##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939159_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2277236 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09172171878541 33.0 31.0 34.0 30.0 34.0 2 32.18176772192254 34.0 31.0 34.0 30.0 34.0 3 32.252020870915445 34.0 31.0 34.0 30.0 34.0 4 35.67698429148319 37.0 35.0 37.0 35.0 37.0 5 35.73018870244454 37.0 35.0 37.0 35.0 37.0 6 35.82327479453161 37.0 36.0 37.0 35.0 37.0 7 35.751048200537845 37.0 36.0 37.0 35.0 37.0 8 35.74463735862247 37.0 36.0 37.0 35.0 37.0 9 37.42144512031252 39.0 38.0 39.0 35.0 39.0 10-11 37.33215068618273 39.0 37.0 39.0 34.0 39.0 12-13 37.2460546469492 39.0 37.0 39.0 34.0 39.0 14-15 38.53665869501448 40.0 38.0 41.0 34.0 41.0 16-17 38.49134520971915 40.0 38.0 41.0 34.0 41.0 18-19 38.43683570784934 40.0 38.0 41.0 34.0 41.0 20-21 38.330670602432065 40.0 38.0 41.0 34.0 41.0 22-23 38.25087694029078 40.0 38.0 41.0 34.0 41.0 24-25 38.285957845388005 40.0 38.0 41.0 34.0 41.0 26-27 38.19858525862054 40.0 38.0 41.0 34.0 41.0 28-29 38.09403417124971 40.0 38.0 41.0 33.0 41.0 30-31 37.95681958303838 40.0 38.0 41.0 33.0 41.0 32-33 37.76288996836516 40.0 38.0 41.0 33.0 41.0 34-35 37.449909012504634 40.0 37.0 41.0 32.5 41.0 36-37 37.19297516814243 40.0 37.0 41.0 31.0 41.0 38-39 36.96474805422012 40.0 36.5 41.0 30.5 41.0 40-41 36.851003585047835 40.0 36.5 41.0 30.5 41.0 42-43 36.672850771725024 40.0 36.0 41.0 30.0 41.0 44-45 36.4212903273969 40.0 36.0 41.0 30.0 41.0 46-47 36.07076363626783 39.0 35.5 41.0 29.0 41.0 48-49 36.04719427411125 39.5 36.0 41.0 28.0 41.0 50-51 35.04366455650622 38.5 34.5 40.0 26.5 40.5 52-53 35.2284837847285 38.5 35.0 40.0 27.5 40.5 54-55 35.76270509512409 39.0 35.0 41.0 28.0 41.0 56-57 35.64455111371856 39.0 35.0 41.0 28.0 41.0 58-59 35.460625951811764 39.0 35.0 41.0 27.0 41.0 60-61 35.149048232155124 39.0 35.0 41.0 26.5 41.0 62-63 34.809689465650465 38.0 35.0 40.0 26.0 41.0 64-65 34.44824493377059 37.5 34.0 40.0 26.0 41.0 66-67 34.03606938411302 37.0 34.0 40.0 25.0 41.0 68-69 33.62713526397791 36.5 34.0 39.0 23.5 41.0 70-71 33.17675989664664 36.0 33.0 39.0 22.5 41.0 72-73 32.719785081563785 35.0 33.0 38.5 21.5 40.0 74-75 32.2828738435542 35.0 32.5 37.0 20.5 39.5 76-77 31.850460602238854 35.0 32.0 37.0 19.5 39.0 78-79 31.42113685186779 35.0 32.0 36.0 18.0 39.0 80-81 30.982378418398447 35.0 31.0 36.0 15.0 37.5 82-83 30.555227038392154 35.0 31.0 35.5 9.5 37.0 84-85 30.176200007377364 34.0 31.0 35.0 7.0 37.0 86-87 29.83148079513937 34.0 30.5 35.0 2.0 36.0 88-89 29.43476455668187 34.0 30.0 35.0 2.0 36.0 90-91 29.041577157571723 34.0 29.5 35.0 2.0 36.0 92-93 28.633158135564344 34.0 29.0 35.0 2.0 35.0 94-95 28.172438429745533 34.0 29.0 35.0 2.0 35.0 96-97 27.614768956752837 34.0 28.0 35.0 2.0 35.0 98-99 26.947493364763247 34.0 26.5 35.0 2.0 35.0 100-101 24.37497321314084 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1572360472460801 1101 2 0.2001629462337533 1101 3 0.14343015123183278 1101 4 0.11729640396182361 1101 5 0.1548566013745898 1101 6 0.21757107016151878 1101 7 0.20682733278698606 1101 8 0.2543171066531684 1101 9 0.23747800009044795 1101 10-11 0.26444165155972854 1101 12-13 0.2164623256839917 1101 14-15 0.3239151966184082 1101 16-17 0.2856298997490754 1101 18-19 0.29408883837435695 1101 20-21 0.33684247604762874 1101 22-23 0.3160312691156548 1101 24-25 0.37631513565900576 1101 26-27 0.41980882625998106 1101 28-29 0.39417025349905543 1101 30-31 0.3532224952461718 1101 32-33 0.3855167214121735 1101 34-35 