##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939157_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1595027 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.161246800210904 33.0 31.0 34.0 31.0 34.0 2 32.22948702435758 34.0 31.0 34.0 30.0 34.0 3 32.26569142716706 34.0 31.0 34.0 30.0 34.0 4 35.71974643689417 37.0 35.0 37.0 35.0 37.0 5 35.78258110991224 37.0 35.0 37.0 35.0 37.0 6 35.892947893672016 37.0 37.0 37.0 35.0 37.0 7 35.833809709804285 37.0 36.0 37.0 35.0 37.0 8 35.83095646656765 37.0 36.0 37.0 35.0 37.0 9 37.503969525280766 39.0 38.0 39.0 35.0 39.0 10-11 37.41968411819988 39.0 38.0 39.0 35.0 39.0 12-13 37.326668137906125 39.0 37.0 39.0 34.5 39.0 14-15 38.55477681569027 40.0 38.0 41.0 34.0 41.0 16-17 38.51914074181816 40.0 38.0 41.0 34.0 41.0 18-19 38.54483090254899 40.0 38.0 41.0 34.0 41.0 20-21 38.483458587221406 40.0 38.0 41.0 34.0 41.0 22-23 38.393577977049915 40.0 38.0 41.0 34.0 41.0 24-25 38.40778714090733 40.0 38.0 41.0 34.0 41.0 26-27 38.27741066452167 40.0 38.0 41.0 34.0 41.0 28-29 38.13986628439518 40.0 38.0 41.0 34.0 41.0 30-31 37.9282316224114 40.0 37.5 41.0 33.5 41.0 32-33 37.65307546518021 40.0 37.0 41.0 33.0 41.0 34-35 37.31251477247721 40.0 36.0 41.0 32.5 41.0 36-37 37.01365525473864 40.0 36.0 41.0 31.5 41.0 38-39 36.741947628472744 39.5 35.5 41.0 30.5 41.0 40-41 36.55894100852211 39.0 35.0 41.0 30.5 41.0 42-43 36.329166841689826 39.0 35.0 41.0 30.0 41.0 44-45 35.96084956555595 39.0 35.0 41.0 29.5 41.0 46-47 35.43218233923313 39.0 35.0 41.0 26.5 41.0 48-49 35.20918360629632 39.0 35.0 41.0 24.0 41.0 50-51 34.14368314768339 38.0 34.0 40.0 23.0 40.5 52-53 34.26943995305409 38.5 34.5 39.5 23.0 40.5 54-55 34.75994074081505 39.0 35.0 40.5 23.0 41.0 56-57 34.661719832955804 39.0 35.0 41.0 22.5 41.0 58-59 34.48041161685664 39.0 35.0 41.0 22.0 41.0 60-61 34.16930841923052 38.0 34.5 40.5 21.0 41.0 62-63 33.790368752378484 37.5 34.0 40.0 19.5 41.0 64-65 33.40029228345351 37.0 34.0 40.0 17.5 41.0 66-67 32.99775928557949 36.5 33.5 39.5 13.0 41.0 68-69 32.604980981513165 36.0 33.0 39.0 9.0 41.0 70-71 32.16794700027022 35.5 33.0 39.0 7.0 40.5 72-73 31.701980593431962 35.0 32.0 37.5 3.5 40.0 74-75 31.267768507993907 35.0 32.0 37.0 2.0 39.5 76-77 30.822658174438427 35.0 31.0 36.5 2.0 39.0 78-79 30.411150720332635 35.0 31.0 36.0 2.0 39.0 80-81 30.013318269847467 35.0 31.0 36.0 2.0 37.5 82-83 29.609517895308358 34.5 30.0 35.0 2.0 37.0 84-85 29.24550148680869 34.0 30.0 35.0 2.0 36.5 86-87 28.862187285857857 34.0 29.5 35.0 2.0 36.0 88-89 28.45359671027512 34.0 29.0 35.0 2.0 36.0 90-91 28.077498374635663 34.0 29.0 35.0 2.0 35.5 92-93 27.701501291200714 34.0 27.5 35.0 2.0 35.0 94-95 27.265177329286587 34.0 27.0 35.0 2.0 35.0 96-97 26.712599535932622 34.0 25.0 35.0 2.0 35.0 98-99 26.05098220907859 33.5 24.5 35.0 2.0 35.0 100-101 23.700823246252256 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.088985894475897 1101 2 0.1504257528561581 1101 3 0.15159664978991572 1101 4 0.09421965369669749 1101 5 0.20569924988208044 1101 6 0.19605982167932723 1101 7 0.14261442429758375 1101 8 0.29946049172507827 1101 9 0.3416904151747602 1101 10-11 0.2522407366827366 1101 12-13 0.19980651132086535 1101 14-15 0.28192393409058525 1101 16-17 0.38154348444471964 1101 18-19 0.431737310922955 1101 20-21 0.4561292104953196 1101 22-23 0.5068587429209614 1101 24-25 0.5447138600608028 1101 26-27 0.501477027971994 1101 28-29 0.4284175730051345 1101 30-31 0.47154559707939825 1101 32-33 0.5034191989446697 1101 34-35 0.23610632104694673 1101 36-37 0.21518502271842976 1101 38-39 0.16764410814924702 1101 40-41 0.16055839514017833 1101 42-43 0.1336219863485013 1101 44-45 