FastQCFastQC Report
Fri 27 May 2016
SRR939161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939161_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626859
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48730.299534255888187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40710.25023680601699344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25010.15373182310206354No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67050.033.3120231
GGTATCA45250.032.3107261
ATCAACG80950.027.3143793
TCAACGC82400.026.776144
TATCAAC83550.026.577962
CAACGCA83750.026.3445245
AACGCAG85800.025.7150826
GTACATG69400.023.6748121
TACATGG70000.022.7752672
ACGCAGA97450.022.5435057
CGCAGAG98100.022.2006638
GTGTAGC9900.022.128381
ACATGGG69900.021.9254023
GAGTACT50650.020.42393112-13
GCAGAGT104950.020.3459999
CATGGGG33150.019.895444
AGTACTT53000.019.65264312-13
GTACTTT52950.019.31272714-15
AGAGTAC90200.019.09687810-11
GAGTACA61650.018.8487991
Produced by FastQC (version 0.11.5)