##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939152_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 265279 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37248707964068 34.0 31.0 34.0 31.0 34.0 2 32.58537992076267 34.0 31.0 34.0 31.0 34.0 3 32.66931419373565 34.0 31.0 34.0 31.0 34.0 4 36.14278175053434 37.0 35.0 37.0 35.0 37.0 5 36.12654978343555 37.0 35.0 37.0 35.0 37.0 6 36.19611051006676 37.0 37.0 37.0 35.0 37.0 7 36.16003905322321 37.0 36.0 37.0 35.0 37.0 8 36.16751797164495 37.0 37.0 37.0 35.0 37.0 9 37.84132931743561 39.0 38.0 39.0 35.0 39.0 10-11 37.771706392138086 39.0 38.0 39.0 35.0 39.0 12-13 37.721131337195935 39.0 37.5 39.0 35.0 39.0 14-15 38.94894243419193 40.0 38.0 41.0 35.5 41.0 16-17 39.06152390502075 40.0 38.0 41.0 36.0 41.0 18-19 39.20320681245029 40.5 38.5 41.0 36.0 41.0 20-21 39.21869993478565 41.0 39.0 41.0 36.0 41.0 22-23 39.14053882893105 41.0 39.0 41.0 35.0 41.0 24-25 39.02470606418149 40.5 38.5 41.0 35.0 41.0 26-27 38.78520161791924 40.0 38.0 41.0 35.0 41.0 28-29 38.62550559976478 40.0 38.0 41.0 35.0 41.0 30-31 38.375466961199336 40.0 37.5 41.0 34.0 41.0 32-33 38.139296740412924 40.0 37.0 41.0 34.0 41.0 34-35 37.97196347995883 40.0 37.0 41.0 34.0 41.0 36-37 37.708542704096445 40.0 36.5 41.0 33.0 41.0 38-39 37.5134801473166 40.0 36.0 41.0 33.0 41.0 40-41 37.32216270417183 40.0 36.0 41.0 33.0 41.0 42-43 37.067112360948286 40.0 36.0 41.0 33.0 41.0 44-45 36.685099461321855 40.0 35.5 41.0 31.5 41.0 46-47 36.15802796301253 40.0 35.0 41.0 30.0 41.0 48-49 35.61809453443356 40.0 35.0 41.0 27.5 41.0 50-51 35.34248093516637 39.0 35.0 41.0 26.0 41.0 52-53 35.087801899132614 39.0 35.0 41.0 24.0 41.0 54-55 34.89734958289198 39.0 35.0 40.0 24.0 41.0 56-57 34.72144421533555 39.0 35.0 40.0 23.0 41.0 58-59 34.43449538033542 38.5 35.0 40.0 22.0 41.0 60-61 34.038634795818744 38.0 34.0 40.0 20.0 41.0 62-63 33.63592670358378 38.0 34.0 40.0 16.5 41.0 64-65 33.154955725858436 37.0 33.5 39.5 10.5 41.0 66-67 32.67921886014347 37.0 33.0 39.0 7.0 40.5 68-69 32.15042464725817 36.0 32.5 39.0 2.0 40.0 70-71 31.61082482970759 35.5 31.5 38.5 2.0 40.0 72-73 30.987760056393455 35.0 31.0 37.0 2.0 39.0 74-75 30.441222260337227 35.0 31.0 37.0 2.0 39.0 76-77 28.38675696153861 32.5 28.0 35.0 2.0 37.0 78-79 29.2964105715115 34.0 29.0 36.0 2.0 37.0 80-81 29.28118320711402 34.0 29.5 35.5 2.0 37.0 82-83 28.883236140063858 34.0 29.0 35.0 2.0 37.0 84-85 28.448769031849487 34.0 29.0 35.0 2.0 36.0 86-87 27.83329061101708 34.0 28.0 35.0 2.0 36.0 88-89 27.132507661744803 33.0 26.0 35.0 2.0 35.0 90-91 26.569189419441418 33.0 25.0 35.0 2.0 35.0 92-93 26.17898325913472 33.0 25.0 34.5 2.0 35.0 94-95 25.690335458140297 32.0 23.5 34.0 2.0 35.0 96-97 24.93493077099959 32.0 19.0 34.0 2.0 35.0 98-99 23.8766317725866 31.5 2.0 34.0 2.0 35.0 100-101 18.403369659867536 24.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28500001862232693 1101 2 0.28468413087794175 1101 3 0.05428199119956645 1101 4 0.26104349566923446 1101 5 0.06241813541147678 1101 6 0.19723434428954079 1101 7 0.2763741702453828 1101 8 0.18801836124907112 1101 9 0.32442596051900097 1101 10-11 0.25388995127620717 1101 12-13 0.04169767352882303 1101 14-15 0.10650851457438648 1101 16-17 0.1320106982474627 1101 18-19 0.14446915130410076 1101 20-21 0.21233168424003424 1101 22-23 0.10013731946420279 1101 24-25 0.27052502331283534 1101 26-27 0.058881664898706276 1101 28-29 -0.2155936214901999 1101 30-31 -0.18288207836628345 1101 32-33 -0.011588198362368018 1101 34-35 -0.5094563113558479 1101 36-37 -0.21636303284612524 1101 38-39 -0.15843144746963844 1101 40-41 0.1275364261248555 1101 42-43 0.21937629489177368 1101 44-45 -0.009874550932224224 1101 46-47 -0.2344734579154064 1101 48-49 0.0316081085550195 1101 50-51 -0.0763218114297004 1101 52-53 0.027530683054799 1101 54-55 0.09338606908806213 1101 56-57 -0.07284187110157347 1101 58-59 -0.37523828232043854 1101 60-61 -0.34401220525676024 1101 62-63 -0.3412526236910054 1101 64-65 -0.3208460318389754 1101 66-67 -0.23389242880350736 1101 68-69 0.21532483567936822 1101 70-71 -0.3766115153324563 1101 72-73 -0.4602625650879695 1101 74-75 -0.4387047085541127 1101 76-77 0.08903197665258844 1101 78-79 -0.21704973145230255 1101 80-81 -0.20477443045391652 1101 82-83 -0.08049414072769068 1101 84-85 -0.05431906194959524 1101 86-87 -0.036829282447353506 1101 88-89 0.02744080120288217 1101 90-91 0.7999278253739028 1101 92-93 0.08595526439239976 1101 94-95 -0.6797044367279099 1101 96-97 -0.5854841473015355 1101 98-99 -0.04190921560397243 1101 100-101 -0.2081605078815798 1102 1 0.2790674789397869 1102 2 0.1993269880207933 1102 3 0.22279389596146615 1102 4 0.026281590907331065 1102 5 0.12132686557020378 1102 6 0.25261132841652767 1102 7 0.07750512262633436 1102 8 0.21192709140780153 1102 9 0.035080722423764144 1102 10-11 0.07327487191112425 1102 12-13 0.058572673528821895 1102 14-15 0.43950454632041414 1102 16-17 0.2759095077712672 1102 18-19 -0.03245545187050425 1102 20-21 0.33485152550987607 1102 22-23 0.1704746210514969 1102 24-25 0.32103097569378747 1102 26-27 0.16757214108918106 1102 28-29 0.05421788644630965 1102 30-31 0.036721096236888684 1102 32-33 0.12752886512969752 1102 34-35 0.0775972600727215 1102 36-37 0.6453036338205393 1102 38-39 0.7651796636414758 1102 40-41 0.8818816642200957 1102 42-43 0.5802691520346315 1102 44-45 1.0424865601788866 1102 46-47 1.6425205897036363 1102 48-49 1.405268822840732 1102 50-51 1.6908012044433107 1102 52-53 1.6066179846421065 1102 54-55 1.475499164326159 1102 56-57 1.6626740019142972 1102 58-59 1.5340275906954375 1102 60-61 1.2042417629972135 1102 62-63 1.0311084874201057 1102 64-65 1.491296825303884 1102 66-67 1.3041333648472815 1102 68-69 1.4272692801238094 1102 70-71 1.3726642783183358 1102 72-73 1.6407691809437743 1102 74-75 1.403120688271283 1102 76-77 1.2723752306208418 1102 78-79 1.2568193161667445 1102 80-81 1.7669716012921164 1102 82-83 1.34311697038342 1102 84-85 1.3719904618599301 1102 86-87 2.049966352473284 1102 88-89 1.9259626265997092 1102 90-91 2.1122294126754895 1102 92-93 1.1835544707416084 1102 94-95 1.2493332616847894 1102 96-97 1.5972936304762442 1102 98-99 0.955561022491267 1102 100-101 -0.5772974126434853 1103 1 -0.005377147534652238 1103 2 -0.10907733701196776 1103 3 0.07825752636775718 1103 4 -0.051372406471436705 1103 5 0.03658735049812378 1103 6 0.049670633790050545 1103 7 0.17166469012305896 1103 8 0.28200409009718896 1103 9 0.27078681678811023 1103 10-11 0.2598328666686598 1103 12-13 0.013991251510468317 1103 14-15 0.06814969704125673 1103 16-17 0.146383786932482 1103 18-19 0.19301145507576223 1103 20-21 0.011006505850637893 1103 22-23 0.03265515840405442 1103 24-25 0.11774214869510047 1103 26-27 0.19615995236048178 1103 28-29 -0.022637454313844785 1103 30-31 0.19710772795379938 1103 32-33 0.2734372858374243 1103 34-35 0.6052225877267219 1103 36-37 0.685441248499437 1103 38-39 0.44809250767816877 1103 40-41 0.7253040102227217 1103 42-43 0.9711194141578261 1103 44-45 0.9997686702706261 1103 46-47 1.2638140038582932 1103 48-49 1.0036264571788678 1103 50-51 1.0055538256752925 1103 52-53 1.1147274210772267 1103 54-55 1.1216123687822517 1103 56-57 1.0248135825171758 1103 58-59 1.0547107492799697 1103 60-61 1.4136126673222407 1103 62-63 0.7911428910898266 1103 64-65 0.7362181883445089 1103 66-67 1.1703481420425632 1103 68-69 0.9117876287476605 1103 70-71 1.1296474041374687 1103 72-73 0.9246813696724807 1103 74-75 0.19882842090562391 1103 76-77 0.6004183171214983 1103 78-79 0.8656414000461687 1103 80-81 1.10117867861846 1103 82-83 1.1636444933192003 1103 84-85 1.2023476047170725 1103 86-87 0.9360861100093238 1103 88-89 0.20809319671766602 1103 90-91 