##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939156_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1568755 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20493257392008 33.0 31.0 34.0 31.0 34.0 2 32.27473059846821 34.0 31.0 34.0 31.0 34.0 3 32.31481397668852 34.0 31.0 34.0 30.0 34.0 4 35.76162625776492 37.0 35.0 37.0 35.0 37.0 5 35.823336977412026 37.0 35.0 37.0 35.0 37.0 6 35.93285248493232 37.0 37.0 37.0 35.0 37.0 7 35.88280579185405 37.0 37.0 37.0 35.0 37.0 8 35.88418331734401 37.0 37.0 37.0 35.0 37.0 9 37.556296553636486 39.0 38.0 39.0 35.0 39.0 10-11 37.46584616463374 39.0 38.0 39.0 35.0 39.0 12-13 37.370449815299395 39.0 37.5 39.0 35.0 39.0 14-15 38.58884051365573 40.0 38.0 41.0 34.0 41.0 16-17 38.532897425028125 40.0 38.0 41.0 34.0 41.0 18-19 38.52760246182483 40.0 38.0 41.0 34.0 41.0 20-21 38.54301117765361 40.0 38.0 41.0 34.0 41.0 22-23 38.567691895802724 40.0 38.0 41.0 34.0 41.0 24-25 38.50068238826331 40.0 38.0 41.0 34.0 41.0 26-27 38.34555778308276 40.0 38.0 41.0 34.0 41.0 28-29 38.19622821919293 40.0 38.0 41.0 34.0 41.0 30-31 37.9457786588728 40.0 37.5 41.0 33.5 41.0 32-33 37.676218402491145 40.0 37.0 41.0 33.0 41.0 34-35 37.34160369209979 40.0 36.5 41.0 32.5 41.0 36-37 37.03988385694389 40.0 36.0 41.0 31.5 41.0 38-39 36.81877507960134 40.0 35.5 41.0 31.0 41.0 40-41 36.59664192305363 40.0 35.0 41.0 30.5 41.0 42-43 36.35671631325478 39.0 35.0 41.0 30.0 41.0 44-45 35.981034960844745 39.0 35.0 41.0 29.5 41.0 46-47 35.6040889112704 39.0 35.0 41.0 26.5 41.0 48-49 35.32960341162259 39.0 35.0 41.0 24.5 41.0 50-51 34.13755143409901 38.0 34.0 40.0 23.0 40.5 52-53 34.24398838569439 38.5 34.5 39.5 23.0 40.5 54-55 34.764044417388305 39.0 35.0 40.5 23.0 41.0 56-57 34.684505547392675 39.0 35.0 41.0 23.0 41.0 58-59 34.49135429050425 39.0 35.0 41.0 22.0 41.0 60-61 34.176047247658175 38.0 34.5 40.5 21.0 41.0 62-63 33.7837893743765 37.5 34.0 40.0 19.5 41.0 64-65 33.38803382299977 37.0 34.0 40.0 17.5 41.0 66-67 32.99021357700852 36.5 33.5 39.5 13.0 41.0 68-69 32.58374252193619 36.0 33.0 39.0 9.0 41.0 70-71 32.115653495925116 35.5 33.0 39.0 6.5 40.5 72-73 31.65566962336375 35.0 32.0 37.5 2.0 40.0 74-75 31.231988105217194 35.0 32.0 37.0 2.0 39.5 76-77 30.780574404543728 35.0 31.0 36.5 2.0 39.0 78-79 30.351334657100693 35.0 31.0 36.0 2.0 39.0 80-81 29.930051537684342 35.0 31.0 36.0 2.0 37.5 82-83 29.55930244047031 34.5 30.0 35.0 2.0 37.0 84-85 29.19407874397213 34.0 30.0 35.0 2.0 36.5 86-87 28.78771446146785 34.0 29.0 35.0 2.0 36.0 88-89 28.346085271441368 34.0 29.0 35.0 2.0 36.0 90-91 27.91163183543638 34.0 28.5 35.0 2.0 35.5 92-93 27.511864504017517 34.0 27.0 35.0 2.0 35.0 94-95 27.044472527577604 34.0 26.0 35.0 2.0 35.0 96-97 26.443264563300197 33.5 25.0 35.0 2.0 35.0 98-99 25.761282673202636 33.0 22.0 35.0 2.0 35.0 100-101 23.040883375670518 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19864226845191268 1101 2 0.361871607896056 1101 3 0.21954729084593794 1101 4 0.27292105095500574 1101 5 0.1604314157917699 1101 6 0.22156245473459535 1101 7 0.30233491711781824 1101 8 0.3019066767527008 1101 9 0.2955971362243801 1101 10-11 0.40164965944757114 1101 12-13 0.3679250610643052 1101 14-15 0.48104542183576626 1101 16-17 0.5030978747730757 1101 18-19 0.5295371555587707 1101 20-21 0.5325879786104224 1101 22-23 0.6182607929278703 1101 24-25 0.5811815678655421 1101 26-27 0.6494414461500355 1101 28-29 0.6459864491040221 1101 30-31 0.5708955769913047 1101 32-33 0.5309328738106203 1101 34-35 0.6280490239500267 1101 36-37 0.7149266733791393 1101 38-39 0.6940579013630526 1101 40-41 0.6161168681330693 1101 42-43 0.42684071233715315 1101 44-45 