FastQCFastQC Report
Fri 27 May 2016
SRR939159_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939159_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2277236
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT209370.9194040494704984No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT119680.5255493940900284No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT93900.4123419794874137No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC82780.36351085263011823No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78070.34282788432995087No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT60960.26769294003783534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56340.24740518769244818No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT52050.22856656051458873No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC48520.2130653125104293No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC46550.20441447438912788No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG44670.19615885222260668No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC43110.18930844233974872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40480.17775935388339198No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA38930.17095285688439846No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA32980.14482469098503625No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30730.13494429211552952No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA26330.11562262321516083No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT26280.11540305879583845No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA26110.11465653977014241No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC25050.11000177408050814No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG94600.024.1379621
TACATGG96500.022.9280132
ACATGGG94750.022.2514133
ACCGTCG1150.003788956220.5909868
GAGTACT67350.019.96991212-13
CATGGGG47050.019.2331564
AGTACTT70550.019.1648112-13
GTACTTT70350.018.68157214-15
CATGGGA49400.018.1264064
GTCCGAT1600.001196052817.8023741
ATGGGAT23250.016.7096655
GTGTACG2606.6644934E-616.4329621
ATTCGCG1903.8708095E-916.2653180-81
TACTTTT81600.016.22206514-15
ACTTTTT83350.016.16859816-17
CGCGGAA2251.3278623E-1015.84947682-83
ATGGGGG24250.015.8252345
AGTACAT74250.015.7607662
GTCTAGG5900.015.2879151
AGAGTAC155300.015.14027110-11
Produced by FastQC (version 0.11.5)