##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939154_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 58839 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31530107581706 33.0 31.0 34.0 31.0 34.0 2 32.50060334132123 34.0 31.0 34.0 31.0 34.0 3 32.59390880198508 34.0 31.0 34.0 31.0 34.0 4 36.08355002634307 37.0 35.0 37.0 35.0 37.0 5 36.06373323815836 37.0 35.0 37.0 35.0 37.0 6 36.15584901170992 37.0 36.0 37.0 35.0 37.0 7 36.12547799928619 37.0 36.0 37.0 35.0 37.0 8 36.124424276415304 37.0 36.0 37.0 35.0 37.0 9 37.78419075783069 39.0 38.0 39.0 35.0 39.0 10-11 37.72372915923113 39.0 37.5 39.0 35.0 39.0 12-13 37.677467326093236 39.0 37.0 39.0 35.0 39.0 14-15 38.87805707099033 40.0 38.0 41.0 35.5 41.0 16-17 38.98529886639814 40.0 38.0 41.0 36.0 41.0 18-19 39.020581587042614 40.5 38.5 41.0 35.0 41.0 20-21 39.02068356022366 40.0 38.5 41.0 35.0 41.0 22-23 38.908156154931255 40.0 38.0 41.0 35.0 41.0 24-25 38.76305681605738 40.0 38.0 41.0 35.0 41.0 26-27 38.49964309386631 40.0 38.0 41.0 34.5 41.0 28-29 38.33032512449226 40.0 37.0 41.0 34.0 41.0 30-31 38.08789238430293 40.0 37.0 41.0 34.0 41.0 32-33 37.73801390234368 40.0 35.5 41.0 33.5 41.0 34-35 37.499184214551576 40.0 35.0 41.0 33.0 41.0 36-37 37.22780808647326 40.0 35.0 41.0 33.0 41.0 38-39 37.01675759275311 39.5 35.0 41.0 33.0 41.0 40-41 36.856685191794554 40.0 35.0 41.0 33.0 41.0 42-43 36.60240656707286 39.5 35.0 41.0 31.5 41.0 44-45 36.22520777035639 39.0 35.0 41.0 31.0 41.0 46-47 35.7378609425721 39.0 35.0 41.0 29.0 41.0 48-49 35.27325413416271 39.0 35.0 41.0 27.0 41.0 50-51 34.97935043083669 39.0 35.0 40.5 25.0 41.0 52-53 34.793623277078126 39.0 35.0 40.0 24.0 41.0 54-55 34.55939088019851 38.0 35.0 40.0 23.0 41.0 56-57 34.30444093203488 38.0 35.0 40.0 22.5 41.0 58-59 33.99077992487976 38.0 34.5 40.0 20.0 41.0 60-61 33.48374377538707 38.0 34.0 40.0 13.5 41.0 62-63 33.09514947568789 37.0 33.0 40.0 5.0 41.0 64-65 32.576573361206 36.5 33.0 39.5 2.0 41.0 66-67 32.04547154098472 36.0 32.5 39.0 2.0 40.0 68-69 31.409498801815122 35.5 31.5 38.5 2.0 40.0 70-71 30.792909464810755 35.0 31.0 37.5 2.0 40.0 72-73 30.19826135726304 35.0 30.5 37.0 2.0 39.0 74-75 29.62597086966128 35.0 29.5 36.5 2.0 39.0 76-77 27.64041707031051 32.5 27.0 34.5 2.0 36.5 78-79 28.546992640935436 34.0 29.0 35.0 2.0 37.0 80-81 28.456041061200906 34.0 29.0 35.0 2.0 37.0 82-83 28.046202348782273 34.0 28.5 35.0 2.0 36.5 84-85 27.548173830282636 34.0 27.5 35.0 2.0 36.0 86-87 26.97638471082105 33.5 26.0 35.0 2.0 36.0 88-89 26.30169615391152 33.0 24.5 35.0 2.0 35.0 90-91 25.731003246146265 32.5 22.5 35.0 2.0 35.0 92-93 25.353558014242253 32.0 20.0 34.5 2.0 35.0 94-95 24.891814952667445 32.0 18.5 34.0 2.0 35.0 96-97 24.099007461037747 31.5 3.5 34.0 2.0 35.0 98-99 23.085037135233435 31.0 2.0 34.0 2.0 35.0 100-101 17.79896837131834 23.5 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15100372718490718 1101 2 0.20805013297005104 1101 3 -0.09757262504948727 1101 4 -0.11368010239154813 1101 5 -0.07332096609173533 1101 6 -0.2835262052351126 1101 7 0.0792669467898861 1101 8 -0.20121894398978668 1101 9 -0.39482200269729617 1101 10-11 -0.3250875917439586 1101 12-13 0.08449753975312291 1101 14-15 -0.06895549425644987 1101 16-17 -0.1557183490472127 1101 18-19 -0.17329086123400828 1101 20-21 0.3000716145705198 1101 22-23 0.16462433356952744 1101 24-25 -0.31466115438956166 1101 26-27 0.026556805493775926 1101 28-29 -0.8384226266636929 1101 30-31 -0.47802834375022485 1101 32-33 -0.3421667160219428 1101 34-35 -0.21548054260283322 1101 36-37 -0.6026151890538927 1101 38-39 -0.8821020522915077 1101 40-41 0.2064361368747214 1101 42-43 -1.1103976758513667 1101 44-45 0.15868425591344248 1101 46-47 -0.8020051739103948 1101 48-49 -1.206900780656241 1101 50-51 -1.3320279770184626 1101 52-53 -0.6307678884399266 1101 54-55 -0.6350440632990342 1101 56-57 -0.4255072593700717 1101 58-59 -1.124407062496303 1101 60-61 -0.3067242987301455 1101 62-63 -1.9472828934949789 1101 64-65 -2.032736215818936 1101 66-67 -1.943377095222413 1101 68-69 -1.5738795851884966 1101 70-71 -0.9293271090462163 1101 72-73 0.08263256029681187 1101 74-75 -0.617017360923672 1101 76-77 -0.18510225563569804 1101 78-79 0.12085674082816311 1101 80-81 -0.11026472127642961 1101 82-83 0.253288901591457 1101 84-85 -0.39712049222621104 1101 86-87 -0.2439727212017324 1101 88-89 0.767135805638901 1101 90-91 1.8411869595324681 1101 92-93 1.3746104438567066 1101 94-95 0.583985343472964 1101 96-97 0.3289252637660134 1101 98-99 0.2586925910175708 1101 100-101 0.519966232509276 1102 1 0.35554918173036043 1102 2 0.13986831478823092 1102 3 -0.21120898868585414 1102 4 0.1344259582145142 1102 5 -0.550593693364462 1102 6 0.1179889462800432 1102 7 0.3501002801232218 1102 8 0.11506893479809577 1102 9 0.466920421545133 1102 10-11 0.5252911961348232 1102 12-13 0.09017935793494303 1102 14-15 0.2113475360465813 1102 16-17 0.3082968024679289 1102 18-19 -0.17044995214310177 1102 20-21 0.34647312972203537 1102 22-23 -0.016246878551690713 1102 24-25 0.2885585425801267 1102 26-27 0.2973901388271116 1102 28-29 0.7647970703059954 1102 30-31 0.18579741382554005 1102 32-33 0.6180605567053306 1102 34-35 0.5723982452759486 1102 36-37 0.5138620836733807 1102 38-39 0.856534311344852 1102 40-41 0.40814068232926815 1102 42-43 0.8545644453607579 1102 44-45 1.0459948619740516 1102 46-47 0.49155543215021424 1102 48-49 0.3158264920710323 1102 50-51 0.6471386896482088 1102 52-53 0.23570938428734678 1102 54-55 0.5051074518524885 1102 56-57 1.8453260739632569 1102 58-59 1.687145967806721 1102 60-61 1.7377832770274324 1102 62-63 1.4703307428686614 1102 64-65 1.7267334811507595 1102 66-67 2.2204486623533484 1102 68-69 1.6799082935993859 1102 70-71 2.2723774364083305 1102 72-73 1.8866098330240852 1102 74-75 2.208740214833899 1102 76-77 2.3291022898188487 1102 78-79 3.3718037105251355 1102 80-81 2.8632201272084146 1102 82-83 2.5459025379550937 1102 84-85 2.009129507773789 1102 86-87 2.5069742484952364 1102 88-89 3.1392948965479945 1102 90-91 2.9879672625627656 1102 92-93 3.4825649893112534 1102 94-95 3.0801974646850816 1102 96-97 2.5704025364932868 1102 98-99 2.1895638031387854 1102 100-101 2.1497010809941237 1103 1 0.7098272565966752 1103 2 0.693344250617109 1103 3 0.4416430612250224 1103 4 0.18166303486335522 1103 5 0.024718249594542385 1103 6 0.015493402608029783 1103 7 0.45794341737812516 1103 8 0.15294772267688472 1103 9 0.22405054632231725 1103 10-11 0.4212359376678023 1103 12-13 0.28180146132175565 1103 14-15 0.41143666260629885 1103 16-17 0.3038404744821932 1103 18-19 0.5283022760208951 1103 20-21 -0.4382862285667457 1103 22-23 0.23509001984403 1103 24-25 0.37100061031631526 1103 26-27 0.33415484470946666 1103 28-29 0.21978815765002224 1103 30-31 0.17822165624978226 1103 32-33 0.7693528918211925 1103 34-35 1.1399116142599013 1103 36-37 0.868585791338262 1103 38-39 0.28946657515947294 1103 40-41 0.5930782937374701 1103 42-43 0.24315624571726602 1103 44-45 0.3713068049330488 1103 46-47 1.1673575711876438 1103 48-49 0.822510984049643 1103 50-51 0.777040650432518 1103 52-53 0.3992566213639961 1103 