746 1108 7 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1435.0 5 1513.0 6 1719.0 7 2052.0 8 2594.0 9 3153.0 10 3733.0 11 4717.0 12 5947.0 13 9250.0 14 7970.0 15 7918.0 16 9495.0 17 12998.0 18 12225.0 19 8189.0 20 8388.0 21 8581.0 22 9377.0 23 10121.0 24 11339.0 25 13143.0 26 14840.0 27 17344.0 28 20548.0 29 24290.0 30 29390.0 31 35835.0 32 45445.0 33 59678.0 34 82861.0 35 122576.0 36 203894.0 37 351089.0 38 380297.0 39 75933.0 40 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.51007548051983 18.90948320794368 14.406620228187167 23.17382108334932 2 15.07556399121641 20.045259618130434 41.32460390141108 23.55457248924207 3 19.479472506210897 24.487389590065522 29.483552589067962 26.549585314655623 4 12.93637883803816 16.421316563486073 36.935268561890815 33.70703603658495 5 14.07038578422109 37.124737082818065 33.942708214711544 14.8621689182493 6 32.08063840376909 35.35349744590505 17.882602069976603 14.68326208034925 7 28.73033794280968 32.52815677346911 20.697728076787005 18.043777206934212 8 25.850470314529574 35.72224522809347 19.854306176081675 18.57297828129528 9 26.4599428198419 17.475139797303907 20.595315952058247 35.46960143079595 10-11 25.119631207349276 26.682342692191273 27.07691957485241 21.121106525607047 12-13 25.874698102835303 23.978733150131372 27.80850114114129 22.338067605892036 14-15 22.87470248157362 25.344709147955218 26.178914361145612 25.601674009325553 16-17 22.291257369653223 28.57470668264944 26.80337532962924 22.330660618068094 18-19 22.152978408625597 27.194370362075375 29.062353580713925 21.5902976485851 20-21 23.049646590217872 26.383848589914503 29.0048621728002 21.56164264706742 22-23 22.661489532704067 26.343997351329197 28.809402781350503 22.185110334616233 24-25 22.418027804919333 26.50145795025274 29.012456049916523 22.06805819491141 26-27 22.26582611545628 26.71247947732774 28.859054826117 22.162639581098983 28-29 22.111163424759127 26.768585962094143 29.040136877121398 22.080113736025332 30-31 22.26991454458909 26.59425433669364 28.927639670042975 22.20819144867429 32-33 22.127548289038895 26.689701004855053 28.834294293924717 22.34845641218133 34-35 22.56140056568328 26.7693946350134 28.628403073389574 22.04080172591375 36-37 22.55927200952615 26.497611512924703 28.701259187528283 22.241857290020867 38-39 22.358889508559795 26.84250703614699 28.725977201463248 22.07262625382997 40-41 22.510083284347385 26.651948241198582 28.72232499666566 22.11564347778837 42-43 22.37710684784616 26.668229410845445 28.486942438510958 22.467721302797436 44-45 22.41085355118682 26.901270983583775 28.377619569749662 22.31025589547975 46-47 22.441385961048457 26.998335396198254 28.05686773396116 22.50341090879213 48-49 22.550396732842866 27.027971325876994 27.99254880578549 22.429083135494654 50-51 22.49858066078503 27.22175236963238 27.86050134953058 22.419165620052002 52-53 22.646380701026036 27.252671352035268 27.639288397287626 22.46165954965107 54-55 22.391723118062874 27.135802400500843 27.80948198141853 22.662992500017758 56-57 22.411860011060632 27.4235737204081 