##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939152_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 265279 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.158271103253554 33.0 31.0 34.0 31.0 34.0 2 32.24283490212191 34.0 31.0 34.0 31.0 34.0 3 32.2685587626612 34.0 31.0 34.0 30.0 34.0 4 35.70698396782256 37.0 35.0 37.0 35.0 37.0 5 35.771983458924375 37.0 35.0 37.0 35.0 37.0 6 35.88674942230632 37.0 37.0 37.0 35.0 37.0 7 35.86010200581275 37.0 37.0 37.0 35.0 37.0 8 35.87165587928181 37.0 37.0 37.0 35.0 37.0 9 37.52136429947338 39.0 38.0 39.0 35.0 39.0 10-11 37.46020793202628 39.0 38.0 39.0 35.0 39.0 12-13 37.41175894058708 39.0 38.0 39.0 35.0 39.0 14-15 38.61640574640285 40.0 38.0 41.0 35.0 41.0 16-17 38.56636974656871 40.0 38.0 41.0 34.5 41.0 18-19 38.615568891619766 40.0 38.5 41.0 35.0 41.0 20-21 38.6433622714199 40.0 38.5 41.0 35.0 41.0 22-23 38.663199876356586 40.5 38.5 41.0 35.0 41.0 24-25 38.5827524983131 40.5 38.0 41.0 35.0 41.0 26-27 38.41046596225107 40.0 38.0 41.0 34.5 41.0 28-29 38.278878086844415 40.0 38.0 41.0 34.0 41.0 30-31 38.03451837499387 40.0 37.5 41.0 34.0 41.0 32-33 37.74751865017585 40.0 37.0 41.0 34.0 41.0 34-35 37.41944895751266 40.0 36.0 41.0 33.0 41.0 36-37 37.08705928475303 40.0 35.5 41.0 33.0 41.0 38-39 36.85450601065294 40.0 35.0 41.0 32.5 41.0 40-41 36.691138386378114 40.0 35.0 41.0 31.5 41.0 42-43 36.439505200185465 40.0 35.0 41.0 31.0 41.0 44-45 36.051645625925914 40.0 35.0 41.0 30.0 41.0 46-47 35.588991213024784 40.0 35.0 41.0 27.0 41.0 48-49 35.28637019892264 40.0 35.0 41.0 24.0 41.0 50-51 34.13729130462645 38.5 34.0 40.0 21.5 40.5 52-53 34.29054316398961 38.5 35.0 40.0 22.0 40.5 54-55 34.848947711654525 39.5 35.0 41.0 20.5 41.0 56-57 34.80042332789252 40.0 35.0 41.0 18.5 41.0 58-59 34.64155285567271 39.0 35.0 41.0 14.0 41.0 60-61 34.31485530328447 39.0 35.0 41.0 8.5 41.0 62-63 33.96337817920001 39.0 35.0 40.5 4.0 41.0 64-65 33.58198915104475 38.0 34.5 40.0 2.0 41.0 66-67 33.15255259556919 37.5 34.0 40.0 2.0 41.0 68-69 32.69188288556576 37.0 34.0 39.0 2.0 41.0 70-71 32.173076270643364 36.0 33.0 39.0 2.0 41.0 72-73 31.665742482443015 36.0 33.0 38.5 2.0 40.0 74-75 31.1829357016575 35.0 32.0 37.0 2.0 39.5 76-77 30.68976247648702 35.0 31.5 37.0 2.0 39.0 78-79 30.21577659746908 35.0 31.0 36.0 2.0 39.0 80-81 29.72589990161302 35.0 31.0 36.0 2.0 37.5 82-83 29.312324006046463 35.0 30.5 35.5 2.0 37.0 84-85 28.90495666826247 35.0 30.0 35.0 2.0 37.0 86-87 28.52571443649893 34.0 29.5 35.0 2.0 36.0 88-89 28.03282958696316 34.0 29.0 35.0 2.0 36.0 90-91 27.57250291202847 34.0 27.5 35.0 2.0 36.0 92-93 27.158436966363716 34.0 26.5 35.0 2.0 35.0 94-95 26.65949434369098 34.0 25.0 35.0 2.0 35.0 96-97 26.066784404344105 34.0 24.0 35.0 2.0 35.0 98-99 25.480060615427533 34.0 18.5 35.0 2.0 35.0 100-101 22.911046483136623 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27647424912905905 1101 2 0.39920508222863305 1101 3 0.21905640163880236 1101 4 0.17894048487023184 1101 5 0.10314238453349134 1101 6 0.007320303416143759 1101 7 0.08596190484992405 1101 8 0.2587420843411792 1101 9 0.299095429549439 1101 10-11 0.1285652386176821 1101 12-13 0.13560695959522917 1101 14-15 -0.01962780244473805 1101 16-17 -0.026446758582885366 1101 18-19 0.06503409515738667 1101 20-21 0.07458928179399038 1101 22-23 0.22404440587054353 1101 24-25 0.1826644298863016 1101 26-27 -0.13062191696898395 1101 28-29 0.08745791709813489 1101 30-31 -0.038138442261519856 1101 32-33 0.1891307745636155 1101 34-35 0.14345075968827103 1101 36-37 0.33687798726194984 1101 38-39 0.045577095524500066 1101 40-41 0.322358548729369 1101 42-43 -0.1500290101995887 1101 44-45 -0.15958418008300868 1101 46-47 -0.019679915008275373 1101 