0.3058326537935585 1101 46-47 0.649897720659169 1101 48-49 0.547510162894163 1101 50-51 0.5873527152550437 1101 52-53 0.45331880910029554 1101 54-55 0.5985831801349377 1101 56-57 0.5054436990221447 1101 58-59 0.4834905862308645 1101 60-61 0.4007758213118038 1101 62-63 0.33474627115754174 1101 64-65 0.2863152669302238 1101 66-67 0.352748923889628 1101 68-69 0.3762967061482527 1101 70-71 0.3633181496724234 1101 72-73 0.26426791806083116 1101 74-75 0.3757565738502713 1101 76-77 0.4194616177216801 1101 78-79 0.20844750409715118 1101 80-81 -0.028036687710486063 1101 82-83 -0.09738666345815616 1101 84-85 -0.16145508959019494 1101 86-87 -0.19067730687993745 1101 88-89 -0.280829813506255 1101 90-91 -0.2577921556517815 1101 92-93 -0.27797669126092117 1101 94-95 -0.48283689115664785 1101 96-97 -0.5542322569271612 1101 98-99 -0.7015522916370465 1101 100-101 -0.9292723001897194 1102 1 0.39940461049517495 1102 2 0.38006786690940686 1102 3 0.2794358733404039 1102 4 0.28147592530354615 1102 5 0.3129611776965291 1102 6 0.27160774458967296 1102 7 0.31118428707569734 1102 8 0.3435052970853931 1102 9 0.41487120454736015 1102 10-11 0.48427854589721164 1102 12-13 0.4432785673468729 1102 14-15 0.4731626444744492 1102 16-17 0.5248252923143042 1102 18-19 0.6072449534455444 1102 20-21 0.5610687159203351 1102 22-23 0.5782439709817027 1102 24-25 0.6613596707783671 1102 26-27 0.7060515339632758 1102 28-29 0.6958939730206382 1102 30-31 0.6418054345625208 1102 32-33 0.46093662193656115 1102 34-35 0.5490907352372361 1102 36-37 0.7007351084473186 1102 38-39 0.5116028119434759 1102 40-41 0.6320692713957214 1102 42-43 0.4875079233744941 1102 44-45 0.39881016057588425 1102 46-47 0.6674826781804057 1102 48-49 0.475280652649289 1102 50-51 0.33204231242074655 1102 52-53 0.2801771433260356 1102 54-55 0.2894386452595157 1102 56-57 0.2582804502893694 1102 58-59 0.3131187409035263 1102 60-61 0.24341867399260053 1102 62-63 0.22508179535972772 1102 64-65 0.10822462475131545 1102 66-67 0.044775120612705166 1102 68-69 -0.09255369008219816 1102 70-71 -0.0726396526764006 1102 72-73 0.03372749630742078 1102 74-75 0.03607150567007622 1102 76-77 -0.013118409229129213 1102 78-79 -0.15084160135013036 1102 80-81 -0.041654878615037916 1102 82-83 -0.1481810207437988 1102 84-85 -0.2522500938737693 1102 86-87 -0.3042706218296054 1102 88-89 -0.33392937979453663 1102 90-91 -0.22559006473295895 1102 92-93 -0.44361095002439654 1102 94-95 -0.5928811993596881 1102 96-97 -0.5930002613178225 1102 98-99 -0.8686791076933069 1102 100-101 -1.1705618116839709 1103 1 0.2560449433298828 1103 2 0.28486093596949047 1103 3 0.25122049687568193 1103 4 0.2690686390906336 1103 5 0.23447778826746202 1103 6 0.29671448231093933 1103 7 0.29213900535043535 1103 8 0.3276154005508616 1103 9 0.24652556131624692 1103 10-11 0.3054013176498884 1103 12-13 0.38077661957823494 1103 14-15 0.4546218348259785 1103 16-17 0.37614237346613777 1103 18-19 0.49152801876591923 1103 20-21 0.4089031867505568 1103 22-23 0.46142849479122106 1103 24-25 0.48217469508652044 1103 26-27 0.39168167607246573 1103 28-29 0.3967887779321302 1103 30-31 0.5303502176590058 1103 32-33 0.42339922477874126 1103 34-35 0.39129810247732166 1103 36-37 0.26584511841989666 1103 38-39 0.4395957532299377 1103 40-41 0.3769485876510217 1103 42-43 0.3755774509237284 1103 44-45 0.3282249729668081 1103 46-47 0.44121429124448497 1103 48-49 0.5864982417791822 1103 50-51 0.5040010664721066 1103 52-53 0.4908575378660558 1103 54-55 0.4872540279570998 1103 56-57 0.5426118202719223 1103 58-59 0.5005856288367099 1103 60-61 0.452797959083604 1103 62-63 0.4502535885534513 1103 64-65 0.4578140351228015 1103 