##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939159_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2277236 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50319729707417 34.0 31.0 34.0 31.0 34.0 2 32.70690082187353 34.0 31.0 34.0 31.0 34.0 3 32.80661424639344 34.0 31.0 34.0 31.0 34.0 4 36.25077023198298 37.0 35.0 37.0 35.0 37.0 5 36.213986604813904 37.0 37.0 37.0 35.0 37.0 6 36.25984351204706 37.0 37.0 37.0 35.0 37.0 7 36.216404448199484 37.0 37.0 37.0 35.0 37.0 8 36.203443121398045 37.0 37.0 37.0 35.0 37.0 9 37.947957523945696 39.0 38.0 39.0 35.0 39.0 10-11 37.885533163888155 39.0 38.0 39.0 35.0 39.0 12-13 37.82877620062216 39.0 38.0 39.0 35.0 39.0 14-15 39.16576916050862 40.5 39.0 41.0 36.0 41.0 16-17 39.12947164896392 40.0 38.5 41.0 36.0 41.0 18-19 39.169014322626204 40.5 38.5 41.0 36.0 41.0 20-21 39.23926461728165 41.0 39.0 41.0 36.0 41.0 22-23 39.204399324444196 41.0 39.0 41.0 36.0 41.0 24-25 39.14159270273261 41.0 39.0 41.0 35.5 41.0 26-27 38.99850608369093 40.0 38.5 41.0 35.0 41.0 28-29 38.86912906699174 40.0 38.0 41.0 35.0 41.0 30-31 38.71211240293057 40.0 38.0 41.0 35.0 41.0 32-33 38.58428375451644 40.0 38.0 41.0 34.5 41.0 34-35 38.52845071832695 40.0 38.0 41.0 34.0 41.0 36-37 38.4286514441191 40.0 38.0 41.0 34.0 41.0 38-39 38.30511023012107 40.0 38.0 41.0 34.0 41.0 40-41 38.18001801306496 40.0 38.0 41.0 34.0 41.0 42-43 38.0325919228398 40.0 38.0 41.0 33.0 41.0 44-45 37.827552568113276 40.0 38.0 41.0 33.0 41.0 46-47 37.55695742558083 40.0 37.0 41.0 33.0 41.0 48-49 37.2577179089036 40.0 37.0 41.0 32.0 41.0 50-51 37.01220251216826 40.0 37.0 41.0 31.0 41.0 52-53 36.81207547219524 40.0 36.0 41.0 31.0 41.0 54-55 36.59496753959625 39.0 36.0 41.0 31.0 41.0 56-57 36.32037610506772 39.0 35.0 41.0 30.5 41.0 58-59 36.0410837963215 39.0 35.0 40.0 30.0 41.0 60-61 35.650475840009555 38.0 35.0 40.0 29.0 41.0 62-63 35.29695472932977 38.0 35.0 40.0 28.0 41.0 64-65 34.90364810673992 37.0 34.0 40.0 28.0 41.0 66-67 34.5259024536763 37.0 34.0 39.0 28.0 41.0 68-69 34.05049696210669 36.0 34.0 39.0 26.5 40.0 70-71 33.595537089699974 36.0 33.0 39.0 26.0 40.0 72-73 33.120179024044944 35.0 33.0 38.0 26.0 40.0 74-75 32.64458404838146 35.0 32.5 37.0 25.0 39.0 76-77 30.649591434528524 33.5 29.5 35.5 21.5 37.5 78-79 31.630332561052082 34.5 31.5 36.0 23.0 38.0 80-81 31.65392541660153 35.0 32.0 36.0 24.0 37.5 82-83 31.36148405347535 35.0 32.0 35.0 22.5 37.0 84-85 30.99535994512646 34.0 31.0 35.0 20.5 36.5 86-87 30.492306023618106 34.0 31.0 35.0 18.5 36.0 88-89 29.958369707838802 34.0 30.5 35.0 13.0 36.0 90-91 29.51216935794094 34.0 30.0 35.0 7.0 35.0 92-93 29.25851229297271 34.0 30.0 35.0 3.5 35.0 94-95 28.948047984486458 34.0 29.0 35.0 2.0 35.0 96-97 28.39586608502588 34.0 29.0 35.0 2.0 35.0 98-99 27.67762937174715 34.0 29.0 35.0 2.0 35.0 100-101 24.080461137976037 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06994957533827773 1101 2 0.07510693904761467 1101 3 0.13730714617888395 1101 4 0.09544061018561223 1101 5 0.09982111393407678 1101 6 0.13450168162135867 1101 7 0.05684992422925461 1101 8 0.14574776783292265 1101 9 0.14986365841419058 1101 10-11 0.19660854569640662 1101 12-13 0.1306052504701185 1101 14-15 0.19881738101011592 1101 16-17 0.19365808545347107 1101 18-19 0.24705541518182628 1101 20-21 0.2367347311168615 1101 22-23 0.2202306768338076 1101 24-25 0.20953369763478236 1101 26-27 0.1664799035009068 1101 28-29 0.10866914762092961 1101 30-31 