0.18382181110274942 1103 92-93 0.4553844183169673 1103 94-95 0.12181442157993772 1103 96-97 -0.11715591080815813 1103 98-99 -0.025701264533637413 1103 100-101 -1.457273657728674 1104 1 -0.1737217119578105 1104 2 -0.04664330275038253 1104 3 0.19699585550635135 1104 4 0.2326659145482921 1104 5 0.0040307214488422005 1104 6 0.018453616659257932 1104 7 0.09737712009788879 1104 8 0.12007342516254482 1104 9 0.177582302701893 1104 10-11 0.30458808303628615 1104 12-13 0.224087054059801 1104 14-15 0.2985832441712404 1104 16-17 0.2814207277459886 1104 18-19 0.21805813852140687 1104 20-21 0.09310847873364736 1104 22-23 0.31360831258120925 1104 24-25 0.3443598315724259 1104 26-27 0.3670822548692101 1104 28-29 0.32892948568777314 1104 30-31 0.2985731822040165 1104 32-33 0.4102603758755876 1104 34-35 0.4418711222724738 1104 36-37 0.6818080585993016 1104 38-39 0.6407327609866087 1104 40-41 0.37322964146408566 1104 42-43 -0.15602193519672625 1104 44-45 0.024510896462075493 1104 46-47 0.013550140904648345 1104 48-49 -0.11529454631224212 1104 50-51 0.023963340979342718 1104 52-53 0.18365654342844806 1104 54-55 -0.11056673327181699 1104 56-57 -0.16246822311731535 1104 58-59 0.09964864000011886 1104 60-61 0.18559448107559007 1104 62-63 0.13923901839356034 1104 64-65 -0.0948283327239352 1104 66-67 0.17974572262225053 1104 68-69 -0.0013811623540647133 1104 70-71 -0.09713265594209375 1104 72-73 0.16747588132304259 1104 74-75 -0.23546970363769404 1104 76-77 -0.3449011600239089 1104 78-79 0.02345174347394874 1104 80-81 0.5975657858686034 1104 82-83 0.30937803233032213 1104 84-85 0.15367503834539065 1104 86-87 -0.286573628563378 1104 88-89 0.2187289034644344 1104 90-91 0.34215988043781564 1104 92-93 0.6595737501347791 1104 94-95 0.6030192604205631 1104 96-97 0.08733787826385253 1104 98-99 -0.13010980557447382 1104 100-101 0.029391114537300922 1105 1 -0.04411975049744399 1105 2 0.08433781053162193 1105 3 0.01757203448960354 1105 4 0.029528344154080344 1105 5 -0.09417638118303984 1105 6 -0.02539191833672305 1105 7 0.022175036046249375 1105 8 -0.21082723759652566 1105 9 -0.018084861991823686 1105 10-11 -0.24071322332696354 1105 12-13 -0.14522873339758036 1105 14-15 -0.07984069177481956 1105 16-17 -0.0385520723153121 1105 18-19 0.12406511090005523 1105 20-21 0.17914265105100213 1105 22-23 0.004754924081801448 1105 24-25 -0.023327790539973137 1105 26-27 0.07368685970390487 1105 28-29 0.08905284315626716 1105 30-31 -0.18351699900120622 1105 32-33 -0.10454635132052914 1105 34-35 0.11175580985627676 1105 36-37 0.07116943468633963 1105 38-39 0.20809091905272936 1105 40-41 0.11091304950148384 1105 42-43 0.08933300484847706 1105 44-45 0.018812317754644425 1105 46-47 -0.18546912891108747 1105 48-49 0.022084299031206456 1105 50-51 0.02102306591518044 1105 52-53 0.06522188074600166 1105 54-55 -0.24886501316301946 1105 56-57 -0.5318606301203346 1105 58-59 -0.5832613703435285 1105 60-61 -0.20052014176469868 1105 62-63 -0.34105060348898775 1105 64-65 -0.5806728716658185 1105 66-67 -0.24517670008778225 1105 68-69 -0.5719190315645015 1105 70-71 -0.21655379527473784 1105 72-73 -0.3705944554198588 1105 74-75 -0.15261524246464475 1105 76-77 -0.2157876481670371 1105 78-79 -0.3312488656514354 1105 80-81 -0.2763184419979261 1105 82-83 -0.18366874390229526 1105 84-85 -0.46540131303184396 1105 86-87 -0.8188783444964152 1105 88-89 -0.7089805552184707 1105 90-91 -0.5430736176275381 1105 92-93 -0.620422801985665 1105 94-95 -0.3252744222978947 1105 96-97 -0.22251156432895058 1105 98-99 -0.6137706874654434 1105 100-101 -1.1448704645915342 1106 1 -0.1402094531445215 1106 2 -0.1813814428925511 1106 3 -0.08582729841792514 1106 4 0.003447867253932202 1106 5 -0.03067840375064179 1106 6 -0.010488788679495542 1106 7 0.0894342795349985 1106 8 0.13574149421810944 1106 9 0.4205461790982383 1106 10-11 0.26904477823431705 