##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939153_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 268537 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.36652304896532 33.0 31.0 34.0 31.0 34.0 2 32.571101933811725 34.0 31.0 34.0 31.0 34.0 3 32.6396883855856 34.0 31.0 34.0 31.0 34.0 4 36.12918517746158 37.0 35.0 37.0 35.0 37.0 5 36.10160611014497 37.0 35.0 37.0 35.0 37.0 6 36.17645985469414 37.0 36.0 37.0 35.0 37.0 7 36.14103829267475 37.0 36.0 37.0 35.0 37.0 8 36.156228005824154 37.0 36.0 37.0 35.0 37.0 9 37.822329883777655 39.0 38.0 39.0 35.0 39.0 10-11 37.77822795368981 39.0 38.0 39.0 35.0 39.0 12-13 37.73182838863918 39.0 37.5 39.0 35.0 39.0 14-15 38.91907632840167 40.0 38.0 41.0 35.5 41.0 16-17 38.88851629384405 40.0 38.0 41.0 35.5 41.0 18-19 39.04253045204199 40.5 38.5 41.0 35.0 41.0 20-21 39.14237330423741 41.0 39.0 41.0 35.0 41.0 22-23 39.09285312638482 41.0 39.0 41.0 35.0 41.0 24-25 38.99603034218748 40.5 38.0 41.0 35.0 41.0 26-27 38.764823841779716 40.0 38.0 41.0 35.0 41.0 28-29 38.60225406554777 40.0 38.0 41.0 34.5 41.0 30-31 38.36464993650782 40.0 37.5 41.0 34.0 41.0 32-33 38.09496456726633 40.0 37.0 41.0 34.0 41.0 34-35 37.95081124761206 40.0 37.0 41.0 34.0 41.0 36-37 37.749075546386536 40.0 36.0 41.0 33.0 41.0 38-39 37.53023419491541 40.0 36.0 41.0 33.0 41.0 40-41 37.35771420698079 40.0 36.0 41.0 33.0 41.0 42-43 37.1260329116658 40.0 36.0 41.0 33.0 41.0 44-45 36.78476150400131 40.0 36.0 41.0 31.5 41.0 46-47 36.268584962221226 40.0 35.0 41.0 30.5 41.0 48-49 35.77299590000633 40.0 35.0 41.0 27.5 41.0 50-51 35.499521481211154 40.0 35.0 41.0 27.0 41.0 52-53 35.263466486927314 39.0 35.0 41.0 26.0 41.0 54-55 35.06885270931008 39.0 35.0 41.0 24.0 41.0 56-57 34.84961662638668 39.0 35.0 41.0 23.0 41.0 58-59 34.60501159989126 39.0 35.0 40.0 22.5 41.0 60-61 34.20469991099923 38.5 34.5 40.0 21.0 41.0 62-63 33.82971247909971 38.0 34.0 40.0 18.5 41.0 64-65 33.373873991293564 37.5 34.0 40.0 13.5 41.0 66-67 32.96381318030663 37.0 33.0 39.0 8.0 41.0 68-69 32.469413898271 36.0 33.0 39.0 4.5 40.0 70-71 32.01260161541985 36.0 32.5 38.5 2.0 40.0 72-73 31.51132432402239 35.0 32.0 37.5 2.0 40.0 74-75 30.985309286988382 35.0 31.0 37.0 2.0 39.0 76-77 28.971629607838025 33.5 28.5 35.5 2.0 37.5 78-79 29.866385265345187 34.0 30.5 36.0 2.0 38.0 80-81 29.855159624185866 35.0 31.0 36.0 2.0 37.0 82-83 29.54224557509766 34.0 30.5 35.0 2.0 37.0 84-85 29.166839206515306 34.0 30.0 35.0 2.0 36.5 86-87 28.683015748295393 34.0 29.5 35.0 2.0 36.0 88-89 28.114552184615157 34.0 29.0 35.0 2.0 36.0 90-91 27.6375210864797 34.0 27.5 35.0 2.0 35.0 92-93 27.409096325646 34.0 27.0 35.0 2.0 35.0 94-95 27.115516669956094 34.0 27.0 35.0 2.0 35.0 96-97 26.565467700912723 33.0 25.5 35.0 2.0 35.0 98-99 25.907826482011792 33.0 24.5 35.0 2.0 35.0 100-101 22.647737555718578 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -5.576233698718625E-4 1101 2 0.10219167919008498 1101 3 0.09174031649663306 1101 4 0.0926159507248343 1101 5 0.04401970197929472 1101 6 0.29428785797384904 1101 7 0.10502117399011013 1101 8 0.040394239856944125 1101 9 0.10242701429228163 1101 10-11 0.21834515595457304 1101 12-13 0.2385526743607116 1101 14-15 0.2531143601828276 1101 16-17 0.2533913222841804 1101 18-19 0.2946291559092771 1101 20-21 0.24980290257641258 1101 22-23 0.17214297630383868 1101 24-25 0.066787219053289 1101 26-27 0.051968625052182915 1101 28-29 0.13266756187606177 1101 30-31 0.5192282775017318 1101 32-33 0.32970170258425924 1101 34-35 0.3015812008154697 1101 36-37 0.4915510272071444 1101 38-39 0.23215762067981416 1101 40-41 0.2699756915437703 1101 42-43 0.22577830394232024 1101 44-45 0.04879585647599782 1101 46-47 0.06547213588543599 1101 48-49 0.2095315892124745 1101 50-51 0.02493372516041603 1101 52-53 