##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939156_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1568755 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46468824003748 34.0 31.0 34.0 31.0 34.0 2 32.659909291125764 34.0 31.0 34.0 31.0 34.0 3 32.75371106386912 34.0 31.0 34.0 31.0 34.0 4 36.20968028787159 37.0 35.0 37.0 35.0 37.0 5 36.19166217796916 37.0 35.0 37.0 35.0 37.0 6 36.25713192945999 37.0 37.0 37.0 35.0 37.0 7 36.215742419944476 37.0 37.0 37.0 35.0 37.0 8 36.20224509244592 37.0 37.0 37.0 35.0 37.0 9 37.918791015805525 39.0 38.0 39.0 35.0 39.0 10-11 37.83757406350897 39.0 38.0 39.0 35.0 39.0 12-13 37.74481611213988 39.0 38.0 39.0 35.0 39.0 14-15 39.04424559602997 40.0 38.0 41.0 36.0 41.0 16-17 39.16635134230648 40.5 38.5 41.0 36.0 41.0 18-19 39.283109535905865 41.0 39.0 41.0 36.0 41.0 20-21 39.27373777294734 41.0 39.0 41.0 36.0 41.0 22-23 39.202405729384125 41.0 39.0 41.0 35.5 41.0 24-25 39.1084073038811 41.0 39.0 41.0 35.0 41.0 26-27 38.9063735255027 40.0 38.0 41.0 35.0 41.0 28-29 38.735430644045756 40.0 38.0 41.0 35.0 41.0 30-31 38.49013835812475 40.0 38.0 41.0 34.5 41.0 32-33 38.293532450892585 40.0 37.5 41.0 34.0 41.0 34-35 38.143987429522134 40.0 37.0 41.0 34.0 41.0 36-37 37.9470984953036 40.0 37.0 41.0 33.5 41.0 38-39 37.766382258542606 40.0 37.0 41.0 33.0 41.0 40-41 37.529368193248786 40.0 36.5 41.0 33.0 41.0 42-43 37.29691698193791 40.0 36.0 41.0 33.0 41.0 44-45 36.92534908255273 40.0 36.0 41.0 31.5 41.0 46-47 36.41502720310055 40.0 35.5 41.0 30.5 41.0 48-49 35.89934183476706 39.5 35.0 41.0 28.5 41.0 50-51 35.5716969826391 39.0 35.0 41.0 27.5 41.0 52-53 35.24833928816163 39.0 35.0 40.5 27.0 41.0 54-55 35.01005510739408 39.0 35.0 40.0 26.0 41.0 56-57 34.789460113274544 38.0 35.0 40.0 25.5 41.0 58-59 34.46852886524665 38.0 34.0 40.0 25.0 41.0 60-61 34.06367151021033 37.5 34.0 40.0 23.0 41.0 62-63 33.64549340081784 37.0 33.5 40.0 22.0 41.0 64-65 33.163925533305076 36.0 33.0 39.0 20.5 41.0 66-67 32.73873294427747 36.0 33.0 39.0 19.5 40.0 68-69 32.2567982253443 35.0 32.0 38.5 17.5 40.0 70-71 31.73174013788004 35.0 31.5 37.5 12.5 40.0 72-73 31.151276968041536 35.0 31.0 37.0 7.5 39.0 74-75 30.61821635628253 35.0 30.5 36.5 6.0 39.0 76-77 28.626408202683017 32.5 28.0 34.5 2.0 36.5 78-79 29.585060764746565 34.0 29.5 35.0 2.0 37.0 80-81 29.62329331221255 34.0 30.0 35.0 2.0 37.0 82-83 29.258215272620646 34.0 29.5 35.0 2.0 36.5 84-85 28.80502596007662 34.0 29.0 35.0 2.0 36.0 86-87 28.107750094820418 34.0 28.0 35.0 2.0 36.0 88-89 27.399995856586912 33.0 26.5 35.0 2.0 35.0 90-91 26.807782923401042 33.0 25.0 35.0 2.0 35.0 92-93 26.38889182823322 32.0 25.0 34.0 2.0 35.0 94-95 25.86783468419224 32.0 24.0 34.0 2.0 35.0 96-97 25.056662767608707 32.0 19.0 34.0 2.0 35.0 98-99 23.873619526312268 31.5 2.0 34.0 2.0 35.0 100-101 18.20309194233644 23.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23109601133346303 1101 2 0.17697007266699671 1101 3 0.21865772536257566 1101 4 0.18301367911620758 1101 5 0.20383304858830087 1101 6 0.19285026426229024 1101 7 0.15968527969904045 1101 8 0.23990997751665333 1101 9 0.28871889471562184 1101 10-11 0.3236753120579863 1101 12-13 0.30935717250808636 1101 14-15 0.3302511227451319 1101 16-17 0.3937531419796869 1101 18-19 0.3679007835076433 1101 20-21 0.38964576841603105 1101 22-23 0.40078309778337484 1101 24-25 0.3808354907346754 1101 26-27 0.22284158961900857 1101 28-29 0.2874809652251642 1101 30-31 0.3807353490031744 1101 32-33 0.42001139285554245 1101 