66-67 0.5605462032604223 1103 68-69 0.60925072278242 1103 70-71 0.43951247584516295 1103 72-73 0.4557758477160476 1103 74-75 0.3712980701178452 1103 76-77 0.3329124278439224 1103 78-79 0.2987501463437141 1103 80-81 0.36067621088882973 1103 82-83 0.2949616649954159 1103 84-85 0.25390875055405715 1103 86-87 0.2163153448869224 1103 88-89 0.2013992477297677 1103 90-91 0.2549108234495385 1103 92-93 -0.02688000699273374 1103 94-95 -0.17339026817045777 1103 96-97 -0.32403873364392766 1103 98-99 -0.35614132098175943 1103 100-101 -0.2134068783755687 1104 1 0.22164545167405691 1104 2 0.2969903665682949 1104 3 0.29034928240030666 1104 4 0.27069043273976234 1104 5 0.40040276249663975 1104 6 0.3076780645514674 1104 7 0.39111717355406483 1104 8 0.3126752895809588 1104 9 0.42603191883307545 1104 10-11 0.4460837461291618 1104 12-13 0.3980867352415416 1104 14-15 0.5872161903197224 1104 16-17 0.5102971762684589 1104 18-19 0.600757455151097 1104 20-21 0.5049549469886969 1104 22-23 0.5651431898382953 1104 24-25 0.5860584274300251 1104 26-27 0.5580521479624494 1104 28-29 0.6428843110613016 1104 30-31 0.6967391738175337 1104 32-33 0.6398406333978741 1104 34-35 0.688762757341209 1104 36-37 0.6042135843649064 1104 38-39 0.5776812759221883 1104 40-41 0.8357645657742196 1104 42-43 0.9676915261852415 1104 44-45 1.0422674449939535 1104 46-47 1.0002787622300744 1104 48-49 1.0017551034611358 1104 50-51 0.8696231130074565 1104 52-53 0.9055001324787284 1104 54-55 1.0045003862883064 1104 56-57 1.0289666796521786 1104 58-59 0.9888813364145079 1104 60-61 1.2086371127291287 1104 62-63 1.1096659388308723 1104 64-65 1.0090275139585785 1104 66-67 0.9599952649025099 1104 68-69 0.883935257932535 1104 70-71 1.0150737546779425 1104 72-73 0.9612254922823169 1104 74-75 0.8513814047013248 1104 76-77 0.7383707519797653 1104 78-79 0.7754358580577048 1104 80-81 0.7390280249188699 1104 82-83 0.8166110296723552 1104 84-85 0.8089771369899239 1104 86-87 0.9069227111626184 1104 88-89 0.8113134057150795 1104 90-91 0.8746101245834588 1104 92-93 0.8114236300683864 1104 94-95 0.6872367396497268 1104 96-97 0.710849385632649 1104 98-99 0.5399703925795833 1104 100-101 0.6394832575614906 1105 1 0.025856283821852344 1105 2 0.10605021789175595 1105 3 0.08395739348692643 1105 4 0.16885000955263507 1105 5 0.16812372348407933 1105 6 0.23855850216542507 1105 7 0.13015177791818644 1105 8 0.13821579492088887 1105 9 0.19596198876314475 1105 10-11 0.2129160542222195 1105 12-13 0.16991182991763765 1105 14-15 0.1621595850338906 1105 16-17 0.15892712797864306 1105 18-19 0.11577288384847861 1105 20-21 0.16284662551074547 1105 22-23 0.22776216112488612 1105 24-25 0.24278293501413373 1105 26-27 0.25084424125597593 1105 28-29 0.28607514601430495 1105 30-31 0.3721594555415422 1105 32-33 0.35700305550299305 1105 34-35 0.264306769203273 1105 36-37 0.2561817451439623 1105 38-39 0.34796800924617344 1105 40-41 0.23354644006038683 1105 42-43 0.2504439456855181 1105 44-45 0.3090025723307974 1105 46-47 0.12835097654870964 1105 48-49 0.3021017482203874 1105 50-51 0.1446135953045271 1105 52-53 0.22328871925011384 1105 54-55 0.16109615441703085 1105 56-57 0.25173803437195374 1105 58-59 0.15404604276832856 1105 60-61 0.16456263419906492 1105 62-63 0.03849806658850241 1105 64-65 0.08205183920353676 1105 66-67 0.10967116207124405 1105 68-69 0.12539086536235544 1105 70-71 -0.00983006642932338 1105 72-73 0.09921565398308019 1105 74-75 0.11718830553687454 1105 76-77 0.23595075077753336 1105 78-79 0.12348823547970866 1105 80-81 0.31440572744557116 1105 82-83 0.24158598308570234 1105 84-85 0.32975227875360247 1105 86-87 0.16440913591265272 1105 88-89 