0.2009616499879172 1101 54-55 0.1925193026568408 1101 56-57 -0.005337895978421159 1101 58-59 0.10533578717164005 1101 60-61 0.14035221786604524 1101 62-63 -0.16491364681392184 1101 64-65 -0.11037165794158454 1101 66-67 0.2955014429410099 1101 68-69 0.10542450064858144 1101 70-71 0.38787547913157283 1101 72-73 0.013330807883157547 1101 74-75 0.23122180997669517 1101 76-77 -0.040311329565419385 1101 78-79 0.5234748360683206 1101 80-81 0.35761159335726234 1101 82-83 0.6741374794172863 1101 84-85 0.5165062489958956 1101 86-87 0.586159623199972 1101 88-89 0.6102045425495923 1101 90-91 0.8036077074790988 1101 92-93 0.5783077658148272 1101 94-95 0.6530356705653659 1101 96-97 0.3272526459844336 1101 98-99 -0.14658887263870923 1101 100-101 -0.24836121206169892 1102 1 0.20636878355653465 1102 2 0.1506765276749391 1102 3 0.19477061952693475 1102 4 0.1791960373049193 1102 5 0.2020283599879562 1102 6 0.012036775722762627 1102 7 0.19289996186889624 1102 8 0.09710419656690306 1102 9 0.2080547199199856 1102 10-11 0.14042307803249088 1102 12-13 0.43530592111395805 1102 14-15 0.2674000744685472 1102 16-17 0.42720084609370446 1102 18-19 0.19701010829023602 1102 20-21 0.18616653894004997 1102 22-23 0.5396754438363089 1102 24-25 0.5791248813909462 1102 26-27 0.1943928674764237 1102 28-29 0.2729273021358054 1102 30-31 0.4274533857268423 1102 32-33 0.6872774601600184 1102 34-35 0.9130530622873252 1102 36-37 0.969906005562116 1102 38-39 0.5583480968702972 1102 40-41 0.9500622716303511 1102 42-43 0.6833540615180738 1102 44-45 1.0464149040950446 1102 46-47 0.9775933480066428 1102 48-49 1.0761982558791416 1102 50-51 1.3461458463725435 1102 52-53 1.1000958491221198 1102 54-55 1.3412206013581383 1102 56-57 1.3494240087834797 1102 58-59 1.5821756140114687 1102 60-61 1.5784474559612889 1102 62-63 1.4640906821904096 1102 64-65 1.203048255478329 1102 66-67 1.6706096680492308 1102 68-69 1.5924374876615701 1102 70-71 1.5768365180926054 1102 72-73 1.569174963727317 1102 74-75 1.3747283034831952 1102 76-77 1.2577406184865296 1102 78-79 0.883215095808584 1102 80-81 1.0431094288550966 1102 82-83 1.3908041460839513 1102 84-85 1.3279781104677575 1102 86-87 1.0065059435462906 1102 88-89 0.9950530273980789 1102 90-91 1.2265514304228198 1102 92-93 0.9670523545594172 1102 94-95 0.9015205190502122 1102 96-97 0.9201097888415788 1102 98-99 1.100380824330987 1102 100-101 0.37501541131492644 1103 1 0.05388144039122267 1103 2 0.003361977818016726 1103 3 0.015226999305347988 1103 4 0.024012238053323642 1103 5 0.04684456073636056 1103 6 -0.017697614181116705 1103 7 0.10054175510391161 1103 8 0.24773887262530536 1103 9 0.019779718085665365 1103 10-11 0.06691254901348742 1103 12-13 0.21498528130179295 1103 14-15 0.3259150058648359 1103 16-17 0.020824736327760718 1103 18-19 -0.03361943166134296 1103 20-21 0.1528093593964286 1103 22-23 0.09514539761133989 1103 24-25 -0.05466152993783169 1103 26-27 -0.2145971538017335 1103 28-29 0.06041171038292248 1103 30-31 0.031899786783412765 1103 32-33 -0.06596012146739128 1103 34-35 -0.049003948498494765 1103 36-37 0.16719670920875984 1103 38-39 0.3476943438436564 1103 40-41 0.5503631007447396 1103 42-43 -0.042845586291733184 1103 44-45 0.12258520272296636 1103 46-47 -0.0908636187553995 1103 48-49 0.052792283320627575 1103 50-51 0.18659635631457405 1103 52-53 0.15176875248993582 1103 54-55 -0.22896980145937817 1103 56-57 0.03142562298848617 1103 58-59 -0.42636316359511284 1103 60-61 -0.022339468978181287 1103 62-63 -0.45964730298825884 1103 64-65 -0.22489950297790529 1103 66-67 0.13355632591114386 1103 68-69 0.07596525932493137 1103 70-71 -0.3168460705052354 1103 72-73 -0.19734312270602672 1103 74-75 -0.35929473562459435 1103 76-77 -0.3398270680642064 1103 78-79 -0.18998359815282484 1103 80-81 0.06475445050011785 