##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939161_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1626859 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.54438153521602 34.0 31.0 34.0 31.0 34.0 2 32.75297428972025 34.0 31.0 34.0 31.0 34.0 3 32.84631058991591 34.0 31.0 34.0 31.0 34.0 4 36.29104550548019 37.0 37.0 37.0 35.0 37.0 5 36.25071011071027 37.0 37.0 37.0 35.0 37.0 6 36.28489192978617 37.0 37.0 37.0 35.0 37.0 7 36.237456964617095 37.0 37.0 37.0 35.0 37.0 8 36.22556718191312 37.0 37.0 37.0 35.0 37.0 9 37.98436188999784 39.0 38.0 39.0 35.0 39.0 10-11 37.92745130340121 39.0 38.0 39.0 35.0 39.0 12-13 37.86329638893106 39.0 38.0 39.0 35.0 39.0 14-15 39.25384805935856 41.0 39.0 41.0 36.0 41.0 16-17 39.19601483595075 40.0 39.0 41.0 36.0 41.0 18-19 39.219456941259196 40.5 38.5 41.0 36.0 41.0 20-21 39.285982374624965 41.0 39.0 41.0 36.0 41.0 22-23 39.24684192053522 41.0 39.0 41.0 36.0 41.0 24-25 39.20109948065567 41.0 39.0 41.0 36.0 41.0 26-27 39.07686898495813 40.5 39.0 41.0 35.5 41.0 28-29 38.937626432284546 40.0 38.5 41.0 35.0 41.0 30-31 38.76191728969751 40.0 38.0 41.0 35.0 41.0 32-33 38.64377890155201 40.0 38.0 41.0 35.0 41.0 34-35 38.609658857958806 40.0 38.0 41.0 35.0 41.0 36-37 38.5206554470916 40.0 38.0 41.0 34.5 41.0 38-39 38.402324048980276 40.0 38.0 41.0 34.0 41.0 40-41 38.24710684822716 40.0 38.0 41.0 34.0 41.0 42-43 38.09812958590757 40.0 38.0 41.0 33.5 41.0 44-45 37.891135925117055 40.0 38.0 41.0 33.0 41.0 46-47 37.62259236971366 40.0 37.5 41.0 33.0 41.0 48-49 37.34012505078805 40.0 37.0 41.0 32.5 41.0 50-51 37.10409537642783 40.0 37.0 41.0 31.5 41.0 52-53 36.88438026897229 40.0 36.5 41.0 31.0 41.0 54-55 36.653948498302555 39.5 36.0 41.0 31.0 41.0 56-57 36.39804094884683 39.0 35.5 41.0 30.5 41.0 58-59 36.11279465522212 39.0 35.0 40.5 30.0 41.0 60-61 35.77824015480137 38.5 35.0 40.0 29.0 41.0 62-63 35.39863934120904 38.0 35.0 40.0 28.5 41.0 64-65 34.998680893673026 37.5 34.0 40.0 28.0 41.0 66-67 34.65098143108899 37.0 34.0 39.5 28.0 41.0 68-69 34.196473388289945 36.0 34.0 39.0 26.5 40.5 70-71 33.77514769257815 36.0 33.5 39.0 26.0 40.0 72-73 33.32051025934024 35.0 33.0 38.0 26.0 40.0 74-75 32.83009375735697 35.0 33.0 37.0 25.5 39.5 76-77 30.831786897327916 33.5 30.0 35.5 21.5 38.0 78-79 31.801592823963233 34.5 31.5 36.0 23.5 39.0 80-81 31.83139534526348 35.0 32.0 36.0 24.0 37.5 82-83 31.542438834588616 35.0 32.0 35.5 23.5 37.0 84-85 31.17807013392064 35.0 32.0 35.0 21.5 37.0 86-87 30.638015648559588 34.0 31.0 35.0 19.0 36.0 88-89 30.120019927971633 34.0 31.0 35.0 14.5 36.0 90-91 29.69162293720599 34.0 30.0 35.0 8.5 35.5 92-93 29.426466276425923 34.0 30.0 35.0 4.5 35.0 94-95 29.12231883648183 34.0 30.0 35.0 2.0 35.0 96-97 28.61997966633863 34.0 29.0 35.0 2.0 35.0 98-99 27.907965902392277 34.0 29.0 35.0 2.0 35.0 100-101 24.36711294586685 30.0 14.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22898308232921494 1101 2 0.18790551774988984 1101 3 0.24321320286051673 1101 4 0.16963590312400356 1101 5 0.1508964196134528 1101 6 0.1634837905623172 1101 7 0.09004123609364001 1101 8 0.17876339207772673 1101 9 0.2744879973238241 1101 10-11 0.23030001694432656 1101 12-13 0.21051780418161314 1101 14-15 0.3047209441901799 1101 16-17 0.2622576848991329 1101 18-19 0.24544677816460592 1101 20-21 0.3045643903516364 1101 22-23 0.23195180860600573 1101 24-25 0.09402429197044881 1101 26-27 