##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939160_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1605429 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.54837242880252 34.0 31.0 34.0 31.0 34.0 2 32.76385937964245 34.0 31.0 34.0 31.0 34.0 3 32.87274429451567 34.0 31.0 34.0 31.0 34.0 4 36.30755455395411 37.0 37.0 37.0 35.0 37.0 5 36.26633815634326 37.0 37.0 37.0 35.0 37.0 6 36.30409130519008 37.0 37.0 37.0 35.0 37.0 7 36.26033103924247 37.0 37.0 37.0 35.0 37.0 8 36.235825439804564 37.0 37.0 37.0 35.0 37.0 9 37.99558560359879 39.0 38.0 39.0 35.0 39.0 10-11 37.916121796728476 39.0 38.0 39.0 35.0 39.0 12-13 37.83399265866008 39.0 38.0 39.0 35.0 39.0 14-15 39.27348235269203 40.5 39.0 41.0 36.0 41.0 16-17 39.35874834701504 41.0 39.0 41.0 36.0 41.0 18-19 39.386448419705886 41.0 39.0 41.0 36.0 41.0 20-21 39.35331895711364 41.0 39.0 41.0 36.0 41.0 22-23 39.289555003678146 41.0 39.0 41.0 36.0 41.0 24-25 39.224107388118696 41.0 39.0 41.0 36.0 41.0 26-27 39.09019925515236 40.5 39.0 41.0 35.5 41.0 28-29 38.9461645454268 40.0 38.5 41.0 35.0 41.0 30-31 38.76865653977846 40.0 38.0 41.0 35.0 41.0 32-33 38.691679918576284 40.0 38.0 41.0 35.0 41.0 34-35 38.605256601195066 40.0 38.0 41.0 35.0 41.0 36-37 38.47107315240973 40.0 38.0 41.0 34.0 41.0 38-39 38.35393468038761 40.0 38.0 41.0 34.0 41.0 40-41 38.17423878601919 40.0 38.0 41.0 34.0 41.0 42-43 37.99669683305833 40.0 38.0 41.0 33.0 41.0 44-45 37.75499850818691 40.0 38.0 41.0 33.0 41.0 46-47 37.46022433879044 40.0 37.0 41.0 33.0 41.0 48-49 37.13474778392566 40.0 37.0 41.0 31.5 41.0 50-51 36.886644628943415 40.0 36.5 41.0 31.0 41.0 52-53 36.623153063760526 39.5 36.0 41.0 31.0 41.0 54-55 36.383255192225874 39.0 36.0 41.0 30.0 41.0 56-57 36.15060055598846 39.0 35.0 40.5 30.0 41.0 58-59 35.82004841073632 38.5 35.0 40.0 29.0 41.0 60-61 35.451076628116226 38.0 35.0 40.0 28.0 41.0 62-63 35.0610164012236 38.0 34.0 40.0 28.0 41.0 64-65 34.60796086279742 37.0 34.0 40.0 27.0 41.0 66-67 34.185773709083364 36.5 34.0 39.0 26.0 41.0 68-69 33.699161096504426 36.0 33.0 39.0 26.0 40.0 70-71 33.192515520773576 35.5 33.0 38.5 25.0 40.0 72-73 32.62241338607936 35.0 32.0 37.5 23.5 39.5 74-75 32.0682985669251 35.0 31.5 37.0 22.0 39.0 76-77 30.06141380279041 33.0 29.5 35.0 20.0 37.5 78-79 31.04455257753535 34.0 31.0 36.0 20.0 38.0 80-81 31.06104131668233 34.0 31.0 36.0 20.0 37.0 82-83 30.680131292009797 34.0 31.0 35.0 18.5 37.0 84-85 30.209735840077634 34.0 30.5 35.0 15.5 36.5 86-87 29.517612426335887 34.0 29.5 35.0 7.0 36.0 88-89 28.84465180334976 34.0 29.0 35.0 2.0 35.5 90-91 28.289675220766537 33.0 28.5 35.0 2.0 35.0 92-93 27.85283622010067 33.0 27.0 35.0 2.0 35.0 94-95 27.33255379091819 33.0 26.5 34.5 2.0 35.0 96-97 26.557864595693736 32.5 25.5 34.0 2.0 35.0 98-99 25.385219153260593 32.0 21.5 34.0 2.0 35.0 100-101 19.57859924045224 25.