27.70641958207363 22.458146686457642 58-59 22.470279808793002 27.11511318184793 27.833281674678904 22.581325334680162 60-61 22.532442203934416 26.965664896998394 28.103502758189087 22.398390140878103 62-63 22.423860050911802 27.09927801345769 28.02227445370797 22.45458748192254 64-65 22.334355954251745 26.953820185739925 27.978469664425674 22.73335419558266 66-67 22.23456959469312 27.228860350908413 27.813594426196286 22.72297562820218 68-69 22.303761853603767 27.065595991673625 27.896008698384627 22.73463345633798 70-71 22.218607446129795 26.99629099256499 27.912003373791023 22.87309818751419 72-73 22.290447617426473 27.009409687971793 27.805984770386498 22.894157924215232 74-75 22.253309560582053 27.19973899817688 27.651888818413727 22.895062622827343 76-77 22.27962934362934 27.011799227799226 27.743166023166022 22.965405405405406 78-79 22.26204880109542 27.0537760723067 27.756850552435512 22.927324574162363 80-81 22.21320134928103 27.113810304527036 27.692347125798563 22.980641220393377 82-83 22.318536281624574 27.117385578279702 27.61938207452762 22.9446960655681 84-85 22.341339298041117 26.953115951361934 27.618647865729834 23.086896884867116 86-87 22.270560407906338 26.852680653544624 27.775615686405324 23.101143252143714 88-89 22.264687047929545 26.807208352678817 27.68469805130315 23.24340654808848 90-91 22.127376519246805 26.89229432644601 27.81542049217124 23.164908662135947 92-93 22.218913185252557 26.71147665671793 27.802696817073723 23.26691334095579 94-95 22.134154969392554 26.679207882861867 27.78592026554671 23.400716882198864 96-97 22.17211806798848 26.51246766495315 27.819123316669824 23.496290950388545 98-99 22.074976421003335 26.451201130926837 27.68997504163589 23.78384740643394 100-101 22.31193739679237 25.981387567700807 28.062281962781316 23.64439307272551 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1507.0 1 864.5 2 228.0 3 255.0 4 245.0 5 267.0 6 419.0 7 746.0 8 1235.0 9 1542.0 10 1572.0 11 1603.0 12 1748.5 13 1982.0 14 2166.5 15 2334.5 16 2457.0 17 2529.5 18 2611.5 19 2689.5 20 2868.0 21 3213.5 22 3789.0 23 4569.5 24 5498.5 25 6739.5 26 8564.0 27 10854.5 28 12933.0 29 15041.0 30 17863.5 31 21253.0 32 24543.5 33 27197.5 34 30113.5 35 33429.0 36 36734.5 37 40398.0 38 43253.0 39 45013.5 40 46940.5 41 50011.5 42 53026.0 43 55097.5 44 57763.5 45 60576.5 46 61694.5 47 62397.0 48 66162.5 49 67043.5 50 64223.0 51 62137.0 52 59755.0 53 58177.5 54 56093.0 55 51576.0 56 45975.0 57 40759.5 58 36253.0 59 31744.5 60 26402.5 61 21449.5 62 17398.5 63 13460.0 64 9835.5 65 7167.5 66 5190.5 67 4012.5 68 3128.5 69 2305.0 70 1741.0 71 1291.5 72 1004.0 73 821.5 74 630.0 75 459.5 76 330.0 77 207.0 78 131.5 79 98.0 80 63.5 81 42.0 82 34.0 83 25.5 84 16.0 85 15.5 86 14.5 87 8.0 88 7.0 89 6.5 90 5.0 91 3.5 92 7.0 93 7.0 94 2.5 95 3.5 96 3.0 97 2.5 98 2.