1106 12-13 0.07317308336488537 1106 14-15 0.14449576411901432 1106 16-17 0.10974293868461871 1106 18-19 -0.24286236053561083 1106 20-21 0.1777233053693621 1106 22-23 -0.014024793468401242 1106 24-25 -0.11657880182377056 1106 26-27 0.15483794905171067 1106 28-29 0.2482679449943177 1106 30-31 -0.1567800747232937 1106 32-33 -0.04881042058459428 1106 34-35 -0.11191532774928703 1106 36-37 -0.012483251425358333 1106 38-39 0.06538458167426597 1106 40-41 0.186989978037424 1106 42-43 -0.19234501658364422 1106 44-45 -0.2264683578539035 1106 46-47 -0.0971237311394546 1106 48-49 -0.1950312357072761 1106 50-51 -0.2930249079688636 1106 52-53 -0.37245110200895226 1106 54-55 -0.26838988719609347 1106 56-57 -0.40011873813254084 1106 58-59 -0.30284301820386617 1106 60-61 -0.19388470070302333 1106 62-63 -0.3669447912684163 1106 64-65 -0.4519116056094745 1106 66-67 -0.116424304941944 1106 68-69 -0.06555858872864917 1106 70-71 -0.19799585048728474 1106 72-73 -0.021701545051538318 1106 74-75 -0.10595425318070895 1106 76-77 -0.16223396141662505 1106 78-79 -0.011257381725528148 1106 80-81 0.01550790105792288 1106 82-83 -0.18454696404280924 1106 84-85 -0.18904583790587637 1106 86-87 -0.3663465866367872 1106 88-89 -0.37259562866961105 1106 90-91 -0.12898838591936013 1106 92-93 -0.5590538430757235 1106 94-95 -0.5771816680576052 1106 96-97 -0.7940998121467047 1106 98-99 -0.5162261554946816 1106 100-101 -1.1936249887558965 1107 1 0.03899012208611907 1107 2 -0.03256283268481042 1107 3 -0.008174149151315646 1107 4 0.15135388703225772 1107 5 -0.019777096257442395 1107 6 0.08428787555359918 1107 7 -0.10813325215677594 1107 8 0.05513487047084453 1107 9 0.2875568647025375 1107 10-11 0.13272036063293058 1107 12-13 -0.04801892566145938 1107 14-15 0.1490006423296748 1107 16-17 0.14968951066311575 1107 18-19 0.004325201686462776 1107 20-21 -0.027659318908867192 1107 22-23 0.0611809541920465 1107 24-25 -0.24928604281402045 1107 26-27 -0.19466759286107305 1107 28-29 0.022741961055913862 1107 30-31 0.0632537740853607 1107 32-33 0.022545854719048464 1107 34-35 0.010786603623913038 1107 36-37 0.06787448402297258 1107 38-39 0.1935703519005827 1107 40-41 0.14610592679962764 1107 42-43 -0.08823504114062075 1107 44-45 -0.16184036289803316 1107 46-47 0.04760481468917277 1107 48-49 0.1818105377048198 1107 50-51 0.34925623625360913 1107 52-53 0.6945662206166574 1107 54-55 0.463615488791163 1107 56-57 0.3294973101849692 1107 58-59 0.4471818706260322 1107 60-61 0.6244268410770246 1107 62-63 0.4705062606994588 1107 64-65 0.3036883811164941 1107 66-67 0.7705969998964477 1107 68-69 0.5818745432816996 1107 70-71 0.8179993708573754 1107 72-73 0.88433482582521 1107 74-75 0.7920015442574027 1107 76-77 0.4088070553750356 1107 78-79 1.0047046545126115 1107 80-81 1.203061826856029 1107 82-83 1.1682687922457689 1107 84-85 0.6608496290085704 1107 86-87 0.4521225843992518 1107 88-89 0.5115883495609559 1107 90-91 1.3241293548385897 1107 92-93 1.1063241352875863 1107 94-95 1.0417620500017328 1107 96-97 0.830593225617946 1107 98-99 0.8142311352732214 1107 100-101 0.23559117902800253 1108 1 0.016232657511217496 1108 2 -0.0015658691220608034 1108 3 0.20214657453289675 1108 4 0.12896016233590046 1108 5 -0.01594992014408092 1108 6 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2316 62-63 -0.7742208326932101 2316 64-65 -0.7819225190911041 2316 66-67 -0.7049279969495572 2316 68-69 -0.5392675470716597 2316 70-71 -0.8007915593991832 2316 72-73 -0.7344331662840666 2316 74-75 -0.5359222093410203 2316 76-77 -0.5251826912731303 2316 78-79 -0.6767475595920587 2316 80-81 -0.7073303007718224 2316 82-83 -0.687513341231309 2316 84-85 -0.8592671261611144 2316 86-87 -0.8799013630265158 2316 88-89 -1.3958390225302004 2316 90-91 -1.3525115828728715 2316 92-93 -1.4802329634955456 2316 94-95 -0.8592197027021022 2316 96-97 -1.4526355479940136 2316 98-99 -1.5871405659344724 2316 100-101 -0.9620037562290769 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 7.0 6 24.0 7 36.0 8 95.0 9 182.0 10 376.0 11 579.0 12 915.0 13 1230.0 14 1540.0 15 2149.0 16 2981.0 17 4580.0 18 4480.0 19 2362.0 20 1811.0 21 1842.0 22 1947.0 23 2124.0 24 2349.0 25 2676.0 26 3004.0 27 3459.0 28 3965.0 29 4809.0 30 5835.0 31 7047.0 32 8763.0 33 11285.0 34 15846.0 35 24369.0 36 36306.0 37 63368.0 38 41388.0 39 1548.