##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939157_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1595027 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4626667761737 34.0 31.0 34.0 31.0 34.0 2 32.648335106553056 34.0 31.0 34.0 31.0 34.0 3 32.72509744349155 34.0 31.0 34.0 31.0 34.0 4 36.194676328363094 37.0 35.0 37.0 35.0 37.0 5 36.176294821341585 37.0 35.0 37.0 35.0 37.0 6 36.239093758287474 37.0 37.0 37.0 35.0 37.0 7 36.196503256684686 37.0 37.0 37.0 35.0 37.0 8 36.19479105996325 37.0 37.0 37.0 35.0 37.0 9 37.90929119068204 39.0 38.0 39.0 35.0 39.0 10-11 37.851272737075924 39.0 38.0 39.0 35.0 39.0 12-13 37.777234178480995 39.0 38.0 39.0 35.0 39.0 14-15 39.02917536819126 40.0 38.0 41.0 36.0 41.0 16-17 38.99248069154942 40.0 38.0 41.0 35.5 41.0 18-19 39.112889625065904 40.5 38.5 41.0 36.0 41.0 20-21 39.20356677347782 41.0 39.0 41.0 36.0 41.0 22-23 39.15890483358589 41.0 39.0 41.0 35.5 41.0 24-25 39.08734209514949 41.0 39.0 41.0 35.0 41.0 26-27 38.894388935108935 40.0 38.0 41.0 35.0 41.0 28-29 38.72255297245752 40.0 38.0 41.0 35.0 41.0 30-31 38.482217228924654 40.0 38.0 41.0 34.5 41.0 32-33 38.24456231775387 40.0 37.0 41.0 34.0 41.0 34-35 38.14123616716206 40.0 37.0 41.0 34.0 41.0 36-37 37.98672467613401 40.0 37.0 41.0 33.5 41.0 38-39 37.809521092746394 40.0 37.0 41.0 33.0 41.0 40-41 37.5958623270954 40.0 37.0 41.0 33.0 41.0 42-43 37.385863060625304 40.0 36.5 41.0 33.0 41.0 44-45 37.05584513616384 40.0 36.0 41.0 32.0 41.0 46-47 36.5695568162796 40.0 36.0 41.0 30.5 41.0 48-49 36.087009498898766 40.0 35.0 41.0 29.5 41.0 50-51 35.77013649298726 39.0 35.0 41.0 28.0 41.0 52-53 35.482920978767126 39.0 35.0 41.0 27.0 41.0 54-55 35.246624038339164 39.0 35.0 41.0 27.0 41.0 56-57 34.99874108714147 38.5 35.0 40.0 26.0 41.0 58-59 34.7217545533712 38.0 35.0 40.0 26.0 41.0 60-61 34.33817797441674 38.0 34.0 40.0 24.5 41.0 62-63 33.93334125378442 37.0 34.0 40.0 23.0 41.0 64-65 33.5019589010092 36.5 33.5 39.5 22.0 41.0 66-67 33.13575883041479 36.0 33.0 39.0 21.5 40.5 68-69 32.68759557048251 35.5 33.0 39.0 20.5 40.0 70-71 32.244900556542305 35.0 32.5 38.0 18.0 40.0 72-73 31.775038604362184 35.0 32.0 37.0 15.0 39.5 74-75 31.306568164676836 35.0 31.0 37.0 10.0 39.0 76-77 29.338004309644916 33.0 28.5 35.0 6.5 37.0 78-79 30.281320943156448 34.0 30.5 36.0 6.5 38.0 80-81 30.334881791969664 34.0 31.0 35.5 2.0 37.0 82-83 30.06663304132156 34.0 31.0 35.0 2.0 37.0 84-85 29.713401089762115 34.0 30.5 35.0 2.0 36.5 86-87 29.178842427118788 34.0 30.0 35.0 2.0 36.0 88-89 28.62770880994491 34.0 29.0 35.0 2.0 35.5 90-91 28.172747545966306 34.0 29.0 35.0 2.0 35.0 92-93 27.925627591257076 34.0 29.0 35.0 2.0 35.0 94-95 27.632611234794147 34.0 27.5 35.0 2.0 35.0 96-97 27.082113030061564 33.5 27.0 35.0 2.0 35.0 98-99 26.365930169207168 33.0 25.0 35.0 2.0 35.0 100-101 22.924595633804316 29.0 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13809129465528258 1101 2 0.15036552061165764 1101 3 0.1968058902373926 1101 4 0.184549932463689 1101 5 0.15866905857708957 1101 6 0.168705034238819 1101 7 0.15419158245705233 1101 8 0.16780133646408757 1101 9 0.27037489505660517 1101 10-11 0.18803131761524128 1101 12-13 0.2280621163135379 1101 14-15 0.27116406862353415 1101 16-17 0.3254194744997747 1101 18-19 0.2532607854864253 1101 20-21 0.3631740227415037 1101 22-23 0.40033882838426393 1101 24-25 0.1416588703563022 1101 26-27 0.27048340822639005 1101 28-29 0.0927000945753349 1101 30-31 0.1698109180660552 1101 32-33 0.2387205283557563 1101 34-35 0.3876728220186223 1101 36-37 0.43466079968367666 1101 