0.2704203673064498 1101 28-29 0.1479342174729581 1101 30-31 0.14014362954854676 1101 32-33 0.16357561608087678 1101 34-35 0.27565328567615666 1101 36-37 0.2979992287102178 1101 38-39 0.38122056242465874 1101 40-41 0.24517602111090753 1101 42-43 0.12348930045136797 1101 44-45 0.17390029811038943 1101 46-47 -0.016307345736535694 1101 48-49 -0.06202678394672034 1101 50-51 -0.0043613312601351595 1101 52-53 0.04616253474062404 1101 54-55 0.023089006570458537 1101 56-57 -0.04659405939788286 1101 58-59 0.01534428307396496 1101 60-61 0.06775409163061852 1101 62-63 0.06538677002795623 1101 64-65 0.22488052155234328 1101 66-67 0.3271239692798602 1101 68-69 0.17899240072603106 1101 70-71 0.03485932091558652 1101 72-73 -0.24250774198029745 1101 74-75 -0.14570296959394113 1101 76-77 -0.09468231164790453 1101 78-79 -0.027883369032984717 1101 80-81 -0.15151744197971695 1101 82-83 -0.0538874071576636 1101 84-85 -0.10786052486803399 1101 86-87 -0.1793263562948404 1101 88-89 -0.029550158488472533 1101 90-91 0.0634914150533632 1101 92-93 -0.060813344709085726 1101 94-95 -0.20435392964879995 1101 96-97 -0.2585995132347705 1101 98-99 -0.2549539884307066 1101 100-101 -0.37857503347043675 1102 1 0.11529307119169374 1102 2 0.09642561931745064 1102 3 0.1605892979117982 1102 4 0.10755719657281304 1102 5 0.09481296299161102 1102 6 0.16951384525254554 1102 7 0.13371082151179792 1102 8 0.16759262225875915 1102 9 0.2134669399240181 1102 10-11 0.18344865301327218 1102 12-13 0.20012520647893695 1102 14-15 0.28642376971339445 1102 16-17 0.35304979969874495 1102 18-19 0.21861308674049695 1102 20-21 0.2951294430470881 1102 22-23 0.3648800214490677 1102 24-25 0.3674660776313061 1102 26-27 0.28715860018042605 1102 28-29 0.3226071046681298 1102 30-31 0.3625559215511416 1102 32-33 0.2766342065192333 1102 34-35 0.2892325968951326 1102 36-37 0.40270623496054014 1102 38-39 0.4671000112003654 1102 40-41 0.3497432888436691 1102 42-43 0.3938300858130077 1102 44-45 0.46948128826947055 1102 46-47 0.50804819476582 1102 48-49 0.4051606381321662 1102 50-51 0.380191796389191 1102 52-53 0.34730683216236713 1102 54-55 0.2149525885309913 1102 56-57 0.3824790326778569 1102 58-59 0.34832350826804515 1102 60-61 0.27272062711277556 1102 62-63 0.13184983750139878 1102 64-65 0.09367202752837045 1102 66-67 0.2529563225388287 1102 68-69 0.28666196744312344 1102 70-71 0.24872107017169753 1102 72-73 0.18985414026116842 1102 74-75 0.23098921710086984 1102 76-77 0.23468189368148273 1102 78-79 0.26887890509969736 1102 80-81 0.10059214210858158 1102 82-83 0.18145771061749016 1102 84-85 0.16551398890426228 1102 86-87 0.2116074710236795 1102 88-89 0.27699069502816087 1102 90-91 0.4057926019476561 1102 92-93 0.2807499293072375 1102 94-95 -0.033039701878134764 1102 96-97 -0.09484233410349674 1102 98-99 0.03163204893817806 1102 100-101 -0.43716858866767083 1103 1 0.1565906384401572 1103 2 0.10662630598078948 1103 3 0.15220079191583835 1103 4 0.13831388799879818 1103 5 0.15616772391894074 1103 6 0.18987581250360108 1103 7 0.18523052430020215 1103 8 0.20677423288981345 1103 9 0.2390499627890179 1103 10-11 0.21179010117158725 1103 12-13 0.1633762975034827 1103 14-15 0.3251684218350661 1103 16-17 0.20942039039581317 1103 18-19 0.315676486128055 1103 20-21 0.2705866906973142 1103 22-23 0.28388282825903133 1103 24-25 0.2698963849937073 1103 26-27 0.2820941712467899 1103 28-29 0.3251677513667204 