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.23448714454438 23.805305714545288 15.982632264316646 17.977574876593682 2 13.846554005405629 24.946565691215664 39.71705261253247 21.48982769084624 3 19.766736153257515 29.859506406462632 33.41312354162976 16.9606338986501 4 10.543616343547736 20.378167891163642 42.307909785546535 26.770305979742083 5 9.508479751506904 42.352014294384404 35.737468853546645 12.40203710056205 6 22.909465129165895 42.52390879036788 18.44737050426155 16.119255576204676 7 22.16119632537819 39.14030134311423 22.907580321095903 15.790922010411679 8 22.877423391975995 33.551091492353336 21.142269082739304 22.429216032931368 9 24.01358569656852 20.492010298591296 25.231171709784796 30.263232295055392 10-11 21.714496812789555 28.655114049736312 28.740495855307053 20.889893282167076 12-13 25.877849358599814 28.462486664982904 28.011452093833284 17.648211882583997 14-15 22.55336393022401 29.166061953991573 30.330126000131933 17.950448115652478 16-17 16.445417859613343 32.56211961571881 31.535447244269616 19.45701528039823 18-19 16.139423022553615 32.284688950124206 34.73324311385372 16.84264491346846 20-21 17.99727833714693 29.85667919435764 33.2681818010472 18.877860667448235 22-23 17.44729134232261 29.671025599463206 33.284956592870145 19.596726465344034 24-25 18.624200604270605 30.63252393239558 33.58602374485682 17.157251718476996 26-27 18.662082666561115 28.558019078638598 36.20210313623264 16.57779511856765 28-29 16.303778286257113 30.55160792976451 35.95459874320998 17.1900150407684 30-31 17.08748148176071 30.440027292020854 34.77433192977959 17.698159296438842 32-33 17.113114871512632 30.6863717067691 34.56813392692222 17.632379494796044 34-35 18.43141024916023 29.236459051992263 34.2622647982477 18.06986590059981 36-37 18.683471276936707 28.644067796610166 35.330404781206994 17.34205614524613 38-39 18.169890036553937 30.083193671776176 34.61652298335938 17.130393308310506 40-41 16.811652117855736 30.352507855603918 35.72127355974312 17.114566466797232 42-43 16.326707571087926 31.091130149574465 33.85453483002045 18.72762744931716 44-45 17.494948504908244 30.25996350860262 33.08427703303828 19.16081095345085 46-47 18.755961020111965 31.047631613669353 31.663996380977512 18.532410985241174 48-49 18.1766262553153 30.467030067251727 32.48382792002171 18.87251575741126 50-51 17.13466627462511 30.981083994903457 33.61077812709686 18.27347160337457 52-53 17.334202858122957 32.39871983835886 32.167830849784565 18.099246453733617 54-55 16.657179799381026 30.617010769793314 32.71725240218789 20.008557028637775 56-57 18.36010389062082 31.644796610361166 30.92272663874638 19.07237286027164 58-59 18.694868740481613 30.16386706675312 31.639311509522155 19.50195268324311 60-61 17.89234211225688 30.848683726917862 32.589138461712444 18.66983569911281 62-63 18.489422337490012 30.40946033565042 32.23925270284534 18.861864624014235 64-65 17.785916098230324 31.61291417003896 32.07941208069377 18.521757651036943 66-67 17.513844228398668 30.67914940457566 31.04782620168656 20.759180165339103 68-69 16.83121475315902 30.924296571066677 31.855227841611626 20.38926083416267 70-71 18.293039202196937 31.181569558030525 30.895078191410157 19.630313048362382 72-73 18.211015572284275 29.01360454464922 32.046449210076936 20.72893067298957 74-75 17.188695675119405 31.92544453198331 