38-39 0.4143255293264758 1101 40-41 0.5421994791117655 1101 42-43 0.5421540025276457 1101 44-45 0.5174368103090785 1101 46-47 0.5071652067298373 1101 48-49 0.48032250953324507 1101 50-51 0.526441332741733 1101 52-53 0.6546842076093498 1101 54-55 0.6582576542384473 1101 56-57 0.5062184140952013 1101 58-59 0.5904222115543192 1101 60-61 0.5750874817907388 1101 62-63 0.5882472993863033 1101 64-65 0.3465766199507314 1101 66-67 0.4377013174335005 1101 68-69 0.26147604275887915 1101 70-71 0.33038230007263536 1101 72-73 0.3851563581406765 1101 74-75 0.3390068550254135 1101 76-77 0.24994108754390254 1101 78-79 0.3271157930185602 1101 80-81 0.4752198573348103 1101 82-83 0.5072351387335665 1101 84-85 0.32086618655445776 1101 86-87 0.5214275644328445 1101 88-89 0.6821131426852958 1101 90-91 0.5534002305070977 1101 92-93 0.48384982765823636 1101 94-95 0.28970094657342216 1101 96-97 0.3801541413322411 1101 98-99 0.3012327571781057 1101 100-101 -0.32319302065412003 1102 1 0.28568274246619296 1102 2 0.17330658832229062 1102 3 0.21883857591309663 1102 4 0.13716829678651976 1102 5 0.19913520057944112 1102 6 0.18929234743768575 1102 7 0.18218207526100372 1102 8 0.11823904798445994 1102 9 0.25764950778842177 1102 10-11 0.24573903296743538 1102 12-13 0.18010195650261096 1102 14-15 0.28604009725231094 1102 16-17 0.247372040344068 1102 18-19 0.2804902738717274 1102 20-21 0.3132976859595331 1102 22-23 0.244041114881 1102 24-25 0.2268586742100993 1102 26-27 0.25884995511525233 1102 28-29 0.33578259120574927 1102 30-31 0.18673442372401894 1102 32-33 0.13371344083221004 1102 34-35 0.14726965045232276 1102 36-37 0.23573049321861816 1102 38-39 0.0481773639098364 1102 40-41 0.10558096473172895 1102 42-43 0.028677525335005782 1102 44-45 0.06078753420112548 1102 46-47 -0.03910011049377715 1102 48-49 -0.1496900041661675 1102 50-51 -0.11416843544666477 1102 52-53 0.15728019733043652 1102 54-55 0.009773366032142405 1102 56-57 -0.16544569356013028 1102 58-59 -0.04438570181979884 1102 60-61 -0.1421701691172359 1102 62-63 -0.24475537216794407 1102 64-65 -0.3625179187707275 1102 66-67 -0.29588630927775483 1102 68-69 -0.306605449024147 1102 70-71 -0.3073718258307281 1102 72-73 -0.35365861864065806 1102 74-75 -0.3010495772036599 1102 76-77 -0.0714241115096641 1102 78-79 -0.1318148885895205 1102 80-81 -0.3797605682381544 1102 82-83 -0.3192261103319254 1102 84-85 -0.2824353400096875 1102 86-87 -0.1344199774424979 1102 88-89 -0.27893897271941626 1102 90-91 -0.16981924691867079 1102 92-93 -0.29232430301479795 1102 94-95 -0.39799463022237447 1102 96-97 -0.4209712877375935 1102 98-99 -0.31000063269464206 1102 100-101 -0.6296272594219339 1103 1 0.13844816239412694 1103 2 0.15104309768408086 1103 3 0.22538832354086225 1103 4 0.10964543781670955 1103 5 0.14631860502492344 1103 6 0.17790184601073378 1103 7 0.1311869761906408 1103 8 0.1968412521992775 1103 9 0.18559129525154816 1103 10-11 0.19384417428646827 1103 12-13 0.15222850786189923 1103 14-15 0.216468978306132 1103 16-17 0.26540595440041415 1103 18-19 0.32813541014645153 1103 20-21 0.2777049853411384 1103 22-23 0.28437096220180536 1103 24-25 0.16470906765542992 1103 26-27 0.18167915096719867 1103 28-29 0.2281416201456139 1103 30-31 0.2584862627499831 1103 32-33 0.3211687668125762 1103 34-35 0.3471408218299743 1103 36-37 0.32881492881634955 1103 38-39 0.37844931684082184 1103 40-41 0.3173075613013978 1103 42-43 0.2873480788376028 1103 44-45 0.2714048022598945 1103 46-47 0.21171644947850865 1103 48-49 0.062406019728349804 1103 50-51 0.059149754505959606 1103 52-53 