0.16711210127652976 1101 32-33 0.18862818943647852 1101 34-35 0.24613131331027915 1101 36-37 0.32012583219418644 1101 38-39 0.37799773755899224 1101 40-41 0.2765216539306081 1101 42-43 0.2524836669744843 1101 44-45 0.17188980973458712 1101 46-47 0.06143132223625969 1101 48-49 0.07546056043424443 1101 50-51 0.1285084616013421 1101 52-53 0.033694601302904914 1101 54-55 0.0015264484828492186 1101 56-57 -0.033241148966141054 1101 58-59 0.13861013428902424 1101 60-61 0.09347439644182032 1101 62-63 0.017074281280635262 1101 64-65 -0.18525676542736136 1101 66-67 -0.11723904950336816 1101 68-69 -0.04780996303873053 1101 70-71 -0.007255397195613966 1101 72-73 -0.12440313441064887 1101 74-75 -0.08861756789352881 1101 76-77 -0.08781106510506831 1101 78-79 0.0032600443049481953 1101 80-81 0.024582523805872114 1101 82-83 0.1014144340274008 1101 84-85 -0.007647253386924291 1101 86-87 -0.044556303183799884 1101 88-89 0.18827153022310839 1101 90-91 0.192020134218712 1101 92-93 0.11620494371394585 1101 94-95 0.17139845311354662 1101 96-97 0.0767048310678966 1101 98-99 0.07256870781664304 1101 100-101 -0.09081110211085885 1102 1 0.19399734057008544 1102 2 0.22018634034960627 1102 3 0.24032819126907157 1102 4 0.1614139239380279 1102 5 0.1283673936498957 1102 6 0.18512151553024125 1102 7 0.2000392803642157 1102 8 0.2065805727039347 1102 9 0.2927935052674897 1102 10-11 0.23962576671163305 1102 12-13 0.28216966711628544 1102 14-15 0.35984948797450755 1102 16-17 0.39715864056530137 1102 18-19 0.3244942267483424 1102 20-21 0.30613690504396374 1102 22-23 0.2854748807599776 1102 24-25 0.28513847974659257 1102 26-27 0.23159085988963568 1102 28-29 0.2073420538946138 1102 30-31 0.339156664427712 1102 32-33 0.31532783939648823 1102 34-35 0.42679104127314105 1102 36-37 0.46427871032805257 1102 38-39 0.41263938059686467 1102 40-41 0.551916629385893 1102 42-43 0.43858778582404767 1102 44-45 0.4416363832676993 1102 46-47 0.5153910638860992 1102 48-49 0.5067396307131418 1102 50-51 0.4081683931871396 1102 52-53 0.3566969813885308 1102 54-55 0.3417479033027533 1102 56-57 0.4120608662799725 1102 58-59 0.3745551711028625 1102 60-61 0.3962726982213667 1102 62-63 0.498081849999501 1102 64-65 0.38609431544303163 1102 66-67 0.4441174311264504 1102 68-69 0.29724734245802864 1102 70-71 0.2631060555078477 1102 72-73 0.12948685280017713 1102 74-75 0.38520976968872134 1102 76-77 0.3792047359830093 1102 78-79 0.2650522573188603 1102 80-81 0.36802274753754105 1102 82-83 0.35427246433901516 1102 84-85 0.2411619922845354 1102 86-87 0.562130084118408 1102 88-89 0.5508520511925212 1102 90-91 0.6219625893938492 1102 92-93 0.66929076263132 1102 94-95 0.5660961584357089 1102 96-97 0.6149167319099931 1102 98-99 0.5257567780481835 1102 100-101 0.3914161343585043 1103 1 0.1348564596000017 1103 2 0.12872997976742795 1103 3 0.1597990977030932 1103 4 0.11717596095322591 1103 5 0.11071571192545093 1103 6 0.07202739334061903 1103 7 0.16935195286031757 1103 8 0.16131809308066636 1103 9 0.16710746275476396 1103 10-11 0.20051609799105563 1103 12-13 0.18731608782611175 1103 14-15 0.27324505543779054 1103 16-17 0.2557004797539335 1103 18-19 0.23253293291740817 1103 20-21 0.20234512575951413 1103 22-23 0.254887621168173 1103 24-25 0.20159555421277275 1103 26-27 0.21378808306715769 1103 28-29 0.25303584360052866 1103 30-31 0.2441683783328088 1103 32-33 0.2254939198505923 1103 34-35 0.23401827861660962 1103 36-37 0.34992815231908736 