30.714078385398015 20.171781407499275 76-77 17.90936086239619 29.728067494500603 31.549943591078712 20.812628052024497 78-79 17.446693799684432 29.43523987083226 32.28571482431717 20.832351505166145 80-81 17.822778702382593 30.178284331372502 31.32123409925795 20.67770286698696 82-83 17.0916280595147 30.329012096698193 31.699079082776997 20.880280761010106 84-85 16.942916702792154 29.45351874818587 31.115354023499787 22.488210525522188 86-87 17.054469782298217 29.64623224416409 31.5393076332407 21.759990340296998 88-89 17.893884378210746 28.6483039703008 31.106965197663573 22.35084645382489 90-91 17.26909226469258 27.830037827823794 32.41952362299341 22.481346284490215 92-93 17.721530916506772 29.315927759076292 31.78823050448773 21.174310819929207 94-95 17.277093173602132 28.677543265769245 31.459143015466733 22.586220545161886 96-97 16.663399666012012 28.056499006706147 31.30063065678022 23.979470670501623 98-99 17.077524186845146 28.183588191278226 30.339435725096454 24.399451896780178 100-101 17.441325703601503 27.079139619568902 31.76837129351096 23.71116338331863 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1435.0 1 892.5 2 392.5 3 416.0 4 347.5 5 348.5 6 584.0 7 1080.5 8 1620.5 9 1697.5 10 1234.5 11 878.0 12 805.0 13 780.5 14 778.5 15 722.0 16 687.5 17 693.5 18 728.5 19 805.5 20 878.0 21 1043.5 22 1189.5 23 1271.0 24 1400.0 25 1814.0 26 2779.5 27 3716.5 28 3828.0 29 3900.5 30 4699.0 31 5576.0 32 6721.0 33 8378.0 34 10473.5 35 11696.5 36 12624.0 37 14134.5 38 13619.5 39 11609.5 40 10166.5 41 9282.0 42 8419.0 43 7993.5 44 7895.5 45 7546.0 46 7562.0 47 9846.5 48 19511.0 49 18878.0 50 8306.5 51 4819.5 52 2743.0 53 1299.0 54 640.0 55 385.0 56 308.0 57 289.5 58 254.5 59 228.5 60 234.0 61 216.0 62 151.5 63 106.5 64 76.5 65 63.5 66 67.0 67 67.5 68 57.5 69 45.0 70 44.5 71 42.0 72 33.0 73 29.0 74 26.5 75 22.0 76 15.0 77 11.5 78 10.5 79 7.0 80 3.0 81 4.5 82 3.5 83 1.0 84 2.0 85 1.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41880435315271847 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.654424209982698E-4 16-17 0.0016963272629948094 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.8848080699942324E-4 26-27 0.003204173718990195 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010554925191967702 36-37 0.02789515943591464 38-39 0.020544407962937133 40-41 0.008481636314974046 42-43 0.009989482770969433 44-45 0.004146577753987311 46-47 0.005277462595983851 48-49 0.0026387312979919255 50-51 3.769616139988465E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0011308848419965395 60-61 0.0013193656489959627 62-63 0.0011308848419965395 64-65 0.0016963272629948094 66-67 0.002261769683993079 68-69 0.0026387312979919255 70-71 1.8848080699942324E-4 72-73 0.0 74-75 0.0 76-77 0.09424040349971162 78-79 0.016020868594950978 80-81 1.8848080699942324E-4 82-83 0.0 84-85 0.0 86-87 0.0981985004466995 88-89 0.38808198161181245 90-91 0.39788298357578245 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.8848080699942324E-4 100-101 3.769616139988465E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 265279.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.393027717987476 #Duplication Level Percentage of deduplicated Percentage of total 1 70.86896505285839 15.86970698773744 2 10.394922900814759 4.655475932885754 3 4.496330213453639 3.0205934129727567 4 2.58063430072049 2.311528617040927 5 1.7490404686553094 1.9583155847240075 6 1.2743249612820684 1.712159650782761 7 0.9275469665342401 1.453940945193551 8 0.7676250757524746 1.375155967867792 9 0.6110699616187462 1.2315335929342315 >10 5.248804794289947 24.17040172799204 >50 0.6430543397750993 10.131974261060996 >100 0.3787623729041816 15.63109028607617 >500 0.03535115480439028 5.768643579024348 >1k 0.023567436536260183 10.70947945370723 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3783 