1103 30-31 0.2647779752860586 1103 32-33 0.20865074509952564 1103 34-35 0.24072671067493445 1103 36-37 0.3668317418149485 1103 38-39 0.3625696592941381 1103 40-41 0.3353640617314966 1103 42-43 0.2793886070775713 1103 44-45 0.1465789260498198 1103 46-47 0.288303955160913 1103 48-49 0.10939609292566388 1103 50-51 0.12669301074476635 1103 52-53 0.24975335088499406 1103 54-55 0.1987095538042638 1103 56-57 0.3320756690949551 1103 58-59 0.32845336874559194 1103 60-61 0.376596788282626 1103 62-63 0.2738416500162657 1103 64-65 0.20934731547954044 1103 66-67 0.1359970948580056 1103 68-69 0.16785414588580494 1103 70-71 0.15831505291382797 1103 72-73 0.09612005545231028 1103 74-75 0.172755267719225 1103 76-77 0.13584621851324385 1103 78-79 0.11645395995645202 1103 80-81 -0.0821744836389442 1103 82-83 0.036926201469544395 1103 84-85 -0.15169668944389514 1103 86-87 -0.02245763479659857 1103 88-89 0.1469566312192505 1103 90-91 0.2695997176872922 1103 92-93 0.159757065797411 1103 94-95 0.05884005272676518 1103 96-97 0.04039283287600881 1103 98-99 0.1433053755641609 1103 100-101 -0.07284445523970007 1104 1 0.033913538962643486 1104 2 0.02370284565206049 1104 3 0.064497654436245 1104 4 0.02695346182373015 1104 5 0.052016195858271885 1104 6 0.007003213088282223 1104 7 0.09797740264560417 1104 8 0.07743334518197287 1104 9 0.12698593663142788 1104 10-11 0.12841278283366364 1104 12-13 0.16314888822585516 1104 14-15 0.0696742184903485 1104 16-17 0.07728503408846166 1104 18-19 0.17114456262018507 1104 20-21 0.06331358007938093 1104 22-23 0.15100761135573038 1104 24-25 0.10472088602605112 1104 26-27 0.04176578936826303 1104 28-29 -0.004724825965645607 1104 30-31 0.012160127091725315 1104 32-33 0.1162824755856704 1104 34-35 0.22106769491765732 1104 36-37 0.2475295208883992 1104 38-39 0.2923547034960592 1104 40-41 0.3075447269960847 1104 42-43 0.22082675583637723 1104 44-45 0.13686660478943224 1104 46-47 0.13237010988855502 1104 48-49 0.022426092219667737 1104 50-51 0.2095055280045841 1104 52-53 0.026208830295928465 1104 54-55 0.08937227774957535 1104 56-57 0.20193344584388484 1104 58-59 0.2548942718702065 1104 60-61 0.3244826024102423 1104 62-63 0.27582397692947325 1104 64-65 0.2596609577789124 1104 66-67 0.33156571170530924 1104 68-69 0.1844679905242046 1104 70-71 0.2149613351923776 1104 72-73 0.10782647031502535 1104 74-75 0.004717821791956567 1104 76-77 -0.08091468572559535 1104 78-79 0.1420834898315917 1104 80-81 0.0386139421653553 1104 82-83 0.04382892553011786 1104 84-85 0.012556685314589089 1104 86-87 0.1577143893957107 1104 88-89 0.36420789421723754 1104 90-91 0.3499066505724997 1104 92-93 0.37943682934939815 1104 94-95 0.34536277516401626 1104 96-97 0.4078451621761694 1104 98-99 0.1420629092469099 1104 100-101 0.05562785870133169 1105 1 -0.09615346627899157 1105 2 -0.011276060953171907 1105 3 -0.0770169763160169 1105 4 -0.015926595908936747 1105 5 -0.027573191551809373 1105 6 -0.006397377334451448 1105 7 -0.03813990163091319 1105 8 -0.005642988958648232 1105 9 0.053486685992993444 1105 10-11 -0.007115655586588332 1105 12-13 0.017729930316946252 1105 14-15 0.07612266594048123 1105 16-17 -0.02093513488764387 1105 18-19 -0.0718931060205108 1105 20-21 -0.0010948821885534699 1105 22-23 0.008941434006366933 1105 24-25 -0.008879396776087845 1105 26-27 0.026312410738960068 1105 28-29 0.04877473604820892 1105 30-31 -0.015786274209830253 