0.22124632932168709 1103 54-55 0.20966154362516676 1103 56-57 0.1702242937663172 1103 58-59 0.20243230446569527 1103 60-61 0.34521985299606683 1103 62-63 0.4666151404150938 1103 64-65 0.29884280869760715 1103 66-67 0.35816933296589326 1103 68-69 0.0189065950392191 1103 70-71 0.13323409777421347 1103 72-73 0.087880815371566 1103 74-75 0.2334683306027685 1103 76-77 0.2960406960898645 1103 78-79 0.15800922004932616 1103 80-81 0.27671414273467576 1103 82-83 0.18606439245552409 1103 84-85 0.22464473990382672 1103 86-87 0.3726655967867565 1103 88-89 0.3037075711466777 1103 90-91 0.35592031414305936 1103 92-93 0.36046241392747547 1103 94-95 0.2097169820401028 1103 96-97 0.11135255665082511 1103 98-99 0.22349690218903007 1103 100-101 0.11848976008445433 1104 1 0.12292791018496985 1104 2 0.15519633336326422 1104 3 0.13379860049743542 1104 4 0.026924719392930285 1104 5 0.11147796584144487 1104 6 0.09161875418224241 1104 7 0.08767361197868695 1104 8 0.0875924364532068 1104 9 0.1305642106914675 1104 10-11 0.11003835348366664 1104 12-13 0.14849514862775237 1104 14-15 0.11078253596477339 1104 16-17 0.10411421210540084 1104 18-19 0.09339968948670929 1104 20-21 0.1309015097759243 1104 22-23 0.1281359487556415 1104 24-25 0.11900627542647868 1104 26-27 0.1574199040737838 1104 28-29 0.08046348629912359 1104 30-31 0.12351925593281265 1104 32-33 0.18490520176814584 1104 34-35 0.21991269435751093 1104 36-37 0.2387807721204851 1104 38-39 0.22489746564098567 1104 40-41 0.17850854957925577 1104 42-43 0.09017561787599249 1104 44-45 0.07693523267877822 1104 46-47 0.18644747561607034 1104 48-49 0.08246953742284546 1104 50-51 0.030697307789793626 1104 52-53 0.12918462468402225 1104 54-55 0.18728061147921693 1104 56-57 0.3909154002773363 1104 58-59 0.2975161077571258 1104 60-61 0.21526809325848006 1104 62-63 0.2114729186515305 1104 64-65 0.21617368954780858 1104 66-67 0.37461786623124027 1104 68-69 0.20054868628696454 1104 70-71 0.27020386464666757 1104 72-73 0.20497828538833573 1104 74-75 0.13624872206926142 1104 76-77 0.31111324871606527 1104 78-79 0.42416181630220606 1104 80-81 0.3287722455406623 1104 82-83 0.48058950713744153 1104 84-85 0.4034524122099903 1104 86-87 0.2351348142787053 1104 88-89 0.2375750075531009 1104 90-91 0.4107516249872063 1104 92-93 0.378308174591826 1104 94-95 0.16985907227610042 1104 96-97 0.29890074126695865 1104 98-99 0.23572845949558996 1104 100-101 -0.1253092212851712 1105 1 0.014102389184898811 1105 2 0.032917262422657245 1105 3 0.07164723544911311 1105 4 0.09892759869320855 1105 5 0.09711250813165151 1105 6 0.10295939441910207 1105 7 0.10599519722362771 1105 8 0.07592777792385874 1105 9 0.0917770868291683 1105 10-11 0.07226985000192343 1105 12-13 0.07006380461152162 1105 14-15 0.09770878590899201 1105 16-17 0.13569095884533056 1105 18-19 0.15988358655366852 1105 20-21 0.23771840839700076 1105 22-23 0.1949943100767868 1105 24-25 0.07839970154158493 1105 26-27 0.16366841252251163 1105 28-29 0.18094456615313703 1105 30-31 0.19107367451399426 1105 32-33 0.22940775608002184 1105 34-35 0.2483739179049067 1105 36-37 0.23294310722524614 1105 38-39 0.36810384977146526 1105 40-41 0.37339003067268806 1105 42-43 0.23564576453792796 1105 44-45 0.1342821049321472 1105 46-47 0.17803076092872772 1105 48-49 0.27246212574694084 1105 50-51 0.12199673755640106 1105 52-53 0.04838474575433338 1105 54-55 0.11026175439069164 1105 56-57 0.20740851343344957 1105 58-59 0.21807487439216544 1105 60-61 0.2763280793276337 1105 62-63 0.2288636788915639 1105 64-65 0.12387504169360142 1105 66-67 