FastQCFastQC Report
Fri 27 May 2016
SRR939159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939159_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2277236
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT233511.0254097511193394No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT111740.4906825643016358No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT86340.3791438392858711No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77740.3413787591624232No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC74990.3293027160996928No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT63980.2809546309649066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59440.2610181816904352No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC55610.24419954717034162No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC52890.2322552427592046No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT51430.22584396171499133No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG48860.21455835056182143No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA44000.1932166890036869No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42460.1864541048885579No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC39790.17472936489674326No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA36450.16006246168600882No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30290.13301212522549266No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT26530.11650088089245032No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC25930.11386610786058186No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25880.1136465434412595No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA25260.11092394464166208No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95100.024.2297231
TGCACCG3650.023.399765
TACATGG97300.022.8713472
ACATGGG94750.022.5353873
CATGGGG45850.021.629114
GAGTACT76500.021.1826412-13
GTAAGCG3700.020.51870794-95
GTACTTT79450.020.36625914-15
AGTACTT80200.020.29413612-13
GCACCGT4550.019.8140816
ATCGATC1300.007611887518.2498118
CGTACAC2352.55546E-618.1721533
TAGTACT5650.017.63614
TACTTTT91950.017.597614-15
ATGGGGA27900.017.3471325
ACTTTTT96400.016.7847116-17
AGTACAT75500.016.5287692
TACACTG18100.016.2534245
CATGGGA53900.015.933884
AGAGTAC161750.015.90031610-11
Produced by FastQC (version 0.11.5)