1103 82-83 0.1408041460839513 1103 84-85 0.3205215839434352 1103 86-87 0.1728424319166777 1103 88-89 0.06074933673845351 1103 90-91 0.23958430150653953 1103 92-93 0.5561729796969921 1103 94-95 -0.023437291500819413 1103 96-97 0.11411705276409734 1103 98-99 0.17722065117081343 1103 100-101 -0.17572198688009877 1104 1 0.18161575880350966 1104 2 0.2948656718640805 1104 3 0.025439950196265215 1104 4 0.09383695194583197 1104 5 0.16795132591092 1104 6 0.26583852952451537 1104 7 0.2951310640999978 1104 8 0.17885577831847854 1104 9 0.1855771974424627 1104 10-11 0.1792731168825341 1104 12-13 0.1934366792447122 1104 14-15 0.3177663748348465 1104 16-17 0.30906897796183586 1104 18-19 0.2895009507810684 1104 20-21 0.22623757901109087 1104 22-23 0.5736692278300879 1104 24-25 0.42380797607403764 1104 26-27 0.3054484785320284 1104 28-29 0.41911444832295075 1104 30-31 0.34792180619525936 1104 32-33 0.16138668426924596 1104 34-35 0.22832112755539669 1104 36-37 0.26749730315341935 1104 38-39 0.4197644582866573 1104 40-41 0.532979354547436 1104 42-43 0.011858890022907076 1104 44-45 0.07114017882032186 1104 46-47 -0.08007120965790904 1104 48-49 -0.3712986916178096 1104 50-51 -0.26835076812407266 1104 52-53 -0.30966106063479515 1104 54-55 -0.47518521504768074 1104 56-57 -0.08616817680870525 1104 58-59 -0.11188033004447817 1104 60-61 0.08241570992954195 1104 62-63 0.13844410654383665 1104 64-65 0.2752815777116524 1104 66-67 0.6054526028921643 1104 68-69 0.3722132674373455 1104 70-71 0.5680952593513453 1104 72-73 0.35747000202235313 1104 74-75 0.6190117977666851 1104 76-77 0.484597095343009 1104 78-79 0.7718264844199716 1104 80-81 0.645951031696697 1104 82-83 0.7305477358275425 1104 84-85 0.5709018533915007 1104 86-87 0.6507505877909345 1104 88-89 0.8236966060416577 1104 90-91 1.1754636293350202 1104 92-93 1.2000415875486503 1104 94-95 0.9165887841184777 1104 96-97 0.8242611929929815 1104 98-99 0.7883317622819241 1104 100-101 0.13961192591144211 1105 1 0.1684899956777457 1105 2 0.1990170760154868 1105 3 0.03459745935377612 1105 4 -0.06611420800532386 1105 5 -0.011535853576255306 1105 6 0.11016087384686557 1105 7 0.016132285101221555 1105 8 0.10467995414266085 1105 9 -0.033287271422004494 1105 10-11 0.0040594416688506385 1105 12-13 0.0972828330908726 1105 14-15 -0.07386976680130175 1105 16-17 -0.06486264596978941 1105 18-19 -0.2017994155192966 1105 20-21 0.027977505751017873 1105 22-23 0.07412710328795669 1105 24-25 -0.06138738412131062 1105 26-27 0.19800037108392132 1105 28-29 -0.01891973971124372 1105 30-31 -0.1212479129744608 1105 32-33 -0.20720305932050564 1105 34-35 -0.001990227755960916 1105 36-37 -0.14138548072936175 1105 38-39 -0.20355666503446912 1105 40-41 -0.37407192750384155 1105 42-43 -0.5206502674862463 1105 44-45 -0.4635057308255881 1105 46-47 -0.32182945141614994 1105 48-49 -0.2650715853907002 1105 50-51 -0.1957011954745056 1105 52-53 -0.3351494611231942 1105 54-55 -0.4713695862320506 1105 56-57 -0.6168458324863622 1105 58-59 -0.7883150064791522 1105 60-61 -0.5445684170545846 1105 62-63 -0.281580313480589 1105 64-65 -0.688546261116187 1105 66-67 -0.17949855705899154 1105 68-69 -0.3787024834784063 1105 70-71 -0.4700610288049383 1105 72-73 -0.9319072873549352 1105 74-75 -0.833063904309018 1105 76-77 -0.4085652978193899 1105 78-79 -0.3348584972650137 1105 80-81 -0.9134201526744867 1105 82-83 -0.9326085523287446 1105 84-85 -0.6842874017977572 1105 86-87 -0.7384435514032042 1105 88-89 -0.41836688602183614 1105 90-91 -0.46067800680661364 1105 92-93 -0.8697081072010455 1105 94-95 -1.3001389326092365 1105 96-97 -0.5779060841742947 1105 98-99 -0.43190633295617076 1105 100-101 -0.32772629142677445 1106 1 -0.0934147662270135 1106 2 -0.07479244779403871 1106 3 0.07427999903631388 1106 4 -0.169288811179932 