1105 32-33 0.06461293098595178 1105 34-35 0.20847393314091534 1105 36-37 0.1554468818535426 1105 38-39 0.23144646720425044 1105 40-41 0.23491480903892636 1105 42-43 0.2184662554560859 1105 44-45 0.33244585235074453 1105 46-47 0.3230667162170775 1105 48-49 0.36153452367059913 1105 50-51 0.33237701375526996 1105 52-53 0.26416741645007136 1105 54-55 0.3269299680583373 1105 56-57 0.1543722250874211 1105 58-59 0.17969436198591637 1105 60-61 0.32185514340304167 1105 62-63 0.24977719460515857 1105 64-65 -0.09829072316751564 1105 66-67 -0.08451907215665244 1105 68-69 -0.045559222771160535 1105 70-71 0.19229686830143322 1105 72-73 0.09397965144989229 1105 74-75 0.027010469806320714 1105 76-77 -0.05749215707333377 1105 78-79 0.0785890059844192 1105 80-81 0.0326089757275021 1105 82-83 -0.10089561941531855 1105 84-85 0.006859888955482063 1105 86-87 0.20426547167712172 1105 88-89 0.302366676446308 1105 90-91 0.45050517822469516 1105 92-93 0.27464824180779956 1105 94-95 0.3120744538941942 1105 96-97 0.1833927135519673 1105 98-99 0.26085789555326144 1105 100-101 -0.35089106033144546 1106 1 0.04055605431236842 1106 2 0.05572772674701554 1106 3 0.04789068866897139 1106 4 0.007690212469057656 1106 5 0.010556585639214688 1106 6 0.03817096629325789 1106 7 0.032380474938783266 1106 8 0.07511937083619813 1106 9 0.11177169078656135 1106 10-11 0.0960945905877324 1106 12-13 0.11266866177791002 1106 14-15 0.044879378237666856 1106 16-17 0.009852371464482701 1106 18-19 -0.0029404601311853185 1106 20-21 -0.02573130488121933 1106 22-23 0.10418598427590808 1106 24-25 0.05270478394383815 1106 26-27 0.14067554798153736 1106 28-29 0.06267148323624383 1106 30-31 0.04804930921649486 1106 32-33 0.10492011189971606 1106 34-35 -0.05055852687343787 1106 36-37 0.09847552273451754 1106 38-39 0.2517418344749558 1106 40-41 0.05615291026429503 1106 42-43 0.13825594117651718 1106 44-45 0.09386592559009443 1106 46-47 0.1431090462551623 1106 48-49 0.2075609747055367 1106 50-51 0.022156247937822116 1106 52-53 0.2357531164411668 1106 54-55 0.0989260022366949 1106 56-57 0.24023527588870053 1106 58-59 0.2494112274675544 1106 60-61 0.24096050223917587 1106 62-63 0.11733276587730757 1106 64-65 0.22900350726924046 1106 66-67 0.31735107206932867 1106 68-69 0.0267528460046762 1106 70-71 0.11264383840322267 1106 72-73 0.11010815559645692 1106 74-75 0.12992668462078427 1106 76-77 0.35463634183436454 1106 78-79 0.49759941037249433 1106 80-81 0.18765845681781457 1106 82-83 0.18539073176683019 1106 84-85 0.22947371641866354 1106 86-87 0.34033388602952286 1106 88-89 0.5265207305428383 1106 90-91 0.42515823431723376 1106 92-93 0.4666703655393398 1106 94-95 0.36342974855421417 1106 96-97 0.564413480417187 1106 98-99 0.4810585703192167 1106 100-101 0.08028857514825205 1107 1 -0.06733647087547467 1107 2 -0.006644916634442666 1107 3 0.014303559232963892 1107 4 -0.022478506911163265 1107 5 0.011477888837177375 1107 6 0.026926064726787047 1107 7 0.00423278628423418 1107 8 0.033348067471479226 1107 9 0.07193137222409263 1107 10-11 0.03663885149413204 1107 12-13 -0.040152610404241784 1107 14-15 0.006398919870569841 1107 16-17 0.06623529342133594 1107 18-19 0.028138723585840353 1107 20-21 0.1084150264144057 1107 22-23 0.12185791362654896 1107 24-25 0.08692306860235277 1107 26-27 0.1535904679240403 1107 28-29 -0.06754451025376795 1107 30-31 0.06787275250589886 1107 32-33 -0.033320807635909944 