##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939155_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 59890 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.307280013357826 33.0 31.0 34.0 31.0 34.0 2 32.48689263650025 34.0 31.0 34.0 31.0 34.0 3 32.569193521456 34.0 31.0 34.0 31.0 34.0 4 36.081449323760225 37.0 35.0 37.0 35.0 37.0 5 36.04059108365337 37.0 35.0 37.0 35.0 37.0 6 36.12693270996827 37.0 36.0 37.0 35.0 37.0 7 36.092068792786776 37.0 35.0 37.0 35.0 37.0 8 36.10921689764568 37.0 36.0 37.0 35.0 37.0 9 37.75035899148439 39.0 38.0 39.0 35.0 39.0 10-11 37.71349140090165 39.0 37.5 39.0 35.0 39.0 12-13 37.68286859241944 39.0 37.0 39.0 35.0 39.0 14-15 38.83431290699616 40.0 38.0 41.0 35.0 41.0 16-17 38.80652028719319 40.0 38.0 41.0 34.5 41.0 18-19 38.88052262481216 40.0 38.0 41.0 35.0 41.0 20-21 38.946009350475876 40.0 38.0 41.0 35.0 41.0 22-23 38.86983636667223 40.0 38.0 41.0 35.0 41.0 24-25 38.72454499916513 40.0 38.0 41.0 35.0 41.0 26-27 38.48415428285189 40.0 38.0 41.0 34.5 41.0 28-29 38.31655535147771 40.0 37.0 41.0 34.0 41.0 30-31 38.062923693437966 40.0 37.0 41.0 34.0 41.0 32-33 37.69936550342294 40.0 35.0 41.0 33.0 41.0 34-35 37.48230088495575 40.0 35.0 41.0 33.0 41.0 36-37 37.26901820003339 40.0 35.0 41.0 33.0 41.0 38-39 37.063274336283186 40.0 35.0 41.0 33.0 41.0 40-41 36.91043579896477 40.0 35.0 41.0 33.0 41.0 42-43 36.65200367340124 40.0 35.0 41.0 31.5 41.0 44-45 36.30072633160795 40.0 35.0 41.0 31.0 41.0 46-47 35.84552512940391 39.0 35.0 41.0 29.5 41.0 48-49 35.397094673568205 39.0 35.0 41.0 27.0 41.0 50-51 35.10711304057439 39.0 35.0 41.0 26.0 41.0 52-53 34.95106027717482 39.0 35.0 41.0 24.0 41.0 54-55 34.72548839539155 39.0 35.0 41.0 23.5 41.0 56-57 34.40354817164802 38.5 35.0 40.0 22.0 41.0 58-59 34.09424778761062 38.0 35.0 40.0 20.0 41.0 60-61 33.611888462180666 38.0 34.0 40.0 14.0 41.0 62-63 33.24107530472533 37.5 34.0 40.0 7.5 41.0 64-65 32.75511771581232 37.0 33.0 39.5 2.0 41.0 66-67 32.28162464518283 36.0 33.0 39.0 2.0 40.5 68-69 31.718066455167808 36.0 32.5 39.0 2.0 40.0 70-71 31.19354650192019 35.0 31.5 38.5 2.0 40.0 72-73 30.68556520287193 35.0 31.0 37.0 2.0 39.5 74-75 30.18430455835699 35.0 31.0 37.0 2.0 39.0 76-77 28.26030222073802 33.0 28.0 35.0 2.0 37.0 78-79 29.14513274336283 34.0 29.5 36.0 2.0 37.5 80-81 29.063215895808984 34.0 30.0 35.5 2.0 37.0 82-83 28.700350642845216 34.0 29.5 35.0 2.0 37.0 84-85 28.286516947737518 34.0 29.0 35.0 2.0 36.0 86-87 27.835949240273834 34.0 29.0 35.0 2.0 36.0 88-89 27.23600768074804 34.0 26.5 35.0 2.0 35.5 90-91 26.773342795124393 34.0 25.5 35.0 2.0 35.0 92-93 26.52708298547337 34.0 25.0 35.0 2.0 35.0 94-95 26.2237852730005 33.0 25.0 35.0 2.0 35.0 96-97 25.701452663215896 33.0 21.5 35.0 2.0 35.0 98-99 25.07178994823844 33.0 10.0 35.0 2.0 35.0 100-101 21.88499749540825 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5844971819528695 1101 2 0.12552239496150008 1101 3 0.38562895102685246 1101 4 0.27534544083733437 1101 5 0.32628986946912875 1101 6 0.3388266201038306 1101 7 0.03738332125177379 1101 8 0.3159518212255037 1101 9 0.17581037393539845 1101 10-11 0.713644086818455 1101 12-13 0.4792446886939956 1101 14-15 -0.039571222955117946 1101 16-17 0.31098483325624926 1101 18-19 -0.0669575657505419 1101 20-21 0.26492464746963407 1101 22-23 0.1668295810487166 1101 24-25 0.47944013424297793 1101 26-27 -0.12227807726518591 1101 28-29 0.07867922759353974 1101 30-31 0.06189835594564386 