0.3826660481649853 1101 36-37 0.14820900022907324 1101 38-39 0.22133462437746942 1101 40-41 -6.09741063634317E-4 1101 42-43 -0.12120440698976154 1101 44-45 -0.046265840388919344 1101 46-47 -0.12473996830296841 1101 48-49 -0.020173810051701935 1101 50-51 -0.29294393114134465 1101 52-53 -0.18939544591145818 1101 54-55 -0.08535714942773609 1101 56-57 -0.1636139069107685 1101 58-59 -0.08355904475497766 1101 60-61 -0.3051659530442734 1101 62-63 -0.30676387916476955 1101 64-65 -0.3086431078362679 1101 66-67 -0.321951716928659 1101 68-69 -0.3792941989040841 1101 70-71 -0.36434467651739055 1101 72-73 -0.31115866676832127 1101 74-75 -0.4308357253206907 1101 76-77 -0.49495688028048335 1101 78-79 -0.5859134052567292 1101 80-81 -0.6685166242410645 1101 82-83 -0.7138864901750352 1101 84-85 -0.7639111898735571 1101 86-87 -0.9149868073696155 1101 88-89 -0.8819742115724409 1101 90-91 -0.8595899473957935 1101 92-93 -0.9399225634594615 1101 94-95 -1.1309051012119582 1101 96-97 -1.0922402178432726 1101 98-99 -1.3031176547353382 1101 100-101 -1.2686366113280805 1102 1 0.29254232382605494 1102 2 0.3387127007348667 1102 3 0.4409830278650517 1102 4 0.4152479882750413 1102 5 0.3823569784780574 1102 6 0.39444307516438215 1102 7 0.3666653865760452 1102 8 0.46525908394830395 1102 9 0.37701016410548505 1102 10-11 0.38910649519309715 1102 12-13 0.4625327523115601 1102 14-15 0.5903442945687942 1102 16-17 0.5846362094727553 1102 18-19 0.49656713907756966 1102 20-21 0.5893280600871051 1102 22-23 0.6268911762770486 1102 24-25 0.519135926518068 1102 26-27 0.5949414981163201 1102 28-29 0.5905533202467552 1102 30-31 0.6122192266711366 1102 32-33 0.4972036429981941 1102 34-35 0.44620106134873794 1102 36-37 0.5164773799157274 1102 38-39 0.5040447602718459 1102 40-41 0.43283298461694386 1102 42-43 0.3378244332107343 1102 44-45 0.31160379798244264 1102 46-47 0.2939734710954056 1102 48-49 0.3359509324118619 1102 50-51 0.3614604691839318 1102 52-53 0.31423186253744007 1102 54-55 0.32866183692331674 1102 56-57 0.27473659724357447 1102 58-59 0.26269904097726027 1102 60-61 0.34882253385102047 1102 62-63 0.30855500806563896 1102 64-65 0.36093495284026034 1102 66-67 0.3527953132683379 1102 68-69 0.19716453174623894 1102 70-71 0.17065363007458956 1102 72-73 0.04827475435315165 1102 74-75 -0.029786381558345454 1102 76-77 -0.012027830325077815 1102 78-79 0.08123282317819402 1102 80-81 0.10784588357590863 1102 82-83 -0.09955816255064676 1102 84-85 -0.049881362606349455 1102 86-87 -0.0527674777832523 1102 88-89 -0.10699514954713862 1102 90-91 -0.09266615179182125 1102 92-93 -0.14827539879888008 1102 94-95 -0.4124160092693465 1102 96-97 -0.4885101147407269 1102 98-99 -0.5862942863773064 1102 100-101 -0.6684648643079711 1103 1 0.2172857911667947 1103 2 0.23648078900320968 1103 3 0.3045393123409923 1103 4 0.24832999305992587 1103 5 0.34847418208894254 1103 6 0.2779081563050525 1103 7 0.18766648781969053 1103 8 0.25494538470079675 1103 9 0.2987329710228366 1103 10-11 0.2969376163137625 1103 12-13 0.359172903878445 1103 14-15 0.4330737896479633 1103 16-17 0.5057044778881661 1103 18-19 0.49777006463622797 1103 20-21 0.510852221025111 1103 22-23 0.4531645901199397 1103 24-25 0.29794946374494913 1103 26-27 0.49892770215305404 1103 28-29 0.473638242644455 1103 30-31 0.37199912692602766 1103 32-33 0.5254398032707357 1103 34-35 0.40361359814479414 1103 36-37 0.22868256134779585 1103 38-39 0.2864193991396675 1103 40-41 0.33511542691960017 1103 42-43 0.4039946504673608 