54-55 -0.21837739663236988 1103 56-57 -0.09339941623282044 1103 58-59 0.35782332966055463 1103 60-61 -0.0879742987301384 1103 62-63 -0.0018172072204691858 1103 64-65 -0.4628832746424649 1103 66-67 -0.6523231736537838 1103 68-69 -0.6596638989139834 1103 70-71 -0.7632731874775907 1103 72-73 -0.3223919495071108 1103 74-75 0.022688521429270025 1103 76-77 0.28119676397214377 1103 78-79 0.24034203494581519 1103 80-81 0.13483331793925402 1103 82-83 -0.1492846278203075 1103 84-85 -0.6648901000693463 1103 86-87 -0.4357619368880066 1103 88-89 0.3106407075996884 1103 90-91 -0.44986696203616106 1103 92-93 -0.2736738698687837 1103 94-95 0.26290691210041217 1103 96-97 0.2462046755307199 1103 98-99 -0.07647897760987732 1103 100-101 -0.9169210223926854 1104 1 0.22792680410798738 1104 2 0.38112705604697084 1104 3 0.6716581441812792 1104 4 0.29176861555717437 1104 5 -0.03485942763019523 1104 6 -0.06557748728639012 1104 7 0.215484895507835 1104 8 0.3388451585743155 1104 9 0.37601133063603953 1104 10-11 0.16689958774321667 1104 12-13 0.1469975397531229 1104 14-15 0.030403480102528135 1104 16-17 0.18242267659380929 1104 18-19 0.3916129849198313 1104 20-21 0.4531164863653885 1104 22-23 0.5099768976720824 1104 24-25 0.9729991020206867 1104 26-27 0.8935439849809583 1104 28-29 0.7473145528234824 1104 30-31 0.6045037075318334 1104 32-33 0.23956405320882368 1104 34-35 0.6274681753458822 1104 36-37 0.26036558017687383 1104 38-39 0.7044364092469593 1104 40-41 0.7761476753362544 1104 42-43 0.7750189908153047 1104 44-45 0.6290368200160046 1104 46-47 1.0169050824998607 1104 48-49 -0.18767001142546746 1104 50-51 -0.38170746419794455 1104 52-53 -0.4600948115168464 1104 54-55 -0.4940184222733919 1104 56-57 -0.2508277721905898 1104 58-59 -0.41687501121425186 1104 60-61 0.040230829474985796 1104 62-63 0.32435172188963435 1104 64-65 -1.3292105747932936 1104 66-67 -1.3127681208634385 1104 68-69 -1.3270847133936208 1104 70-71 -0.6096155705846762 1104 72-73 -0.5303482089339546 1104 74-75 -1.3830430019493143 1104 76-77 -1.3509676402510848 1104 78-79 -1.3030214643000413 1104 80-81 -0.789751900763612 1104 82-83 -0.6385380214854663 1104 84-85 -0.7312551076108278 1104 86-87 0.21676445828544644 1104 88-89 -0.11507573282263905 1104 90-91 0.19775747235297914 1104 92-93 0.04287967462593656 1104 94-95 0.37565201013962835 1104 96-97 -0.9330939670032201 1104 98-99 -0.702044588469608 1104 100-101 -0.18596325467020947 1105 1 -0.09409431203077645 1105 2 0.026677583950444728 1105 3 -0.20541576230439063 1105 4 -0.014415396509193101 1105 5 -0.5243013582486 1105 6 -0.41587914641158363 1105 7 0.10500224090753818 1105 8 0.015692820716097344 1105 9 -0.4230082772070958 1105 10-11 0.12711829060897628 1105 12-13 -0.11035540142334099 1105 14-15 -0.5493476511191915 1105 16-17 -0.03929678041976814 1105 18-19 -0.12856046907714358 1105 20-21 0.0028902420215004554 1105 22-23 0.34293315709893335 1105 24-25 -0.05056801713466541 1105 26-27 0.03023327608201498 1105 28-29 -0.270407920781345 1105 30-31 0.09979028370076293 1105 32-33 0.28896073495844377 1105 34-35 -0.2914609347596979 1105 36-37 -0.3569044047401704 1105 38-39 -0.21053342484052706 1105 40-41 -0.1520197454782206 1105 42-43 0.3117836966976597 1105 44-45 -0.30516378330224114 1105 46-47 0.2850046300111728 1105 48-49 0.3323149056182686 1105 50-51 0.1495896700403634 1105 52-53 0.7031781899914407 1105 54-55 0.0855441719950889 1105 56-57 0.16150254455148882 1105 58-59 -0.3676668664178706 1105 60-61 0.42182962283848724 1105 62-63 0.7923004398383569 1105 64-65 0.8018226077104771 