1101 32-33 0.46421845032990205 1101 34-35 0.3283931828041631 1101 36-37 0.6528655084875581 1101 38-39 0.09176643790200245 1101 40-41 0.9712935900954065 1101 42-43 0.7138460077376507 1101 44-45 1.107496576016615 1101 46-47 1.217030804372861 1101 48-49 1.6541014207555662 1101 50-51 2.1224729702886336 1101 52-53 1.9127698237731963 1101 54-55 2.46058709966416 1101 56-57 2.3840142669003583 1101 58-59 1.2106707080960177 1101 60-61 0.7255124729884486 1101 62-63 1.8880971440690288 1101 64-65 1.9363443741549844 1101 66-67 1.9704417092917765 1101 68-69 1.4073294821566655 1101 70-71 1.9180404167152467 1101 72-73 1.6317957850716844 1101 74-75 2.3216291039479735 1101 76-77 2.246415301175432 1101 78-79 1.5815705166002338 1101 80-81 0.8395137937766464 1101 82-83 0.3084993252483379 1101 84-85 1.3276470737335657 1101 86-87 1.7861594136526087 1101 88-89 2.374573519645768 1101 90-91 2.790667207978256 1101 92-93 1.9456811547646922 1101 94-95 1.5738143864474097 1101 96-97 1.2537378173636426 1101 98-99 1.750062896582996 1101 100-101 0.5715046839715967 1102 1 -0.4878067396157597 1102 2 -0.46026191876399025 1102 3 0.1307269902425361 1102 4 -3.898532803106036E-4 1102 5 -0.8722395422955742 1102 6 -0.023918477935382043 1102 7 0.006746066349812452 1102 8 -0.3580677866176387 1102 9 0.04345743275892744 1102 10-11 0.342320557406687 1102 12-13 0.10424468869398851 1102 14-15 0.5590807378291913 1102 16-17 -0.1657308530182675 1102 18-19 -0.6141389382995683 1102 20-21 0.2790177847245374 1102 22-23 0.38006487516636156 1102 24-25 0.3850773891449464 1102 26-27 0.4310307462642342 1102 28-29 -0.40784038024959557 1102 30-31 -0.4552585067994528 1102 32-33 -0.28394331437598197 1102 34-35 0.15192259456886603 1102 36-37 0.6645076653503068 1102 38-39 0.29581055554906044 1102 40-41 0.29911221754638717 1102 42-43 -0.7616441883407887 1102 44-45 -0.848385776924566 1102 46-47 -0.543998607391849 1102 48-49 -0.30361916747973083 1102 50-51 -0.4614731081427479 1102 52-53 -0.06884782328562267 1102 54-55 -0.42850603759074346 1102 56-57 -0.7078974978055257 1102 58-59 -0.45354497817849193 1102 60-61 -0.002428703482138417 1102 62-63 0.6080726342651062 1102 64-65 -0.15066542976658326 1102 66-67 0.484534846546687 1102 68-69 0.5525500703919626 1102 70-71 0.28813845593093035 1102 72-73 0.5190506870324683 1102 74-75 -0.09687579801281387 1102 76-77 -0.02135430666770688 1102 78-79 0.8468891440512145 1102 80-81 0.8180677153452756 1102 82-83 1.3317836389738282 1102 84-85 1.058651975694353 1102 86-87 1.5968211783584856 1102 88-89 1.949941166704587 1102 90-91 1.6521868158213913 1102 92-93 2.2722742920195955 1102 94-95 2.4102114452709387 1102 96-97 1.6183211506969783 1102 98-99 1.989033484818286 1102 100-101 2.169543899657871 1103 1 0.1516268115824957 1103 2 -0.18466279022368326 1103 3 -0.2625191971212928 1103 4 -0.2269693739774752 1103 5 0.062400980580243015 1103 6 -0.013025231748024169 1103 7 -0.09456112319266907 1103 8 -0.2164555861819082 1103 9 0.29618074430577224 1103 10-11 -0.13589295021858305 1103 12-13 -0.09945901500970677 1103 14-15 0.3597343326004392 1103 16-17 -0.2839225741511626 1103 18-19 -0.0357075657505419 1103 20-21 -0.24549201919703734 1103 22-23 -0.21742967821054293 1103 24-25 0.21786606016890886 1103 26-27 0.35804599680889027 1103 28-29 0.10414219055650165 1103 30-31 0.6591205781678724 1103 32-33 0.3473203021817497 1103 34-35 0.3746894791004607 1103 36-37 0.7396710640431152 1103 38-39 0.5697756971612549 