FastQCFastQC Report
Fri 27 May 2016
SRR939160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605429
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48640.3029719782064482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39330.24498124800287024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25520.158960626723449No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46800.035.3377951
GTATCAA68200.030.7485141
ATCAACG83700.024.9466613
TCAACGC84850.024.664484
CAACGCA85450.024.4912935
TATCAAC85800.024.4466972
AACGCAG87700.023.9170676
GTACATG69750.023.232211
TACATGG68950.022.506022
ACATGGG71150.021.0097483
ACGCAGA101750.020.5212357
CGCAGAG102700.020.28528
GAGTACT54900.020.05461712-13
GTACTTT56350.019.24380914-15
GGACCGT1751.0011887E-418.9821416
GCAGAGT110250.018.7669249
AGTACTT56950.018.6244112-13
TCCGATA1300.0076055318.252062
AGAGTAC98150.017.88999210-11
CATGGGG32900.017.7416674
Produced by FastQC (version 0.11.5)