0.13035269897377333 1105 68-69 0.053565160773928255 1105 70-71 0.22481996117695502 1105 72-73 0.06767116843325738 1105 74-75 0.13958494634424667 1105 76-77 0.28304152619989864 1105 78-79 0.40416775340385414 1105 80-81 0.28814287396153304 1105 82-83 0.2951826507551516 1105 84-85 0.40185851384308435 1105 86-87 0.44270539496993067 1105 88-89 0.5114970646618069 1105 90-91 0.5079217035470904 1105 92-93 0.4667738994711961 1105 94-95 0.3629429309265184 1105 96-97 0.45667097155277503 1105 98-99 0.6391229876609295 1105 100-101 0.13881273764793534 1106 1 0.02185589344994554 1106 2 0.014620036970384831 1106 3 0.12789003655200304 1106 4 -0.033797384881623316 1106 5 0.03504598826063443 1106 6 0.04875084013632858 1106 7 0.0709305549074486 1106 8 0.013754047747454479 1106 9 0.13336094040737834 1106 10-11 0.13760078622279082 1106 12-13 0.12514859696715774 1106 14-15 0.11762035115196312 1106 16-17 0.021414394743182186 1106 18-19 0.022932730108273347 1106 20-21 0.10903717762269594 1106 22-23 0.08237469498926941 1106 24-25 0.16697396941889764 1106 26-27 0.12546443254610296 1106 28-29 0.18046830498884248 1106 30-31 0.2311820119020851 1106 32-33 0.18898809645899206 1106 34-35 0.12704827622373926 1106 36-37 0.20661129407906031 1106 38-39 0.10562828135066837 1106 40-41 0.16638411211403792 1106 42-43 0.184856256853152 1106 44-45 0.10740942954021193 1106 46-47 0.2802784662158473 1106 48-49 0.3143311862272995 1106 50-51 0.35102750141607686 1106 52-53 0.36892294148736227 1106 54-55 0.35528540938243225 1106 56-57 0.20770196728215495 1106 58-59 0.1831813562172684 1106 60-61 0.20306358050832074 1106 62-63 0.1876681190476006 1106 64-65 0.2935188560713229 1106 66-67 0.2842324749867018 1106 68-69 0.027709608792044094 1106 70-71 0.19547732528789652 1106 72-73 0.23260803805727903 1106 74-75 0.1875897350712208 1106 76-77 0.20003394725599932 1106 78-79 0.3200056019404336 1106 80-81 0.2998397931771919 1106 82-83 0.2884730724765099 1106 84-85 0.1481405154319937 1106 86-87 0.322110982551127 1106 88-89 0.3180223236846551 1106 90-91 0.5271425870149855 1106 92-93 0.5182637103687107 1106 94-95 0.4672090763861583 1106 96-97 0.39575971144682853 1106 98-99 0.4253483877065598 1106 100-101 0.11293931728182471 1107 1 0.06437191507652784 1107 2 0.060403009164772925 1107 3 0.06994863291139097 1107 4 0.017300905449040727 1107 5 0.02105315885181369 1107 6 0.08844089733405269 1107 7 0.07931320562554589 1107 8 0.08429486897324523 1107 9 0.18064561392107237 1107 10-11 0.11497871917201508 1107 12-13 0.10126423971097154 1107 14-15 0.18307026138910487 1107 16-17 0.14438624991185378 1107 18-19 0.16637390934173624 1107 20-21 0.1372811655986439 1107 22-23 0.11740112806376146 1107 24-25 0.08568574192224077 1107 26-27 0.21485154009452856 1107 28-29 0.18131831229511164 1107 30-31 0.23474838944884624 1107 32-33 0.23688401851143226 1107 34-35 0.30387588978419444 1107 36-37 0.33819216415253806 1107 38-39 0.3916378003887502 1107 40-41 0.36670010869051595 1107 42-43 0.3661380913554879 1107 44-45 0.5666019030288538 1107 46-47 0.387750571885519 1107 48-49 0.42341002098013547 1107 50-51 0.33861008846518104 1107 52-53 0.3655666194683249 1107 54-55 0.14808781106078328 1107 56-57 0.2151419775109531 1107 58-59 0.37043737867885085 1107 60-61 0.47075912785300744 1107 62-63 0.29765306289362314 1107 64-65 0.22180054966685248 1107 66-67 0.27014179362397783 1107 68-69 0.14509411739258127 1107 70-71 0.07582787012757564 1107 72-73 0.05648691560404018 1107 74-75 0.02612553062065004 1107 76-77 0.10561659693034997 1107 78-79 0.40125012223101564 1107 80-81 0.22533074904726647 