0.23293812769796673 1105 90-91 0.1904485841181902 1105 92-93 0.17301669316574575 1105 94-95 0.2576276667741766 1105 96-97 0.03674655436649488 1105 98-99 0.015624609422911107 1105 100-101 0.014843928409266738 1106 1 0.15054548351105268 1106 2 0.07064326373480156 1106 3 0.19079500032453467 1106 4 0.11019616339878979 1106 5 0.11112586023621418 1106 6 0.155288300145223 1106 7 0.12710690612331632 1106 8 0.04970569391078783 1106 9 0.05658747438862832 1106 10-11 0.14620810938927775 1106 12-13 0.07460297210878508 1106 14-15 0.13653070003000778 1106 16-17 0.16354057009208134 1106 18-19 0.1890901915407852 1106 20-21 0.22498240639652778 1106 22-23 0.23027770114042312 1106 24-25 0.203299154905352 1106 26-27 0.15401780380453545 1106 28-29 0.2764743301634951 1106 30-31 0.2984026567847451 1106 32-33 0.32274217749211687 1106 34-35 0.3330689098404065 1106 36-37 0.2923025687647822 1106 38-39 0.33771888337204814 1106 40-41 0.40178653330048775 1106 42-43 0.46141665353322026 1106 44-45 0.3912456764489036 1106 46-47 0.30776045595818147 1106 48-49 0.3836139859826275 1106 50-51 0.5059262409921743 1106 52-53 0.4868983734847703 1106 54-55 0.3547660262118981 1106 56-57 0.3137864026703241 1106 58-59 0.33377846312575343 1106 60-61 0.4505863327227573 1106 62-63 0.3270907239311569 1106 64-65 0.14051629141798827 1106 66-67 0.12148643013650684 1106 68-69 0.24440067499716633 1106 70-71 0.16225618065693226 1106 72-73 0.15984356773599728 1106 74-75 0.22798618820975847 1106 76-77 0.36508099553277873 1106 78-79 0.27485284309431535 1106 80-81 0.2788312324960778 1106 82-83 0.24876838401810275 1106 84-85 0.17805744580877203 1106 86-87 0.35210338610690073 1106 88-89 0.5023801440149818 1106 90-91 0.4073216416162495 1106 92-93 0.20001504203909448 1106 94-95 0.35776364191015375 1106 96-97 0.46763961963455714 1106 98-99 0.48208974151304673 1106 100-101 0.14905624387158056 1107 1 -0.05446943073205546 1107 2 -0.12141024111232923 1107 3 -0.06229970511916605 1107 4 -0.06052531311127751 1107 5 0.04029718760384782 1107 6 0.050333975014723364 1107 7 -0.06065875383194452 1107 8 -0.021332710265198784 1107 9 -0.026988215395114423 1107 10-11 0.07945521975467074 1107 12-13 0.04543630544211652 1107 14-15 -0.008936768277230556 1107 16-17 -0.04989157010180634 1107 18-19 -0.08727306349277342 1107 20-21 -0.03859421552740372 1107 22-23 0.020536836114331436 1107 24-25 0.028595109416912123 1107 26-27 -0.03612248329464052 1107 28-29 0.018993905107571152 1107 30-31 -0.06157124328982633 1107 32-33 0.08585272931910737 1107 34-35 -0.12253558307531875 1107 36-37 0.015634037817733315 1107 38-39 0.008671064580099141 1107 40-41 0.16045543710361443 1107 42-43 0.14091656031919797 1107 44-45 0.18120419621027395 1107 46-47 0.28982607862783283 1107 48-49 0.35764922088195306 1107 50-51 0.2018224937885904 1107 52-53 0.1646225532014043 1107 54-55 0.0163362163685008 1107 56-57 -0.05322981656905057 1107 58-59 0.01034678900195729 1107 60-61 -0.17956840254942108 1107 62-63 -0.10717941029702871 1107 64-65 0.16737591110478434 1107 66-67 0.051002649375881504 1107 68-69 -0.07645829219150357 1107 70-71 -0.13746417728490457 1107 72-73 -0.22472718170472206 1107 74-75 -0.41214477748748024 1107 76-77 -0.2427839373531313 1107 78-79 -0.16927001298323674 1107 80-81 -0.18483161239579715 1107 82-83 -0.1540140544606423 1107 84-85 -0.14853185322180096 1107 86-87 -0.24419601732337526 1107 88-89 -0.029492059564436346 1107 90-91 -0.05365663951723221 1107 92-93 -0.13955663954181574 1107 94-95 -0.011382517672249293 1107 96-97 -0.13143057052203844 1107 98-99 -0.23353059778598606 1107 100-101 -0.15690479266831403 1108 1 -0.023793140827571335 1108 2 0.08244881929035586 