1106 5 0.05989271785231409 1106 6 -0.0961883325023436 1106 7 -0.11878834981941111 1106 8 -0.014367664904959554 1106 9 0.18496669683196387 1106 10-11 0.1508848384942567 1106 12-13 -0.09716161135357737 1106 14-15 0.14041594748442066 1106 16-17 -0.08271978882692821 1106 18-19 -0.05100576472563745 1106 20-21 -0.07916535139183623 1106 22-23 0.0046826588435138206 1106 24-25 0.04377134603741695 1106 26-27 0.13252418060773152 1106 28-29 0.05052470473320625 1106 30-31 0.007720340993792263 1106 32-33 -0.006806233923676075 1106 34-35 0.05554945478372275 1106 36-37 -0.024321988665867877 1106 38-39 -0.0011757126535201223 1106 40-41 0.006880453448538049 1106 42-43 -0.09803121986720242 1106 44-45 -0.2849343022541646 1106 46-47 -0.13928976887646627 1106 48-49 -0.3603096806287951 1106 50-51 -0.6103837351570363 1106 52-53 -0.6823716833454156 1106 54-55 -0.8265283163907782 1106 56-57 -0.45017916581969786 1106 58-59 -0.4133150064791522 1106 60-61 -0.42353667102283765 1106 62-63 -0.4184850753853553 1106 64-65 -0.31156213413206046 1106 66-67 -0.41759379515423234 1106 68-69 -0.4104485152244308 1106 70-71 -0.48990229864620716 1106 72-73 -0.3366691921168403 1106 74-75 -0.33306390430901445 1106 76-77 -0.23396212321621235 1106 78-79 -0.16422357663008924 1106 80-81 -0.177309041563376 1106 82-83 -0.45244982217001706 1106 84-85 -0.5870651795755322 1106 86-87 -0.37336418632383683 1106 88-89 -0.19217640983136164 1106 90-91 -0.12932880045741157 1106 92-93 -0.6038350913280297 1106 94-95 -0.23466274213305027 1106 96-97 -0.22076322703143703 1106 98-99 -0.10254125359109167 1106 100-101 -0.8932024819029678 1107 1 -0.13234655515880434 1107 2 -0.15469377769537118 1107 3 0.12340054815686585 1107 4 -0.11845226034338197 1107 5 0.008627166586762769 1107 6 -0.10648434279835328 1107 7 -0.13165836268942854 1107 8 -0.1813560818933766 1107 9 -0.1642396523743841 1107 10-11 -0.04549010788069552 1107 12-13 -0.17888619307815645 1107 14-15 -0.04791524084676979 1107 16-17 0.011499430392284182 1107 18-19 -0.196812285532161 1107 20-21 -0.333079980306465 1107 22-23 -0.3255404213795643 1107 24-25 -0.28076747850141004 1107 26-27 -0.22306354997999733 1107 28-29 -0.21379318458468077 1107 30-31 0.1094470677205166 1107 32-33 0.108594881477444 1107 34-35 0.08311273234699712 1107 36-37 0.3634943991505182 1107 38-39 0.322826861349057 1107 40-41 0.2908251123931933 1107 42-43 0.4573098354738505 1107 44-45 0.3211896788698212 1107 46-47 0.8510577214710224 1107 48-49 1.1038687835496717 1107 50-51 0.8304678306945235 1107 52-53 0.5869333359595998 1107 54-55 0.8050675652050998 1107 56-57 1.1673133266727973 1107 58-59 1.4316549634908142 1107 60-61 1.4471733246871494 1107 62-63 1.276012994112719 1107 64-65 1.3166553690854386 1107 66-67 1.1823096797492454 1107 68-69 1.3975737927978713 1107 70-71 1.080346521602614 1107 72-73 0.7814131759655218 1107 74-75 1.006060934815821 1107 76-77 0.6899975507434597 1107 78-79 0.7598969724904592 1107 80-81 1.0029783887240526 1107 82-83 1.114742370022178 1107 84-85 1.3397366222262654 1107 86-87 0.9043063413466861 1107 88-89 1.1781582105032626 1107 90-91 1.4115871154585058 1107 92-93 1.2399551274621885 1107 94-95 1.006060123589819 1107 96-97 1.145978514710304 1107 98-99 0.9330121569623202 1107 100-101 0.5296310659305803 1108 1 -0.19723536423366284 1108 2 -0.15295782247104484 1108 3 -0.03627740221875797 1108 4 -0.060207969540947204 1108 5 -0.09939115073387939 1108 6 -0.18755045874819132 1108 7 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539.0 12 886.0 13 1256.0 14 1542.0 15 2207.0 16 3003.0 17 4686.0 18 4370.0 19 2229.0 20 1667.0 21 1635.0 22 1799.0 23 1955.0 24 2225.0 25 2396.0 26 2705.0 27 3092.0 28 3673.0 29 4436.0 30 5320.0 31 6322.0 32 7820.0 33 9940.0 34 13716.0 35 21983.0 36 30900.0 37 58869.0 38 61041.0 39 5667.