1107 34-35 -0.12384131776346408 1107 36-37 -0.021249703797678876 1107 38-39 -0.0824477688894234 1107 40-41 0.08944822420774301 1107 42-43 -0.0033700380729086987 1107 44-45 -0.02868449178943422 1107 46-47 -0.048390285070482264 1107 48-49 -0.05328830842886134 1107 50-51 -0.10138675899571581 1107 52-53 -0.053526082617018744 1107 54-55 -0.11481508905319515 1107 56-57 -0.20823969634714246 1107 58-59 -0.21517029244093777 1107 60-61 -0.0806868712546418 1107 62-63 0.03777510813853269 1107 64-65 0.040332284945854724 1107 66-67 0.08058823969148676 1107 68-69 0.06497067042846538 1107 70-71 0.04692031940853525 1107 72-73 0.04566987540689382 1107 74-75 -0.0033354457323611086 1107 76-77 0.04804581399849894 1107 78-79 0.32243549344153166 1107 80-81 0.14955318979830423 1107 82-83 0.14117276393657008 1107 84-85 0.2520860820584758 1107 86-87 0.10738299774316218 1107 88-89 0.22406647887449083 1107 90-91 0.373656968671753 1107 92-93 0.21421869852249742 1107 94-95 0.1703321020878512 1107 96-97 0.09384140532555563 1107 98-99 0.31974921921682053 1107 100-101 -0.09139565047348341 1108 1 0.008989643530647129 1108 2 0.0052776654498245534 1108 3 0.08103807345904102 1108 4 0.003308079984833512 1108 5 0.08564071501331938 1108 6 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2316 84-85 -0.32351627306028874 2316 86-87 -0.4044461160324566 2316 88-89 -0.7968924484652469 2316 90-91 -0.7404224790022624 2316 92-93 -0.8432624898130392 2316 94-95 -0.46539301218327367 2316 96-97 -0.6844628358005025 2316 98-99 -0.7189191019582637 2316 100-101 -0.30507859943528715 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 10.0 5 33.0 6 31.0 7 133.0 8 276.0 9 539.0 10 995.0 11 1595.0 12 2484.0 13 3677.0 14 4416.0 15 5967.0 16 7862.0 17 11789.0 18 11731.0 19 7689.0 20 6896.0 21 7199.0 22 7725.0 23 8390.0 24 9575.0 25 11099.0 26 13741.0 27 16289.0 28 19918.0 29 24575.0 30 29936.0 31 37418.0 32 47046.0 33 61981.0 34 85955.0 35 127786.0 36 213879.0 37 392643.0 38 400340.0 39 45233.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63121791494817 19.435167375863774 14.818251019997755 23.115363689190307 2 15.378530038558965 20.450327901803416 40.57204711656019 23.59909494307743 3 19.400206164148216 24.540049260568985 29.70048418455441 26.35926039072839 4 13.271094790636434 16.849339739952878 36.52750484215289 33.3520606272578 5 14.049097063728325 37.235494901524966 33.938896978779354 14.77651105596736 6 32.16670897723773 35.68434633855792 17.520080105282634 14.628864578921712 7 28.75996014405674 32.59741624811984 20.52482728988806 18.11779631793536 8 25.952710099646005 35.929419820648256 19.611779508857254 18.50609057084849 9 26.334181388798903 17.388845622146725 20.39629740499945 35.88067558405491 10-11 25.141914572805636 26.82334486270783 26.89741397379859 21.137326590687948 12-13 25.794122293327206 24.00942552489183 27.76122577310019 22.435226408680776 14-15 22.703209073046803 25.46798533722267 26.090449555441975 25.73835603428855 16-17 22.20394539696004 28.71841446491879 26.717347975283257 22.360292162837908 18-19 22.128452513987085 27.269312994742013 29.059297123657995 21.542937367612907 20-21 22.934194051236155 26.41215372690565 29.024027282020135 21.629624939838056 22-23 22.548512194357347 26.38882656702271 28.85382199686635 22.20883924175359 24-25 22.356277822103273 26.498567173206695 29.06081307849383 22.084341926196206 26-27 22.263809384772603 