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12164148521512175 1101 2 0.13106867237979714 1101 3 0.18632040786616244 1101 4 0.11312263663153743 1101 5 0.1864806407658719 1101 6 0.15122559237556032 1101 7 0.14650439834738194 1101 8 0.18257082751353693 1101 9 0.1568139698193889 1101 10-11 0.14725174594311596 1101 12-13 0.19097546846110447 1101 14-15 0.2587182819028371 1101 16-17 0.24182316377449808 1101 18-19 0.23666042574653545 1101 20-21 0.3092654316109744 1101 22-23 0.20644635430355152 1101 24-25 0.21552212637103452 1101 26-27 0.27065501373035517 1101 28-29 0.2864174578227505 1101 30-31 0.2171089158909112 1101 32-33 0.1702522879433488 1101 34-35 0.11998660374775483 1101 36-37 0.2204598348798683 1101 38-39 0.21534689548809638 1101 40-41 0.3129855741502823 1101 42-43 0.3083185123610406 1101 44-45 0.33041051515365183 1101 46-47 0.40557954705071353 1101 48-49 0.258056260723059 1101 50-51 0.25616933540514 1101 52-53 0.17993497833497685 1101 54-55 0.07352923939338751 1101 56-57 0.12191798034887569 1101 58-59 0.09991767264704521 1101 60-61 0.13136624864766588 1101 62-63 0.07316836416493544 1101 64-65 -0.05264573397195704 1101 66-67 -0.021109537179057725 1101 68-69 0.11160057353334452 1101 70-71 0.01440165721319886 1101 72-73 0.045931794423587746 1101 74-75 -0.039196712181620796 1101 76-77 -0.06124755488750466 1101 78-79 0.08937151976476798 1101 80-81 0.10352029958173858 1101 82-83 0.20461288187783921 1101 84-85 0.11014060729969444 1101 86-87 0.4026128179357187 1101 88-89 0.23611625818385917 1101 90-91 0.5680990505095629 1101 92-93 0.7005100397822872 1101 94-95 0.4357693596579466 1101 96-97 0.3562902844453113 1101 98-99 0.09215630851338119 1101 100-101 -0.4105334926023332 1102 1 0.004816824504487727 1102 2 0.04653617561924506 1102 3 0.038738057733176845 1102 4 0.04741302741105358 1102 5 0.09596369334720123 1102 6 0.10462871588370604 1102 7 0.059090499418104514 1102 8 0.09345195688679553 1102 9 0.1543076614241059 1102 10-11 0.11515565373824899 1102 12-13 0.08067232449329254 1102 14-15 0.10954178323272146 1102 16-17 0.1366690343330461 1102 18-19 0.1376100949820227 1102 20-21 0.12599802384994518 1102 22-23 0.032402843425835215 1102 24-25 0.18785043572110283 1102 26-27 0.15526252924559714 1102 28-29 0.0028806645335208714 1102 30-31 -0.10452546997827739 1102 32-33 -0.07412130545500162 1102 34-35 0.06130147792084273 1102 36-37 -0.06896763562411934 1102 38-39 -0.01945328182895878 1102 40-41 -0.014258168603582533 1102 42-43 0.07370588193069949 1102 44-45 0.08009509538961623 1102 46-47 -0.1684844234873779 1102 48-49 -0.18447766821371658 1102 50-51 -0.14444786434933832 1102 52-53 -0.13316602418837675 1102 54-55 -0.15085356085211998 1102 56-57 -8.996558783636033E-4 1102 58-59 -0.12030499665664252 1102 60-61 -0.3023188771792391 1102 62-63 -0.27551539768462163 1102 64-65 -0.28667014916666034 1102 66-67 -0.1451888524624252 1102 68-69 -0.16630981315423554 1102 70-71 -0.2386928664177077 1102 72-73 -0.3677710631089752 1102 74-75 -0.33348335202340706 1102 76-77 -0.32073572238392245 1102 78-79 -0.21055857639563413 1102 80-81 -0.28686706998792744 1102 82-83 -0.22590952145026932 1102 84-85 -0.16366932245546906 1102 86-87 -0.13794845874708983 1102 88-89 -0.013900791533114187 1102 90-91 0.06661572513276681 1102 92-93 -0.11323885198611094 1102 94-95 -0.14010188087945963 1102 96-97 -0.22750907448533297 1102 98-99 -0.13046184602525202 1102 100-101 -0.982082629886655 1103 1 0.07493556809020419 1103 2 0.0808930690512355 1103 3 0.08197113639568698 1103 4 0.035214861815497045 1103 5 0.1659388215253017 1103 6 0.09686864290341646 1103 7 0.06447527037573764 1103 8 0.1347932925208397 1103 9 0.20359033742559518 1103 10-11 0.13023822990820122 1103 12-13 