34-35 0.48008065539448097 1101 36-37 0.37773171010079665 1101 38-39 0.4491488881699084 1101 40-41 0.5982491107316861 1101 42-43 0.3812658910842117 1101 44-45 0.2786322066226674 1101 46-47 0.2215415805829295 1101 48-49 0.2553190462027928 1101 50-51 0.3569967784782335 1101 52-53 0.29808160141809736 1101 54-55 0.21643956632754424 1101 56-57 0.3771342911296145 1101 58-59 0.20169873920282555 1101 60-61 0.11310490287620922 1101 62-63 0.06968370831391724 1101 64-65 -0.025022880065080244 1101 66-67 0.05408713959548095 1101 68-69 0.13888520487367373 1101 70-71 0.15267135679694732 1101 72-73 0.22343183967947766 1101 74-75 0.254700498002542 1101 76-77 0.42441052227906795 1101 78-79 0.26588473214617636 1101 80-81 0.3820463142063346 1101 82-83 0.27112408157871926 1101 84-85 0.19799473953020197 1101 86-87 0.37078374117180246 1101 88-89 0.18058432737844043 1101 90-91 0.24630932363456992 1101 92-93 0.29567645224000927 1101 94-95 0.147406229447963 1101 96-97 0.2617742147839053 1101 98-99 0.3713256013851982 1101 100-101 -0.5810752558612116 1102 1 0.17668460096722072 1102 2 0.18254429448465714 1102 3 0.15181056858382647 1102 4 0.08477485210114821 1102 5 0.10186840590964152 1102 6 0.11053985696594282 1102 7 0.1419374571817329 1102 8 0.10928109552907017 1102 9 0.1806858576627235 1102 10-11 0.13226041682702316 1102 12-13 0.10831204774903114 1102 14-15 0.22172670191983457 1102 16-17 0.24622367010847768 1102 18-19 0.20476806415303628 1102 20-21 0.2078114623547762 1102 22-23 0.23055571147652643 1102 24-25 0.0931579009581398 1102 26-27 0.20497663816616551 1102 28-29 0.1453661967165587 1102 30-31 0.10061871089365582 1102 32-33 0.14993047349380362 1102 34-35 0.26164467249307677 1102 36-37 0.355340004168049 1102 38-39 0.2831509719494463 1102 40-41 0.22424582219738198 1102 42-43 0.2952547671818735 1102 44-45 0.23844805762574595 1102 46-47 0.377591345878848 1102 48-49 0.2988642378926656 1102 50-51 0.3773398435528392 1102 52-53 0.3257000466597617 1102 54-55 0.2360285441600567 1102 56-57 0.33951136152298744 1102 58-59 0.2510903380462608 1102 60-61 0.045669870392458733 1102 62-63 -0.17325062808930625 1102 64-65 -0.007760305994970906 1102 66-67 -0.071587746175922 1102 68-69 0.14463456233779937 1102 70-71 0.2804724116266719 1102 72-73 -0.005415232320238772 1102 74-75 0.0381469444221878 1102 76-77 0.22541626940550685 1102 78-79 0.30059505555589894 1102 80-81 0.24658893129064552 1102 82-83 0.3323668976706742 1102 84-85 0.0935380393088856 1102 86-87 0.12736304080769756 1102 88-89 0.028949765195246613 1102 90-91 0.28650407001138234 1102 92-93 0.13069030691980998 1102 94-95 0.3541270877688021 1102 96-97 0.14110965866910163 1102 98-99 0.0734053650747839 1102 100-101 -0.3271386081136569 1103 1 0.08813514322600469 1103 2 0.15262267269457652 1103 3 0.17232057232354236 1103 4 0.06991727294382599 1103 5 0.1498224697341186 1103 6 0.16787827007987488 1103 7 0.13713036418746327 1103 8 0.2194390985208443 1103 9 0.1640674956582302 1103 10-11 0.16920374455078502 1103 12-13 0.21981985440573482 1103 14-15 0.15287113285974385 1103 16-17 0.2929798562216632 1103 18-19 0.2549425842228459 1103 20-21 0.2594545999763156 1103 22-23 0.2438005382030326 1103 24-25 0.25888963867939907 1103 26-27 0.18323564464833453 1103 28-29 0.24398756607582328 1103 30-31 0.2321242581387395 1103 32-33 0.2507917767847516 1103 34-35 0.3353735586810558 1103 36-37 0.3413978762697738 1103 38-39 0.40443657716011927 1103 40-41 0.24447339948483915 1103 42-43 0.2910417436216619 1103 44-45 