##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939160_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1605429 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13902452241737 33.0 31.0 34.0 30.0 34.0 2 32.24463305446706 34.0 31.0 34.0 30.0 34.0 3 32.31244234407127 34.0 31.0 34.0 30.0 34.0 4 35.738751448989646 37.0 35.0 37.0 35.0 37.0 5 35.77950815638686 37.0 35.0 37.0 35.0 37.0 6 35.85432367298709 37.0 37.0 37.0 35.0 37.0 7 35.786866937123975 37.0 36.0 37.0 35.0 37.0 8 35.77666218811296 37.0 36.0 37.0 35.0 37.0 9 37.46395885461145 39.0 38.0 39.0 35.0 39.0 10-11 37.37215597824631 39.0 38.0 39.0 35.0 39.0 12-13 37.277106306164896 39.0 37.0 39.0 34.0 39.0 14-15 38.588448321289825 40.0 38.0 41.0 34.0 41.0 16-17 38.50432719229564 40.0 38.0 41.0 34.0 41.0 18-19 38.39840970855765 40.0 38.0 41.0 34.0 41.0 20-21 38.37989596550206 40.0 38.0 41.0 34.0 41.0 22-23 38.42143003521177 40.0 38.0 41.0 34.0 41.0 24-25 38.383500298051175 40.0 38.0 41.0 34.0 41.0 26-27 38.28041975073329 40.0 38.0 41.0 34.0 41.0 28-29 38.14367997588184 40.0 38.0 41.0 33.5 41.0 30-31 37.943045441436524 40.0 38.0 41.0 33.0 41.0 32-33 37.76319725132659 40.0 38.0 41.0 33.0 41.0 34-35 37.469552063654014 40.0 37.5 41.0 32.5 41.0 36-37 37.225866419505316 40.0 37.0 41.0 31.0 41.0 38-39 37.04806783731949 40.0 37.0 41.0 31.0 41.0 40-41 36.870819263885224 40.0 37.0 41.0 30.5 41.0 42-43 36.67484921475817 40.0 36.0 41.0 30.0 41.0 44-45 36.40933638298548 40.0 36.0 41.0 30.0 41.0 46-47 36.28216227562851 40.0 36.0 41.0 29.5 41.0 48-49 36.176848991764814 40.0 36.0 41.0 29.0 41.0 50-51 35.07360369097606 38.5 34.5 40.0 26.5 40.5 52-53 35.21082339985138 38.5 35.0 40.0 27.5 40.5 54-55 35.74214773745834 39.0 35.0 41.0 28.0 41.0 56-57 35.66308911823569 39.0 35.0 41.0 28.0 41.0 58-59 35.46893852048269 39.0 35.0 41.0 27.0 41.0 60-61 35.193525531182004 39.0 35.0 41.0 26.5 41.0 62-63 34.821670718543146 38.0 35.0 40.0 26.0 41.0 64-65 34.45305553842618 37.5 34.0 40.0 25.5 41.0 66-67 34.07687353349167 37.0 34.0 40.0 24.5 41.0 68-69 33.67159151852869 36.5 34.0 39.0 23.5 41.0 70-71 33.25259292064614 36.0 33.5 39.0 22.5 41.0 72-73 32.80220551640714 35.5 33.0 39.0 21.5 40.0 74-75 32.35427103908052 35.0 33.0 37.5 20.0 40.0 76-77 31.911412775027735 35.0 32.0 37.0 19.0 39.0 78-79 31.48289024304407 35.0 32.0 36.5 17.5 39.0 80-81 31.037890183869855 35.0 31.5 36.0 13.5 38.5 82-83 30.642535110553005 35.0 31.0 36.0 7.5 37.0 84-85 30.26225793853232 35.0 31.0 35.0 4.5 37.0 86-87 29.855138097044467 34.0 30.5 35.0 2.0 36.5 88-89 29.44380754303055 34.0 30.0 35.0 2.0 36.0 90-91 29.04454510289773 34.0 29.5 35.0 2.0 36.0 92-93 28.645166494438556 34.0 29.0 35.0 2.0 35.0 94-95 28.174060951932475 34.0 29.0 35.0 2.0 35.0 96-97 27.59825504584756 34.0 28.0 35.0 2.0 35.0 98-99 26.906393867308985 34.0 26.5 35.0 2.0 35.0 100-101 23.99544701135958 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19998477476971743 1101 2 0.22782779942665599 1101 3 0.22346832538544703 1101 4 0.327604330695074 1101 5 0.2136942060419429 1101 6 0.18153093074589322 1101 7 0.21203080032746158 1101 8 0.2740810973272474 1101 9 0.2448082122651556 1101 10-11 0.3256354558920336 1101 12-13 0.3687677822296749 1101 14-15 0.5216472024514189 1101 16-17 0.5951023735410033 1101 18-19 0.5206817324177919 1101 20-21 0.6325529656272337 1101 22-23 0.6357134974963969 1101 24-25 0.48015589782989565 1101 26-27 0.484266189678884 1101 28-29 