1107 82-83 0.16459119413649503 1107 84-85 0.2591946549610533 1107 86-87 0.20698946347130232 1107 88-89 0.3970677843977519 1107 90-91 0.6241634516609444 1107 92-93 0.5788575543065875 1107 94-95 0.2885111858133449 1107 96-97 0.3962098304348025 1107 98-99 0.3946300499894626 1107 100-101 0.015463114877018569 1108 1 -0.022458250301475857 1108 2 0.0013327235118509861 1108 3 -0.0415338627930808 1108 4 2.328749507540806E-4 1108 5 0.03828227182432187 1108 6 0.052633605667388395 1108 7 0.01958516653620279 1108 8 0.020714274041971237 1108 9 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-0.29388663635083034 2316 100-101 0.11344812150392158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 10.0 4 17.0 5 45.0 6 103.0 7 233.0 8 477.0 9 873.0 10 1614.0 11 2623.0 12 4097.0 13 6189.0 14 7265.0 15 10158.0 16 14245.0 17 22360.0 18 22382.0 19 12568.0 20 9665.0 21 9641.0 22 9763.0 23 10196.0 24 11103.0 25 13012.0 26 15287.0 27 17940.0 28 21998.0 29 26227.0 30 31926.0 31 39695.0 32 49374.0 33 63908.0 34 90973.0 35 140720.0 36 216928.0 37 358361.0 38 320718.0 39 32330.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.21963940362811 18.766160966053004 14.923201201904021 20.090998428414863 2 15.333658928657634 22.05034773706025 39.806410800569516 22.809582533712597 3 20.856888315997157 27.240103145589384 29.924070250848416 21.97893828756504 4 12.581040947896179 17.170430343812363 37.27861660022056 32.9699121080709 5 12.772511060941289 39.31413073258321 32.62057632880196 15.292781877673544 6 29.086341485128465 36.4468438465305 17.892048222381188 16.57476644595985 7 27.248316172704286 35.30805434641545 19.916778838226563 17.5268506426537 8 26.2502139462216 33.125019200301935 19.492710781698367 21.132056071778095 9 27.49959718550219 17.908035412566683 20.62278569579073 33.969581706140396 10-11 24.988448471405185 27.04267702051439 25.81432790792883 22.154546600151594 12-13 28.16924102225229 24.7872293070901 26.71487692684826 20.328652743809354 14-15 24.335009713138742 25.372060078827364 27.844749027353842 22.448181180680056 16-17 20.597435923961104 29.067250491301095 28.563425881951687 21.771887702786113 18-19 20.357655499767557 27.58139128095991 31.91733805049634 20.143615168776194 20-21 22.39507544386396 25.637935909548865 31.16219976213569 20.804788884451487 22-23 21.31214706710294 25.887304729010857 31.01489818040698 21.785650023479228 24-25 21.641985680430338 25.997072137026496 31.66703238830861 20.693909794234557 26-27 21.801855911965497 25.28869706854686 32.38005882091095 20.529388198576687 28-29 20.604604185383696 26.184885898483223 32.664243301210576 20.546266614922505 30-31 20.764045239694013 26.58869659699142 31.95290071104741 20.694357452267155 32-33 20.675888245151963 26.59679115149775 31.606580954428985 21.1207396489213 34-35 21.469663025615272 25.90516838687065 31.526319716540986 21.098848870973104 36-37 21.89770607853442 25.546262860898477 32.11292505402737 20.443106006539725 38-39 21.87815401517596 25.566699807831412 32.17543057647128 20.379715600521354 40-41 21.042712898268196 26.205048748723204 32.25469977320196 20.497538579806633 42-43 20.716712924514905 26.8838512613454 31.104429247751142 21.295006566388555 44-45 21.034540266674355 26.970699927710644 30.497235386798195 21.4975244188168 46-47 21.515604158601136 27.034104855306325 29.561271655462434 21.88901933063011 48-49 21.914134538251435 26.82056665800428 29.613324234170324 21.65197456957396 50-51 21.978614135009128 27.547992179435376 29.659933336488976 20.81346034906652 52-53 21.62025673578784 27.940941364555417 