0.5005826562295823 1103 46-47 0.5270533721815198 1103 48-49 0.4399917622008118 1103 50-51 0.4191525928672277 1103 52-53 0.3483701258170768 1103 54-55 0.365054581931183 1103 56-57 0.41518996411586073 1103 58-59 0.40167233131477076 1103 60-61 0.5774730366851557 1103 62-63 0.4513208628107179 1103 64-65 0.5833991617188161 1103 66-67 0.44225309775328014 1103 68-69 0.2613370679473732 1103 70-71 0.31739041835816195 1103 72-73 0.41025222903603265 1103 74-75 0.3125057389871344 1103 76-77 0.33519250974457293 1103 78-79 0.3727248236935168 1103 80-81 0.6631484706823194 1103 82-83 0.43888257331265734 1103 84-85 0.2951398842353363 1103 86-87 0.38645522480171124 1103 88-89 0.6006373898524409 1103 90-91 0.687750252384852 1103 92-93 0.5988384464112109 1103 94-95 0.5245938510447914 1103 96-97 0.332752562558408 1103 98-99 0.2121098651994373 1103 100-101 -0.27517603643577004 1104 1 0.04972108275190834 1104 2 0.07041482593506032 1104 3 0.16419928123220728 1104 4 0.11710787843488646 1104 5 0.0684097144101159 1104 6 0.08061805658389432 1104 7 0.11177048351547114 1104 8 -0.005293710037854282 1104 9 0.07839103572248263 1104 10-11 0.18404634430690692 1104 12-13 0.18624197509246443 1104 14-15 0.15875234491342383 1104 16-17 0.25776919985332825 1104 18-19 0.1724478294689078 1104 20-21 0.2537654635827735 1104 22-23 0.17695017183947215 1104 24-25 0.23623676574187158 1104 26-27 0.16061732925635397 1104 28-29 0.2086027825399981 1104 30-31 0.22338869997811628 1104 32-33 0.21089227762806217 1104 34-35 0.28825876547983853 1104 36-37 0.2686445989285957 1104 38-39 0.28358482206542845 1104 40-41 0.27783750421402686 1104 42-43 0.10940808789410994 1104 44-45 0.2728682547876957 1104 46-47 0.15315439165589595 1104 48-49 0.06674696404902392 1104 50-51 0.09024512565135012 1104 52-53 0.10362528373166668 1104 54-55 0.2812182443237887 1104 56-57 0.23221713652639409 1104 58-59 0.1152877899496616 1104 60-61 0.14732937373533872 1104 62-63 0.08829170374313833 1104 64-65 0.13210145891156344 1104 66-67 0.161448394498116 1104 68-69 0.1675444067913432 1104 70-71 0.1754081975456252 1104 72-73 0.16667189586914333 1104 74-75 0.09918656442491525 1104 76-77 0.1888236237512544 1104 78-79 0.3884736883843871 1104 80-81 0.5006428182465754 1104 82-83 0.4192072749390583 1104 84-85 0.2596589289254787 1104 86-87 0.38118774404142286 1104 88-89 0.4491799722494143 1104 90-91 0.4987697696293374 1104 92-93 0.16824339226569762 1104 94-95 0.35074689435906237 1104 96-97 0.06064698470266805 1104 98-99 0.08514383553460192 1104 100-101 -0.31216171836062045 1105 1 -0.06151782161020236 1105 2 0.037334296149658996 1105 3 0.021812441710700625 1105 4 0.03985518843148128 1105 5 -0.05965548686425137 1105 6 0.0527091460102298 1105 7 0.045007304001579485 1105 8 0.04422739922537744 1105 9 0.12642137214823634 1105 10-11 0.027932973437089004 1105 12-13 -0.024743958744473105 1105 14-15 -0.01207439281876077 1105 16-17 0.14306484976215472 1105 18-19 0.01559057497554761 1105 20-21 -0.030703148034838534 1105 22-23 0.0715230774438993 1105 24-25 0.04913525693549303 1105 26-27 0.13123267379720005 1105 28-29 0.17536796578082914 1105 30-31 0.04704520107014787 1105 32-33 0.16554142504224956 1105 34-35 0.21319364435454702 1105 36-37 0.08611860057164478 1105 38-39 0.1225953817063612 1105 40-41 0.09304629256035213 1105 42-43 0.15983237707197873 1105 44-45 0.10026020381288703 1105 46-47 0.2736510153760179 1105 48-49 0.3738767254051538 1105 50-51 0.23306276646326296 1105 52-53 0.11489574679296055 1105 54-55 0.11143594925496103 