48-49 0.10386747369057758 1101 50-51 0.2461468868263026 1101 52-53 0.14719409362485436 1101 54-55 0.21253848893394434 1101 56-57 0.030216095439683954 1101 58-59 0.216285746510259 1101 60-61 0.051762133376762165 1101 62-63 0.043828102474755326 1101 64-65 -0.1620722242759456 1101 66-67 0.04665135935204745 1101 68-69 -0.06550683807024171 1101 70-71 -0.32094635309978514 1101 72-73 -0.37559884385948195 1101 74-75 -0.2690620564770292 1101 76-77 -0.6332207454567893 1101 78-79 -0.5611251436588098 1101 80-81 -0.7133299907937918 1101 82-83 -1.0056659846809843 1101 84-85 -0.8595597268886586 1101 86-87 -1.049124993334818 1101 88-89 -1.347721978990844 1101 90-91 -1.5315067458983123 1101 92-93 -1.6843877672607803 1101 94-95 -1.247679371108351 1101 96-97 -1.6690288842270498 1101 98-99 -1.2976427334535678 1101 100-101 -1.7809662591355675 1102 1 0.6579821856369961 1102 2 0.42454238381593257 1102 3 0.502568306400704 1102 4 0.4374722309019745 1102 5 0.32131698770809436 1102 6 0.11849093833677671 1102 7 0.4294936508816676 1102 8 0.4022936716427665 1102 9 0.5083017787557864 1102 10-11 0.45939857195101297 1102 12-13 0.3813708484841172 1102 14-15 0.41622537215843636 1102 16-17 0.30863260649648083 1102 18-19 0.4208575078557999 1102 20-21 0.3882301548098681 1102 22-23 0.5003539296800739 1102 24-25 0.3915136362355085 1102 26-27 0.25910030525323435 1102 28-29 0.31681307582828566 1102 30-31 0.32360758948450297 1102 32-33 0.24709704440488167 1102 34-35 0.6483217914342987 1102 36-37 0.5108760031349604 1102 38-39 0.15020011139751688 1102 40-41 0.4789656915865095 1102 42-43 0.2197626564670827 1102 44-45 -0.3182944975433273 1102 46-47 0.014724846896491783 1102 48-49 0.2758119181350196 1102 50-51 0.2014445058739227 1102 52-53 0.16297782378358505 1102 54-55 0.05932420321965992 1102 56-57 -0.0012422378936491896 1102 58-59 0.08239685762136872 1102 60-61 0.1521887206783461 1102 62-63 0.26536580088745865 1102 64-65 0.07022936302564631 1102 66-67 0.45655215300283913 1102 68-69 0.06963205081864743 1102 70-71 0.06297229769386803 1102 72-73 0.5236571085214692 1102 74-75 0.5860966736816984 1102 76-77 0.6176026672416235 1102 78-79 0.43336890396023975 1102 80-81 0.48372358063477705 1102 82-83 0.2595423486523494 1102 84-85 0.5830791620002316 1102 86-87 0.5473035780937536 1102 88-89 0.37904389402503114 1102 90-91 0.5942670636254981 1102 92-93 0.2781816771836638 1102 94-95 0.14297539079641197 1102 96-97 -0.43465388422704976 1102 98-99 -0.7562935271043578 1102 100-101 -1.3290317353260441 1103 1 -0.12167050112578437 1103 2 0.03098897621435981 1103 3 -0.04449099897281883 1103 4 -0.26428071798400055 1103 5 -0.03861092841248137 1103 6 -0.09673678119139595 1103 7 -0.05780975671990518 1103 8 -0.061951085893277025 1103 9 0.15705669356574958 1103 10-11 -0.19369775832421965 1103 12-13 -0.092109656103041 1103 14-15 0.18182989378360048 1103 16-17 0.38137179391457465 1103 18-19 0.1564612103459666 1103 20-21 0.25022638678889564 1103 22-23 0.3697018982252871 1103 24-25 0.35227707004939646 1103 26-27 0.42939847039084356 1103 28-29 0.2233702514508309 1103 30-31 0.33900732736130834 1103 32-33 0.2689065136054083 1103 34-35 0.274816712797346 1103 36-37 -0.11574178847709504 1103 38-39 0.12378300785885443 1103 40-41 0.4671291909312032 1103 42-43 0.24599545463222228 1103 44-45 -0.11764738089850368 1103 46-47 -0.10708052662906908 1103 48-49 -0.24425688920442923 1103 50-51 0.17461783483853566 1103 52-53 0.08411560228947934 1103 54-55 0.018777021044037667 1103 56-57 0.017249734583415943 1103 58-59 -0.2237958029290894 1103 60-61 -0.17085764236227874 1103 62-63 0.07305338280094986 1103 64-65 0.25191350457216544 1103 66-67 0.22887358157426974 1103 68-69 0.2413637021947963 1103 70-71 0.4565052269206049 