26.73911807417766 28.890623679377935 22.1064488616718 28-29 22.05215694783629 26.857582617792936 29.040900286994752 22.049360147376017 30-31 22.254610188255562 26.574520785994878 28.96266883943338 22.20820018631618 32-33 22.053816587669857 26.782837357140355 28.80910392357297 22.354242131616815 34-35 22.512880442567152 26.774261368631162 28.69718342328076 22.015674765520927 36-37 22.47905489075833 26.569884050236293 28.775807092254396 22.175253966750986 38-39 22.228083474894884 26.843481805421575 28.82451348016386 22.103921239519682 40-41 22.49289630386483 26.694220769719752 28.716207493093034 22.096675433322392 42-43 22.27005633845243 26.7231577811705 28.508689289546574 22.498096590830503 44-45 22.385776598648157 26.898344738052092 28.441823640346886 22.27405502295287 46-47 22.409613226115383 27.082746818683066 28.024479328662526 22.483160626539025 48-49 22.533618711532185 26.99061436017032 28.032875863556928 22.442891064740568 50-51 22.42196307237632 27.25723449741772 27.891586112617084 22.429216317588875 52-53 22.651400015059707 27.270581473792266 27.656448090874587 22.42157042027344 54-55 22.38144178444475 27.11264467295568 27.816977377879702 22.68893616471987 56-57 22.39650147923084 27.482344812918637 27.628177979775753 22.492975728074775 58-59 22.47290366046467 27.14597722628886 27.773041109228735 22.608078004017734 60-61 22.50942097142049 27.073351406844576 28.007856444682144 22.40937117705279 62-63 22.439053326456808 27.14297657166778 27.92970947097691 22.4882606308985 64-65 22.284886733639492 26.997889759771926 27.886919891396083 22.830303615192502 66-67 22.18734449864442 27.27197565519185 27.744745879089216 22.795933967074514 68-69 22.251075651684022 27.124248795721922 27.855037283213807 22.76963826938025 70-71 22.212848324807695 27.044800468141656 27.783494318496142 22.9588568885545 72-73 22.2853978125947 27.051453137610576 27.710422353750385 22.95272669604434 74-75 22.21363375682834 27.23207727283065 27.52872252604559 23.025566444295418 76-77 22.2096450432619 27.106459300300216 27.642339527773647 23.041556128664233 78-79 22.20670485496213 27.14532553995418 27.613991693154443 23.03397791192924 80-81 22.110373913553946 27.20172927757542 27.624367126637374 23.06352968223326 82-83 22.29071851649689 27.15803281429411 27.503946271695273 23.047302397513725 84-85 22.230168687022047 26.997637779303552 27.533486307049348 23.23870722662505 86-87 22.108788176303193 26.941086737200827 27.694897867822142 23.25522721867384 88-89 22.134893078205224 26.86384440736351 27.594226442193598 23.407036072237673 90-91 21.962120092408682 26.984921413431966 27.69329129871721 23.359667195442142 92-93 22.040447266788334 26.883768046278135 27.6295302788994 23.446254408034132 94-95 21.874298879005494 26.77911853455032 27.631620195726857 23.714962390717325 96-97 21.883181025522187 26.615060063594942 27.685958033240745 23.815800877642133 98-99 21.73212296752715 26.62597827931027 27.5600921051661 24.08180664799648 100-101 21.917676440776006 26.16124790930197 28.01446231334316 23.90661333657887 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 228.0 1 157.0 2 128.5 3 175.0 4 152.5 5 142.5 6 281.0 7 639.0 8 1026.5 9 1262.0 10 1325.0 11 1336.5 12 1437.0 13 1568.0 14 1700.0 15 1821.0 16 1910.5 17 2021.0 18 2128.5 19 2278.0 20 2563.0 21 2959.0 22 3540.0 23 4457.0 24 5528.5 25 6780.0 26 8654.0 27 10849.5 28 12868.0 29 15205.0 30 