0.18040569468970347 1103 14-15 0.14139401319809508 1103 16-17 0.16896663342767226 1103 18-19 0.27264273386941795 1103 20-21 0.24522732010217396 1103 22-23 0.21057954093843279 1103 24-25 0.20358627373583005 1103 26-27 0.14593344448991985 1103 28-29 0.22113628059520352 1103 30-31 0.1956955471415398 1103 32-33 0.2521099680640404 1103 34-35 0.3186021967509234 1103 36-37 0.17749549878484316 1103 38-39 0.2592485532209423 1103 40-41 0.2247003884004357 1103 42-43 0.22051767367007358 1103 44-45 0.10530444039577702 1103 46-47 0.1659469132869944 1103 48-49 0.026073008123404406 1103 50-51 -0.11746699370648628 1103 52-53 -0.1581023923429825 1103 54-55 -0.23063245767063734 1103 56-57 -0.13649438503154698 1103 58-59 -0.06511230458313122 1103 60-61 -0.14187814462233206 1103 62-63 -0.12706924109111384 1103 64-65 -0.24532504833466362 1103 66-67 -0.3362546293948867 1103 68-69 -0.3155547295991141 1103 70-71 -0.26254028553587716 1103 72-73 -0.3679138716884651 1103 74-75 -0.4040751658375932 1103 76-77 -0.44671736327870804 1103 78-79 -0.4179093723584799 1103 80-81 -0.267178849893817 1103 82-83 -0.3759252277045846 1103 84-85 -0.5557245934098702 1103 86-87 -0.490513526746561 1103 88-89 -0.44940187483435423 1103 90-91 -0.27642554358833493 1103 92-93 -0.3884802011748718 1103 94-95 -0.3933824440670115 1103 96-97 -0.6523734735704956 1103 98-99 -0.5392316375193502 1103 100-101 -0.69289902162215 1104 1 0.15703424307289993 1104 2 0.15034166282218564 1104 3 0.12334330155712081 1104 4 0.13152261418414923 1104 5 0.07201642132357478 1104 6 0.0916293595135329 1104 7 0.10285163618013371 1104 8 0.0818381637547958 1104 9 0.19726374351898812 1104 10-11 0.20827476457709082 1104 12-13 0.16667166844617043 1104 14-15 0.19671663117258475 1104 16-17 0.25583187060892953 1104 18-19 0.2236208214559099 1104 20-21 0.2438490245804985 1104 22-23 0.37234183311326774 1104 24-25 0.21343886031811365 1104 26-27 0.19931598984241816 1104 28-29 0.283355294963755 1104 30-31 0.23374830862547924 1104 32-33 0.39438895019642217 1104 34-35 0.369243166300528 1104 36-37 0.38286696806753895 1104 38-39 0.3368333362584863 1104 40-41 0.3960260943367899 1104 42-43 0.37184489148580724 1104 44-45 0.39301120414029356 1104 46-47 0.45220341190895397 1104 48-49 0.3875263487814209 1104 50-51 0.5640411163355523 1104 52-53 0.48530842826062326 1104 54-55 0.3301963525372358 1104 56-57 0.4791480782756068 1104 58-59 0.3958806895127722 1104 60-61 0.560011342172281 1104 62-63 0.4897620150476669 1104 64-65 0.46828486917988954 1104 66-67 0.44718987712406033 1104 68-69 0.35437015795485394 1104 70-71 0.363786815782305 1104 72-73 0.44614308577853734 1104 74-75 0.2384870347960515 1104 76-77 0.20240313195498771 1104 78-79 0.3475318608380391 1104 80-81 0.5703966340499704 1104 82-83 0.46144729160941367 1104 84-85 0.2807066256567907 1104 86-87 0.2081686529071618 1104 88-89 0.2677948729681141 1104 90-91 0.6569179207175893 1104 92-93 0.431686923629222 1104 94-95 0.6208258566685778 1104 96-97 0.011220160174648441 1104 98-99 -0.06368628657434527 1104 100-101 -0.8115354721021504 1105 1 -0.08515435020428441 1105 2 0.053911323341047535 1105 3 0.04311083148486006 1105 4 -0.005236377429014283 1105 5 -0.015032957249836443 1105 6 0.027734698330085905 1105 7 0.0181551468554062 1105 8 -0.06502305389924601 1105 9 