1103 40-41 -0.19653048397866257 1103 42-43 -0.10096880707717304 1103 44-45 0.41305213157216514 1103 46-47 -0.42417289933084845 1103 48-49 -1.14566709776296 1103 50-51 -0.730536288970633 1103 52-53 -1.1265820280786585 1103 54-55 -0.9410332707062139 1103 56-57 -0.42848573309964877 1103 58-59 -0.9606255882002799 1103 60-61 -0.06615419367822284 1103 62-63 -0.45565285593097116 1103 64-65 0.03125178156238917 1103 66-67 -0.2992342166341473 1103 68-69 0.30200540808258935 1103 70-71 0.5985959722707968 1103 72-73 0.4188328221087225 1103 74-75 0.25334206691093186 1103 76-77 0.14803567154580222 1103 78-79 -0.06542022414050663 1103 80-81 0.9471526826655357 1103 82-83 0.5284067326557462 1103 84-85 0.6899155922520848 1103 86-87 1.6970390432822349 1103 88-89 2.211379075201318 1103 90-91 1.612426467237512 1103 92-93 0.9607274510609898 1103 94-95 1.764786608669631 1103 96-97 1.1646174469932724 1103 98-99 1.8033036373237366 1103 100-101 1.8215046839715967 1104 1 -0.32985466989898526 1104 2 0.09311498755409531 1104 3 0.29303635843425724 1104 4 0.19895655194844863 1104 5 0.19203061020986922 1104 6 0.3017895830667925 1104 7 -0.20567223430378334 1104 8 -0.2534926232189463 1104 9 -0.5927081445831206 1104 10-11 -0.24700406132969732 1104 12-13 0.21535579980510278 1104 14-15 -0.18656196369586553 1104 16-17 0.10496631473773022 1104 18-19 0.5198479898050081 1104 20-21 0.09710057339555789 1104 22-23 0.004792544011685607 1104 24-25 -0.06917097686812923 1104 26-27 0.006194144957042624 1104 28-29 0.011549597963906422 1104 30-31 0.1591205781678653 1104 32-33 0.226949931811383 1104 34-35 0.09691170132267501 1104 36-37 0.2674488418208938 1104 38-39 1.106812734198293 1104 40-41 0.5442102567620779 1104 42-43 -0.332450288558654 1104 44-45 0.051941020461057974 1104 46-47 0.298049322891373 1104 48-49 0.26174030964445194 1104 50-51 -1.462017770452114 1104 52-53 -2.117322768819392 1104 54-55 -1.9039962336691758 1104 56-57 -0.8173746219885345 1104 58-59 -0.5532181807928751 1104 60-61 -0.6309690084930324 1104 62-63 -1.1778750781531926 1104 64-65 -0.1261556258450227 1104 66-67 -0.9844194018193306 1104 68-69 -0.5498464437692583 1104 70-71 -1.1236262499514247 1104 72-73 -0.7385745852986858 1104 74-75 -2.2373986738298086 1104 76-77 -1.5741865506764192 1104 78-79 -1.3246794833997662 1104 80-81 -1.0621065765937239 1104 82-83 -0.9438154895664788 1104 84-85 -0.6711955188590224 1104 86-87 -0.6362942900511008 1104 88-89 -0.6960283322060867 1104 90-91 -0.4616476068365607 1104 92-93 -0.696679956346415 1104 94-95 -0.4574356135525903 1104 96-97 -0.011308478932654964 1104 98-99 0.9977480817681794 1104 100-101 -0.8359027234358116 1105 1 -0.12615096619528288 1105 2 -0.05503316059405705 1105 3 -0.13288956749166658 1105 4 0.1434009963928915 1105 5 0.21054912872838827 1105 6 0.19067847195568532 1105 7 0.46099443236288806 1105 8 0.22798885826254178 1105 9 0.2406251887502151 1105 10-11 -0.09885591318154496 1105 12-13 -0.06242197797267579 1105 14-15 -0.8902656673995679 1105 16-17 -0.09873738896597928 1105 18-19 -0.38755941760239665 1105 20-21 -0.13438090808592307 1105 22-23 -0.05076301154387153 1105 24-25 -0.2543561620533126 1105 26-27 -0.30862066985777403 1105 28-29 -0.3680800316657198 1105 30-31 -0.7297683107210275 1105 32-33 -0.4119389570775027 1105 34-35 -0.3382734838625012 1105 36-37 -0.7695881952161514 1105 38-39 -1.2635576361720737 1105 40-41 -0.7983823358305173 1105 42-43 -0.832450288558654 1105 44-45 -0.948058979538942 1105 46-47 -0.12787660303455084 1105 48-49 -0.18270413479999092 1105 50-51 0.3713155628812217 1105 52-53 0.18823278673616528 1105 54-55 0.21637413670119088 1105 56-57 0.4326253780114655 1105 58-59 0.17826330068861296 1105 60-61 0.9431050655810438 1105 62-63 1.0628656625875479 1105 64-65 -0.1631926628820537 1105 66-67 0.9044694870695551 1105 68-69 0.9316350377122227 1105 70-71 0.7467441204189456 1105 72-73 0.34475874803464635 1105 74-75 0.5588976224664854 1105 76-77 0.805443078953207 1105 78-79 1.0179131091928255 1105 80-81 1.5675230530359059 1105 82-83 1.370999325248338 1105 84-85 1.847322999659493 1105 86-87 1.6322242284674182 1105 88-89 1.2576753714976157 1105 90-91 1.6865005413115846 1105 92-93 1.7014681918017303 1105 94-95 2.16293475681778 1105 96-97 2.0998026321784593 1105 98-99 1.8773777113978056 1105 100-101 1.1455787580456729 1106 1 -0.17340511689770466 1106 2 0.017763774271841726 1106 3 0.2476979165440909 1106 4 0.1625580845154957 1106 5 0.12370366257257359 1106 6 0.2353783442417594 1106 7 0.1947109074586706 1106 8 0.3231357292714776 1106 9 0.5350057762342502 1106 10-11 0.3724084546345452 1106 12-13 0.31401480363651757 1106 14-15 0.6547534896885594 1106 16-17 0.534404373486133 1106 18-19 -0.2138684852907744 1106 20-21 0.13381832563054985 1106 22-23 0.22861119024412346 1106 24-25 0.3206757664268878 1106 26-27 0.536845485953215 1106 28-29 0.2532481931107782 1106 30-31 0.24724318353185026 1106 32-33 -0.0821177565666531 1106 34-35 -0.3497677367360694 1106 36-37 0.03756378434962926 1106 38-39 0.20419459882153745 1106 40-41 0.06145163607242665 1106 42-43 -0.02178617617040146 1106 44-45 0.5787609438327053 1106 46-47 0.6042434480510153 1106 48-49 0.3843456736291273 1106 50-51 -0.34484024810217306 1106 52-53 -0.08794856703140397 1106 54-55 -0.02468588884158862 1106 56-57 -0.6123937790766618 1106 58-59 -0.046513199949956174 1106 60-61 -1.0287978718391386 1106 62-63 -0.7401356145516615 1106 64-65 -0.9767303384886965 1106 66-67 -0.7899749573748878 1106 68-69 -1.36529982818816 1106 70-71 -1.0374193533997023 1106 72-73 -0.4888938701007284 1106 74-75 -0.41939101099456266 1106 76-77 -0.18210481376710064 1106 78-79 0.002906723496785446 1106 80-81 0.17320632251228218 1106 82-83 0.1037579459379927 1106 84-85 0.2030062691358694 1106 86-87 0.6995933216985826 1106 88-89 0.15007639320898392 1106 90-91 0.8991442194725074 1106 92-93 0.0063851777532022425 1106 94-95 -0.6212287170008679 1106 96-97 0.03498781736364265 1106 98-99 -0.5207704367503396 1106 100-101 0.2956426150060807 1107 1 -0.5218624671699388 1107 2 -0.30649514889815066 1107 3 -0.38630087353455167 1107 4 0.23696824785488246 1107 5 -0.1539732884256111 1107 6 -0.0909979412996762 1107 7 0.35865525107633545 1107 8 0.208495680874627 1107 9 0.3994945844617135 1107 10-11 0.13798619208160545 1107 12-13 0.19196398693960504 1107 14-15 -0.6168738545340702 1107 16-17 0.28576553501063273 1107 18-19 0.04323980267050587 1107 20-21 0.32029745448717506 1107 22-23 0.23042607227679213 1107 24-25 0.29632609915526587 1107 26-27 0.24011227361200582 1107 28-29 8.283504005532905E-4 1107 30-31 0.6649685313842397 1107 32-33 0.4033631871720118 1107 34-35 -0.04002786982741924 1107 36-37 0.32446638568054453 1107 38-39 0.15700766597217353 1107 40-41 0.42140323921821476 1107 42-43 0.4112144287902808 1107 44-45 0.8048649970692452 1107 46-47 1.4198816815658404 1107 48-49 0.9956584382994222 1107 50-51 1.318196654499154 1107 52-53 