1.4260457857576363 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3480 1.3118264167159859 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2684 1.011764971972904 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2613 0.9850006973789859 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2287 0.8621112112153619 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2219 0.8364778214634404 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1917 0.7226354140357888 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1851 0.6977559475118649 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1674 0.631033741834069 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1568 0.5910758107501913 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1144 0.4312440864146804 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1112 0.4191813147667173 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1073 0.4044798118207623 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1005 0.3788464220688407 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 995 0.3750768059288523 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 957 0.3607522645968961 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 950 0.35811353329890416 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 947 0.35698264845690764 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 890 0.33549583645897335 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 853 0.32154825674101606 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 846 0.31890952544302414 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 837 0.3155168709170345 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 800 0.3015692911990772 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 695 0.2619883217291983 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 687 0.2589726288172075 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 674 0.25407212783522254 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 640 0.24125543295926175 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 614 0.23145443099529175 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 602 0.2269308916273056 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 589 0.22203039064532057 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 588 0.22165342903132176 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 553 0.2084597725413621 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 550 0.20732888769936555 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 529 0.1994126938053898 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 507 0.19111953829741518 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 464 0.17491018889546478 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 462 0.1741562656674671 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 461 0.17377930405346825 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 453 0.17076361114147745 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 448 0.16887880307148323 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 446 0.16812487984348554 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 438 0.16510918693149476 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 426 0.16058564756350863 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 422 0.15907780110751324 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 398 0.15003072237154091 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 393 0.14814591430154667 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 377 0.14211452847756514 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 365 0.13759098910957895 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 356 0.13419833458358935 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 341 0.12854391037360666 