0.006895893285324917 1105 10-11 7.457697688977305E-4 1105 12-13 0.028239421976131496 1105 14-15 -0.022887034138328488 1105 16-17 0.10047146124331618 1105 18-19 0.13218867881192864 1105 20-21 0.15022700462683503 1105 22-23 0.11146605272495691 1105 24-25 0.022419213116073422 1105 26-27 0.0815371052553715 1105 28-29 0.036822809693767056 1105 30-31 0.07731277282530158 1105 32-33 0.12268842469608643 1105 34-35 0.24599385182842326 1105 36-37 0.19865294392472066 1105 38-39 0.1732006979579026 1105 40-41 0.26212984315996835 1105 42-43 0.3196246437979511 1105 44-45 0.25849274893170815 1105 46-47 0.3477300633578295 1105 48-49 0.3655119626016159 1105 50-51 0.2760767642879429 1105 52-53 0.2744356824022489 1105 54-55 0.3521751268780591 1105 56-57 0.2898405580082155 1105 58-59 0.07351981518694828 1105 60-61 0.2010422497374904 1105 62-63 -6.61660505407724E-4 1105 64-65 0.04616921496783988 1105 66-67 0.07774939930346392 1105 68-69 0.2424288562970034 1105 70-71 0.01800463684936915 1105 72-73 -0.1948002969148135 1105 74-75 -0.12821283614865564 1105 76-77 -0.23320339032824222 1105 78-79 -0.05482097825312593 1105 80-81 -0.02951589123568965 1105 82-83 0.03512141519453493 1105 84-85 -0.07485100307624393 1105 86-87 -0.2211140092934798 1105 88-89 -0.324289568219438 1105 90-91 -0.019520935510396242 1105 92-93 -0.06126995745582775 1105 94-95 -0.043072365698765935 1105 96-97 0.03857306196271537 1105 98-99 -0.31163547098799427 1105 100-101 -1.036639912597522 1106 1 -0.05473560085967932 1106 2 0.054839061698437774 1106 3 0.10385511941289849 1106 4 0.02050726967504346 1106 5 0.04841589917064226 1106 6 0.07573387108458007 1106 7 0.08508484263878557 1106 8 0.13114044942721392 1106 9 0.0817470473510653 1106 10-11 0.08697591878960509 1106 12-13 0.1587745391304054 1106 14-15 0.10547146610052494 1106 16-17 0.10479556671129586 1106 18-19 0.10861509474165132 1106 20-21 0.20902187686983353 1106 22-23 0.20386194795606372 1106 24-25 0.18696606317491415 1106 26-27 0.11568210042006655 1106 28-29 0.08297597245140764 1106 30-31 0.13480196620267293 1106 32-33 0.04007092088026809 1106 34-35 0.039383461158827515 1106 36-37 0.11994956386948985 1106 38-39 0.08774609159859637 1106 40-41 0.058794208685966964 1106 42-43 -0.059454783421927004 1106 44-45 0.05821413614086168 1106 46-47 0.08254617600302794 1106 48-49 -0.0533691878521978 1106 50-51 0.048521624297030996 1106 52-53 -0.002478196680087308 1106 54-55 0.05704509786620804 1106 56-57 0.018268092467060626 1106 58-59 -0.08223391033066463 1106 60-61 -0.014116587225238675 1106 62-63 0.16388955880785971 1106 64-65 0.34868692501246557 1106 66-67 0.22896783872229065 1106 68-69 0.11038561815515635 1106 70-71 0.20966999273970544 1106 72-73 0.11137156625179756 1106 74-75 0.13384193682488643 1106 76-77 -0.008373936892841272 1106 78-79 0.12374025539129718 1106 80-81 0.19468730239801602 1106 82-83 0.08327132722687836 1106 84-85 -0.11610550340830983 1106 86-87 -0.10166514704733132 1106 88-89 -0.14586960713493013 1106 90-91 0.10761635811515546 1106 92-93 0.13703156770525027 1106 94-95 -0.07921338192909033 1106 96-97 -0.27975115419303975 1106 98-99 -0.12477702318019723 1106 100-101 -1.147537415714261 1107 1 0.07836405401521773 1107 2 0.11348516466076575 1107 3 0.06336763019805858 1107 4 0.06232852362960273 1107 5 