0.48608010813989466 1101 30-31 0.5453888373680442 1101 32-33 0.3574451163310357 1101 34-35 0.7471078775201789 1101 36-37 0.4968875390813281 1101 38-39 0.526148161666903 1101 40-41 0.539344034048554 1101 42-43 0.3686855010946175 1101 44-45 0.3740507867303151 1101 46-47 0.5988691249430289 1101 48-49 0.5814344887739296 1101 50-51 0.5217565571327683 1101 52-53 0.5598541674642021 1101 54-55 0.5291268705338581 1101 56-57 0.555891122269351 1101 58-59 0.44117781053601846 1101 60-61 0.26683344680206034 1101 62-63 0.26713703842550984 1101 64-65 0.28145959750849414 1101 66-67 0.3225216397446786 1101 68-69 0.421999541891104 1101 70-71 0.3633704697048401 1101 72-73 0.40419073756900303 1101 74-75 0.2532264094985237 1101 76-77 0.32713327602093045 1101 78-79 0.22509798659611846 1101 80-81 0.12503826357025716 1101 82-83 -0.04280131094275674 1101 84-85 -0.055069978122915586 1101 86-87 -0.03872995206682006 1101 88-89 0.019187385259719747 1101 90-91 -0.20577364745015814 1101 92-93 -0.124583912312346 1101 94-95 -0.08777469329348975 1101 96-97 -0.4793769722794572 1101 98-99 -0.5267360058702231 1101 100-101 -0.7705744029461208 1102 1 0.07073087038452996 1102 2 0.12356878012637651 1102 3 0.10502394156221584 1102 4 -0.03163320596181762 1102 5 0.027715893281936133 1102 6 0.09940244407876975 1102 7 0.0515418144446258 1102 8 0.16301924095406406 1102 9 0.1591674839012427 1102 10-11 0.16355232147206067 1102 12-13 0.1713746839551007 1102 14-15 0.11155036005900598 1102 16-17 0.24562579985212807 1102 18-19 0.10555522351784674 1102 20-21 0.267288354019783 1102 22-23 0.33206656303551085 1102 24-25 0.33409097250765996 1102 26-27 0.19327867007171307 1102 28-29 0.18937240848770642 1102 30-31 0.2774525351106618 1102 32-33 0.33860517907400123 1102 34-35 0.26044928105287823 1102 36-37 0.23083523054965127 1102 38-39 0.06997445915035883 1102 40-41 0.024280609101410278 1102 42-43 -0.05564785497166724 1102 44-45 -0.017581212178505723 1102 46-47 -0.056922663913276494 1102 48-49 0.016892785166213287 1102 50-51 0.0022346311967353927 1102 52-53 -0.025948533210971902 1102 54-55 0.0048139741279271675 1102 56-57 -0.18565221811119414 1102 58-59 -0.23139089982338135 1102 60-61 -0.23996086541236394 1102 62-63 -0.11994268605106129 1102 64-65 -0.175455767696441 1102 66-67 -0.09927130849238353 1102 68-69 -0.18521760330428805 1102 70-71 -0.19184503871771597 1102 72-73 -0.28102033792714565 1102 74-75 -0.3917984830882517 1102 76-77 -0.3631245156997309 1102 78-79 -0.41600547108648556 1102 80-81 -0.27274015830793985 1102 82-83 -0.6388508915197235 1102 84-85 -0.6806411436415658 1102 86-87 -0.5725736402615986 1102 88-89 -0.6565951967494179 1102 90-91 -0.5691798399073598 1102 92-93 -0.8138101961560373 1102 94-95 -0.8374081243785341 1102 96-97 -0.8683918396326611 1102 98-99 -0.8500242142859626 1102 100-101 -0.9307138696309742 1103 1 0.25578981186905025 1103 2 0.32928144603713605 1103 3 0.246218326899033 1103 4 0.2631118686213796 1103 5 0.3151962873583116 1103 6 0.16908926567077742 1103 7 0.31556879047430186 1103 8 0.3735615800671539 1103 9 0.34159616567316675 1103 10-11 0.36462814610420224 1103 12-13 0.41457930847880675 1103 14-15 0.5130522437337461 1103 16-17 0.5345485336870652 1103 18-19 0.47647240422367787 1103 20-21 0.44425561616147036 1103 22-23 0.4874699986172786 1103 24-25 0.5866814286990447 1103 26-27 0.647078077537131 1103 28-29 0.5942788379397328 1103 30-31 0.6690734635869617 1103 32-33 