29.409413645554146 21.0293882541026 54-55 20.591187484600574 27.41373656997656 29.854416257530435 22.14065968789243 56-57 21.59349026693592 28.128834182744246 28.722084328353066 21.555591221966775 58-59 21.841177586236622 27.20307594402986 29.076542791892575 21.879203677840945 60-61 21.40265801895074 26.981506979870485 30.044636810979185 21.57119819019959 62-63 22.02861259390506 26.92562581428272 29.641019660656493 21.404741931155726 64-65 21.47239821529389 27.41438617576904 29.793983873945805 21.319231734991266 66-67 21.05085130448085 27.476607622491795 29.540666748591192 21.931874324436162 68-69 20.806603501503474 28.080900479757638 29.343806662436705 21.76868935630218 70-71 21.21215540933019 28.06163408570328 28.618772164600397 22.10743834036613 72-73 21.119579793947064 27.443642019852955 28.735406986840978 22.70137119935901 74-75 20.93024130626002 28.353908742610628 28.439361841523684 22.27648810960567 76-77 21.437825461929748 27.228471761465308 28.96802807768864 22.365674698916298 78-79 21.055924725661576 27.306759355217714 29.48137294916236 22.15594296995835 80-81 20.96567242737566 28.38953548699773 28.79311636602911 21.851675719597495 82-83 20.801359988489125 28.068696597238834 28.643268536598594 22.486674877673448 84-85 20.770557488995482 27.56969004286448 28.57575451700818 23.083997951131863 86-87 21.089960017560767 27.57379303462666 28.724092298838062 22.61215464897452 88-89 21.299715003769002 26.970085624764927 28.782290261815696 22.94790910965037 90-91 20.695032189013407 27.1059280842966 29.258124826718724 22.94091489997127 92-93 20.52626695347477 27.80623776274634 28.879855952281687 22.787639331497207 94-95 20.231914569471236 27.784890161733937 28.593058299326596 23.390136969468227 96-97 19.833520059535044 27.323424619144376 28.64089448015614 24.202160841164442 98-99 20.43660092281929 27.34338827469658 28.176364503275387 24.04364629920875 100-101 20.729837540458146 26.680762689947574 28.92557268359966 23.663827085994622 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3485.0 1 2275.0 2 1654.0 3 2062.0 4 1465.0 5 1275.0 6 2355.0 7 5059.5 8 7735.0 9 8155.0 10 6345.0 11 4643.5 12 4430.0 13 4576.5 14 4851.0 15 5002.0 16 4928.5 17 4950.0 18 5080.0 19 5372.0 20 5747.5 21 6275.0 22 6956.5 23 7871.0 24 8899.0 25 10007.5 26 11577.0 27 13627.0 28 15494.5 29 17220.5 30 19183.5 31 21266.0 32 23564.0 33 25768.5 34 28022.0 35 29666.0 36 31427.0 37 33859.0 38 35435.0 39 36216.0 40 37078.5 41 39323.5 42 42247.5 43 44422.5 44 46722.5 45 49370.0 46 51962.0 47 61828.0 48 99810.5 49 100185.5 50 67181.5 51 57785.0 52 50045.5 53 47450.0 54 46124.0 55 42664.0 56 37833.5 57 33141.5 58 29834.5 59 26830.5 60 23269.5 61 19812.5 62 16469.5 63 12599.0 64 9124.0 65 7045.5 66 5356.5 67 4407.0 68 3423.5 69 2364.0 70 1745.5 71 1303.0 72 1058.0 73 918.0 74 749.5 75 597.0 76 385.5 77 202.0 78 136.5 79 82.5 80 44.0 81 26.5 82 14.5 83 5.0 84 2.0 85 2.5 86 2.0 87 0.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3478937974090721 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014106344281319375 16-17 0.0017241087454945903 18-19 3.134743173626528E-5 20-21 0.0 22-23 0.0 24-25 4.3886404430771393E-4 26-27 0.001003117815560489 28-29 0.0 30-31 0.0035109123544617114 32-33 0.0 34-35 0.009905788428659829 36-37 0.029153111514726708 38-39 0.020908736968088943 40-41 0.013542090510066601 42-43 0.010187915314286216 44-45 0.0030093534466814668 46-47 0.0012225498377143459 48-49 5.329063395165097E-4 50-51 9.404229520879584E-5 52-53 1.2538972694506111E-4 54-55 0.0 56-57 0.0 58-59 0.004357293011340874 