1103 44-45 0.33060751390379295 1103 46-47 0.33464350398372744 1103 48-49 0.22547372269261245 1103 50-51 0.3937398200585349 1103 52-53 0.3378892329861216 1103 54-55 0.38589919279635154 1103 56-57 0.3023593139011638 1103 58-59 0.3078049627380608 1103 60-61 0.4083842959898405 1103 62-63 0.31184731686578004 1103 64-65 0.2829801870128321 1103 66-67 0.19886851679479634 1103 68-69 0.12721817857603668 1103 70-71 0.1699485610016538 1103 72-73 0.012714640975957536 1103 74-75 0.01184969155827531 1103 76-77 0.11732360296773692 1103 78-79 0.26237908497124707 1103 80-81 0.14194557654371565 1103 82-83 0.1621288632596709 1103 84-85 0.20113005581285037 1103 86-87 0.1921995159457417 1103 88-89 0.18691734441588892 1103 90-91 0.08971755029880413 1103 92-93 0.032822070575498685 1103 94-95 -0.14990013311021855 1103 96-97 -0.11083568805164745 1103 98-99 -1.189291559668959E-4 1103 100-101 -0.0840841493562614 1104 1 0.14230904919975984 1104 2 0.1460428223516459 1104 3 0.21825678091283152 1104 4 0.21735506402674076 1104 5 0.2592981128157916 1104 6 0.2092659483424555 1104 7 0.1691161192693258 1104 8 0.1663334718170404 1104 9 0.2551843915519285 1104 10-11 0.2634549942389057 1104 12-13 0.32077806596903713 1104 14-15 0.3410578549135863 1104 16-17 0.376854378892439 1104 18-19 0.4518443949241515 1104 20-21 0.3346595013857936 1104 22-23 0.3055386985620103 1104 24-25 0.34956440613658657 1104 26-27 0.3544124234144732 1104 28-29 0.3628166973568909 1104 30-31 0.32197407981360016 1104 32-33 0.3532265927080118 1104 34-35 0.39152955911900733 1104 36-37 0.43072270829085113 1104 38-39 0.3432673989488251 1104 40-41 0.20492232560999213 1104 42-43 0.21414350187834685 1104 44-45 0.27586764605712943 1104 46-47 0.2900626256649801 1104 48-49 0.40519927790359844 1104 50-51 0.3669066099728937 1104 52-53 0.37993975659004064 1104 54-55 0.39090801892168514 1104 56-57 0.3234293026895969 1104 58-59 0.3410150483753327 1104 60-61 0.23766496677536253 1104 62-63 0.24064314711792179 1104 64-65 0.2798308108052012 1104 66-67 0.2484218432898757 1104 68-69 0.32694862393754676 1104 70-71 0.32979267254479794 1104 72-73 0.37470028063150096 1104 74-75 0.26283308890089785 1104 76-77 0.19854900312517998 1104 78-79 0.1308101815576812 1104 80-81 0.10312434136325521 1104 82-83 0.047201380613746124 1104 84-85 0.1765659948410203 1104 86-87 0.037160871829424735 1104 88-89 0.2925243194403393 1104 90-91 0.16021801545946346 1104 92-93 0.09427575727190174 1104 94-95 -0.03851860595198886 1104 96-97 -0.1657902454333886 1104 98-99 -0.06968788027831607 1104 100-101 -0.128846321060184 1105 1 0.14768103229165064 1105 2 0.028073786604792872 1105 3 0.08395743510675402 1105 4 0.10715463900903188 1105 5 0.15548290755260297 1105 6 0.1642031910979469 1105 7 0.0927451130699879 1105 8 0.07595609498189759 1105 9 0.08251886382160478 1105 10-11 0.12795525119405937 1105 12-13 0.16131331975276453 1105 14-15 0.2714757889941737 1105 16-17 0.15303428916647732 1105 18-19 0.19184490420463618 1105 20-21 0.15382152665536353 1105 22-23 0.2600834481117573 1105 24-25 0.09808795572893558 1105 26-27 0.1276260063878425 1105 28-29 0.23893861910363512 1105 30-31 0.08340210227749623 1105 32-33 0.23730301255884 1105 34-35 0.05250490068416269 1105 36-37 0.11423463702862335 1105 38-39 0.28066120239434156 1105 40-41 0.3254013039007546 1105 42-43 0.27432772251716386 1105 44-45 0.21079109194145218 1105 46-47 0.45393068035244966 1105 48-49 0.5412877028842686 1105 50-51 0.39985069097627246 1105 52-53 0.40555147189299845 1105 54-55 