0.5170094021200171 1103 34-35 0.5798163297616341 1103 36-37 0.503044250673014 1103 38-39 0.5359905892583896 1103 40-41 0.4505125818715001 1103 42-43 0.5574597672950645 1103 44-45 0.5542845832572141 1103 46-47 0.5790125467118941 1103 48-49 0.6509787457112353 1103 50-51 0.599175031340458 1103 52-53 0.6950624009238808 1103 54-55 0.6250044716289196 1103 56-57 0.7721963908095333 1103 58-59 0.8282624760530979 1103 60-61 0.6844718806218069 1103 62-63 0.5948462584671361 1103 64-65 0.5922073137845913 1103 66-67 0.5992175562036124 1103 68-69 0.5422436528087786 1103 70-71 0.4740509277870828 1103 72-73 0.5004007768726666 1103 74-75 0.4244232806165229 1103 76-77 0.3012807013585501 1103 78-79 0.23850641940864747 1103 80-81 0.2575710299239873 1103 82-83 0.21004821838748455 1103 84-85 0.13822109959883022 1103 86-87 0.27725036362839006 1103 88-89 0.1128843238010333 1103 90-91 0.18283588571082987 1103 92-93 0.155641408141026 1103 94-95 -0.12402064032683668 1103 96-97 -0.13490411525375734 1103 98-99 -0.39619979514842996 1103 100-101 -0.40608920607830257 1104 1 0.3463322296644904 1104 2 0.35047261024121923 1104 3 0.3352700937527331 1104 4 0.3324532846413959 1104 5 0.32320199936289384 1104 6 0.32021662545820817 1104 7 0.4078969863517372 1104 8 0.3413057369381036 1104 9 0.41360252799299246 1104 10-11 0.38901703876128835 1104 12-13 0.4135349726158353 1104 14-15 0.5462048591556439 1104 16-17 0.5130997804439019 1104 18-19 0.4498133625377463 1104 20-21 0.5492657359157818 1104 22-23 0.560232559513139 1104 24-25 0.6175193671375965 1104 26-27 0.5126044316141005 1104 28-29 0.6907587478068606 1104 30-31 0.7560663303122013 1104 32-33 0.704700684633842 1104 34-35 0.6579395903884091 1104 36-37 0.6958264763562312 1104 38-39 0.38222624258444426 1104 40-41 0.41096269735940893 1104 42-43 0.5224879778252998 1104 44-45 0.5607439843769342 1104 46-47 0.860985818548329 1104 48-49 0.7242070379450922 1104 50-51 0.609237054638939 1104 52-53 0.6595780222105248 1104 54-55 0.6689344053392219 1104 56-57 0.735995163805498 1104 58-59 0.8312059809519496 1104 60-61 0.829130778368544 1104 62-63 0.7060004592913103 1104 64-65 0.7266519647604994 1104 66-67 0.8529960896909685 1104 68-69 0.7536859391432031 1104 70-71 0.6425524740979682 1104 72-73 0.5491984962129663 1104 74-75 0.567238928153948 1104 76-77 0.5617389457869848 1104 78-79 0.5777951940666455 1104 80-81 0.5764836054985452 1104 82-83 0.4905613875300219 1104 84-85 0.5192250599979431 1104 86-87 0.5926089871139872 1104 88-89 0.35827348000147197 1104 90-91 0.4912548792651492 1104 92-93 0.25308151674781243 1104 94-95 0.3853170270607329 1104 96-97 0.6080233181601251 1104 98-99 0.6190497348486552 1104 100-101 0.707351712315937 1105 1 -0.1312019664784927 1105 2 0.07764579763623658 1105 3 0.008889094223711425 1105 4 0.011213932455859776 1105 5 -0.0831145377218192 1105 6 0.09209972433021107 1105 7 -0.020495881449591025 1105 8 -2.188209358990889E-4 1105 9 0.04121253722698981 1105 10-11 0.05420405859707955 1105 12-13 0.04665553775064524 1105 14-15 0.050339343503743805 1105 16-17 -0.04397797223840172 1105 18-19 0.039396699005926905 1105 20-21 0.057711760656481204 1105 22-23 0.13409928797386783 1105 24-25 0.06289406638056505 1105 26-27 0.10889382846491458 1105 28-29 -0.08082014570822338 1105 30-31 0.19073540855242044 1105 32-33 0.06881618937648426 1105 34-35 0.05662315612661217 1105 36-37 -0.05267469156162008 1105 38-39 0.2645449143731966 