0.08499556554970411 1107 6 0.08687134591920653 1107 7 0.08477926311621076 1107 8 0.07481675083647588 1107 9 0.030704481921098647 1107 10-11 0.11484117619541934 1107 12-13 0.07237822046489129 1107 14-15 0.11534707725957105 1107 16-17 0.16240988940327128 1107 18-19 0.23845223883138544 1107 20-21 0.1579146004821439 1107 22-23 0.11769571269577739 1107 24-25 0.17773216901328226 1107 26-27 0.18363399860815122 1107 28-29 0.2635680777145666 1107 30-31 0.181070300974028 1107 32-33 0.2275870986197006 1107 34-35 0.16864539673547085 1107 36-37 0.2596749018044946 1107 38-39 0.14251133462418863 1107 40-41 0.21516284544178887 1107 42-43 0.0833784780103457 1107 44-45 0.06844206251417262 1107 46-47 0.33252460568379405 1107 48-49 0.21817416848947602 1107 50-51 0.19049387048627864 1107 52-53 0.08405113895408078 1107 54-55 0.06903280278424262 1107 56-57 0.12754512726717593 1107 58-59 0.14777939136620688 1107 60-61 0.04854159511586431 1107 62-63 0.025925796944186175 1107 64-65 0.02179952207890068 1107 66-67 0.11858352753448287 1107 68-69 -0.05667290931105384 1107 70-71 0.06533939164680191 1107 72-73 -0.07542560084628036 1107 74-75 -0.25927533381071655 1107 76-77 -0.16236444595237742 1107 78-79 -0.005568415877036159 1107 80-81 0.20868104700543455 1107 82-83 0.17181389265684643 1107 84-85 0.09286235681602406 1107 86-87 0.031265540326842256 1107 88-89 -0.046318988785778004 1107 90-91 0.28087814988966997 1107 92-93 0.32558851334804473 1107 94-95 0.22275311217502036 1107 96-97 -0.2731883845640475 1107 98-99 -0.1815123553631146 1107 100-101 -1.002498589139627 1108 1 -0.11525074755180498 1108 2 -0.15796135211661522 1108 3 -0.06917793273840545 1108 4 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sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 10.0 5 29.0 6 45.0 7 130.0 8 292.0 9 641.0 10 1044.0 11 1787.0 12 2556.0 13 3788.0 14 4887.0 15 6343.0 16 8470.0 17 12542.0 18 12651.0 19 8847.0 20 8189.0 21 8867.0 22 9363.0 23 10077.0 24 11524.0 25 13399.0 26 15888.0 27 19018.0 28 23321.0 29 27768.0 30 34386.0 31 42187.0 32 54026.0 33 70013.0 34 99000.0 35 149064.0 36 248053.0 37 403973.0 38 279392.0 39 13855.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73239228678815 19.400577039531964 14.77669120627787 23.09033946740202 2 15.380748697077228 20.47876299730477 40.56031129374142 23.580177011876575 3 19.361678404962163 24.537428936440044 29.707947221583765 26.39294543701403 4 13.227305598690444 16.837057260084375 36.56567808355275 33.36995905767243 5 14.026032917058306 37.23179287280845 33.94862058677151 14.79355362336173 6 32.16255592741878 35.64903835672584 17.516128087881803 14.672277627973582 7 28.715190768324227 32.5818830979134 20.557807290138648 18.145118843623727 8 25.851221075488233 35.903487479047655 19.661037641652168 18.58425380381194 9 26.361614247655922 17.30746112098386 20.475960008197188 35.854964623163035 10-11 25.13194292615868 26.81080882430802 26.86942244097995 21.187825808553352 12-13 25.768844340048673 23.99495711115222 27.82985108653201 22.406347462267096 14-15 22.728923388751955 25.48225788066278 26.045476035198334 25.74334269538693 16-17 22.197354598370055 28.722873794801824 26.77278629267228 22.306985314155845 18-19 22.076684798891762 27.279811190653714 29.026073404678748 21.617430605775777 20-21 