##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939153_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 268537 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14134737484965 33.0 31.0 34.0 31.0 34.0 2 32.221165053605276 34.0 31.0 34.0 30.0 34.0 3 32.24096120832511 34.0 31.0 34.0 30.0 34.0 4 35.68268804671237 37.0 35.0 37.0 35.0 37.0 5 35.755419178735146 37.0 35.0 37.0 35.0 37.0 6 35.879394645802996 37.0 37.0 37.0 35.0 37.0 7 35.84170896375546 37.0 36.0 37.0 35.0 37.0 8 35.8560198408413 37.0 37.0 37.0 35.0 37.0 9 37.49647162216007 39.0 38.0 39.0 35.0 39.0 10-11 37.43806998663126 39.0 38.0 39.0 35.0 39.0 12-13 37.390149215936724 39.0 38.0 39.0 35.0 39.0 14-15 38.59410062672927 40.0 38.0 41.0 34.5 41.0 16-17 38.566128689901205 40.0 38.0 41.0 34.5 41.0 18-19 38.64312552832571 40.0 38.5 41.0 35.0 41.0 20-21 38.60117600181725 40.0 38.0 41.0 35.0 41.0 22-23 38.522879528705545 40.0 38.0 41.0 34.0 41.0 24-25 38.50947355485464 40.0 38.0 41.0 34.5 41.0 26-27 38.36365752205469 40.0 38.0 41.0 34.0 41.0 28-29 38.23379832201894 40.0 38.0 41.0 34.0 41.0 30-31 38.03042597481911 40.0 37.5 41.0 34.0 41.0 32-33 37.74366288444423 40.0 37.0 41.0 34.0 41.0 34-35 37.4023412043778 40.0 36.0 41.0 33.0 41.0 36-37 37.07504738639368 40.0 35.5 41.0 33.0 41.0 38-39 36.793210991409005 39.5 35.0 41.0 31.5 41.0 40-41 36.662407787381255 40.0 35.0 41.0 31.0 41.0 42-43 36.41350726343111 40.0 35.0 41.0 30.5 41.0 44-45 36.036847808681856 40.0 35.0 41.0 30.0 41.0 46-47 35.49478842766546 39.0 35.0 41.0 27.0 41.0 48-49 35.207997408178386 39.5 35.0 41.0 24.0 41.0 50-51 34.159086084971534 38.5 34.0 40.0 22.0 40.5 52-53 34.334670827483734 38.5 35.0 40.0 22.0 40.5 54-55 34.87225224084577 39.5 35.0 41.0 21.5 41.0 56-57 34.81163303380912 39.5 35.0 41.0 19.5 41.0 58-59 34.66239289185475 39.0 35.0 41.0 16.5 41.0 60-61 34.35356952673189 39.0 35.0 41.0 10.5 41.0 62-63 34.01386028740919 39.0 35.0 40.5 7.5 41.0 64-65 33.62510566514112 38.0 34.5 40.0 2.0 41.0 66-67 33.18257819220443 37.5 34.0 40.0 2.0 41.0 68-69 32.72381459538164 37.0 34.0 39.0 2.0 41.0 70-71 32.233589784647926 36.0 33.0 39.0 2.0 41.0 72-73 31.712291043692304 36.0 33.0 38.5 2.0 40.0 74-75 31.221477859661796 35.0 32.0 37.0 2.0 39.5 76-77 30.725929760144783 35.0 31.5 37.0 2.0 39.0 78-79 30.25764605994705 35.0 31.0 36.5 2.0 39.0 80-81 29.79732774254572 35.0 31.0 36.0 2.0 37.5 82-83 29.318058591553495 35.0 30.5 35.5 2.0 37.0 84-85 28.919191768732055 35.0 30.0 35.0 2.0 37.0 86-87 28.5651102082767 34.0 29.5 35.0 2.0 36.0 88-89 28.103615144281793 34.0 29.0 35.0 2.0 36.0 90-91 27.688761697643155 34.0 28.5 35.0 2.0 36.0 92-93 27.2875618629835 34.0 27.0 35.0 2.0 35.0 94-95 26.819077073177997 34.0 25.5 35.0 2.0 35.0 96-97 26.281938801729368 34.0 24.5 35.0 2.0 35.0 98-99 25.69191210149812 34.0 21.0 35.0 2.0 35.0 100-101 23.454764147957263 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31680134066102283 1101 2 0.4449397416588141 1101 3 0.49523445805733246 1101 4 0.34949077734548695 1101 5 0.4300998336721591 1101 6 0.540023803172943 1101 7 0.37640787113799945 1101 8 0.44623239025246875 1101 9 0.4512703475946225 1101 10-11 0.409351451425799 1101 12-13 0.21367885128794484 1101 14-15 0.4756244613477847 1101 16-17 0.46990018837321657 1101 18-19 0.5964700896440576 1101 20-21 0.32513942162100307 1101 22-23 0.436157780863212 1101 24-25 0.38828084917789596 1101 26-27 0.3355238503913469 1101 28-29 0.2633076208795373 1101 30-31 0.6230152185044844 1101 32-33 0.3201512338928083 1101 34-35 -0.09404964353582557 1101 36-37 0.48455478331543844 1101 38-39 0.31237802175028406 1101 40-41 0.4759544903829678 1101 42-43 0.11235940594960425 1101 44-45 0.3874763737950033 1101 46-47 0.08122459606430255 1101 48-49 0.20131342452073397 1101 50-51 0.2734266667889216 1101 52-53 0.40139715851989166 1101 54-55 0.012645313133099023 1101 56-57 0.2550030229411959 1101 58-59 0.3213818504355572 