1105 40-41 0.09497734536145686 1105 42-43 0.0960048575861947 1105 44-45 -0.05950189781609083 1105 46-47 -0.018206785825711336 1105 48-49 0.0985245821928018 1105 50-51 -0.11722514991446076 1105 52-53 0.09115890196338938 1105 54-55 0.1503400096395282 1105 56-57 0.06982104398219491 1105 58-59 0.03070952182204678 1105 60-61 0.1430676972623246 1105 62-63 0.2332447700492466 1105 64-65 0.11099374010597529 1105 66-67 0.10347647333580312 1105 68-69 0.16604269553665318 1105 70-71 0.01817862803440562 1105 72-73 0.1743384983898011 1105 74-75 0.171498354883596 1105 76-77 0.03088730621290736 1105 78-79 0.09160832420183951 1105 80-81 0.2155267705584123 1105 82-83 0.07463316494700223 1105 84-85 0.05838002616920335 1105 86-87 -0.20216500059441245 1105 88-89 0.006926324258863303 1105 90-91 0.23900092572405285 1105 92-93 0.20211796571579654 1105 94-95 0.17543689851704514 1105 96-97 0.1056792064773866 1105 98-99 0.31046236621746814 1105 100-101 -5.268804045037712E-4 1106 1 0.26708761605380715 1106 2 0.23242372299771574 1106 3 0.28139366737668325 1106 4 0.3264585524472352 1106 5 0.35587319341173895 1106 6 0.24536297482248415 1106 7 0.2900139231573462 1106 8 0.2259148549469785 1106 9 0.24999444734851295 1106 10-11 0.36803668119836175 1106 12-13 0.39248785159127664 1106 14-15 0.5096331846026985 1106 16-17 0.45725925497448117 1106 18-19 0.4105335498803626 1106 20-21 0.5533963936973691 1106 22-23 0.4522931547057851 1106 24-25 0.37775273556392364 1106 26-27 0.36243219634448565 1106 28-29 0.4364719069092544 1106 30-31 0.48890571043876463 1106 32-33 0.36456446789024 1106 34-35 0.2951291041383044 1106 36-37 0.29532331605835793 1106 38-39 0.3517377557867363 1106 40-41 0.5499514502468656 1106 42-43 0.47470124683432857 1106 44-45 0.4296128329795792 1106 46-47 0.4376338878550641 1106 48-49 0.4114170868824729 1106 50-51 0.4370184156301846 1106 52-53 0.4936619021847619 1106 54-55 0.5007396507780939 1106 56-57 0.48937141857293653 1106 58-59 0.5560031357197772 1106 60-61 0.5549959074991975 1106 62-63 0.4536685586006328 1106 64-65 0.2948461408656442 1106 66-67 0.16983234037064676 1106 68-69 0.20380616157881093 1106 70-71 0.1673624513068077 1106 72-73 0.22055210250039892 1106 74-75 0.2669949643421603 1106 76-77 0.3391083497073737 1106 78-79 0.31010745617662394 1106 80-81 0.3338753088971629 1106 82-83 0.3570858022289798 1106 84-85 0.4181131229802304 1106 86-87 0.12072435823206717 1106 88-89 0.09678004162053355 1106 90-91 0.08365936654104544 1106 92-93 0.08048374804536351 1106 94-95 0.2785658672564857 1106 96-97 0.41140540569208284 1106 98-99 0.30177337557350015 1106 100-101 0.07622489933846666 1107 1 0.15052520593013696 1107 2 0.2878630384995873 1107 3 0.1893135695912349 1107 4 0.16833013714668965 1107 5 0.28009666191907456 1107 6 0.2761330352193738 1107 7 0.2347471702094026 1107 8 0.2418373455998406 1107 9 0.19966370245349196 1107 10-11 0.22351913733870532 1107 12-13 0.2611030370960208 1107 14-15 0.2687592271681254 1107 16-17 0.3219001091591238 1107 18-19 0.40662213199137653 1107 20-21 0.35400216016270747 1107 22-23 0.3645522861409347 1107 24-25 0.5161281310197765 1107 26-27 0.4850810315472458 1107 28-29 0.4811853811686717 1107 30-31 0.5073375482299696 1107 32-33 0.6266096936595815 1107 34-35 0.734543469970923 1107 36-37 0.7280329076603138 1107 38-39 0.6202522798016972 1107 40-41 0.7826017234709113 1107 42-43 0.8220840480997822 1107 44-45 0.7816422405148131 1107 