1108 3 0.04357277810230897 1108 4 0.12613531683793866 1108 5 0.14323564859600424 1108 6 0.0840250726319951 1108 7 0.04076431353072252 1108 8 0.1489781806832724 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-1.542912188264424 2316 52-53 -1.3627244282738218 2316 54-55 -1.4374926668336698 2316 56-57 -1.416054724428001 2316 58-59 -1.190624514492157 2316 60-61 -1.141549958244461 2316 62-63 -1.2030801393781942 2316 64-65 -1.3106977373589928 2316 66-67 -1.329337910391068 2316 68-69 -1.4115370748029967 2316 70-71 -1.2254536514773626 2316 72-73 -1.5987820317843884 2316 74-75 -1.2654465655872045 2316 76-77 -1.1519079133401249 2316 78-79 -0.8313272128609199 2316 80-81 -0.9741488834111998 2316 82-83 -0.9990883306101921 2316 84-85 -1.009027885925839 2316 86-87 -0.9908322653719175 2316 88-89 -1.0976273499898106 2316 90-91 -1.2082484023485769 2316 92-93 -1.1838253856187784 2316 94-95 -1.2461732086094344 2316 96-97 -1.3888726705333063 2316 98-99 -1.6053627964565784 2316 100-101 -1.3103812061683833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4766.0 3 1084.0 4 1114.0 5 1163.0 6 1442.0 7 1709.0 8 2073.0 9 2750.0 10 3314.0 11 4447.0 12 6511.0 13 11054.0 14 9654.0 15 10854.0 16 14596.0 17 23526.0 18 19266.0 19 11213.0 20 10047.0 21 10105.0 22 10391.0 23 11026.0 24 12018.0 25 13579.0 26 15289.0 27 17729.0 28 20836.0 29 24716.0 30 29724.0 31 36285.0 32 45654.0 33 60612.0 34 85502.0 35 136038.0 36 207193.0 37 324144.0 38 310157.0 39 57141.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.879909387308466 18.36775570408813 14.422648053522922 20.329686855080485 2 15.039579520246694 21.55954286356106 40.58674947605956 22.814128140132688 3 20.909364164948375 27.292475955079986 29.618184985861184 22.179974894110455 4 12.335026417473955 16.777425601525692 37.55493800605834 33.33260997494201 5 12.825885762064937 39.14394949991383 32.69535912378011 15.334805614241127 6 29.0399252883975 35.99840013064324 18.3729175544198 16.58875702653946 7 27.256244449661622 35.19546122673962 20.118828278910243 17.429466044688517 8 26.098966646634995 32.83522103095467 19.79728462448278 21.268527697927553 9 27.671562513755816 17.963418625228787 20.7652985386461 33.5997203223693 10-11 24.947003657273946 26.841826251099636 25.981987303843358 22.22918278778306 12-13 28.193891898650836 24.698084583525784 26.691715173027998 20.416308344795382 14-15 24.46303840887322 25.18277219189796 27.893998029463635 22.460191369765184 16-17 20.682382751348268 28.969497935015177 28.503074192729365 21.845045120907187 18-19 20.497062032269795 27.611601304062116 31.738377323099893 20.1529593405682 20-21 22.476011840963608 25.64091767467973 30.966895064563875 20.916175419792783 22-23 21.441471389454534 25.940985037458198 30.701492170673976 21.916051402413288 24-25 21.64282325328664 26.066253175922245 31.41896839600412 20.871955174787 26-27 21.87275652295438 25.246499298360032 32.18801270928749 20.692731469398097 28-29 20.667164175771095 26.271798859132282 32.41837601670048 20.642660948396138 30-31 20.741370509793914 26.661902635104955 31.775569450647613 20.82115740445352 32-33 20.69070462003143 26.63770865939271 31.368087195588412 21.303499524987444 34-35 21.587457022309223 25.997845159107403 31.19607101316858 21.218626805414797 36-37 21.929725346148953 25.658358280067528 31.82836945923768 20.583546914545835 38-39 21.977850184571114 25.582392911751523 31.9213688279678 20.518388075709556 40-41 21.123444655501462 26.235444191752507 32.053219676407 20.587891476339035 42-43 20.686292568927986 27.02031519547149 30.87140864088571 21.421983594714813 44-45 21.04480079351959 27.082956885691917 30.28689475228784 21.58534756850066 