1105 56-57 0.2020810834677107 1105 58-59 0.10741214124305998 1105 60-61 0.09109527831786579 1105 62-63 0.050078126489438546 1105 64-65 0.2538083336986645 1105 66-67 0.05184403038086316 1105 68-69 -0.021967585514353516 1105 70-71 0.16339886017812333 1105 72-73 -0.16892932239682779 1105 74-75 -0.0675796206793784 1105 76-77 -0.2595779030886689 1105 78-79 -0.2050617876009433 1105 80-81 0.04930132306553858 1105 82-83 -0.09084842866965559 1105 84-85 -0.14658017580932992 1105 86-87 0.1560905632852183 1105 88-89 0.045654414712398506 1105 90-91 0.10407764227245053 1105 92-93 0.07503502470202861 1105 94-95 0.0782420353212494 1105 96-97 -0.11288478438783756 1105 98-99 -0.1750982146787976 1105 100-101 -0.953388399988448 1106 1 0.01889690255451626 1106 2 0.019201040516399814 1106 3 -0.07532236792410885 1106 4 -0.06820133837504727 1106 5 0.13311840590964152 1106 6 0.04977596807704998 1106 7 0.09729460003887169 1106 8 0.09449934949732608 1106 9 0.1850509370278033 1106 10-11 0.052374504128614774 1106 12-13 0.05764240489189376 1106 14-15 0.04526340826903663 1106 16-17 0.04218101137831809 1106 18-19 0.09980774669272563 1106 20-21 0.16847614489445562 1106 22-23 0.1788196003654221 1106 24-25 0.13050909143432676 1106 26-27 0.12873655880108714 1106 28-29 0.13551996655782972 1106 30-31 0.23965640930636312 1106 32-33 0.23363583063665772 1106 34-35 -0.042298779887879334 1106 36-37 -0.07615801170496894 1106 38-39 0.12652894813992788 1106 40-41 0.19401268727674648 1106 42-43 0.1946347274993343 1106 44-45 0.4542714703241586 1106 46-47 0.3665050363550364 1106 48-49 0.33140392043234357 1106 50-51 0.2792991689496631 1106 52-53 0.2867367530089666 1106 54-55 0.2821842981283069 1106 56-57 0.2518625519991744 1106 58-59 0.26006851264943265 1106 60-61 0.21476709261467875 1106 62-63 0.2585450068313264 1106 64-65 0.3200670749574144 1106 66-67 0.37692415858598594 1106 68-69 0.22895995916319833 1106 70-71 0.27045257035683434 1106 72-73 0.3115242518067447 1106 74-75 0.11552789680314035 1106 76-77 0.2899996027388383 1106 78-79 0.40629942063526414 1106 80-81 0.41754627256048593 1106 82-83 0.2854918976706742 1106 84-85 0.2378336742295204 1106 86-87 0.17795827890293126 1106 88-89 0.35680194773492957 1106 90-91 0.6977887922336024 1106 92-93 0.5694551878721903 1106 94-95 0.41888403221324566 1106 96-97 0.13751342851037407 1106 98-99 0.30827639682081553 1106 100-101 -0.4783786874787346 1107 1 -0.08985123316509203 1107 2 -0.11009612577741024 1107 3 -0.07432497791665327 1107 4 -0.0934390341244864 1107 5 -0.014980028094832676 1107 6 -0.005238946166052472 1107 7 0.0555859870634805 1107 8 -0.0206386072513709 1107 9 -0.06783403687227718 1107 10-11 -0.05047318416370672 1107 12-13 -0.08258410517522208 1107 14-15 -0.06180687510158123 1107 16-17 -0.012060226503862737 1107 18-19 -0.03968703332218837 1107 20-21 -0.05526080514282938 1107 22-23 -0.07073064199103385 1107 24-25 -0.03370371617193513 1107 26-27 -0.027536744703766658 1107 28-29 -0.08522714380757179 1107 30-31 -0.045799873318543405 1107 32-33 -0.12483732372575673 1107 34-35 -0.2277154465545408 1107 36-37 -0.25553907061622283 1107 38-39 -0.2639911860882549 1107 40-41 -0.162088170292229 1107 42-43 -0.20312580568486283 1107 44-45 -0.32547312326272504 1107 46-47 -0.12852432606106845 1107 48-49 -0.2465696067861458 1107 50-51 -0.32231809428672875 1107 52-53 -0.471542982263216 1107 54-55 -0.4175803364727386 1107 56-57 -0.41864919296726555 1107 58-59 -0.40470124872267377 1107 60-61 -0.4592238656254395 1107 