1101 60-61 -0.1830994905784138 1101 62-63 -0.09845871943473838 1101 64-65 -0.048010920099841314 1101 66-67 0.11071641889846262 1101 68-69 0.4655713202549876 1101 70-71 0.05082196398461036 1101 72-73 -0.029533318135246844 1101 74-75 -0.4573205151371873 1101 76-77 -0.13940309553838404 1101 78-79 -0.43917977699135236 1101 80-81 -0.24849175312297334 1101 82-83 0.1589234122885621 1101 84-85 -0.3755836778060768 1101 86-87 -0.4854210006394908 1101 88-89 -0.36825579995454305 1101 90-91 -0.3521649487914722 1101 92-93 -0.3958020210531963 1101 94-95 -0.09427593850833205 1101 96-97 -0.32890468668988504 1101 98-99 -0.06091629652844688 1101 100-101 -0.7262101457506773 1102 1 0.18909571295539251 1102 2 0.22286181958089202 1102 3 0.38700934983222623 1102 4 0.36117908903379714 1102 5 0.34958035315267466 1102 6 0.2958679590170945 1102 7 0.24437323910336772 1102 8 0.2908211348412095 1102 9 0.254300650624927 1102 10-11 0.25307439514874375 1102 12-13 0.1762329638420539 1102 14-15 0.30311363883696174 1102 16-17 0.40583092430394885 1102 18-19 0.5884614316353947 1102 20-21 0.334879681361258 1102 22-23 0.5339932786987092 1102 24-25 0.552783013680056 1102 26-27 0.722320387187878 1102 28-29 0.788848746420669 1102 30-31 0.6591624046516742 1102 32-33 0.8257789395205108 1102 34-35 0.9524871530009733 1102 36-37 1.010528809289461 1102 38-39 0.8158412252134895 1102 40-41 0.9233570877855612 1102 42-43 0.7837879773781751 1102 44-45 0.6573897937084183 1102 46-47 0.8197527345924414 1102 48-49 1.3720926452999507 1102 50-51 1.4439894373516893 1102 52-53 1.4407910979138236 1102 54-55 1.3076669581547335 1102 56-57 1.3976437155818928 1102 58-59 1.4224641015178037 1102 60-61 1.506943799464878 1102 62-63 1.3104157394397262 1102 64-65 1.251772629683714 1102 66-67 1.4639631721452133 1102 68-69 1.54652370120737 1102 70-71 1.6423804055430509 1102 72-73 1.5990381104361795 1102 74-75 1.5084803506636781 1102 76-77 1.1753155191802307 1102 78-79 1.2632011753896037 1102 80-81 1.3305125758813539 1102 82-83 1.360438563803715 1102 84-85 1.3499574477350471 1102 86-87 1.354189388970898 1102 88-89 1.3129130312142863 1102 90-91 1.2971857005591758 1102 92-93 1.5366655114143342 1102 94-95 1.400962156729765 1102 96-97 1.7334329756477764 1102 98-99 1.250772015159864 1102 100-101 1.0343959148553843 1103 1 0.11764879828814401 1103 2 0.10196153342636904 1103 3 -0.06328047333894915 1103 4 0.25368771035597604 1103 5 0.23223865530250265 1103 6 0.27037085724880683 1103 7 0.31789293974171784 1103 8 0.17698299557934405 1103 9 0.48779173581093005 1103 10-11 0.3393151318132084 1103 12-13 0.39842461399980067 1103 14-15 0.4654146146972664 1103 16-17 0.35028759757418015 1103 18-19 0.24536032370377825 1103 20-21 0.2861967258986482 1103 22-23 0.35778812791728143 1103 24-25 0.4756496950213105 1103 26-27 0.4245270788013542 1103 28-29 0.6442398242693699 1103 30-31 0.534556784283339 1103 32-33 0.5496306527790011 1103 34-35 0.5936016881833126 1103 36-37 0.6409308930813751 1103 38-39 0.35652652861065803 1103 40-41 0.40494964776795683 1103 42-43 0.01663704921029563 1103 44-45 -0.024831939360772992 1103 46-47 0.4770276630538177 1103 48-49 0.8906193163689977 1103 50-51 0.7729020501626138 1103 52-53 0.6776481673980115 1103 54-55 0.7861319959418083 1103 56-57 0.781879536258387 1103 58-59 0.6527176050763899 1103 60-61 0.7758189920688849 1103 62-63 0.48229188589213834 1103 64-65 0.4926468684393015 1103 66-67 0.32613207668700284 1103 68-69 0.20522023066661177 1103 70-71 0.29716982516298174 1103 72-73 -0.043617256795453585 1103 74-75 0.0329135445883999 1103 76-77 -0.30138856769334765 1103 78-79 0.06990012615634811 1103 80-81 -0.0659856998542061 1103 82-83 -0.728526143805059 1103 84-85 -0.5269961071846083 1103 86-87 0.019663745123221332 1103 88-89 -0.07273521884074086 1103 90-91 -0.14897689390850388 1103 92-93 -0.36888515261090404 1103 94-95 -0.006120167725441661 1103 