46-47 0.8179905171331754 1107 48-49 0.7646364570291482 1107 50-51 0.7501008142496843 1107 52-53 0.7887798199299496 1107 54-55 0.6210211434441817 1107 56-57 0.6913648755761059 1107 58-59 0.7242156752653628 1107 60-61 0.6164299742233865 1107 62-63 0.6013443567024481 1107 64-65 0.5994352262841076 1107 66-67 0.6038271634940315 1107 68-69 0.6788403145842707 1107 70-71 0.687676299451752 1107 72-73 0.6084475583531415 1107 74-75 0.7239244293982381 1107 76-77 0.7252620022814398 1107 78-79 0.5405377840454761 1107 80-81 0.6427155447326527 1107 82-83 0.6215307260138481 1107 84-85 0.8216344775962767 1107 86-87 0.6347432682119347 1107 88-89 0.5711077666708633 1107 90-91 0.5524650898657519 1107 92-93 0.7542452522156289 1107 94-95 0.8391353236844381 1107 96-97 0.808234568763698 1107 98-99 0.5889372380986657 1107 100-101 0.5203433922921654 1108 1 -0.03709828240234003 1108 2 0.03558702078539966 1108 3 0.0862029410782057 1108 4 0.06642884264830684 1108 5 0.025595725111031697 1108 6 0.12273710607612998 1108 7 -0.025640753483322953 1108 8 0.11225763175211512 1108 9 0.09075125142300067 1108 10-11 -0.015418011358271144 1108 12-13 -0.019764787806039408 1108 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-1.6436890471436278 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5117.0 3 1373.0 4 1406.0 5 1585.0 6 1696.0 7 2121.0 8 2529.0 9 3114.0 10 3728.0 11 4621.0 12 6047.0 13 9514.0 14 7943.0 15 7892.0 16 9615.0 17 13738.0 18 11613.0 19 8145.0 20 8168.0 21 8607.0 22 9261.0 23 10167.0 24 11300.0 25 12931.0 26 14968.0 27 17335.0 28 20352.0 29 24137.0 30 29245.0 31 35800.0 32 45182.0 33 58862.0 34 81737.0 35 122394.0 36 203233.0 37 349509.0 38 370120.0 39 70294.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.42379357679115 19.00159157497131 14.438117751532948 23.136497096704595 2 15.083890514194625 20.010926378535405 41.328527888770466 23.576655218499507 3 19.406295157297855 24.56170330598975 29.542422679480083 26.48957885723231 4 12.976240300238077 16.45957832193261 36.93759157117899 33.62658980665032 5 14.043620990020703 37.07416142149014 34.001094048910346 14.88112353957881 6 32.094450335278864 35.405161617691704 17.79667831742808 14.70370972960135 7 28.78406357016078 32.49286325694831 20.670339515081768 18.052733657809146 8 25.861084408403062 35.77435567508394 19.781968752220898 18.5825911642921 9 26.430892783334198 17.479431763703275 20.536753507213923 35.55292194574861 10-11 25.107177611552306 26.659219144785933 27.079077346795394 21.154525896866367 12-13 25.794120837536266 23.987243444357304 27.8356871844682 22.38294853363823 14-15 22.85376679974828 25.39722663697707 26.134157360876003 25.61484920239865 16-17 22.262694206040358 28.58999674277762 26.758907879828755 22.388401171353276 18-19 22.133736418778263 27.195549018959937 29.07558226166347 21.595132300598333 20-21 23.067878349956935 26.345009287750052 28.98083503403534 21.606277328257672 22-23 22.69949972893021 26.384770709872395 28.73654666125878 22.179182899938617 24-25 22.35683609181597 26.519815739471024 28.986704282055552 22.13664388665745 26-27 22.265081173037462 26.657158776874624 28.8972396459049 22.180520404183014 28-29 22.105196863355943 26.81689742116377 28.976762008332667 22.101143707147617 30-31 22.331571407862107 26.616089897168187 28.892356277853203 22.1599824171165 32-33 22.095254424864976 26.71373013584394 28.830682181296712 22.360333257994373 34-35 22.564374054241902 