1105 66-67 1.2790493753658225 1105 68-69 1.2324929638311097 1105 70-71 1.8739817144831967 1105 72-73 1.1481962857870087 1105 74-75 1.3952375410371083 1105 76-77 1.5753144110309698 1105 78-79 2.014851838867383 1105 80-81 2.1740490042137637 1105 82-83 1.1056173329640053 1105 84-85 0.7566785273816343 1105 86-87 0.9858066905629741 1105 88-89 -0.6109279198512922 1105 90-91 0.3834663712971711 1105 92-93 2.030247698758668 1105 94-95 2.5864363238651187 1105 96-97 0.6187536951385617 1105 98-99 0.6097955321940418 1105 100-101 -1.1031955321966045 1106 1 0.21240723595683875 1106 2 0.24752381718057848 1106 3 0.17435719951191686 1106 4 0.04750410813476691 1106 5 0.295100086539847 1106 6 -0.059841994708797586 1106 7 -0.2025312988241481 1106 8 0.13540386302776142 1106 9 -0.24240972199554278 1106 10-11 0.03785100474726022 1106 12-13 -0.017476144457397425 1106 14-15 0.11525503205934484 1106 16-17 0.14800972112821853 1106 18-19 0.39414334929230677 1106 20-21 -0.10288891174527492 1106 22-23 -0.014651982219952231 1106 24-25 0.17492217894376694 1106 26-27 -0.11269758047113498 1106 28-29 -0.18217262666369294 1106 30-31 -0.2516029051537316 1106 32-33 0.43085959976752974 1106 34-35 0.027940510028741983 1106 36-37 0.776222530244354 1106 38-39 0.48193303542779375 1106 40-41 0.1302299965238447 1106 42-43 0.4351067101135513 1106 44-45 0.6767763611766 1106 46-47 0.3175123699492559 1106 48-49 -0.6542692017088712 1106 50-51 -1.0480367489482845 1106 52-53 -0.9975442042293992 1106 54-55 -0.025394940492013518 1106 56-57 -0.12177918919463337 1106 58-59 -1.566841273022618 1106 60-61 -0.5616585092564605 1106 62-63 -0.8305065777055063 1106 64-65 -0.7662888473978811 1106 66-67 -0.6358113057487245 1106 68-69 -0.5848444974691951 1106 70-71 -0.06255079325674373 1106 72-73 0.3024790515248803 1106 74-75 0.19657913030439644 1106 76-77 -1.2492469924778042 1106 78-79 -1.4970160661893779 1106 80-81 -0.7857033177676627 1106 82-83 -1.0729172387594197 1106 84-85 -0.4842915448577898 1106 86-87 -1.2500034229561194 1106 88-89 -0.7301229662909243 1106 90-91 -0.5014665492394634 1106 92-93 0.16079465438302165 1106 94-95 -0.2690848319656318 1106 96-97 -1.09486859588311 1106 98-99 -0.044487233543829774 1106 100-101 1.0314793904040123 1107 1 -0.43990536372418276 1107 2 -0.2692225943026756 1107 3 -0.3793908068676686 1107 4 -0.2928467690582153 1107 5 -0.45513914790991805 1107 6 -1.928719017740832E-4 1107 7 0.0682820983050405 1107 8 -0.1258401561109963 1107 9 -0.14671594209123384 1107 10-11 -0.08607244022881133 1107 12-13 0.11745208520766681 1107 14-15 0.03407480877385893 1107 16-17 0.1537513479224799 1107 18-19 0.06591368422053279 1107 20-21 0.09647312972203537 1107 22-23 0.5110258487210402 1107 24-25 0.14764945167103605 1107 26-27 0.2951174115543864 1107 28-29 0.5329788884878113 1107 30-31 0.05397923200735022 1107 32-33 0.26578782943260393 1107 34-35 0.2746709725486767 1107 36-37 1.0138620836733807 1107 38-39 0.23153431134485203 1107 40-41 -0.36913204494346274 1107 42-43 -0.8908901000937917 1107 44-45 -0.09036877438958868 1107 46-47 0.28019179578657827 1107 48-49 0.3476446738892136 1107 50-51 -0.04149767398815385 1107 52-53 -0.2870178884399266 1107 54-55 -0.36080163905661067 1107 56-57 0.24759880123598776 1107 58-59 0.8462368768976347 1107 60-61 0.16051054975470436 1107 62-63 0.16805801559592481 1107 64-65 -0.48463015521286934 1107 66-67 -0.15909679219210915 1107 68-69 0.06172647541756504 1107 70-71 -0.16398619995530694 1107 72-73 -0.5542992578850061 1107 74-75 -0.6298961488024624 1107 76-77 -0.4186249829084261 1107 78-79 -0.3963781076566839 1107 80-81 -0.5163253273370358 1107 82-83 -0.254097462044907 1107 84-85 -0.534052310408029 1107 86-87 -0.4134802969593103 1107 88-89 -1.0084323761792788 1107 90-91 -0.10976001016450354 1107 92-93 0.6598377165839793 1107 94-95 -0.5107116262240083 1107 96-97 0.24994799103874143 1107 98-99 0.4941092576842401 1107 100-101 0.0837919900850359 1108 1 0.2760037271849072 1108 2 0.10090727582719694 1108 3 0.15242737495051273 1108 4 0.44286751665607227 1108 5 0.42667903390826467 1108 6 0.12123569952679247 1108 7 -0.2391854341624935 1108 8 -0.01967132494216628 1108 9 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2316 50-51 -1.6940775265680088 2316 52-53 -2.712693564115604 2316 54-55 -2.4841431623981336 2316 56-57 -2.171565817928631 2316 58-59 -2.2305444498836913 2316 60-61 -2.0699562807121232 2316 62-63 -2.3586004610625473 2316 64-65 -1.74163261221533 2316 66-67 -1.8062712393665556 2316 68-69 -0.9128435491524591 2316 70-71 -0.8136176495867566 2316 72-73 -1.1901714937572407 2316 74-75 -0.9739993429056568 2316 76-77 -0.8210819853654279 2316 78-79 -1.4807761420547187 2316 80-81 -1.3490034600151724 2316 82-83 -1.1039746119220588 2316 84-85 -1.8456002219559409 2316 86-87 -2.267902901381916 2316 88-89 -2.6576952754421796 2316 90-91 -2.563936914341408 2316 92-93 -2.6034300966838337 2316 94-95 -2.3338074493198313 2316 96-97 -2.415162574071825 2316 98-99 -1.8018366882617052 2316 100-101 -0.4746846683916246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 4.0 6 14.0 7 20.0 8 37.0 9 53.0 10 93.0 11 144.0 12 242.0 13 335.0 14 374.0 15 496.0 16 615.0 17 956.0 18 907.0 19 625.0 20 545.0 21 542.0 22 668.0 23 652.0 24 731.0 25 788.0 26 810.0 27 873.0 28 1051.0 29 1184.0 30 1377.0 31 1607.0 32 1997.0 33 2577.0 34 3708.0 35 5915.0 36 7617.0 37 12474.0 38 8418.0 39 389.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.23101611581535 23.601816443594647 16.334334881180006 16.832832559409997 2 13.980523122418804 26.786655109706146 37.7572698380326 21.475551929842453 3 21.465354611737116 31.134111728615377 31.470623226091536 15.929910433555975 4 11.011404000747802 20.997977531909108 40.663505498054015 27.327112969289075 5 9.383232209928789 42.497323203997354 34.956406465099676 13.163038120974182 6 23.035741599959213 41.58296368055201 17.901391933921378 17.479902785567393 7 21.293699756963917 38.45918523428338 24.016383691089242 16.230731317663455 8 22.5139788235694 32.32209928788728 21.982018729074255 23.18190315946906 9 22.581960944271657 22.269243189041283 25.476299733170176 29.672496133516884 10-11 22.035554649127278 29.593466918200512 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17.26421188630491 29.864511083911328 36.01761185910512 16.85366517067863 38-39 16.47895823947912 31.507815753907874 35.33536766768383 16.67785833892917 40-41 15.893775228383259 31.803696622052264 35.34820480135968 16.9543233482048 42-43 15.785403494459175 32.095995648922425 33.10048269766809 19.018118158950305 44-45 16.79045498580825 30.225878273875285 33.458537994799194 19.525128745517275 46-47 18.76662389421892 31.795508043203004 33.05148839620317 16.38637966637491 48-49 17.740386658168685 30.917357127682177 33.398767792649245 17.943488421499893 50-51 14.881286221723686 31.60573769098727 35.73990040619317 17.77307568109587 52-53 16.376892877173304 33.39536701847414 32.40962626829144 17.818113836061116 54-55 15.445537823552405 30.959057767807067 33.177824232226925 20.4175801764136 56-57 17.71784020802529 30.860483692788797 31.702612212987987 19.71906388619793 58-59 