96-97 -0.02807740662531799 1103 98-99 -0.4956418816777628 1103 100-101 -0.511480525088853 1104 1 0.34671587057555087 1104 2 0.42149651821559075 1104 3 0.22905619187906723 1104 4 0.026902254756961952 1104 5 0.07002657359889497 1104 6 0.09240475555389338 1104 7 -0.04361654888641908 1104 8 0.07919942321949947 1104 9 0.2955926919169656 1104 10-11 0.44885084092518923 1104 12-13 0.30726859487768365 1104 14-15 0.48765132337464934 1104 16-17 0.4546987231717452 1104 18-19 0.3795592227331852 1104 20-21 0.39687324335481833 1104 22-23 0.3807853754908095 1104 24-25 0.3971331080301539 1104 26-27 0.7416899065573972 1104 28-29 0.5842477918197133 1104 30-31 0.4547002001894711 1104 32-33 0.4714943352359029 1104 34-35 0.6541188546326779 1104 36-37 0.9509772497379103 1104 38-39 0.9331960925683518 1104 40-41 0.7182011326296092 1104 42-43 0.5811017746919731 1104 44-45 0.6001137364323554 1104 46-47 0.9196250844647977 1104 48-49 1.1076626308699389 1104 50-51 0.9908869842492365 1104 52-53 0.8618977690204943 1104 54-55 0.7386526391404189 1104 56-57 0.699813767751948 1104 58-59 0.6280363070900066 1104 60-61 0.6491349416560226 1104 62-63 0.8802348092587877 1104 64-65 0.8915495194605967 1104 66-67 0.779702987885031 1104 68-69 0.818196472880139 1104 70-71 1.1672444304070808 1104 72-73 1.088354349752425 1104 74-75 0.6442057363890683 1104 76-77 1.0868484307131432 1104 78-79 1.2628959250843508 1104 80-81 1.3461358415046227 1104 82-83 0.8301077834729362 1104 84-85 0.9915103392879381 1104 86-87 1.0244080591895681 1104 88-89 1.0492045175057747 1104 90-91 1.1267727801462577 1104 92-93 1.0856998104486344 1104 94-95 1.2080439638115728 1104 96-97 1.025613017827819 1104 98-99 0.9589249733128185 1104 100-101 0.7356946161540812 1105 1 -6.589767992934981E-4 1105 2 -0.06934597262689834 1105 3 0.13015509297797223 1105 4 -0.010033032178327517 1105 5 -0.0667255631532413 1105 6 -0.09410318095404335 1105 7 -0.16724292251279138 1105 8 -0.39424380022371963 1105 9 -0.056666160341883653 1105 10-11 -0.0577120406376892 1105 12-13 -0.07917829156919964 1105 14-15 0.10181493753826487 1105 16-17 0.0627573312303582 1105 18-19 -0.09876800559403875 1105 20-21 -0.13914629266471934 1105 22-23 -0.04320729850186922 1105 24-25 -0.08790962701258564 1105 26-27 -0.22320630833880983 1105 28-29 -0.019232061660140687 1105 30-31 -0.00515938467011523 1105 32-33 -0.1159598772183088 1105 34-35 0.2670614675752887 1105 36-37 -0.24560394684329623 1105 38-39 -0.12571721634495248 1105 40-41 -0.28317249374402564 1105 42-43 -0.37097392738373003 1105 44-45 -0.4311744198557932 1105 46-47 -0.36837857853886646 1105 48-49 -0.011384988177681521 1105 50-51 0.30437904774130686 1105 52-53 0.33869874582147474 1105 54-55 0.14280404329182517 1105 56-57 -0.0192033262651492 1105 58-59 -0.12425307019937293 1105 60-61 0.05618622370730009 1105 62-63 0.1868587408827196 1105 64-65 0.07103669894777909 1105 66-67 -0.20991850173646043 1105 68-69 -0.36419058450692177 1105 70-71 -0.7297335915709411 1105 72-73 -0.6450095086114338 1105 74-75 -0.4069236897403563 1105 76-77 -0.39376817490346383 1105 78-79 -0.6455289833405544 1105 80-81 -1.0024600070912264 1105 82-83 -0.8724257940606428 1105 84-85 -1.0672503444727433 1105 86-87 -1.1901829054013966 1105 88-89 -1.0202399269386717 1105 90-91 -0.7212125678390926 1105 92-93 -0.9739766242277987 1105 94-95 -0.9899108591432544 1105 96-97 -1.2483491311343293 1105 98-99 -0.8982178838300356 1105 100-101 -1.2623212568617923 1106 1 0.16203943589911773 1106 2 0.06954291626198739 1106 3 0.05475826758114266 1106 4 -0.04177906392435915 1106 5 -0.08656683299451373 1106 6 0.12415078729992501 1106 7 0.1343443790745127 1106 8 0.26051810453818547 1106 9 0.427460823785097 1106 10-11 0.27958954666389246 1106 12-13 0.24026615287524322 1106 14-15 0.32205303277635977 1106 16-17 0.5072017756748011 1106 18-19 0.3694859626599225 1106 20-21 0.4243457708273439 1106 22-23 