0.05112840159306842 1101 46-47 0.15803235284933237 1101 48-49 0.11742564167715841 1101 50-51 -0.08509475080130358 1101 52-53 0.057175036717282524 1101 54-55 0.17113344478394765 1101 56-57 0.10779035813457227 1101 58-59 -0.0027730095407605404 1101 60-61 -0.07047569823522792 1101 62-63 0.028380624639090968 1101 64-65 -0.15433306889678278 1101 66-67 -0.1887705367176835 1101 68-69 -0.06307710036201541 1101 70-71 -0.11843151071436608 1101 72-73 -0.17998722927450928 1101 74-75 -0.2408407934278749 1101 76-77 -0.2894713615636597 1101 78-79 -0.420154705645313 1101 80-81 -0.3058743500126333 1101 82-83 -0.34896129013122845 1101 84-85 -0.42387084171398826 1101 86-87 -0.6665492073175123 1101 88-89 -0.6055222057855367 1101 90-91 -0.5290181027601619 1101 92-93 -0.6274612413816811 1101 94-95 -0.6627255752894108 1101 96-97 -0.9076964994392789 1101 98-99 -1.082516353321978 1101 100-101 -1.3050595127281852 1102 1 0.2963392336507411 1102 2 0.33637932622742994 1102 3 0.3740762915819218 1102 4 0.37025256981739574 1102 5 0.36809330547291097 1102 6 0.3129554595698423 1102 7 0.2742906973052399 1102 8 0.3680012657523193 1102 9 0.5020710245678046 1102 10-11 0.24645201187426125 1102 12-13 0.4089492827167476 1102 14-15 0.5132708851156735 1102 16-17 0.4928168002535216 1102 18-19 0.5951948969847862 1102 20-21 0.5944235423522031 1102 22-23 0.5354805478412317 1102 24-25 0.41780217140807707 1102 26-27 0.42420078068843026 1102 28-29 0.4776425007231211 1102 30-31 0.4668828372593623 1102 32-33 0.5408094361154454 1102 34-35 0.5007825860158235 1102 36-37 0.6357983279696313 1102 38-39 0.732461051751855 1102 40-41 0.6404212535692366 1102 42-43 0.5707169323681001 1102 44-45 0.564583977960126 1102 46-47 0.5821165349437507 1102 48-49 0.6962514976061627 1102 50-51 0.5186188971698371 1102 52-53 0.5635720291420654 1102 54-55 0.6305518123222953 1102 56-57 0.6629031556028977 1102 58-59 0.578033601551148 1102 60-61 0.5276973552414148 1102 62-63 0.3712937709904196 1102 64-65 0.3419425647599752 1102 66-67 0.25073336342996555 1102 68-69 0.2279299174921121 1102 70-71 0.08442091605905233 1102 72-73 -0.11464294176489531 1102 74-75 -0.11881212714170886 1102 76-77 -0.08478689704012865 1102 78-79 -0.0643682584909051 1102 80-81 -0.30845747768720244 1102 82-83 -0.3197818619027828 1102 84-85 -0.3113839382248891 1102 86-87 -0.40536257634302686 1102 88-89 -0.2640983772366141 1102 90-91 -0.30934051139273677 1102 92-93 -0.39099599972258403 1102 94-95 -0.3033634792045632 1102 96-97 -0.24177636738893682 1102 98-99 -0.1593183126403943 1102 100-101 -0.5141390457287116 1103 1 0.2241491575361536 1103 2 0.2761595326671866 1103 3 0.29010849559789875 1103 4 0.2920454330675426 1103 5 0.4244969043020461 1103 6 0.32956451441148715 1103 7 0.3293269964178833 1103 8 0.317322743922098 1103 9 0.47471247180196485 1103 10-11 0.34393059189190467 1103 12-13 0.40854741276563544 1103 14-15 0.5516994375570832 1103 16-17 0.4815615636473467 1103 18-19 0.5323221338253674 1103 20-21 0.5629230604222215 1103 22-23 0.590606325810171 1103 24-25 0.4611612181390612 1103 26-27 0.6144924817599744 1103 28-29 0.6566313477854351 1103 30-31 0.6050070569357118 1103 32-33 0.677672056244738 1103 34-35 0.724236145442859 1103 36-37 0.6464392477494769 1103 38-39 0.7010694096003078 1103 40-41 0.574933719386749 1103 42-43 0.49638668945654985 1103 44-45 0.4257687826712129 1103 46-47 0.3482546484364306 1103 48-49 0.41442606614538846 1103 50-51 0.3822251253168574 1103 52-53 0.3705064835251193 1103 54-55 0.2898108332038305 1103 56-57 0.3152987295914471 1103 58-59 0.26252278193528866 1103 60-61 0.4771582843772961 1103 62-63 0.3315476293082398 1103 64-65 0.28074923077778635 1103 66-67 0.24004220990622116 