22.931378466441057 26.361365093068585 29.095992410751272 21.61126402973909 22-23 22.5079091009319 26.355260805678732 28.945129308116396 22.19170078527297 24-25 22.357527089349382 26.526259664089576 29.027835567836114 22.088377678724928 26-27 22.22752387300124 26.761992562462233 28.8984782324324 22.112005332104125 28-29 22.045997094231907 26.824070224286057 29.078765624732355 22.051167056749684 30-31 22.260436307055624 26.588220344842405 28.95014354418663 22.20119980391534 32-33 22.07137974153302 26.748887757310747 28.807093437729204 22.37263906342703 34-35 22.45746715423958 26.774698952798044 28.719248954978415 22.048584937983964 36-37 22.492282760766987 26.537022654991976 28.80037233401277 22.17032225022827 38-39 22.254089206998025 26.845333604965894 28.821024360020296 22.079552828015782 40-41 22.46100618176257 26.70152648763943 28.753434355798795 22.084032974799204 42-43 22.310832420202544 26.706350219867776 28.521357293386902 22.461460066542784 44-45 22.316004803882972 26.91326848423539 28.463442649909553 22.307284061972084 46-47 22.42933339562553 27.06951030546536 28.0272528362416 22.473903462667508 48-49 22.493141793573592 27.065435137936532 27.985188516584277 22.456234551905595 50-51 22.44909654285975 27.239379936608348 27.857288124296716 22.454235396235184 52-53 22.664315893135107 27.295414496685932 27.605082504427163 22.435187105751798 54-55 22.37187069624381 27.156510814243422 27.786903064539135 22.684715424973636 56-57 22.41164897720453 27.415235247162983 27.689118512596483 22.483997263036006 58-59 22.40212234107825 27.18779217850787 27.833177712428764 22.576907767985116 60-61 22.485935603548935 27.064187466733024 28.04591550866195 22.40396142105609 62-63 22.46707601364767 27.11606590870514 27.967908359430112 22.448949718217083 64-65 22.310328632063893 26.976881514804575 27.9126089616054 22.80018089152613 66-67 22.21867859981512 27.25182323525528 27.724948735134436 22.804549429795166 68-69 22.25622309389037 27.13665111243585 27.85755264736497 22.749573146308812 70-71 22.223177315699402 27.023518657802985 27.78580783000167 22.967496196495947 72-73 22.336584177811662 27.09855122836326 27.630060251808086 22.934804342016992 74-75 22.17438454145278 27.227831314869732 27.520587954995207 23.07719618868228 76-77 22.21637524070738 27.047481819715046 27.681013351173554 23.05512958840402 78-79 22.196780015487523 27.09687427382764 27.658032237224507 23.04831347346033 80-81 22.065942786382646 27.193223962297765 27.630647891956134 23.11018535936346 82-83 22.245829619372763 27.09640227004744 27.551919144353317 23.10584896622647 84-85 22.186655404879318 26.965720688987183 27.516103172423072 23.331520733710427 86-87 22.084378308742377 26.91858732211515 27.700058489523645 23.296975879618834 88-89 22.123119687603403 26.824049233863985 27.5917489868565 23.461082091676115 90-91 21.97935553204246 26.91496784916396 27.689565451558252 23.41611116723533 92-93 22.02056272809324 26.796607012829593 27.617913965675218 23.56491629340195 94-95 21.849493764437344 26.760550220704317 27.60839240115645 23.781563613701888 96-97 21.865252796994696 26.527933196651237 27.687997316604257 23.918816689749807 98-99 21.709115394222223 26.581308274994754 27.533453716674757 24.176122614108273 100-101 22.172460359381695 25.706044010803375 