1103 72-73 0.7277650705136054 1103 74-75 0.6501530301692462 1103 76-77 0.6571054523005984 1103 78-79 0.8029421346286547 1103 80-81 0.37602780737134367 1103 82-83 0.3239058807624424 1103 84-85 -0.05424881965114281 1103 86-87 -0.13077636948160531 1103 88-89 0.03562154802240869 1103 90-91 0.48782047122707084 1103 92-93 -0.07975983657780361 1103 94-95 -0.4011350286007094 1103 96-97 -0.5933408108325544 1103 98-99 -0.45894752448312914 1103 100-101 -0.16338216127623895 1104 1 0.017811515599071015 1104 2 0.22364952667307847 1104 3 0.3185844252376242 1104 4 0.3165019401307987 1104 5 0.24973038846662376 1104 6 0.19359365641516035 1104 7 0.26759415657067365 1104 8 0.0589387411749982 1104 9 0.2067847117519932 1104 10-11 0.3072968020395095 1104 12-13 0.07343390158145979 1104 14-15 0.2696150687450327 1104 16-17 0.4268374105419994 1104 18-19 0.04421796929700861 1104 20-21 0.1265558501322417 1104 22-23 0.43248098404163215 1104 24-25 0.29203512801806397 1104 26-27 0.17558260613818533 1104 28-29 0.4110174057903677 1104 30-31 0.6719008891052383 1104 32-33 0.6095535867563342 1104 34-35 0.6032246043293696 1104 36-37 0.2883135821488665 1104 38-39 -0.11342978746468191 1104 40-41 0.23793376996830773 1104 42-43 0.5276996033697401 1104 44-45 0.3848897628865089 1104 46-47 0.6497596130153269 1104 48-49 0.5883119181350196 1104 50-51 0.6643474767968058 1104 52-53 0.9479217239099995 1104 54-55 0.9750163650401404 1104 56-57 0.7782101957346725 1104 58-59 1.0613791585063197 1104 60-61 1.0385074627769626 1104 62-63 1.0786756934285435 1104 64-65 0.9904253175136333 1104 66-67 1.1226788913087873 1104 68-69 0.8956829357743956 1104 70-71 0.6503024177950039 1104 72-73 0.7308908316567369 1104 74-75 0.32803725522405003 1104 76-77 0.8172905623111575 1104 78-79 0.9642435879046118 1104 80-81 0.6488823985867391 1104 82-83 0.9328000920151815 1104 84-85 0.7238424363365148 1104 86-87 0.36636173232889746 1104 88-89 0.5248640583739537 1104 90-91 0.627018327847999 1104 92-93 0.4810301285110974 1104 94-95 0.10184865249046737 1104 96-97 -0.04292268953678402 1104 98-99 0.08836513281978498 1104 100-101 -0.09394561016801717 1105 1 -0.33714768449287646 1105 2 -0.2547054516819003 1105 3 -0.13609511351271664 1105 4 -0.18671608078633284 1105 5 -0.0363669949758858 1105 6 -0.09836512226927852 1105 7 -0.40627474738672475 1105 8 -0.44659702099792753 1105 9 -0.389966619512613 1105 10-11 -0.16857761852517683 1105 12-13 -0.36572060173233467 1105 14-15 -0.46459172740865995 1105 16-17 -0.584224536360658 1105 18-19 -0.7108389207156307 1105 20-21 -0.5056415984368883 1105 22-23 -0.5502413084151669 1105 24-25 -0.5952287880069136 1105 26-27 -0.5258022921493648 1105 28-29 -0.5803054306652129 1105 30-31 -0.6709811551042364 1105 32-33 -0.8645777391449059 1105 34-35 -1.0212534250159138 1105 36-37 -0.6040455336615693 1105 38-39 -0.37915594920854545 1105 40-41 -0.6126198062489863 1105 42-43 -0.4747043348749145 1105 44-45 -0.4418064023052253 1105 46-47 -0.5748314301597901 1105 48-49 -0.3640690342459294 1105 50-51 -0.653939693260277 1105 52-53 -0.4489675225367691 1105 54-55 -0.09083813444267719 1105 56-57 -0.1698704846468928 1105 58-59 -0.4527763025517899 1105 60-61 -0.17106614945151932 1105 62-63 0.0016347402813963186 1105 64-65 -0.24308232528604634 1105 66-67 -0.4074900547893705 1105 68-69 -0.4405140530774574 1105 70-71 -0.5936159057693366 1105 72-73 -0.2894516577122985 1105 74-75 -0.2163925038074801 1105 76-77 -0.23541410765015058 1105 78-79 0.02069303815937218 1105 80-81 0.10770897024517012 1105 82-83 -0.02921576823076677 1105 84-85 -0.4457357730647047 1105 86-87 -0.46730681151663944 1105 88-89 -0.2082125994814632 1105 90-91 -0.06198293637450192 1105 92-93 0.01390949103647543 1105 94-95 -0.02057980400878634 1105 96-97 -0.06946178465995345 1105 98-99 -0.7525056483164825 1105 100-101 -0.7913558695251766 1106 1 -0.11982811881265576 1106 2 0.17037083814848586 1106 3 0.29845530874262494 1106 4 0.11209166884108868 1106 5 0.15826078161909862 1106 6 0.3837866057840884 1106 7 0.25370325275520855 1106 8 0.2835143976380792 1106 9 0.05572566634361209 1106 10-11 0.025996932606751955 1106 12-13 0.19924994684477326 1106 14-15 0.35335944709989064 1106 16-17 0.3706934964262274 1106 18-19 0.30116342120474116 1106 20-21 0.3374763491892523 1106 22-23 0.1257930397503273 1106 24-25 0.29183565028702674 1106 26-27 0.29291178066306855 1106 28-29 0.30821383695241167 1106 30-31 0.23402913515195678 1106 32-33 0.2579650186437519 1106 34-35 0.35766751742961844 1106 36-37 0.23427325137851795 1106 38-39 0.14690678587059125 1106 40-41 0.10506346676216083 1106 42-43 0.15803109909003865 1106 44-45 0.309559717913352 1106 46-47 -0.1197892046257536 1106 48-49 -0.003901196619082725 1106 50-51 -0.18109355033216445 1106 52-53 -0.111722117668414 1106 54-55 -0.030986101229991903 1106 56-57 0.2674565325981533 1106 58-59 0.40001980844103713 1106 60-61 0.30825575814908035 1106 62-63 0.34766234655490535 1106 64-65 0.35155254803735403 1106 66-67 0.32518505698410394 1106 68-69 0.31911975573667917 1106 70-71 0.3820955412535838 1106 72-73 0.6869694621514526 1106 74-75 0.7839889453444648 1106 76-77 0.7130725150167976 1106 78-79 0.8361426159040342 1106 80-81 0.6917519764193223 1106 82-83 0.8581591519310372 1106 84-85 0.7618701456067889 1106 86-87 0.19103894109140995 1106 88-89 -0.15762179684148236 1106 90-91 -0.09828270218246615 1106 92-93 -0.017156437570434946 1106 94-95 -0.12524767711927964 1106 96-97 0.1588399682319661 1106 98-99 0.18398750334060665 1106 100-101 0.24389712884257264 1107 1 0.03327137013670267 1107 2 0.06373049833299405 1107 3 0.21441402647010932 1107 4 0.17066338185628638 1107 5 0.21583694143857457 1107 6 0.056515085242509144 1107 7 0.13889937673476282 1107 8 0.2651298486236797 1107 9 0.3478043814162888 1107 10-11 0.16764755980526047 1107 12-13 0.3071679132614449 1107 14-15 0.35128537794212633 1107 16-17 0.2801479332749679 1107 18-19 0.22698641184653923 1107 20-21 0.39190822016556126 1107 22-23 0.44811997941980053 1107 24-25 0.23445030483006946 1107 26-27 0.21937358460545653 1107 28-29 0.6511736166033089 1107 30-31 0.35585166120225864 1107 32-33 0.11561750420823813 1107 34-35 0.4592222396702823 1107 36-37 0.40180826166589867 1107 38-39 0.4784425926005227 1107 40-41 0.5804152288913116 1107 42-43 0.9417388710419772 1107 44-45 0.9502313844693973 1107 46-47 1.3971351997015802 1107 48-49 1.0883119181350196 1107 50-51 1.2493587626697575 1107 52-53 1.2796547324012764 1107 54-55 0.7977206751687618 1107 56-57 1.1700001710132284 1107 58-59 1.2073968576213687 1107 60-61 1.0825223672948923 1107 62-63 0.8849707025647291 1107 64-65 1.2396191837312571 1107 66-67 0.9596428123435032 1107 68-69 0.8435439941384857 1107 70-71 0.9667009703370084 1107 72-73 1.0028087134954404 1107 74-75 1.0863135620564854 1107 76-77 1.253185012527915 1107 78-79 1.2612770155854562 1107 80-81 1.3835121144693652 1107 82-83 1.482404190757613 1107 84-85 1.3172643846722991 1107 86-87 1.5107940350052615 1107 88-89 1.2615807650487412 1107 90-91 1.5259291515375892 1107 92-93 1.3660485800176723 1107 94-95 1.5148847314557479 1107 96-97 1.5455460145583721 1107 98-99 1.632442736631905 1107 100-101 1.7063481808104513 1108 1 -0.13833477864871924 1108 2 -0.04588172332692153 1108 3 0.08348348497213465 1108 4 0.021735623759120415 1108 5 0.09140627342237906 1108 6 0.2027543311939226 1108 7 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0.029593694261414782 2316 84-85 -0.2712940674615254 