1103 68-69 0.18142131967288577 1103 70-71 0.3187558369879966 1103 72-73 0.19778321201524918 1103 74-75 0.15931484492519132 1103 76-77 0.09841164241376532 1103 78-79 0.1450750574385289 1103 80-81 0.2597452950061516 1103 82-83 0.2089118095873701 1103 84-85 0.026378336861370144 1103 86-87 0.13813252011099664 1103 88-89 0.09135559452574071 1103 90-91 0.13778297884778468 1103 92-93 -0.09645855308285434 1103 94-95 -0.011193559379709228 1103 96-97 -0.03275977664847929 1103 98-99 0.05889523937716845 1103 100-101 -0.04353834434006387 1104 1 0.18345149488209245 1104 2 0.29071153967659313 1104 3 0.27583775521914333 1104 4 0.3044434233264042 1104 5 0.3194337002501939 1104 6 0.29436976256351954 1104 7 0.18224377184773033 1104 8 0.2934727500937697 1104 9 0.29988502584461685 1104 10-11 0.3769768916169127 1104 12-13 0.4052556826116245 1104 14-15 0.4316102214006392 1104 16-17 0.2805606389074242 1104 18-19 0.43929724274030946 1104 20-21 0.3724549639497923 1104 22-23 0.3637096477916657 1104 24-25 0.4222154014237347 1104 26-27 0.45968251885074807 1104 28-29 0.3812935748910462 1104 30-31 0.45923101397273314 1104 32-33 0.2848938299242505 1104 34-35 0.30852861822172173 1104 36-37 0.3437002368772113 1104 38-39 0.3826841835853685 1104 40-41 0.3091276476698255 1104 42-43 0.2087389485941813 1104 44-45 0.21230056276522902 1104 46-47 0.3657518610638846 1104 48-49 0.3916449866885827 1104 50-51 0.2812600165435626 1104 52-53 0.39910917518606936 1104 54-55 0.4682411225847005 1104 56-57 0.2381370741248574 1104 58-59 0.22996009893492442 1104 60-61 0.30768046902985446 1104 62-63 0.23377358151719108 1104 64-65 0.22156182828523896 1104 66-67 0.35712399779071546 1104 68-69 0.32713615173242516 1104 70-71 0.1206647065996691 1104 72-73 0.13311616897740564 1104 74-75 0.11730848303823294 1104 76-77 0.2081875801546893 1104 78-79 0.22699649299103797 1104 80-81 0.33237683774350657 1104 82-83 0.12240562678024602 1104 84-85 0.08610449647916951 1104 86-87 0.07162763234447667 1104 88-89 0.04832938816506882 1104 90-91 -0.017644657723348445 1104 92-93 0.05947344109755903 1104 94-95 1.63845520802397E-4 1104 96-97 -0.08615187244911127 1104 98-99 0.08105308054448557 1104 100-101 -0.16565864956494636 1105 1 0.19427991458473315 1105 2 0.20785501769282888 1105 3 0.38995719185416533 1105 4 0.32856144359404027 1105 5 0.296686451980392 1105 6 0.3089059604513906 1105 7 0.33470504972154913 1105 8 0.2691645873550996 1105 9 0.44743364314020795 1105 10-11 0.35326049881360433 1105 12-13 0.45787567124187945 1105 14-15 0.4908982930649515 1105 16-17 0.3415187629385201 1105 18-19 0.3947093183394017 1105 20-21 0.3880553776066762 1105 22-23 0.5273384004376567 1105 24-25 0.38493680583930256 1105 26-27 0.4036695542385971 1105 28-29 0.3792112237987908 1105 30-31 0.3661679157757902 1105 32-33 0.36828828048042084 1105 34-35 0.28053644510670495 1105 36-37 0.4073969945600311 1105 38-39 0.4374461853587377 1105 40-41 0.3783036128853965 1105 42-43 0.22752376358361204 1105 44-45 0.17737012968665056 1105 46-47 0.2352772615757175 1105 48-49 0.4383138070097701 1105 50-51 0.504143856955821 1105 52-53 0.4833602369765515 1105 54-55 0.6345478767169013 1105 56-57 0.6028332439182051 1105 58-59 0.6081753014075559 1105 60-61 0.5928776836366723 1105 62-63 0.6385970885592585 1105 64-65 0.4811324943465536 1105 66-67 0.5554236778468962 1105 68-69 0.5079150761857179 1105 70-71 0.39985788164909764 1105 72-73 0.5507345181139023 1105 74-75 0.4191819684467397 1105 76-77 0.4841022527506986 1105 78-79 0.3065211562157373 1105 80-81 0.3640860051329078 1105 82-83 0.36746871333581765 1105 84-85 0.2438495037841335 1105 86-87 0.38812074550058995 1105 88-89 0.12769204224352748 