FastQCFastQC Report
Fri 27 May 2016
SRR939154_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939154_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58839
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13462.287598361630891No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12652.1499345672088244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10661.8117235167151038No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9081.5431941399411955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8361.4208263226771358No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6371.0826152721834157No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6311.0724179540780774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.8990635462873264No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4520.768197963935485No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4140.7036149492683424No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3970.674722547969884No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3500.5948435561447339No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3390.5761484729516138No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3370.572749366916501No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3300.5608524957936063No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3270.5557538367409371No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA2720.4622784207753361No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC2430.41299138326620094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.4095922772310882No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.4027940651608627No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2330.3959958530906371No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG2100.3569061336868404No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC1940.3297132854059382No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT1910.3246146263532691No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1800.30591954316014885No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.28552490694947225No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.2821258009143595No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1650.28042624789680315No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC1590.27022892979146484No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC1530.26003161168612654No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT1380.23453831642278078No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG1300.22094189228232972No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG1260.21414368021210423No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT1250.21244412719454783No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.17165485477319464No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.16145753666785637No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG890.15126021856251806No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT830.14106290045717976No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG720.12236781726405956No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC700.1189687112289468No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA680.11556960519383402No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT650.11047094614116486No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA640.10877139312360849No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.10537228708849572No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA600.10197318105338296No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG590.10027362803582658No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA590.10027362803582658No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAACC200.002105733170.8935247
TGGGAAC250.005097838656.714826
GAATTAG550.002623560134.372627
CATGGGG1001.3048466E-633.1401024
GTATTGG600.003934988331.6430021
TTGGAAT600.00398475331.5620024
TCTAGGA600.00398475331.5620022
AATTAGT753.1341176E-431.5082348
ATGGGGA804.5293424E-429.5893785
GTACATG3550.029.414621
TGAAGAA650.00589410229.1341575
ACATGGG3550.028.005723
GGCATAA350.00856853227.0070614-15
TACATGG3950.026.3682562
CATGGGA2301.3278623E-1024.7006974
GAGTACT5800.024.03860912-13
GTACTTT6350.022.3286714-15
TGGAAGG550.002831954721.63028332-33
ACTTTTT6800.021.54607216-17
TGGAGTT550.002917655721.51954820-21
Produced by FastQC (version 0.11.5)