19.159910943421878 30.071041316133858 32.69770050476725 18.071347235677017 60-61 16.88010401352856 32.00540462452306 33.75255997348675 17.361931388461638 62-63 15.873960077160362 31.69863269797836 33.794199376258746 18.63320784860253 64-65 15.565488583155588 33.42199410250091 32.46683718993516 18.545680124408335 66-67 16.600525166345165 30.999260688154866 31.10463387067991 21.295580274820058 68-69 15.555291178395098 31.009458092915352 32.40565276137223 21.02959796731732 70-71 18.3058855520998 30.598752528085114 31.296419041792007 19.79894287802308 72-73 17.24706402216217 28.528696952701438 34.14741922874284 20.07681979639355 74-75 15.64948418565917 32.571083804959294 31.673719811689526 20.105712197692007 76-77 16.766889794251984 29.527689651359605 32.25965586751835 21.44576468687006 78-79 16.72984090290998 29.745716616807176 33.05429018221376 20.470152298069078 80-81 17.69829534832339 29.641904179200868 31.585343054776594 21.074457417699143 82-83 17.050765648634407 28.5924301908598 33.40981321912337 20.946990941382417 84-85 16.2757694726287 29.508489267322695 32.75208620132905 21.463655058719556 86-87 16.573387316276538 29.99200462710942 31.789262384322264 21.64534567229178 88-89 16.38445068115707 29.391693039999318 30.866609228249466 23.357247050594147 90-91 16.96421717064017 27.515673646948436 31.935855333304904 23.584253849106496 92-93 18.105338296028144 29.38612145005863 31.308315912914903 21.200224340998318 94-95 17.058413637213413 27.989938646136064 32.2787606859396 22.67288703071092 96-97 15.506721732184436 28.4445690783324 33.06055507401553 22.988154115467633 98-99 16.718503033702138 28.89070174544095 30.706674144699942 23.684121076156973 100-101 16.29361477931304 27.073029793164398 31.904859022077193 24.72849640544537 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1002.0 1 594.0 2 209.5 3 223.0 4 181.5 5 170.0 6 294.5 7 505.5 8 651.0 9 693.5 10 557.5 11 407.0 12 361.5 13 294.5 14 240.5 15 227.5 16 233.5 17 227.5 18 223.5 19 209.0 20 212.5 21 239.5 22 258.5 23 287.0 24 304.5 25 320.5 26 395.5 27 477.0 28 523.5 29 598.5 30 725.5 31 868.0 32 1044.5 33 1333.5 34 1618.5 35 1892.0 36 2307.0 37 2610.5 38 2626.0 39 2497.0 40 2329.0 41 2111.0 42 1813.0 43 1685.0 44 1638.5 45 1641.5 46 1784.0 47 2697.0 48 4680.5 49 4509.5 50 2576.0 51 1419.0 52 615.0 53 250.5 54 149.0 55 120.5 56 118.0 57 113.5 58 89.5 59 76.5 60 66.0 61 48.5 62 33.0 63 28.0 64 21.5 65 15.0 66 18.5 67 14.5 68 6.5 69 4.5 70 3.0 71 3.5 72 4.0 73 3.5 74 1.5 75 2.0 76 3.0 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.44698244361732864 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.497765087781913E-4 16-17 8.497765087781913E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0016995530175563827 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011896871122894679 36-37 0.02549329526334574 38-39 0.02634307177212393 40-41 0.002549329526334574 42-43 0.005098659052669148 44-45 0.0033991060351127654 46-47 8.497765087781913E-4 48-49 0.002549329526334574 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 8.497765087781913E-4 62-63 8.497765087781913E-4 64-65 8.497765087781913E-4 66-67 8.497765087781913E-4 68-69 8.497765087781913E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09092608643926647 78-79 0.011896871122894679 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.09347541596560105 88-89 0.3832492054589643 90-91 0.3764509933887388 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 58839.