62-63 -0.4894159364945523 1107 64-65 -0.4807718512170922 1107 66-67 -0.42990609868471097 1107 68-69 -0.4108843734244232 1107 70-71 -0.4799272767721767 1107 72-73 -0.5930106102365045 1107 74-75 -0.645734687089476 1107 76-77 -0.616054647820448 1107 78-79 -0.5905243116540717 1107 80-81 -0.5274527952993182 1107 82-83 -0.6320696235821259 1107 84-85 -0.6003817433692866 1107 86-87 -0.5006630857503076 1107 88-89 -0.6913827092374802 1107 90-91 -0.2870872331206087 1107 92-93 -0.07745476738507762 1107 94-95 -0.1303842377345532 1107 96-97 -0.4984951471794119 1107 98-99 -0.26680516544615074 1107 100-101 -0.8506475167106515 1108 1 -0.12399330908569084 1108 2 -0.05811377429841258 1108 3 -0.07115570125744597 1108 4 -0.03708361350732048 1108 5 0.03920306199429291 1108 6 0.03654845484953739 1108 7 -0.012659103664830695 1108 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-0.6841421683270674 2316 100-101 0.46935312706962407 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 15.0 5 54.0 6 107.0 7 227.0 8 490.0 9 920.0 10 1650.0 11 2576.0 12 4199.0 13 6144.0 14 7462.0 15 10456.0 16 14580.0 17 22907.0 18 22896.0 19 13170.0 20 10806.0 21 11148.0 22 11297.0 23 11766.0 24 13025.0 25 14813.0 26 17223.0 27 20427.0 28 24722.0 29 30141.0 30 36430.0 31 44703.0 32 56388.0 33 72945.0 34 104937.0 35 161394.0 36 243843.0 37 352800.0 38 212955.0 39 9130.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.280899149733244 18.83102084504301 14.858094690601998 20.029985314621747 2 15.275680396237782 21.985268572849172 39.917322972675784 22.821728058237266 3 20.940746005590423 27.288709836781393 29.83085312875497 21.939691028873217 4 12.616629110345464 17.170495074119284 37.23302873935063 32.97984707618461 5 12.69777626206769 39.39780271616664 32.61299565579074 15.291425365974929 6 29.101357445872683 36.36558927302224 17.85052477920389 16.68252850190119 7 27.175690276684378 35.31647707895752 19.958948338013265 17.54888430634484 8 26.14965370628301 33.14398997931481 19.508718697310925 21.19763761709126 9 27.483004038234142 17.915225768204724 20.634452161108648 33.96731803245249 10-11 24.973529964844733 27.018718665438517 25.83940768316276 22.16834368655399 12-13 28.186874304783093 24.817163929357992 26.664488718761053 20.33147304709786 14-15 24.374355774017797 25.319475529133832 27.903837658558476 22.40233103828989 16-17 20.563155732388356 29.051671959220087 28.557222207879256 21.8279501005123 18-19 20.376221908456067 27.62786413429758 31.90335648332595 20.0925574739204 20-21 22.353139910311043 25.63016532218224 31.22887257729856 20.787822190208157 22-23 21.377461745141847 25.81591771819054 31.021733795270773 21.78488674139684 24-25 21.586214787727965 26.018364261816785 31.698415334212797 20.69700561624245 26-27 21.813375270567516 25.262901898936917 32.49573610141178 20.427986729083788 28-29 20.66231523160571 26.20247463831976 32.67327679378768 20.461933336286844 30-31 20.71378258555351 26.58050492269347 31.994989657403483 20.710722834349532 32-33 20.622379091183486 26.57799547347912 31.60542328324795 21.194202152089446 34-35 21.531283499934496 25.86575067107451 31.525759347786657 21.077206481204335 36-37 21.909167568636015 25.54173155798135 32.14239372835074 20.406707145031888 38-39 21.922494399695495 25.548992423370585 32.20933008977383 20.319183087160088 40-41 21.00234730410657 26.210245007038086 32.334209694200204 20.453197994655135 42-43 20.65398769290597 26.891845155655574 31.125600253728226 21.32856689771023 44-45 21.029253052385716 