26.809120780145346 28.5830051371736 22.04350002843915 36-37 22.579150255147653 26.552139078875804 28.646832878210166 22.221877787766378 38-39 22.323864909980475 26.7940351813101 28.770494985279548 22.11160492342987 40-41 22.534779265238825 26.645913919499332 28.6492446665638 22.170062148698044 42-43 22.36165180381347 26.696595866577127 28.485366468338757 22.456385861270643 44-45 22.388931696010506 26.911972868390592 28.38784274963031 22.31125268596859 46-47 22.469480351959042 26.9755429541817 28.0528727542323 22.502103939626952 48-49 22.538885655179886 27.004900452033905 27.986431155239842 22.469782737546367 50-51 22.48765026412169 27.264697492918295 27.815891637310248 22.43176060564977 52-53 22.675982472591897 27.266860893810147 27.643803491392067 22.413353142205892 54-55 22.39835742581976 27.104947353030408 27.83741195631449 22.65928326483534 56-57 22.412929117902706 27.443952027248454 27.68996934799463 22.45314950685421 58-59 22.47666511434595 27.096978733206146 27.85608234310294 22.57027380934497 60-61 22.567415176762974 27.001950390938422 28.055161497687525 22.37547293461107 62-63 22.471763792542134 27.09210888783151 28.004401342315493 22.43172597731086 64-65 22.32999494309811 26.890506332086588 28.019588840998793 22.759909883816505 66-67 22.251328001182465 27.20201745323773 27.76794653110089 22.778708014478916 68-69 22.279296987554844 27.059232895109382 27.9597342593915 22.70173585794428 70-71 22.246995007860896 26.981822851093952 27.849528033022033 22.921654108023127 72-73 22.301092239090135 26.997626384087383 27.797109073600883 22.904172303221603 74-75 22.304794646025055 27.164107499128644 27.617464239870404 22.913633614975897 76-77 22.273334652453563 26.979400434808742 27.762481966457614 22.98478294628008 78-79 22.28532992205368 27.072034695393366 27.680725257919597 22.961910124633363 80-81 22.18279151852763 27.160231813722636 27.65449813656275 23.002478531186984 82-83 22.352638423551554 27.08540869784091 27.630863015611336 22.931089862996203 84-85 22.36309348279461 26.911137782736443 27.620659365152704 23.105109369316235 86-87 22.2261075362396 26.840201417183067 27.831697379604396 23.101993666972938 88-89 22.304173493185274 26.736774347464138 27.68698190598174 23.272070253368852 90-91 22.155259794977233 26.862228546180848 27.797722811248725 23.18478884759319 92-93 22.218690529911466 26.681712719760426 27.763945026269006 23.335651724059105 94-95 22.127636522028308 26.64173862886607 27.812569380098505 23.41805546900712 96-97 22.18934549921698 26.442070876564276 27.841765435141298 23.526818189077446 98-99 22.059361864497273 26.425903199633495 27.70695281545013 23.807782120419102 100-101 22.305266710455214 25.934759237077422 28.0677916858946 23.692182366572766 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1439.0 1 812.5 2 198.0 3 217.0 4 214.0 5 236.0 6 388.5 7 754.0 8 1187.0 9 1454.5 10 1541.0 11 1631.0 12 1802.0 13 1980.0 14 2145.5 15 2319.0 16 2467.5 17 2546.5 18 2577.5 19 2700.0 20 2894.5 21 3165.5 22 3720.5 23 4508.5 24 5366.0 25 6755.5 26 8699.5 27 10801.5 28 12712.5 29 14899.0 30 17751.5 31 20908.0 32 24036.5 33 26789.5 34 29801.0 35 32695.5 36 36061.0 37 39999.5 38 42648.5 39 44320.5 40 46305.5 41 49477.5 42 52720.5 43 54901.0 44 56999.0 45 59505.0 46 60730.5 47 61231.0 48 64740.0 49 65708.0 50 63150.0 51 61329.5 52 59049.0 53 57666.0 54 55463.5 55 50688.0 56 45200.5 57 40278.5 58 35986.0 59 31281.5 60 26003.0 61 21434.5 62 17374.5 63 13290.0 64 9689.5 65 7076.5 66 5267.5 67 4028.5 68 3067.0 69 2280.5 70 1762.5 71 1353.5 72 1027.5 73 799.0 74 625.0 75 487.0 76 352.5 77 205.0 78 124.0 79 91.5 80 62.0 81 39.5 82 27.5 83 28.0 84 21.0 85 13.5 86 12.0 87 9.5 88 6.5 89 6.5 90 7.0 91 5.5 92 3.0 93 1.5 94 2.0 95 1.5 96 0.5 97 2.5 98 3.