28.130201118209268 23.991294511605656 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 221.0 1 160.5 2 116.5 3 146.0 4 149.5 5 166.5 6 285.0 7 607.0 8 991.5 9 1212.0 10 1245.0 11 1315.0 12 1430.0 13 1528.5 14 1661.0 15 1729.0 16 1851.0 17 2036.0 18 2163.0 19 2265.0 20 2560.5 21 2953.0 22 3508.5 23 4417.5 24 5403.0 25 6692.0 26 8595.0 27 10783.5 28 12765.0 29 14869.5 30 17759.0 31 21146.5 32 24248.5 33 26894.5 34 29817.5 35 32988.0 36 36461.5 37 40018.0 38 43071.0 39 44896.5 40 46459.0 41 49604.5 42 52620.5 43 54755.0 44 57251.0 45 59624.0 46 60651.0 47 61657.5 48 65656.5 49 66629.0 50 63943.5 51 62151.0 52 59876.0 53 58353.5 54 56037.5 55 51117.5 56 45584.5 57 40548.5 58 36092.5 59 31521.5 60 26182.5 61 21321.0 62 17136.5 63 13049.0 64 9512.0 65 6946.5 66 5047.0 67 3846.5 68 2909.0 69 2129.5 70 1602.0 71 1178.0 72 909.5 73 743.0 74 621.5 75 473.0 76 297.5 77 162.5 78 85.5 79 55.0 80 32.0 81 15.0 82 10.0 83 6.0 84 3.5 85 2.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.43134887933381044 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.474637620224875E-4 16-17 0.002460401549990688 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.228864683520729E-5 26-27 0.00367503016327723 28-29 9.343297025281094E-5 30-31 0.0 32-33 9.343297025281094E-5 34-35 0.01239544072020625 36-37 0.02606779870053425 38-39 0.01990122266384873 40-41 0.008876132174017039 42-43 0.009094142437940263 44-45 0.0037996074569476446 46-47 0.005699411185421467 48-49 0.003301298282265986 50-51 7.786080854400911E-4 52-53 0.0 54-55 0.0 56-57 3.1144323417603644E-5 58-59 0.001993236698726633 60-61 0.0028341334310019316 62-63 0.001650649141132993 64-65 0.0029275664012547425 66-67 0.002553834520243499 68-69 0.004858514453146169 70-71 9.343297025281094E-5 72-73 0.0 74-75 0.0 76-77 0.09732601068001139 78-79 0.016568780058165138 80-81 4.36020527846451E-4 82-83 0.0 84-85 0.0 86-87 0.10732333849706216 88-89 0.41683562462120716 90-91 0.4230956336281455 92-93 0.0 94-95 3.1144323417603644E-5 96-97 3.1144323417603644E-5 98-99 1.2457729367041458E-4 100-101 3.114432341760364E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1605429.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.67204605143768 #Duplication Level Percentage of deduplicated Percentage of total 1 80.15424209124862 51.83738835743314 2 12.102594240046765 15.653990640683373 3 3.584191229789465 6.953909408103098 4 1.5068884055914604 3.8981422544315367 5 0.7462083285863201 2.4129409695150414 6 0.4453121485479666 1.727954866689525 7 0.283381641845645 1.2828809414101499 8 0.19779934288118675 1.0233670569404976 9 0.1445051134903541 0.8410897218884766 >10 0.7654442190489201 8.913930636107875 >50 0.04563666386651878 2.004839367195573 >100 0.022736945963559844 2.426810834771624 >500 6.743094229551771E-4 0.24607918816341215 >1k 3.8531967026010123E-4 0.7766757566666479 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4864 0.3029719782064482 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3933 0.24498124800287024 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2552 0.158960626723449 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 6.228864683520729E-5 0.0 6 0.0 0.0 0.0 6.228864683520729E-5 0.0 7 6.228864683520729E-5 0.0 0.0 6.228864683520729E-5 0.0 8 