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3470491296418436 2 0.12361243352988797 3 0.11629772463378817 4 0.1252720733634568 5 0.1412537902793051 6 0.08789944303716547 7 0.09736553690270638 8 0.10265179711333311 9 0.09035816871652676 10-11 0.08894440145089402 12-13 0.0929705647508481 14-15 0.09463020458441697 16-17 0.08350447088530721 18-19 0.09856416567139499 20-21 0.10833760024685606 22-23 0.11635919277577221 24-25 0.13194136676872428 26-27 0.1310193446389638 28-29 0.12564088221536102 30-31 0.11430001001930715 32-33 0.10750778033007163 34-35 0.3354316508068616 36-37 0.5013649000927555 38-39 0.47453405611672556 40-41 0.45160643915668164 42-43 0.4863359393776597 44-45 0.49383505269971156 46-47 0.5013034319507714 48-49 0.5118144842300408 50-51 0.5008424208858911 52-53 0.5042231686950129 54-55 0.4910382522394381 56-57 0.46731154943360176 58-59 0.44582843381018267 60-61 0.40525946010072167 62-63 0.4784987512746956 64-65 0.5105236532483762 66-67 0.5182379050673721 68-69 0.5303163949672344 70-71 0.5225714090772464 72-73 0.5143346780513861 74-75 0.5212191099535978 76-77 0.4984451633485139 78-79 0.4767469092281507 80-81 0.47788406985485526 82-83 0.4931589031378872 84-85 0.4919602743691986 86-87 0.49518735182336027 88-89 0.5080034594270308 90-91 0.5224484727932783 92-93 0.516055786026939 94-95 0.5201434174688772 96-97 0.5195594701200288 98-99 0.51408880548345 100-101 0.5013341660217634 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1626859.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.55756605479289 #Duplication Level Percentage of deduplicated Percentage of total 1 80.83369013155898 52.99259980252338 2 11.749097347068663 15.404844508292914 3 3.466079032455433 6.816831153639891 4 1.4117209432867877 3.7019595596183246 5 0.6877091130861167 2.254226780381306 6 0.4280175482837035 1.6835873216531634 7 0.2770717384832889 1.2714904158274156 8 0.19787371853064634 1.0377695498464272 9 0.14497921272667239 0.8554035883410626 >10 0.7356330929030153 8.604782724865135 >50 0.046404050053585416 2.0578933525221816 >100 0.02097181662055722 2.411073495637776 >500 3.761274712981796E-4 0.14511233909028068 >1k 3.761274712981796E-4 0.762425407760659 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4918 0.30230032227746845 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3986 0.24501201394835076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2378 0.14617124163802764 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 6.14681419840318E-5 0.0 0.0 0.0 0.0 12-13 6.14681419840318E-5 0.0 0.0 0.0 0.0 14-15 6.14681419840318E-5 0.0 0.0 3.07340709920159E-5 0.0 16-17 6.14681419840318E-5 0.0 0.0 1.229362839680636E-4 0.0 18-19 9.220221297604771E-5 0.0 0.0 1.229362839680636E-4 0.0 20-21 1.229362839680636E-4 0.0 0.0 2.1513849694411132E-4 0.0 22-23 1.229362839680636E-4 0.0 0.0 3.6880885190419084E-4 3.07340709920159E-5 24-25 1.229362839680636E-4 0.0 0.0 7.376177038083817E-4 6.14681419840318E-5 26-27 1.229362839680636E-4 0.0 0.0 9.83490271744509E-4 6.14681419840318E-5 28-29 1.8440442595209542E-4 0.0 0.0 0.0032885455961457013 6.14681419840318E-5 30-31 2.458725679361272E-4 0.0 0.0 0.0100500412143892 6.14681419840318E-5 32-33 2.458725679361272E-4 0.0 0.0 0.02249733996615564 6.14681419840318E-5 34-35 3.0734070992015903E-4 0.0 0.0 0.040538239638468976 