46-47 21.515200833487647 26.989480806653038 29.412669294168982 22.082649065690337 48-49 21.885178513872095 26.901297531236807 29.485050099994936 21.728473854896162 50-51 21.94507427759286 27.635711555534627 29.529600342432726 20.88961382443979 52-53 21.577647942340423 27.937541009657775 29.323571326047283 21.16123972195452 54-55 20.627285644033016 27.40264337785951 29.836947380730027 22.13312359737745 56-57 21.58719564006586 28.14099708255171 28.66549080486534 21.606316472517086 58-59 21.783370135932092 27.240637755142888 29.032910970803982 21.943081138121034 60-61 21.428361084970586 26.952441311849025 30.073332181016216 21.54586542216418 62-63 22.06675708209717 26.848001496531264 29.657008615945617 21.42823280542595 64-65 21.470013764677837 27.40737679075383 29.834875131046395 21.287734313521938 66-67 21.04901569662275 27.45314805646747 29.53323495907214 21.96460128783764 68-69 20.7503336226225 28.147237502131212 29.322640316995972 21.779788558250317 70-71 21.29548355504879 28.024051728585796 28.53580631711992 22.144658399245493 72-73 21.17392754618363 27.407920324449343 28.74037483934701 22.677777290020018 74-75 20.925149516394892 28.304261057035408 28.550998356793674 22.219591069776026 76-77 21.566202724067615 27.220781467498124 28.921697872933443 22.29131793550082 78-79 21.21237647260794 27.34513875232129 29.403506906387904 22.038977868682863 80-81 21.09630119343102 28.411787673762422 28.695099018035513 21.796812114771043 82-83 20.992734813151852 27.97236288197171 28.596658315177574 22.43824398969887 84-85 20.9936164378647 27.561080772177814 28.527520302597758 22.917782487359727 86-87 21.35919375551977 27.53313624195279 28.755822487060872 22.35184751546657 88-89 21.63343449445974 26.940757468628888 28.65692986820434 22.76887816870703 90-91 21.00198821171491 27.095560728573055 29.236965678114775 22.665485381597268 92-93 20.954399726984768 27.724152074611347 28.868925451140047 22.45252274726384 94-95 20.679776216964445 27.75047422521853 28.56008561687729 23.009663940939735 96-97 20.36234182792288 27.239018075435546 28.61744226659105 23.781197830050534 98-99 20.986127337290846 27.217065803985758 28.159928787809847 23.63687807091355 100-101 21.480409826769957 26.461646495899103 28.85154447489263 23.206399202438305 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4528.0 1 2833.5 2 1671.5 3 2050.0 4 1545.5 5 1396.5 6 2418.0 7 4922.0 8 7640.0 9 8266.5 10 6460.0 11 4806.5 12 4645.0 13 4777.5 14 5015.5 15 5329.0 16 5359.0 17 5298.5 18 5375.0 19 5491.5 20 5727.5 21 6236.0 22 6931.0 23 7771.5 24 8757.0 25 9849.5 26 11369.5 27 13315.5 28 15149.5 29 16738.0 30 18498.0 31 20745.0 32 23102.5 33 25200.0 34 27269.5 35 28985.5 36 30836.0 37 33073.5 38 34936.5 39 35806.5 40 36567.5 41 39014.5 42 41410.5 43 43273.5 44 45479.0 45 47593.5 46 50434.5 47 59966.0 48 96501.5 49 97424.0 50 65181.0 51 55887.0 52 49081.5 53 46493.0 54 45120.5 55 41974.5 56 37219.0 57 32822.5 58 29608.5 59 26453.5 60 22892.0 61 19776.0 62 16523.5 63 12680.0 64 9322.0 65 7148.0 66 5591.0 67 4708.0 68 3683.5 69 2616.0 70 1993.0 71 1511.5 72 1203.0 73 981.5 74 773.0 75 616.5 76 451.5 77 281.5 78 173.0 79 123.5 80 84.0 81 51.5 82 45.0 83 36.5 84 29.5 85 21.0 86 14.0 87 12.0 88 11.0 89 8.5 90 4.0 91 2.5 92 5.0 93 3.5 94 1.0 95 1.5 96 3.5 97 3.