0.1989540342995184 1107 54-55 0.46881078387468733 1107 56-57 0.7425668984793035 1107 58-59 0.4453198309030384 1107 60-61 0.26279317474283204 1107 62-63 0.7592594247707822 1107 64-65 0.31682683029533365 1107 66-67 1.0784460952566945 1107 68-69 0.990114569875967 1107 70-71 0.5605842763643629 1107 72-73 0.443199293843616 1107 74-75 -0.7052349311397528 1107 76-77 -0.586369786543866 1107 78-79 -0.7325742202418724 1107 80-81 -0.45489410096019256 1107 82-83 -0.49742172738324086 1107 84-85 0.32490584566339464 1107 86-87 0.12004099259997147 1107 88-89 -0.4143519189507252 1107 90-91 -0.949464370969114 1107 92-93 -0.4934635820774105 1107 94-95 -0.3828742100438163 1107 96-97 -0.49571393702232314 1107 98-99 -1.0558581560485862 1107 100-101 -1.3670918072564717 1108 1 -0.016254184167351582 1108 2 0.25462075561723907 1108 3 0.05776009856783304 1108 4 0.06871975777723094 1108 5 -0.458546196104642 1108 6 -0.1119930520273158 1108 7 -0.1501166787482262 1108 8 -0.19156183997668563 1108 9 0.04602895317037081 1108 10-11 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163.0 12 214.0 13 305.0 14 375.0 15 514.0 16 703.0 17 981.0 18 910.0 19 617.0 20 538.0 21 514.0 22 579.0 23 618.0 24 704.0 25 692.0 26 734.0 27 799.0 28 924.0 29 1082.0 30 1285.0 31 1486.0 32 1833.0 33 2308.0 34 3126.0 35 5451.0 36 6663.0 37 12106.0 38 12225.0 39 1225.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25067024128686 23.85221179624665 16.18800268096515 16.70911528150134 2 14.084154282851896 26.535314743696777 37.780931708131575 21.599599265319753 3 21.472699949908165 30.834863917181497 31.60460844882284 16.087827684087493 4 10.799799632659877 21.132075471698116 40.63449657705794 27.43362831858407 5 9.333778594089164 42.39271998664218 34.975788946401735 13.297712472866923 6 22.90031724828853 41.83336116213057 17.435298046418435 17.831023543162463 7 20.921689764568374 38.73434630155285 24.21439305393221 16.12957087994657 8 22.659876440140255 32.02204040741359 21.8500584404742 23.46802471197195 9 22.54466521956921 22.25580230422441 25.70712973785273 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18.097257940616547 30.337163087405226 34.60138271934805 16.964196252630174 36-37 17.208665297055337 29.966093768268443 36.21870354595714 16.60653738871908 38-39 16.466943356520723 31.584397364487387 35.427435719713735 16.521223559278155 40-41 15.722671743614153 31.952203211503296 35.20796279131908 17.117162253563468 42-43 15.784244144783536 32.16048094184444 33.109840103536094 18.94543480983593 44-45 17.032627571466737 30.310746727224153 33.287135987176065 19.36948971413305 46-47 18.783082035098264 31.768772228622954 33.16301825042996 16.28512748584882 48-49 17.50390302139774 31.10311490328021 33.45160670902245 17.941375366299603 50-51 14.73630603027242 31.855333572662985 35.65316123861445 17.755199158450147 52-53 16.428452162297546 33.36951076974453 32.43028886291535 17.771748205042577 54-55 15.52262481215562 30.943396226415093 33.32275839038237 20.21122057104692 56-57 17.697445316413425 30.710469193521455 31.936884287861076 19.65520120220404 58-59 19.205657415756605 30.053768827438798 32.73803560097519 18.00253815582941 60-61 17.059098575555666 32.07588130187198 33.78671743232637 17.07830269024598 62-63 15.848445379554487 31.908125438332835 33.85265337474535 18.390775807367334 64-65 15.762757146673792 33.48250066791344 32.28777050494256 18.466971680470213 66-67 16.539758875196206 30.988043950171996 