1103 38-39 0.307360363695814 1103 40-41 0.3605614540994466 1103 42-43 0.31123634508200837 1103 44-45 0.42117982031491863 1103 46-47 0.41648100954401457 1103 48-49 0.42486719371184734 1103 50-51 0.3615013623361776 1103 52-53 0.3428645169244433 1103 54-55 0.44963563852752486 1103 56-57 0.444394844798893 1103 58-59 0.43809394990187656 1103 60-61 0.3674774736707036 1103 62-63 0.32461437269169835 1103 64-65 0.24081979290725997 1103 66-67 0.3260005453491246 1103 68-69 0.2270947699305168 1103 70-71 0.3554494945415385 1103 72-73 0.3420258836312726 1103 74-75 0.23235677888727224 1103 76-77 0.25235884925839613 1103 78-79 0.37995852745504166 1103 80-81 0.3256559055244175 1103 82-83 0.32784224432618103 1103 84-85 0.25680685300266504 1103 86-87 0.36035493398550145 1103 88-89 0.4020088536378559 1103 90-91 0.646380401482201 1103 92-93 0.5663213777013496 1103 94-95 0.4505848871386888 1103 96-97 0.2387337640645697 1103 98-99 0.26339187283335264 1103 100-101 -0.3854648257968414 1104 1 0.011295475844839586 1104 2 0.04260130917273841 1104 3 0.10022752259753531 1104 4 0.010079279099258542 1104 5 0.036709843744823445 1104 6 0.09346175797886502 1104 7 0.08093087511884534 1104 8 0.03673971947316801 1104 9 0.08970353632171424 1104 10-11 0.07388684296374493 1104 12-13 0.09484572690914916 1104 14-15 0.10903081913617285 1104 16-17 0.12877483389625155 1104 18-19 0.06411853440592097 1104 20-21 0.1357007231927767 1104 22-23 0.21663948777441533 1104 24-25 0.18470246960123404 1104 26-27 0.17100642232432506 1104 28-29 0.12156653227685155 1104 30-31 0.11671912855346278 1104 32-33 0.08598687087752666 1104 34-35 0.18505475198318067 1104 36-37 0.24887498087756654 1104 38-39 0.22024889211832743 1104 40-41 0.2469233013836245 1104 42-43 0.10015180511396693 1104 44-45 0.1292581104320405 1104 46-47 0.20199960404318773 1104 48-49 0.11983391683391176 1104 50-51 0.15557772024748573 1104 52-53 0.09377158021013798 1104 54-55 0.13420780999290116 1104 56-57 0.1437293072401502 1104 58-59 0.23817266939807524 1104 60-61 0.17265566598481996 1104 62-63 0.1588740516115692 1104 64-65 0.12131167048652003 1104 66-67 -0.00322824213208861 1104 68-69 -0.05638129218632315 1104 70-71 -0.04886576511353269 1104 72-73 -0.09713527664639088 1104 74-75 -0.08934833310910761 1104 76-77 -0.003065043301422321 1104 78-79 -0.03249823645802863 1104 80-81 -0.01349630935595414 1104 82-83 0.06775958885517142 1104 84-85 0.0584021155202592 1104 86-87 0.009862340774464684 1104 88-89 0.10316161716925976 1104 90-91 0.16785293314891092 1104 92-93 0.3101198319366958 1104 94-95 0.09830142538920583 1104 96-97 0.013299017756040143 1104 98-99 0.15581989220311954 1104 100-101 -0.08098914535028356 1105 1 0.02564885167994646 1105 2 8.643849490397315E-4 1105 3 0.016117999515550707 1105 4 0.01614805048325252 1105 5 0.042541568214829795 1105 6 -0.0018572599431792014 1105 7 0.06912992044944133 1105 8 0.06981308827094068 1105 9 0.02950079639273895 1105 10-11 0.07505899828503004 1105 12-13 -0.023200163891850423 1105 14-15 0.07190137855763368 1105 16-17 0.05758043227766052 1105 18-19 0.0016719509834643986 1105 20-21 0.00933943962078132 1105 22-23 0.088706379450457 1105 24-25 -0.06572127490663604 1105 26-27 0.02938038235043905 1105 28-29 0.04417059911296661 1105 30-31 0.13025672965721213 1105 32-33 -4.4485544439964997E-4 1105 34-35 0.1499625490673182 1105 36-37 0.134734808370375 1105 38-39 0.1424604657487052 1105 40-41 0.1339545897243397 1105 42-43 