1105 90-91 0.22281038425350985 1105 92-93 0.36072896347518935 1105 94-95 0.03602704383358102 1105 96-97 0.11359715889141242 1105 98-99 0.08178066653059801 1105 100-101 -0.028821553156888058 1106 1 0.046790147670932924 1106 2 0.11041459500932405 1106 3 0.16866962357632076 1106 4 0.16184941872125336 1106 5 0.0911478026652972 1106 6 0.13430848016846397 1106 7 0.009078487956038828 1106 8 -0.005225069874313704 1106 9 0.09376746304339179 1106 10-11 0.14952174558699483 1106 12-13 0.15289877643718341 1106 14-15 0.06688359700616786 1106 16-17 0.20071589520030386 1106 18-19 0.2196266427000566 1106 20-21 0.26988419298778155 1106 22-23 0.26959520485581834 1106 24-25 0.17883117894766087 1106 26-27 0.18383001232898977 1106 28-29 0.1219188612960096 1106 30-31 0.14911171833289671 1106 32-33 0.02407516288720757 1106 34-35 0.17239354751453106 1106 36-37 0.17448604234009224 1106 38-39 0.16090682405726398 1106 40-41 0.22447744202515452 1106 42-43 0.23053596081758343 1106 44-45 -0.025031243704859207 1106 46-47 -0.1998680633677168 1106 48-49 -0.2126836611776426 1106 50-51 -0.2662886404120286 1106 52-53 -0.2516763657212948 1106 54-55 -0.29336625057746346 1106 56-57 -0.1404482436110399 1106 58-59 -0.23822119528954744 1106 60-61 -0.28632791549024716 1106 62-63 -0.18316921496685978 1106 64-65 -0.25922270164213757 1106 66-67 -0.4426644050404107 1106 68-69 -0.3825592881808788 1106 70-71 -0.4255446380679757 1106 72-73 -0.3252829489189253 1106 74-75 -0.20766088048844367 1106 76-77 -0.2079471409612843 1106 78-79 -0.14375869017113985 1106 80-81 0.02241225983710038 1106 82-83 0.10336093674009916 1106 84-85 0.1568194766516804 1106 86-87 -0.0053733758009997246 1106 88-89 0.11087102022693074 1106 90-91 0.013568168683825377 1106 92-93 -0.16429021067558125 1106 94-95 -0.11304819855792303 1106 96-97 -0.029843357257480108 1106 98-99 0.04391893483675702 1106 100-101 0.007182553822499216 1107 1 0.13986301214988828 1107 2 0.11557856879021955 1107 3 0.0517182729584178 1107 4 0.0667542805282011 1107 5 0.10307791288573753 1107 6 0.0766501948478222 1107 7 0.10037827503018804 1107 8 0.15163683101079073 1107 9 0.0568890186546156 1107 10-11 0.15730097592265935 1107 12-13 0.2270157604051164 1107 14-15 0.2649868347316158 1107 16-17 0.2903704647560801 1107 18-19 0.14262759251635515 1107 20-21 0.14371649300447586 1107 22-23 0.13822178092462423 1107 24-25 0.31424654092160864 1107 26-27 0.22092242632363934 1107 28-29 0.09090827240990507 1107 30-31 0.05570811424972533 1107 32-33 0.15958120100510342 1107 34-35 0.1462396772740533 1107 36-37 0.3733244744125699 1107 38-39 0.47425683136354024 1107 40-41 0.35613202723724413 1107 42-43 0.3837858199107771 1107 44-45 0.6614263068128423 1107 46-47 0.5895577935544623 1107 48-49 0.49457264831634973 1107 50-51 0.3630794708104119 1107 52-53 0.4067329007282652 1107 54-55 0.3893168239048279 1107 56-57 0.4590280527316466 1107 58-59 0.4971003592779226 1107 60-61 0.49695218638015604 1107 62-63 0.4690864682746181 1107 64-65 0.42213284799881734 1107 66-67 0.18935875586473827 1107 68-69 0.4421076237680239 1107 70-71 0.43094177225266606 1107 72-73 0.45516401271266815 1107 74-75 0.527406258998866 1107 76-77 0.40337576526784247 1107 78-79 0.4864444243913191 1107 80-81 0.5486750719196003 1107 82-83 0.6856726956746328 1107 84-85 0.5436099847512104 1107 86-87 0.3470260845780899 1107 88-89 0.35662294073461354 1107 90-91 0.3660583052068702 1107 92-93 0.5137840367776576 1107 94-95 0.3717488695365958 1107 96-97 0.5084793819709752 1107 98-99 0.4028366400388279 1107 100-101 0.39487395224434607 1108 1 -0.020058325908529184 1108 2 0.07507975006169687 1108 3 0.022488515656007735 1108 4 0.00434153353506872 