0.531778587602787 1105 56-57 0.37843101572393323 1105 58-59 0.48649055429918775 1105 60-61 0.5934625463041456 1105 62-63 0.3590061807628473 1105 64-65 0.42076295612607595 1105 66-67 0.46357907206329685 1105 68-69 0.3595800761739838 1105 70-71 0.42514722435482355 1105 72-73 0.44946821077673604 1105 74-75 0.4602254571014228 1105 76-77 0.35949066273136054 1105 78-79 0.44909381374606383 1105 80-81 0.2966475668680246 1105 82-83 0.20469370668288533 1105 84-85 0.29922596678429514 1105 86-87 0.3323228230218369 1105 88-89 0.23064882462805514 1105 90-91 0.20436360485902227 1105 92-93 0.23887576375365427 1105 94-95 0.001814671823471059 1105 96-97 0.062148650168740716 1105 98-99 0.12539663713213045 1105 100-101 0.14095114735285463 1106 1 0.2402748374568091 1106 2 0.25688424629986883 1106 3 0.26224660985411674 1106 4 0.33852935898744363 1106 5 0.32774471475753586 1106 6 0.2901952132523036 1106 7 0.23344806564010412 1106 8 0.2582890890153777 1106 9 0.3760350619171646 1106 10-11 0.31781236825647596 1106 12-13 0.34604390045817723 1106 14-15 0.4229811096523761 1106 16-17 0.44522007468531655 1106 18-19 0.4217241915029817 1106 20-21 0.4408199267209696 1106 22-23 0.47311844890874966 1106 24-25 0.4214784144583561 1106 26-27 0.6268465426932579 1106 28-29 0.44111073341792206 1106 30-31 0.3780535989999194 1106 32-33 0.4427550254403272 1106 34-35 0.4465110662849767 1106 36-37 0.3851956625210846 1106 38-39 0.49982079700708226 1106 40-41 0.5146195054528064 1106 42-43 0.5603062388177804 1106 44-45 0.6180544236808032 1106 46-47 0.5183682197472805 1106 48-49 0.6487845761005531 1106 50-51 0.5105150242123813 1106 52-53 0.43158099560992724 1106 54-55 0.29477995604604246 1106 56-57 0.4412633295042099 1106 58-59 0.4943668745426919 1106 60-61 0.5437098118978838 1106 62-63 0.565908981607059 1106 64-65 0.5077434321644247 1106 66-67 0.4938333088469733 1106 68-69 0.6095653599523416 1106 70-71 0.5371089000066505 1106 72-73 0.5157543721710312 1106 74-75 0.40877622343626285 1106 76-77 0.5327376300322513 1106 78-79 0.6126409721263393 1106 80-81 0.6990905919411574 1106 82-83 0.7360710061338551 1106 84-85 0.7874536508743084 1106 86-87 0.812345800169691 1106 88-89 0.8414609814837988 1106 90-91 0.704670165251418 1106 92-93 0.9520658636056964 1106 94-95 0.8813265165630355 1106 96-97 0.8824067032489964 1106 98-99 0.9021105478307909 1106 100-101 0.8153168782001039 1107 1 0.05907474852952532 1107 2 0.07492057006594166 1107 3 0.03589697402652092 1107 4 0.08925631431411318 1107 5 0.19255186633056098 1107 6 0.15545142237291287 1107 7 0.10754963475201862 1107 8 0.05572813466387316 1107 9 0.13639559639037913 1107 10-11 0.12134842801965817 1107 12-13 0.15373516360463668 1107 14-15 0.30690794421983014 1107 16-17 0.24057836071286687 1107 18-19 0.17150945231004044 1107 20-21 0.1096121956047611 1107 22-23 0.07699695452608069 1107 24-25 0.2762484035192969 1107 26-27 0.16757183063324987 1107 28-29 -0.016891333131013653 1107 30-31 0.06666566380567929 1107 32-33 0.0876395589131107 1107 34-35 0.23652266220140206 1107 36-37 0.1960347496016226 1107 38-39 0.21705159616530523 1107 40-41 0.1734971394816398 1107 42-43 0.1971907899749752 1107 44-45 0.2197236419543529 1107 46-47 0.44086947660314735 1107 48-49 0.24416730450094803 1107 50-51 0.180042143246105 1107 52-53 0.21768844352303773 1107 54-55 0.2574424946675222 1107 56-57 0.37483786547807085 1107 58-59 0.21408833254601944 1107 60-61 0.008798271813653002 1107 62-63 0.0012175365638711355 1107 64-65 -0.13743005184002755 1107 66-67 -0.12412135086719189 1107 68-69 0.11986115687475518 1107 70-71 -0.004379343654115075 1107 72-73 -0.03627156579713642 1107 74-75 0.1214608675628952 1107 76-77 0.051134463008107645 1107 78-79 0.02724898467721104 1107 80-81 -0.12253440269036275 1107 82-83 -0.07624725503447749 1107 84-85 -0.07449683784683003 1107 86-87 -0.043959258327788575 1107 88-89 -0.07785297732164764 1107 90-91 -0.16073446654665347 1107 92-93 -0.07638139518534359 1107 94-95 -0.12576903479878965 1107 96-97 0.017101862017049996 1107 98-99 0.1419259100200172 1107 100-101 0.04497191863423211 1108 1 0.09626344111362783 1108 2 0.10239144365883845 1108 3 0.20078994195473143 1108 4 0.07580825698303073 1108 5 0.10950408202013762 1108 6 0.17037440955737537 1108 7 0.2468070527217563 1108 8 0.27977307080583813 1108 9 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#Quality Count 2 7478.0 3 1812.0 4 1926.0 5 2131.0 6 2400.0 7 2745.0 8 3413.0 9 4089.0 10 4947.0 11 6298.0 12 8080.0 13 12724.0 14 10723.0 15 10666.0 16 13306.0 17 17688.0 18 16911.0 19 11570.0 20 11795.0 21 12537.0 22 13384.0 23 14638.0 24 16522.0 25 18764.0 26 21533.0 27 25497.0 28 30263.0 29 35797.0 30 43045.0 31 52788.0 32 66461.0 33 87642.0 34 122344.0 35 183723.0 36 298491.0 37 488856.0 38 500422.0 39 93774.0 40 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11277664728127 18.827481728699052 14.405179152606454 22.654562471413225 2 14.721236891486214 20.93840515353866 40.283740079033585 24.056617875941537 3 20.552226535537958 24.556781162485 29.648423246182865 25.242569055794178 4 12.594418679905948 16.251664142972963 38.147740722567804 33.00617645455329 5 13.461171448246628 37.92919667521814 34.13613629107935 14.47349558545588 6 31.223845855311126 36.19384207105006 17.436785039466635 15.145527034172185 7 28.01669780912683 32.989733317685186 21.071203927510375 17.922364945677614 8 25.3917981305519 34.196024785308865 20.05810375507276 20.354073329066473 9 25.687664108904578 18.182277688748865 21.40278995027176 34.72726825207479 10-11 25.087316442082162 26.071053486910937 27.2292585953376 21.6123714756693 12-13 26.74702311489583 23.789038170851292 27.450988148720523 22.01295056553235 14-15 23.018793201815498 25.3051543727292 26.990525160016492 24.68552726543881 16-17 21.786126316575945 28.572637195289442 27.295231649244172 22.34600483889044 18-19 21.577447181392184 28.250584236033777 28.916216607666133 21.255751974907906 20-21 22.925844867010746 26.414621553167457 28.263746611443054 22.395786968378744 22-23 22.1313819578368 26.390808973929758 28.637720638196335 22.84008843003711 24-25 22.937940453404003 26.45991876771762 28.50077323248113 22.101367546397245 26-27 22.51876937864557 26.013328792641268 29.60930627884056 21.858595549872607 28-29 21.592264103159362 27.678065797156503 28.932405433735024 21.797264665949108 30-31 22.04419174183888 26.596619509142332 28.59788522034871 22.761303528670073 32-33 22.028169236984617 26.819089210203668 28.794008662926185 22.35873288988553 34-35 23.14077697386552 26.410092769217652 28.409793606059395 22.03933665085743 36-37 22.726110259002144 26.01910504065299 29.259936190020376 21.994848510324495 38-39 22.207956627887047 26.90560948906156 29.010792069182546 21.87564181386885 40-41 22.221702697999564 27.060232731658118 28.701806745958315 22.016257824384002 42-43 21.877479267914932 27.17840227260894 27.866772801763585 23.07734565771255 44-45 22.41797117962067 26.495544515242774 28.035696831549867 23.050787473586688 46-47 23.010396694911496 