18062.0 31 21179.0 32 24367.5 33 27230.0 34 30354.5 35 33334.0 36 36954.0 37 40913.0 38 43364.0 39 45117.0 40 46985.5 41 50014.0 42 53260.5 43 55813.0 44 58441.0 45 60872.0 46 62199.0 47 62940.5 48 66681.5 49 67642.5 50 64783.5 51 62933.5 52 60452.0 53 58650.0 54 56494.0 55 52025.5 56 46369.0 57 40986.5 58 36407.5 59 31878.5 60 26389.5 61 21259.0 62 17269.0 63 13350.5 64 9701.0 65 7013.0 66 5049.5 67 3921.0 68 2979.5 69 2109.0 70 1614.0 71 1204.0 72 881.0 73 741.5 74 592.5 75 426.5 76 292.5 77 153.5 78 90.5 79 51.0 80 24.0 81 11.0 82 7.0 83 4.5 84 3.5 85 3.5 86 2.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35510145624175177 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011371606267045885 16-17 0.0016289057625768428 18-19 6.14681419840318E-5 20-21 0.0 22-23 0.0 24-25 3.0734070992015903E-4 26-27 0.001321565052656684 28-29 0.0 30-31 0.0033500137381297333 32-33 0.0 34-35 0.010326647853317343 36-37 0.02858268602257479 38-39 0.022005594830283387 40-41 0.014813822218151664 42-43 0.010603254492245487 44-45 0.002889002673249495 46-47 0.0010449584137285407 48-49 7.376177038083817E-4 50-51 6.14681419840318E-5 52-53 9.220221297604771E-5 54-55 0.0 56-57 0.0 58-59 0.0043642380808662585 60-61 0.010388115995301376 62-63 0.004179833654914163 64-65 0.0018440442595209542 66-67 0.003964695157970051 68-69 0.0033500137381297333 70-71 6.14681419840318E-5 72-73 0.0 74-75 0.0 76-77 0.21811970183033685 78-79 4.917451358722544E-4 80-81 0.0032270774541616697 82-83 4.302769938882226E-4 84-85 0.0 86-87 0.10962843122852073 88-89 0.4105764543823404 90-91 0.39637731358402906 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0014137672656327315 100-101 6.146814198403181E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1626859.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.40348757515876 #Duplication Level Percentage of deduplicated Percentage of total 1 79.87809102039904 51.444276425596705 2 12.327642195096422 15.878863018857906 3 3.6412775974733793 7.035329295197422 4 1.4547458152170976 3.7476281614139415 5 0.7477688063756446 2.407945951525256 6 0.4522889848130391 1.7477392808272638 7 0.29864812413695513 1.3463786526539203 8 0.20556777886468536 1.0591425513571797 9 0.14415113877571598 0.835545246757813 >10 0.7789088358967882 8.979850624992505 >50 0.047447124795138435 2.0697116347593227 >100 0.022413577474377964 2.4241885602707933 >500 6.67545888339347E-4 0.24616153081235703 >1k 3.8145479333676974E-4 0.7772390649776393 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4873 0.299534255888187 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4071 0.25023680601699344 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2501 0.15373182310206354 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.14681419840318E-5 0.0 0.0 0.0 0.0 6 1.229362839680636E-4 0.0 0.0 0.0 0.0 7 1.229362839680636E-4 0.0 0.0 0.0 0.0 8 1.229362839680636E-4 0.0 0.0 0.0 0.0 9 1.229362839680636E-4 0.0 0.0 0.0 0.0 10-11 1.229362839680636E-4 1.229362839680636E-4 0.0 0.0 0.0 12-13 1.229362839680636E-4 1.229362839680636E-4 0.0 0.0 0.0 14-15 1.229362839680636E-4 1.229362839680636E-4 0.0 3.07340709920159E-5 0.0 16-17 1.229362839680636E-4 1.229362839680636E-4 0.0 1.229362839680636E-4 0.0 18-19 1.229362839680636E-4 1.229362839680636E-4 0.0 1.229362839680636E-4 0.0 20-21 1.229362839680636E-4 1.229362839680636E-4 0.0 2.1513849694411132E-4 0.0 22-23 