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.40661233610217 23.612123114404326 15.950541997825281 18.03072255166822 2 13.883747863422919 25.20174128704797 39.340202653638045 21.574308195891067 3 19.602885263483245 30.04986277496211 33.33805025005865 17.009201711495994 4 10.498739466069852 20.288451870691933 42.22807285402011 26.984735809218098 5 9.40764214987134 42.49395800206303 35.86805542625411 12.23034442181152 6 22.784197335935087 42.61386699039611 18.559453632087944 16.042482041580865 7 22.17869418366929 39.36738698950238 22.70115477569199 15.752764051136342 8 22.945069022145926 33.50227342973221 21.319222304561382 22.23343524356048 9 24.044731266082515 20.487679537642858 25.257599511426733 30.209989684847898 10-11 21.843731031477972 28.75060047591207 28.59252170092017 20.813146791689785 12-13 25.94670380617941 28.527167578397016 28.03282229264496 17.49330632277861 14-15 22.58284348135335 29.18760601563678 30.387456313644297 17.84209418936557 16-17 16.428707086279896 32.53492122011075 31.480674628444177 19.555697065165177 18-19 16.07506600580181 32.505948900896335 34.66431069089176 16.754674402410096 20-21 17.969032200404413 29.846538838223413 33.24588417983369 18.938544781538486 22-23 17.42888317066177 29.81190673910858 33.106238618886785 19.65297147134287 24-25 18.496740666538813 30.69936489080628 33.71590081795212 17.087993624702786 26-27 18.65111801693271 28.586936080600744 36.28206536614612 16.479880536320422 28-29 16.194044023728573 30.612168900374996 36.0600215240358 17.133765551860638 30-31 16.953940055226504 30.487598243030124 34.949847410256936 17.60861429148644 32-33 16.96414274383046 30.70545213508753 34.61459687119466 17.715808249887353 34-35 18.326607909991676 29.262015913835132 34.31921149609789 18.092164680075303 36-37 18.753887930745478 28.706618142807656 35.29179502270365 17.247698903743217 38-39 18.06122277947769 30.176159357266968 34.60109468728408 17.16152317597126 40-41 16.728863364655144 30.468560872690436 35.787495856657095 17.015079905997325 42-43 16.14921521052171 31.285624638050884 33.84808471890403 18.717075432523377 44-45 17.450499197485577 30.25516607021163 33.084820746952865 19.209513985349922 46-47 18.720953615157747 31.098437453451304 31.6218309649358 18.55877796645515 48-49 18.255873059614093 30.48870633491661 32.491607254906704 18.76381335056259 50-51 17.105836439671258 31.149338824817434 33.619203312765094 18.125621422746214 52-53 17.228352145142008 32.648945955305976 32.17228910727386 17.950412792278158 54-55 16.452853796683513 30.700797283055962 32.69884596908433 20.14750295117619 56-57 18.444199495786428 31.70550054554866 30.77713685637361 19.073163102291304 58-59 18.782061427600524 30.274739086313318 31.50236009347274 19.44083939261342 60-61 17.909369384467848 30.9227861419141 32.51138236362113 18.656462109996927 62-63 18.50320173241747 30.587111514133614 32.02402742373658 18.885659329712336 64-65 17.800166831760595 31.67513238547074 32.016057556957406 18.508643225811255 66-67 17.47965284618092 30.68970846452771 31.053918328358655 20.77672036093272 68-69 16.66837349341506 31.054316192448127 31.800602913273963 20.47670740086285 70-71 18.291892737313667 31.18974294045141 30.811024179163393 19.707340143071534 72-73 18.243296082104145 29.107162141529848 32.157207386691596 20.492334389674422 74-75 17.183293177476475 32.06653086911673 30.718485720775906 20.031690232630886 76-77 18.012225340339143 29.73377418199188 31.563283675662767 20.69071680200621 78-79 17.462276938659993 29.66511007835076 32.22584681383502 20.64676616915423 80-81 17.799960153650503 30.338526392620608 31.257785399478273 20.60372805425062 82-83 17.101803854976612 30.20637828819972 31.82031459469121 20.87150326213245 84-85 16.980900211144085 29.437656635770864 