6.228864683520729E-5 0.0 0.0 6.228864683520729E-5 0.0 9 6.228864683520729E-5 0.0 0.0 6.228864683520729E-5 0.0 10-11 6.228864683520729E-5 0.0 0.0 6.228864683520729E-5 0.0 12-13 6.228864683520729E-5 0.0 0.0 6.228864683520729E-5 0.0 14-15 6.228864683520729E-5 0.0 0.0 6.228864683520729E-5 0.0 16-17 6.228864683520729E-5 0.0 0.0 6.228864683520729E-5 0.0 18-19 6.228864683520729E-5 0.0 0.0 6.228864683520729E-5 0.0 20-21 6.228864683520729E-5 0.0 0.0 6.228864683520729E-5 0.0 22-23 1.2457729367041458E-4 0.0 0.0 6.228864683520729E-5 0.0 24-25 1.2457729367041458E-4 0.0 0.0 4.983091746816583E-4 0.0 26-27 1.2457729367041458E-4 0.0 0.0 8.720410556929019E-4 0.0 28-29 1.2457729367041458E-4 0.0 0.0 0.0029898550480899496 0.0 30-31 1.2457729367041458E-4 0.0 0.0 0.008938420820852246 0.0 32-33 1.8686594050562185E-4 0.0 0.0 0.02152072748156412 0.0 34-35 2.1801026392322549E-4 0.0 0.0 0.03908612588909257 0.0 36-37 3.114432341760364E-4 0.0 0.0 0.07094676874530109 0.0 38-39 3.114432341760364E-4 0.0 0.0 0.11781897548879458 0.0 40-41 3.114432341760364E-4 0.0 0.0 0.1699234285664455 0.0 42-43 4.0487620442884736E-4 0.0 0.0 0.22433256157699905 0.0 44-45 4.36020527846451E-4 0.0 0.0 0.287275239203976 0.0 46-47 4.983091746816583E-4 0.0 0.0 0.36600808880367797 0.0 48-49 5.605978215168656E-4 0.0 0.0 0.4540530911052435 0.0 50-51 5.605978215168656E-4 0.0 0.0 0.5458042678935038 0.0 52-53 5.605978215168656E-4 0.0 0.0 0.6456218244469235 0.0 54-55 5.605978215168656E-4 0.0 0.0 0.7544400904680306 0.0 56-57 5.605978215168656E-4 0.0 0.0 0.883035001859316 0.0 58-59 5.605978215168656E-4 0.0 0.0 1.0293198889518003 0.0 60-61 5.605978215168656E-4 0.0 0.0 1.1901491750803057 0.0 62-63 5.605978215168656E-4 0.0 0.0 1.3584530988290358 0.0 64-65 5.605978215168656E-4 0.0 0.0 1.5361314639264645 0.0 66-67 5.605978215168656E-4 0.0 0.0 1.7375418034681074 0.0 68-69 5.605978215168656E-4 0.0 0.0 1.9655493952083836 0.0 70-71 5.605978215168656E-4 0.0 0.0 2.221898321258679 0.0 72-73 5.605978215168656E-4 0.0 0.0 2.4858776065462878 6.228864683520729E-5 74-75 5.605978215168656E-4 0.0 0.0 2.75773640565855 6.228864683520729E-5 76-77 5.605978215168656E-4 0.0 0.0 3.049558716081496 6.228864683520729E-5 78-79 6.540307917696765E-4 0.0 0.0 3.385232233876428 6.228864683520729E-5 80-81 8.097524088576947E-4 0.0 0.0 3.7570331668357806 6.228864683520729E-5 82-83 8.72041055692902E-4 0.0 0.0 4.141821282660273 6.228864683520729E-5 84-85 9.031853791105057E-4 0.0 0.0 4.531000748086649 6.228864683520729E-5 86-87 9.343297025281093E-4 0.0 0.0 4.940953477232565 6.228864683520729E-5 88-89 9.343297025281093E-4 0.0 0.0 5.391985568966302 6.228864683520729E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4680 0.0 35.337795 1 GTATCAA 6820 0.0 30.748514 1 ATCAACG 8370 0.0 24.946661 3 TCAACGC 8485 0.0 24.66448 4 CAACGCA 8545 0.0 24.491293 5 TATCAAC 8580 0.0 24.446697 2 AACGCAG 8770 0.0 23.917067 6 GTACATG 6975 0.0 23.23221 1 TACATGG 6895 0.0 22.50602 2 ACATGGG 7115 0.0 21.009748 3 ACGCAGA 10175 0.0 20.521235 7 CGCAGAG 10270 0.0 20.2852 8 GAGTACT 5490 0.0 20.054617 12-13 GTACTTT 5635 0.0 19.243809 14-15 GGACCGT 175 1.0011887E-4 18.982141 6 GCAGAGT 11025 0.0 18.766924 9 AGTACTT 5695 0.0 18.62441 12-13 TCCGATA 130 0.00760553 18.25206 2 AGAGTAC 9815 0.0 17.889992 10-11 CATGGGG 3290 0.0 17.741667 4 >>END_MODULE