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3862935895025036 2 0.1136570082645155 3 0.11423071161526178 4 0.12678844051493063 5 0.12908325391791578 6 0.07184040847678573 7 0.08178459988972148 8 0.07885233831924042 9 0.07878859350249083 10-11 0.07611131119900813 12-13 0.08050970355472971 14-15 0.07403960465464651 16-17 0.07579258711526018 18-19 0.08573677852819593 20-21 0.08382443402570829 22-23 0.09399173229726758 24-25 0.10776061271517859 26-27 0.10913112627529474 28-29 0.1029160066422099 30-31 0.0927487083706506 32-33 0.09102759831841173 34-35 0.3377519115476923 36-37 0.5267552932102209 38-39 0.5083330411695899 40-41 0.48226141111900833 42-43 0.5085242756198386 44-45 0.5171616982894078 46-47 0.5153130986036698 48-49 0.5211776217446319 50-51 0.5199345978180149 52-53 0.5193290220588939 54-55 0.5139425850435536 56-57 0.48640482420773157 58-59 0.4779267635800364 60-61 0.4261340999709961 62-63 0.49797450844778185 64-65 0.525193545199856 66-67 0.5346596504871698 68-69 0.5487791273972035 70-71 0.5440301385493592 72-73 0.5307712166654449 74-75 0.5348827573457934 76-77 0.5123489646248139 78-79 0.48854027556884283 80-81 0.4941179470344318 82-83 0.5071218896513476 84-85 0.5017354526360075 86-87 0.5028509869291253 88-89 0.5127314335253115 90-91 0.5295600651472028 92-93 0.5356158227384136 94-95 0.5300381512728246 96-97 0.5322692198590602 98-99 0.532141730225561 100-101 0.5098310443632053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1568755.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.89807084452715 #Duplication Level Percentage of deduplicated Percentage of total 1 76.33180846997544 39.61473603665663 2 13.430689933087617 13.940537952765176 3 4.575016207277888 7.123035457205031 4 1.941271205604193 4.029929222275482 5 1.0612235579393445 2.7537727695908636 6 0.6269787106579253 1.9523391326241182 7 0.4065344555172149 1.4768847781221583 8 0.2770219002195656 1.1501521762464435 9 0.2160344007666981 1.009059177226056 >10 1.0109729787857606 9.467541872526986 >50 0.06788590655227277 2.399389360158604 >100 0.04520184334948921 4.393873826975874 >500 0.004557051577146541 1.6611402159969417 >1k 0.003941233796451062 4.465672620071199 >5k 4.926542245563827E-4 2.0155738968382164 >10k+ 3.694906684172871E-4 2.5463615047202617 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15914 1.0144350137529443 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12456 0.7940054374328688 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11467 0.7309618136675262 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9360 0.5966514847761442 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8319 0.530293130539823 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7508 0.47859608415590704 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6346 0.4045246070928858 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4314 0.27499513945772286 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3898 0.2484772956898942 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3501 0.22317060344030776 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3409 0.21730608029934564 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3332 0.21239772940962737 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3045 0.1941029670024956 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2951 0.18811095422803434 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2866 0.18269264480431935 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2863 0.18250141035407058 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2808 0.17899544543284324 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2437 0.15534611841874607 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2135 0.1360951837603705 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2112 0.13462905297512998 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2079 0.13252547402239356 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1912 0.12188008962521234 