1.8440442595209542E-4 1.229362839680636E-4 0.0 3.6880885190419084E-4 0.0 24-25 2.458725679361272E-4 1.229362839680636E-4 0.0 7.376177038083817E-4 0.0 26-27 2.458725679361272E-4 1.229362839680636E-4 0.0 9.83490271744509E-4 0.0 28-29 2.458725679361272E-4 1.229362839680636E-4 0.0 0.0033192796671377175 0.0 30-31 3.0734070992015903E-4 1.229362839680636E-4 0.0 0.010080775285381215 0.0 32-33 3.0734070992015903E-4 1.229362839680636E-4 0.0 0.022712478463099754 0.0 34-35 3.3807478091217493E-4 1.229362839680636E-4 0.0 0.04118365512930131 0.0 36-37 3.6880885190419084E-4 1.229362839680636E-4 0.0 0.07059616106866053 0.0 38-39 4.3027699388822264E-4 2.458725679361272E-4 0.0 0.1151912980780756 0.0 40-41 4.3027699388822264E-4 2.458725679361272E-4 0.0 0.16734701655152662 0.0 42-43 4.6101106488023855E-4 2.458725679361272E-4 0.0 0.22002521423184185 0.0 44-45 4.917451358722544E-4 2.7660663892814313E-4 0.0 0.2856117217288038 0.0 46-47 6.45415490832334E-4 3.0734070992015903E-4 0.0 0.3655510403790372 0.0 48-49 7.990858457924135E-4 3.0734070992015903E-4 0.0 0.45268213164140225 0.0 50-51 7.990858457924135E-4 3.0734070992015903E-4 0.0 0.5467591229479629 0.0 52-53 8.298199167844294E-4 3.0734070992015903E-4 0.0 0.6429567651529726 0.0 54-55 9.220221297604771E-4 3.0734070992015903E-4 0.0 0.7496961937082439 0.0 56-57 9.220221297604771E-4 3.0734070992015903E-4 0.0 0.8806540702052237 0.0 58-59 9.220221297604771E-4 3.0734070992015903E-4 0.0 1.0260876941394428 0.0 60-61 9.220221297604771E-4 3.0734070992015903E-4 0.0 1.1879333119833988 0.0 62-63 9.83490271744509E-4 3.0734070992015903E-4 0.0 1.3514078355899313 0.0 64-65 9.83490271744509E-4 3.0734070992015903E-4 0.0 1.517187414520865 0.0 66-67 9.83490271744509E-4 3.0734070992015903E-4 0.0 1.7137625325857988 0.0 68-69 9.83490271744509E-4 3.0734070992015903E-4 0.0 1.9407951150038203 0.0 70-71 0.0010449584137285407 3.0734070992015903E-4 0.0 2.195119552462752 0.0 72-73 0.0010449584137285407 3.0734070992015903E-4 0.0 2.456389889965879 6.14681419840318E-5 74-75 0.0010449584137285407 3.0734070992015903E-4 0.0 2.7277717368253795 6.14681419840318E-5 76-77 0.0010449584137285407 3.0734070992015903E-4 0.0 3.0259844276609096 6.14681419840318E-5 78-79 0.0010449584137285407 3.0734070992015903E-4 0.0 3.363567463437213 6.14681419840318E-5 80-81 0.0011064265557125725 3.0734070992015903E-4 0.0 3.734404764026876 6.14681419840318E-5 82-83 0.0011678946976966043 3.0734070992015903E-4 0.0 4.120271025331636 6.14681419840318E-5 84-85 0.0011986287686886203 3.0734070992015903E-4 0.0 4.520551565931651 6.14681419840318E-5 86-87 0.0012293628396806361 3.0734070992015903E-4 0.0 4.944651011550478 6.14681419840318E-5 88-89 0.0012293628396806361 3.0734070992015903E-4 0.0 5.398285899392633 6.14681419840318E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6705 0.0 33.312023 1 GGTATCA 4525 0.0 32.310726 1 ATCAACG 8095 0.0 27.314379 3 TCAACGC 8240 0.0 26.77614 4 TATCAAC 8355 0.0 26.57796 2 CAACGCA 8375 0.0 26.344524 5 AACGCAG 8580 0.0 25.715082 6 GTACATG 6940 0.0 23.674812 1 TACATGG 7000 0.0 22.775267 2 ACGCAGA 9745 0.0 22.543505 7 CGCAGAG 9810 0.0 22.200663 8 GTGTAGC 990 0.0 22.12838 1 ACATGGG 6990 0.0 21.925402 3 GAGTACT 5065 0.0 20.423931 12-13 GCAGAGT 10495 0.0 20.345999 9 CATGGGG 3315 0.0 19.89544 4 AGTACTT 5300 0.0 19.652643 12-13 GTACTTT 5295 0.0 19.312727 14-15 AGAGTAC 9020 0.0 19.096878 10-11 GAGTACA 6165 0.0 18.848799 1 >>END_MODULE