0.34766571737115015 1106 24-25 0.5331221190191613 1106 26-27 0.445444485311981 1106 28-29 0.3835457161176379 1106 30-31 0.28650728199654907 1106 32-33 0.25705599579756466 1106 34-35 0.6519821024959214 1106 36-37 0.4230468468075017 1106 38-39 0.7512669106391741 1106 40-41 0.48865290308138043 1106 42-43 0.6687086122988077 1106 44-45 0.31485732617595374 1106 46-47 1.1177325325722478 1106 48-49 1.1175832657905715 1106 50-51 1.0504107937730467 1106 52-53 0.703778110900835 1106 54-55 0.5773278528156354 1106 56-57 0.5680982610364396 1106 58-59 0.5106675647212668 1106 60-61 0.5045989221200031 1106 62-63 0.5241603281843084 1106 64-65 0.364687492598577 1106 66-67 0.5043672125492549 1106 68-69 0.48104751073118024 1106 70-71 0.4964568846195334 1106 72-73 0.2895143009123693 1106 74-75 -0.04779670561337568 1106 76-77 -0.5624189685542582 1106 78-79 -0.5344178722294473 1106 80-81 -0.6929361975674162 1106 82-83 -0.5946480162828642 1106 84-85 -0.5454249476473443 1106 86-87 -0.26557973079821906 1106 88-89 -0.0996050063037508 1106 90-91 -0.2708157424422666 1106 92-93 -0.12278614803732424 1106 94-95 -0.07324419247658298 1106 96-97 -0.17493643272162984 1106 98-99 0.15336941775726842 1106 100-101 0.5928374732969424 1107 1 0.18359670745638823 1107 2 0.07544167216074271 1107 3 0.17348413630701032 1107 4 0.2296709575256628 1107 5 0.08750009107241397 1107 6 0.158041821190956 1107 7 0.2921092868394197 1107 8 0.12924397326405312 1107 9 0.16619956252383616 1107 10-11 0.08734372941807322 1107 12-13 0.23549352310261185 1107 14-15 0.30387413959746823 1107 16-17 0.3783943968674208 1107 18-19 0.012235855409819862 1107 20-21 0.08414843063000887 1107 22-23 0.27232251702795196 1107 24-25 0.3348702957673382 1107 26-27 0.07419823906573697 1107 28-29 -0.04518658761466554 1107 30-31 0.2831825286717944 1107 32-33 0.30161841535998946 1107 34-35 0.2389619394757574 1107 36-37 0.4427272414878942 1107 38-39 0.3755697849420443 1107 40-41 0.4784641428926122 1107 42-43 0.4009050944952932 1107 44-45 0.17505681137543405 1107 46-47 0.24042665526636853 1107 48-49 0.3469376201449279 1107 50-51 -0.19658620322394427 1107 52-53 0.11079226791499508 1107 54-55 0.01785766834544944 1107 56-57 0.20886402680220328 1107 58-59 0.2672634463171448 1107 60-61 0.2793200718411555 1107 62-63 0.07547950450348395 1107 64-65 -0.33919496128388005 1107 66-67 -0.17409696591492718 1107 68-69 -0.16261400793033687 1107 70-71 -0.019898756736111523 1107 72-73 0.13829164968972307 1107 74-75 0.11500895719228765 1107 76-77 0.10692895079366238 1107 78-79 0.19112910331752886 1107 80-81 -0.027449282080500126 1107 82-83 0.3311885345536858 1107 84-85 0.3125759103535124 1107 86-87 0.6035751383566499 1107 88-89 0.2500839683852263 1107 90-91 0.6278865312600068 1107 92-93 0.6193845941334182 1107 94-95 0.6693019250695293 1107 96-97 0.6090618512766532 1107 98-99 0.6849009492887959 1107 100-101 0.49952987998934617 1108 1 0.200522270893579 1108 2 0.16985668518041308 1108 3 0.19069403723784717 1108 4 0.07053175187483163 1108 5 0.36221500532221285 1108 6 0.36759855400350716 1108 7 0.34318527977587365 1108 8 0.28432762834770386 1108 9 0.3665527396212056 1108 10-11 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scores pass #Quality Count 2 1418.0 3 218.0 4 224.0 5 200.0 6 205.0 7 264.0 8 288.0 9 358.0 10 446.0 11 680.0 12 1116.0 13 1923.0 14 1768.0 15 2157.0 16 2970.0 17 4653.0 18 4084.0 19 1906.0 20 1687.0 21 1613.0 22 1710.0 23 1905.0 24 2093.0 25 2261.0 26 2564.0 27 2925.0 28 3483.0 29 4036.0 30 4845.0 31 5586.0 32 6999.0 33 8957.0 34 12533.0 35 20252.0 36 28986.0 37 53109.0 38 65513.0 39 12602.