60-61 0.009717703838242237 62-63 0.004075166125714486 64-65 0.0019121933359121822 66-67 0.003949776398769425 68-69 0.0034482174909891807 70-71 3.134743173626528E-5 72-73 0.0 74-75 0.0 76-77 0.2158584149359227 78-79 5.015589077802445E-4 80-81 0.0034795649227254456 82-83 8.463806568791626E-4 84-85 0.0 86-87 0.10673800506198328 88-89 0.40184272742718463 90-91 0.389241059869206 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0014733292916044682 100-101 9.090755203516931E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1595027.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.417142720203955 #Duplication Level Percentage of deduplicated Percentage of total 1 75.15962255400102 37.89333417101729 2 14.03890772444881 14.156032287586187 3 4.815547533480827 7.28358441814287 4 2.0532535356024937 4.140767061809371 5 1.1190877950105307 2.8210604538742134 6 0.6665680960981614 2.0163875300229375 7 0.42611862574008674 1.5038578498772588 8 0.31468479099963576 1.269240641576495 9 0.19800641214874617 0.8984625786736975 >10 1.0747698827935208 9.799775533635637 >50 0.07539790283636516 2.6069600600230287 >100 0.04832125330712842 4.603541846998707 >500 0.004607872573231336 1.6459886185293788 >1k 0.0042342612835098775 4.636520448090156 >5k 3.7361128972145975E-4 1.5097385593186692 >10k+ 4.981483862952796E-4 3.2147479408241155 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16284 1.0209231567866877 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13190 0.8269452492026781 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11678 0.7321506156322118 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 10048 0.629957988171987 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9435 0.5915260368633258 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8463 0.530586629568026 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6147 0.3853853257656453 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4131 0.25899248100502376 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3832 0.24024671682673712 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3726 0.23360106129864888 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3527 0.22112478346761527 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3355 0.21034126695034006 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3181 0.1994323607061197 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2918 0.18294361161284417 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2811 0.1762352612212834 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2794 0.1751694485422504 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2712 0.17002846973750288 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2646 0.16589060874831588 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2371 0.14864952129336995 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2353 0.1475210137508644 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2082 0.13053070574980863 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1967 0.1233207964504676 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1913 0.11993527382295095 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1906 0.11949640977864326 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1896 0.11886946114391794 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1771 0.11103260320985162 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1757 0.1101548751212362 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1726 0.10821133435358775 