27.03610844965643 30.474676019687752 21.4599624782701 46-47 21.51755783683957 27.021687639470287 29.5750795982682 21.885674925421945 48-49 21.956369023562157 26.80509108804604 29.618691121704444 21.61984876668736 50-51 21.91455196931556 27.594704805057297 29.636180049887074 20.85456317574007 52-53 21.605476954655124 27.897632198781835 29.390440189832063 21.106450656730974 54-55 20.619535873989246 27.4279285165625 29.878151782783164 22.074383826665095 56-57 21.594182897440547 28.12016036936332 28.71729051122436 21.568366221971775 58-59 21.80495510369561 27.157865682088524 29.138166894664842 21.899012319551026 60-61 21.394576318294042 26.910754229741197 30.14786643573426 21.546803016230502 62-63 22.012229618497734 26.894578903669046 29.68342276328182 21.409768714551397 64-65 21.45077581483586 27.39153397498766 29.86497465585478 21.2927155543217 66-67 21.02426786671048 27.444053928026058 29.62484394128846 21.906834263975004 68-69 20.724460618083913 28.07986125830759 29.391449638403987 21.80422848520451 70-71 21.248298408001524 28.02384571425111 28.545247606137746 22.18260827160962 72-73 21.118434682279897 27.43825517687593 28.714936366736683 22.728373774107492 74-75 20.94900095935949 28.305885877654575 28.44692128471304 22.298191878272895 76-77 21.362010621745707 27.233512829584694 29.04269339447627 22.361783154193333 78-79 21.055503913925516 27.287214169953245 29.488996507495685 22.168285408625554 80-81 20.929378669728134 28.37302637072772 28.81952921662958 21.87806574291457 82-83 20.744252607959815 27.976484537101076 28.666554050823745 22.612708804115364 84-85 20.727965807280295 27.545442086240364 28.59761403150906 23.12897807497028 86-87 21.06199880093513 27.472943665966948 28.781896226327802 22.68316130677012 88-89 21.305026967335113 26.936222652561497 28.726149512667448 23.032600867435942 90-91 20.647654446018553 27.061974299701102 29.273572531037082 23.01679872324326 92-93 20.484141883213123 27.753027081985397 28.871111167773172 22.89171986702831 94-95 20.178612976532346 27.72539370392445 28.623366937475897 23.472626382067308 96-97 19.780048509805546 27.275195935630485 28.682458382602764 24.262297171961205 98-99 20.435409664685793 27.194952677366253 28.18239333483134 24.187244323116616 100-101 20.986231075697212 26.09625498007968 29.03665338645418 23.880860557768923 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3331.0 1 2221.0 2 1624.5 3 2009.0 4 1471.5 5 1280.0 6 2293.5 7 4818.0 8 7368.0 9 7883.0 10 6327.0 11 4684.0 12 4358.5 13 4551.5 14 4749.0 15 4863.0 16 4915.0 17 4986.5 18 5105.5 19 5353.5 20 5707.5 21 6208.5 22 6892.5 23 7761.5 24 8703.5 25 9754.5 26 11378.0 27 13338.0 28 15276.0 29 17047.0 30 18710.5 31 20890.5 32 23374.0 33 25540.0 34 27613.0 35 29054.5 36 30872.5 37 33344.5 38 34880.5 39 35729.0 40 36747.0 41 39024.5 42 41631.0 43 43738.5 44 45849.5 45 48168.0 46 51039.5 47 61045.5 48 96869.0 49 97939.0 50 66891.5 51 56906.5 52 49204.5 53 46208.5 54 45194.0 55 42092.5 56 37126.5 57 32659.5 58 29457.5 59 26432.5 60 22895.5 61 19548.5 62 16266.0 63 12455.0 64 9040.0 65 6941.0 66 5370.5 67 4459.0 68 3429.0 69 2342.0 70 1747.5 71 1318.5 72 1061.0 73 898.5 74 704.0 75 569.0 76 396.5 77 217.0 78 118.5 79 66.5 80 37.0 81 20.5 82 10.5 83 6.5 84 4.5 85 3.