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.160970433391995 23.20677943302956 15.53881621474183 18.093433918836617 2 13.89964844521822 24.688987723542986 39.91582071078934 21.495543120449454 3 19.72646348373244 29.94736528196105 33.34513762562252 16.981033608683983 4 10.550450164967286 19.88144723842899 42.72596790128059 26.842134695323132 5 9.57338125741046 41.74465514798547 36.22381637720822 12.458147217395844 6 22.846782518605753 42.3104300285841 18.96128289314317 15.881504559666979 7 22.148884151522832 38.875057767706735 23.388094634684474 15.58796344608596 8 22.844864536472095 33.23282432790038 21.542968531611884 22.37934260401564 9 24.316608964301555 20.50340810416985 25.54568797492649 29.63429495660211 10-11 21.72768283888712 28.412536032989166 29.12918956287116 20.730591565252553 12-13 25.914289867063207 28.51022834419711 28.080895040678584 17.494586748061106 14-15 22.753013653448022 29.009710402298744 30.53754744792142 17.69972849633181 16-17 16.62278617811193 32.296971221480014 31.74376030632494 19.336482294083122 18-19 16.08108561397493 32.51903588772647 34.76253126059879 16.637347237699814 20-21 18.099357767730346 29.90387016695057 33.20337106711943 18.793400998199665 22-23 17.507166207557226 29.722521461347213 33.15764165607422 19.61267067502134 24-25 18.61175216144775 30.485081443425806 33.73871741051289 17.164448984613546 26-27 18.629508807903814 28.505545717215025 36.186224252027216 16.678721222853948 28-29 16.324997576814965 30.672676165552005 35.92017655698959 17.08214970064345 30-31 17.054983067652973 30.411874449023106 34.787073639401584 17.74606884392234 32-33 17.231386245082273 30.89274831851414 34.324489031438056 17.55137640496553 34-35 18.470743836235723 29.393281599683174 34.03343134580968 18.102543218271418 36-37 18.951886787157086 28.663596711992472 34.96006002690733 17.42445647394312 38-39 18.304547656771728 30.084009405461824 34.41093338171996 17.20050955604649 40-41 16.832614558280625 30.57119239357703 35.69625177200181 16.899941276140535 42-43 16.36511942468116 31.159486718014822 33.659107874152745 18.81628598315127 44-45 17.4558687334537 30.38850482239913 33.08175159265461 19.07387485149256 46-47 18.78507313549855 31.059150217338832 31.690677165295394 18.465099481867224 48-49 18.235851139881053 30.417476055199362 32.52488050992413 18.82179229499545 50-51 17.243979567015323 31.044851524053662 33.646501880508204 18.064667028422807 52-53 17.31271226878491 32.34606712137798 32.42409782842601 17.917122781411102 54-55 16.5929820294302 30.727896947528933 32.81420432753024 19.864916695510633 56-57 18.403096019243502 31.733636101769104 31.00003366864813 18.863234210339268 58-59 18.89672041934947 30.093897328199308 31.79268906164385 19.216693190807373 60-61 17.953664345023796 30.569419364968986 32.888258319220284 18.58865797078693 62-63 18.633226635037577 30.062353960215443 32.55875733589759 18.7456620688494 64-65 18.002114659455614 31.211437874860813 32.47892358219569 18.307523883487878 66-67 17.61889789715603 30.37408014612857 31.394434837289737 20.612587119425665 68-69 16.8624294552023 30.702031877357346 32.28616620026767 20.149372467172686 70-71 18.35715970217975 30.96230080743877 31.19027030669197 19.490269183689513 72-73 18.236703222667217 28.771381517385937 32.52853988097466 20.46337537897219 74-75 17.14657897544259 31.694051458078714 31.46272031203502 19.696649254443674 76-77 18.229839117108824 29.561061774689108 31.920602665289486 20.28849644291258 78-79 17.706369159857534 29.458112597647478 32.58769566909102 20.24782257340397 80-81 18.140509395665553 29.884296283824863 31.670454864326402 20.304739456183178 82-83 17.324948734451944 29.878833690071698 32.362967564250326 20.433250011226033 84-85 17.308336373756937 29.16262056448972 31.759610077367086 21.769432984386256 86-87 17.578116229465255 29.430827377165734 32.12345170826629 20.86760468510272 88-89 18.406682859826866 28.3514414781935 31.75499923275859 21.486876429221045 90-91 17.594443446231246 27.559481028958167 32.96162369358848 21.884451831222112 92-93 18.273096127501255 28.967009275132316 32.50975049220335 20.25014410516308 94-95 17.80219163212023 