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.01352844201975 #Duplication Level Percentage of deduplicated Percentage of total 1 72.69466729589429 26.179914682438522 2 11.297781972628599 8.137459848059962 3 4.837187352524776 5.226125528985877 4 2.638036809815951 3.800200547256072 5 1.7555450684285039 3.1611686126548717 6 1.3072203869749883 2.824657115178708 7 0.9863142991977347 2.4864460646849875 8 0.6890042472864559 1.985077924505855 9 0.580462482302973 1.8814051904349156 >10 2.930627654554035 18.0135624330801 >50 0.10854176498348278 2.5068407008956646 >100 0.1368570080226522 11.499175716786485 >500 0.023596035865974516 5.953534220500009 >1k 0.014157621519584709 6.3444314145379765 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1474 2.505141147878108 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1220 2.073454681418787 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1039 1.7658355852410816 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 838 1.4242254287122487 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 816 1.3868352623260083 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 634 1.0775166131307465 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 627 1.065619742007852 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 588 0.9993371743231529 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 430 0.7308077975492445 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 429 0.7291082445316881 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 407 0.6917180781454477 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 391 0.6645252298645457 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 374 0.6356328285660872 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 370 0.6288346164958616 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 322 0.5472560716531553 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 278 0.4724757388806744 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 270 0.45887931474022337 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 268 0.4554802087051105 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 260 0.44188378456465943 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 217 0.36880300480973505 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 216 0.3671034517921787 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 214 0.3637043457570659 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 214 0.3637043457570659 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 208 0.3535070276517276 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 207 0.3518074746341712 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 188 0.31951596730059995 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 180 0.30591954316014885 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 157 0.2668298237563521 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 150 0.2549329526334574 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 148 0.25153384659834466 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 142 0.24133652849300632 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 129 0.21924233926477335 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 125 0.21244412719454783 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 124 0.21074457417699144 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 121 0.2056459151243223 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 115 0.195448597018984 TruSeq Adapter, Index 1 (95% over 21bp) GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 112 0.19034993796631486 