1108 5 0.17175274106443084 1108 6 0.11291388685813075 1108 7 0.20971300441163265 1108 8 0.27401252903484874 1108 9 0.1995946672800457 1108 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-0.9281171376744766 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5304.0 3 1180.0 4 1122.0 5 1256.0 6 1482.0 7 1821.0 8 2260.0 9 2752.0 10 3433.0 11 4586.0 12 6622.0 13 10696.0 14 9684.0 15 10976.0 16 14668.0 17 22216.0 18 21299.0 19 10990.0 20 10183.0 21 10340.0 22 10526.0 23 11093.0 24 11998.0 25 13467.0 26 15413.0 27 18128.0 28 20836.0 29 25065.0 30 30066.0 31 37327.0 32 46372.0 33 60870.0 34 85608.0 35 136431.0 36 209416.0 37 328893.0 38 318851.0 39 61721.0 40 76.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.923824592417375 18.349978453089072 14.453164564925402 20.27303238956815 2 15.022824056404795 21.51820149448607 40.62663208324126 22.832342365867866 3 20.903239170074475 27.31768095383061 29.616641193104247 22.162438682990672 4 12.333424353988745 16.6869109158875 37.64457375654799 33.33509097357576 5 12.827565682656363 39.129069888631754 32.645815308770956 15.397549119940932 6 28.980213223523236 36.025726386363935 18.411849349153048 16.582211040959777 7 27.184186347752743 35.22675924810218 20.1466291119412 17.442425292203882 8 26.090091922079 32.84943381873915 19.82130237380641 21.23917188537544 9 27.751162843236433 17.909417461349424 20.824934672433397 33.51448502298074 10-11 24.928045353155888 26.79508527719914 26.03758867451273 22.239280695132248 12-13 28.155703548997657 24.67674199103337 26.753820607180334 20.41373385278864 14-15 24.45301637616897 25.129381744630418 27.913540706024403 22.504061173176208 16-17 20.711461954543744 28.92665754696854 28.485711775705624 21.876168722782094 18-19 20.44536394850917 27.629064417514815 31.76476162802009 20.160810005955927 20-21 22.56004463942461 25.649153078014486 30.940167667737565 20.850634614823342 22-23 21.465337746606718 25.932458838986033 30.72175600239286 21.88044741201439 24-25 21.660328436965873 25.985161793580364 31.469585129775453 20.884924639678314 26-27 21.910819868086833 25.257146552189575 32.14915755625395 20.682876023469646 28-29 20.703077549398454 26.236715204814747 32.43914787724204 20.621059368544756 30-31 20.800351264719332 26.618879348791868 31.746051175051416 20.834718211437384 32-33 20.709129938112753 26.640357263905802 31.336726880154654 21.31378591782679 34-35 21.60567199923756 25.952680287626045 31.237609172177113 21.20403854095928 36-37 21.967099177573957 25.672206637378924 31.793206352566962 20.567487832480158 38-39 21.991744111553395 25.60623078313773 31.875597254252515 20.526427851056365 40-41 21.107403726614194 26.219689612563624 32.065496006479314 20.607410654342868 42-43 20.731069624268482 26.99346112871288 30.819856899583375 21.455612347435263 44-45 21.059066511335146 27.0502405895373 30.26740501643519 21.62328788269236 46-47 21.515527292799298 27.01589527993487 29.460709811898084 22.00786761536775 48-49 21.89650592732936 26.84412366807208 29.557968660445304 21.701401744153255 50-51 21.97249712356509 27.584128632404852 29.56272518421155 20.880649059818506 52-53 21.57845138552598 27.944551865027517 29.351108612974297 21.125888136472206 54-55 20.598083253082983 27.442469398549225 29.797372198758776 22.162075149609016 56-57 21.661556679910117 28.092266361799663 28.662632217954716 21.583544740335512 58-59 21.791696802851256 27.20912533640484 29.046800222050024 21.952377638693886 60-61 21.407422585273995 26.945074203505605 30.03867313411649 21.608830077103914 62-63 22.081288705401285 26.854685816321933 29.658782978238197 21.405242500038586 64-65 21.49283321207775 27.415882286402432 