27.178849763709472 27.811716047124385 21.999037494254644 48-49 22.807959500732657 26.87794608336423 27.932974948360783 22.381119467542327 50-51 21.74424867667544 27.307761304884405 28.773640173549747 22.174349844890408 52-53 22.473246739032575 27.815425448344406 27.33993941458816 22.37138839803486 54-55 21.777490070914006 26.8670112530009 28.01468364048909 23.340815035596002 56-57 22.655940318327257 26.880002470560076 27.58925174014561 22.874805470967058 58-59 23.440287799612992 26.545354860080995 27.49035896879451 22.523998371511507 60-61 22.565708676232536 26.862303378799467 28.739161901478266 21.832826043489735 62-63 22.06247846327141 27.05081498731291 28.442909414037732 22.443797135377945 64-65 21.799905771028012 27.735271757899955 27.934719403996233 22.530103067075803 66-67 22.195268254508427 27.000151395375422 27.341541346165304 23.463039003950843 68-69 21.782767825493124 27.108707906956624 27.90961409679651 23.19891017075375 70-71 22.695403928769668 26.946822234262907 27.472696032938014 22.88507780402941 72-73 22.342202057370958 26.289796101221786 28.4981827579046 22.869819083502655 74-75 21.719979209818614 27.9966276648455 27.553964535305237 22.729428590030647 76-77 22.132316631060906 26.703130026712046 27.529711798533025 23.63484154369403 78-79 22.1321856538744 26.87359929123778 27.94073558346807 23.05347947141975 80-81 22.666882864041636 27.013222234355748 27.444911033677155 22.874983867925465 82-83 22.338543290434423 26.68922042158444 28.112807607115442 22.859428680865694 84-85 22.001405901255072 27.03473979353638 27.853083760119535 23.11077054508901 86-87 22.01525452973658 27.15739199084234 27.807589402144057 23.019764077277024 88-89 21.891075999646926 27.01019507458734 27.227381057463145 23.871347868302585 90-91 22.15948316197801 26.3053814929189 27.650820934129282 23.884314410973804 92-93 22.64254001210261 26.70194608379932 27.754480327079435 22.901033577018637 94-95 22.19728071619666 26.38050904802114 27.891572939228602 23.5306372965536 96-97 21.403788555764017 26.343993589147985 28.606264264024343 23.645953591063652 98-99 21.843581940935238 26.815366169454407 27.419802261189925 23.921249628420433 100-101 21.886194928536707 25.665509023022757 28.082151729945032 24.366144318495504 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2674.0 1 1538.5 2 495.0 3 580.0 4 504.0 5 522.0 6 966.5 7 1859.5 8 2731.5 9 2987.0 10 2680.5 11 2517.5 12 2664.5 13 2883.5 14 3059.5 15 3195.5 16 3467.0 17 3587.5 18 3573.0 19 3717.0 20 3979.5 21 4350.5 22 5000.5 23 6037.5 24 7210.5 25 8842.0 26 11202.5 27 14100.5 28 16707.5 29 19818.5 30 23889.0 31 28308.5 32 33409.0 33 37989.5 34 42015.5 35 45639.0 36 49861.5 37 54560.0 38 58193.0 39 59937.5 40 61281.5 41 64966.5 42 68261.5 43 70853.5 44 73989.0 45 77504.0 46 81591.0 47 97808.5 48 136125.5 49 135268.5 50 99770.5 51 84512.5 52 78044.0 53 75526.0 54 72920.0 55 66999.0 56 58992.0 57 51896.0 58 46116.0 59 40346.5 60 34284.5 61 28419.0 62 22937.5 63 17764.5 64 13283.0 65 9981.5 66 7618.0 67 6100.5 68 4615.0 69 3187.0 70 2408.5 71 1901.0 72 1544.0 73 1275.0 74 966.0 75 743.0 76 526.5 77 322.5 78 195.0 79 114.0 80 78.5 81 59.5 82 44.0 83 37.0 84 28.0 85 16.5 86 14.0 87 14.5 88 13.5 89 9.5 90 7.0 91 8.0 92 5.0 93 3.5 94 5.5 95 4.5 96 2.5 97 2.5 98 4.