28.222457210769132 32.167207868165185 21.808143288945452 96-97 17.33184351686525 27.44444070118699 32.092069160424785 23.13164662152298 98-99 17.980158663320474 27.64200254894365 31.231858426953675 23.1459803607822 100-101 18.184556945445017 26.633608712330304 32.39953968357924 22.782294658645437 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1800.0 1 1078.5 2 421.5 3 451.5 4 370.0 5 383.0 6 638.5 7 1206.0 8 1788.0 9 1824.5 10 1351.5 11 966.5 12 869.0 13 891.0 14 902.0 15 859.5 16 823.0 17 830.5 18 831.0 19 858.0 20 964.5 21 1123.0 22 1260.5 23 1313.5 24 1412.5 25 1833.0 26 2813.5 27 3769.5 28 3933.5 29 4104.5 30 4858.5 31 5749.0 32 6784.5 33 8337.5 34 10584.5 35 11845.0 36 12668.0 37 14182.0 38 13564.5 39 11590.5 40 10302.5 41 9415.0 42 8513.0 43 8007.0 44 7889.5 45 7412.0 46 7462.5 47 9434.5 48 19514.5 49 19433.5 50 8299.0 51 4001.0 52 1859.0 53 1181.0 54 839.5 55 581.0 56 439.5 57 349.0 58 317.0 59 303.5 60 291.0 61 280.5 62 232.5 63 182.0 64 143.0 65 117.0 66 106.0 67 98.0 68 85.5 69 78.5 70 65.0 71 51.5 72 50.5 73 47.0 74 42.0 75 44.0 76 37.0 77 24.0 78 20.0 79 15.5 80 9.0 81 7.5 82 5.0 83 4.5 84 4.5 85 2.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33663889892268106 2 0.1117164487575269 3 0.10166196836934947 4 0.11246122508257708 5 0.12475003444590504 6 0.07671196148016847 7 0.08229778391804482 8 0.08788360635592116 9 0.07671196148016847 10-11 0.07689815556143102 12-13 0.07969106678036919 14-15 0.08062203718668191 16-17 0.07242949761112993 18-19 0.08490450105572044 20-21 0.09458659328137277 22-23 0.09831047490662366 24-25 0.11692988303287814 26-27 0.11618510670782797 28-29 0.10985450794490145 30-31 0.09812428082536112 32-33 0.09216607022495969 34-35 0.3291911356721793 36-37 0.4928557331019562 38-39 0.4638094564249992 40-41 0.4409075844297061 42-43 0.47535348946327693 44-45 0.4820564763887285 46-47 0.4943452857520565 48-49 0.506447901034122 50-51 0.49229715085816855 52-53 0.4969520028897321 54-55 0.4820564763887285 56-57 0.45654788725575995 58-59 0.4358803442356175 60-61 0.3924971233014445 62-63 0.47386393681317657 64-65 0.504399766140234 66-67 0.5120337234719983 68-69 0.5286049967043648 70-71 0.5198538748850252 72-73 0.5090546181717975 74-75 0.5166885755035618 76-77 0.4930419271832187 78-79 0.465112814993837 80-81 0.46902289070035036 82-83 0.48447699944514167 84-85 0.48578035801397945 86-87 0.4867113284202922 88-89 0.5012344667587707 90-91 0.51873671039745 92-93 0.5105441708218978 94-95 0.5155714110159866 96-97 0.5176195459098746 98-99 0.5092408122530601 100-101 0.49509006207710665 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 268537.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.365867645799277 #Duplication Level Percentage of deduplicated Percentage of total 1 72.64686195135945 16.974569612381163 2 9.745641156408377 4.554307227681846 3 4.209033245147101 2.950431411686285 4 2.409715360341695 2.2522036069517424 5 1.6160392694355017 1.8880079840022046 6 1.1634207758263475 1.6310601518598926 7 0.8526439932425972 1.3945936686564608 8 0.7139897363975393 1.3346391744899213 9 0.6088037484461161 1.2802705027612582 >10 5.015459152774679 24.261461176672118 >50 0.6199598380773277 10.185188633223726 >100 0.344245051477385 15.108160141805413 >500 0.031874541803461574 5.428674633290757 >1k 0.022312179262423102 10.756432074537214 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3628 1.3510242536410253 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3548 1.321233200639018 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2764 1.0292808812193477 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2687 1.000606992704916 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2301 0.8568651619702312 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2153 0.8017517139165179 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1978 0.7365837854746273 