29.879507974353263 21.211776527166553 66-67 21.091640255388498 27.407722281707557 29.542230420624684 21.958407042279262 68-69 20.794749280739577 28.111492692679697 29.384652184916032 21.709105841664694 70-71 21.276324198409487 27.974301960472893 28.589557421952307 22.159816419165317 72-73 21.20319146153902 27.420119719195245 28.714425573789708 22.662263245476026 74-75 20.994158275503267 28.32177572290474 28.51053357022075 22.17353243137124 76-77 21.549474244005225 27.239976358623053 28.897204117329782 22.31334528004194 78-79 21.213930893590643 27.349509881276017 29.41918702751601 22.01737219761733 80-81 21.13267251635322 28.350831260471278 28.774422735346363 21.742073487829146 82-83 20.986686290804776 28.01511629885059 28.57432232772857 22.423875082616068 84-85 21.019104267154162 27.602725247667454 28.493940586274153 22.884229898904238 86-87 21.360143027038095 27.601761059786245 28.713749025352648 22.324346887823012 88-89 21.600387676402374 27.00967710122406 28.678916409166227 22.71101881320734 90-91 20.9812513491497 27.10469716808554 29.21165539289603 22.70239608986873 92-93 20.95876347782575 27.752835471740443 28.86521890654807 22.423182143885736 94-95 20.665745259194697 27.777788281738925 28.613640990153797 22.94282546891258 96-97 20.41306302928014 27.22232270986389 28.56314997034739 23.80146429050858 98-99 20.96064612772694 27.292323515084068 28.13480152280649 23.612228834382506 100-101 21.416270692174542 26.535289881053277 28.853229305620054 23.195210121152126 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4762.0 1 2976.5 2 1726.0 3 2083.5 4 1523.5 5 1362.0 6 2487.0 7 5150.5 8 7901.0 9 8411.0 10 6554.0 11 4887.0 12 4731.0 13 4963.5 14 5125.0 15 5248.0 16 5347.0 17 5433.5 18 5454.0 19 5557.5 20 5854.5 21 6350.0 22 6990.5 23 7840.0 24 8847.5 25 9930.5 26 11564.0 27 13539.0 28 15342.5 29 17053.0 30 18992.0 31 21122.0 32 23404.5 33 25738.5 34 27803.0 35 29221.0 36 31100.0 37 33633.0 38 35438.5 39 36118.5 40 36886.0 41 39293.0 42 42156.0 43 44241.5 44 46159.5 45 48319.0 46 51287.0 47 60976.0 48 98257.0 49 99067.5 50 66224.5 51 57139.0 52 50203.0 53 47446.0 54 45994.0 55 42747.5 56 37732.5 57 33169.5 58 30084.5 59 27124.5 60 23528.5 61 20217.5 62 16850.0 63 12896.5 64 9420.0 65 7261.0 66 5692.5 67 4740.0 68 3667.0 69 2586.5 70 1988.5 71 1482.5 72 1189.5 73 1007.5 74 777.0 75 605.0 76 442.5 77 275.5 78 188.0 79 139.0 80 94.5 81 64.5 82 47.0 83 36.0 84 28.0 85 20.5 86 17.0 87 15.5 88 13.5 89 8.0 90 6.5 91 6.0 92 4.0 93 1.5 94 2.5 95 4.0 96 4.5 97 3.5 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3430662929216872 2 0.12357157590435774 3 0.1173647844205772 4 0.12426121940255558 5 0.14375932194251256 6 0.09328995684712547 7 0.10150298396202698 8 0.1078978600362251 9 0.09523349761477393 10-11 0.09332130427886173 12-13 0.09799207160756526 14-15 0.09927731630875213 16-17 0.0882116729058505 18-19 0.10382269391051062 20-21 0.11388521949785176 22-23 0.12250576322532471 24-25 0.13664345493838034 26-27 0.13548359996413853 28-29 0.13087552749890755 30-31 0.12078165447983014 32-33 0.11328961829486271 34-35 0.33814474613909357 36-37 0.5024993307323324 38-39 0.47359699867149585 40-41 0.4517164913195827 42-43 0.4858851919121119 44-45 0.494286303617431 46-47 0.5024993307323324 48-49 0.5130007203639814 50-51 0.5011200437359368 52-53 0.5057281162011678 54-55 0.4894274516983098 56-57 0.4670453854386164 58-59 0.4449140986328131 60-61 0.40478938601039355 62-63 0.4787066300445071 64-65 0.5114960436406406 66-67 0.5183611311908827 68-69 0.5330003818117186 70-71 0.5246933124016082 72-73 0.5160100738106628 74-75 0.5227497716339599 76-77 0.5002423156473214 78-79 0.4768257841403311 80-81 0.47952166326965 82-83 0.4931577960749254 84-85 0.49331453323360674 86-87 0.4961984969533431 88-89 0.5101167566442448 90-91 0.5253516084680698 92-93 0.5174834031022671 94-95 0.5218720435453443 96-97 0.5216212640914543 98-99 0.5153517777442012 100-101 0.5024366358688599 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1595027.