FastQCFastQC Report
Fri 27 May 2016
SRR939158_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939158_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238267
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT205890.9198634479264538No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT118270.5283998736522497No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT90100.40254357500691385No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC81300.363227443374718No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74710.33378502207288047No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT59890.26757308221047804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56150.2508637262667948No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT50910.2274527569767146No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC48800.21802582086944944No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC45390.2027908198619736No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC42370.1892982383245609No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG41240.18424968960360852No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA38350.1713379145562169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38060.17004226930924685No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA32010.14301242881211224No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31270.13970629956122305No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT25700.11482097533493547No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25590.114329523689533No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA25030.11182758804021146No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC24950.11147016866173695No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG23570.10530468438305171No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC22510.10056887761826448No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG94400.024.499461
TACATGG93500.023.8594742
ACATGGG93550.022.7834933
ATTCGCG1800.022.45642580-81
GAGTACT69150.020.811112-13
CATGGGG45700.020.5210974
GTACTTT72200.019.80149314-15
AGTACTT73200.018.9806312-13
TCGCGCT2150.018.80495360-61
AATTCGC2350.018.21226578-79
CAATTCG2101.8189894E-1218.11590478-79
CTCGCGC2300.017.57854360-61
TACACCG2504.6931145E-617.0500225
ATGGGGA27300.016.8279635
CATGGGA49100.016.7849224
ACTTTTT88250.016.57681516-17
TACTTTT84400.016.2100214-15
CGCGCTT2501.8189894E-1216.1733562-63
TACCTGG18550.016.0860062
CCTCGCG2553.6379788E-1215.85230658-59
Produced by FastQC (version 0.11.5)