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3438378806588338 2 0.12119955946594907 3 0.11531523302810952 4 0.12260477174961225 5 0.13885253877946774 6 0.08883576405783151 7 0.09691573468889478 8 0.10275614824286987 9 0.0908996695994618 10-11 0.08934076222227297 12-13 0.09322705244427895 14-15 0.09476400337953554 16-17 0.08435664990365514 18-19 0.09908942244018626 20-21 0.1091015599612864 22-23 0.11722544347621414 24-25 0.13248517061911896 26-27 0.1311458276612525 28-29 0.1252395447814807 30-31 0.11529327658617729 32-33 0.10912351640321863 34-35 0.3324864001798672 36-37 0.4957545023879826 38-39 0.470328942630452 40-41 0.448109023395028 42-43 0.48049477524507783 44-45 0.48727931580213907 46-47 0.49513972201388 48-49 0.5057007705832861 50-51 0.4943492901043195 52-53 0.4981477545585965 54-55 0.4848201943057285 56-57 0.4629954910250848 58-59 0.4436518656827839 60-61 0.4012539763116339 62-63 0.472458717497879 64-65 0.5040540374383683 66-67 0.5113655326018033 68-69 0.523748965851585 70-71 0.5167887737590658 72-73 0.5084014129409512 74-75 0.5156909516624539 76-77 0.49257081830780824 78-79 0.4720415451011665 80-81 0.47375414757188106 82-83 0.4871914900344101 84-85 0.4862912759151884 86-87 0.48984821950821084 88-89 0.5021877398741281 90-91 0.5167668173171336 92-93 0.5097846687826822 94-95 0.5143516087045875 96-97 0.5138905234240104 98-99 0.5080940227538999 100-101 0.49544711220093135 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2277236.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.79988874569509 #Duplication Level Percentage of deduplicated Percentage of total 1 72.04306616511089 35.156936137561615 2 14.614667673067263 14.26388313001978 3 5.503652117254188 8.057328330510408 4 2.7081639016780223 5.286323884279801 5 1.4273442121218833 3.4827119376679856 6 0.8956843970061017 2.6225579355091653 7 0.5679273937777674 1.940035554239133 8 0.41042085865746464 1.6022793793117538 9 0.29959563224279373 1.3158210169930062 >10 1.3840010224591743 12.074290019984094 >50 0.09233045817858919 3.0722707468033295 >100 0.04728483670677887 4.061091268460373 >500 0.0027052300334871678 0.8561167864569695 >1k 0.0024347070301383236 2.8941191884708117 >5k 5.410460066974052E-4 1.866250985023828 >10k+ 1.8034866889913507E-4 1.4479836987080656 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 20937 0.9194040494704984 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11968 0.5255493940900284 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9390 0.4123419794874137 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8278 0.36351085263011823 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7807 0.34282788432995087 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6096 0.26769294003783534 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5634 0.24740518769244818 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5205 0.22856656051458873 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4852 0.2130653125104293 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4655 0.20441447438912788 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4467 0.19615885222260668 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4311 0.18930844233974872 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4048 0.17775935388339198 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3893 0.17095285688439846 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3298 0.14482469098503625 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3073 0.13494429211552952 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2633 0.11562262321516083 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2628 0.11540305879583845 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2611 