2316 86-87 -0.20719864772575391 2316 88-89 -0.07670529658606284 2316 90-91 0.15068400008846794 2316 92-93 0.13206800862842627 2316 94-95 -0.5461307403245961 2316 96-97 -0.19169177374546464 2316 98-99 -0.5216213296134633 2316 100-101 -0.8287471845368906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1487.0 3 241.0 4 192.0 5 192.0 6 204.0 7 235.0 8 257.0 9 374.0 10 462.0 11 671.0 12 1127.0 13 1943.0 14 1801.0 15 2123.0 16 3009.0 17 4900.0 18 3751.0 19 1877.0 20 1755.0 21 1549.0 22 1637.0 23 1826.0 24 2012.0 25 2203.0 26 2489.0 27 2877.0 28 3427.0 29 3998.0 30 4768.0 31 5604.0 32 6720.0 33 8895.0 34 12315.0 35 20300.0 36 28920.0 37 52995.0 38 64735.0 39 11407.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14553510952689 23.169601132162573 15.497988822164958 18.18687493614558 2 13.912108232541465 24.517236824725927 39.90225861841236 21.668396324320245 3 19.705866813594884 29.996377112407636 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29.696720445524385 33.10720053124859 19.6626118715948 24-25 18.580061848006356 30.679856379797325 33.558109390595256 17.181972381601067 26-27 18.667642752562223 28.61786991894462 36.04587100572067 16.668616322772486 28-29 16.34376102529852 30.702355145265088 35.80262286545504 17.15126096398135 30-31 17.154736728915015 30.549104759461287 34.657722121713476 17.638436389910222 32-33 17.17252893403888 30.798834149240218 34.46409538092947 17.56454153579143 34-35 18.5607170885298 29.43126483297246 33.96412666294759 18.043891415550156 36-37 18.894907662663204 28.62694275975619 35.05143114819636 17.42671842938425 38-39 18.42986150182713 30.008884645476126 34.46333142000883 17.097922432687916 40-41 16.86665530367593 30.563036191125505 35.677518322822564 16.892790182376004 42-43 16.256949903861635 31.211343379713384 33.70809930193129 18.823607414493686 44-45 17.45100713863502 30.42161142181603 32.99345246214705 19.1339289774019 46-47 18.695841621672823 31.16245145401156 31.574500331533578 18.56720659278204 48-49 18.214353378045544 30.52536849683604 32.30552082149217 18.954757303626234 50-51 17.354426182920477 31.104059110500636 33.4430919338796 18.098422772699287 52-53 17.386848038194117 32.332379743478015 32.29789894473599 17.982873273591878 54-55 16.665561565202566 30.54645688683445 32.84803591895501 19.939945629007966 56-57 18.386692323426693 31.60819400146971 30.927128235819968 19.077985439283633 58-59 18.848797153240575 30.012366510806963 31.773221296154265 19.365615039798197 60-61 18.145442156268928 30.333320714718354 32.91471078134464 18.60652634766808 62-63 18.7649045446194 30.020854524067943 32.34856793252302 18.86567299878964 64-65 18.041334778357974 31.075734954186153 32.430824497714994 18.452105769740882 66-67 17.628411614458198 30.286533928557898 31.46889850612783 20.616155950856076 68-69 16.87604348872511 30.698893451865437 32.271446358283576 20.15361670112588 70-71 18.347425989865414 30.925453573824424 31.171806601503132 19.55531383480703 72-73 18.280692513059936 28.797235101627827 32.47258691940829 20.449485465903944 74-75 17.18688415244217 31.625761756056146 31.352514931934632 19.83483915956705 76-77 18.26425974724694 29.429951105107914 31.75572634773532 20.550062799909828 78-79 17.643270844302688 29.456273461796336 32.54650691495608 20.353948778944897 80-81 18.030635070659162 29.926869044259284 31.608445384756713 20.434050500324837 82-83 17.386840240811395 29.814428224489564 32.20947105603146 20.589260478667583 84-85 17.231470396581248 29.168118928912396 31.708883298731607 21.89152737577475 86-87 17.602309493320806 29.407965024208764 32.02527713162142 20.96444835084901 88-89 18.480567111474837 28.218797123523302 31.72099843142604 21.579637333575818 90-91 