31.19719070370191 22.384252449383137 86-87 17.165357266008705 29.776050549389097 31.603433332401377 21.455158852200817 88-89 17.96071951294738 28.707803945245836 31.271110878492937 22.06036566331385 90-91 17.226066185953147 27.92773597389344 32.46218025635562 22.384017583797785 92-93 17.648592186551575 29.54937308452839 31.794501316392154 21.00753341252788 94-95 17.284582757683296 28.730305321054455 31.491563546177993 22.493548375084252 96-97 16.551350465671398 28.079929395204385 31.500873250241124 23.867846888883097 98-99 17.108352526947087 28.318974586227892 30.41180774981334 24.16086513701168 100-101 17.28033246877054 27.436121346632582 31.8133661532045 23.470180031392378 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1560.0 1 941.0 2 386.0 3 436.0 4 349.5 5 350.0 6 627.5 7 1172.0 8 1697.0 9 1751.5 10 1327.5 11 963.5 12 838.0 13 780.5 14 753.0 15 699.5 16 671.5 17 708.5 18 759.0 19 830.0 20 948.5 21 1026.5 22 1156.0 23 1327.0 24 1456.5 25 1872.0 26 2789.0 27 3748.5 28 3959.5 29 4067.0 30 4784.0 31 5642.5 32 6909.0 33 8550.5 34 10505.5 35 11680.0 36 12685.0 37 14314.5 38 13627.5 39 11612.0 40 10436.5 41 9475.5 42 8577.5 43 8141.0 44 7958.5 45 7557.0 46 7619.5 47 9961.0 48 19888.5 49 19100.5 50 8177.0 51 4786.5 52 2690.0 53 1266.0 54 646.5 55 389.5 56 286.0 57 270.5 58 261.0 59 255.5 60 244.5 61 212.5 62 167.5 63 111.0 64 65.5 65 62.5 66 62.0 67 61.0 68 56.0 69 43.5 70 36.0 71 26.5 72 24.0 73 27.0 74 23.0 75 20.0 76 19.0 77 13.0 78 13.0 79 13.0 80 5.5 81 1.0 82 1.0 83 0.5 84 0.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3411075568729821 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013033585688378138 16-17 0.0014895526501003586 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.8619408126254483E-4 26-27 0.0013033585688378138 28-29 0.0 30-31 0.0024205230564130825 32-33 0.0 34-35 0.010613062631965054 36-37 0.029046276676956993 38-39 0.021784707507717747 40-41 0.012661197525853048 42-43 0.009495898144389787 44-45 0.002234328975150538 46-47 3.7238816252508966E-4 48-49 5.585822437876345E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0035376875439883516 60-61 0.010985450794490145 62-63 0.003910075706513441 64-65 0.001117164487575269 66-67 0.003910075706513441 68-69 0.002792911218938172 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.21188886447677602 78-79 3.7238816252508966E-4 80-81 0.0020481348938879928 82-83 3.7238816252508966E-4 84-85 0.0 86-87 0.10594443223838801 88-89 0.3911937647326067 90-91 0.3790911494505413 92-93 0.0 94-95 0.0 96-97 0.0 98-99 5.585822437876345E-4 100-101 1.8619408126254483E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 268537.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.042027728022582 #Duplication Level Percentage of deduplicated Percentage of total 1 70.63404909530165 15.569176687011474 2 10.444155361456978 4.604207241460209 3 4.529404808163403 2.995117991189296 4 2.6220202395634473 2.3117857129557566 5 1.7283032893514219 1.9047654513158336 6 1.2383639404639217 1.6377631387853444 7 0.9444003311314221 1.4571548799606757 8 0.77883462012806 1.3733675433925305 9 0.5524488520214221 1.0959383623113388 >10 5.392711729823791 24.13745591855126 >50 0.6892939805037928 10.579547697337798 >100 0.38857258704870673 15.997795462077852 >500 0.03209947458228447 5.0979939449684775 >1k 0.02534169045969827 11.237929968682156 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3787 1.4102339714825145 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3595 1.3387354442776973 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2983 1.1108338888123424 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2717 1.0117786375806685 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2348 0.8743674056089106 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2195 