0.11465653977014241 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2505 0.11000177408050814 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.39128838644743E-5 0.0 10-11 0.0 0.0 0.0 4.39128838644743E-5 0.0 12-13 0.0 0.0 0.0 4.39128838644743E-5 0.0 14-15 0.0 0.0 0.0 8.78257677289486E-5 0.0 16-17 0.0 0.0 0.0 1.0978220966118577E-4 0.0 18-19 0.0 0.0 0.0 1.756515354578972E-4 0.0 20-21 0.0 0.0 0.0 2.4152086125460865E-4 0.0 22-23 0.0 0.0 0.0 3.7325951284803154E-4 0.0 24-25 0.0 0.0 0.0 5.708674902381659E-4 0.0 26-27 0.0 0.0 0.0 0.0012076043062730433 0.0 28-29 0.0 0.0 0.0 0.003996072431667161 0.0 30-31 0.0 0.0 0.0 0.010934308082254101 0.0 32-33 0.0 0.0 0.0 0.02380078305454507 4.39128838644743E-5 34-35 0.0 0.0 0.0 0.04424223049345786 4.39128838644743E-5 36-37 0.0 0.0 0.0 0.07443233815028394 4.39128838644743E-5 38-39 4.39128838644743E-5 0.0 0.0 0.12425150489453005 4.39128838644743E-5 40-41 4.39128838644743E-5 0.0 0.0 0.18342411590190916 4.39128838644743E-5 42-43 4.39128838644743E-5 0.0 0.0 0.24542910791854688 4.39128838644743E-5 44-45 8.78257677289486E-5 0.0 0.0 0.31834645157550645 4.39128838644743E-5 46-47 1.317386515934229E-4 0.0 0.0 0.40505244076591096 4.39128838644743E-5 48-49 1.317386515934229E-4 0.0 0.0 0.5006727453808038 4.39128838644743E-5 50-51 1.317386515934229E-4 0.0 0.0 0.6092693071776487 4.39128838644743E-5 52-53 1.5369509352566006E-4 0.0 0.0 0.7134965370299784 4.39128838644743E-5 54-55 1.756515354578972E-4 0.0 0.0 0.8305243725288025 4.39128838644743E-5 56-57 1.756515354578972E-4 0.0 0.0 0.9826605586772736 4.39128838644743E-5 58-59 1.756515354578972E-4 0.0 0.0 1.140461506844262 4.39128838644743E-5 60-61 1.756515354578972E-4 0.0 0.0 1.3260153976135982 4.39128838644743E-5 62-63 1.756515354578972E-4 0.0 0.0 1.5116132012667989 4.39128838644743E-5 64-65 1.756515354578972E-4 0.0 0.0 1.7011192515839377 4.39128838644743E-5 66-67 1.9760797739013436E-4 0.0 0.0 1.925558001015266 4.39128838644743E-5 68-69 2.195644193223715E-4 0.0 0.0 2.1855881428187507 4.39128838644743E-5 70-71 2.195644193223715E-4 0.0 0.0 2.4722953615699033 4.39128838644743E-5 72-73 2.195644193223715E-4 0.0 0.0 2.755204115866779 4.39128838644743E-5 74-75 2.4152086125460865E-4 0.0 0.0 3.0599814863281627 4.39128838644743E-5 76-77 2.634773031868458E-4 0.0 0.0 3.3742220832623406 4.39128838644743E-5 78-79 2.634773031868458E-4 0.0 0.0 3.7633341471854473 4.39128838644743E-5 80-81 3.073901870513201E-4 0.0 0.0 4.193153454450922 4.39128838644743E-5 82-83 3.073901870513201E-4 0.0 0.0 4.61102845730526 4.39128838644743E-5 84-85 3.073901870513201E-4 0.0 0.0 5.051540551791733 4.39128838644743E-5 86-87 3.073901870513201E-4 0.0 0.0 5.514184739745902 4.39128838644743E-5 88-89 3.073901870513201E-4 0.0 0.0 6.014132922542942 4.39128838644743E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9460 0.0 24.137962 1 TACATGG 9650 0.0 22.928013 2 ACATGGG 9475 0.0 22.251413 3 ACCGTCG 115 0.0037889562 20.590986 8 GAGTACT 6735 0.0 19.969912 12-13 CATGGGG 4705 0.0 19.233156 4 AGTACTT 7055 0.0 19.16481 12-13 GTACTTT 7035 0.0 18.681572 14-15 CATGGGA 4940 0.0 18.126406 4 GTCCGAT 160 0.0011960528 17.802374 1 ATGGGAT 2325 0.0 16.709665 5 GTGTACG 260 6.6644934E-6 16.432962 1 ATTCGCG 190 3.8708095E-9 16.26531 80-81 TACTTTT 8160 0.0 16.222065 14-15 ACTTTTT 8335 0.0 16.168598 16-17 CGCGGAA 225 1.3278623E-10 15.849476 82-83 ATGGGGG 2425 0.0 15.825234 5 AGTACAT 7425 0.0 15.760766 2 GTCTAGG 590 0.0 15.287915 1 AGAGTAC 15530 0.0 15.140271 10-11 >>END_MODULE