17.611771749922315 27.48857461176417 33.04949864714308 21.850154991170427 92-93 18.34896284351604 28.926878904614195 32.323999840831014 20.40015841103875 94-95 17.77764303741824 28.176609242009686 32.109996134635935 21.93575158593614 96-97 17.306573644409664 27.377458271673245 31.987517029553263 23.328451054363832 98-99 18.031988570367467 27.73448084418599 30.878577207812018 23.35495337763453 100-101 18.154556114823073 26.66948300064599 32.321600081837865 22.854360802693073 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1719.0 1 1051.0 2 423.0 3 450.0 4 354.5 5 339.5 6 598.5 7 1161.0 8 1718.0 9 1783.0 10 1355.5 11 981.5 12 919.0 13 903.0 14 868.0 15 833.0 16 799.0 17 790.5 18 789.5 19 823.0 20 912.5 21 1041.5 22 1178.0 23 1308.0 24 1413.5 25 1772.5 26 2692.0 27 3677.5 28 3875.5 29 3929.5 30 4736.0 31 5616.0 32 6803.0 33 8379.5 34 10344.5 35 11571.5 36 12512.0 37 14094.5 38 13487.5 39 11477.0 40 10171.5 41 9340.0 42 8408.0 43 7930.0 44 7801.5 45 7337.5 46 7409.5 47 9474.0 48 19319.0 49 19254.5 50 8285.0 51 3910.5 52 1869.0 53 1190.5 54 859.5 55 572.5 56 430.5 57 342.5 58 298.0 59 298.0 60 302.0 61 294.5 62 232.0 63 174.0 64 147.5 65 128.5 66 111.0 67 113.0 68 102.0 69 78.5 70 77.0 71 69.5 72 56.0 73 53.0 74 50.0 75 41.0 76 29.5 77 25.5 78 19.5 79 13.0 80 10.5 81 7.0 82 5.0 83 5.0 84 4.0 85 1.5 86 1.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3792233836828396 2 0.11082671451566088 3 0.11195759935765741 4 0.12703606391761127 5 0.12854391037360666 6 0.07162270665978084 7 0.08142370862375084 8 0.07765409248376237 9 0.07690016925576468 10-11 0.07463839957177161 12-13 0.08029282378175431 14-15 0.07294207230877679 16-17 0.07501536118577046 18-19 0.08406243992174277 20-21 0.08387395911474334 22-23 0.09160167220171969 24-25 0.10347596304268336 26-27 0.10366444384968278 28-29 0.09932938528869606 30-31 0.08990534493872489 32-33 0.0891514217107272 34-35 0.330406854669989 36-37 0.5211494313534053 38-39 0.5053170435654538 40-41 0.47647948009454194 42-43 0.5038091971094584 44-45 0.5137986798804278 46-47 0.5104060253544381 48-49 0.5160604495644209 50-51 0.5151180455294238 52-53 0.5141756414944266 54-55 0.509463621319441 56-57 0.48288782753252235 58-59 0.47478315283154715 60-61 0.42182004606470924 62-63 0.4928773103034918 64-65 0.5220918353884024 66-67 0.528123221212384 68-69 0.5430132049653383 70-71 0.5390551080183505 72-73 0.5275577787913857 74-75 0.5334006838083678 76-77 0.507201851635448 78-79 0.4847726356025166 80-81 0.49023857900549983 82-83 0.5043746395304566 84-85 0.49872021532047395 86-87 0.49947413854847156 88-89 0.5070133708284486 90-91 0.5251075283003932 92-93 0.5309504333173753 94-95 0.5266153747563885 96-97 0.5275577787913857 98-99 0.5290656252473811 100-101 0.5060709667934514 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 265279.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.596289189871793 #Duplication Level Percentage of deduplicated Percentage of total 1 72.75385008626749 17.16720886312147 2 9.64917886126909 4.5536962971060655 3 4.235094894242444 2.997975716132826 4 2.421879992331778 2.2858952272890054 5 1.6071314460987922 1.896116918414198 6 1.2221228193494793 1.7302538082547054 7 0.9121988625471277 1.5067155711533895 8 0.7013227682280018 1.323889188363949 9 0.5495558821649945 1.1670731569404287 >10 4.941210300977699 24.082946633544307 >50 0.613457728928366 10.260518171434603 >100 0.34187488018403733 15.307657221265158 >500 0.03035337721260144 5.406760429585455 >1k 0.020768100198095725 10.31329279739444 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3482 1.3125803399439835 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3471 1.3084337621899962 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2827 