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4243492451020076 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.649378009950566E-4 16-17 0.002645409895107904 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.561722512438207E-5 26-27 0.003920306230099665 28-29 3.1872408374794026E-5 30-31 0.0 32-33 3.1872408374794026E-5 34-35 0.012015897957297347 36-37 0.02725090916044889 38-39 0.019984000050995855 40-41 0.009370488062189443 42-43 0.009402360470564237 44-45 0.004079668271973635 46-47 0.006151374816335247 48-49 0.0033784752877281665 50-51 7.649378009950566E-4 52-53 0.0 54-55 0.0 56-57 9.561722512438207E-5 58-59 0.0018485996857380534 60-61 0.002677282303482698 62-63 0.0014342583768657312 64-65 0.003155368429104608 66-67 0.0021991961778607878 68-69 0.00538643701534019 70-71 2.2310685862355817E-4 72-73 0.0 74-75 0.0 76-77 0.09552160789925769 78-79 0.016127438637645774 80-81 5.099585339967044E-4 82-83 0.0 84-85 0.0 86-87 0.10839806088267447 88-89 0.4258153758872482 90-91 0.4253054173532515 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.593620418739701E-4 100-101 3.187240837479402E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1568755.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.80991811676399 #Duplication Level Percentage of deduplicated Percentage of total 1 75.58533830213837 38.40484849959555 2 13.807200154222055 14.030854185155873 3 4.661970038543402 7.10622947763592 4 2.0705835935643324 4.208247313716745 5 1.1151268172828164 2.8329751137973767 6 0.6581341905364394 2.006384659859954 7 0.41754282646504604 1.485072177605183 8 0.28767538345067645 1.1693410141870042 9 0.20166604846531822 0.9221971868508758 >10 1.0628702704720878 9.704908956803575 >50 0.07417675073061085 2.5792520913422723 >100 0.04767825303607568 4.484963892305851 >500 0.005018685546439895 1.7526081384668997 >1k 0.0041404155758129136 4.614780682943416 >5k 5.018685546439895E-4 2.1165554635981207 >10k+ 3.764014159829922E-4 2.5807811461354393 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15915 1.0144987585696938 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13057 0.8323160722993711 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11511 0.733766585604508 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9864 0.6287788724179365 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8823 0.5624205181816153 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8313 0.5299106616393254 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6201 0.3952816086641954 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4086 0.26046132123881677 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3823 0.2436964344336751 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3759 0.2396167661617015 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3410 0.21736982511609526 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3360 0.21418258427861586 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3343 0.21309892239387285 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2983 0.19015078836402116 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2810 0.17912293506634241 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2796 0.17823050763184817 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2663 0.16975244700415298 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2638 0.16815882658541326 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2267 0.14450949957131612 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2213 0.14106727946683836 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2125 0.1354577355928746 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1925 0.122708772242957 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1900 