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39765072139596336 2 0.12270863426535836 3 0.12495102555142582 4 0.1350417863387294 5 0.1376579095058081 6 0.07736249936932744 7 0.08757783745030144 8 0.08390280728702422 9 0.08583375533891564 10-11 0.0819718592351328 12-13 0.08664350774777334 14-15 0.0794491690383069 16-17 0.08106867385602229 18-19 0.0914397335540843 20-21 0.08935306388510486 22-23 0.09956840196607886 24-25 0.11398822370842934 26-27 0.11514056367488068 28-29 0.10841338981667828 30-31 0.09944382467240843 32-33 0.09757516526735222 34-35 0.34395790782401464 36-37 0.5362118162808819 38-39 0.518926716784112 40-41 0.49142627920636783 42-43 0.5186464178733535 44-45 0.5277405603112938 46-47 0.5247818495866214 48-49 0.5316647450619119 50-51 0.5314778791214062 52-53 0.5295469310695147 54-55 0.5246884166163686 56-57 0.4970945460683718 58-59 0.4871595068981562 60-61 0.43798262022176004 62-63 0.5100817289335124 64-65 0.5357757957530355 66-67 0.5458042678935038 68-69 0.5596634918143374 70-71 0.5555524411232138 72-73 0.5424718252878203 74-75 0.5471123294770432 76-77 0.5235360766499173 78-79 0.4972814120088774 80-81 0.5052855031272016 82-83 0.5182415416689247 84-85 0.5123864088664152 86-87 0.5136633261265369 88-89 0.5238163755606756 90-91 0.5406343102061817 92-93 0.5466140203023616 94-95 0.5418800831428858 96-97 0.5433750106669307 98-99 0.5438110311947773 100-101 0.5207330875423329 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1605429.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.70017981312746 #Duplication Level Percentage of deduplicated Percentage of total 1 80.85944684720569 53.124801974514355 2 11.780717147657043 15.479904698573236 3 3.393577209931818 6.688758987067556 4 1.4094378551506326 3.70401282075301 5 0.7285387553506061 2.3932563613683446 6 0.41694338053555136 1.6435953043847369 7 0.2751558389203411 1.2654451665588842 8 0.19659677640128576 1.033315484819655 9 0.13571335886760766 0.8024752872580787 >10 0.7377860427107076 8.634099711665092 >50 0.04500464795876562 2.0106957390966205 >100 0.02003766454251728 2.225365458945994 >500 6.646657611945301E-4 0.2495975839492365 >1k 3.7980900639687434E-4 0.7446754210452479 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4762 0.2966185362292571 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3775 0.23513964180290753 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2312 0.14401135148299923 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 6.228864683520729E-5 0.0 0.0 0.0 0.0 14-15 6.228864683520729E-5 0.0 0.0 0.0 0.0 16-17 6.228864683520729E-5 0.0 0.0 6.228864683520729E-5 0.0 18-19 6.228864683520729E-5 0.0 0.0 6.228864683520729E-5 0.0 20-21 6.228864683520729E-5 6.228864683520729E-5 0.0 6.228864683520729E-5 0.0 22-23 6.228864683520729E-5 6.228864683520729E-5 0.0 6.228864683520729E-5 0.0 24-25 6.228864683520729E-5 6.228864683520729E-5 0.0 4.983091746816583E-4 0.0 26-27 6.228864683520729E-5 6.228864683520729E-5 0.0 8.720410556929019E-4 0.0 28-29 6.228864683520729E-5 6.228864683520729E-5 0.0 0.0029898550480899496 0.0 