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1909 0.1216888551749636 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1873 0.11939404177197842 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1726 0.11002355370978897 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1711 0.10906738145854515 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1647 0.10498771318657152 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1642 0.10466898910282357 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1611 0.10269289978358635 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1595 0.10167298271559293 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.374481674958805E-5 0.0 14-15 0.0 0.0 0.0 1.274896334991761E-4 3.1872408374794026E-5 16-17 0.0 0.0 0.0 2.549792669983522E-4 6.374481674958805E-5 18-19 0.0 0.0 0.0 3.1872408374794027E-4 6.374481674958805E-5 20-21 0.0 0.0 0.0 6.055757591210864E-4 6.374481674958805E-5 22-23 0.0 0.0 0.0 0.001243023926616967 9.561722512438207E-5 24-25 0.0 0.0 0.0 0.0021035789527364054 1.274896334991761E-4 26-27 0.0 0.0 0.0 0.0034740925128525484 1.274896334991761E-4 28-29 0.0 0.0 0.0 0.00736252633457742 1.274896334991761E-4 30-31 0.0 0.0 0.0 0.017561697014511508 1.274896334991761E-4 32-33 0.0 0.0 0.0 0.033593518427032905 1.274896334991761E-4 34-35 0.0 0.0 0.0 0.057880293608625955 1.274896334991761E-4 36-37 0.0 0.0 0.0 0.10039808638060117 1.274896334991761E-4 38-39 0.0 0.0 0.0 0.15815089035572794 1.274896334991761E-4 40-41 0.0 0.0 0.0 0.22246941045606228 1.274896334991761E-4 42-43 0.0 0.0 0.0 0.28822218893326235 1.274896334991761E-4 44-45 0.0 0.0 0.0 0.3643972449490201 1.274896334991761E-4 46-47 0.0 0.0 0.0 0.46167183530889144 1.274896334991761E-4 48-49 0.0 0.0 0.0 0.575775057290654 1.274896334991761E-4 50-51 0.0 0.0 0.0 0.6928105408428977 1.274896334991761E-4 52-53 0.0 0.0 0.0 0.8083798936099008 1.5936204187397014E-4 54-55 0.0 0.0 0.0 0.9301006211932392 1.9123445024876414E-4 56-57 0.0 0.0 0.0 1.0807614955808904 1.9123445024876414E-4 58-59 0.0 0.0 0.0 1.267470063840434 1.9123445024876414E-4 60-61 0.0 0.0 0.0 1.4907681569142408 1.9123445024876414E-4 62-63 0.0 0.0 0.0 1.7008710729208831 1.9123445024876414E-4 64-65 0.0 0.0 0.0 1.9061293828545567 1.9123445024876414E-4 66-67 0.0 0.0 0.0 2.1508138619478503 1.9123445024876414E-4 68-69 0.0 0.0 0.0 2.4368687271116265 1.9123445024876414E-4 70-71 0.0 0.0 0.0 2.7597043515399156 1.9123445024876414E-4 72-73 0.0 0.0 0.0 3.0897431402609077 1.9123445024876414E-4 74-75 0.0 0.0 0.0 3.426921348457854 1.9123445024876414E-4 76-77 0.0 0.0 0.0 3.774681196235231 1.9123445024876414E-4 78-79 0.0 0.0 0.0 4.1852615609193276 1.9123445024876414E-4 80-81 0.0 0.0 0.0 4.641865683296627 1.9123445024876414E-4 82-83 0.0 0.0 0.0 5.12428645645751 1.9123445024876414E-4 84-85 0.0 0.0 0.0 5.643105519982406 1.9123445024876414E-4 86-87 0.0 0.0 0.0 6.182928500626293 1.9123445024876414E-4 88-89 0.0 0.0 0.0 6.725651870432285 1.9123445024876414E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1535 0.0 29.313023 2 GAGTACT 10705 0.0 23.086388 12-13 GTAAGGT 475 0.0 21.93686 4 AGTACTT 10950 0.0 21.813192 12-13 CATGGGG 3865 0.0 21.813023 4 GTACATG 15465 0.0 21.70687 1 GTACTTT 11530 0.0 21.476591 14-15 TACATGG 15515 0.0 21.308308 2 TAAGGTG 575 0.0 20.5929 5 CGGACCG 115 0.0037863974 20.5929 5 ATGGGAG 2890 0.0 20.486017 5 GTACCTG 2275 0.0 20.453726 1 ACATGGG 15675 0.0 20.39584 3 ACCTGGG 2235 0.0 20.132208 3 TAACGCA 170 8.13385E-5 19.50269 4 ACTTTTT 12910 0.0 19.438215 16-17 TACCCGG 195 1.0589878E-5 19.43125 2 TACTTTT 12230 0.0 19.395645 14-15 GTACCCG 200 1.289457E-5 18.992744 1 GTACACG 275 2.8518116E-8 18.992744 1 >>END_MODULE