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1694 0.10620509872246676 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1691 0.10601701413204918 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.269486347253056E-5 0.0 12-13 0.0 0.0 0.0 1.2538972694506111E-4 0.0 14-15 0.0 0.0 0.0 1.8808459041759167E-4 0.0 16-17 0.0 0.0 0.0 2.1943202215385696E-4 0.0 18-19 0.0 0.0 0.0 2.5077945389012223E-4 0.0 20-21 0.0 0.0 0.0 4.3886404430771393E-4 0.0 22-23 0.0 0.0 0.0 9.404229520879584E-4 0.0 24-25 0.0 0.0 0.0 0.0017554561772308557 0.0 26-27 0.0 0.0 0.0 0.0024450996754286918 0.0 28-29 0.0 0.0 0.0 0.006677002959824504 0.0 30-31 0.0 0.0 0.0 0.016645486251956863 0.0 32-33 0.0 0.0 0.0 0.03194303293925432 0.0 34-35 0.0 0.0 0.0 0.057585232099519316 0.0 36-37 0.0 0.0 0.0 0.09739647040457622 0.0 38-39 0.0 0.0 0.0 0.15444879616457902 0.0 40-41 6.269486347253056E-5 0.0 0.0 0.2230056293717912 0.0 42-43 6.269486347253056E-5 0.0 0.0 0.28861580399579445 0.0 44-45 6.269486347253056E-5 0.0 0.0 0.36698438333645766 3.134743173626528E-5 46-47 6.269486347253056E-5 0.0 0.0 0.4624686604051217 6.269486347253056E-5 48-49 6.269486347253056E-5 0.0 0.0 0.5685483694006435 6.269486347253056E-5 50-51 6.269486347253056E-5 0.0 0.0 0.6900196673786714 6.269486347253056E-5 52-53 6.269486347253056E-5 0.0 0.0 0.8050333944190287 6.269486347253056E-5 54-55 6.269486347253056E-5 0.0 0.0 0.9283855383012325 6.269486347253056E-5 56-57 6.269486347253056E-5 0.0 0.0 1.0804832770855917 6.269486347253056E-5 58-59 6.269486347253056E-5 0.0 0.0 1.26414787962837 6.269486347253056E-5 60-61 6.269486347253056E-5 0.0 0.0 1.4839560709630621 1.2538972694506111E-4 62-63 6.269486347253056E-5 0.0 0.0 1.7058958876558203 1.2538972694506111E-4 64-65 6.269486347253056E-5 0.0 0.0 1.9142936138385118 1.2538972694506111E-4 66-67 6.269486347253056E-5 0.0 0.0 2.1537879923035783 1.2538972694506111E-4 68-69 6.269486347253056E-5 0.0 0.0 2.436855300882054 1.2538972694506111E-4 70-71 6.269486347253056E-5 0.0 0.0 2.7547496061195202 1.2538972694506111E-4 72-73 6.269486347253056E-5 0.0 0.0 3.0896028719263056 1.2538972694506111E-4 74-75 6.269486347253056E-5 6.269486347253056E-5 0.0 3.432637817416257 1.2538972694506111E-4 76-77 6.269486347253056E-5 6.269486347253056E-5 0.0 3.7873967023755712 1.2538972694506111E-4 78-79 6.269486347253056E-5 6.269486347253056E-5 0.0 4.188079574828514 1.2538972694506111E-4 80-81 6.269486347253056E-5 6.269486347253056E-5 0.0 4.641018615985811 1.2538972694506111E-4 82-83 6.269486347253056E-5 6.269486347253056E-5 0.0 5.111292786893262 1.2538972694506111E-4 84-85 6.269486347253056E-5 6.269486347253056E-5 0.0 5.622161881899178 1.2538972694506111E-4 86-87 6.269486347253056E-5 6.269486347253056E-5 0.0 6.155099568847424 1.2538972694506111E-4 88-89 6.269486347253056E-5 6.269486347253056E-5 0.0 6.694181352415978 1.2538972694506111E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 65 1.3362127E-4 36.502132 2 TACCTGG 1550 0.0 28.165518 2 TAGACCG 90 9.001196E-4 26.362654 5 GAGTACT 11630 0.0 22.788654 12-13 GTAAGCG 115 3.6725396E-9 22.695517 94-95 GGGTACG 105 0.0021666032 22.67261 1 TATAGGG 315 0.0 22.596561 2 AGTACTT 11785 0.0 21.784266 12-13 GTACTTT 12425 0.0 21.21597 14-15 TCGCGCT 150 1.6916601E-10 20.56577 60-61 GTATAGG 400 0.0 20.235304 1 GTACATG 15800 0.0 20.129833 1 CATGGGG 4045 0.0 20.060379 4 TACATGG 15910 0.0 19.65517 2 AGGACCG 290 2.4701876E-9 19.63563 5 TTAACGC 145 6.1887887E-4 19.63563 3 TAACGCA 195 1.0419659E-5 19.467806 4 TACTTTT 13325 0.0 19.266607 14-15 ACTTTTT 13890 0.0 19.029236 16-17 GTACCGT 125 0.0060668993 18.981112 6 >>END_MODULE