0.14128465324189676 1105 44-45 0.03741747818347818 1105 46-47 0.055335275436902975 1105 48-49 -0.094834286933839 1105 50-51 -0.13244365564317917 1105 52-53 -0.15498288178132924 1105 54-55 -0.05676287860060114 1105 56-57 -0.06575303230177809 1105 58-59 -0.05753687405512409 1105 60-61 -0.04659293696623479 1105 62-63 -0.17049013485915765 1105 64-65 -0.14645874587341723 1105 66-67 -0.09702474291221108 1105 68-69 -0.16143289155851903 1105 70-71 -0.07455803932865734 1105 72-73 -0.014877566834407219 1105 74-75 -0.15847158361491864 1105 76-77 0.056503063703036815 1105 78-79 0.19880491969199454 1105 80-81 -0.0028518843567688634 1105 82-83 -0.04128997802492762 1105 84-85 0.017345659464218954 1105 86-87 0.12974140980974624 1105 88-89 0.2945359105425922 1105 90-91 0.29858777858228436 1105 92-93 0.24300117884466843 1105 94-95 0.06561314208110858 1105 96-97 -0.12017946051388861 1105 98-99 -0.21645730916175054 1105 100-101 -0.9061298919554694 1106 1 0.09109205467054693 1106 2 0.05212511869654435 1106 3 0.06892840100714892 1106 4 0.03892810018108861 1106 5 0.0486377216921241 1106 6 0.10888015834872533 1106 7 0.08060725052337858 1106 8 0.027562650587356075 1106 9 0.14599109989082848 1106 10-11 0.150528081983623 1106 12-13 0.1690251073992144 1106 14-15 0.08054029671361462 1106 16-17 0.1049421940441917 1106 18-19 0.11811760976422647 1106 20-21 0.22876591043272043 1106 22-23 0.14234452707168543 1106 24-25 0.1541546193931964 1106 26-27 0.172693893429269 1106 28-29 0.15012640282700573 1106 30-31 0.25241029822520744 1106 32-33 0.2213659739750753 1106 34-35 0.12481434514082679 1106 36-37 0.2210201496246711 1106 38-39 0.27720682092090954 1106 40-41 0.20627374058842918 1106 42-43 0.23525351570111752 1106 44-45 0.23917489267892478 1106 46-47 0.3428803252637138 1106 48-49 0.3617664179897133 1106 50-51 0.32186297221235094 1106 52-53 0.405410507625767 1106 54-55 0.3432461826235098 1106 56-57 0.38025265444310463 1106 58-59 0.3977658270494757 1106 60-61 0.3217888143898193 1106 62-63 0.36640988147750164 1106 64-65 0.5572802326686741 1106 66-67 0.507821226198935 1106 68-69 0.5630824156269014 1106 70-71 0.5690722838924742 1106 72-73 0.3416187686610499 1106 74-75 0.4665924898220126 1106 76-77 0.510700560027292 1106 78-79 0.6095267288679089 1106 80-81 0.49981853114335095 1106 82-83 0.6200365190447279 1106 84-85 0.568931472894274 1106 86-87 0.595807786914083 1106 88-89 0.828831982402729 1106 90-91 0.9090226049011072 1106 92-93 0.8978105392875975 1106 94-95 0.6658580596934165 1106 96-97 0.7220599978762436 1106 98-99 0.7779535029289661 1106 100-101 0.36506956939775037 1107 1 0.007129030233222977 1107 2 0.02036140254639207 1107 3 0.08958946929674028 1107 4 0.055724494583721196 1107 5 0.07545548896030851 1107 6 0.07386566976905584 1107 7 -0.017054871251424686 1107 8 0.05975773988096478 1107 9 0.04541736729417778 1107 10-11 0.03321448191895371 1107 12-13 -0.057159684518509835 1107 14-15 0.07680983482223525 1107 16-17 0.06471396417725117 1107 18-19 -0.0026277120793025688 1107 20-21 0.10356948722977677 1107 22-23 0.0373762200502199 1107 24-25 -0.05973888007248718 1107 26-27 0.10564663312241862 1107 28-29 -0.0418842254998637 1107 30-31 0.05014618922864855 1107 32-33 0.058828931464297796 1107 34-35 0.14821239076153603 1107 36-37 0.1822998310022399 1107 38-39 0.17032228239543912 1107 40-41 0.0686720236854228 1107 42-43 0.10776096209842478 1107 44-45 0.1338892137620178 1107 46-47 0.10813122906308337 