FastQCFastQC Report
Fri 27 May 2016
SRR939160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605429
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47620.2966185362292571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37750.23513964180290753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23120.14401135148299923No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44450.034.188061
GTATCAA66350.032.9956171
ATCAACG79550.027.0333443
TCAACGC80850.026.5986714
TATCAAC81200.026.4840242
CAACGCA81100.026.3990445
AACGCAG83500.025.6849786
GTACATG70000.024.219571
TACATGG68850.023.5291522
ACGCAGA93900.022.7897917
CGCAGAG95000.022.4262358
ACATGGG70850.021.7283943
GAGTACT49150.020.85622612-13
CATGGGG31550.020.568634
GCAGAGT102250.020.1878769
ATCACGC1200.00485566319.7267828
ATAACGC1456.263862E-419.600513
GTACTTT51700.019.55400714-15
GTATAGG4700.019.1978231
AGTACTT50450.019.05180712-13
Produced by FastQC (version 0.11.5)