30-31 6.228864683520729E-5 6.228864683520729E-5 0.0 0.008938420820852246 0.0 32-33 1.2457729367041458E-4 6.228864683520729E-5 0.0 0.021240428570805686 0.0 34-35 1.8686594050562185E-4 6.228864683520729E-5 0.0 0.03865010536124612 6.228864683520729E-5 36-37 3.114432341760364E-4 6.228864683520729E-5 0.0 0.070168160659861 6.228864683520729E-5 38-39 3.114432341760364E-4 6.228864683520729E-5 0.0 0.11654205822867283 6.228864683520729E-5 40-41 3.114432341760364E-4 6.228864683520729E-5 0.0 0.1674630270164548 6.228864683520729E-5 42-43 3.425875575936401E-4 6.228864683520729E-5 0.0 0.22112469626498588 6.228864683520729E-5 44-45 4.36020527846451E-4 6.228864683520729E-5 0.0 0.28335105445335795 6.228864683520729E-5 46-47 4.36020527846451E-4 6.228864683520729E-5 0.0 0.3614610175847079 6.228864683520729E-5 48-49 4.36020527846451E-4 6.228864683520729E-5 0.0 0.4482291026261516 6.228864683520729E-5 50-51 4.36020527846451E-4 6.228864683520729E-5 0.0 0.5390770940353015 6.228864683520729E-5 52-53 4.6716485126405464E-4 6.228864683520729E-5 0.0 0.6375243003583466 6.228864683520729E-5 54-55 4.983091746816583E-4 6.228864683520729E-5 0.0 0.7457196799111017 6.228864683520729E-5 56-57 5.605978215168656E-4 6.228864683520729E-5 0.0 0.8732245399827709 6.228864683520729E-5 58-59 5.605978215168656E-4 6.228864683520729E-5 0.0 1.0183259427853864 6.228864683520729E-5 60-61 5.605978215168656E-4 6.228864683520729E-5 0.0 1.1773177138322528 6.228864683520729E-5 62-63 5.605978215168656E-4 6.228864683520729E-5 0.0 1.3436284008822563 6.228864683520729E-5 64-65 5.605978215168656E-4 6.228864683520729E-5 0.0 1.5194069622512112 6.228864683520729E-5 66-67 6.228864683520728E-4 6.228864683520729E-5 0.0 1.7182946115960283 6.228864683520729E-5 68-69 6.228864683520728E-4 6.228864683520729E-5 0.0 1.9435615028755553 6.228864683520729E-5 70-71 6.228864683520728E-4 6.228864683520729E-5 0.0 2.1980106251973774 6.228864683520729E-5 72-73 6.228864683520728E-4 6.228864683520729E-5 0.0 2.4594983646115773 6.228864683520729E-5 74-75 6.228864683520728E-4 6.228864683520729E-5 0.0 2.7279001438244856 6.228864683520729E-5 76-77 6.228864683520728E-4 6.228864683520729E-5 0.0 3.0178849391657927 6.228864683520729E-5 78-79 6.228864683520728E-4 6.228864683520729E-5 0.0 3.3513160656746575 6.228864683520729E-5 80-81 6.228864683520728E-4 9.343297025281094E-5 0.0 3.72090575167136 6.228864683520729E-5 82-83 6.540307917696765E-4 1.2457729367041458E-4 0.0 4.102953167035103 6.228864683520729E-5 84-85 7.474637620224875E-4 1.2457729367041458E-4 0.0 4.491104869788698 6.228864683520729E-5 86-87 8.097524088576947E-4 1.2457729367041458E-4 0.0 4.899095506559306 6.228864683520729E-5 88-89 8.097524088576947E-4 1.2457729367041458E-4 0.0 5.351124216642406 6.228864683520729E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4445 0.0 34.18806 1 GTATCAA 6635 0.0 32.995617 1 ATCAACG 7955 0.0 27.033344 3 TCAACGC 8085 0.0 26.598671 4 TATCAAC 8120 0.0 26.484024 2 CAACGCA 8110 0.0 26.399044 5 AACGCAG 8350 0.0 25.684978 6 GTACATG 7000 0.0 24.21957 1 TACATGG 6885 0.0 23.529152 2 ACGCAGA 9390 0.0 22.789791 7 CGCAGAG 9500 0.0 22.426235 8 ACATGGG 7085 0.0 21.728394 3 GAGTACT 4915 0.0 20.856226 12-13 CATGGGG 3155 0.0 20.56863 4 GCAGAGT 10225 0.0 20.187876 9 ATCACGC 120 0.004855663 19.726782 8 ATAACGC 145 6.263862E-4 19.60051 3 GTACTTT 5170 0.0 19.554007 14-15 GTATAGG 470 0.0 19.197823 1 AGTACTT 5045 0.0 19.051807 12-13 >>END_MODULE