0.12111515182421728 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1895 0.12079642774046936 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1864 0.11882033842123213 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1754 0.11180840857877743 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1722 0.10976857444279062 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1672 0.1065813336053112 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1656 0.1055614165373178 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1576 0.10046183119735076 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.274896334991761E-4 0.0 18-19 0.0 0.0 0.0 1.9123445024876414E-4 6.374481674958805E-5 20-21 0.0 0.0 0.0 4.1434130887232234E-4 6.374481674958805E-5 22-23 0.0 0.0 0.0 0.0010517894763682027 6.374481674958805E-5 24-25 0.0 0.0 0.0 0.0019123445024876414 6.374481674958805E-5 26-27 0.0 0.0 0.0 0.0032191132458541964 6.374481674958805E-5 28-29 0.0 0.0 0.0 0.00720316429270345 6.374481674958805E-5 30-31 0.0 0.0 0.0 0.0175935694228863 6.374481674958805E-5 32-33 0.0 0.0 0.0 0.03375288046890687 6.374481674958805E-5 34-35 0.0 0.0 0.0 0.0581352728756243 6.374481674958805E-5 36-37 0.0 0.0 0.0 0.10113115177322143 6.374481674958805E-5 38-39 0.0 0.0 0.0 0.15971263836609284 6.374481674958805E-5 40-41 0.0 0.0 0.0 0.22533792720979376 6.374481674958805E-5 42-43 0.0 0.0 0.0 0.2918875158963637 9.561722512438207E-5 44-45 0.0 0.0 0.0 0.36911436138848963 1.5936204187397014E-4 46-47 0.0 0.0 0.0 0.4669626550991073 1.9123445024876414E-4 48-49 0.0 0.0 0.0 0.5818308148818649 1.9123445024876414E-4 50-51 0.0 0.0 0.0 0.6995674914183541 1.9123445024876414E-4 52-53 0.0 0.0 0.0 0.8168260818292212 1.9123445024876414E-4 54-55 0.0 0.0 0.0 0.9398854505643011 1.9123445024876414E-4 56-57 0.0 0.0 0.0 1.0922355625958164 2.2310685862355817E-4 58-59 0.0 0.0 0.0 1.2801234099652272 2.549792669983522E-4 60-61 0.0 0.0 0.0 1.5062900197927656 2.549792669983522E-4 62-63 0.0 0.0 0.0 1.718241535485146 2.549792669983522E-4 64-65 0.0 0.0 0.0 1.9263046173558012 2.549792669983522E-4 66-67 0.0 0.0 0.0 2.1730608029934566 2.549792669983522E-4 68-69 0.0 0.0 0.0 2.461569843602092 2.549792669983522E-4 70-71 0.0 0.0 0.0 2.786827771066865 2.8685167537314627E-4 72-73 0.0 0.0 0.0 3.1183645629814727 3.1872408374794027E-4 74-75 0.0 0.0 0.0 3.458698139607523 3.1872408374794027E-4 76-77 0.0 0.0 0.0 3.8089759076465093 3.1872408374794027E-4 78-79 0.0 0.0 0.0 4.2208311686655975 3.1872408374794027E-4 80-81 0.0 0.0 0.0 4.678773932194638 3.1872408374794027E-4 82-83 0.0 0.0 0.0 5.16125845017227 3.1872408374794027E-4 84-85 0.0 0.0 0.0 5.680236875739041 3.1872408374794027E-4 86-87 0.0 0.0 0.0 6.218020022246941 3.1872408374794027E-4 88-89 0.0 0.0 0.0 6.7582573441997 3.1872408374794027E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1455 0.0 29.676987 2 GTACACG 310 0.0 26.127035 1 GTAAGCG 365 0.0 24.700294 94-95 GAGTACT 11950 0.0 22.515636 12-13 ATGGGCG 275 5.2750693E-11 22.431177 5 AGTACTT 12160 0.0 21.365824 12-13 GTACATG 15385 0.0 21.274637 1 TACATGG 15380 0.0 21.19541 2 GTACTTT 12785 0.0 20.933771 14-15 ATGGGAT 3275 0.0 20.718872 5 GTACCTG 2130 0.0 20.354698 1 ACATGGG 15485 0.0 20.04047 3 TATACAG 885 0.0 19.301926 5 ACTTTTT 14220 0.0 19.020573 16-17 TACTTTT 13590 0.0 18.960485 14-15 GTCGGGA 355 8.0035534E-11 18.7129 2 CATGGGG 3925 0.0 18.617546 4 GCCGGTC 130 0.007609883 18.250217 8 ACGGTAG 130 0.007609883 18.250217 5 ACCTGGG 2295 0.0 17.987795 3 >>END_MODULE