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 92 0.15635887761518721 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 82 0.13936334743962336 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 82 0.13936334743962336 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81 0.137663794422067 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 76 0.1291660293342851 RNA PCR Primer, Index 1 (95% over 23bp) GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 71 0.12066826424650316 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 70 0.1189687112289468 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 69 0.11726915821139039 No Hit GGGTTATTCTATGGCCAATGCTCTGAAATTTGTGGATCTAACCATAGCTT 64 0.10877139312360849 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 63 0.10707184010605211 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 61 0.10367273407093935 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 60 0.10197318105338296 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 60 0.10197318105338296 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 59 0.10027362803582658 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.497765087781913E-4 0.0 12-13 0.0 0.0 0.0 0.0016995530175563827 0.0 14-15 0.0 0.0 0.0 0.0016995530175563827 0.0 16-17 0.0 0.0 0.0 0.0016995530175563827 0.0 18-19 0.0 0.0 0.0 0.0016995530175563827 0.0 20-21 0.0 0.0 0.0 0.0016995530175563827 0.0 22-23 0.0 0.0 0.0 0.008497765087781913 0.0 24-25 0.0 0.0 0.0 0.014446200649229253 0.0 26-27 0.0 0.0 0.0 0.021244412719454785 0.0 28-29 0.0 0.0 0.0 0.06373323815836435 0.0 30-31 0.0 0.0 0.0 0.1478611125274053 0.0 32-33 0.0 0.0 0.0 0.29912133108992334 0.0 34-35 0.0 0.0 0.0 0.5158143408283621 0.0 36-37 0.0 0.0 0.0 0.8225836604972891 0.0 38-39 0.0 0.0 0.0 1.1539964989207838 0.0 40-41 0.0 0.0 0.0 1.4726626897126056 0.0 42-43 0.0 0.0 0.0 1.7870799979605363 0.0 44-45 0.0 0.0 0.0 2.1550332262614935 0.0 46-47 0.0 0.0 0.0 2.6445044953177312 0.0 48-49 0.0 0.0 0.0 3.051547443022485 0.0 50-51 0.0 0.0 0.0 3.443294413569231 0.0 52-53 0.0 0.0 0.0 3.8477880317476503 0.0 54-55 0.0 0.0 0.0 4.303268240452761 0.0 56-57 0.0 0.0 0.0 4.888764255000935 0.0 58-59 0.0 0.0 0.0 5.381634630092286 0.0 60-61 0.0 0.0 0.0 5.908496065534765 0.0 62-63 0.0 0.0 0.0 6.4090144292051185 0.0 64-65 0.0 0.0 0.0 6.97241625452506 0.0 66-67 0.0 0.0 0.0 7.648838355512501 0.0 68-69 0.0 0.0 0.0 8.37709682353541 0.0 70-71 0.0 0.0 0.0 9.173337412260576 0.0 72-73 0.0 0.0 0.0 9.902445656792263 0.0 74-75 0.0 0.0 0.0 10.740325294447562 0.0 76-77 0.0 0.0 0.0 11.462635326909023 0.0 78-79 0.0 0.0 0.0 12.50191199714475 0.0 80-81 0.0 0.0 0.0 13.52164380767858 0.0 82-83 0.0 0.0 0.0 14.431754448580023 0.0 84-85 0.0 0.0 0.0 15.447237376569962 0.0 86-87 0.0 0.0 0.0 16.476316728700354 0.0 88-89 0.0 0.0 0.0 17.54788490626965 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGGGA 20 0.0020713978 71.18813 6 ATGGGGG 25 0.005014888 56.950512 5 CATGGGC 50 2.8085225E-5 47.45876 4 GTAAGCG 20 5.391392E-4 47.458755 94-95 CATGGGG 105 4.5651177E-8 36.159054 4 ACATGGG 490 0.0 25.182196 3 GTACATG 500 0.0 24.847586 1 TACATGG 530 0.0 23.281656 2 AGTACTT 580 0.0 21.683743 12-13 AGAACAT 55 0.0028133057 21.655027 82-83 CTCCCCC 55 0.002848361 21.608913 72-73 TACTTTT 660 0.0 19.05541 14-15 GAGTACT 575 0.0 18.983503 12-13 GTACTTT 590 0.0 18.500872 14-15 CATGGGA 325 9.569703E-9 17.523233 4 ACTTTTT 675 0.0 16.522678 16-17 AGAGTAC 1135 0.0 15.052999 10-11 CTTTTTT 925 0.0 14.109361 16-17 AGTACAT 385 1.2534256E-6 13.559645 2 CTTGAAA 160 4.6800786E-5 13.347775 56-57 >>END_MODULE