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1973 0.7347218446620019 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1846 0.6874285480213155 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1675 0.6237501722295252 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1220 0.4543135582806094 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1093 0.40702026163992294 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1018 0.3790911494505413 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1001 0.37276055068761477 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 980 0.3649403992745878 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 974 0.36270607029943736 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 954 0.3552583070489356 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 886 0.32993591199722944 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 880 0.3277015830220789 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 866 0.32248814874672765 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 849 0.3161575499838011 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 816 0.3038687406204732 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 802 0.2986553063451219 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 683 0.25434111500463624 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 665 0.24763812807918462 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 663 0.24689335175413443 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 651 0.24242469380383339 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 645 0.24019036482868283 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 582 0.21672991058960217 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 575 0.21412319345192654 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 548 0.20406871306374916 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 540 0.20108960776354842 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 512 0.1906627392128459 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 507 0.18880079840022046 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 495 0.18433214044991938 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 490 0.1824701996372939 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 464 0.1727881074116416 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 450 0.16757467313629035 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 449 0.16720228497376527 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 448 0.16682989681124016 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 426 0.1586373572356882 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 417 0.15528586377296238 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 399 0.1485828768475108 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 398 0.14821048868498568 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 397 0.14783810052246057 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 396 0.1474657123599355 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 393 0.14634854787236024 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 370 0.13778362013428316 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 370 0.13778362013428316 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 352 0.13108063320883154 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 341 0.12698436342105557 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 341 0.12698436342105557 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 335 0.12475003444590504 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 335 0.12475003444590504 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 333 0.12400525812085485 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 331 0.12326048179580468 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 