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.88871192824792 #Duplication Level Percentage of deduplicated Percentage of total 1 76.31028833763057 39.59642568712849 2 13.473252866530617 13.982194734556955 3 4.566122031017653 7.107905720901039 4 1.9617412445270197 4.071689052601004 5 1.0761374493227798 2.791969305155461 6 0.6206331882360198 1.9322314030493337 7 0.41087357382642253 1.4923790357846263 8 0.2695883183734818 1.1190872473041906 9 0.19653983134723277 0.9178378822082692 >10 0.990309294765281 9.325746299397634 >50 0.07046617515999987 2.511769786964737 >100 0.04435319791217751 4.292760514839564 >500 0.004726064533473461 1.6861722104482149 >1k 0.004120158824053787 4.543220492299928 >5k 4.847245675357396E-4 2.0703649388879 >10k+ 3.6354342565180474E-4 2.5582456884727636 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16362 1.025813356137545 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12644 0.7927138537466764 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11679 0.7322133104956844 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9609 0.6024349431075462 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8809 0.5522790523295217 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7845 0.49184120394200226 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6663 0.4177358753174711 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4427 0.2775501605928928 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4099 0.25698624537390274 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3476 0.21792734543051623 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3413 0.2139775690317468 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3380 0.2119086385371533 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3077 0.19291209490497652 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3064 0.19209706167983362 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2997 0.18789650582717407 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2797 0.17535753313266797 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2721 0.17059272350875565 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2707 0.1697149954201402 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2122 0.13303850028870984 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2113 0.13247424651745707 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2108 0.13216077220009442 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1919 0.12031144300378614 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1815 0.11379117720264297 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1799 0.11278805938708249 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1770 0.11096990834637908 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1735 0.1087755881248405 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1635 0.10250610177758746 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.269486347253056E-5 7 0.0 0.0 0.0 0.0 6.269486347253056E-5 8 0.0 0.0 0.0 0.0 1.2538972694506111E-4 9 0.0 0.0 0.0 6.269486347253056E-5 1.2538972694506111E-4 10-11 0.0 0.0 0.0 1.2538972694506111E-4 1.2538972694506111E-4 12-13 0.0 0.0 0.0 1.8808459041759167E-4 1.2538972694506111E-4 14-15 0.0 0.0 0.0 2.5077945389012223E-4 1.2538972694506111E-4 16-17 0.0 0.0 0.0 