1107 48-49 0.1711907527403227 1107 50-51 0.16377576788926973 1107 52-53 0.051732214726563086 1107 54-55 0.09377418840378482 1107 56-57 0.07100263739029344 1107 58-59 -0.004153625542421935 1107 60-61 0.19416962782187852 1107 62-63 -0.012260579757018775 1107 64-65 0.10867837429043448 1107 66-67 0.20029891227044772 1107 68-69 0.1434122423955273 1107 70-71 0.22485388402789397 1107 72-73 0.20149418203399705 1107 74-75 0.2585458964698333 1107 76-77 0.2724155303806519 1107 78-79 0.5306978658992954 1107 80-81 0.4001144922780817 1107 82-83 0.30623908378859355 1107 84-85 0.34549692161076706 1107 86-87 0.2985266377309763 1107 88-89 0.5156304484069913 1107 90-91 0.6100885955182669 1107 92-93 0.46125207591073547 1107 94-95 0.4209157992728798 1107 96-97 0.6089398121394751 1107 98-99 0.6952530186289252 1107 100-101 0.17841321021234435 1108 1 0.04556181353893862 1108 2 0.04930679922767922 1108 3 0.05542466764755005 1108 4 0.08703197556135933 1108 5 0.04820162448952203 1108 6 0.0831140819619236 1108 7 0.06671553106355077 1108 8 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2316 88-89 -0.5280360886449706 2316 90-91 -0.3722307375483247 2316 92-93 -0.33699950310320403 2316 94-95 0.06791406043211268 2316 96-97 -0.23020724169320772 2316 98-99 -0.1050975985437077 2316 100-101 0.2475457497025637 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 13.0 5 28.0 6 91.0 7 174.0 8 420.0 9 788.0 10 1419.0 11 2254.0 12 3468.0 13 5204.0 14 6303.0 15 8342.0 16 11085.0 17 16192.0 18 16553.0 19 10864.0 20 10019.0 21 10500.0 22 11136.0 23 12149.0 24 13911.0 25 16347.0 26 19459.0 27 23896.0 28 28841.0 29 35746.0 30 44283.0 31 54649.0 32 68776.0 33 89936.0 34 127000.0 35 188888.0 36 310983.0 37 549745.0 38 523859.0 39 53903.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.520992387436515 19.25385861123046 14.814202774013726 22.410946227319297 2 15.00867718585162 21.419650839877814 39.56941660855529 24.002255365715282 3 20.606823359546397 24.612293148360557 29.771134831875134 25.009748660217912 4 12.850007640841794 16.698752347143643 37.73653674893599 32.714703263078576 5 13.473834069020516 38.13443138963199 33.99515904368278 14.396575497664713 6 31.218503484048206 36.4946364803648 17.178983645085534 15.107876390501469 7 28.149212466340774 33.03834121715975 20.87666803089359 17.93577828560588 8 25.56700315645809 34.3706581136079 19.72355961349636 20.338779116437646 9 25.585358741913446 18.23961152906418 21.191611233969603 34.983418495052774 10-11 25.111626550783495 26.302236571000986 27.065969447171923 21.5201674310436 12-13 26.84346286463063 23.82111471977432 27.34024931978943 21.99517309580562 14-15 22.940318999233934 25.509641050660335 26.874202287837935 24.6758376622678 16-17 21.767733467240557 28.66623177557863 27.291779789948045 22.274254967232764 18-19 21.55520688695145 28.37317113656304 28.79236389409257 21.27925808239294 20-21 22.925862756429286 26.43867390116791 28.30530081203705 22.33016253036576 22-23 22.13378411372383 26.486055902857675 28.60259103579954 22.777568947618956 24-25 22.992226500335498 26.591477166936585 28.49309071044394 21.923205622283973 26-27 22.527237277194462 26.10202835926419 29.6295686388168 21.741165724724553 28-29 21.56612226400777 27.85378854014252 28.855199900229927 21.72488929561978 30-31 22.091340469264036 26.63273992643912 28.584511902208078 22.69140770208876 32-33 22.110005287111218 26.863245618811575 28.78878166338491 22.237967430692297 34-35 