0.8173920167425719 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2014 0.7499897593255306 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1945 0.7242949761112994 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1693 0.6304531591549768 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1582 0.5891180731146919 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1120 0.4170747420281004 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1102 0.4103717551026488 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1054 0.3924971233014445 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1022 0.3805807021006416 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1021 0.38020831393811655 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 956 0.35600308337398573 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 943 0.3511620372611595 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 926 0.34483143849823306 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 880 0.3277015830220789 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 878 0.3269568066970287 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 836 0.311316503870975 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 820 0.3053582932705735 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 760 0.28301500351906816 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 757 0.28189783903149285 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 679 0.2528515623545359 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 678 0.2524791741920108 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 600 0.2234328975150538 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 591 0.22008140405232798 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 590 0.2197090158898029 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 585 0.21784707507717746 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 582 0.21672991058960217 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 564 0.21002692366415054 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 534 0.19885527878839787 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 531 0.1977381143008226 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 480 0.17874631801204302 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 474 0.1765119890368925 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 466 0.17353288373669176 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 463 0.17241571924911653 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 462 0.17204333108659142 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 450 0.16757467313629035 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 427 0.15900974539821328 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 424 0.15789258091063801 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 419 0.15603064009801257 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 413 0.15379631112286204 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 400 0.14895526501003586 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 398 0.14821048868498568 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 387 0.14411421889720968 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 383 0.14262466624710934 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 381 0.14187988992205916 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 373 0.13890078462185845 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 362 0.13480451483408246 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 362 0.13480451483408246 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 353 0.13145302137135664 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 351 