2.821268856263875E-4 1.2538972694506111E-4 18-19 0.0 0.0 0.0 3.134743173626528E-4 1.2538972694506111E-4 20-21 0.0 0.0 0.0 5.015589077802445E-4 1.2538972694506111E-4 22-23 0.0 0.0 0.0 0.001003117815560489 1.5673715868132638E-4 24-25 0.0 0.0 0.0 0.0018181510407033862 1.8808459041759167E-4 26-27 0.0 0.0 0.0 0.0024450996754286918 1.8808459041759167E-4 28-29 0.0 3.134743173626528E-5 0.0 0.006614308096351974 1.8808459041759167E-4 30-31 0.0 6.269486347253056E-5 0.0 0.016520096525011803 1.8808459041759167E-4 32-33 0.0 6.269486347253056E-5 0.0 0.03197438037099058 1.8808459041759167E-4 34-35 0.0 6.269486347253056E-5 0.0 0.057585232099519316 1.8808459041759167E-4 36-37 0.0 6.269486347253056E-5 0.0 0.09683221663332345 1.8808459041759167E-4 38-39 0.0 6.269486347253056E-5 0.0 0.15253660282866685 2.1943202215385696E-4 40-41 0.0 6.269486347253056E-5 0.0 0.220027623356846 2.5077945389012223E-4 42-43 0.0 6.269486347253056E-5 0.0 0.2847600698922338 2.5077945389012223E-4 44-45 0.0 6.269486347253056E-5 0.0 0.3623136160077541 2.5077945389012223E-4 46-47 0.0 6.269486347253056E-5 0.0 0.4567634278291214 2.5077945389012223E-4 48-49 0.0 6.269486347253056E-5 0.0 0.5622475356216541 2.5077945389012223E-4 50-51 0.0 6.269486347253056E-5 0.0 0.6823395466032863 2.5077945389012223E-4 52-53 0.0 6.269486347253056E-5 0.0 0.7959739866472479 2.821268856263875E-4 54-55 0.0 6.269486347253056E-5 0.0 0.9182916652821551 3.134743173626528E-4 56-57 0.0 6.269486347253056E-5 0.0 1.0693862862509538 3.134743173626528E-4 58-59 6.269486347253056E-5 6.269486347253056E-5 0.0 1.251765644092545 3.448217490989181E-4 60-61 6.269486347253056E-5 6.269486347253056E-5 0.0 1.4708841919290396 3.7616918083518334E-4 62-63 6.269486347253056E-5 6.269486347253056E-5 0.0 1.690849120422413 4.3886404430771393E-4 64-65 6.269486347253056E-5 6.269486347253056E-5 0.0 1.8971779161105111 4.3886404430771393E-4 66-67 6.269486347253056E-5 6.269486347253056E-5 0.0 2.1360139985091164 4.3886404430771393E-4 68-69 6.269486347253056E-5 6.269486347253056E-5 0.0 2.417482588069042 4.3886404430771393E-4 70-71 6.269486347253056E-5 6.269486347253056E-5 0.0 2.7317719386568378 4.3886404430771393E-4 72-73 6.269486347253056E-5 6.269486347253056E-5 0.0 3.064023367629513 4.3886404430771393E-4 74-75 6.269486347253056E-5 6.269486347253056E-5 0.0 3.4054595941009147 4.3886404430771393E-4 76-77 6.269486347253056E-5 6.269486347253056E-5 0.0 3.7584003280195257 4.3886404430771393E-4 78-79 6.269486347253056E-5 6.269486347253056E-5 0.0 4.155979804730578 4.3886404430771393E-4 80-81 6.269486347253056E-5 6.269486347253056E-5 0.0 4.607602253754952 4.702114760439792E-4 82-83 6.269486347253056E-5 6.269486347253056E-5 0.0 5.074083385422316 5.015589077802445E-4 84-85 6.269486347253056E-5 6.269486347253056E-5 0.0 5.581566957800715 5.015589077802445E-4 86-87 6.269486347253056E-5 6.269486347253056E-5 0.0 6.11325074747951 5.015589077802445E-4 88-89 6.269486347253056E-5 6.269486347253056E-5 0.0 6.652928132251052 5.015589077802445E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1615 0.0 29.921215 2 GTGTAGC 755 0.0 23.890995 1 GAGTACT 11265 0.0 22.97599 12-13 AGTACTT 11385 0.0 22.068235 12-13 CATGGGG 4135 0.0 21.768557 4 GTACATG 16225 0.0 21.590826 1 TACATGG 16135 0.0 21.551556 2 GTACCTG 2330 0.0 21.390995 1 GTACTTT 12180 0.0 21.290182 14-15 TACCGGC 115 0.0037886344 20.590826 7 ACATGGG 16245 0.0 20.472408 3 GTACCCG 190 8.303277E-6 19.986372 1 ACTTTTT 13685 0.0 19.434866 16-17 TACTTTT 12990 0.0 19.21516 14-15 ATGGGAG 2940 0.0 18.853418 5 ACCTGGG 2545 0.0 18.801182 3 GTACACG 305 4.603862E-9 18.67579 1 ATGGGAT 3285 0.0 18.459764 5 ATGGGGA 1905 0.0 17.90561 5 CATGGGA 11210 0.0 17.792133 4 >>END_MODULE