31.018101058678155 21.454096115953643 68-69 15.69789770734884 30.880658573647036 32.64063987175848 20.780803847245647 70-71 18.370345633661714 30.467523793621638 31.262314242778427 19.89981632993822 72-73 17.174820504257806 28.700951744865588 34.206879278677576 19.91734847219903 74-75 15.397395224578394 32.96877608949742 31.676406745700454 19.957421940223742 76-77 16.460598962690312 29.714739836038145 32.27789861134348 21.546762589928058 78-79 16.727333444648522 29.858907997996326 33.28351978627484 20.130238771080315 80-81 17.91669101869401 29.66577886132703 31.713853937931553 20.703676182047406 82-83 16.835030889964937 28.72933711804976 33.60160293872099 20.83402905326432 84-85 16.295708799465686 29.508265152780094 32.52295875772249 21.673067290031724 86-87 16.407895176655412 30.165123508373163 31.808837784537218 21.6181435304342 88-89 16.4821720367034 29.32417145011941 30.7923073700088 23.401349143168392 90-91 17.163728972588967 27.667381542792036 31.861973058106024 23.30691642651297 92-93 18.003840374019035 29.149273668392052 31.729838036400064 21.11704792118885 94-95 16.97027884454834 27.947069627650695 32.322591417598936 22.760060110202037 96-97 15.525129403907162 28.308565703790283 33.253464685256304 22.91284020704625 98-99 16.977382970019285 28.81271028661596 30.8181036426025 23.39180310076225 100-101 16.22474536650526 27.369343796961093 32.12138921355819 24.284521622975454 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1048.0 1 623.5 2 219.5 3 231.0 4 190.5 5 194.0 6 307.5 7 521.5 8 736.0 9 760.5 10 552.0 11 365.0 12 325.5 13 301.0 14 274.0 15 244.5 16 215.0 17 207.5 18 202.5 19 205.5 20 212.0 21 232.0 22 260.5 23 281.0 24 302.0 25 332.5 26 403.0 27 464.5 28 498.5 29 599.5 30 746.0 31 869.0 32 1080.5 33 1394.0 34 1699.0 35 1929.0 36 2361.5 37 2688.5 38 2659.5 39 2614.5 40 2409.0 41 2095.5 42 1839.0 43 1730.5 44 1684.0 45 1660.0 46 1718.5 47 2704.0 48 4846.5 49 4672.5 50 2578.0 51 1313.0 52 536.0 53 237.5 54 165.5 55 134.0 56 119.5 57 114.5 58 97.0 59 85.5 60 78.5 61 59.5 62 40.5 63 30.0 64 24.0 65 16.5 66 12.5 67 13.5 68 11.5 69 7.0 70 5.0 71 4.5 72 6.0 73 5.5 74 3.0 75 1.5 76 1.5 77 1.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35064284521622974 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.348639171814994E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.348639171814994E-4 26-27 0.0016697278343629988 28-29 0.0 30-31 0.004174319585907497 32-33 0.0 34-35 0.01335782267490399 36-37 0.03172482885289698 38-39 0.025880781432626485 40-41 0.019201870095174486 42-43 0.012522958757722491 44-45 0.0033394556687259976 46-47 0.0016697278343629988 48-49 8.348639171814994E-4 50-51 8.348639171814994E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.006678911337451995 60-61 0.011688094840540992 62-63 0.006678911337451995 64-65 0.0033394556687259976 66-67 0.006678911337451995 68-69 0.005009183503088996 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.20036734012355986 78-79 0.0 80-81 0.007513775254633495 82-83 0.0 84-85 0.0 86-87 0.09350475872432794 88-89 0.3715144431457672 90-91 0.3439639338787778 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.002504591751544498 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 59890.