330 0.1228880936332796 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 330 0.1228880936332796 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 329 0.12251570547075448 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 327 0.12177092914570431 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 326 0.12139854098317923 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 321 0.11953660017055379 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 307 0.11432316589520253 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 300 0.1117164487575269 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 296 0.11022689610742653 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 289 0.10762017896975092 No Hit TATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCT 277 0.10315152101944983 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 276 0.10277913285692475 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 274 0.10203435653187458 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 272 0.10128958020682438 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 272 0.10128958020682438 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 271 0.1009171920442993 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 3.7238816252508966E-4 0.0 7 0.0 0.0 0.0 3.7238816252508966E-4 0.0 8 0.0 0.0 0.0 3.7238816252508966E-4 0.0 9 0.0 0.0 0.0 3.7238816252508966E-4 0.0 10-11 0.0 0.0 0.0 3.7238816252508966E-4 0.0 12-13 0.0 0.0 0.0 3.7238816252508966E-4 0.0 14-15 0.0 0.0 0.0 3.7238816252508966E-4 0.0 16-17 0.0 0.0 0.0 3.7238816252508966E-4 0.0 18-19 0.0 0.0 0.0 3.7238816252508966E-4 0.0 20-21 0.0 0.0 0.0 9.309704063127241E-4 0.0 22-23 0.0 0.0 0.0 0.0026067171376756276 0.0 24-25 0.0 0.0 0.0 0.005027240194088711 0.0 26-27 0.0 0.0 0.0 0.010240674469439964 0.0 28-29 0.0 0.0 0.0 0.02811530627064427 0.0 30-31 0.0 0.0 0.0 0.07149852720481721 0.0 32-33 0.0 0.0 0.0 0.13499070891534498 0.0 34-35 0.0 0.0 0.0 0.23609409504090684 0.0 36-37 0.0 0.0 0.0 0.3835598074008424 0.0 38-39 0.0 0.0 0.0 0.5887456849521668 0.0 40-41 0.0 0.0 0.0 0.8149714936861587 0.0 42-43 0.0 0.0 0.0 1.0242536410252592 0.0 44-45 0.0 0.0 0.0 1.2711469927793937 0.0 46-47 0.0 0.0 0.0 1.5800429735939554 0.0 48-49 0.0 0.0 0.0 1.8891251484897797 0.0 50-51 0.0 0.0 0.0 2.2205506131371093 0.0 52-53 0.0 0.0 0.0 2.5113857680692044 0.0 54-55 0.0 0.0 0.0 2.816371673177253 0.0 56-57 0.0 0.0 0.0 3.2146408129978363 0.0 58-59 0.0 0.0 0.0 3.646238693364415 0.0 60-61 0.0 0.0 0.0 4.1713060025247914 0.0 62-63 0.0 0.0 0.0 4.650010985450795 0.0 64-65 0.0 0.0 0.0 5.0970629745621645 0.0 66-67 0.0 0.0 0.0 5.624923194941479 0.0 68-69 0.0 0.0 0.0 6.203055817261681 0.0 70-71 0.0 0.0 0.0 6.8806160789760815 0.0 72-73 0.0 0.0 0.0 7.572699479028961 0.0 74-75 0.0 0.0 0.0 8.29047766229607 0.0 76-77 0.0 0.0 0.0 8.978092404398648 0.0 78-79 0.0 0.0 0.0 9.841660553294332 0.0 80-81 0.0 0.0 0.0 10.801118654040225 0.0 82-83 0.0 0.0 0.0 11.684050987387213 0.0 84-85 0.0 0.0 0.0 12.647977746083408 0.0 86-87 0.0 0.0 0.0 13.66087354815165 0.0 88-89 0.0 0.0 0.0 14.643792103136626 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACATAT 70 7.953349E-8 47.45214 1 GTAGATA 55 0.0026103072 34.45268 2 GGGATTC 55 0.0026174842 34.4334 7 CATATAG 100 1.3217505E-6 33.160706 3 GGGAACT 100 1.3275458E-6 33.142147 7 ATAGTGC 30 0.003930619 31.696981 52-53 TAGTGCT 30 0.0039360295 31.68808 54-55 TTAGGAC 60 0.003997874 31.581623 2 TAGATAT 60 0.003997874 31.581623 3 ACATATA 105 1.9346626E-6 31.581623 2 TAGGCAT 110 2.7808273E-6 30.146093 5 ATACTCA 80 4.5706925E-4 29.591206 6 CATTCCG 135 4.3259388E-7 28.056845 9 GGCATTC 135 4.3259388E-7 28.056845 7 GTTAGGA 70 0.008421573 27.115509 1 AGTCCCA 70 0.008514158 27.054813 6 CTTTAGG 125 7.518669E-6 26.528563 2 TGGGATA 90 9.1005774E-4 26.303291 8 TTAGGCA 145 8.0361133E-7 26.136517 4 CCTTTAG 130 1.0061996E-5 25.551153 1 >>END_MODULE