1.0656704827747392 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2724 1.0268434365328578 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2270 0.8557028637773816 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2196 0.8278077043414669 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1858 0.7003946788098567 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1819 0.6856931758639018 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1749 0.6593058628839825 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1627 0.6133165459761233 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1224 0.4614010155345881 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1072 0.40410285020676345 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1040 0.39204007855880035 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 983 0.3705532665608661 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 982 0.37017630494686726 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 977 0.368291496876873 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 912 0.343788991966948 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 882 0.33248014354698263 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 874 0.32946445063499186 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 865 0.3260717961090022 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 823 0.3102394083210507 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 795 0.29968448312908297 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 794 0.2993075215150841 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 692 0.26085743688720175 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 660 0.2487946652392387 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 657 0.24766378039724216 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 633 0.23861670166126983 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 627 0.23635493197727678 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 615 0.23183139260929062 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 532 0.20054357864738634 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 523 0.19715092412139673 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 517 0.19488915443740365 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 498 0.18772688377142555 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 493 0.18584207570143133 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 485 0.18282638278944055 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 482 0.18169549794744402 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 470 0.17717195857945786 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 448 0.16887880307148323 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 446 0.16812487984348554 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 438 0.16510918693149476 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 433 0.16322437886150054 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 432 0.16284741724750168 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 420 0.15832387787951555 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 409 0.15417730012552822 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 405 0.15266945366953283 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 397 0.14965376075754205 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 388 0.14626110623155245 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 369 0.13909883556557434 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 355 0.1338213729695905 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 345 0.13005175682960204 