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.428285189514106 #Duplication Level Percentage of deduplicated Percentage of total 1 72.41493071920068 25.655368174987476 2 11.103779809595625 7.867757555518451 3 4.967480441134885 5.279679412255802 4 2.7382411160335565 3.8804474870596093 5 1.8474879819021586 3.2726665553514778 6 1.3337732114242624 2.8351978627483723 7 1.0698463568668113 2.653197528802805 8 0.7305118295786596 2.0704625146101185 9 0.5419926477519088 1.728168308565704 >10 2.99274201149967 18.76607113040574 >50 0.08954661136770665 2.1005176156286525 >100 0.13196342727872562 11.504424778761061 >500 0.02356489772834386 6.0093504758724325 >1k 0.014138938637006316 6.376690599432293 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1509 2.519619302053765 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1199 2.0020036734012354 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1111 1.8550676239772916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 879 1.467690766405076 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 875 1.4610118550676239 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 627 1.0469193521456004 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 610 1.0185339789614292 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 608 1.0151945232927033 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 439 0.7330105192853564 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 438 0.7313407914509935 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 434 0.7246618801135415 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 404 0.6745700450826515 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 388 0.6478543997328435 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 378 0.6311571213892136 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 341 0.5693771915177827 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 314 0.5242945399899817 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 297 0.49590916680581065 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 285 0.47587243279345465 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 277 0.4625146101185507 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 233 0.3890465854065787 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 228 0.3806979462347637 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 218 0.3640006678911338 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 215 0.35899148438804473 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 210 0.35064284521622974 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 209 0.34897311738186676 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 194 0.32392719986642177 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 173 0.2888629153447988 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 158 0.2638169978293538 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 152 0.25379863082317583 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 146 0.24378026381699786 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 143 0.2387710803139088 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 130 0.21706461846718986 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 127 0.21205543496410087 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 126 0.21038570712973784 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 120 0.20036734012355986 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 113 0.18867924528301888 TruSeq Adapter, Index 1 (95% over 21bp) GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 99 0.16530305560193687 