23.10502672143437 26.4607636941587 28.494811838853657 21.939397745553272 36-37 22.732565647585506 26.07894313778372 29.29879567394908 21.889695540681693 38-39 22.187619001187212 26.98664727070846 29.114997915900776 21.710735812203556 40-41 22.205706387894804 27.116728397921857 28.69369168570355 21.983873528479787 42-43 21.84720367624309 27.280891928562973 27.839390320195644 23.032514074998293 44-45 22.43523576589911 26.480882905421595 28.091087881919762 22.99279344675953 46-47 23.040819494569778 27.27233804019263 27.809113658235514 21.877728807002082 48-49 22.898248741887038 26.99332958607425 27.869902776192024 22.23851889584669 50-51 21.805820740582003 27.41975359602606 28.665474285493463 22.10895137789847 52-53 22.569556437668776 27.920676557761865 27.230716569029923 22.27905043553944 54-55 21.844705599243998 26.944220976657668 27.879894749599956 23.331178674498382 56-57 22.69900879838541 26.961961781738914 27.558672004131324 22.780357415744348 58-59 23.527772709277638 26.68021877476383 27.413464234577066 22.378544281381462 60-61 22.52459593799215 27.059638067950754 28.64226733222017 21.773498661836925 62-63 22.101297641637657 27.144859035338765 28.336380296083096 22.417463026940478 64-65 21.782837143227272 27.91510132027042 27.745835318606087 22.55622621789622 66-67 22.21974348102654 27.132898855868206 27.236164287550864 23.411193375554397 68-69 21.788666906146883 27.29193985123536 27.710532533153227 23.20886070946453 70-71 22.692009675766954 27.17832652793266 27.297593946695457 22.832069849604927 72-73 22.416363521391723 26.46519728302205 28.327652469924065 22.790786725662162 74-75 21.776003892438027 28.290655865268242 27.202801993293622 22.730538249000105 76-77 22.164121022910685 26.91674729418309 27.366537713983814 23.552593968922412 78-79 22.16081948899186 27.168627240886273 27.657731518726308 23.01282175139556 80-81 22.69407650969134 27.228886827356412 27.290103363286544 22.7869332996657 82-83 22.313681223966334 26.919210027843164 27.925151703645607 22.841957044544895 84-85 21.976663815256742 27.353642749368095 27.603880318069802 23.065813117305364 86-87 21.980134776494257 27.458699475849652 27.41588118848443 23.145284559171664 88-89 21.906245246456752 27.318395358116792 26.911414896970737 23.86394449845572 90-91 22.209860840774777 26.604409096820696 27.303798277538117 23.881931784866406 92-93 22.6144106276205 27.162094749951255 27.30766595996199 22.915828662466254 94-95 22.12660435721199 26.89066921478494 27.495942449530926 23.486783978472147 96-97 21.308902546771613 26.857339335931808 28.173232813814643 23.66052530348194 98-99 21.786626746331685 27.30486799150888 26.920712347872524 23.987792914286917 100-101 21.788252156798336 26.192090524490848 27.68167123217891 24.3379860865319 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 845.0 1 558.0 2 351.0 3 438.0 4 385.0 5 428.5 6 846.0 7 1675.0 8 2478.0 9 2701.0 10 2408.5 11 2141.5 12 2163.0 13 2352.5 14 2520.0 15 2618.5 16 2724.5 17 2897.0 18 3102.5 19 3244.0 20 3570.0 21 4063.0 22 4748.0 23 5855.5 24 7136.5 25 8822.5 26 11209.5 27 14032.5 28 16843.5 29 19894.5 30 23885.0 31 28721.5 32 33747.0 33 37984.0 34 41977.5 35 45483.0 36 49967.0 37 55304.0 38 58486.0 39 59899.0 40 61337.5 41 65046.5 42 68553.0 43 71408.5 44 74870.0 45 78205.5 46 81545.0 47 99219.0 48 139054.0 49 136241.0 50 99723.5 51 85583.0 52 79104.5 53 76263.0 54 73855.0 55 67389.5 56 58897.0 57 51708.5 58 46018.0 59 40556.0 60 34370.5 61 28081.0 62 22560.0 63 17490.0 64 13131.5 65 9766.5 66 7263.0 67 5775.0 68 4266.5 69 2904.0 70 2232.0 71 1737.5 72 1353.0 73 1129.0 74 897.5 75 679.0 76 433.0 77 223.0 78 127.0 79 66.5 80 34.0 81 17.0 82 11.0 83 8.0 84 4.5 85 3.5 86 3.0 87 3.0 88 2.