FastQCFastQC Report
Fri 27 May 2016
SRR939151_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939151_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257506
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65130.28850421660008874No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58470.25900263388004285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53480.2368985951753838No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32080.14210371976862965No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30860.13669952593702964No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23820.10551466972845255No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23190.10272397947115092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG80750.024.5737381
TACATGG81100.023.8898932
ACATGGG81450.022.7403643
GTACCTG18950.022.045041
GTATCAA136450.021.8485981
GAGTACT68500.020.84202812-13
TACCTGG20550.020.2853552
AGTACTT72200.020.0690812-13
CTAGTAC4550.019.7812613
GTACTTT72150.019.69138714-15
AGAGTAC114650.019.4120710-11
GGTATCA106600.018.748321
ATCAACG157600.018.7259393
TCAACGC158150.018.6308614
GTGTAGC12000.018.5931971
TATCAAC159500.018.591972
CAACGCA158700.018.5662945
AACGCAG161600.018.2563516
GTATAGC7650.017.995921
CATGGGG47200.017.9648154
Produced by FastQC (version 0.11.5)