6.14681419840318E-5 36-37 3.6880885190419084E-4 0.0 0.0 0.06961267079691602 6.14681419840318E-5 38-39 4.3027699388822264E-4 0.0 0.0 0.11399266930938699 6.14681419840318E-5 40-41 4.6101106488023855E-4 0.0 0.0 0.1656259085759737 6.14681419840318E-5 42-43 4.917451358722544E-4 6.14681419840318E-5 0.0 0.21759722262347259 6.14681419840318E-5 44-45 4.917451358722544E-4 6.14681419840318E-5 0.0 0.28238464427464216 6.14681419840318E-5 46-47 5.532132778562863E-4 6.14681419840318E-5 0.0 0.36155561115007506 6.14681419840318E-5 48-49 5.532132778562863E-4 6.14681419840318E-5 0.0 0.44785688249565575 6.14681419840318E-5 50-51 5.532132778562863E-4 6.14681419840318E-5 0.0 0.5407967131755118 6.14681419840318E-5 52-53 5.532132778562863E-4 6.14681419840318E-5 0.0 0.6362874717477052 6.14681419840318E-5 54-55 5.532132778562863E-4 6.14681419840318E-5 0.0 0.7421663463152 6.14681419840318E-5 56-57 5.532132778562863E-4 6.14681419840318E-5 0.0 0.8722022006824193 6.14681419840318E-5 58-59 5.532132778562863E-4 6.14681419840318E-5 0.0 1.016621600273902 6.14681419840318E-5 60-61 5.532132778562863E-4 6.14681419840318E-5 0.0 1.1776373982010733 6.14681419840318E-5 62-63 5.532132778562863E-4 6.14681419840318E-5 0.0 1.339206409406101 6.14681419840318E-5 64-65 5.532132778562863E-4 6.14681419840318E-5 0.0 1.5036644232843779 6.14681419840318E-5 66-67 7.376177038083817E-4 6.14681419840318E-5 0.0 1.6983647630187988 6.14681419840318E-5 68-69 7.990858457924135E-4 6.14681419840318E-5 0.0 1.9238299078162275 6.14681419840318E-5 70-71 7.990858457924135E-4 6.14681419840318E-5 0.0 2.176740578009526 6.14681419840318E-5 72-73 8.298199167844294E-4 6.14681419840318E-5 0.0 2.4361054031111484 6.14681419840318E-5 74-75 8.605539877764453E-4 6.14681419840318E-5 0.0 2.705612471640136 6.14681419840318E-5 76-77 8.605539877764453E-4 6.14681419840318E-5 0.0 3.0033948854817782 6.14681419840318E-5 78-79 8.605539877764453E-4 6.14681419840318E-5 0.0 3.337474237164991 6.14681419840318E-5 80-81 0.0010142243427365247 6.14681419840318E-5 0.0 3.706989972701998 6.14681419840318E-5 82-83 0.0011064265557125725 6.14681419840318E-5 0.0 4.091165860102198 6.14681419840318E-5 84-85 0.0011064265557125725 6.14681419840318E-5 0.0 4.487389503331266 6.14681419840318E-5 86-87 0.0011064265557125725 6.14681419840318E-5 0.0 4.909276095838669 6.14681419840318E-5 88-89 0.0011064265557125725 6.14681419840318E-5 0.0 5.359714640297653 6.14681419840318E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4540 0.0 35.655655 1 GTATCAA 6680 0.0 33.613564 1 ATCAACG 8245 0.0 26.4893 3 TCAACGC 8305 0.0 26.240881 4 TATCAAC 8445 0.0 26.14246 2 CAACGCA 8360 0.0 26.011572 5 AACGCAG 8690 0.0 25.124321 6 GTACATG 7040 0.0 23.46586 1 TACATGG 7115 0.0 23.371813 2 ACGCAGA 9835 0.0 22.103014 7 ACATGGG 7145 0.0 22.080158 3 CGCAGAG 9940 0.0 21.774239 8 GAGTACT 4790 0.0 21.108736 12-13 TACCTGG 1400 0.0 20.642485 2 ACGATAC 165 6.4530286E-5 20.098991 3 GTACTTT 5035 0.0 20.036415 14-15 AGTACTT 4945 0.0 19.68092 12-13 GTGTAGC 870 0.0 19.64324 1 GCAGAGT 10745 0.0 19.613424 9 GTACCTG 1600 0.0 19.285162 1 >>END_MODULE