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 344 0.1296747952156032 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 341 0.12854391037360666 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 339 0.12778998714560896 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 332 0.12515125584761705 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 329 0.12402037100562051 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 326 0.12288948616362395 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 323 0.12175860132162741 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 318 0.11987379325163318 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 316 0.11911987002363549 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 316 0.11911987002363549 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 307 0.11572721549764588 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 304 0.11459633065564934 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 302 0.11384240742765164 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 292 0.11007279128766319 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 286 0.1078110216036701 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 276 0.10404140546368162 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 274 0.10328748223568393 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 270 0.10177963577968854 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 266 0.10027178932369317 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.654424209982698E-4 0.0 24-25 0.0 0.0 0.0 0.0035811353329890418 0.0 26-27 0.0 0.0 0.0 0.010931886805966549 0.0 28-29 0.0 0.0 0.0 0.02789515943591464 0.0 30-31 0.0 0.0 0.0 0.07313055311577622 0.0 32-33 0.0 0.0 0.0 0.1396642779865726 0.0 34-35 0.0 0.0 0.0 0.24954858846723638 0.0 36-37 0.0 0.0 0.0 0.39825994518978136 0.0 38-39 0.0 0.0 0.0 0.5952223885041785 0.0 40-41 0.0 0.0 0.0 0.8219647993244847 0.0 42-43 0.0 0.0 0.0 1.027408878953856 0.0 44-45 0.0 0.0 0.0 1.247365980722183 0.0 46-47 0.0 0.0 0.0 1.5566629850082365 0.0 48-49 0.0 0.0 0.0 1.8584207570143132 0.0 50-51 0.0 0.0 0.0 2.1925972278242907 0.0 52-53 0.0 0.0 0.0 2.4945434806373665 0.0 54-55 0.0 0.0 0.0 2.8185419878693754 0.0 56-57 0.0 0.0 0.0 3.206435488674188 0.0 58-59 0.0 0.0 0.0 3.6487999427018347 0.0 60-61 0.0 0.0 0.0 4.170703297283238 0.0 62-63 0.0 0.0 0.0 4.646805815763781 0.0 64-65 0.0 0.0 0.0 5.105945061614376 0.0 66-67 0.0 0.0 0.0 5.608623373881837 0.0 68-69 0.0 0.0 0.0 6.202526396737021 0.0 70-71 0.0 0.0 0.0 6.900093863441886 0.0 72-73 0.0 0.0 0.0 7.594457156427762 0.0 74-75 0.0 0.0 0.0 8.353469366214439 0.0 76-77 0.0 0.0 0.0 9.07214668330324 0.0 78-79 0.0 0.0 0.0 9.945189781324569 0.0 80-81 0.0 0.0 0.0 10.884389642602693 0.0 82-83 0.0 0.0 0.0 11.722186829715131 0.0 84-85 0.0 0.0 0.0 12.70869537355011 0.0 86-87 0.0 0.0 0.0 13.743831965590944 0.0 88-89 0.0 0.0 0.0 14.7391991073549 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATATG 30 1.3008014E-4 63.31933 1 TGCGGGT 25 0.0016055354 38.045605 50-51 AGCAGAT 75 7.075887E-6 37.86259 7 TAGGGTC 90 5.7876787E-7 36.852566 4 GTCTTCT 95 8.9154855E-7 34.87344 8 GGTAATG 55 0.002622231 34.42054 8 CCGACGC 35 2.0340244E-4 33.978954 94-95 AGGGTCT 100 1.3196168E-6 33.16731 5 CCGAGCA 30 0.00392045 31.71369 86-87 CGGGTAT 30 0.003927734 31.701668 52-53 GCGGGTA 30 0.003927734 31.701668 52-53 AAGACAT 60 0.0039938944 31.587915 5 ATAGGGT 105 1.9315448E-6 31.587914 3 TATAAGG 75 3.1172932E-4 31.587914 2 GCTTTGC 75 3.1380763E-4 31.55216 6 AAGCAGA 95 3.5679608E-5 29.89152 6 CCTTATA 80 4.5502768E-4 29.613667 2 TGGCCTA 80 4.5502768E-4 29.613667 5 ATAAGGC 80 4.5502768E-4 29.613667 3 TAGGCAT 80 4.5502768E-4 29.613667 5 >>END_MODULE