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 341 0.12854391037360666 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 340 0.12816694875960782 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 340 0.12816694875960782 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 339 0.12778998714560896 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 337 0.12703606391761127 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 328 0.12364340939162166 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 322 0.12138163970762857 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 320 0.12062771647963087 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 317 0.11949683163763433 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 315 0.11874290840963664 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 313 0.11798898518163894 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 307 0.11572721549764588 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 304 0.11459633065564934 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 303 0.11421936904165049 No Hit CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC 302 0.11384240742765164 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 301 0.1134654458136528 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 294 0.11082671451566088 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 282 0.1063031751476747 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 281 0.10592621353367586 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 274 0.10328748223568393 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 269 0.10140267416568971 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.654424209982698E-4 0.0 24-25 0.0 0.0 0.0 0.0035811353329890418 0.0 26-27 0.0 0.0 0.0 0.010931886805966549 0.0 28-29 0.0 0.0 0.0 0.028083640242914064 0.0 30-31 0.0 0.0 0.0 0.07407295715077333 0.0 32-33 0.0 0.0 0.0 0.14060668202156973 0.0 34-35 0.0 0.0 0.0 0.2521873197652283 0.0 36-37 0.0 0.0 0.0 0.4048567734347611 0.0 38-39 0.0 0.0 0.0 0.6054003520821475 0.0 40-41 0.0 0.0 0.0 0.8347814942004457 0.0 42-43 0.0 0.0 0.0 1.0419219010928118 0.0 44-45 0.0 0.0 0.0 1.265083176580129 0.0 46-47 0.0 0.0 0.0 1.5772073929711738 0.0 48-49 0.0 0.0 0.0 1.8817923770822418 0.0 50-51 0.0 0.0 0.0 2.2172882135412153 0.0 52-53 0.0 0.0 0.0 2.52281560168728 0.0 54-55 0.0 0.0 0.0 2.850583725059277 0.0 56-57 0.0 0.0 0.0 3.243189246039076 0.0 58-59 0.0 0.0 0.0 3.6904542010487074 0.0 60-61 0.0 0.0 0.0 4.216127171770099 0.0 62-63 0.0 0.0 0.0 4.696753229618627 0.0 64-65 0.0 0.0 0.0 5.1581542451532165 0.0 66-67 0.0 0.0 0.0 5.660455595806679 0.0 68-69 0.0 0.0 0.0 6.25153140655687 0.0 70-71 0.0 0.0 0.0 6.950041277296733 0.0 72-73 0.0 0.0 0.0 7.6494935520715925 0.0 74-75 0.0 0.0 0.0 8.414537147682251 0.0 76-77 0.0 0.0 0.0 9.136230157683043 0.0 78-79 0.0 0.0 0.0 10.010027178932368 0.0 80-81 0.0 0.0 0.0 10.946965270526501 0.0 82-83 0.0 0.0 0.0 11.78438549602494 0.0 84-85 0.0 0.0 0.0 12.767501385333931 0.0 86-87 0.0 0.0 0.0 13.799622284462775 0.0 88-89 0.0 0.0 0.0 14.785376905069757 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCATAC 15 6.639612E-4 94.90141 8 ATTGCCG 25 0.005037689 56.94085 8 GGATATG 160 0.0 47.68441 1 GTAAGCG 40 4.2491592E-9 47.459652 94-95 ACCCTTG 40 5.4082536E-4 47.450706 7 GACATAT 55 4.840912E-5 43.349464 1 TGTAGAT 55 4.9833863E-5 43.13701 5 GATTGGC 180 0.0 39.542255 9 GCAGATC 75 6.951537E-6 37.960564 8 AGCAGAT 75 6.951537E-6 37.960564 7 GCTTGTC 45 5.7282523E-7 36.913063 94-95 AGACGGC 35 2.0621142E-4 33.899754 94-95 GTAGATA 70 2.061627E-4 33.893364 6 TAGATAT 70 2.061627E-4 33.893364 7 ATATGAG 200 0.0 33.215496 3 GATATGA 205 0.0 32.405357 2 AGATTGG 220 0.0 32.352757 8 GTCCCAG 30 0.003965512 31.639767 62-63 TGGGACC 60 0.003965627 31.633806 6 GCATCAT 60 0.003965627 31.633806 7 >>END_MODULE