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3495904684450799 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.002141192217231E-4 16-17 0.0016467331449177864 18-19 8.78257677289486E-5 20-21 0.0 22-23 0.0 24-25 5.269546063736917E-4 26-27 0.0011417349804763318 28-29 0.0 30-31 0.0032715098479033354 32-33 0.0 34-35 0.009880398869506719 36-37 0.026786859157329323 38-39 0.020770794067896343 40-41 0.013020170065816631 42-43 0.010429309917812647 44-45 0.0031836840801743867 46-47 0.0012076043062730433 48-49 7.026061418315888E-4 50-51 0.0 52-53 1.0978220966118577E-4 54-55 0.0 56-57 0.0 58-59 0.004369331944515192 60-61 0.010275614824286987 62-63 0.004610852805769802 64-65 0.0022395570770881892 66-67 0.004149767525192822 68-69 0.0036008564768868923 70-71 2.195644193223715E-5 72-73 0.0 74-75 0.0 76-77 0.2176102959904024 78-79 5.269546063736917E-4 80-81 0.003227596964038861 82-83 7.24562583763826E-4 84-85 0.0 86-87 0.11008959984823707 88-89 0.4036033155983833 90-91 0.39394248114819896 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0014930380513921264 100-101 7.904319095605374E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2277236.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.39724945043818 #Duplication Level Percentage of deduplicated Percentage of total 1 70.45928258017727 33.39576192551576 2 15.579503493311536 14.768512267729198 3 5.750626148309239 8.176915861428766 4 2.7731296761659916 5.257548760786094 5 1.559781477679244 3.69646758928681 6 0.9240331439180115 2.6277977653652784 7 0.5972449149924671 1.9815436353232594 8 0.43191524599902437 1.6377275724850502 9 0.2886763593459743 1.2314218872928928 >10 1.4781302909595813 12.499482990068907 >50 0.10188317092238325 3.2997834223964615 >100 0.049680058102122046 4.199537719818181 >500 0.0029640921780545574 0.951950215023839 >1k 0.002223069133540869 2.4662109200179048 >5k 7.410230445136231E-4 2.2935840581688214 >10k+ 1.8525576112840577E-4 1.5157534092928786 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 23351 1.0254097511193394 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11174 0.4906825643016358 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8634 0.3791438392858711 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7774 0.3413787591624232 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7499 0.3293027160996928 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6398 0.2809546309649066 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5944 0.2610181816904352 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5561 0.24419954717034162 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5289 0.2322552427592046 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5143 0.22584396171499133 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4886 0.21455835056182143 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4400 0.1932166890036869 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4246 0.1864541048885579 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3979 0.17472936489674326 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3645 0.16006246168600882 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3029 