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86 0.14359659375521788 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 75 0.1252295875772249 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 72 0.12022040407413592 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 71 0.11855067623977292 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 70 0.11688094840540991 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 69 0.11521122057104691 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 69 0.11521122057104691 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 69 0.11521122057104691 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 67 0.11187176490232092 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 65 0.10853230923359493 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 64 0.10686258139923192 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.002504591751544498 0.0 22-23 0.0 0.0 0.0 0.007513775254633495 0.0 24-25 0.0 0.0 0.0 0.01335782267490399 0.0 26-27 0.0 0.0 0.0 0.022541325763900485 0.0 28-29 0.0 0.0 0.0 0.06511938554015695 0.0 30-31 0.0 0.0 0.0 0.1410920020036734 0.0 32-33 0.0 0.0 0.0 0.2788445483386208 0.0 34-35 0.0 0.0 0.0 0.4591751544498247 0.0 36-37 0.0 0.0 0.0 0.7246618801135415 0.0 38-39 0.0 0.0 0.0 1.0310569377191519 0.0 40-41 0.0 0.0 0.0 1.334112539656036 0.0 42-43 0.0 0.0 0.0 1.6471865085990984 0.0 44-45 0.0 0.0 0.0 1.9936550342294206 0.0 46-47 0.0 0.0 0.0 2.408582401068626 0.0 48-49 0.0 0.0 0.0 2.81182167306729 0.0 50-51 0.0 0.0 0.0 3.188345299716146 0.0 52-53 0.0 0.0 0.0 3.537318417098013 0.0 54-55 0.0 0.0016697278343629988 0.0 3.9263650025045918 0.0 56-57 0.0 0.0016697278343629988 0.0 4.422274169310402 0.0 58-59 0.0 0.0016697278343629988 0.0 4.882284187677408 0.0 60-61 0.0 0.0016697278343629988 0.0 5.4232760060110206 0.0 62-63 0.0 0.0016697278343629988 0.0 5.918350308899649 0.0 64-65 0.0 0.0016697278343629988 0.0 6.486892636500251 0.0 66-67 0.0 0.0016697278343629988 0.0 7.144765403239272 0.0 68-69 0.0 0.0016697278343629988 0.0 7.85106027717482 0.0 70-71 0.0 0.0016697278343629988 0.0 8.643346134580064 0.0 72-73 0.0 0.0016697278343629988 0.0 9.374686926031057 0.0 74-75 0.0 0.0016697278343629988 0.0 10.158624144264486 0.0 76-77 0.0 0.0016697278343629988 0.0 10.988478877942896 0.0 78-79 0.0 0.0016697278343629988 0.0 12.024544999165137 0.0 80-81 0.0 0.0016697278343629988 0.0 13.082317582234095 0.0 82-83 0.0 0.0016697278343629988 0.0 14.032392719986643 0.0 84-85 0.0 0.0016697278343629988 0.0 15.024211053598263 0.0 86-87 0.0 0.0016697278343629988 0.0 16.000166972783436 0.0 88-89 0.0 0.0016697278343629988 0.0 17.073801970278843 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGCTTA 40 7.4864365E-6 59.33793 6 ATATAGG 60 1.4748512E-6 47.470345 2 CTTACTA 65 1.318033E-4 36.515648 9 GATATAG 70 1.9881135E-4 34.049614 1 TAGGACA 70 2.0377118E-4 33.907387 4 GGACAAT 70 2.0377118E-4 33.907387 6 GACAATG 70 2.0377118E-4 33.907387 7 ATGGGGA 60 0.003933366 31.646896 5 GTAATGA 30 0.003949004 31.646896 62-63 TTACTAG 40 4.4862728E-4 29.668964 10-11 AGGACAA 80 4.4597764E-4 29.668964 5 GGCTTAC 80 4.4597764E-4 29.668964 7 GAGGGTG 40 4.4862728E-4 29.668964 16-17 TATAGGC 80 4.4597764E-4 29.668964 3 ATAGGCT 85 6.3591235E-4 27.92373 4 ATACGTA 45 8.9331286E-4 26.372412 24-25 GGAGGGT 45 8.9331286E-4 26.372412 16-17 AATACGT 45 8.9331286E-4 26.372412 24-25 CATGGGG 90 8.880758E-4 26.372412 4 TTCTAGG 100 0.0016018266 23.834732 1 >>END_MODULE