FastQCFastQC Report
Fri 27 May 2016
SRR939158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939158_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238267
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT225481.0073865182303987No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT109700.4901113227331681No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT85290.3810537348761341No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73960.330434215399682No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC73430.32806631201728836No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT64730.28919695460818573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58100.2595758236171109No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC52930.23647759628319587No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC50910.2274527569767146No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG50290.22468275679353714No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT49480.22106388558648274No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA44010.19662533558328835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41430.18509856062748548No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC40390.18045210870731687No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA35420.1582474298195881No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29990.13398758950563092No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC25590.114329523689533No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25490.11388274946643989No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT24990.11164887835097423No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA23680.10579613602845417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG6050.027.45024794-95
GTACATG96750.022.5592461
TACATGG96200.022.0478422
ACATGGG94550.021.1277963
GTACCGT1353.8247535E-421.0887626
GAGTACT71700.020.35159112-13
CATGGGG45800.019.8915824
GTACTTT74400.019.26222414-15
AGTACTT76300.018.87585412-13
GGACCGT2253.572312E-516.871016
TACTTTT84900.016.68434114-15
ACTTTTT89150.016.10097516-17
CATGGGA51250.016.0171724
ATGGGGA24650.015.9769845
CCGATCG2104.0368913E-415.8169259
AGTACAT78550.015.6454182
AGAGTAC161600.015.1670610-11
TAGGACC7250.015.0530134
ATACAGT11700.015.0054656
TAGACAG13600.015.0024835
Produced by FastQC (version 0.11.5)