0.13301212522549266 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2653 0.11650088089245032 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2593 0.11386610786058186 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2588 0.1136465434412595 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2526 0.11092394464166208 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.39128838644743E-5 0.0 10-11 0.0 0.0 0.0 4.39128838644743E-5 0.0 12-13 0.0 0.0 0.0 4.39128838644743E-5 0.0 14-15 0.0 0.0 0.0 4.39128838644743E-5 0.0 16-17 0.0 0.0 0.0 6.586932579671146E-5 0.0 18-19 2.195644193223715E-5 0.0 0.0 1.317386515934229E-4 0.0 20-21 4.39128838644743E-5 0.0 0.0 1.9760797739013436E-4 0.0 22-23 4.39128838644743E-5 0.0 0.0 3.293466289835573E-4 0.0 24-25 8.78257677289486E-5 0.0 0.0 5.269546063736915E-4 0.0 26-27 8.78257677289486E-5 0.0 0.0 0.0011856478643408061 0.0 28-29 8.78257677289486E-5 0.0 0.0 0.003996072431667161 0.0 30-31 8.78257677289486E-5 0.0 0.0 0.011131916059644236 0.0 32-33 8.78257677289486E-5 0.0 0.0 0.024371650544783238 0.0 34-35 1.0978220966118577E-4 0.0 0.0 0.04498874951915392 0.0 36-37 1.317386515934229E-4 0.0 0.0 0.07542037803723461 0.0 38-39 1.317386515934229E-4 0.0 0.0 0.12616171534263468 0.0 40-41 1.317386515934229E-4 0.0 0.0 0.18619062758537103 0.0 42-43 1.9760797739013436E-4 0.0 0.0 0.24856887911485678 0.0 44-45 2.195644193223715E-4 0.0 0.0 0.32240839333297033 0.0 46-47 2.195644193223715E-4 0.0 0.0 0.4103219868296479 0.0 48-49 2.195644193223715E-4 0.0 0.0 0.5064033767251177 0.0 50-51 2.195644193223715E-4 0.0 0.0 0.6157903704315231 0.0 52-53 2.195644193223715E-4 0.0 0.0 0.721225204590126 0.0 54-55 2.634773031868458E-4 0.0 0.0 0.8390434719985105 0.0 56-57 2.634773031868458E-4 0.0 0.0 0.9922994366855258 0.0 58-59 2.634773031868458E-4 0.0 0.0 1.151000598971736 0.0 60-61 2.634773031868458E-4 0.0 0.0 1.337937745582803 0.0 62-63 2.634773031868458E-4 0.0 0.0 1.5255774983357018 0.0 64-65 2.634773031868458E-4 0.0 0.0 1.7167522382396907 0.0 66-67 2.634773031868458E-4 0.0 0.0 1.9425083741869529 0.0 68-69 2.634773031868458E-4 0.0 0.0 2.204536552206271 0.0 70-71 2.634773031868458E-4 0.0 0.0 2.4926270267991546 0.0 72-73 2.634773031868458E-4 0.0 0.0 2.7780607719182377 0.0 74-75 2.634773031868458E-4 0.0 0.0 3.0853850896437613 0.0 76-77 2.634773031868458E-4 0.0 0.0 3.4028313271000457 0.0 78-79 2.634773031868458E-4 0.0 0.0 3.793546211284206 0.0 80-81 2.634773031868458E-4 0.0 0.0 4.224309645552767 0.0 82-83 2.634773031868458E-4 4.39128838644743E-5 0.0 4.644446161926124 0.0 84-85 2.634773031868458E-4 4.39128838644743E-5 0.0 5.087395421467077 0.0 86-87 2.634773031868458E-4 4.39128838644743E-5 0.0 5.551861994101621 0.0 88-89 2.634773031868458E-4 4.39128838644743E-5 0.0 6.053193432740392 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9510 0.0 24.229723 1 TGCACCG 365 0.0 23.39976 5 TACATGG 9730 0.0 22.871347 2 ACATGGG 9475 0.0 22.535387 3 CATGGGG 4585 0.0 21.62911 4 GAGTACT 7650 0.0 21.18264 12-13 GTAAGCG 370 0.0 20.518707 94-95 GTACTTT 7945 0.0 20.366259 14-15 AGTACTT 8020 0.0 20.294136 12-13 GCACCGT 455 0.0 19.814081 6 ATCGATC 130 0.0076118875 18.249811 8 CGTACAC 235 2.55546E-6 18.172153 3 TAGTACT 565 0.0 17.6361 4 TACTTTT 9195 0.0 17.5976 14-15 ATGGGGA 2790 0.0 17.347132 5 ACTTTTT 9640 0.0 16.78471 16-17 AGTACAT 7550 0.0 16.528769 2 TACACTG 1810 0.0 16.253424 5 CATGGGA 5390 0.0 15.93388 4 AGAGTAC 16175 0.0 15.900316 10-11 >>END_MODULE