0.13070824504630646 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 351 0.13070824504630646 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 345 0.12847391607115594 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 332 0.12363286995832976 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 327 0.12177092914570431 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 317 0.11804704752045342 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 316 0.11767465935792834 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 313 0.11655749487035306 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 307 0.11432316589520253 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 306 0.11395077773267742 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 298 0.11097167243247673 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 296 0.11022689610742653 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 293 0.10910973161985127 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 291 0.10836495529480109 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 290 0.107992567132276 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 286 0.10650301448217564 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 285 0.10613062631965055 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 283 0.10538584999460036 No Hit CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC 272 0.10128958020682438 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 270 0.10054480388177421 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.585822437876345E-4 0.0 22-23 0.0 0.0 0.0 0.0027929112189381726 0.0 24-25 0.0 0.0 0.0 0.005399628356613799 0.0 26-27 0.0 0.0 0.0 0.010613062631965056 0.0 28-29 0.0 0.0 0.0 0.028301500351906813 0.0 30-31 0.0 0.0 0.0 0.0722433035298674 0.0 32-33 0.0 0.0 0.0 0.13610787340292027 0.0 34-35 0.0 0.0 0.0 0.2385146180973199 0.0 36-37 0.0 0.0 0.0 0.3863527186197805 0.0 38-39 0.0 0.0 0.0 0.5950762837150932 0.0 40-41 0.0 0.0 0.0 0.8227916450991857 0.0 42-43 0.0 0.0 0.0 1.0367286444698496 0.0 44-45 0.0 0.0 0.0 1.286042519280397 0.0 46-47 0.0 0.0 0.0 1.5984761876389473 0.0 48-49 0.0 0.0 0.0 1.9084893329410844 0.0 50-51 0.0 0.0 0.0 2.241962932482302 0.0 52-53 0.0 0.0 0.0 2.5339152519019725 0.0 54-55 0.0 0.0 0.0 2.839645933335071 0.0 56-57 0.0 0.0 0.0 3.2420113429434307 0.0 58-59 0.0 0.0 0.0 3.6767745226914723 0.0 60-61 0.0 0.0 0.0 4.2055657134771 0.0 62-63 0.0 0.0 0.0 4.688925548434666 0.0 64-65 0.0 0.0 0.0 5.136722313871086 0.0 66-67 0.0 0.0 0.0 5.6617896230314635 0.0 68-69 0.0 0.0 0.0 6.237501722295252 0.0 70-71 0.0 0.0 0.0 6.913013849115765 0.0 72-73 0.0 0.0 0.0 7.607890160387582 0.0 74-75 0.0 0.0 0.0 8.327902672629843 0.0 76-77 0.0 0.0 0.0 9.01924129635767 0.0 78-79 0.0 0.0 0.0 9.888953849935017 0.0 80-81 0.0 0.0 0.0 10.846550009868286 0.0 82-83 0.0 0.0 0.0 11.72408271485866 0.0 84-85 0.0 0.0 0.0 12.685588950498442 0.0 86-87 0.0 0.0 0.0 13.69140937747871 0.0 88-89 0.0 0.0 0.0 14.670604050838431 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTCAGT 35 2.7964372E-4 54.237083 3 TGTAGAT 45 9.654368E-4 42.1844 5 GGACACC 45 9.654368E-4 42.1844 6 GCAGATC 55 0.0025874667 34.514507 3 AGATCAG 60 0.0039629554 31.638298 5 GACACCT 60 0.0039629554 31.638298 7 GACATAT 110 2.6674861E-6 30.307428 1 GTACATA 65 0.005861817 29.204584 3 TCCTCAG 65 0.005861817 29.204584 2 AGGCTTA 100 4.969441E-5 28.474468 6 TAGGACA 170 3.6488927E-9 27.916147 4 CTTACAC 170 3.6488927E-9 27.916147 3 ATATCTC 35 0.008212256 27.260683 84-85 ATTGAGA 70 0.008272711 27.214832 1 AAGACGC 35 0.008424406 27.118542 52-53 AAAGTCA 70 0.008417035 27.118542 9 TGTACGT 35 0.008424406 27.118542 10-11 GTACGTA 35 0.008424406 27.118542 12-13 CATATAG 125 7.414774E-6 26.576172 3 ACACCAC 205 9.586074E-10 25.464972 6 >>END_MODULE